FvH4_2g00141

Reverse transcriptase (RNA-dependent DNA polymerase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000278
Physical Location & Seq
Forward (+)
246116 .. 249823
3708 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g00141.t1

Sequence Viewer

Length: 2409 bp
ATGGTAGACAGCAAAGGCATGGTCGAAGCAGAAGATGTGAAATCGTTCATTAAACCCTTAAATGGCAAGGATGACTTCACACTTTGGCGGAGGAGGATGAAGAGTGTCCTGATTCTACAAGATCTGTACGAGGCTGTTCTCGGGATAGAGAATAAGGCAGTTGATATGGCAGATAAGGTTTGGCAGAAGATGGACAAGAAGGCAAGGAGCACAATAGAGTTGCATCTTGCAGATCATGTACTGGGTCGTGTATTGGATAGTGGAGGTGACAATATGAGCAGCAAAGACACCTGGGATTATCTTGAGAAGGTTTATGCGGGTAAGGGTCCTATTGGTGAGAATATGGACAGCAGCAAGACATTGGACAATGTCAAGGAGGCTGACGCAGATAAATTCTTCTTGAAAGAAAAACTCTATAGTCTTCGGATGGAAGAAGGCAGGGATTTGCAGGAACATCTGAATAAATTTCAGATTTGTGTTGCCAATTTATCAGAGATGAGTAGCGAGACCGAGAGCTCTCAAGGAGGCCTTCAAGTCAAGGGCAAGGAGACAAGTCAGTTGACAAGTAAAGAGGATAAATCTGGTAACAAGGGTACAACAAAATCCAAGAAGAAGTACAAGGAAGGTTGCTTCAAATGTGGTGCTACAGACCACTTGAAAAGAAACTGCAAAGAAGGGAGGATGGAAACACAGGTGACGGCAGGGAGTTCAAGCATAGCCAACATAGTCATCAGAAAAGATGAAGCTGATTGTGAACTTCTTGTGGAACTCTACAAGGAAAGTAAGGCAGAAGGTGTTGGTGTTTGCAAACTGAACTTCTACAAGGATGATGCTCTTGGGAAGCAGAAGGTGGCATTCAAGTGCATCACGGTGAAGGATAATCTCCAGGAAAACGGGGCTGCTAGAAGGATGAAGAAAACTTTTTTTGAGAAAGAAAGAGGCATGCCGCTGCATGCTGGGTTACCAGACACGTTTTGGAAAGAAGCAGTTAACCATGCGTGTTATTTGGTTAATCGGTCACCATCAAAGGTCTTGGACTTCAAGTGTGCAGAGGAGGTATGGTCAAGAGAACCAGCTGGGTATTCTAACTTAAGGATGTTTGGTAGCAGTGCGTATGCTCATATTTCGAGCAATAAGCAAACCAAACTCAAGCCAAATTTTCTGGCTATTAAGAAAGGAGTAAAGGGCTACAAGTTATGGAATATTATCAGCAAGAAAAAGGTTCTAAGTAGATATGTTATTGATGATAAGACTATGCCGTTTAATGAAGTAAAGACAAGTGAGGTGAAGATGAAGACTGATGTTGGTGTTGTAGATAAAGCAACAGAAGTTCCTCTAACCAAAGAGATCATGGAAGAGACTCCAGCTCAGGTGGAGCAAATTGAGCAGGTGAAGCATGATAAAGGGGTTATTGAGGTAGAAGTCTTAGAGCGGCAACAACAGTCACTAATACAAGCTCAGGTGGAGCAATTAGCACTGCGAACCTACGACTCAGTTCGTCAGTCGCTTGAGCAGGAGTTCCAGAGAAGCATAGCATTGGACAAGTATGCTCTAAGTTTCAGTCCAGGAGATCCAACCACGTATCAAGAAGCTATAGCAAAATATATACGGGAAAGTTGGATGGGAGCTATGTTAGAACAGAAGAAGTCCATACCAAAGGACTTGGTTCAGGAGTCGGTTCCTAAACCCAAAGAAGGAAATCTAGTTGGCTGTAAATGGGTTTATAGGAAAAGGGAAGATATGCATGATAAGGAAGTCATGAGTTGCAAAGCTCGGGTATTGGCTAAGGAAATCTTCAAAAGACCTGGTTTAACTCAAGGATTGAGCATTGTGTCAAAGTATGTGGTGAATCCGGGTAGACGATACTGGCAAGCAGCGACGTGGATTCTGTATTACTTGAGAAACACAAAGGAGCATGAATTTCTGTTTGAAAGACAACAAGAACAAACATGCATTACTGGTCATGAGAGGTCGGATTTGGCAGGAGACTTAGACAAAGGAAGATCTGCAACAAGTAATGTATTTACTTGCAACAAAGGTTCAGGAAGTTGTAGAACAACTATGGAATCAGCTGAGATATTGTCTACCACTGAAGTAGATTGCATGGCAGTAACAGAAGCTTCCGTAGAGGCGTTATGGCTTAATGGGTTAATAAGTGAGTTTGAGATTCAACAAGAAGCTGTGGTGAAGAATGGTGTCATCAATTTGACAAGGAATCAAGTGTTTCAAGCAGAGAGTGAACATGTTGATGTTTGTGGTCATTGCAGCGAAGGTTGGGTCAGCTCAGGGAAGATAACAACTGAGGAAGAAGCACATTCAAGGGAGAATGTTTCAGATTGTTTTACCAGGACCATTGCCAGGGAGAAGTTCAAGCATTGCTTGGACTTCCTCAGTGTCACAGGTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1

Protein Analysis

803

Amino Acids

90.73

Weight (kDa)

7.9

Isoelectric Point (pI)

44.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_2 PF14223 33 - 171 2.3e-14 gag-polypeptide of LTR copia-type
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000278)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G29785
fragaria_vesca FvH4_1g10046 FvH4_1g17351 FvH4_1g18441 FvH4_1g24222 FvH4_2g00141 FvH4_2g05931 FvH4_2g06671 FvH4_2g14030 FvH4_2g15291 FvH4_2g22611 FvH4_2g22612 FvH4_2g26380 FvH4_2g39202 FvH4_2g39202 FvH4_3g06691 FvH4_3g08580 FvH4_3g20502 FvH4_3g20503 FvH4_3g22421 FvH4_3g42580 FvH4_4g02061 FvH4_4g06794 FvH4_4g08661 FvH4_4g11771 FvH4_4g14601 FvH4_4g14721 FvH4_4g20921 FvH4_4g33601 FvH4_4g33602 FvH4_5g07492 FvH4_5g20321 FvH4_5g21591 FvH4_5g30343 FvH4_5g37875 FvH4_6g04372 FvH4_6g04373 FvH4_6g04373 FvH4_6g21377 FvH4_6g23760 FvH4_6g29471 FvH4_6g43142 FvH4_7g14351 FvH4_7g14352 FvH4_7g22582 FvH4_7g22583 FvH4_7g22584
malus_domestica MD00G1179400.v1.1 MD01G1170500.v1.1 MD03G1286700.v1.1 MD04G1054600.v1.1 MD04G1071700.v1.1 MD04G1193900.v1.1 MD08G1054300.v1.1 MD08G1217500.v1.1 MD09G1034600.v1.1 MD09G1098100.v1.1 MD10G1018300.v1.1 MD10G1220000.v1.1 MD10G1250300.v1.1 MD12G1014400.v1.1 MD15G1021000.v1.1 MD16G1112200.v1.1
prunus_persica Prupe.1G188900_v2.0.a1 Prupe.1G258000_v2.0.a1 Prupe.4G233400_v2.0.a1
pyrus_communis pycom01g10100 pycom02g01340 pycom03g19700 pycom05g01320 pycom07g16000 pycom07g16520 pycom11g26500 pycom13g09380 pycom13g13960 pycom14g08180 pycom14g16810 pycom16g09590
rosa_chinensis RchiOBHm_Chr1g0319891 RchiOBHm_Chr1g0333711 RchiOBHm_Chr2g0115331 RchiOBHm_Chr2g0143411 RchiOBHm_Chr2g0167631 RchiOBHm_Chr3g0448581 RchiOBHm_Chr3g0494991 RchiOBHm_Chr4g0442471 RchiOBHm_Chr6g0262311 RchiOBHm_Chr6g0301831 RchiOBHm_Chr6g0301841 RchiOBHm_Chr7g0183451
rosa_roxburghii Rroxscaffold_1G00039280 Rroxscaffold_1G00047670 Rroxscaffold_1G00066330 Rroxscaffold_2G00107510 Rroxscaffold_3G00255410 Rroxscaffold_4G00301460 Rroxscaffold_5G00352730 Rroxscaffold_7G00166350 Rroxscaffold_7G00166360 Rroxscaffold_7G00203820
rosa_rugosa Rorug03G0240500 Rorug05G0191700 Rorug06G0310200
rosa_samantha Rh2BG287000 Rh4CG073500 Rh4CG176200 Rh4CG191400 Rh5BG356100 Rh5BG434100 Rh6AG110600 Rh6AG421900 Rh6AG422000 Rh6AG422100 Rh6BG428600 Rh6BG428700 Rh6BG428800 Rh6CG435200 Rh6CG435300 Rh6CG435400 Rh6DG422000 Rh6DG422100 Rh6DG422200 Rh7BG190500 Rh7CG172800 Rh7CG225200
rosa_wichuraiana Rw2G044060 Rw6G036540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1378
Acc36I ACCTGC 1 cut(s) 1378
AccBSI CCGCTC 1 cut(s) 1432
AccI GTMKAC 3 cut(s) 6, 1858, 2084
AciI CCGC 4 cut(s) 88, 317, 947, 1432
AclWI GGATC 1 cut(s) 1565
AcsI RAATTY 4 cut(s) 392, 464, 1156, 1919
AcuI CTGAAG 1 cut(s) 2112
AfaI GTAC 4 cut(s) 128, 240, 595, 617
AfiI CCNNNNNNNGG 3 cut(s) 62, 1694, 2358
AflII CTTAAG 1 cut(s) 1090
AflIII ACRYGT 2 cut(s) 969, 2242
AjiI CACGTC 1 cut(s) 1881
AjnI CCWGG 6 cut(s) 290, 885, 1564, 1804, 2345, 2357
Alw21I GWGCWC 2 cut(s) 212, 518
Alw26I GTCTC 4 cut(s) 500, 542, 1352, 1980
AlwI GGATC 1 cut(s) 1565
Ama87I CYCGRG 2 cut(s) 140, 1773
AoxI GGCC 1 cut(s) 526
ApeKI GCWGC 6 cut(s) 279, 351, 899, 949, 1874, 2265
ApoI RAATTY 4 cut(s) 392, 464, 1156, 1919
ArsI GACNNNNNNTTYG 3 cut(s) 38, 2262, 2294
Asp700I GAANNNNTTC 1 cut(s) 44
AspS9I GGNCC 2 cut(s) 326, 2349
AsuC2I CCSGG 1 cut(s) 1854
AsuHPI GGTGA 9 cut(s) 278, 347, 706, 883, 1011, 1297, 1402, 1858, 2197
AvaI CYCGRG 2 cut(s) 140, 1773
AvaII GGWCC 2 cut(s) 326, 2349
BanII GRGCYC 1 cut(s) 518
BbsI GAAGAC 2 cut(s) 413, 1301
Bbv12I GWGCWC 2 cut(s) 212, 518
BbvI GCAGC 6 cut(s) 291, 363, 886, 936, 1886, 2277
BccI CCATC 5 cut(s) 184, 421, 676, 1030, 1615
BceAI ACGGC 2 cut(s) 714, 1243
BciT130I CCWGG 6 cut(s) 292, 887, 1566, 1806, 2347, 2359
BcnI CCSGG 1 cut(s) 1854
BcoDI GTCTC 4 cut(s) 500, 542, 1352, 1980
BfaI CTAG 2 cut(s) 903, 1703
BfmI CTRYAG 3 cut(s) 415, 645, 1593
BfrI CTTAAG 1 cut(s) 1090
BfuAI ACCTGC 1 cut(s) 1378
BglII AGATCT 2 cut(s) 121, 2003
BisI GCNGC 8 cut(s) 280, 352, 900, 947, 950, 1433, 1875, 2266
BlsI GCNGC 8 cut(s) 281, 353, 901, 948, 951, 1434, 1876, 2267
Bme1390I CCNGG 7 cut(s) 292, 887, 1566, 1806, 1854, 2347, 2359
Bme18I GGWCC 2 cut(s) 326, 2349
BmeT110I CYCGRG 2 cut(s) 140, 1773
BmgBI CACGTC 1 cut(s) 1881
BmgT120I GGNCC 2 cut(s) 326, 2349
BmiI GGNNCC 2 cut(s) 327, 1680
BmrFI CCNGG 7 cut(s) 292, 887, 1566, 1806, 1854, 2347, 2359
BmrI ACTGGG 1 cut(s) 251
BmsI GCATC 3 cut(s) 232, 820, 873
BmuI ACTGGG 1 cut(s) 251
BpiI GAAGAC 2 cut(s) 413, 1301
BpmI CTGGAG 2 cut(s) 869, 1347
Bpu10I CCTNAGC 4 cut(s) 1368, 1458, 1785, 2284
BpuEI CTTGAG 6 cut(s) 323, 504, 1133, 1529, 1800, 1918
BpuMI CCSGG 1 cut(s) 1854
BsaAI YACGTR 1 cut(s) 1581
BsaI GGTCTC 1 cut(s) 500
BsaJI CCNNGG 2 cut(s) 291, 2358
BsaXI ACNNNNNCTCC 2 cut(s) 1046, 1076
Bsc4I CCNNNNNNNGG 3 cut(s) 62, 1694, 2358
Bse1I ACTGG 3 cut(s) 246, 1871, 1963
Bse3DI GCAATG 3 cut(s) 2260, 2352, 2374
BseBI CCWGG 6 cut(s) 292, 887, 1566, 1806, 2347, 2359
BseDI CCNNGG 2 cut(s) 291, 2358
BseGI GGATG 8 cut(s) 76, 102, 432, 687, 832, 915, 1101, 1626
BseLI CCNNNNNNNGG 3 cut(s) 62, 1694, 2358
BseMI GCAATG 3 cut(s) 2260, 2352, 2374
BseMII CTCAG 7 cut(s) 1382, 1472, 1506, 2064, 2292, 2298, 2404
BseNI ACTGG 3 cut(s) 246, 1871, 1963
BseRI GAGGAG 2 cut(s) 106, 1067
BseXI GCAGC 6 cut(s) 291, 363, 886, 936, 1886, 2277
BseYI CCCAGC 2 cut(s) 956, 1076
BsgI GTGCAG 1 cut(s) 1068
BshFI GGCC 1 cut(s) 528
BsiHKAI GWGCWC 2 cut(s) 212, 518
BsiHKCI CYCGRG 2 cut(s) 140, 1773
BsiSI CCGG 1 cut(s) 1853
BslI CCNNNNNNNGG 3 cut(s) 62, 1694, 2358
BsmAI GTCTC 4 cut(s) 500, 542, 1352, 1980
BsmI GAATGC 1 cut(s) 854
BsnI GGCC 1 cut(s) 528
Bso31I GGTCTC 1 cut(s) 500
BsoBI CYCGRG 2 cut(s) 140, 1773
Bsp1286I GDGCHC 2 cut(s) 212, 518
Bsp143I GATC 5 cut(s) 121, 232, 1347, 1570, 2003
BspACI CCGC 4 cut(s) 88, 317, 947, 1432
BspANI GGCC 1 cut(s) 528
BspCNI CTCAG 7 cut(s) 1381, 1471, 1505, 2065, 2293, 2297, 2403
BspHI TCATGA 2 cut(s) 1758, 1963
BspLI GGNNCC 2 cut(s) 327, 1680
BspMI ACCTGC 1 cut(s) 1378
BspPI GGATC 1 cut(s) 1565
BspTI CTTAAG 1 cut(s) 1090
BspTNI GGTCTC 1 cut(s) 500
BsrBI CCGCTC 1 cut(s) 1432
BsrDI GCAATG 3 cut(s) 2260, 2352, 2374
BsrI ACTGG 3 cut(s) 246, 1871, 1963
BssECI CCNNGG 2 cut(s) 291, 2358
BssMI GATC 5 cut(s) 121, 232, 1347, 1570, 2003
Bst2UI CCWGG 6 cut(s) 292, 887, 1566, 1806, 2347, 2359
Bst4CI ACNGT 2 cut(s) 871, 1443
Bst6I CTCTTC 2 cut(s) 95, 1350
BstAFI CTTAAG 1 cut(s) 1090
BstBAI YACGTR 1 cut(s) 1581
BstC8I GCNNGC 3 cut(s) 944, 954, 1872
BstEII GGTNACC 2 cut(s) 960, 1017
BstF5I GGATG 8 cut(s) 76, 102, 432, 687, 832, 915, 1101, 1626
BstKTI GATC 5 cut(s) 124, 235, 1350, 1573, 2006
BstMAI GTCTC 4 cut(s) 500, 542, 1352, 1980
BstMBI GATC 5 cut(s) 121, 232, 1347, 1570, 2003
BstMWI GCNNNNNNNGC 2 cut(s) 1384, 1393
BstNI CCWGG 6 cut(s) 292, 887, 1566, 1806, 2347, 2359
BstNSI RCATGY 4 cut(s) 946, 956, 1953, 2246
BstPI GGTNACC 2 cut(s) 960, 1017
BstSCI CCNGG 7 cut(s) 290, 885, 1564, 1804, 1852, 2345, 2357
BstSFI CTRYAG 3 cut(s) 415, 645, 1593
BstV1I GCAGC 6 cut(s) 291, 363, 886, 936, 1886, 2277
BstV2I GAAGAC 2 cut(s) 413, 1301
BstX2I RGATCY 3 cut(s) 121, 1570, 2003
BstYI RGATCY 3 cut(s) 121, 1570, 2003
BsuRI GGCC 1 cut(s) 528
BtrI CACGTC 1 cut(s) 1881
BtsCI GGATG 8 cut(s) 76, 102, 432, 687, 832, 915, 1101, 1626
BtsI GCAGTG 2 cut(s) 1114, 1475
BtsIMutI CAGTG 4 cut(s) 1114, 1475, 2088, 2398
BveI ACCTGC 1 cut(s) 1378
Cac8I GCNNGC 3 cut(s) 944, 954, 1872
CciI TCATGA 2 cut(s) 1758, 1963
Cfr13I GGNCC 2 cut(s) 326, 2349
CseI GACGC 1 cut(s) 392
CsiI ACCWGGT 1 cut(s) 1804
Csp6I GTAC 4 cut(s) 127, 239, 594, 616
CspCI CAANNNNNGTGG 2 cut(s) 1824, 1859
CviQI GTAC 4 cut(s) 127, 239, 594, 616
DpnI GATC 5 cut(s) 123, 234, 1349, 1572, 2005
DpnII GATC 5 cut(s) 121, 232, 1347, 1570, 2003
Eam1104I CTCTTC 2 cut(s) 95, 1350
EarI CTCTTC 2 cut(s) 95, 1350
EciI GGCGGA 1 cut(s) 103
Ecl136II GAGCTC 1 cut(s) 516
Eco147I AGGCCT 1 cut(s) 528
Eco24I GRGCYC 1 cut(s) 518
Eco31I GGTCTC 1 cut(s) 500
Eco47I GGWCC 2 cut(s) 326, 2349
Eco53kI GAGCTC 1 cut(s) 516
Eco57I CTGAAG 1 cut(s) 2112
Eco88I CYCGRG 2 cut(s) 140, 1773
Eco91I GGTNACC 2 cut(s) 960, 1017
EcoICRI GAGCTC 1 cut(s) 516
EcoO109I RGGNCCY 1 cut(s) 326
EcoO65I GGTNACC 2 cut(s) 960, 1017
EcoRII CCWGG 6 cut(s) 290, 885, 1564, 1804, 2345, 2357
EcoT22I ATGCAT 2 cut(s) 1746, 1955
EcoT38I GRGCYC 1 cut(s) 518
FalI AAGNNNNNCTT 8 cut(s) 59, 91, 513, 545, 1778, 1810, 2363, 2395
FauI CCCGC 1 cut(s) 310
FblI GTMKAC 3 cut(s) 6, 1858, 2084
Fnu4HI GCNGC 8 cut(s) 280, 352, 900, 947, 950, 1433, 1875, 2266
FokI GGATG 8 cut(s) 83, 109, 439, 694, 839, 922, 1108, 1633
FriOI GRGCYC 1 cut(s) 518
Fsp4HI GCNGC 8 cut(s) 280, 352, 900, 947, 950, 1433, 1875, 2266
FspBI CTAG 2 cut(s) 903, 1703
GluI GCNGC 8 cut(s) 280, 352, 900, 947, 950, 1433, 1875, 2266
GsaI CCCAGC 2 cut(s) 960, 1080
GsuI CTGGAG 2 cut(s) 869, 1347
HaeIII GGCC 1 cut(s) 528
HapII CCGG 1 cut(s) 1853
HgaI GACGC 1 cut(s) 392
HincII GTYRAC 2 cut(s) 561, 991
HindII GTYRAC 2 cut(s) 561, 991
HindIII AAGCTT 1 cut(s) 2118
HinfI GANTC 9 cut(s) 112, 1360, 1490, 1673, 1849, 1885, 2066, 2167, 2215
HpaI GTTAAC 1 cut(s) 991
HpaII CCGG 1 cut(s) 1853
HphI GGTGA 9 cut(s) 278, 347, 706, 883, 1011, 1297, 1402, 1858, 2197
Hpy166II GTNNAC 7 cut(s) 7, 561, 755, 991, 1859, 2085, 2240
Hpy188I TCNGA 7 cut(s) 426, 459, 471, 493, 734, 1975, 2335
Hpy8I GTNNAC 7 cut(s) 7, 561, 755, 991, 1859, 2085, 2240
Hpy99I CGWCG 1 cut(s) 1882
HpyCH4III ACNGT 2 cut(s) 871, 1443
HpyCH4IV ACGT 3 cut(s) 971, 1580, 1880
HpyF10VI GCNNNNNNNGC 2 cut(s) 1384, 1393
HpySE526I ACGT 3 cut(s) 971, 1580, 1880
KspAI GTTAAC 1 cut(s) 991
Kzo9I GATC 5 cut(s) 121, 232, 1347, 1570, 2003
LmnI GCTCC 5 cut(s) 207, 1375, 1465, 1625, 1912
Lsp1109I GCAGC 6 cut(s) 291, 363, 886, 936, 1886, 2277
LweI GCATC 3 cut(s) 232, 820, 873
MabI ACCWGGT 1 cut(s) 1804
MaeI CTAG 2 cut(s) 903, 1703
MaeII ACGT 3 cut(s) 971, 1580, 1880
MaeIII GTNAC 8 cut(s) 266, 584, 694, 960, 1017, 1443, 2110, 2395
MalI GATC 5 cut(s) 123, 234, 1349, 1572, 2005
MbiI CCGCTC 1 cut(s) 1432
MboI GATC 5 cut(s) 121, 232, 1347, 1570, 2003
MflI RGATCY 3 cut(s) 121, 1570, 2003
MhlI GDGCHC 2 cut(s) 212, 518
MluCI AATT 8 cut(s) 392, 464, 484, 1156, 1380, 1469, 1919, 2203
MlyI GAGTC 3 cut(s) 1354, 1484, 1682
MmeI TCCRAC 3 cut(s) 1598, 1598, 1953
Mph1103I ATGCAT 2 cut(s) 1746, 1955
MroXI GAANNNNTTC 1 cut(s) 44
MslI CAYNNNNRTG 3 cut(s) 859, 869, 2247
MspA1I CMGCKG 3 cut(s) 949, 1076, 2072
MspCI CTTAAG 1 cut(s) 1090
MspI CCGG 1 cut(s) 1853
MspR9I CCNGG 7 cut(s) 292, 887, 1566, 1806, 1854, 2347, 2359
Mva1269I GAATGC 1 cut(s) 854
MvaI CCWGG 6 cut(s) 292, 887, 1566, 1806, 2347, 2359
MwoI GCNNNNNNNGC 2 cut(s) 1384, 1393
NciI CCSGG 1 cut(s) 1854
NdeII GATC 5 cut(s) 121, 232, 1347, 1570, 2003
NlaIV GGNNCC 2 cut(s) 327, 1680
NmuCI GTSAC 5 cut(s) 266, 694, 1017, 1443, 2395
NsiI ATGCAT 2 cut(s) 1746, 1955
NspI RCATGY 4 cut(s) 946, 956, 1953, 2246
PaeI GCATGC 2 cut(s) 946, 956
PagI TCATGA 2 cut(s) 1758, 1963
PaqCI CACCTGC 1 cut(s) 1378
PceI AGGCCT 1 cut(s) 528
PciI ACATGT 1 cut(s) 2242
PctI GAATGC 1 cut(s) 854
PdmI GAANNNNTTC 1 cut(s) 44
PfeI GAWTC 6 cut(s) 112, 1849, 1885, 2066, 2167, 2215
PflFI GACNNNGTC 1 cut(s) 368
PfoI TCCNGGA 2 cut(s) 885, 1564
PkrI GCNGC 8 cut(s) 281, 353, 901, 948, 951, 1434, 1876, 2267
PleI GAGTC 3 cut(s) 1354, 1484, 1681
PpsI GAGTC 3 cut(s) 1354, 1484, 1681
Ppu21I YACGTR 1 cut(s) 1581
PpuMI RGGWCCY 1 cut(s) 326
PscI ACATGT 1 cut(s) 2242
Psp124BI GAGCTC 1 cut(s) 518
Psp5II RGGWCCY 1 cut(s) 326
Psp6I CCWGG 6 cut(s) 290, 885, 1564, 1804, 2345, 2357
PspEI GGTNACC 2 cut(s) 960, 1017
PspFI CCCAGC 2 cut(s) 956, 1076
PspGI CCWGG 6 cut(s) 290, 885, 1564, 1804, 2345, 2357
PspN4I GGNNCC 2 cut(s) 327, 1680
PspPI GGNCC 2 cut(s) 326, 2349
PspPPI RGGWCCY 1 cut(s) 326
PsuI RGATCY 3 cut(s) 121, 1570, 2003
PsyI GACNNNGTC 1 cut(s) 368
PvuII CAGCTG 2 cut(s) 1076, 2072
RsaI GTAC 4 cut(s) 128, 240, 595, 617
RsaNI GTAC 4 cut(s) 127, 239, 594, 616
RseI CAYNNNNRTG 3 cut(s) 859, 869, 2247
SacI GAGCTC 1 cut(s) 518
SatI GCNGC 8 cut(s) 280, 352, 900, 947, 950, 1433, 1875, 2266
Sau3AI GATC 5 cut(s) 121, 232, 1347, 1570, 2003
Sau96I GGNCC 2 cut(s) 326, 2349
SchI GAGTC 3 cut(s) 1354, 1484, 1682
ScrFI CCNGG 7 cut(s) 292, 887, 1566, 1806, 1854, 2347, 2359
SduI GDGCHC 2 cut(s) 212, 518
SexAI ACCWGGT 1 cut(s) 1804
SfaNI GCATC 3 cut(s) 232, 820, 873
SfcI CTRYAG 3 cut(s) 415, 645, 1593
SinI GGWCC 2 cut(s) 326, 2349
SmiMI CAYNNNNRTG 3 cut(s) 859, 869, 2247
SmlI CTYRAG 7 cut(s) 302, 519, 1090, 1148, 1508, 1815, 1897
SmoI CTYRAG 7 cut(s) 302, 519, 1090, 1148, 1508, 1815, 1897
SphI GCATGC 2 cut(s) 946, 956
Sse9I AATT 8 cut(s) 392, 464, 484, 1156, 1380, 1469, 1919, 2203
SseBI AGGCCT 1 cut(s) 528
SsiI CCGC 4 cut(s) 88, 317, 947, 1432
SspI AATATT 1 cut(s) 1204
SspMI CTAG 2 cut(s) 903, 1703
SstI GAGCTC 1 cut(s) 518
StuI AGGCCT 1 cut(s) 528
StyD4I CCNGG 7 cut(s) 290, 885, 1564, 1804, 1852, 2345, 2357
TaaI ACNGT 2 cut(s) 871, 1443
TaiI ACGT 3 cut(s) 974, 1583, 1883
TaqI TCGA 2 cut(s) 24, 1127
TaqII GACCGA 2 cut(s) 524, 1005
TasI AATT 8 cut(s) 392, 464, 484, 1156, 1380, 1469, 1919, 2203
TatI WGTACW 2 cut(s) 238, 615
TauI GCSGC 2 cut(s) 949, 1435
TfiI GAWTC 6 cut(s) 112, 1849, 1885, 2066, 2167, 2215
TscAI CASTG 4 cut(s) 1114, 1482, 2095, 2398
TseFI GTSAC 5 cut(s) 266, 694, 1017, 1443, 2395
TseI GCWGC 6 cut(s) 279, 351, 899, 949, 1874, 2265
Tsp45I GTSAC 5 cut(s) 266, 694, 1017, 1443, 2395
TspDTI ATGAA 7 cut(s) 37, 113, 756, 926, 1281, 1307, 1932
TspGWI ACGGA 1 cut(s) 2113
TspRI CASTG 4 cut(s) 1114, 1482, 2095, 2398
Tth111I GACNNNGTC 1 cut(s) 368
Vha464I CTTAAG 1 cut(s) 1090
VpaK11BI GGWCC 2 cut(s) 326, 2349
XapI RAATTY 4 cut(s) 392, 464, 1156, 1919
XceI RCATGY 4 cut(s) 946, 956, 1953, 2246
XcmI CCANNNNNNNNNTGG 2 cut(s) 972, 1348
XmiI GTMKAC 3 cut(s) 6, 1858, 2084
XmnI GAANNNNTTC 1 cut(s) 44
XspI CTAG 2 cut(s) 903, 1703
Zsp2I ATGCAT 2 cut(s) 1746, 1955
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.