FvH4_6g21377

Mitochondrial protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000278
Physical Location & Seq
Reverse (-)
14971481 .. 14974084
2604 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g21377.t1

Sequence Viewer

Length: 2604 bp
ATGGAAGGATCACGGAGTACCATGATCAGACTCAACCACTCCAACTGGATTACGTGGAAGCCGAGAATAGAGGATATTCTCTATTGCAAAGATTTGCACGAGCCGATCGAGGGAGACGATGCCAAGCCAGAAGGGACTACACCAGCAATTTGGACGAAGATGAACCGCAAAGCCATCGGTCATATTCGTGAATGGGTGGACGACAGTGTGTTCCACCATGTTGCCAACGAAACCAGTGCGCATGAGCTATGGTTGAAGTTGGAGTCCTTGTTCGAGAAAAAGAATACTGCCAAGAAAGCTTTTCTCATTAAAGAGCTCGTCAACATGAAGTATCGGGGAGGCGTTCGAGTGACTGAGCACCTCAACAACTTCCAGAGTGTACTCAATCAATTAGCCACGATGAGCATGAAGATTGATGATGAGTTACAAGCCTTGTTGCTGCTCAGGTCGTTACCAGATAGTTGGGAGACATTTGTTGTTACTGTATTTGTTGTTACTGTAAGTAACTCTGCTCCTAATGGTGTATTATCTATGGAAAATATTAAAGATAATATGATGAACGAAGAAACCAGAAGGAGTTCTTCGTCAAGTTCAGAAAATGGTCAATTCTTCGTTGCGGAGAATAGAGGAAGGAGCAAGAGCAGAGAACCGAGAGGCCATGGAAGAAGTATGCGCAAATCCAGAACAAGATTTAGCGGAAAGTGTCATCATTGTGGTCTTCCAGGCCACATGAAAAAAAATTGTTACAAGTTGAAGCGGGAATTCAAAAAAGGGCGAGAAGACGCTACTCATCAACAAAAGGAGGACACGAACAATGTTGCTGCTGCAGTGTCTCATGATGAGTGTGAGTTCCTGTCACTCGGAGGTGAATGTCTATTGGTAGATAATCCTGGAGTTCAATGGATCATTGATTCCGGAGCCTCGTTTCATGCCACGTCCCACGTAGAGTTCTTCACTACGTACAAAGTGGGCAATGACAGCTACTCCAATATTGCTAGGATTGGAGATATTTGCTTGCAGACGGATGTGGGCTACCAGTTAATGCTGAAAGATGTGAGACATGTTCCTGGTCTACGTCTCAATCTTATGTCAACCGGTGTACTTGATCAACAAGGCTTTCATCATCATGGCGGTGATGGAAAGTGGAAGCTTACCAAAGGATCTCTTGTAGTTGCTAGAGGAAAAATGTGTTGCACGCTCTACAAGACTTATGGGAAGATTTGCAAGAGTGAGCTAAATGCAGTCGAGGAATCCTCTCCAAGTTTGTGGCATAGACGTCTTGGCCACATGAGTGAGAAAGGATTGCAGATGTTGGCGAAGAAGTCGCTAATTCCTGTTGCCAAAGGTAACTCACTAGACTCTTGCGATTATTGTTTATTTGGTAAGCAGAAGAAAATCAGTTTTTCGAAGTCTTTCACGAGGAAAGAAAATATTCTAGATCTTGTACATTCAGATGTGTGTGGTCCGATGGAAGTCGAATCATTGAGGATGCATAAGTATTTTGTAACATATATCGATGATGCTTCACGGAAAGTTTGGGTTTATTTGTTGAAATCCAAAGACCAGGTGTTCCAGACGTTTACAATTTTCCACACCATGGTGGAAAGGGAGACTGGAAGAAAATTGAGGTGCCTTCGCAGTGACAATGGCGGAGAGTACACATCGCATGAATTCAGAGATTATTGTGCGAAGTATGGCATCAGACATGAGAAGACGGTTCCTAGAACACCCCAACATAATGGCGTAGCGGAGCGGATGAACCGTACCATTATGGAGAAAGTCAAATGCATGTTAAAGATGGCCAAATTGTCAAAAGAATTTTGGGGAGAAGCTGTGAAGACTGCGTGTTACCTCATCAACAGAGCACCGTGTGTACCTTTGGAATTTGAGACTCCAAATGGAGTTTGGACCGGTAAAGATGCATCATACTCGCATCTGAGAGTATTTGGATGTAAAGCATTCGCACATGTATCCAAGGAGCAGAGATCAAAGCTGGATGACAAGGCGTTGCCATGCATATTTGTTGGCTATGGCGATGAAGAGTTTGGCTACCGGTTGTGGGATCCAAAGATGAAGAAATTCATAAGAAGCAGAGATGTGGTTTTTCATGAAAACCAATCAATTGCTGATTTCGACAAAGAGGTACTACCGAGAAATGCAGCAACTGACAGCCGCATTTATGATTCACCGTTGCAGCAAGAATCTAATGTTGGGCTACAAGGTGATAGAGTTGAAGGTGTACCAGACATGGTTGATGTGGAAGCCGAAGATGAGGGGGAGCTTGATCAGGGGGAGCAGCCTGTGAATAATCAAGCAGCAGTTCGCAGAAGCACTAGGAGGCCGATTCAATCCACCAAGTATCCATCATCACAATATATTTTGGTGACTAACGATGGAGATGATCCTTATGCAAAGTACATCCTTTTGACTAGTGACGGAGAGCCTGAAAGTTATGAGGAAGCAAAGATTCATCAAGACCACGACAAATGGAGGCTAGCCATGGAGTCTGAGATGGAATCTTTACTGAAGAACGACACCTACGAGTTGGTGAAGCTTCCTAAAGGCCGGAAAGCACTCAAGAACAAGTGTGGGTGTTCAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve zf-CCHC

Protein Analysis

868

Amino Acids

98.91

Weight (kDa)

8.55

Isoelectric Point (pI)

42.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_2 PF14223 26 - 196 3.6e-36 gag-polypeptide of LTR copia-type
Pol_BBD PF22936 301 - 373 5.9e-17 Pol polyprotein, beta-barrel domain
gag_pre-integrs PF13976 400 - 463 4.8e-16 GAG-pre-integrase domain
rve PF00665 482 - 577 2.6e-13 Integrase core domain
SH3_retrovirus PF25597 651 - 708 7.2e-25 Retroviral polymerase SH3-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000278)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G29785
fragaria_vesca FvH4_1g10046 FvH4_1g17351 FvH4_1g18441 FvH4_1g24222 FvH4_2g00141 FvH4_2g05931 FvH4_2g06671 FvH4_2g14030 FvH4_2g15291 FvH4_2g22611 FvH4_2g22612 FvH4_2g26380 FvH4_2g39202 FvH4_2g39202 FvH4_3g06691 FvH4_3g08580 FvH4_3g20502 FvH4_3g20503 FvH4_3g22421 FvH4_3g42580 FvH4_4g02061 FvH4_4g06794 FvH4_4g08661 FvH4_4g11771 FvH4_4g14601 FvH4_4g14721 FvH4_4g20921 FvH4_4g33601 FvH4_4g33602 FvH4_5g07492 FvH4_5g20321 FvH4_5g21591 FvH4_5g30343 FvH4_5g37875 FvH4_6g04372 FvH4_6g04373 FvH4_6g04373 FvH4_6g21377 FvH4_6g23760 FvH4_6g29471 FvH4_6g43142 FvH4_7g14351 FvH4_7g14352 FvH4_7g22582 FvH4_7g22583 FvH4_7g22584
malus_domestica MD00G1179400.v1.1 MD01G1170500.v1.1 MD03G1286700.v1.1 MD04G1054600.v1.1 MD04G1071700.v1.1 MD04G1193900.v1.1 MD08G1054300.v1.1 MD08G1217500.v1.1 MD09G1034600.v1.1 MD09G1098100.v1.1 MD10G1018300.v1.1 MD10G1220000.v1.1 MD10G1250300.v1.1 MD12G1014400.v1.1 MD15G1021000.v1.1 MD16G1112200.v1.1
prunus_persica Prupe.1G188900_v2.0.a1 Prupe.1G258000_v2.0.a1 Prupe.4G233400_v2.0.a1
pyrus_communis pycom01g10100 pycom02g01340 pycom03g19700 pycom05g01320 pycom07g16000 pycom07g16520 pycom11g26500 pycom13g09380 pycom13g13960 pycom14g08180 pycom14g16810 pycom16g09590
rosa_chinensis RchiOBHm_Chr1g0319891 RchiOBHm_Chr1g0333711 RchiOBHm_Chr2g0115331 RchiOBHm_Chr2g0143411 RchiOBHm_Chr2g0167631 RchiOBHm_Chr3g0448581 RchiOBHm_Chr3g0494991 RchiOBHm_Chr4g0442471 RchiOBHm_Chr6g0262311 RchiOBHm_Chr6g0301831 RchiOBHm_Chr6g0301841 RchiOBHm_Chr7g0183451
rosa_roxburghii Rroxscaffold_1G00039280 Rroxscaffold_1G00047670 Rroxscaffold_1G00066330 Rroxscaffold_2G00107510 Rroxscaffold_3G00255410 Rroxscaffold_4G00301460 Rroxscaffold_5G00352730 Rroxscaffold_7G00166350 Rroxscaffold_7G00166360 Rroxscaffold_7G00203820
rosa_rugosa Rorug03G0240500 Rorug05G0191700 Rorug06G0310200
rosa_samantha Rh2BG287000 Rh4CG073500 Rh4CG176200 Rh4CG191400 Rh5BG356100 Rh5BG434100 Rh6AG110600 Rh6AG421900 Rh6AG422000 Rh6AG422100 Rh6BG428600 Rh6BG428700 Rh6BG428800 Rh6CG435200 Rh6CG435300 Rh6CG435400 Rh6DG422000 Rh6DG422100 Rh6DG422200 Rh7BG190500 Rh7CG172800 Rh7CG225200
rosa_wichuraiana Rw2G044060 Rw6G036540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1279
Acc16I TGCGCA 2 cut(s) 240, 674
AccB1I GGYRCC 1 cut(s) 1629
AccB7I CCANNNNNTGG 1 cut(s) 1597
AccBSI CCGCTC 1 cut(s) 1753
AccI GTMKAC 1 cut(s) 1072
AccIII TCCGGA 1 cut(s) 914
AciI CCGC 9 cut(s) 166, 617, 696, 757, 1131, 1650, 1748, 1753, 2173
AclWI GGATC 6 cut(s) 16, 911, 1168, 2057, 2070, 2396
AcoI YGGCCR 2 cut(s) 1282, 1800
AcsI RAATTY 5 cut(s) 761, 1670, 1817, 1883, 2078
AcuI CTGAAG 1 cut(s) 2546
AcyI GRCGYC 1 cut(s) 1276
AdeI CACNNNGTG 1 cut(s) 1871
AfiI CCNNNNNNNGG 3 cut(s) 110, 1597, 2059
AflIII ACRYGT 2 cut(s) 1060, 1966
AgeI ACCGGT 3 cut(s) 1094, 1910, 2052
AgsI TTSAA 8 cut(s) 256, 754, 766, 899, 1552, 2234, 2348, 2598
AhlI ACTAGT 1 cut(s) 2429
AjiI CACGTC 1 cut(s) 936
AjnI CCWGG 4 cut(s) 721, 889, 1066, 1563
AjuI GAANNNNNNNTTGG 2 cut(s) 2193, 2225
AleI CACNNNNGTG 2 cut(s) 1290, 1598
AloI GAACNNNNNNTCC 2 cut(s) 254, 286
Alw21I GWGCWC 3 cut(s) 318, 360, 1867
Alw26I GTCTC 7 cut(s) 108, 461, 837, 1051, 1082, 1604, 1883
AlwI GGATC 6 cut(s) 16, 911, 1168, 2057, 2070, 2396
AlwNI CAGNNNCTG 1 cut(s) 2165
Aor13HI TCCGGA 1 cut(s) 914
AoxI GGCC 6 cut(s) 655, 724, 1282, 1800, 2339, 2563
ApeKI GCWGC 7 cut(s) 439, 821, 824, 2159, 2194, 2296, 2315
ApoI RAATTY 5 cut(s) 761, 1670, 1817, 1883, 2078
AsiGI ACCGGT 3 cut(s) 1094, 1910, 2052
Asp700I GAANNNNTTC 4 cut(s) 577, 1412, 1431, 2078
AspLEI GCGC 2 cut(s) 241, 675
AspS9I GGNCC 2 cut(s) 1463, 1908
AsuHPI GGTGA 6 cut(s) 878, 1145, 2178, 2234, 2395, 2560
AsuII TTCGAA 1 cut(s) 1406
AsuNHI GCTAGC 1 cut(s) 2494
AvaII GGWCC 2 cut(s) 1463, 1908
BalI TGGCCA 2 cut(s) 1284, 1802
BamHI GGATCC 1 cut(s) 2062
BanI GGYRCC 1 cut(s) 1629
BanII GRGCYC 1 cut(s) 318
BauI CACGAG 2 cut(s) 98, 1417
BbsI GAAGAC 4 cut(s) 710, 786, 1718, 1844
Bbv12I GWGCWC 3 cut(s) 318, 360, 1867
BbvI GCAGC 7 cut(s) 426, 808, 811, 2171, 2206, 2308, 2327
BccI CCATC 7 cut(s) 182, 1130, 1462, 1792, 2371, 2387, 2506
BcgI CGANNNNNNTGC 6 cut(s) 157, 191, 218, 252, 1911, 1945
BciT130I CCWGG 4 cut(s) 723, 891, 1068, 1565
BciVI GTATCC 2 cut(s) 1981, 2370
BclI TGATCA 3 cut(s) 24, 1105, 2284
BcoDI GTCTC 7 cut(s) 108, 461, 837, 1051, 1082, 1604, 1883
BcuI ACTAGT 1 cut(s) 2429
BfaI CTAG 8 cut(s) 996, 1176, 1355, 1436, 1722, 2334, 2430, 2495
BfmI CTRYAG 1 cut(s) 825
BfuI GTATCC 2 cut(s) 1981, 2370
BglII AGATCT 1 cut(s) 1438
BisI GCNGC 8 cut(s) 440, 822, 825, 2160, 2173, 2195, 2297, 2316
BlsI GCNGC 8 cut(s) 441, 823, 826, 2161, 2174, 2196, 2298, 2317
Bme1390I CCNGG 4 cut(s) 723, 891, 1068, 1565
Bme18I GGWCC 2 cut(s) 1463, 1908
BmgBI CACGTC 1 cut(s) 936
BmgT120I GGNCC 2 cut(s) 1463, 1908
BmiI GGNNCC 4 cut(s) 919, 1631, 1719, 2064
BmrFI CCNGG 4 cut(s) 723, 891, 1068, 1565
BmsI GCATC 7 cut(s) 109, 1479, 1510, 1707, 1909, 1931, 1942
BmtI GCTAGC 1 cut(s) 2498
BpiI GAAGAC 4 cut(s) 710, 786, 1718, 1844
BplI GAGNNNNNCTC 2 cut(s) 1238, 1270
BpmI CTGGAG 1 cut(s) 912
Bpu10I CCTNAGC 1 cut(s) 443
Bpu14I TTCGAA 1 cut(s) 1406
BpuEI CTTGAG 1 cut(s) 2561
Bsa29I ATCGAT 1 cut(s) 1515
BsaAI YACGTR 3 cut(s) 54, 943, 960
BsaHI GRCGYC 1 cut(s) 1276
BsaJI CCNNGG 4 cut(s) 658, 1596, 1974, 2499
BsaWI WCCGGW 4 cut(s) 914, 1094, 1910, 2052
BsaXI ACNNNNNCTCC 6 cut(s) 254, 284, 968, 998, 1818, 1848
Bsc4I CCNNNNNNNGG 3 cut(s) 110, 1597, 2059
Bse118I RCCGGY 3 cut(s) 1094, 1910, 2052
Bse1I ACTGG 4 cut(s) 50, 234, 1036, 1618
Bse3DI GCAATG 1 cut(s) 979
BseAI TCCGGA 1 cut(s) 914
BseBI CCWGG 4 cut(s) 723, 891, 1068, 1565
BseCI ATCGAT 1 cut(s) 1515
BseDI CCNNGG 4 cut(s) 658, 1596, 1974, 2499
BseGI GGATG 6 cut(s) 1030, 1494, 1761, 1955, 2002, 2418
BseLI CCNNNNNNNGG 3 cut(s) 110, 1597, 2059
BseMI GCAATG 1 cut(s) 979
BseMII CTCAG 4 cut(s) 345, 457, 1928, 2499
BseNI ACTGG 4 cut(s) 50, 234, 1036, 1618
BseXI GCAGC 7 cut(s) 426, 808, 811, 2171, 2206, 2308, 2327
Bsh1285I CGRYCG 1 cut(s) 108
BshFI GGCC 6 cut(s) 657, 726, 1284, 1802, 2341, 2565
BshNI GGYRCC 1 cut(s) 1629
BshTI ACCGGT 3 cut(s) 1094, 1910, 2052
BshVI ATCGAT 1 cut(s) 1515
BsiEI CGRYCG 1 cut(s) 108
BsiHKAI GWGCWC 3 cut(s) 318, 360, 1867
BsiSI CCGG 5 cut(s) 915, 1095, 1911, 2053, 2566
BslFI GGGAC 2 cut(s) 148, 922
BslI CCNNNNNNNGG 3 cut(s) 110, 1597, 2059
BsmAI GTCTC 7 cut(s) 108, 461, 837, 1051, 1082, 1604, 1883
BsmBI CGTCTC 2 cut(s) 108, 1082
BsmFI GGGAC 2 cut(s) 148, 922
BsmI GAATGC 1 cut(s) 1958
BsnI GGCC 6 cut(s) 657, 726, 1284, 1802, 2341, 2565
Bsp119I TTCGAA 1 cut(s) 1406
Bsp1286I GDGCHC 3 cut(s) 318, 360, 1867
Bsp13I TCCGGA 1 cut(s) 914
Bsp1407I TGTACA 1 cut(s) 1444
Bsp19I CCATGG 3 cut(s) 658, 1596, 2499
BspACI CCGC 9 cut(s) 166, 617, 696, 757, 1131, 1650, 1748, 1753, 2173
BspANI GGCC 6 cut(s) 657, 726, 1284, 1802, 2341, 2565
BspCNI CTCAG 4 cut(s) 346, 456, 1929, 2500
BspDI ATCGAT 1 cut(s) 1515
BspEI TCCGGA 1 cut(s) 914
BspHI TCATGA 2 cut(s) 835, 2107
BspLI GGNNCC 4 cut(s) 919, 1631, 1719, 2064
BspMAI CTGCAG 1 cut(s) 829
BspOI GCTAGC 1 cut(s) 2498
BspPI GGATC 6 cut(s) 16, 911, 1168, 2057, 2070, 2396
BspT104I TTCGAA 1 cut(s) 1406
BspT107I GGYRCC 1 cut(s) 1629
BsrBI CCGCTC 1 cut(s) 1753
BsrDI GCAATG 1 cut(s) 979
BsrFI RCCGGY 3 cut(s) 1094, 1910, 2052
BsrGI TGTACA 1 cut(s) 1444
BsrI ACTGG 4 cut(s) 50, 234, 1036, 1618
BssAI RCCGGY 3 cut(s) 1094, 1910, 2052
BssECI CCNNGG 4 cut(s) 658, 1596, 1974, 2499
BssNI GRCGYC 1 cut(s) 1276
BssSI CACGAG 2 cut(s) 98, 1417
BssT1I CCWWGG 4 cut(s) 658, 1596, 1974, 2499
Bst2BI CACGAG 2 cut(s) 98, 1417
Bst2UI CCWGG 4 cut(s) 723, 891, 1068, 1565
Bst4CI ACNGT 7 cut(s) 206, 484, 499, 1717, 1763, 1869, 2190
Bst6I CTCTTC 1 cut(s) 2034
BstACI GRCGYC 1 cut(s) 1276
BstAUI TGTACA 1 cut(s) 1444
BstBAI YACGTR 3 cut(s) 54, 943, 960
BstBI TTCGAA 1 cut(s) 1406
BstC8I GCNNGC 3 cut(s) 1016, 1196, 2496
BstDEI CTNAG 4 cut(s) 354, 443, 1937, 2508
BstDSI CCRYGG 3 cut(s) 658, 1596, 2499
BstF5I GGATG 6 cut(s) 1030, 1494, 1761, 1955, 2002, 2418
BstHHI GCGC 2 cut(s) 241, 675
BstMAI GTCTC 7 cut(s) 108, 461, 837, 1051, 1082, 1604, 1883
BstMCI CGRYCG 1 cut(s) 108
BstMWI GCNNNNNNNGC 2 cut(s) 296, 978
BstNI CCWGG 4 cut(s) 723, 891, 1068, 1565
BstNSI RCATGY 3 cut(s) 1064, 1792, 1970
BstSCI CCNGG 4 cut(s) 721, 889, 1066, 1563
BstSFI CTRYAG 1 cut(s) 825
BstSNI TACGTA 1 cut(s) 960
BstV1I GCAGC 7 cut(s) 426, 808, 811, 2171, 2206, 2308, 2327
BstV2I GAAGAC 4 cut(s) 710, 786, 1718, 1844
BstX2I RGATCY 3 cut(s) 1160, 1438, 2062
BstXI CCANNNNNNTGG 4 cut(s) 150, 462, 1266, 1739
BstYI RGATCY 3 cut(s) 1160, 1438, 2062
Bsu15I ATCGAT 1 cut(s) 1515
BsuI GTATCC 2 cut(s) 1981, 2370
BsuRI GGCC 6 cut(s) 657, 726, 1284, 1802, 2341, 2565
BsuTUI ATCGAT 1 cut(s) 1515
BtgI CCRYGG 3 cut(s) 658, 1596, 2499
BtgZI GCGATG 2 cut(s) 1647, 2049
BtrI CACGTC 1 cut(s) 936
BtsCI GGATG 6 cut(s) 1030, 1494, 1761, 1955, 2002, 2418
BtsI GCAGTG 2 cut(s) 834, 1645
BtsIMutI CAGTG 4 cut(s) 211, 241, 834, 1645
Cac8I GCNNGC 3 cut(s) 1016, 1196, 2496
CaiI CAGNNNCTG 1 cut(s) 2165
CciI TCATGA 2 cut(s) 835, 2107
CfoI GCGC 2 cut(s) 241, 675
Cfr10I RCCGGY 3 cut(s) 1094, 1910, 2052
Cfr13I GGNCC 2 cut(s) 1463, 1908
ClaI ATCGAT 1 cut(s) 1515
CseI GACGC 1 cut(s) 791
CsiI ACCWGGT 1 cut(s) 1563
CspAI ACCGGT 3 cut(s) 1094, 1910, 2052
DdeI CTNAG 4 cut(s) 354, 443, 1937, 2508
DraIII CACNNNGTG 1 cut(s) 1871
EaeI YGGCCR 2 cut(s) 1282, 1800
Eam1104I CTCTTC 1 cut(s) 2034
EarI CTCTTC 1 cut(s) 2034
EciI GGCGGA 1 cut(s) 1665
Ecl136II GAGCTC 1 cut(s) 316
Eco105I TACGTA 1 cut(s) 960
Eco130I CCWWGG 4 cut(s) 658, 1596, 1974, 2499
Eco24I GRGCYC 1 cut(s) 318
Eco47I GGWCC 2 cut(s) 1463, 1908
Eco53kI GAGCTC 1 cut(s) 316
Eco57I CTGAAG 1 cut(s) 2546
EcoICRI GAGCTC 1 cut(s) 316
EcoRI GAATTC 2 cut(s) 761, 1670
EcoRII CCWGG 4 cut(s) 721, 889, 1066, 1563
EcoT14I CCWWGG 4 cut(s) 658, 1596, 1974, 2499
EcoT22I ATGCAT 4 cut(s) 1494, 1790, 1924, 2018
EcoT38I GRGCYC 1 cut(s) 318
ErhI CCWWGG 4 cut(s) 658, 1596, 1974, 2499
Esp3I CGTCTC 2 cut(s) 108, 1082
FalI AAGNNNNNCTT 4 cut(s) 565, 597, 1149, 1181
FaqI GGGAC 2 cut(s) 148, 922
FauI CCCGC 1 cut(s) 750
FbaI TGATCA 3 cut(s) 24, 1105, 2284
FblI GTMKAC 1 cut(s) 1072
Fnu4HI GCNGC 8 cut(s) 440, 822, 825, 2160, 2173, 2195, 2297, 2316
FokI GGATG 6 cut(s) 1037, 1501, 1768, 1962, 2009, 2405
FriOI GRGCYC 1 cut(s) 318
Fsp4HI GCNGC 8 cut(s) 440, 822, 825, 2160, 2173, 2195, 2297, 2316
FspAI RTGCGCAY 1 cut(s) 240
FspBI CTAG 8 cut(s) 996, 1176, 1355, 1436, 1722, 2334, 2430, 2495
FspI TGCGCA 2 cut(s) 240, 674
GlaI GCGC 2 cut(s) 240, 674
GluI GCNGC 8 cut(s) 440, 822, 825, 2160, 2173, 2195, 2297, 2316
GsuI CTGGAG 1 cut(s) 912
HaeIII GGCC 6 cut(s) 657, 726, 1284, 1802, 2341, 2565
HapII CCGG 5 cut(s) 915, 1095, 1911, 2053, 2566
HgaI GACGC 1 cut(s) 791
HhaI GCGC 2 cut(s) 241, 675
Hin1I GRCGYC 1 cut(s) 1276
Hin6I GCGC 2 cut(s) 239, 673
HinP1I GCGC 2 cut(s) 239, 673
HincII GTYRAC 2 cut(s) 322, 1092
HindII GTYRAC 2 cut(s) 322, 1092
HindIII AAGCTT 3 cut(s) 297, 1148, 2552
HpaII CCGG 5 cut(s) 915, 1095, 1911, 2053, 2566
HphI GGTGA 6 cut(s) 878, 1145, 2178, 2234, 2395, 2560
Hpy188I TCNGA 9 cut(s) 29, 595, 863, 1453, 1467, 1676, 1703, 1938, 2509
HpyAV CCTTC 5 cut(s) 125, 567, 624, 1643, 2228
HpyCH4III ACNGT 7 cut(s) 206, 484, 499, 1717, 1763, 1869, 2190
HpyCH4IV ACGT 7 cut(s) 53, 935, 942, 959, 1075, 1276, 1577
HpyF10VI GCNNNNNNNGC 2 cut(s) 296, 978
HpyF3I CTNAG 4 cut(s) 354, 443, 1937, 2508
HpySE526I ACGT 7 cut(s) 53, 935, 942, 959, 1075, 1276, 1577
Hsp92I GRCGYC 1 cut(s) 1276
HspAI GCGC 2 cut(s) 239, 673
Kpn2I TCCGGA 1 cut(s) 914
Ksp22I TGATCA 3 cut(s) 24, 1105, 2284
LmnI GCTCC 7 cut(s) 517, 633, 917, 1750, 1978, 2278, 2293
Lsp1109I GCAGC 7 cut(s) 426, 808, 811, 2171, 2206, 2308, 2327
LweI GCATC 7 cut(s) 109, 1479, 1510, 1707, 1909, 1931, 1942
MabI ACCWGGT 1 cut(s) 1563
MaeI CTAG 8 cut(s) 996, 1176, 1355, 1436, 1722, 2334, 2430, 2495
MaeII ACGT 7 cut(s) 53, 935, 942, 959, 1075, 1276, 1577
MbiI CCGCTC 1 cut(s) 1753
MfeI CAATTG 1 cut(s) 2121
MflI RGATCY 3 cut(s) 1160, 1438, 2062
MhlI GDGCHC 3 cut(s) 318, 360, 1867
MlsI TGGCCA 2 cut(s) 1284, 1802
MluNI TGGCCA 2 cut(s) 1284, 1802
MlyI GAGTC 5 cut(s) 24, 272, 1352, 1885, 2513
MmeI TCCRAC 2 cut(s) 66, 240
Mox20I TGGCCA 2 cut(s) 1284, 1802
Mph1103I ATGCAT 4 cut(s) 1494, 1790, 1924, 2018
MroI TCCGGA 1 cut(s) 914
MroXI GAANNNNTTC 4 cut(s) 577, 1412, 1431, 2078
MscI TGGCCA 2 cut(s) 1284, 1802
MseI TTAA 4 cut(s) 309, 543, 1040, 1793
MslI CAYNNNNRTG 7 cut(s) 186, 711, 1125, 1131, 1290, 1452, 1598
Msp20I TGGCCA 2 cut(s) 1284, 1802
MspI CCGG 5 cut(s) 915, 1095, 1911, 2053, 2566
MspR9I CCNGG 4 cut(s) 723, 891, 1068, 1565
MunI CAATTG 1 cut(s) 2121
Mva1269I GAATGC 1 cut(s) 1958
MvaI CCWGG 4 cut(s) 723, 891, 1068, 1565
MwoI GCNNNNNNNGC 2 cut(s) 296, 978
NcoI CCATGG 3 cut(s) 658, 1596, 2499
NheI GCTAGC 1 cut(s) 2494
NlaIV GGNNCC 4 cut(s) 919, 1631, 1719, 2064
NmeAIII GCCGAG 1 cut(s) 87
NmuCI GTSAC 5 cut(s) 349, 855, 1640, 2383, 2432
NsbI TGCGCA 2 cut(s) 240, 674
NsiI ATGCAT 4 cut(s) 1494, 1790, 1924, 2018
NspI RCATGY 3 cut(s) 1064, 1792, 1970
NspV TTCGAA 1 cut(s) 1406
OliI CACNNNNGTG 2 cut(s) 1290, 1598
PagI TCATGA 2 cut(s) 835, 2107
PciI ACATGT 2 cut(s) 1060, 1966
PcsI WCGNNNNNNNCGW 4 cut(s) 59, 105, 114, 2538
PctI GAATGC 1 cut(s) 1958
PdmI GAANNNNTTC 4 cut(s) 577, 1412, 1431, 2078
PfeI GAWTC 8 cut(s) 911, 1250, 1478, 2183, 2201, 2344, 2467, 2516
PflMI CCANNNNNTGG 1 cut(s) 1597
PfoI TCCNGGA 1 cut(s) 889
PinAI ACCGGT 3 cut(s) 1094, 1910, 2052
PkrI GCNGC 8 cut(s) 441, 823, 826, 2161, 2174, 2196, 2298, 2317
Ple19I CGATCG 1 cut(s) 108
PleI GAGTC 5 cut(s) 24, 271, 1352, 1885, 2512
PpsI GAGTC 5 cut(s) 24, 271, 1352, 1885, 2512
Ppu21I YACGTR 3 cut(s) 54, 943, 960
PscI ACATGT 2 cut(s) 1060, 1966
Psp124BI GAGCTC 1 cut(s) 318
Psp6I CCWGG 4 cut(s) 721, 889, 1066, 1563
PspGI CCWGG 4 cut(s) 721, 889, 1066, 1563
PspN4I GGNNCC 4 cut(s) 919, 1631, 1719, 2064
PspPI GGNCC 2 cut(s) 1463, 1908
PsrI GAACNNNNNNTAC 2 cut(s) 2522, 2554
PstI CTGCAG 1 cut(s) 829
PstNI CAGNNNCTG 1 cut(s) 2165
PsuI RGATCY 3 cut(s) 1160, 1438, 2062
PvuI CGATCG 1 cut(s) 108
RseI CAYNNNNRTG 7 cut(s) 186, 711, 1125, 1131, 1290, 1452, 1598
SacI GAGCTC 1 cut(s) 318
SaqAI TTAA 4 cut(s) 309, 543, 1040, 1793
SatI GCNGC 8 cut(s) 440, 822, 825, 2160, 2173, 2195, 2297, 2316
Sau96I GGNCC 2 cut(s) 1463, 1908
SchI GAGTC 5 cut(s) 24, 272, 1352, 1885, 2513
ScrFI CCNGG 4 cut(s) 723, 891, 1068, 1565
SduI GDGCHC 3 cut(s) 318, 360, 1867
SexAI ACCWGGT 1 cut(s) 1563
SfaNI GCATC 7 cut(s) 109, 1479, 1510, 1707, 1909, 1931, 1942
SfcI CTRYAG 1 cut(s) 825
SfuI TTCGAA 1 cut(s) 1406
SinI GGWCC 2 cut(s) 1463, 1908
SmiMI CAYNNNNRTG 7 cut(s) 186, 711, 1125, 1131, 1290, 1452, 1598
SmlI CTYRAG 1 cut(s) 2576
SmoI CTYRAG 1 cut(s) 2576
SnaBI TACGTA 1 cut(s) 960
SpeI ACTAGT 1 cut(s) 2429
SsiI CCGC 9 cut(s) 166, 617, 696, 757, 1131, 1650, 1748, 1753, 2173
SspI AATATT 3 cut(s) 541, 991, 1432
SspMI CTAG 8 cut(s) 996, 1176, 1355, 1436, 1722, 2334, 2430, 2495
SstI GAGCTC 1 cut(s) 318
StyD4I CCNGG 4 cut(s) 721, 889, 1066, 1563
StyI CCWWGG 4 cut(s) 658, 1596, 1974, 2499
TaaI ACNGT 7 cut(s) 206, 484, 499, 1717, 1763, 1869, 2190
TaiI ACGT 7 cut(s) 56, 938, 945, 962, 1078, 1279, 1580
TaqI TCGA 8 cut(s) 108, 273, 346, 1245, 1406, 1476, 1515, 2133
TaqII GACCGA 1 cut(s) 167
TatI WGTACW 5 cut(s) 379, 1099, 1444, 1656, 2415
TauI GCSGC 1 cut(s) 2175
TfiI GAWTC 8 cut(s) 911, 1250, 1478, 2183, 2201, 2344, 2467, 2516
Tru1I TTAA 4 cut(s) 309, 543, 1040, 1793
Tru9I TTAA 4 cut(s) 309, 543, 1040, 1793
TscAI CASTG 4 cut(s) 211, 241, 834, 1645
TseFI GTSAC 5 cut(s) 349, 855, 1640, 2383, 2432
TseI GCWGC 7 cut(s) 439, 821, 824, 2159, 2194, 2296, 2315
Tsp45I GTSAC 5 cut(s) 349, 855, 1640, 2383, 2432
TspGWI ACGGA 4 cut(s) 28, 1037, 1543, 2451
TspRI CASTG 4 cut(s) 211, 241, 834, 1645
Van91I CCANNNNNTGG 1 cut(s) 1597
VpaK11BI GGWCC 2 cut(s) 1463, 1908
XapI RAATTY 5 cut(s) 761, 1670, 1817, 1883, 2078
XbaI TCTAGA 1 cut(s) 1435
XceI RCATGY 3 cut(s) 1064, 1792, 1970
XcmI CCANNNNNNNNNTGG 1 cut(s) 1902
XmiI GTMKAC 1 cut(s) 1072
XmnI GAANNNNTTC 4 cut(s) 577, 1412, 1431, 2078
XspI CTAG 8 cut(s) 996, 1176, 1355, 1436, 1722, 2334, 2430, 2495
ZraI GACGTC 1 cut(s) 1277
Zsp2I ATGCAT 4 cut(s) 1494, 1790, 1924, 2018
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.