RchiOBHm_Chr3g0448581

Peptidase inhibitor I9

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000278
Physical Location & Seq
Reverse (-)
766446 .. 771898
5453 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41601

Sequence Viewer

Length: 4116 bp
ATGAGGAATAACAATGGGATTCCTCTTCCTTGCATTTTCTCTGTTCTTTCATGTTTTCTGTTAACTGTACATGTCAACATGGCTTTGGCAGAGAGGTCTACTCATATTGTCCACATGGACAAGTCTCTCATGCCCAAGTCCTTCACTAGCCATCAACATTGGTACTCATCCATTGTTGATTCCTTCAAAACTGATCTTCACACCTCTTTGGATCGTAACAGGTCCATGCCATCCCTTTTATACACCTACGATAATGCCTTTCATGGTTTCTCTGCAGTTCTGTCCGCAGATGAATTGTTGAATTTAGAAAAGTCTCCAGCTTTTGTTTCTGCTTACAAGGATAAAACTGTCACAGTTGACACCACCCATACCACTACATTCCTCTCCCTCAACCCTTCCACTGGTTTGTTACCTGCTTCGAATTATGGTGAAGACATCATTGTTGGTGTCATTGATAGTGGGATCTGGCCAGAGAGCGATAGCTTCAGAGATGATGGTGAAACTCTCCGAGGACGTAGGCAAGGATATTGGCCGAACCTCCTTCGGCTTATCGGTCGTGGATTGTTTGTCATGGCAAGTTCTAGCGTCTCACATTTCAAGGTGGAGCAGTTTGATGGAAAGGGAAATTTCACTCTATGGCAAAGGAGGGTGAAGGACATCCTAGTTCAACAAGGTCTTGCAAAACCTTTGAAGGGAAAGGATGCGAAGCCGGTGAAGATGTCAGATGAAGATTGGGAAGAGTTGGAGTCCCGGTGTGTCAGCACTATTAGGCTTTATATTGCTGACAATATTATCAATAATGTTAATAATGTTGATTCGGCAACTCAGCTTTGGGAAAAAATGGAGAAGCTTCATCTTGGGAAAGGCTTGCAGACGAAGTTGAATCTAAAGCGGGATCTCTACAAGCTCAAGATGGGAGAAGGTGCGAGTCTCATGGAGCACATGAACGTGTTCAGAGGATTGGTAGATCAACTAGCAAAAGTTGATGTAAAGATTGAGGAAGAAGATCAAGCCCTTCTACTTCTTACCTCGCTTCCCGATTCGTATGAGAATATTATCACTACAATCTTGTATGGTAAGGATACATTAAAGATGGAGGAGGTGGAATCGACTCTCTTAAATCACGAGAAGCGGAGGAAAGCCGATGATTCCCAAATTTCCGTCTTTGTTGCTCAAGATCAGAATCGATGGGGAAGGTCAACTGCCAGGGGATCTAGTGGACATAGCAGGTCTAAATCCAGAGGCAGGGGAAAGGGCAAGCAGTGCTACAAGTGCAAGGAGTGGGGACACATAAGGCCGGATTGTCCCTTGTGGAAAGAGAAGGATGATAAGGGCTCAGATTGCTCCATGACTGGTATAGCACAAGCTTCAAATGATTTTGGAGAATTTCTCACAGTCTCCAAAGGTAATTACACTTGTTCTCAACGTGATTGGATATTGGATACTGGTTCTTCTCATCATCTTTGCTCCAGGAGAGAATATTTTGATACGTTCCAAGAAGTCAAAGGATTTGTGACTTGGGGTGATGGCACAAGGCGTTGCGTCATGGGTGTTGGAACAGTGAAGATCAAGATGTTTGATGGAGCGGTTCGTACTTTGGGTGATGTTGTATACGTCCCAAGGTTCCGAAGAAATTTAGTATCCTTGAGCCAGCTAGACTCCAAGGGCTGCAGGGTTTCATCAGCAGGGGGAGCTATGAAAATATCACGTGGCTGCATGGTCCTAATGAAGGGAGAAAAGTGTGGAAGTATGTTCCGTCTGATAGGAAAAACTCAGACCTCGAAGGTTGAAGGGAAACGGTGTGCACGAGGATGCAGGTACCTGAAGCGGGTCTCGTTTGCGAGCATAGCAGAGACTCCAGTGACAAGTTTCCAAGTTTTAGACGATGGTGAGAGGGTAAATCCTGCTAGGGAAGGGGTCAAGTCGAGGTCCCTCTTCAGAGTCAACAGGCATGTTGCTGGCATTGAAGAAAAACAAGAGTGTTTTTCTAATGGTTCCTTTCACACTTCCTTTATTGGAGGAGACAAGAAAGATGAAAACATGCAAGAAAACAAGGCAGCAAACAAGGAAAGTGAAAGCATGCAAGGGAAAAGAAGAAAAGGAGAAAGCATGCCGGCTTTGGAGAAAGAGCTGGAGAGAGAATGTGAGAGAGGGGGATATATATTCCCCATATCGGCAACATGGAGGGAGAGAGGGCAGAAGCACAAAGAGAGTAAGGAGAGAGCAGAAACTTTCTTTGTGAGCAACTCGGGTCTTCGTGAAACTCTCCGAGGACGTAGGCAAGGATATTGGCCGAACCTCGTTAAATCTTGCTGTCTGACCAAGAACATTCCAGCTAAGTGGAAGGGAAGATGTGAGGTTGGACAAGAGTTCAATGCCTCTTTGTGTAACAACAAATTAATAGGAGCAAGATACTTTAACAAGGGTGTGAGGGCTGCAAACCCCGGTGTCACACTTAGCATGAACTCTGCTAGAGACTCTGAAGGCCATGGGACTCACACATCCTCGACCGCTGCAGGGAACTTCGTGAATGGGGCATCTTATTTCGGTTATGCTAAAGGAACAGCTAGAGGTGTAGCACCACGTTCCAGGGTTGCCATGTACAAGGTCCTTTGGGATGAAGGTAGATATGCCTCTGATGTTCTAGCAGGCATGGACCAAGCTATTGCGGATGGTGTGGATGTTATTTCAATCTCACTGGGCTTTGATGACACGCCATTGTACGAGGATCCCGTAGCAATAGCATCCTTTGCCGCTATGGAGAAGAACGTAGTAGTTTCATCTTCAGCCGGAAATGAAGGCACAAAGGGCCTTGGGAAATTGCACAATGGCATCCCTTGGGCCCTGACTGTTGCAGCTGGCACGATTGACCGATCATTTGGTGGAACATTAAGTCTTGGTAATGGTTTAACCGTTCCTGGATTTACCTTGTTCCCAGAAAATGCTCTGATAAATAAGCTTCCACTGGTTTACAACAAGACATTATCAGCTTGCAACGACACAGAACTATTATATACTGGCCCCAGTGCAATCCTCATATGTGATGACATTTCGGATATCCGTGGTCAAATATCTCATATCATTGCTGCAGAGGTGCTTGGAGCTGTCTTTATTTCGAACGATCCTAATATTCATGAGTTAGGATATGTTGGAACTCCTATGGTGGTGGTAAGTCCAGATGATGGACCAGCTCTTTTCAAATATGCAAAAAGTGCTGACCCTATCGTTAGCATAACTTTCCAACAAACATTTGTTGGGACAAAGCCTGCACCGGCTGCTGCATTTTACTCTTCAAGAGGTCCTTCATCAAGTTATCCAGGTATCTTAAAGCCGGACATAATGGCACCGGGGTCATTAGTCTTGGCTGCTTGGCCTCCGAATGTAGCAGCTGCCCAAATTGGTGAAAATGTGATGTTACCAAGTGACTATAATTTGATATCTGGAACATCCATGTCTTGCCCTCATGCTTCAGGAGTAGCTGCTCTACTGAAAGGTGCACACCCAGAATGGAGTGCAGCTGCCATTAGGTCTGCTTTGGTCACCACAGCCAACCCATTAGACAATACTCAGAATCCAATTCGTGACAATGGTGACAATTTCAACTTTGCTTCACCTCTAGCTATGGGAGCAGGCCAGATTGATCCTAACAGAGCACTTGAACCTGGTTTGATATATGATGCAACCACTCAAGACTATATCAATCTCTTGTGCTCCACAAACTTTACCCGCAAGCAAATCTCAGCCATCACTAGATCACATGACTACGATTGTTCCAAGCCATCGGGTGATCTGAACTACCCATCGTTCGTCGCTTTGTATGGTCGACATCATCCAAAGAAATTAAGGGTTCAAAAGTTTCAGAGGACAGCAACAAATGTGGGAGATGCTGCAGCCAAGTACAAGGCTGTGGTAACAGCTCCAAAGGGTTCTACGGTAACAGTGTCGCCTGAGATACTGAAATTTGCAAATATATATGAAAAGCAAAACTACACTGTTACTATAAAGTATGAGGGGGACAAAAAGAATAGAAAGGTTTCTTTTGGTGAACTTGTTTGGGTCGAAGAAAATGGAAAATACAAAGTCAGGAGCCCCATTGTAGTGTCACCTCTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

1371

Amino Acids

150.87

Weight (kDa)

8.95

Isoelectric Point (pI)

35.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 34 - 119 7.3e-16 Peptidase inhibitor I9
Retrotran_gag_2 PF14223 220 - 383 8.2e-37 gag-polypeptide of LTR copia-type
Pol_BBD PF22936 478 - 557 1.2e-24 Pol polyprotein, beta-barrel domain
Peptidase_S8 PF00082 817 - 1189 2.7e-41 Subtilase family
fn3_6 PF17766 1263 - 1367 2.1e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000278)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G29785
fragaria_vesca FvH4_1g10046 FvH4_1g17351 FvH4_1g18441 FvH4_1g24222 FvH4_2g00141 FvH4_2g05931 FvH4_2g06671 FvH4_2g14030 FvH4_2g15291 FvH4_2g22611 FvH4_2g22612 FvH4_2g26380 FvH4_2g39202 FvH4_2g39202 FvH4_3g06691 FvH4_3g08580 FvH4_3g20502 FvH4_3g20503 FvH4_3g22421 FvH4_3g42580 FvH4_4g02061 FvH4_4g06794 FvH4_4g08661 FvH4_4g11771 FvH4_4g14601 FvH4_4g14721 FvH4_4g20921 FvH4_4g33601 FvH4_4g33602 FvH4_5g07492 FvH4_5g20321 FvH4_5g21591 FvH4_5g30343 FvH4_5g37875 FvH4_6g04372 FvH4_6g04373 FvH4_6g04373 FvH4_6g21377 FvH4_6g23760 FvH4_6g29471 FvH4_6g43142 FvH4_7g14351 FvH4_7g14352 FvH4_7g22582 FvH4_7g22583 FvH4_7g22584
malus_domestica MD00G1179400.v1.1 MD01G1170500.v1.1 MD03G1286700.v1.1 MD04G1054600.v1.1 MD04G1071700.v1.1 MD04G1193900.v1.1 MD08G1054300.v1.1 MD08G1217500.v1.1 MD09G1034600.v1.1 MD09G1098100.v1.1 MD10G1018300.v1.1 MD10G1220000.v1.1 MD10G1250300.v1.1 MD12G1014400.v1.1 MD15G1021000.v1.1 MD16G1112200.v1.1
prunus_persica Prupe.1G188900_v2.0.a1 Prupe.1G258000_v2.0.a1 Prupe.4G233400_v2.0.a1
pyrus_communis pycom01g10100 pycom02g01340 pycom03g19700 pycom05g01320 pycom07g16000 pycom07g16520 pycom11g26500 pycom13g09380 pycom13g13960 pycom14g08180 pycom14g16810 pycom16g09590
rosa_chinensis RchiOBHm_Chr1g0319891 RchiOBHm_Chr1g0333711 RchiOBHm_Chr2g0115331 RchiOBHm_Chr2g0143411 RchiOBHm_Chr2g0167631 RchiOBHm_Chr3g0448581 RchiOBHm_Chr3g0494991 RchiOBHm_Chr4g0442471 RchiOBHm_Chr6g0262311 RchiOBHm_Chr6g0301831 RchiOBHm_Chr6g0301841 RchiOBHm_Chr7g0183451
rosa_roxburghii Rroxscaffold_1G00039280 Rroxscaffold_1G00047670 Rroxscaffold_1G00066330 Rroxscaffold_2G00107510 Rroxscaffold_3G00255410 Rroxscaffold_4G00301460 Rroxscaffold_5G00352730 Rroxscaffold_7G00166350 Rroxscaffold_7G00166360 Rroxscaffold_7G00203820
rosa_rugosa Rorug03G0240500 Rorug05G0191700 Rorug06G0310200
rosa_samantha Rh2BG287000 Rh4CG073500 Rh4CG176200 Rh4CG191400 Rh5BG356100 Rh5BG434100 Rh6AG110600 Rh6AG421900 Rh6AG422000 Rh6AG422100 Rh6BG428600 Rh6BG428700 Rh6BG428800 Rh6CG435200 Rh6CG435300 Rh6CG435400 Rh6DG422000 Rh6DG422100 Rh6DG422200 Rh7BG190500 Rh7CG172800 Rh7CG225200
rosa_wichuraiana Rw2G044060 Rw6G036540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 421, 1218, 1806
Acc65I GGTACC 1 cut(s) 1818
AccB1I GGYRCC 2 cut(s) 1818, 3343
AccB7I CCANNNNNTGG 1 cut(s) 3182
AccBSI CCGCTC 1 cut(s) 1586
AccI GTMKAC 3 cut(s) 98, 1611, 3823
AciI CCGC 9 cut(s) 285, 892, 1132, 1586, 1828, 2511, 2669, 2754, 3727
AclWI GGATC 8 cut(s) 219, 470, 903, 1219, 2723, 2736, 3116, 3635
AcoI YGGCCR 3 cut(s) 467, 530, 2291
AcsI RAATTY 6 cut(s) 301, 625, 1155, 1385, 1633, 3959
AcuI CTGAAG 6 cut(s) 469, 1844, 1921, 2502, 2769, 3453
AcvI CACGTG 1 cut(s) 1709
AfaI GTAC 7 cut(s) 69, 164, 1594, 1820, 2603, 2723, 3899
AfiI CCNNNNNNNGG 8 cut(s) 401, 692, 1245, 1729, 1828, 2173, 2517, 3182
AflIII ACRYGT 2 cut(s) 70, 948
AjnI CCWGG 6 cut(s) 1205, 1469, 2588, 2917, 3316, 3661
Alw21I GWGCWC 5 cut(s) 942, 1807, 3499, 3655, 3713
Alw26I GTCTC 9 cut(s) 129, 318, 592, 935, 1402, 1837, 1847, 2016, 2469
Alw44I GTGCAC 2 cut(s) 1803, 3495
AlwI GGATC 8 cut(s) 219, 470, 903, 1219, 2723, 2736, 3116, 3635
Ama87I CYCGRG 1 cut(s) 2248
ApaI GGGCCC 1 cut(s) 2845
ApaLI GTGCAC 2 cut(s) 1803, 3495
ApoI RAATTY 6 cut(s) 301, 625, 1155, 1385, 1633, 3959
ArsI GACNNNNNNTTYG 2 cut(s) 2861, 2893
AseI ATTAAT 1 cut(s) 2399
Asp700I GAANNNNTTC 2 cut(s) 950, 4033
Asp718I GGTACC 1 cut(s) 1818
AsuC2I CCSGG 3 cut(s) 751, 2445, 3348
AsuII TTCGAA 2 cut(s) 419, 3116
AvaI CYCGRG 1 cut(s) 2248
AvaII GGWCC 7 cut(s) 222, 1720, 1929, 2608, 2656, 3185, 3299
BaeGI GKGCMC 3 cut(s) 1807, 2845, 3499
BalI TGGCCA 1 cut(s) 469
BamHI GGATCC 1 cut(s) 2728
BanI GGYRCC 2 cut(s) 1818, 3343
BanII GRGCYC 3 cut(s) 1337, 2845, 4091
BauI CACGAG 2 cut(s) 1124, 1806
BbrPI CACGTG 1 cut(s) 1709
BbsI GAAGAC 2 cut(s) 438, 2246
Bbv12I GWGCWC 5 cut(s) 942, 1807, 3499, 3655, 3713
BciT130I CCWGG 6 cut(s) 1207, 1471, 2590, 2919, 3318, 3663
BciVI GTATCC 3 cut(s) 1075, 1435, 1651
BcnI CCSGG 3 cut(s) 751, 2445, 3348
BcoDI GTCTC 9 cut(s) 129, 318, 592, 935, 1402, 1837, 1847, 2016, 2469
BfmI CTRYAG 5 cut(s) 273, 1669, 2514, 3087, 3888
BfuAI ACCTGC 3 cut(s) 421, 1218, 1806
BfuI GTATCC 3 cut(s) 1075, 1435, 1651
Bme1390I CCNGG 9 cut(s) 751, 1207, 1471, 2445, 2590, 2919, 3318, 3348, 3663
Bme18I GGWCC 7 cut(s) 222, 1720, 1929, 2608, 2656, 3185, 3299
BmeT110I CYCGRG 1 cut(s) 2248
BmiI GGNNCC 9 cut(s) 1625, 1820, 1931, 1996, 2730, 2843, 3022, 3345, 4088
BmrFI CCNGG 9 cut(s) 751, 1207, 1471, 2445, 2590, 2919, 3318, 3348, 3663
BmrI ACTGGG 2 cut(s) 2708, 3018
BmsI GCATC 7 cut(s) 691, 1802, 2546, 2753, 2841, 3667, 3874
BmuI ACTGGG 2 cut(s) 2708, 3018
BpiI GAAGAC 2 cut(s) 438, 2246
BplI GAGNNNNNCTC 4 cut(s) 85, 117, 1374, 1406
BpmI CTGGAG 4 cut(s) 300, 1453, 1842, 2153
Bpu14I TTCGAA 2 cut(s) 419, 3116
BpuEI CTTGAG 4 cut(s) 893, 1158, 1666, 3672
BpuMI CCSGG 3 cut(s) 751, 2445, 3348
Bsa29I ATCGAT 1 cut(s) 1186
BsaAI YACGTR 1 cut(s) 1709
BsaBI GATNNNNATC 1 cut(s) 1182
BsaI GGTCTC 1 cut(s) 1837
BsaXI ACNNNNNCTCC 2 cut(s) 737, 767
Bsc4I CCNNNNNNNGG 8 cut(s) 401, 692, 1245, 1729, 1828, 2173, 2517, 3182
Bse118I RCCGGY 3 cut(s) 709, 2113, 3271
Bse1I ACTGG 8 cut(s) 406, 1357, 1450, 1859, 2703, 2970, 3022, 3024
Bse3DI GCAATG 1 cut(s) 3081
Bse8I GATNNNNATC 1 cut(s) 1182
BseBI CCWGG 6 cut(s) 1207, 1471, 2590, 2919, 3318, 3663
BseCI ATCGAT 1 cut(s) 1186
BseJI GATNNNNATC 1 cut(s) 1182
BseLI CCNNNNNNNGG 8 cut(s) 401, 692, 1245, 1729, 1828, 2173, 2517, 3182
BseMI GCAATG 1 cut(s) 3081
BseMII CTCAG 6 cut(s) 839, 1350, 1787, 3581, 3753, 3939
BseNI ACTGG 8 cut(s) 406, 1357, 1450, 1859, 2703, 2970, 3022, 3024
BseRI GAGGAG 2 cut(s) 1112, 2034
BseSI GKGCMC 3 cut(s) 1807, 2845, 3499
BsgI GTGCAG 2 cut(s) 3252, 3534
Bsh1285I CGRYCG 2 cut(s) 556, 2511
BshNI GGYRCC 2 cut(s) 1818, 3343
BshVI ATCGAT 1 cut(s) 1186
BsiEI CGRYCG 2 cut(s) 556, 2511
BsiHKAI GWGCWC 5 cut(s) 942, 1807, 3499, 3655, 3713
BsiHKCI CYCGRG 1 cut(s) 2248
BsiSI CCGG 9 cut(s) 710, 751, 1298, 2114, 2445, 2790, 3272, 3332, 3347
BslFI GGGAC 8 cut(s) 733, 1290, 1299, 1601, 1915, 2506, 3271, 4028
BslI CCNNNNNNNGG 8 cut(s) 401, 692, 1245, 1729, 1828, 2173, 2517, 3182
BsmAI GTCTC 9 cut(s) 129, 318, 592, 935, 1402, 1837, 1847, 2016, 2469
BsmBI CGTCTC 1 cut(s) 592
BsmFI GGGAC 8 cut(s) 733, 1290, 1299, 1601, 1915, 2506, 3271, 4028
Bso31I GGTCTC 1 cut(s) 1837
BsoBI CYCGRG 1 cut(s) 2248
Bsp119I TTCGAA 2 cut(s) 419, 3116
Bsp120I GGGCCC 1 cut(s) 2841
Bsp1286I GDGCHC 8 cut(s) 942, 1337, 1807, 2845, 3499, 3655, 3713, 4091
Bsp1407I TGTACA 2 cut(s) 67, 2601
Bsp19I CCATGG 1 cut(s) 2488
BspACI CCGC 9 cut(s) 285, 892, 1132, 1586, 1828, 2511, 2669, 2754, 3727
BspCNI CTCAG 6 cut(s) 838, 1349, 1786, 3580, 3752, 3940
BspDI ATCGAT 1 cut(s) 1186
BspHI TCATGA 1 cut(s) 3133
BspLI GGNNCC 9 cut(s) 1625, 1820, 1931, 1996, 2730, 2843, 3022, 3345, 4088
BspMAI CTGCAG 5 cut(s) 277, 1673, 2518, 3091, 3892
BspMI ACCTGC 3 cut(s) 421, 1218, 1806
BspPI GGATC 8 cut(s) 219, 470, 903, 1219, 2723, 2736, 3116, 3635
BspT104I TTCGAA 2 cut(s) 419, 3116
BspT107I GGYRCC 2 cut(s) 1818, 3343
BspTNI GGTCTC 1 cut(s) 1837
BsrBI CCGCTC 1 cut(s) 1586
BsrDI GCAATG 1 cut(s) 3081
BsrFI RCCGGY 3 cut(s) 709, 2113, 3271
BsrGI TGTACA 2 cut(s) 67, 2601
BsrI ACTGG 8 cut(s) 406, 1357, 1450, 1859, 2703, 2970, 3022, 3024
BssAI RCCGGY 3 cut(s) 709, 2113, 3271
BssNAI GTATAC 1 cut(s) 1612
BssSI CACGAG 2 cut(s) 1124, 1806
BssT1I CCWWGG 5 cut(s) 1619, 1662, 2488, 2812, 2837
Bst1107I GTATAC 1 cut(s) 1612
Bst2BI CACGAG 2 cut(s) 1124, 1806
Bst2UI CCWGG 6 cut(s) 1207, 1471, 2590, 2919, 3318, 3663
Bst6I CTCTTC 4 cut(s) 30, 732, 1940, 3295
BstAPI GCANNNNNTGC 4 cut(s) 1263, 2750, 3212, 3275
BstAUI TGTACA 2 cut(s) 67, 2601
BstBAI YACGTR 1 cut(s) 1709
BstBI TTCGAA 2 cut(s) 419, 3116
BstDEI CTNAG 8 cut(s) 825, 1336, 1773, 2337, 2456, 3567, 3739, 3948
BstDSI CCRYGG 2 cut(s) 2488, 3061
BstEII GGTNACC 1 cut(s) 3538
BstENI CCTNNNNNAGG 1 cut(s) 1727
BstMAI GTCTC 9 cut(s) 129, 318, 592, 935, 1402, 1837, 1847, 2016, 2469
BstMCI CGRYCG 2 cut(s) 556, 2511
BstNI CCWGG 6 cut(s) 1207, 1471, 2590, 2919, 3318, 3663
BstNSI RCATGY 5 cut(s) 74, 1955, 2044, 2083, 2113
BstPI GGTNACC 1 cut(s) 3538
BstSCI CCNGG 9 cut(s) 749, 1205, 1469, 2443, 2588, 2917, 3316, 3346, 3661
BstSFI CTRYAG 5 cut(s) 273, 1669, 2514, 3087, 3888
BstSLI GKGCMC 3 cut(s) 1807, 2845, 3499
BstV2I GAAGAC 2 cut(s) 438, 2246
BstX2I RGATCY 4 cut(s) 462, 895, 1211, 2728
BstXI CCANNNNNNTGG 1 cut(s) 2340
BstYI RGATCY 4 cut(s) 462, 895, 1211, 2728
BstZ17I GTATAC 1 cut(s) 1612
Bsu15I ATCGAT 1 cut(s) 1186
BsuI GTATCC 3 cut(s) 1075, 1435, 1651
BsuTUI ATCGAT 1 cut(s) 1186
BtgI CCRYGG 2 cut(s) 2488, 3061
BtsI GCAGTG 1 cut(s) 1268
BtsIMutI CAGTG 9 cut(s) 399, 1268, 1566, 1866, 2696, 2963, 3031, 3945, 3990
BveI ACCTGC 3 cut(s) 421, 1218, 1806
CciI TCATGA 1 cut(s) 3133
Cfr10I RCCGGY 3 cut(s) 709, 2113, 3271
ClaI ATCGAT 1 cut(s) 1186
CseI GACGC 2 cut(s) 574, 1531
CsiI ACCWGGT 1 cut(s) 3661
Csp6I GTAC 7 cut(s) 68, 163, 1593, 1819, 2602, 2722, 3898
CspCI CAANNNNNGTGG 2 cut(s) 3858, 3893
CviQI GTAC 7 cut(s) 68, 163, 1593, 1819, 2602, 2722, 3898
DdeI CTNAG 8 cut(s) 825, 1336, 1773, 2337, 2456, 3567, 3739, 3948
EaeI YGGCCR 3 cut(s) 467, 530, 2291
Eam1104I CTCTTC 4 cut(s) 30, 732, 1940, 3295
EarI CTCTTC 4 cut(s) 30, 732, 1940, 3295
Eco130I CCWWGG 5 cut(s) 1619, 1662, 2488, 2812, 2837
Eco24I GRGCYC 3 cut(s) 1337, 2845, 4091
Eco31I GGTCTC 1 cut(s) 1837
Eco32I GATATC 2 cut(s) 3058, 3438
Eco47I GGWCC 7 cut(s) 222, 1720, 1929, 2608, 2656, 3185, 3299
Eco57I CTGAAG 6 cut(s) 469, 1844, 1921, 2502, 2769, 3453
Eco72I CACGTG 1 cut(s) 1709
Eco88I CYCGRG 1 cut(s) 2248
Eco91I GGTNACC 1 cut(s) 3538
EcoNI CCTNNNNNAGG 1 cut(s) 1727
EcoO109I RGGNCCY 5 cut(s) 1929, 2608, 2809, 2842, 3299
EcoO65I GGTNACC 1 cut(s) 3538
EcoRII CCWGG 6 cut(s) 1205, 1469, 2588, 2917, 3316, 3661
EcoRV GATATC 2 cut(s) 3058, 3438
EcoT14I CCWWGG 5 cut(s) 1619, 1662, 2488, 2812, 2837
EcoT38I GRGCYC 3 cut(s) 1337, 2845, 4091
ErhI CCWWGG 5 cut(s) 1619, 1662, 2488, 2812, 2837
Esp3I CGTCTC 1 cut(s) 592
FalI AAGNNNNNCTT 2 cut(s) 3286, 3318
FaqI GGGAC 8 cut(s) 733, 1290, 1299, 1601, 1915, 2506, 3271, 4028
FauI CCCGC 3 cut(s) 885, 1821, 3734
FauNDI CATATG 1 cut(s) 3038
FblI GTMKAC 3 cut(s) 98, 1611, 3823
FriOI GRGCYC 3 cut(s) 1337, 2845, 4091
GsuI CTGGAG 4 cut(s) 300, 1453, 1842, 2153
HapII CCGG 9 cut(s) 710, 751, 1298, 2114, 2445, 2790, 3272, 3332, 3347
HgaI GACGC 2 cut(s) 574, 1531
HincII GTYRAC 6 cut(s) 63, 76, 358, 1200, 1945, 3824
HindII GTYRAC 6 cut(s) 63, 76, 358, 1200, 1945, 3824
HindIII AAGCTT 3 cut(s) 848, 1365, 2957
HpaI GTTAAC 1 cut(s) 63
HpaII CCGG 9 cut(s) 710, 751, 1298, 2114, 2445, 2790, 3272, 3332, 3347
Hpy99I CGWCG 1 cut(s) 3812
HpyCH4IV ACGT 9 cut(s) 514, 948, 1426, 1490, 1614, 1708, 2275, 2584, 2769
HpyF3I CTNAG 8 cut(s) 825, 1336, 1773, 2337, 2456, 3567, 3739, 3948
HpySE526I ACGT 9 cut(s) 514, 948, 1426, 1490, 1614, 1708, 2275, 2584, 2769
KpnI GGTACC 1 cut(s) 1822
KroI GCCGGC 1 cut(s) 2113
KroNI GCCGGC 1 cut(s) 2115
KspAI GTTAAC 1 cut(s) 63
LweI GCATC 7 cut(s) 691, 1802, 2546, 2753, 2841, 3667, 3874
MabI ACCWGGT 1 cut(s) 3661
MaeII ACGT 9 cut(s) 514, 948, 1426, 1490, 1614, 1708, 2275, 2584, 2769
MbiI CCGCTC 1 cut(s) 1586
MflI RGATCY 4 cut(s) 462, 895, 1211, 2728
MhlI GDGCHC 8 cut(s) 942, 1337, 1807, 2845, 3499, 3655, 3713, 4091
MlsI TGGCCA 1 cut(s) 469
MluNI TGGCCA 1 cut(s) 469
MlyI GAGTC 8 cut(s) 755, 937, 1105, 1652, 1849, 1950, 2471, 2488
MmeI TCCRAC 5 cut(s) 723, 1534, 2341, 3130, 3265
Mox20I TGGCCA 1 cut(s) 469
MroNI GCCGGC 1 cut(s) 2113
MroXI GAANNNNTTC 2 cut(s) 950, 4033
MscI TGGCCA 1 cut(s) 469
MslI CAYNNNNRTG 3 cut(s) 947, 1810, 4097
Msp20I TGGCCA 1 cut(s) 469
MspA1I CMGCKG 4 cut(s) 2513, 2858, 3389, 3518
MspI CCGG 9 cut(s) 710, 751, 1298, 2114, 2445, 2790, 3272, 3332, 3347
MspR9I CCNGG 9 cut(s) 751, 1207, 1471, 2445, 2590, 2919, 3318, 3348, 3663
MvaI CCWGG 6 cut(s) 1207, 1471, 2590, 2919, 3318, 3663
NaeI GCCGGC 1 cut(s) 2115
NciI CCSGG 3 cut(s) 751, 2445, 3348
NcoI CCATGG 1 cut(s) 2488
NdeI CATATG 1 cut(s) 3038
NgoMIV GCCGGC 1 cut(s) 2113
NlaIV GGNNCC 9 cut(s) 1625, 1820, 1931, 1996, 2730, 2843, 3022, 3345, 4088
NmuCI GTSAC 9 cut(s) 349, 1513, 1861, 2449, 3422, 3538, 3581, 3590, 4101
NspI RCATGY 5 cut(s) 74, 1955, 2044, 2083, 2113
NspV TTCGAA 2 cut(s) 419, 3116
PaeI GCATGC 2 cut(s) 2083, 2113
PagI TCATGA 1 cut(s) 3133
PciI ACATGT 1 cut(s) 70
PcsI WCGNNNNNNNCGW 2 cut(s) 1804, 2730
PdiI GCCGGC 1 cut(s) 2115
PdmI GAANNNNTTC 2 cut(s) 950, 4033
PfeI GAWTC 9 cut(s) 19, 179, 815, 883, 1040, 1106, 1148, 1183, 3571
PflMI CCANNNNNTGG 1 cut(s) 3182
PfoI TCCNGGA 2 cut(s) 1469, 2917
PleI GAGTC 8 cut(s) 754, 936, 1105, 1652, 1849, 1949, 2471, 2488
PmaCI CACGTG 1 cut(s) 1709
PmlI CACGTG 1 cut(s) 1709
PpsI GAGTC 8 cut(s) 754, 936, 1105, 1652, 1849, 1949, 2471, 2488
Ppu21I YACGTR 1 cut(s) 1709
PpuMI RGGWCCY 3 cut(s) 1929, 2608, 3299
PscI ACATGT 1 cut(s) 70
PshBI ATTAAT 1 cut(s) 2399
Psp5II RGGWCCY 3 cut(s) 1929, 2608, 3299
Psp6I CCWGG 6 cut(s) 1205, 1469, 2588, 2917, 3316, 3661
PspCI CACGTG 1 cut(s) 1709
PspEI GGTNACC 1 cut(s) 3538
PspGI CCWGG 6 cut(s) 1205, 1469, 2588, 2917, 3316, 3661
PspN4I GGNNCC 9 cut(s) 1625, 1820, 1931, 1996, 2730, 2843, 3022, 3345, 4088
PspOMI GGGCCC 1 cut(s) 2841
PspPPI RGGWCCY 3 cut(s) 1929, 2608, 3299
PstI CTGCAG 5 cut(s) 277, 1673, 2518, 3091, 3892
PsuI RGATCY 4 cut(s) 462, 895, 1211, 2728
PvuII CAGCTG 3 cut(s) 2858, 3389, 3518
RsaI GTAC 7 cut(s) 69, 164, 1594, 1820, 2603, 2723, 3899
RsaNI GTAC 7 cut(s) 68, 163, 1593, 1819, 2602, 2722, 3898
RseI CAYNNNNRTG 3 cut(s) 947, 1810, 4097
SalI GTCGAC 1 cut(s) 3822
SchI GAGTC 8 cut(s) 755, 937, 1105, 1652, 1849, 1950, 2471, 2488
ScrFI CCNGG 9 cut(s) 751, 1207, 1471, 2445, 2590, 2919, 3318, 3348, 3663
SduI GDGCHC 8 cut(s) 942, 1337, 1807, 2845, 3499, 3655, 3713, 4091
SexAI ACCWGGT 1 cut(s) 3661
SfaNI GCATC 7 cut(s) 691, 1802, 2546, 2753, 2841, 3667, 3874
SfcI CTRYAG 5 cut(s) 273, 1669, 2514, 3087, 3888
SfuI TTCGAA 2 cut(s) 419, 3116
SinI GGWCC 7 cut(s) 222, 1720, 1929, 2608, 2656, 3185, 3299
SmiMI CAYNNNNRTG 3 cut(s) 947, 1810, 4097
SmlI CTYRAG 4 cut(s) 908, 1173, 1645, 3687
SmoI CTYRAG 4 cut(s) 908, 1173, 1645, 3687
SphI GCATGC 2 cut(s) 2083, 2113
SsiI CCGC 9 cut(s) 285, 892, 1132, 1586, 1828, 2511, 2669, 2754, 3727
SspI AATATT 4 cut(s) 790, 1054, 1481, 3130
StyD4I CCNGG 9 cut(s) 749, 1205, 1469, 2443, 2588, 2917, 3316, 3346, 3661
StyI CCWWGG 5 cut(s) 1619, 1662, 2488, 2812, 2837
TaiI ACGT 9 cut(s) 517, 951, 1429, 1493, 1617, 1711, 2278, 2587, 2772
TaqI TCGA 9 cut(s) 419, 1109, 1186, 1781, 1925, 2507, 3116, 3823, 4059
TaqII GACCGA 2 cut(s) 542, 2886
TatI WGTACW 3 cut(s) 67, 2601, 3897
TauI GCSGC 1 cut(s) 2756
TfiI GAWTC 9 cut(s) 19, 179, 815, 883, 1040, 1106, 1148, 1183, 3571
TscAI CASTG 9 cut(s) 406, 1268, 1566, 1866, 2703, 2970, 3031, 3945, 3997
TseFI GTSAC 9 cut(s) 349, 1513, 1861, 2449, 3422, 3538, 3581, 3590, 4101
Tsp45I GTSAC 9 cut(s) 349, 1513, 1861, 2449, 3422, 3538, 3581, 3590, 4101
TspGWI ACGGA 3 cut(s) 1150, 1745, 3050
TspRI CASTG 9 cut(s) 406, 1268, 1566, 1866, 2703, 2970, 3031, 3945, 3997
Van91I CCANNNNNTGG 1 cut(s) 3182
VneI GTGCAC 2 cut(s) 1803, 3495
VpaK11BI GGWCC 7 cut(s) 222, 1720, 1929, 2608, 2656, 3185, 3299
VspI ATTAAT 1 cut(s) 2399
XagI CCTNNNNNAGG 1 cut(s) 1727
XapI RAATTY 6 cut(s) 301, 625, 1155, 1385, 1633, 3959
XceI RCATGY 5 cut(s) 74, 1955, 2044, 2083, 2113
XmiI GTMKAC 3 cut(s) 98, 1611, 3823
XmnI GAANNNNTTC 2 cut(s) 950, 4033
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.