MD05G1115300.v1.1

Urb2/Npa2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000376
Physical Location & Seq
Forward (+)
23645404 .. 23656216
10813 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1115300.v1.1.491

Sequence Viewer

Length: 6354 bp
ATGGCCGGCTCCGAAGCCACCCCTAAAGCTCAACGATCGAAGAACAAAAAGAGAAAGCAGAGGAGTCTCGGAGCATCCGAAAGACCTTCCAAAACGCAGCGTATTAATGTCTCAGAGAAGGAGAGTGAACCGCAAGAAATGATTGAAGTTGGCGCAGATGAAAAGCACGAGGCGAGAGAACCCAGTCACGAGCTCGAAGAAGGCGGTCCATGGCGGAACCTGGAGTTGGTTTTGTCAATCCAGAACAAAGAAATTGATCTTGAGAAGAAGGTGGAACTAGCTTATGGTTTTGTGATATCTAGAGTGAAAGAAGAAGGCGGCAAGAGTAACCAGGATACTCAAGCAGTCAGTATGTCACGTTTAATAATTTTTGTCAATGATTGGATCCAGTCATTGTTGATTTCTTCAGGAAAGAAAATTCAGAGTGGTGGAGAGAAGCATCAAGCCGAAGTTATTGACACATATTTGGATGTTAGATGCTGGGAAATTTTCAAATTCTGTTTGGAGGAGTCTTTGAAAGTGAATGTTTCTCTAAATTTCTCACGGAACCTTTTACGTTCTATTGATTTGATCGCAAGAAATGCACTGTCCTTATTGAACAAAACATCTTCACGCCCAACTTTGTTTTCCAGCAGTGAAGGGTTACAATTGTACAATACTGTGCTTGATTGCATAGCTTTGGTGTTTTCATCCCATGGAGGCTTATCAAATGAAAATTTAGATCTGTGGGTTTCAACTGTAGGTGCAGTGCTTGATCTAGTCCACAAATTTTACAGTGAAAATCTTGCCTATGGCAACGGAGGTCACTTTGTTTTCCGATTTGTGAGCTTGGTGTTTGAGCCATTTGCCAAGTTTTTGAGGGCCCACCCAGCTCGGAAGAAGGGATTTCGTGATTTTATCGATAAACTCCTTGAGCCTCTGTTGCATCTCTTGGGTCTCTTGCATCTTCAGATTGAGGGAATTAATCCTGATTGGACAAGAAACTTATTGAAGCTAGTTGAAGAAGTATTATCTCATGGGCTATATCACCCAATCCACATTGATGGATTTTTGAGCTTGTGCAGTAGTGAAAAATATGCCACATCCAATTCTGGGAAGTCAAAGGACTCCAAAACAACGCTCAAGAGTTATCACAGACATTTATTTGATAAACTGGAAAGAATTTTAGCTGGAAAGAATGCTTTGGCAGTGGAAAGCATAGGAGAACTATTTCGCTTGCTCATCGATCAAGTTAAAAAGCTAAAAAGGGCTTCAGTGCTGACTGAAAATACCAAAATGATGGGGAAGACTGAAGGTTCAACTAGTACTACACTAGCTGAAAAGAATTACTGCTCAACTAGCTTCAATGCGGAAACACGAAAGTCACTTCTTGATTTTTTTGTACTGATTATGGAGCCTCTTCTGCTTGAGATTAACGGCTATCTTGAAGCTAAGCTGGAAATGGGACCTGTATTGTTGGATGTTCATTGCACACTTAAATCTGTTAATAATTTAGTTTTTGGCTTTATGCATGAAAAGGTTTATGTGAGAACGGAGGATACATCTGCTGGAGCTTGCCTTAATTTCTTGAAGAAGGTTTATAATATGATTATATCATTGTCCTCCAATTTAATTCAGTCATCGAAATATGATGTAGTTAACGGGACACACGTGGATGCTCTAACTTTAATAGCCAATGAGGTGCTTTCTGCTGTGGGGTATCTATTGGAAATTGAATATGAGGTTATAGAGAATGATTTGGTAAGCTTGTGGCTTCTGATGCTTTCTCACTTAGCCATTGGGCTTTCACTGATGGATGTTCCGGATCGGTGCTCATTATCTTTCAAGATAAACGATATTGGATGCCAATTAATTGTTCTCTATAGTCAACTTCGCCAGGTGAATAATACTATTTTTGCATTGTGTAAAGCAATAAGGCTTCTAAGTTCACATAATGGTGATGGTGAACGAAACTATACTAGATTTGTGATTTCTTTACATGGTGAAGCTTATGCAAAATCAGTGGAAATGTTATTATGCTCGCAAGAGTTTAAGATTGCAATCCAACAAGCTATAAATTCCATACCAGAAGGCCAAGCAAGTGGGTGTATTGGGCAGTTAACTGTCGATATATCAGAAACTCTAGAGTGGATGAAAATTAGTTGTTCAAAATCTGATGAAAAGGAAGTTGACAAATGGGATGTGCAAAGTAGCTCGCATGGTTTTAATCTGGAAGCTGAGCTTTTGGGGAGAGGGTTGTCTGAAATGTATGCACTAGTGCTAGACTCACTATTTGCCACACCTGGAAACTGTAATCTCCTTGGAGTTTCTATAAAGGACCTTATAGCAGTACTTTTTCCTTGCATGGGCCGTCTGGTTTCACAAAAGCCAGATTCTTTAAACAAGTTCCTTTTTTCTGTCACAGGAAAAGGTTTTGATAATGAGACAGCTGAGAACAAAACTAATTTGCTGATATTTGGATTGTCCACTCATTGGGTTTTTGTGTTCTTCTTTCGATTGTATATGTCCTGCCAAAGCTTATATAGGTCAGCAACGAGCCTTATGCCTCCAGATTTATCAAGAAAGATGTCAGCAGCAATGGGAGATTCATTCACATCATACTCTGGTAATGATTGGATGGAGATGACTGACTGGACCAGTGGGGGATATTTTTCTTGGATCAAGCAACCTTCAGCTCCTCTTCTTGTTGTTATTCAATCCATTTCAAACATCTATTGTAAGAACTGTGCGACAGATTCTTGGTCTTTGACTTATGTAATGCATGCTATGGCTTTTAGAAGGCTTGTTGATTTGAATAGGCATATAAAGTCTTCTGAATATGTGATGCAACATAACAAAAATCTGGTGCAAGTCAGGTTGTCTGAAGATGCTGGCTTGTCAAGGTGTCGTAAAAGAATCAAACAGTTGGAAAGGCACATCTCTGCCTTGAAGGAAGAGGCGGCAGGTCTTGCTGGTTTTCTGATGGAGCACCTATCATTAGTGTCTGAAGATCAACGGCCAATCTTCACTTCTGATGATACAAGTTGTACTAAAATGGTTGCCCATGAAACTGATGAATGGGATTTCAGTGTTTCTGCTTTGAACAAGAAGTCACTGCCAACTGCAATTTGGTGGATTCTTTGCCACAATATTGATACCTGGTGCACTCATGCGACTAAAAAGAATTTGAAGACATTCCTTTCTCTTTTAATCCAAACTTCCCTTCCCTGTGTAAGAAGCAGCTTTGGGGTGGTTAGAGAGCACAATAACCATGTAGCTGACAGGATGAAGAAAGTAACCTTGCATCAAATCTCATCACATTGCTTCATTGACTCCATTCTGTATGAGCAAAGATTTGTCTGCAGGCATTTTGCATCAAGTTTTTGCCGTGCATTGGAGAAATCGACAATACAATTCATCAGTGACTTTTCATCTGGCGATATTGATTTCAAATCATCACCAAATTGGCCGAAAGTTTTAAATGACCTGGAGAACTCATCAGTGGTTGTTTCCTCTAATAAACATAACATATTTGACCGTTCCTCTGTAGCAAATCCGGTCACTCGTGCTTCTGATAAGCTGCTTACAGGAAGTTGCAAGGAACAAAATGCCCTACCATTGACCATCATGAAATTTACAACTTGCCAGAGTTTACTTAATCTTTTGTGCCATATGCCAAAAGGTCATTTGAGTTCAAGATCAATTTCACTTTATGTGACTTCTATTCTCAACCTTGAAAGGCTTCTTGTTGGCGGCTTATTAGATTGTCAGAATGCATTGAACTCACATCATTATCACGAGCTCTTCAGATTGTTTGTGTCTTGCCGGAAGGCCTTAAAATGCGTAATTCTGGCTTGTGAAGAGAAGATGGCAGCTTGTCAAACCTCACATGCTTCAGTGTTACTTGAGGATGCATTTCCTGTGTCATGGCTTTACAAGTCAGTTTATGCGGTTCTTGGGATTCAAGAGTCATTTTCAAAAGATAATCAACTCCCATTTAATGACATGATCTTGTCGCTGATGGATCACACATTTTATGTGTTTCTGACATCAAGTAAGTATCAACTTAATCATGTCGATCATCGTCCTAAAGCTGCCAAACTGAATGCTGAAATTGTCCATGAACATAGTAATTCAAGTGAATCTGATCAGTGCTTGGATCCTTCCAACTCTATTGATGCTAGGAAAAGTGTAGCCATTATTGCTAAGAGTTTGACGGAACAGATGCAAAGCTTACTATTGACTTTGAAGAATGGCCCTTATAACAGAAAAGTGGGGATTGATGTTGATGCTCTGAATTTGAACAAGTTTTCATCCTTAGTTTCTTGCTTTAGTGGGTATTTATGGGGCCTAGTATGTGTTGTGAACCATACAAATGTGAGAAATAGTGATCATGAAGCAAACTCATCAAGGCGGAAACTTGAATCCATCACCGAACTCAACCTCTGTATAAATGTTTTTGCAGAGTTTTCTAGTCTTCTCTTACAAATGTTGCTTTTCGACGATAAACAACAGTCCAGAACTTTATGTGATGCTCAAAATATTCAAAAGACAAAGGTAGAGTCCAGTGCAGAGGATTTTATACCACAAGGCACTGGTGTTGAGACTAATATTGCTTGTGGTGGACTGCATAATGAATCTGGAGTTGCAATGACCTGCTCAGCATCACCTGACATCCATGATGACTCAGGATCTGGTAGTGTCCGTAGAAGAAAGTTGCACTTGAAAGGTGCTGTTTTGGCTGCCAGTGCACTGAGTGATGTTGATTCATTTGAATTGCAATCTTTAAATAAGCCTTTGTTGAGACGCCTGCTAAAAGGTGATTACCCAGACGCAGCATTTTTGCTCAGGCAGTTGTTTATTGCATCTTCAGCTATTTTGAGGTTGAGTTTGCATATGAATAGTGCTCCCCTGTCCTCAAGCTTGGTGCATACTTTTACTGGCATCACACAAGTCCTGTTATTGGAATCAGTGGACATGAATCATGTGCCACACTTTTTTTATTTTGTGTGTTTAGACGGTGTTCTGAAGTATCTGGAAGAATTGGCCAAGCATTTTCCTCTGACTAATCCTACGCTATCCAGAAATTTGTATGACAAGATGATTCGGCTACAGTTAAGGGCTTTGGGGAAATGCATAACTTTACAGGGAAAAAGAGCCACCCTAGTATCTCATGAGACAGAGTCAAGCACCAAAATGCTCCATTCTCCTATGGGATTTTCTGAGGCATCTCTGTCTGGCTGGCCATACTTATTAGATGAACTTAAAGCTAGGTTGAGGTCATCGTTCACAGTGTTCATAGAGAGACCATCAGAGTTGCATCTTTTATCAGCTTTACAAGCTATAGAGAGAGCACTAGTTGGTGTGCGGGAAGGGTGCACCATGAGTTATGACATACATACTGGAAGTGCAGATGGGGGAAAGGTTTCTTCAGTTGTTGCAGCTGGCATTGATTGCTTGGATTTGGTTCTGGAATTTGTTTCAGGACACAAACGTTTGAAGGTTGTTAAAAAATACATCCAGAGCTTCATTGCTAGTGTGTTCAATGTTATTCTACACTTGCAGAGTCCACTAATCTTTTATGAGCGAGTGGTCCAGAGTAAAGGTGATACTGATACTGATACTGATCCAGATCCAGGAACAGTTATTCTTATGTGTGTTGATGTACTGGTTAGAATTTCTGGCAAACATGCTCTGTACCAAATGGAGCCCTGGCATGTAGCACAATCTTTACGTATACCTTCAGCACTTTTTCAAGACTTCCATCTCTTAAAACTTTCAGAAGCTCCCATTCCTGATGATTCTTCAACAATTCCAAATAACCAATTCTCTAATCCAGTAGCAAGTTTACATTTCTCTGGCATAGATAGGCAATACTCGGTTGACCTATTTTCTGCTTGCTGCCGATTGTTACATAATGTTCTGAAGCATCACAAAAGTGAATGCGAACGGTGCGTTGCTATACTTGAAGCTTCTGTTCGTGTACTTCTTCATTGTTTGGAGACAGTGGATGCTGATGCAGTCGTCAAGAAAGGTTTCTTTTCATGGGAAGTGGCTGCTGGAGTCAAATGTGCTGGTAGTCTCCGAAGGATTTACGAAGAGATAAGACATCAAAAAGATGTCTTTGGTCCGCACTGTGCCCAATTTTTATCTAATTACATATGGGTTTATTCAGGATATGGCCCCCATAAAACAGGCATCAAAAGGGAAATAGATGAAGCTCTAAGACCAGGTGTATACGCGCTAATAGATACTTGCTCACCAGACGATCTTCAACGTCTTCATACCATTTTCGGAGAGGGTCCTTGCAGAAACACTTTGGCAACCTTAAAACATGATTACGAACTCAATTTCCAATACCAGGGAAAAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Urb2

Protein Analysis

2118

Amino Acids

237.2

Weight (kDa)

6.84

Isoelectric Point (pI)

42.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Urb2 PF10441 1878 - 2116 2.7e-37 Urb2/Npa2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000376)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30150 AT4G30150
fragaria_vesca FvH4_2g02080 FvH4_2g02080
malus_domestica MD05G1115300.v1.1 MD10G1117200.v1.1 MD10G1117300.v1.1 MD10G1117400.v1.1 MD10G1117500.v1.1 MD10G1117600.v1.1 MD10G1117700.v1.1
prunus_persica Prupe.8G159900_v2.0.a1 Prupe.8G159900_v2.0.a1 Prupe.8G159900_v2.0.a1
pyrus_communis pycom05g10980 pycom10g10170
rosa_chinensis RchiOBHm_Chr2g0118221 RchiOBHm_Chr6g0244891 RchiOBHm_Chr6g0244901 RchiOBHm_Chr6g0244911 RchiOBHm_Chr6g0244921 RchiOBHm_Chr6g0244931 RchiOBHm_Chr6g0244961 RchiOBHm_Chr6g0244971 RchiOBHm_Chr6g0245301 RchiOBHm_Chr6g0245311 RchiOBHm_Chr6g0245351 RchiOBHm_Chr6g0245361 RchiOBHm_Chr6g0245441 RchiOBHm_Chr6g0245521 RchiOBHm_Chr6g0245531 RchiOBHm_Chr6g0245571
rosa_laevigata RLG00000015303
rosa_multiflora Rmu_sc0000471.1_g000006 Rmu_sc0000471.1_g000007 Rmu_sc0000471.1_g000008 Rmu_sc0001288.1_g000012 Rmu_sc0001925.1_g000002 Rmu_sc0002202.1_g000009 Rmu_sc0002202.1_g000016 Rmu_sc0002202.1_g000017 Rmu_sc0002202.1_g000019 Rmu_sc0002202.1_g000020 Rmu_ssc0000087.1_g000055 Rmu_ssc0000087.1_g000056
rosa_roxburghii Rroxscaffold_3G00242140 Rroxscaffold_7G00215270
rosa_rugosa Rorug05G0511800 Rorug05G0513500 Rorug05G0513900 Rorug05G0514000 Rorug05G0514200 Rorug05G0514400
rosa_samantha Rh1AG142300 Rh1DG147200 Rh6AG026300 Rh6AG026400 Rh6AG026500 Rh6AG028900 Rh6AG029000 Rh6AG029100 Rh6AG029200 Rh6AG029300 Rh6AG029400 Rh6AG029500 Rh6BG025200 Rh6CG020200 Rh6CG020300 Rh6CG020400 Rh6CG023100 Rh6CG023300 Rh6CG023500 Rh6CG023800 Rh6CG024100 Rh6CG024200 Rh6CG024400 Rh6CG024700 Rh6CG024800 Rh6CG025200 Rh6CG025400 Rh6CG025600 Rh6CG025700 Rh6CG025800 Rh6CG025900 Rh6CG026000 Rh6DG020800 Rh6DG020900 Rh6DG021000 Rh6DG023100 Rh6DG023300 Rh6DG023500 Rh6DG023700 Rh6DG023900 Rh6DG024200 Rh6DG024500 Rh6DG024700 Rh6DG024900 Rh6DG025000 Rh6DG025200 Rh6DG025300 Rh6DG025400
rosa_wichuraiana Rw2G021630 Rw2G021750 Rw6G002430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1581, 4242
Acc36I ACCTGC 2 cut(s) 2933, 4643
AccB7I CCANNNNNTGG 1 cut(s) 2472
AccI GTMKAC 2 cut(s) 5716, 6216
AccII CGCG 1 cut(s) 6221
AccIII TCCGGA 1 cut(s) 1801
AclI AACGTT 1 cut(s) 5473
AcoI YGGCCR 5 cut(s) 3, 2996, 3446, 5025, 5222
AcvI CACGTG 1 cut(s) 1651
AcyI GRCGYC 1 cut(s) 4786
AdeI CACNNNGTG 1 cut(s) 4736
AfaI GTAC 8 cut(s) 653, 1306, 1383, 2331, 3028, 5646, 5678, 5964
AfiI CCNNNNNNNGG 8 cut(s) 226, 1092, 2345, 2472, 3373, 4942, 5615, 6340
AflIII ACRYGT 1 cut(s) 1648
AhlI ACTAGT 3 cut(s) 1301, 2254, 5335
AleI CACNNNNGTG 1 cut(s) 5916
AloI GAACNNNNNNTCC 2 cut(s) 6315, 6347
Alw44I GTGCAC 3 cut(s) 3142, 4728, 5356
AlwNI CAGNNNCTG 1 cut(s) 4673
Aor13HI TCCGGA 1 cut(s) 1801
ApaI GGGCCC 1 cut(s) 865
ApaLI GTGCAC 3 cut(s) 3142, 4728, 5356
ArsI GACNNNNNNTTYG 4 cut(s) 2255, 2287, 4526, 4558
AseI ATTAAT 3 cut(s) 105, 963, 1850
Asp700I GAANNNNTTC 5 cut(s) 1209, 3173, 3720, 5404, 6014
AspLEI GCGC 2 cut(s) 155, 6223
AvaII GGWCC 7 cut(s) 206, 1445, 2317, 2634, 5572, 6107, 6281
BaeGI GKGCMC 5 cut(s) 865, 3146, 4732, 5360, 6121
BaeI ACNNNNGTAYC 2 cut(s) 5130, 5163
BalI TGGCCA 2 cut(s) 5027, 5224
BamHI GGATCC 2 cut(s) 384, 4138
BanII GRGCYC 4 cut(s) 195, 865, 3783, 5691
BauI CACGAG 4 cut(s) 167, 188, 3543, 3776
BbrPI CACGTG 1 cut(s) 1651
BbsI GAAGAC 5 cut(s) 1292, 2802, 3176, 4448, 6251
BceAI ACGGC 4 cut(s) 1432, 2334, 3011, 3351
BcgI CGANNNNNNTGC 2 cut(s) 2524, 2558
BciVI GTATCC 2 cut(s) 328, 1531
BclI TGATCA 2 cut(s) 4126, 4369
BcuI ACTAGT 3 cut(s) 1301, 2254, 5335
BfmI CTRYAG 6 cut(s) 738, 1861, 3340, 3525, 5090, 5322
BfuAI ACCTGC 2 cut(s) 2933, 4643
BfuI GTATCC 2 cut(s) 328, 1531
BglII AGATCT 1 cut(s) 721
BlpI GCTNAGC 3 cut(s) 1431, 2217, 4639
BmcAI AGTACT 2 cut(s) 1306, 2331
Bme18I GGWCC 7 cut(s) 206, 1445, 2317, 2634, 5572, 6107, 6281
BmrI ACTGGG 1 cut(s) 177
BmuI ACTGGG 1 cut(s) 177
BpiI GAAGAC 5 cut(s) 1292, 2802, 3176, 4448, 6251
BpmI CTGGAG 6 cut(s) 242, 1569, 2532, 3488, 4641, 6060
Bpu10I CCTNAGC 1 cut(s) 4826
Bpu1102I GCTNAGC 3 cut(s) 1431, 2217, 4639
BpuEI CTTGAG 7 cut(s) 281, 324, 932, 1106, 1427, 3905, 4882
Bsa29I ATCGAT 2 cut(s) 900, 1224
BsaAI YACGTR 2 cut(s) 1651, 5714
BsaBI GATNNNNATC 3 cut(s) 2039, 5604, 5610
BsaHI GRCGYC 1 cut(s) 4786
BsaI GGTCTC 2 cut(s) 941, 5278
BsaJI CCNNGG 5 cut(s) 209, 694, 2299, 5690, 6340
BsaWI WCCGGW 2 cut(s) 1801, 3535
Bsc4I CCNNNNNNNGG 8 cut(s) 226, 1092, 2345, 2472, 3373, 4942, 5615, 6340
Bse118I RCCGGY 1 cut(s) 5
Bse3DI GCAATG 5 cut(s) 1465, 2583, 3298, 4635, 5508
Bse8I GATNNNNATC 3 cut(s) 2039, 5604, 5610
BseAI TCCGGA 1 cut(s) 1801
BseCI ATCGAT 2 cut(s) 900, 1224
BseDI CCNNGG 5 cut(s) 209, 694, 2299, 5690, 6340
BseJI GATNNNNATC 3 cut(s) 2039, 5604, 5610
BseLI CCNNNNNNNGG 8 cut(s) 226, 1092, 2345, 2472, 3373, 4942, 5615, 6340
BseMI GCAATG 5 cut(s) 1465, 2583, 3298, 4635, 5508
BseMII CTCAG 8 cut(s) 126, 2208, 2421, 4653, 4680, 4724, 4840, 5193
BseRI GAGGAG 3 cut(s) 76, 521, 2667
BseSI GKGCMC 5 cut(s) 865, 3146, 4732, 5360, 6121
BseYI CCCAGC 2 cut(s) 480, 868
BsgI GTGCAG 4 cut(s) 765, 1081, 4569, 5409
Bsh1236I CGCG 1 cut(s) 6221
Bsh1285I CGRYCG 1 cut(s) 38
BshVI ATCGAT 2 cut(s) 900, 1224
BsiEI CGRYCG 1 cut(s) 38
BsiSI CCGG 4 cut(s) 6, 1802, 3536, 3805
BslFI GGGAC 2 cut(s) 1458, 1657
BslI CCNNNNNNNGG 8 cut(s) 226, 1092, 2345, 2472, 3373, 4942, 5615, 6340
BsmBI CGTCTC 1 cut(s) 4777
BsmFI GGGAC 2 cut(s) 1458, 1657
BsmI GAATGC 4 cut(s) 1183, 3757, 4090, 5927
Bso31I GGTCTC 2 cut(s) 941, 5278
Bsp120I GGGCCC 1 cut(s) 861
Bsp13I TCCGGA 1 cut(s) 1801
Bsp1407I TGTACA 1 cut(s) 651
Bsp1720I GCTNAGC 3 cut(s) 1431, 2217, 4639
Bsp19I CCATGG 2 cut(s) 209, 694
BspCNI CTCAG 8 cut(s) 125, 2209, 2422, 4652, 4679, 4725, 4839, 5194
BspDI ATCGAT 2 cut(s) 900, 1224
BspEI TCCGGA 1 cut(s) 1801
BspFNI CGCG 1 cut(s) 6221
BspHI TCATGA 3 cut(s) 3606, 4372, 5152
BspMAI CTGCAG 1 cut(s) 3344
BspMI ACCTGC 2 cut(s) 2933, 4643
BspQI GCTCTTC 1 cut(s) 3788
BspTNI GGTCTC 2 cut(s) 941, 5278
BsrDI GCAATG 5 cut(s) 1465, 2583, 3298, 4635, 5508
BsrFI RCCGGY 1 cut(s) 5
BsrGI TGTACA 1 cut(s) 651
BssAI RCCGGY 1 cut(s) 5
BssECI CCNNGG 5 cut(s) 209, 694, 2299, 5690, 6340
BssNAI GTATAC 2 cut(s) 5717, 6217
BssNI GRCGYC 1 cut(s) 4786
BssSI CACGAG 4 cut(s) 167, 188, 3543, 3776
BssT1I CCWWGG 3 cut(s) 209, 694, 2299
Bst1107I GTATAC 2 cut(s) 5717, 6217
Bst2BI CACGAG 4 cut(s) 167, 188, 3543, 3776
Bst6I CTCTTC 6 cut(s) 1404, 2685, 2928, 3788, 3834, 6072
BstACI GRCGYC 1 cut(s) 4786
BstAPI GCANNNNNTGC 3 cut(s) 581, 2948, 5433
BstAUI TGTACA 1 cut(s) 651
BstBAI YACGTR 2 cut(s) 1651, 5714
BstDSI CCRYGG 2 cut(s) 209, 694
BstFNI CGCG 1 cut(s) 6221
BstHHI GCGC 2 cut(s) 155, 6223
BstMCI CGRYCG 1 cut(s) 38
BstNSI RCATGY 4 cut(s) 2765, 3872, 5672, 5699
BstSFI CTRYAG 6 cut(s) 738, 1861, 3340, 3525, 5090, 5322
BstSLI GKGCMC 5 cut(s) 865, 3146, 4732, 5360, 6121
BstSNI TACGTA 1 cut(s) 5714
BstUI CGCG 1 cut(s) 6221
BstV2I GAAGAC 5 cut(s) 1292, 2802, 3176, 4448, 6251
BstX2I RGATCY 5 cut(s) 384, 721, 4138, 4670, 5611
BstXI CCANNNNNNTGG 3 cut(s) 1043, 1279, 2081
BstYI RGATCY 5 cut(s) 384, 721, 4138, 4670, 5611
BstZ17I GTATAC 2 cut(s) 5717, 6217
Bsu15I ATCGAT 2 cut(s) 900, 1224
BsuI GTATCC 2 cut(s) 328, 1531
BsuTUI ATCGAT 2 cut(s) 900, 1224
BtgI CCRYGG 2 cut(s) 209, 694
BtsI GCAGTG 4 cut(s) 640, 753, 1194, 3092
BveI ACCTGC 2 cut(s) 2933, 4643
CaiI CAGNNNCTG 1 cut(s) 4673
CciI TCATGA 3 cut(s) 3606, 4372, 5152
CfoI GCGC 2 cut(s) 155, 6223
Cfr10I RCCGGY 1 cut(s) 5
ClaI ATCGAT 2 cut(s) 900, 1224
CseI GACGC 2 cut(s) 4794, 4820
CsiI ACCWGGT 2 cut(s) 3137, 6208
Csp6I GTAC 8 cut(s) 652, 1305, 1382, 2330, 3027, 5645, 5677, 5963
CspCI CAANNNNNGTGG 2 cut(s) 1983, 2018
CviQI GTAC 8 cut(s) 652, 1305, 1382, 2330, 3027, 5645, 5677, 5963
DraI TTTAAA 3 cut(s) 2379, 3459, 4767
DraIII CACNNNGTG 1 cut(s) 4736
EaeI YGGCCR 5 cut(s) 3, 2996, 3446, 5025, 5222
Eam1104I CTCTTC 6 cut(s) 1404, 2685, 2928, 3788, 3834, 6072
EarI CTCTTC 6 cut(s) 1404, 2685, 2928, 3788, 3834, 6072
EciI GGCGGA 2 cut(s) 229, 4408
Ecl136II GAGCTC 2 cut(s) 193, 3781
Eco105I TACGTA 1 cut(s) 5714
Eco130I CCWWGG 3 cut(s) 209, 694, 2299
Eco147I AGGCCT 1 cut(s) 3812
Eco24I GRGCYC 4 cut(s) 195, 865, 3783, 5691
Eco31I GGTCTC 2 cut(s) 941, 5278
Eco32I GATATC 1 cut(s) 297
Eco47I GGWCC 7 cut(s) 206, 1445, 2317, 2634, 5572, 6107, 6281
Eco53kI GAGCTC 2 cut(s) 193, 3781
Eco72I CACGTG 1 cut(s) 1651
EcoICRI GAGCTC 2 cut(s) 193, 3781
EcoO109I RGGNCCY 5 cut(s) 861, 1445, 2317, 4327, 6281
EcoRV GATATC 1 cut(s) 297
EcoT14I CCWWGG 3 cut(s) 209, 694, 2299
EcoT22I ATGCAT 5 cut(s) 1512, 2763, 3757, 3895, 5117
EcoT38I GRGCYC 4 cut(s) 195, 865, 3783, 5691
ErhI CCWWGG 3 cut(s) 209, 694, 2299
Esp3I CGTCTC 1 cut(s) 4777
FalI AAGNNNNNCTT 4 cut(s) 2205, 2237, 4685, 4717
FaqI GGGAC 2 cut(s) 1458, 1657
FauI CCCGC 1 cut(s) 5340
FauNDI CATATG 3 cut(s) 3651, 4875, 6140
FbaI TGATCA 2 cut(s) 4126, 4369
FblI GTMKAC 2 cut(s) 5716, 6216
FriOI GRGCYC 4 cut(s) 195, 865, 3783, 5691
GlaI GCGC 2 cut(s) 154, 6222
GsaI CCCAGC 2 cut(s) 484, 872
GsuI CTGGAG 6 cut(s) 242, 1569, 2532, 3488, 4641, 6060
HapII CCGG 4 cut(s) 6, 1802, 3536, 3805
HgaI GACGC 2 cut(s) 4794, 4820
HhaI GCGC 2 cut(s) 155, 6223
Hin1I GRCGYC 1 cut(s) 4786
Hin6I GCGC 2 cut(s) 153, 6221
HinP1I GCGC 2 cut(s) 153, 6221
HincII GTYRAC 5 cut(s) 1639, 1868, 2100, 2170, 5863
HindII GTYRAC 5 cut(s) 1639, 1868, 2100, 2170, 5863
HindIII AAGCTT 6 cut(s) 1744, 1986, 2515, 4210, 4900, 5949
HpaI GTTAAC 2 cut(s) 1639, 2100
HpaII CCGG 4 cut(s) 6, 1802, 3536, 3805
Hpy99I CGWCG 1 cut(s) 4484
HpyCH4IV ACGT 6 cut(s) 358, 556, 1650, 5473, 5713, 6256
HpySE526I ACGT 6 cut(s) 358, 556, 1650, 5473, 5713, 6256
Hsp92I GRCGYC 1 cut(s) 4786
HspAI GCGC 2 cut(s) 153, 6221
Kpn2I TCCGGA 1 cut(s) 1801
KroI GCCGGC 1 cut(s) 5
KroNI GCCGGC 1 cut(s) 7
Ksp22I TGATCA 2 cut(s) 4126, 4369
KspAI GTTAAC 2 cut(s) 1639, 2100
LguI GCTCTTC 1 cut(s) 3788
MabI ACCWGGT 2 cut(s) 3137, 6208
MaeII ACGT 6 cut(s) 358, 556, 1650, 5473, 5713, 6256
MfeI CAATTG 1 cut(s) 647
MflI RGATCY 5 cut(s) 384, 721, 4138, 4670, 5611
MlsI TGGCCA 2 cut(s) 5027, 5224
MluNI TGGCCA 2 cut(s) 5027, 5224
MmeI TCCRAC 4 cut(s) 1437, 2068, 2886, 4170
Mox20I TGGCCA 2 cut(s) 5027, 5224
Mph1103I ATGCAT 5 cut(s) 1512, 2763, 3757, 3895, 5117
MroI TCCGGA 1 cut(s) 1801
MroNI GCCGGC 1 cut(s) 5
MroXI GAANNNNTTC 5 cut(s) 1209, 3173, 3720, 5404, 6014
MscI TGGCCA 2 cut(s) 5027, 5224
MslI CAYNNNNRTG 6 cut(s) 1041, 1653, 1935, 4353, 5174, 5916
Msp20I TGGCCA 2 cut(s) 5027, 5224
MspA1I CMGCKG 2 cut(s) 2429, 5423
MspI CCGG 4 cut(s) 6, 1802, 3536, 3805
MunI CAATTG 1 cut(s) 647
Mva1269I GAATGC 4 cut(s) 1183, 3757, 4090, 5927
MvnI CGCG 1 cut(s) 6221
NaeI GCCGGC 1 cut(s) 7
NcoI CCATGG 2 cut(s) 209, 694
NdeI CATATG 3 cut(s) 3651, 4875, 6140
NgoMIV GCCGGC 1 cut(s) 5
NmuCI GTSAC 9 cut(s) 185, 354, 803, 1362, 2398, 3090, 3401, 3538, 3694
NsiI ATGCAT 5 cut(s) 1512, 2763, 3757, 3895, 5117
NspI RCATGY 4 cut(s) 2765, 3872, 5672, 5699
OliI CACNNNNGTG 1 cut(s) 5916
PaeI GCATGC 1 cut(s) 2765
PagI TCATGA 3 cut(s) 3606, 4372, 5152
PceI AGGCCT 1 cut(s) 3812
PciSI GCTCTTC 1 cut(s) 3788
PcsI WCGNNNNNNNCGW 2 cut(s) 75, 1647
PctI GAATGC 4 cut(s) 1183, 3757, 4090, 5927
PdiI GCCGGC 1 cut(s) 7
PdmI GAANNNNTTC 5 cut(s) 1209, 3173, 3720, 5404, 6014
PflFI GACNNNGTC 1 cut(s) 5161
PflMI CCANNNNNTGG 1 cut(s) 2472
PfoI TCCNGGA 1 cut(s) 5614
Ple19I CGATCG 1 cut(s) 38
PmaCI CACGTG 1 cut(s) 1651
PmlI CACGTG 1 cut(s) 1651
Ppu21I YACGTR 2 cut(s) 1651, 5714
PpuMI RGGWCCY 3 cut(s) 1445, 2317, 6281
PshBI ATTAAT 3 cut(s) 105, 963, 1850
PsiI TTATAA 2 cut(s) 1581, 4242
Psp124BI GAGCTC 2 cut(s) 195, 3783
Psp1406I AACGTT 1 cut(s) 5473
Psp5II RGGWCCY 3 cut(s) 1445, 2317, 6281
PspCI CACGTG 1 cut(s) 1651
PspFI CCCAGC 2 cut(s) 480, 868
PspOMI GGGCCC 1 cut(s) 861
PspPPI RGGWCCY 3 cut(s) 1445, 2317, 6281
PsrI GAACNNNNNNTAC 2 cut(s) 1522, 1554
PstI CTGCAG 1 cut(s) 3344
PstNI CAGNNNCTG 1 cut(s) 4673
PsuI RGATCY 5 cut(s) 384, 721, 4138, 4670, 5611
PsyI GACNNNGTC 1 cut(s) 5161
PvuI CGATCG 1 cut(s) 38
PvuII CAGCTG 2 cut(s) 2429, 5423
RsaI GTAC 8 cut(s) 653, 1306, 1383, 2331, 3028, 5646, 5678, 5964
RsaNI GTAC 8 cut(s) 652, 1305, 1382, 2330, 3027, 5645, 5677, 5963
RseI CAYNNNNRTG 6 cut(s) 1041, 1653, 1935, 4353, 5174, 5916
SacI GAGCTC 2 cut(s) 195, 3783
SapI GCTCTTC 1 cut(s) 3788
ScaI AGTACT 2 cut(s) 1306, 2331
SexAI ACCWGGT 2 cut(s) 3137, 6208
SfcI CTRYAG 6 cut(s) 738, 1861, 3340, 3525, 5090, 5322
SinI GGWCC 7 cut(s) 206, 1445, 2317, 2634, 5572, 6107, 6281
SmiMI CAYNNNNRTG 6 cut(s) 1041, 1653, 1935, 4353, 5174, 5916
SmlI CTYRAG 7 cut(s) 260, 339, 911, 1121, 1406, 3884, 4897
SmoI CTYRAG 7 cut(s) 260, 339, 911, 1121, 1406, 3884, 4897
SnaBI TACGTA 1 cut(s) 5714
SpeI ACTAGT 3 cut(s) 1301, 2254, 5335
SphI GCATGC 1 cut(s) 2765
SseBI AGGCCT 1 cut(s) 3812
SspI AATATT 3 cut(s) 3130, 4522, 4591
SstI GAGCTC 2 cut(s) 195, 3783
StuI AGGCCT 1 cut(s) 3812
StyI CCWWGG 3 cut(s) 209, 694, 2299
TaiI ACGT 6 cut(s) 361, 559, 1653, 5476, 5716, 6259
TatI WGTACW 7 cut(s) 651, 1304, 1381, 2329, 3026, 5644, 5962
TauI GCSGC 3 cut(s) 321, 2942, 3735
TseFI GTSAC 9 cut(s) 185, 354, 803, 1362, 2398, 3090, 3401, 3538, 3694
Tsp45I GTSAC 9 cut(s) 185, 354, 803, 1362, 2398, 3090, 3401, 3538, 3694
TspGWI ACGGA 5 cut(s) 559, 813, 1547, 4211, 4673
Tth111I GACNNNGTC 1 cut(s) 5161
Van91I CCANNNNNTGG 1 cut(s) 2472
VneI GTGCAC 3 cut(s) 3142, 4728, 5356
VpaK11BI GGWCC 7 cut(s) 206, 1445, 2317, 2634, 5572, 6107, 6281
VspI ATTAAT 3 cut(s) 105, 963, 1850
XbaI TCTAGA 2 cut(s) 299, 2122
XceI RCATGY 4 cut(s) 2765, 3872, 5672, 5699
XcmI CCANNNNNNNNNTGG 1 cut(s) 3105
XmiI GTMKAC 2 cut(s) 5716, 6216
XmnI GAANNNNTTC 5 cut(s) 1209, 3173, 3720, 5404, 6014
ZrmI AGTACT 2 cut(s) 1306, 2331
Zsp2I ATGCAT 5 cut(s) 1512, 2763, 3757, 3895, 5117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.