Rmu_sc0001925.1_g000002

Urb2/Npa2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001925.1
OG0000376
Physical Location & Seq
Forward (+)
7854 .. 15393
7540 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001925.1_g000002.1.cds

Sequence Viewer

Length: 5916 bp
atggccgattctggagctaaccctaaacagagacaacccaagaagaacaagaagaagagaaagcagagcggaccccaagaatctgaaaggccacccaagactaaccgcaccaattcatcagagaacgagaatgaaccacaagaccaaaactttgaagttggaaaagctgaaaaacccaatcaaggtggtccatggaggaacctggagttaattctgtcaatccagaacagagagcttgatcttcacaagaaggtggaactagctcatggttttgtgatgtcgagggtgaaggaagaaggaggcagcagtgatgaggataatcaagcagtgaacatgtcacgtttagtaatttttgtcagtgattggatccagtcattgttgatttctgaaggaaataaagttgagagtggtggagagaagcatcaagctgaagttatttatacgtatttggatttaagatgctgggagattttcaaattctgtttggaggagtctctgaaattaaatgttttgttaagtttctcacggaaccttttacgctctatatgttggattgcaaggagcgcactgtccttattgaacaaaaaaattattggtgaagggttacaattgtacactactgtgcttgattgtatttctttggtattttcatcccaagaaggcttctcaaatgaaaatttagacatgtggattataactgtaagttcagttcttgaccttggacacaaattttacagtgaagctcctgttggtggcaatgagggtgtatttgttttccgattcttgtgcttggtgcttgagccatttgctaggtttttaagggctcacgcagctcggaaagatggatttcgtgattttagtaataagctccttgagcctctgttgcacctcttgggtctcttgcatctccagattggtggaagtaatcctggttggagaagaaacttactgaagcttgttgaagatgtattatctcatgggctttatcacccgctccacattgatgggtttttgagcttatgcagtactgaaaaagacaccacatccaatgagaggactgtgtcagttgtaagagatgtgaatccgcctgtccccgaatccggctgctggtgcgggcttatacctggctggatgggactttctaaatgcgggacgaaatccggaagccaattgctggctctgaataaccagccgttcaattcaattcttcggaagtcaaaagattccaaaacaatcaaccagagttatcatagacatttatttgataaagtggaaggaattttagctgcaaagaatgcatttgcagtagaaagcataggagtattgtttcacttgcttattgatcaagttaaaaagctaaagagggattcagcgctgactgccagtaccaaaatgatggggaagagtgaaggttcaaggcagttagaagattgttcattgggtcaaacgtctatgatgtcttctgagagtcgtattgttgaaaagaattattgctcaactagtttcaatccaggaacacgaaagtcacttcacgatttttttgtactgattatggagcctcttttgcttgagatcaatggctttcttgaaactaaactgaaagtgggccctgaattgttggatattcattgcacacttaaagctataaataatgtactttcggtctttatgcatgaaaagatttacgtgagaacagaggatgcctctgagggagcttctcttaatttcttgaagaaggtttataatattatcatctcattgtcctcagatttaattcagttgtcagttacacacttagatagttttactctaatagcaagtgaggtactttctgctgttggctatcttttggaaattgagtatgatgttctggagaatgatctagtaagcttgtggttaatgatcctctcttactgggccattgggctttctttggtggattctccggatagatgcacattattctcgaagataacagctattggatgccaattaattatgctctatagtcaacttcgccaggtgaataatactatctttgcattgtgtaaagcaataagggttattaatttacataatagtgatggtgaactgaactatactagatttgtcattccttttcatggtgaagaatatgcaaaagctgtggaaatgctattatctgcacatgaattcaaaattgcaatccataaagcaatgaagtctataccagaaggacaagcaagtcggtgtattcggcagttaacattggatatatcagaatctgtagagtggatgaaagttagttgttcggaggctgatgaaaaagaattctctgaatgccatctaagtagcttgcacagttttaatctggaagctgaacttttcgggagagggttgtcagaaatatatgcattattcctagagtcattgattgtcacaaccggaaactgtaaccttcttggcgcttctataaaggaattaattagagtgattttcccttgcatgagcaatctggttggaccacagcaggaggatgctgtaaacaaattcctatggtccgtcgcggtaaaagattctgacaatgtgatttctagaaacaaaaacaaatatctgatatttggagtctccacttattgggtagttttgttcttctttcgattgtatacctccagccgatgcttatataggcaagcagcaatccttatgcctccagatttatcaagaaagatgtctgcagaaatgggagattcgttctcctcattctctggcagggattggatggaaatgtctgattggatcaatgggggatttttctcttggattgtacaaccttcagcttctcttctttctgttattcaatctgtttcaaacatcttttgtaaggaccgtgcagctgattcttgtcctttaacttatgtaatgcatgctattgcttgtgaaaggcttgtggatttgaatagtcgtataaagtcttttgaatatttgattgaaaatggtgacaatctggtgcaaattgcggagatctcttctttgaggcaagaggcagcaggactcacgggattcatgatggagcacctctcattagtgtctgaagatcaacagcgaatattcatttctgctgatacgactaataataagatggttaactatgaaagtgatgaatgggatttcagtatttgctctgtgaacaagaagtcattgccaactgcactttggtgggttgtttgccaaaatattcatgcatggtgccctcatgcctctaaaaaggatttgaagagattcctttctcttttaatccatacttcaattccctatgtaagaagcagctttggggtggttatagagcacaagaaccatgaagctgacaggctgaagaagaactcatcattggctatttcctgcaacaaactcaatgtatttgattgttcctcagcctcaagttgcacgggacaaaactcccaaccatctaccatcatgaaatttactgcttgtcagagtttgcttaatcttttgagttgtatgccaaaaggtcatttggacacgagatcattttcacgttatgtgacttctattctgaacctcgaaaggattgttgttggcggcttattagattatcagaatgcttcatactcaacttattattatgagctcttcagattgtttgtgtcctgtcggaaggccttaagatgtataattactgcttgtgaggagacagtagccagtcgaacctcagacaccgtactctttgaggatttatttccagtttcatggctttacaagtcagtgcatatggttgctgggcttcaagaatcattctcaaaagatatttatcatcaagtccatgatatgatcttggcgttaatggatcacacattttatgtgtttctgacattaaataaatatcaatccaatcatgctgttcgtttccttgaggttgctgagctgaattttggatgtgcccaagaacagagaagtttattgaattcgtccaattattttgaagcctggaaaagtgtaaacattgttgctaagatgttgaaggaacagatgcagatcttacttgtaaatgtgaagaatggcatttgtaatggaaaagaggaagtttctgttgatgctttaaatttgaacaagtttgcttccataatttcgtgttttagtgggtttttatggggcctagcttgcgttgtgatcgacacagatgggagaaatagtgatgagaaagctaagttgtcgaggtggaaacttgaaccaatctccgaactcaacctctgtataaaggtttttgaagaaatttctagtcttctactacaaatgtttattcttgacgataatcagcagtccacagctatatgtcatgcctataatcttcagaagtcaggctacaatgtagatttgttgggtgcagagaagatttttccggatggcaatgatactgacactgatatggcatgtggtggactgcaggatgaatctgcagttgcagtgacgtgctcagcatcatctgacatctgtgatgactctgtaattggtagtgttcatagaagaagaccgcgcttgaaagatgcaaatagtgttgttagtgttctcactgctgttgattcatttgaactgcaatttttaaataagcctttgtttagaagcatgctaaaaggtgatttccccaacgcagcatttttactcaggcagttgttaatcgcctcttctgctattttgaggctaaatttgcatattaagagtgctcccatgttgtcaagcttggtgcataagtttgctggcattatgcaagtcttgttattggaatcagcggacccaattcaggtgccacatgtctactattttgtgtgtttagatggtgttctgaagtatctggaagaattagggaatcattttcctttgtctaatcctgcctcatccagagatttgtttgacaagatggttaagctacagttacgggccctagggaaatgcataactttacaagggaaaagagcaactcttgcatctcatgaaacaagcaccaacacacccataggagaaaggagattttctgaagcatcatctttttctggctgggaatatttattggatgaattcaaagctaggttgaggtcatcattcgccgtgttcataaagaaatcaacagagttgcatctccaatctgcagttcaagctatcgaaagagccctagttggtgtgcgggaagggtgcacagtgaggtatgacatatgtgctggaaatgaagatggaggaaaggtttcttcaattgttgcatctggcattgattgcttggatttggttctggaatttgtttcaggacgcaatttgattgtggttaaaaaatacattcagaggttaattgcctgcatgttcaatgttattctgcatctgcagagtccactaatcttctacgagagattcactcagagtaaagatgatagtaatccggatccaggaacagtcattcttatgtgtgttgacgtactggcaagaatttctggcaagcatgcaatatacaaaatggatctctggcatgtagcacacttgttacgtataccttcagcactttttcaagatttccatctgttaaaagattctaaacgtcctgttccaaatgattcttcagcaagtctaaataaccaaccatccgacccagtagcaagtgtgcatgtctctggtatagataggcaatactcaatcggcctatattctgcttgctgccgattattgcataatgttgtgaagcatcacaaaagtgaatgcgaggggtatgttgctctacttcaagcttctgttcgtgttcttctttattgtttggagacattggatgctgttgtcgtagccagggaaggcttcttttcatgggaagtagaagaaggagtcaaatgcgcatgttttctcagaaggatttatgaagaggtaggtgctcacaggtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Urb2

Protein Analysis

1971

Amino Acids

221.39

Weight (kDa)

6.41

Isoelectric Point (pI)

47.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000376)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30150 AT4G30150
fragaria_vesca FvH4_2g02080 FvH4_2g02080
malus_domestica MD05G1115300.v1.1 MD10G1117200.v1.1 MD10G1117300.v1.1 MD10G1117400.v1.1 MD10G1117500.v1.1 MD10G1117600.v1.1 MD10G1117700.v1.1
prunus_persica Prupe.8G159900_v2.0.a1 Prupe.8G159900_v2.0.a1 Prupe.8G159900_v2.0.a1
pyrus_communis pycom05g10980 pycom10g10170
rosa_chinensis RchiOBHm_Chr2g0118221 RchiOBHm_Chr6g0244891 RchiOBHm_Chr6g0244901 RchiOBHm_Chr6g0244911 RchiOBHm_Chr6g0244921 RchiOBHm_Chr6g0244931 RchiOBHm_Chr6g0244961 RchiOBHm_Chr6g0244971 RchiOBHm_Chr6g0245301 RchiOBHm_Chr6g0245311 RchiOBHm_Chr6g0245351 RchiOBHm_Chr6g0245361 RchiOBHm_Chr6g0245441 RchiOBHm_Chr6g0245521 RchiOBHm_Chr6g0245531 RchiOBHm_Chr6g0245571
rosa_laevigata RLG00000015303
rosa_multiflora Rmu_sc0000471.1_g000006 Rmu_sc0000471.1_g000007 Rmu_sc0000471.1_g000008 Rmu_sc0001288.1_g000012 Rmu_sc0001925.1_g000002 Rmu_sc0002202.1_g000009 Rmu_sc0002202.1_g000016 Rmu_sc0002202.1_g000017 Rmu_sc0002202.1_g000019 Rmu_sc0002202.1_g000020 Rmu_ssc0000087.1_g000055 Rmu_ssc0000087.1_g000056
rosa_roxburghii Rroxscaffold_3G00242140 Rroxscaffold_7G00215270
rosa_rugosa Rorug05G0511800 Rorug05G0513500 Rorug05G0513900 Rorug05G0514000 Rorug05G0514200 Rorug05G0514400
rosa_samantha Rh1AG142300 Rh1DG147200 Rh6AG026300 Rh6AG026400 Rh6AG026500 Rh6AG028900 Rh6AG029000 Rh6AG029100 Rh6AG029200 Rh6AG029300 Rh6AG029400 Rh6AG029500 Rh6BG025200 Rh6CG020200 Rh6CG020300 Rh6CG020400 Rh6CG023100 Rh6CG023300 Rh6CG023500 Rh6CG023800 Rh6CG024100 Rh6CG024200 Rh6CG024400 Rh6CG024700 Rh6CG024800 Rh6CG025200 Rh6CG025400 Rh6CG025600 Rh6CG025700 Rh6CG025800 Rh6CG025900 Rh6CG026000 Rh6DG020800 Rh6DG020900 Rh6DG021000 Rh6DG023100 Rh6DG023300 Rh6DG023500 Rh6DG023700 Rh6DG023900 Rh6DG024200 Rh6DG024500 Rh6DG024700 Rh6DG024900 Rh6DG025000 Rh6DG025200 Rh6DG025300 Rh6DG025400
rosa_wichuraiana Rw2G021630 Rw2G021750 Rw6G002430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 695, 1743
AasI GACNNNNNNGTC 1 cut(s) 2223
Acc16I TGCGCA 1 cut(s) 5869
AccB1I GGYRCC 2 cut(s) 3228, 4801
AccB7I CCANNNNNTGG 2 cut(s) 1174, 2616
AccBSI CCGCTC 2 cut(s) 69, 994
AccI GTMKAC 3 cut(s) 2645, 4812, 5533
AccII CGCG 2 cut(s) 2546, 4534
AccIII TCCGGA 4 cut(s) 1160, 1945, 4399, 5425
AclWI GGATC 8 cut(s) 361, 374, 1897, 2786, 3855, 5423, 5436, 5511
AcoI YGGCCR 1 cut(s) 3
AfaI GTAC 9 cut(s) 614, 1027, 1387, 1545, 1656, 1827, 2808, 3723, 5463
AfeI AGCGCT 1 cut(s) 1374
AflII CTTAAG 1 cut(s) 3664
AflIII ACRYGT 3 cut(s) 333, 684, 4807
AhlI ACTAGT 1 cut(s) 1499
AjiI CACGTC 1 cut(s) 4470
AjnI CCWGG 8 cut(s) 201, 928, 1123, 1510, 2019, 3986, 5431, 5822
AjuI GAANNNNNNNTTGG 8 cut(s) 732, 764, 3353, 3385, 5130, 5162, 5584, 5616
AleI CACNNNNGTG 3 cut(s) 620, 3196, 5733
Alw21I GWGCWC 7 cut(s) 3057, 3330, 3633, 4475, 4723, 5192, 5908
Alw26I GTCTC 7 cut(s) 25, 498, 902, 2611, 3686, 5656, 5792
Alw44I GTGCAC 1 cut(s) 5188
AlwI GGATC 8 cut(s) 361, 374, 1897, 2786, 3855, 5423, 5436, 5511
AlwNI CAGNNNCTG 1 cut(s) 2264
Aor13HI TCCGGA 4 cut(s) 1160, 1945, 4399, 5425
Aor51HI AGCGCT 1 cut(s) 1374
AoxI GGCC 8 cut(s) 3, 89, 1606, 1917, 3660, 4153, 4935, 5680
ApaI GGGCCC 2 cut(s) 1610, 4939
ApaLI GTGCAC 1 cut(s) 5188
ArsI GACNNNNNNTTYG 2 cut(s) 134, 166
AseI ATTAAT 3 cut(s) 1994, 2067, 2462
Asp700I GAANNNNTTC 2 cut(s) 3260, 5236
AspA2I CCTAGG 1 cut(s) 4939
AspLEI GCGC 5 cut(s) 566, 1375, 2447, 4536, 5870
AsuHPI GGTGA 8 cut(s) 298, 608, 980, 2035, 2099, 2138, 2990, 4646
AvaII GGWCC 6 cut(s) 71, 188, 2501, 2538, 2866, 4789
AvrII CCTAGG 1 cut(s) 4939
BaeGI GKGCMC 5 cut(s) 1610, 3233, 3943, 4939, 5192
BamHI GGATCC 2 cut(s) 366, 5428
BanI GGYRCC 2 cut(s) 3228, 4801
BanII GRGCYC 5 cut(s) 826, 1610, 3633, 4939, 5167
BarI GAAGNNNNNNTAC 2 cut(s) 4346, 4378
BauI CACGAG 1 cut(s) 3523
BbsI GAAGAC 3 cut(s) 1452, 4274, 4534
Bbv12I GWGCWC 7 cut(s) 3057, 3330, 3633, 4475, 4723, 5192, 5908
BbvCI CCTCAGC 1 cut(s) 3412
BceAI ACGGC 2 cut(s) 1177, 5087
BcgI CGANNNNNNTGC 2 cut(s) 768, 802
BciT130I CCWGG 8 cut(s) 203, 930, 1125, 1512, 2021, 3988, 5433, 5824
BclI TGATCA 1 cut(s) 1342
BcoDI GTCTC 7 cut(s) 25, 498, 902, 2611, 3686, 5656, 5792
BcuI ACTAGT 1 cut(s) 1499
BfmI CTRYAG 8 cut(s) 2005, 2265, 2715, 4442, 4455, 4925, 5142, 5369
BfoI RGCGCY 2 cut(s) 1376, 2448
BfrI CTTAAG 1 cut(s) 3664
BglII AGATCT 2 cut(s) 3003, 4035
BlnI CCTAGG 1 cut(s) 4939
BlpI GCTNAGC 2 cut(s) 3921, 4474
BmcAI AGTACT 1 cut(s) 1027
Bme1390I CCNGG 8 cut(s) 203, 930, 1125, 1512, 2021, 3988, 5433, 5824
Bme18I GGWCC 6 cut(s) 71, 188, 2501, 2538, 2866, 4789
BmgBI CACGTC 1 cut(s) 4470
BmrFI CCNGG 8 cut(s) 203, 930, 1125, 1512, 2021, 3988, 5433, 5824
BmrI ACTGGG 2 cut(s) 1924, 5627
BmuI ACTGGG 2 cut(s) 1924, 5627
BpiI GAAGAC 3 cut(s) 1452, 4274, 4534
BplI GAGNNNNNCTC 6 cut(s) 1688, 1720, 3044, 3076, 5386, 5418
BpmI CTGGAG 6 cut(s) 33, 224, 893, 1892, 2635, 2676
Bpu10I CCTNAGC 1 cut(s) 3412
Bpu1102I GCTNAGC 2 cut(s) 3921, 4474
BpuEI CTTGAG 5 cut(s) 818, 893, 1589, 3403, 3932
BsaAI YACGTR 3 cut(s) 444, 1687, 5531
BsaBI GATNNNNATC 5 cut(s) 1080, 3810, 4034, 5421, 5559
BsaI GGTCTC 1 cut(s) 902
BsaJI CCNNGG 4 cut(s) 191, 718, 4939, 5823
BsaWI WCCGGW 5 cut(s) 1160, 1945, 2423, 4399, 5425
BsaXI ACNNNNNCTCC 4 cut(s) 4177, 4207, 5220, 5250
Bse1I ACTGG 7 cut(s) 370, 1383, 1919, 3702, 3743, 5469, 5633
Bse3DI GCAATG 5 cut(s) 763, 1627, 2202, 3179, 4414
Bse8I GATNNNNATC 5 cut(s) 1080, 3810, 4034, 5421, 5559
BseAI TCCGGA 4 cut(s) 1160, 1945, 4399, 5425
BseBI CCWGG 8 cut(s) 203, 930, 1125, 1512, 2021, 3988, 5433, 5824
BseDI CCNNGG 4 cut(s) 191, 718, 4939, 5823
BseJI GATNNNNATC 5 cut(s) 1080, 3810, 4034, 5421, 5559
BseMI GCAATG 5 cut(s) 763, 1627, 2202, 3179, 4414
BseNI ACTGG 7 cut(s) 370, 1383, 1919, 3702, 3743, 5469, 5633
BseRI GAGGAG 3 cut(s) 503, 2728, 3704
BseSI GKGCMC 5 cut(s) 1610, 3233, 3943, 4939, 5192
BseYI CCCAGC 3 cut(s) 462, 3779, 5052
BsgI GTGCAG 4 cut(s) 2148, 2892, 3174, 4404
Bsh1236I CGCG 2 cut(s) 2546, 4534
BshFI GGCC 8 cut(s) 5, 91, 1608, 1919, 3662, 4155, 4937, 5682
BshNI GGYRCC 2 cut(s) 3228, 4801
BsiHKAI GWGCWC 7 cut(s) 3057, 3330, 3633, 4475, 4723, 5192, 5908
BsiSI CCGG 6 cut(s) 1101, 1161, 1946, 2424, 4400, 5426
BslFI GGGAC 4 cut(s) 1076, 1149, 1165, 3444
BsmAI GTCTC 7 cut(s) 25, 498, 902, 2611, 3686, 5656, 5792
BsmFI GGGAC 4 cut(s) 1076, 1149, 1165, 3444
BsmI GAATGC 4 cut(s) 1300, 2324, 3607, 5744
BsnI GGCC 8 cut(s) 5, 91, 1608, 1919, 3662, 4155, 4937, 5682
Bso31I GGTCTC 1 cut(s) 902
Bsp120I GGGCCC 2 cut(s) 1606, 4935
Bsp13I TCCGGA 4 cut(s) 1160, 1945, 4399, 5425
Bsp1407I TGTACA 2 cut(s) 612, 2806
Bsp1720I GCTNAGC 2 cut(s) 3921, 4474
Bsp19I CCATGG 1 cut(s) 191
BspANI GGCC 8 cut(s) 5, 91, 1608, 1919, 3662, 4155, 4937, 5682
BspEI TCCGGA 4 cut(s) 1160, 1945, 4399, 5425
BspFNI CGCG 2 cut(s) 2546, 4534
BspHI TCATGA 3 cut(s) 3045, 3456, 4987
BspMAI CTGCAG 5 cut(s) 2719, 4446, 4459, 5146, 5373
BspPI GGATC 8 cut(s) 361, 374, 1897, 2786, 3855, 5423, 5436, 5511
BspQI GCTCTTC 1 cut(s) 3638
BspT107I GGYRCC 2 cut(s) 3228, 4801
BspTI CTTAAG 1 cut(s) 3664
BspTNI GGTCTC 1 cut(s) 902
BsrBI CCGCTC 2 cut(s) 69, 994
BsrDI GCAATG 5 cut(s) 763, 1627, 2202, 3179, 4414
BsrGI TGTACA 2 cut(s) 612, 2806
BsrI ACTGG 7 cut(s) 370, 1383, 1919, 3702, 3743, 5469, 5633
BssECI CCNNGG 4 cut(s) 191, 718, 4939, 5823
BssNAI GTATAC 2 cut(s) 2646, 5534
BssSI CACGAG 1 cut(s) 3523
BssT1I CCWWGG 3 cut(s) 191, 718, 4939
Bst1107I GTATAC 2 cut(s) 2646, 5534
Bst2BI CACGAG 1 cut(s) 3523
Bst2UI CCWGG 8 cut(s) 203, 930, 1125, 1512, 2021, 3988, 5433, 5824
Bst6I CTCTTC 8 cut(s) 50, 1397, 2829, 3013, 3251, 3638, 4687, 5889
BstAFI CTTAAG 1 cut(s) 3664
BstAPI GCANNNNNTGC 3 cut(s) 1295, 4979, 5265
BstAUI TGTACA 2 cut(s) 612, 2806
BstBAI YACGTR 3 cut(s) 444, 1687, 5531
BstDSI CCRYGG 1 cut(s) 191
BstFNI CGCG 2 cut(s) 2546, 4534
BstH2I RGCGCY 2 cut(s) 1376, 2448
BstHHI GCGC 5 cut(s) 566, 1375, 2447, 4536, 5870
BstMAI GTCTC 7 cut(s) 25, 498, 902, 2611, 3686, 5656, 5792
BstNI CCWGG 8 cut(s) 203, 930, 1125, 1512, 2021, 3988, 5433, 5824
BstSCI CCNGG 8 cut(s) 201, 928, 1123, 1510, 2019, 3986, 5431, 5822
BstSFI CTRYAG 8 cut(s) 2005, 2265, 2715, 4442, 4455, 4925, 5142, 5369
BstSLI GKGCMC 5 cut(s) 1610, 3233, 3943, 4939, 5192
BstSNI TACGTA 2 cut(s) 444, 5531
BstUI CGCG 2 cut(s) 2546, 4534
BstV2I GAAGAC 3 cut(s) 1452, 4274, 4534
BstX2I RGATCY 5 cut(s) 366, 3003, 4035, 5428, 5503
BstXI CCANNNNNNTGG 4 cut(s) 917, 1004, 1396, 3750
BstYI RGATCY 5 cut(s) 366, 3003, 4035, 5428, 5503
BstZ17I GTATAC 2 cut(s) 2646, 5534
BsuRI GGCC 8 cut(s) 5, 91, 1608, 1919, 3662, 4155, 4937, 5682
BtgI CCRYGG 1 cut(s) 191
BtrI CACGTC 1 cut(s) 4470
BtsI GCAGTG 4 cut(s) 313, 333, 4470, 4569
CaiI CAGNNNCTG 1 cut(s) 2264
CciI TCATGA 3 cut(s) 3045, 3456, 4987
CfoI GCGC 5 cut(s) 566, 1375, 2447, 4536, 5870
CseI GACGC 1 cut(s) 5307
Csp6I GTAC 9 cut(s) 613, 1026, 1386, 1544, 1655, 1826, 2807, 3722, 5462
CspCI CAANNNNNGTGG 4 cut(s) 166, 201, 2127, 2162
CviQI GTAC 9 cut(s) 613, 1026, 1386, 1544, 1655, 1826, 2807, 3722, 5462
DraI TTTAAA 2 cut(s) 4101, 4602
DrdI GACNNNNNNGTC 1 cut(s) 2223
DseDI GACNNNNNNGTC 1 cut(s) 2223
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 8 cut(s) 50, 1397, 2829, 3013, 3251, 3638, 4687, 5889
EarI CTCTTC 8 cut(s) 50, 1397, 2829, 3013, 3251, 3638, 4687, 5889
EciI GGCGGA 1 cut(s) 1074
Ecl136II GAGCTC 1 cut(s) 3631
Eco105I TACGTA 2 cut(s) 444, 5531
Eco130I CCWWGG 3 cut(s) 191, 718, 4939
Eco147I AGGCCT 1 cut(s) 3662
Eco24I GRGCYC 5 cut(s) 826, 1610, 3633, 4939, 5167
Eco31I GGTCTC 1 cut(s) 902
Eco47I GGWCC 6 cut(s) 71, 188, 2501, 2538, 2866, 4789
Eco47III AGCGCT 1 cut(s) 1374
Eco53kI GAGCTC 1 cut(s) 3631
EcoICRI GAGCTC 1 cut(s) 3631
EcoO109I RGGNCCY 3 cut(s) 1607, 4153, 4936
EcoRI GAATTC 4 cut(s) 2171, 2309, 3964, 5072
EcoRII CCWGG 8 cut(s) 201, 928, 1123, 1510, 2019, 3986, 5431, 5822
EcoT14I CCWWGG 3 cut(s) 191, 718, 4939
EcoT22I ATGCAT 6 cut(s) 1300, 1674, 2395, 2907, 3226, 4952
EcoT38I GRGCYC 5 cut(s) 826, 1610, 3633, 4939, 5167
ErhI CCWWGG 3 cut(s) 191, 718, 4939
FalI AAGNNNNNCTT 2 cut(s) 2346, 2378
FaqI GGGAC 4 cut(s) 1076, 1149, 1165, 3444
FauI CCCGC 4 cut(s) 999, 1106, 1142, 5172
FauNDI CATATG 2 cut(s) 3771, 5207
FbaI TGATCA 1 cut(s) 1342
FblI GTMKAC 3 cut(s) 2645, 4812, 5533
FriOI GRGCYC 5 cut(s) 826, 1610, 3633, 4939, 5167
FspAI RTGCGCAY 1 cut(s) 5869
FspI TGCGCA 1 cut(s) 5869
GlaI GCGC 5 cut(s) 565, 1374, 2446, 4535, 5869
GsaI CCCAGC 3 cut(s) 466, 3783, 5056
GsuI CTGGAG 6 cut(s) 33, 224, 893, 1892, 2635, 2676
HaeII RGCGCY 2 cut(s) 1376, 2448
HaeIII GGCC 8 cut(s) 5, 91, 1608, 1919, 3662, 4155, 4937, 5682
HapII CCGG 6 cut(s) 1101, 1161, 1946, 2424, 4400, 5426
HgaI GACGC 1 cut(s) 5307
HhaI GCGC 5 cut(s) 566, 1375, 2447, 4536, 5870
Hin6I GCGC 5 cut(s) 564, 1373, 2445, 4534, 5868
HinP1I GCGC 5 cut(s) 564, 1373, 2445, 4534, 5868
HincII GTYRAC 4 cut(s) 2012, 2244, 3127, 5458
HindII GTYRAC 4 cut(s) 2012, 2244, 3127, 5458
HindIII AAGCTT 4 cut(s) 953, 1888, 4735, 5766
HpaI GTTAAC 2 cut(s) 2244, 3127
HpaII CCGG 6 cut(s) 1101, 1161, 1946, 2424, 4400, 5426
HphI GGTGA 8 cut(s) 298, 608, 980, 2035, 2099, 2138, 2990, 4646
Hpy99I CGWCG 1 cut(s) 2546
HpyCH4IV ACGT 9 cut(s) 340, 443, 1448, 1686, 3538, 4469, 5460, 5530, 5581
HpySE526I ACGT 9 cut(s) 340, 443, 1448, 1686, 3538, 4469, 5460, 5530, 5581
HspAI GCGC 5 cut(s) 564, 1373, 2445, 4534, 5868
Kpn2I TCCGGA 4 cut(s) 1160, 1945, 4399, 5425
Ksp22I TGATCA 1 cut(s) 1342
KspAI GTTAAC 2 cut(s) 2244, 3127
LguI GCTCTTC 1 cut(s) 3638
LmnI GCTCC 9 cut(s) 14, 561, 748, 873, 999, 1555, 1712, 3052, 4726
MaeII ACGT 9 cut(s) 340, 443, 1448, 1686, 3538, 4469, 5460, 5530, 5581
MbiI CCGCTC 2 cut(s) 69, 994
MfeI CAATTG 3 cut(s) 608, 1169, 5244
MflI RGATCY 5 cut(s) 366, 3003, 4035, 5428, 5503
MlyI GAGTC 8 cut(s) 500, 1477, 2414, 2613, 3027, 4493, 5383, 5868
MmeI TCCRAC 7 cut(s) 139, 530, 914, 1599, 2479, 3635, 5652
Mph1103I ATGCAT 6 cut(s) 1300, 1674, 2395, 2907, 3226, 4952
MroI TCCGGA 4 cut(s) 1160, 1945, 4399, 5425
MroXI GAANNNNTTC 2 cut(s) 3260, 5236
MslI CAYNNNNRTG 7 cut(s) 620, 1002, 2079, 3196, 4424, 5447, 5733
MspA1I CMGCKG 2 cut(s) 2876, 4787
MspCI CTTAAG 1 cut(s) 3664
MspI CCGG 6 cut(s) 1101, 1161, 1946, 2424, 4400, 5426
MspR9I CCNGG 8 cut(s) 203, 930, 1125, 1512, 2021, 3988, 5433, 5824
MunI CAATTG 3 cut(s) 608, 1169, 5244
Mva1269I GAATGC 4 cut(s) 1300, 2324, 3607, 5744
MvaI CCWGG 8 cut(s) 203, 930, 1125, 1512, 2021, 3988, 5433, 5824
MvnI CGCG 2 cut(s) 2546, 4534
NcoI CCATGG 1 cut(s) 191
NdeI CATATG 2 cut(s) 3771, 5207
NmuCI GTSAC 6 cut(s) 336, 1524, 2416, 2978, 3544, 4465
NsbI TGCGCA 1 cut(s) 5869
NsiI ATGCAT 6 cut(s) 1300, 1674, 2395, 2907, 3226, 4952
OliI CACNNNNGTG 3 cut(s) 620, 3196, 5733
PaeI GCATGC 3 cut(s) 2909, 4627, 5489
PagI TCATGA 3 cut(s) 3045, 3456, 4987
PceI AGGCCT 1 cut(s) 3662
PciI ACATGT 3 cut(s) 333, 684, 4807
PciSI GCTCTTC 1 cut(s) 3638
PctI GAATGC 4 cut(s) 1300, 2324, 3607, 5744
PdmI GAANNNNTTC 2 cut(s) 3260, 5236
PflFI GACNNNGTC 1 cut(s) 1060
PflMI CCANNNNNTGG 2 cut(s) 1174, 2616
PfoI TCCNGGA 2 cut(s) 1510, 5431
PleI GAGTC 8 cut(s) 499, 1476, 2413, 2612, 3027, 4493, 5382, 5867
PpsI GAGTC 8 cut(s) 499, 1476, 2413, 2612, 3027, 4493, 5382, 5867
Ppu21I YACGTR 3 cut(s) 444, 1687, 5531
PscI ACATGT 3 cut(s) 333, 684, 4807
PshBI ATTAAT 3 cut(s) 1994, 2067, 2462
PsiI TTATAA 2 cut(s) 695, 1743
Psp124BI GAGCTC 1 cut(s) 3633
Psp6I CCWGG 8 cut(s) 201, 928, 1123, 1510, 2019, 3986, 5431, 5822
PspFI CCCAGC 3 cut(s) 462, 3779, 5052
PspGI CCWGG 8 cut(s) 201, 928, 1123, 1510, 2019, 3986, 5431, 5822
PspOMI GGGCCC 2 cut(s) 1606, 4935
PsrI GAACNNNNNNTAC 2 cut(s) 693, 725
PstI CTGCAG 5 cut(s) 2719, 4446, 4459, 5146, 5373
PstNI CAGNNNCTG 1 cut(s) 2264
PsuI RGATCY 5 cut(s) 366, 3003, 4035, 5428, 5503
PsyI GACNNNGTC 1 cut(s) 1060
PvuII CAGCTG 1 cut(s) 2876
RsaI GTAC 9 cut(s) 614, 1027, 1387, 1545, 1656, 1827, 2808, 3723, 5463
RsaNI GTAC 9 cut(s) 613, 1026, 1386, 1544, 1655, 1826, 2807, 3722, 5462
RseI CAYNNNNRTG 7 cut(s) 620, 1002, 2079, 3196, 4424, 5447, 5733
SacI GAGCTC 1 cut(s) 3633
SapI GCTCTTC 1 cut(s) 3638
ScaI AGTACT 1 cut(s) 1027
SchI GAGTC 8 cut(s) 500, 1477, 2414, 2613, 3027, 4493, 5383, 5868
ScrFI CCNGG 8 cut(s) 203, 930, 1125, 1512, 2021, 3988, 5433, 5824
SfcI CTRYAG 8 cut(s) 2005, 2265, 2715, 4442, 4455, 4925, 5142, 5369
SinI GGWCC 6 cut(s) 71, 188, 2501, 2538, 2866, 4789
SmiMI CAYNNNNRTG 7 cut(s) 620, 1002, 2079, 3196, 4424, 5447, 5733
SmlI CTYRAG 6 cut(s) 797, 872, 1568, 3418, 3664, 3911
SmoI CTYRAG 6 cut(s) 797, 872, 1568, 3418, 3664, 3911
SnaBI TACGTA 2 cut(s) 444, 5531
SpeI ACTAGT 1 cut(s) 1499
SphI GCATGC 3 cut(s) 2909, 4627, 5489
SseBI AGGCCT 1 cut(s) 3662
SspI AATATT 5 cut(s) 1747, 2963, 3090, 3217, 5060
SstI GAGCTC 1 cut(s) 3633
StuI AGGCCT 1 cut(s) 3662
StyD4I CCNGG 8 cut(s) 201, 928, 1123, 1510, 2019, 3986, 5431, 5822
StyI CCWWGG 3 cut(s) 191, 718, 4939
TaiI ACGT 9 cut(s) 343, 446, 1451, 1689, 3541, 4472, 5463, 5533, 5584
TaqI TCGA 8 cut(s) 281, 1967, 2638, 3564, 3706, 4173, 4214, 5157
TaqII GACCGA 1 cut(s) 1651
TatI WGTACW 5 cut(s) 612, 1025, 1543, 1654, 2806
TauI GCSGC 1 cut(s) 3585
TseFI GTSAC 6 cut(s) 336, 1524, 2416, 2978, 3544, 4465
Tsp45I GTSAC 6 cut(s) 336, 1524, 2416, 2978, 3544, 4465
TspGWI ACGGA 2 cut(s) 541, 2530
Tth111I GACNNNGTC 1 cut(s) 1060
Van91I CCANNNNNTGG 2 cut(s) 1174, 2616
Vha464I CTTAAG 1 cut(s) 3664
VneI GTGCAC 1 cut(s) 5188
VpaK11BI GGWCC 6 cut(s) 71, 188, 2501, 2538, 2866, 4789
VspI ATTAAT 3 cut(s) 1994, 2067, 2462
XbaI TCTAGA 1 cut(s) 2573
XcmI CCANNNNNNNNNTGG 1 cut(s) 3192
XmaJI CCTAGG 1 cut(s) 4939
XmiI GTMKAC 3 cut(s) 2645, 4812, 5533
XmnI GAANNNNTTC 2 cut(s) 3260, 5236
ZrmI AGTACT 1 cut(s) 1027
Zsp2I ATGCAT 6 cut(s) 1300, 1674, 2395, 2907, 3226, 4952
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.