FvH4_2g05931

disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000278
Physical Location & Seq
Forward (+)
4918262 .. 4922636
4375 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g05931.t1

Sequence Viewer

Length: 3714 bp
ATGGTAGACAGCAAAGACATGGTCGAAGCAGAAGATGTGAAATCGTTCATTAAGCCCTTAAATGGCAAGGATGACTTCACGCTTTGGCGGAGGAGGATGAAGAGTGTCCTGATTCTACAAGATCTGTACGAGGCTGTTCTCGGGATAGAGAATAAGGCAGTTGATATGGCAGATAAGGGTCCTATTGGTGAGAATATGGACAGCAACAAGACATTGGACAATGTCAAGGAGACTGACGCAGATAAATTCTTCTTGAAAGAAAAACTCTATAGTCTTCGGATGGAAGAAGGCAGGGATCTGCAGGAACATCTGAATAAATTTCAGATTTGTGTTGCCAATTTATCAGAGGTGGATATGCAATATGAAGAAGAGGATAAAGGACTTATGCTGCTAACATCTTTACCAGCTTCATACCAGCAGTTCATCACCACACTCGTTTCCGGTAAGGATTCTCTCAAGTACGATGAGAGTACCGAAGCTATTCAATCTTATATTAAGACGAGTAGCGAGACCGAGAGCTCTCAAGGAGGCCTTCAAGTCAAGGGCAAGGAGACAAGTCAGTTGACCAGTAAAAAGGATAAATCTAGTAACAAGGGTACAACAAAATCCAAGAAGAAGTACAAGGAAGGTTGCTTCAAATGTGATGCTGCAGACCACTTGAAAAGAAACTACAAAGAAGGGAGGATGGAAGCACAGGTGACGGCAAGGAGTTCAAGTACAGCCAACATAGTCATCAGAAAAGATGAAGATGATTGTGAACTTCTTGTGGTAACGTCCAGCTCCAACGCTTTCAGAGATTGGATTTTGGATACCGGTTGTACATTCCACATGTGTGCAATCCGAGAATGGTTTGACACATTTGAGGAGAGTAGCAGCGGAGAAGTTTTCCGGGGAGATGACTCACAGTGTAGGATCCTAGGTATTGGATCAGTGAAAATCAGAATGCATGACAGAGTTGTTAGAAAGCTGGAGAATGTCAGATATATTCCTAAGTTAAGGAAGAACTTGATATCTTTGGGTACTTTGGACAAGGCCGGTTATAGGTATAGCTCTAAAGAAGGAAGACTAAAAGTTATCAAGGGATCACTTGTTGTGATGAAGGGCGACATTCAACCTAACAACGTGTACAAGCTTCAAGGGACTACCATAATAGGTGGAGTTGCAATGTCTACTGAGGTAAATGACAAGACTGAGCAATGGCAGCAAGGCCTACGGCGCATGAGTCAAAGAGGAATGCAGGAACTCTACAAGGAAAGTAAGGCAGAAGGTGTTGGTGTTTGCAAACTGAACTTCTGCAAGAATGGTACTCTTGGGAAGCAGAAGGTGGCATTCAAGTTGGTAGATTGTGAGAACAAAAGCAAACGATTGCTGGATTATATTCACTTAGAAGTATGGAGCGCAACACAAGTAAGGCGTATGGGAGGCTTCAAGTACCTTGTTACTTTTCTAGATGATTTTTCAAGGAAGATTTGGGCCTACTTTATGAAGGAGAAATCCGAGGTTCTCTTCAAGGTCAAGAAAGGGAAAAGCAAAGTAGGAAATCTAGAAGTTAGAAAAATCAAATATTTGAGAAGCAATGGTGGCAGTGAATATAGAATGAAAAGGTTCTTACAGTTTTACAAGGATGAAATCACAAGGTGCATCACGGTGAAGGATAATCTCCAGGAAAACGAGGCTGCTAGAAGGATGAAGAAAACTTTTTTTGAGAAAGAAAGAGGCATGCCACTGCATGCTGGGTTACCAGACACGTTTTGGAAAGAAGCAGTTAACCATGCGTGTTATTTGGTTAATCGGTCACCATCAAAGACAAGTAAGGTGAAGACGAAGACTGATGTTGGTGTTGTAGAAGAAGCAACAGAAGTTCCTCTAACCAAAGAGATCATGGAAGAGACTCCAGCTCAGGTGGAGCAAATTGAGCAGGTGGAGCATGATAAAGGGGTTATCGAGGTAGAAGTCTTAGAGCGGCAACAACAGTCACTAATACAATCTCAGGTGGAGCAATTAGCACTGCGAACCTACGACTCAGTTCGTCAGTCGCTTGAGCAGGAGTTCCAGAGAAGCATAGCATTGGACAAGTATGCTCTAAGTTTCAGTCCAGGAGATCCAACCACGTATCAGGAAGCTATAGCAAAATATATACGGGAAAGTTGGATGGGAGCTATGTTGGAACAGAAGAAGAAGTCCATACCAAAGGACTTGGTTCAGGAGTCGGTTCCTAAACCCAAAGAAGGAAATCTGGTTGGCTGTAAATGGGTTTATAGGAAAAGGGAAGAAATGCATGATAAGGAAGCCATGAGTTGCAAAGCTTGGGTATTGGCTAAGGAGTATGCGCATAAAGAAAAGGTCAATTATGACAAAGTCTCACCAGCAGAGAGATGCGTTTATCATCATGTGTTCAAGGATAGTGTGATTTTACTATCAGGTGACGTGTTAACTACATGTCAGGATGTGTCTAGAATTCCAAAGTTAAAGACACAACTAAGGAGGGAATTCGACATGAAGGGTCTAGAAGCTGCACAACAGATCCTAGAAGTGAAGGTAAGGAGGGACAGAGAAGCAGGAAAATTATGGCCGTTGTCTGAACCCATGTCTACTCCACCAGTAGCAGCACACTTTCAGTTTAGTGTGCAGGGGGGATCATCTACAGTTGAGTTAGATGAGAAGATGCATGTGTCTTATGCAAGAGTAGTGGGATGTCTTATGGCTAGAATCTTCAAAAGACCAGGTTTAACTCAAGGATTGAGCATTGTGTCAAAGTATGTGGTGAATCCGGGTAGACAATACTGGCAAGCAGCGACGTGGATTCTGTATTACTTGAGAAACACAAAGGAGCATGAATTTCTGTTTGAAAGACAACAAGAACAAGCATGCATTGCTGGTCATGAGAGGTCGGATTTGGCAGGAGACTTAGACAAAGGAAGATCTGCAACAAGTAATGTATTTACTTGCAACAAAGGTTCCAAAAGTTGTAGAAAAACTATGGAATCAGCTGAGATATTGTCTACCACTGAAGTAGATTGCATGGCAGTAACAGAAGCTTCCGCAGATGCGTTATGGCTTAATGGGTTAATGAGTGAGTTTGAGATTCAACAAGAAGCTGTGGTGAAGAATGGTGTCATCAATTTGACAAGGAATCAAGTGTTTCAAGCAGAGAGTGAACATGTTGATGTTTGTGGTCATTGCAGCGAAGGCAAGAGGAGTCTTGCCAAGGTGAAGGTTCTTGGAGGTTTTGTTCAGGGTTACTTCAAGAAGATTCAAGATATCCTGAAGGGTGCAACAATCATTTGGTATCAACTCACCAAGGTGGAGATTGTTGTGTTTTGGCTCGTTGATATGATTTGGAAGTTAGGATTCAGTTTAGGAGTCCTAAAACCTTATTGTATCAGCAGCCCCTATTCACTAGAAATAAGAGCTGCAGGGGGTTTCTTAAGGTTAAAGATAGAGAGCACAGAAAACCAAGAGCTAAGGGTGATAGAGAGATCTAGCATCAGCAGCCCCTATTCACTAGAAATAGGAGCTGCAGGGGGTTTCTTAAGGTTAAAGATAGAGAGCACAGAAAACCGAGAGCTAAGGGTGATAGAGAGATCTAGCAGGGAGTTGGGGATTAGCATAGGGATTTCAATAAGTACTTGTAATCAGGTTGTTATTCTCCATAGTGCTAGTGATCTCTCAAGAGAGATCACATCAGTGGACGTAGGCCAGGGTTTTGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC PAH

Protein Analysis

1238

Amino Acids

140.56

Weight (kDa)

8.74

Isoelectric Point (pI)

45.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_2 PF14223 77 - 162 4.2e-16 gag-polypeptide of LTR copia-type
Pol_BBD PF22936 267 - 347 7.5e-28 Pol polyprotein, beta-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000278)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G29785
fragaria_vesca FvH4_1g10046 FvH4_1g17351 FvH4_1g18441 FvH4_1g24222 FvH4_2g00141 FvH4_2g05931 FvH4_2g06671 FvH4_2g14030 FvH4_2g15291 FvH4_2g22611 FvH4_2g22612 FvH4_2g26380 FvH4_2g39202 FvH4_2g39202 FvH4_3g06691 FvH4_3g08580 FvH4_3g20502 FvH4_3g20503 FvH4_3g22421 FvH4_3g42580 FvH4_4g02061 FvH4_4g06794 FvH4_4g08661 FvH4_4g11771 FvH4_4g14601 FvH4_4g14721 FvH4_4g20921 FvH4_4g33601 FvH4_4g33602 FvH4_5g07492 FvH4_5g20321 FvH4_5g21591 FvH4_5g30343 FvH4_5g37875 FvH4_6g04372 FvH4_6g04373 FvH4_6g04373 FvH4_6g21377 FvH4_6g23760 FvH4_6g29471 FvH4_6g43142 FvH4_7g14351 FvH4_7g14352 FvH4_7g22582 FvH4_7g22583 FvH4_7g22584
malus_domestica MD00G1179400.v1.1 MD01G1170500.v1.1 MD03G1286700.v1.1 MD04G1054600.v1.1 MD04G1071700.v1.1 MD04G1193900.v1.1 MD08G1054300.v1.1 MD08G1217500.v1.1 MD09G1034600.v1.1 MD09G1098100.v1.1 MD10G1018300.v1.1 MD10G1220000.v1.1 MD10G1250300.v1.1 MD12G1014400.v1.1 MD15G1021000.v1.1 MD16G1112200.v1.1
prunus_persica Prupe.1G188900_v2.0.a1 Prupe.1G258000_v2.0.a1 Prupe.4G233400_v2.0.a1
pyrus_communis pycom01g10100 pycom02g01340 pycom03g19700 pycom05g01320 pycom07g16000 pycom07g16520 pycom11g26500 pycom13g09380 pycom13g13960 pycom14g08180 pycom14g16810 pycom16g09590
rosa_chinensis RchiOBHm_Chr1g0319891 RchiOBHm_Chr1g0333711 RchiOBHm_Chr2g0115331 RchiOBHm_Chr2g0143411 RchiOBHm_Chr2g0167631 RchiOBHm_Chr3g0448581 RchiOBHm_Chr3g0494991 RchiOBHm_Chr4g0442471 RchiOBHm_Chr6g0262311 RchiOBHm_Chr6g0301831 RchiOBHm_Chr6g0301841 RchiOBHm_Chr7g0183451
rosa_roxburghii Rroxscaffold_1G00039280 Rroxscaffold_1G00047670 Rroxscaffold_1G00066330 Rroxscaffold_2G00107510 Rroxscaffold_3G00255410 Rroxscaffold_4G00301460 Rroxscaffold_5G00352730 Rroxscaffold_7G00166350 Rroxscaffold_7G00166360 Rroxscaffold_7G00203820
rosa_rugosa Rorug03G0240500 Rorug05G0191700 Rorug06G0310200
rosa_samantha Rh2BG287000 Rh4CG073500 Rh4CG176200 Rh4CG191400 Rh5BG356100 Rh5BG434100 Rh6AG110600 Rh6AG421900 Rh6AG422000 Rh6AG422100 Rh6BG428600 Rh6BG428700 Rh6BG428800 Rh6CG435200 Rh6CG435300 Rh6CG435400 Rh6DG422000 Rh6DG422100 Rh6DG422200 Rh7BG190500 Rh7CG172800 Rh7CG225200
rosa_wichuraiana Rw2G044060 Rw6G036540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1909
Acc16I TGCGCA 1 cut(s) 2331
Acc36I ACCTGC 1 cut(s) 1909
AccBSI CCGCTC 1 cut(s) 1963
AccI GTMKAC 5 cut(s) 6, 1169, 2591, 2776, 3002
AciI CCGC 4 cut(s) 88, 876, 1963, 3042
AclWI GGATC 8 cut(s) 303, 907, 920, 934, 1090, 2096, 2518, 2644
AcoI YGGCCR 1 cut(s) 2570
AcsI RAATTY 5 cut(s) 245, 317, 2457, 2489, 2837
AcuI CTGAAG 2 cut(s) 3030, 3287
AdeI CACNNNGTG 1 cut(s) 1638
AfiI CCNNNNNNNGG 3 cut(s) 62, 1041, 2228
AflII CTTAAG 2 cut(s) 3427, 3532
AflIII ACRYGT 6 cut(s) 828, 1122, 1746, 2427, 2438, 3160
AgeI ACCGGT 1 cut(s) 812
AjiI CACGTC 2 cut(s) 2428, 2799
AjnI CCWGG 4 cut(s) 1662, 2095, 2722, 3699
AleI CACNNNNGTG 3 cut(s) 831, 3302, 3686
AloI GAACNNNNNNTCC 2 cut(s) 3216, 3248
Alw21I GWGCWC 3 cut(s) 521, 3449, 3554
Alw26I GTCTC 6 cut(s) 224, 503, 545, 1883, 2365, 2898
AlwI GGATC 8 cut(s) 303, 907, 920, 934, 1090, 2096, 2518, 2644
Ama87I CYCGRG 1 cut(s) 140
AoxI GGCC 6 cut(s) 529, 1032, 1207, 1473, 2570, 3697
ApoI RAATTY 5 cut(s) 245, 317, 2457, 2489, 2837
ArsI GACNNNNNNTTYG 1 cut(s) 38
AsiGI ACCGGT 1 cut(s) 812
Asp700I GAANNNNTTC 3 cut(s) 44, 480, 1604
AspA2I CCTAGG 1 cut(s) 916
AspLEI GCGC 3 cut(s) 1218, 1400, 2332
AspS9I GGNCC 2 cut(s) 179, 1473
AsuC2I CCSGG 2 cut(s) 890, 2772
AvaI CYCGRG 1 cut(s) 140
AvaII GGWCC 1 cut(s) 179
AvrII CCTAGG 1 cut(s) 916
BamHI GGATCC 1 cut(s) 912
BanII GRGCYC 1 cut(s) 521
BbsI GAAGAC 4 cut(s) 266, 1069, 1826, 1832
Bbv12I GWGCWC 3 cut(s) 521, 3449, 3554
BccI CCATC 4 cut(s) 274, 679, 1807, 2146
BceAI ACGGC 3 cut(s) 717, 1229, 2557
BciT130I CCWGG 4 cut(s) 1664, 2097, 2724, 3701
BciVI GTATCC 1 cut(s) 802
BcnI CCSGG 2 cut(s) 890, 2772
BcoDI GTCTC 6 cut(s) 224, 503, 545, 1883, 2365, 2898
BfmI CTRYAG 7 cut(s) 268, 299, 648, 2124, 2643, 3414, 3519
BfrI CTTAAG 2 cut(s) 3427, 3532
BfuAI ACCTGC 1 cut(s) 1909
BfuI GTATCC 1 cut(s) 802
BglII AGATCT 4 cut(s) 121, 2921, 3479, 3584
BlnI CCTAGG 1 cut(s) 916
BmcAI AGTACT 1 cut(s) 3628
Bme1390I CCNGG 6 cut(s) 890, 1664, 2097, 2724, 2772, 3701
Bme18I GGWCC 1 cut(s) 179
BmeT110I CYCGRG 1 cut(s) 140
BmgBI CACGTC 2 cut(s) 2428, 2799
BmgT120I GGNCC 2 cut(s) 179, 1473
BmiI GGNNCC 4 cut(s) 180, 914, 2214, 2959
BmrFI CCNGG 6 cut(s) 890, 1664, 2097, 2724, 2772, 3701
BmsI GCATC 6 cut(s) 634, 1650, 2366, 2655, 3037, 3495
BpiI GAAGAC 4 cut(s) 266, 1069, 1826, 1832
BpmI CTGGAG 3 cut(s) 989, 1646, 1878
Bpu10I CCTNAGC 4 cut(s) 1899, 2319, 3464, 3569
BpuEI CTTGAG 6 cut(s) 440, 507, 2060, 2718, 2836, 3655
BpuMI CCSGG 2 cut(s) 890, 2772
BsaAI YACGTR 1 cut(s) 2112
BsaI GGTCTC 1 cut(s) 503
BsaJI CCNNGG 6 cut(s) 889, 916, 1497, 3207, 3300, 3700
BsaWI WCCGGW 2 cut(s) 440, 812
BsaXI ACNNNNNCTCC 4 cut(s) 700, 730, 3216, 3246
Bsc4I CCNNNNNNNGG 3 cut(s) 62, 1041, 2228
Bse118I RCCGGY 2 cut(s) 812, 1034
Bse1I ACTGG 3 cut(s) 567, 2600, 2789
Bse3DI GCAATG 5 cut(s) 1170, 1202, 1582, 2871, 3178
BseBI CCWGG 4 cut(s) 1664, 2097, 2724, 3701
BseDI CCNNGG 6 cut(s) 889, 916, 1497, 3207, 3300, 3700
BseGI GGATG 9 cut(s) 76, 102, 285, 690, 1630, 1692, 2157, 2452, 2699
BseLI CCNNNNNNNGG 3 cut(s) 62, 1041, 2228
BseMI GCAATG 5 cut(s) 1170, 1202, 1582, 2871, 3178
BseMII CTCAG 6 cut(s) 1164, 1182, 1913, 2003, 2037, 2982
BseNI ACTGG 3 cut(s) 567, 2600, 2789
BseRI GAGGAG 3 cut(s) 106, 878, 3211
BseYI CCCAGC 1 cut(s) 1733
BsgI GTGCAG 2 cut(s) 2499, 2648
BshFI GGCC 6 cut(s) 531, 1034, 1209, 1475, 2572, 3699
BshTI ACCGGT 1 cut(s) 812
BsiHKAI GWGCWC 3 cut(s) 521, 3449, 3554
BsiHKCI CYCGRG 1 cut(s) 140
BsiSI CCGG 5 cut(s) 441, 813, 889, 1035, 2771
BslFI GGGAC 2 cut(s) 1153, 2561
BslI CCNNNNNNNGG 3 cut(s) 62, 1041, 2228
BsmAI GTCTC 6 cut(s) 224, 503, 545, 1883, 2365, 2898
BsmFI GGGAC 2 cut(s) 1153, 2561
BsmI GAATGC 3 cut(s) 948, 1239, 1328
BsnI GGCC 6 cut(s) 531, 1034, 1209, 1475, 2572, 3699
Bso31I GGTCTC 1 cut(s) 503
BsoBI CYCGRG 1 cut(s) 140
Bsp1286I GDGCHC 3 cut(s) 521, 3449, 3554
Bsp1407I TGTACA 2 cut(s) 818, 1125
BspACI CCGC 4 cut(s) 88, 876, 1963, 3042
BspANI GGCC 6 cut(s) 531, 1034, 1209, 1475, 2572, 3699
BspCNI CTCAG 6 cut(s) 1165, 1183, 1912, 2002, 2036, 2983
BspHI TCATGA 1 cut(s) 2881
BspLI GGNNCC 4 cut(s) 180, 914, 2214, 2959
BspMAI CTGCAG 4 cut(s) 303, 652, 3418, 3523
BspMI ACCTGC 1 cut(s) 1909
BspPI GGATC 8 cut(s) 303, 907, 920, 934, 1090, 2096, 2518, 2644
BspTI CTTAAG 2 cut(s) 3427, 3532
BspTNI GGTCTC 1 cut(s) 503
BsrBI CCGCTC 1 cut(s) 1963
BsrDI GCAATG 5 cut(s) 1170, 1202, 1582, 2871, 3178
BsrFI RCCGGY 2 cut(s) 812, 1034
BsrGI TGTACA 2 cut(s) 818, 1125
BsrI ACTGG 3 cut(s) 567, 2600, 2789
BssAI RCCGGY 2 cut(s) 812, 1034
BssECI CCNNGG 6 cut(s) 889, 916, 1497, 3207, 3300, 3700
BssT1I CCWWGG 3 cut(s) 916, 3207, 3300
Bst2UI CCWGG 4 cut(s) 1664, 2097, 2724, 3701
Bst4CI ACNGT 5 cut(s) 906, 1614, 1648, 1974, 2647
Bst6I CTCTTC 4 cut(s) 95, 363, 1511, 1881
BstAFI CTTAAG 2 cut(s) 3427, 3532
BstAPI GCANNNNNTGC 1 cut(s) 2873
BstAUI TGTACA 2 cut(s) 818, 1125
BstBAI YACGTR 1 cut(s) 2112
BstC8I GCNNGC 4 cut(s) 1721, 1731, 2790, 2869
BstEII GGTNACC 2 cut(s) 1737, 1794
BstF5I GGATG 9 cut(s) 76, 102, 285, 690, 1630, 1692, 2157, 2452, 2699
BstHHI GCGC 3 cut(s) 1218, 1400, 2332
BstMAI GTCTC 6 cut(s) 224, 503, 545, 1883, 2365, 2898
BstMWI GCNNNNNNNGC 8 cut(s) 1201, 1215, 1581, 1915, 1924, 2873, 3189, 3492
BstNI CCWGG 4 cut(s) 1664, 2097, 2724, 3701
BstNSI RCATGY 7 cut(s) 832, 1723, 1733, 2442, 2672, 2871, 3164
BstPI GGTNACC 2 cut(s) 1737, 1794
BstSCI CCNGG 6 cut(s) 888, 1662, 2095, 2722, 2770, 3699
BstSFI CTRYAG 7 cut(s) 268, 299, 648, 2124, 2643, 3414, 3519
BstV2I GAAGAC 4 cut(s) 266, 1069, 1826, 1832
BstX2I RGATCY 8 cut(s) 121, 295, 912, 2101, 2523, 2921, 3479, 3584
BstXI CCANNNNNNTGG 1 cut(s) 3707
BstYI RGATCY 8 cut(s) 121, 295, 912, 2101, 2523, 2921, 3479, 3584
BsuI GTATCC 1 cut(s) 802
BsuRI GGCC 6 cut(s) 531, 1034, 1209, 1475, 2572, 3699
BtrI CACGTC 2 cut(s) 2428, 2799
BtsCI GGATG 9 cut(s) 76, 102, 285, 690, 1630, 1692, 2157, 2452, 2699
BtsI GCAGTG 3 cut(s) 1591, 1724, 2006
BtsIMutI CAGTG 7 cut(s) 911, 936, 1591, 1724, 2006, 3006, 3693
BveI ACCTGC 1 cut(s) 1909
Cac8I GCNNGC 4 cut(s) 1721, 1731, 2790, 2869
CciI TCATGA 1 cut(s) 2881
CfoI GCGC 3 cut(s) 1218, 1400, 2332
Cfr10I RCCGGY 2 cut(s) 812, 1034
Cfr13I GGNCC 2 cut(s) 179, 1473
CseI GACGC 1 cut(s) 245
CsiI ACCWGGT 1 cut(s) 2722
CspAI ACCGGT 1 cut(s) 812
CspCI CAANNNNNGTGG 4 cut(s) 2670, 2705, 2742, 2777
DraIII CACNNNGTG 1 cut(s) 1638
EaeI YGGCCR 1 cut(s) 2570
Eam1104I CTCTTC 4 cut(s) 95, 363, 1511, 1881
EarI CTCTTC 4 cut(s) 95, 363, 1511, 1881
EciI GGCGGA 1 cut(s) 103
Ecl136II GAGCTC 1 cut(s) 519
Eco130I CCWWGG 3 cut(s) 916, 3207, 3300
Eco147I AGGCCT 2 cut(s) 531, 1209
Eco24I GRGCYC 1 cut(s) 521
Eco31I GGTCTC 1 cut(s) 503
Eco32I GATATC 2 cut(s) 1011, 3262
Eco47I GGWCC 1 cut(s) 179
Eco53kI GAGCTC 1 cut(s) 519
Eco57I CTGAAG 2 cut(s) 3030, 3287
Eco88I CYCGRG 1 cut(s) 140
Eco91I GGTNACC 2 cut(s) 1737, 1794
EcoICRI GAGCTC 1 cut(s) 519
EcoO109I RGGNCCY 1 cut(s) 179
EcoO65I GGTNACC 2 cut(s) 1737, 1794
EcoRI GAATTC 2 cut(s) 2457, 2489
EcoRII CCWGG 4 cut(s) 1662, 2095, 2722, 3699
EcoRV GATATC 2 cut(s) 1011, 3262
EcoT14I CCWWGG 3 cut(s) 916, 3207, 3300
EcoT22I ATGCAT 4 cut(s) 948, 2280, 2670, 2873
EcoT38I GRGCYC 1 cut(s) 521
ErhI CCWWGG 3 cut(s) 916, 3207, 3300
FalI AAGNNNNNCTT 4 cut(s) 59, 91, 516, 548
FaqI GGGAC 2 cut(s) 1153, 2561
FblI GTMKAC 5 cut(s) 6, 1169, 2591, 2776, 3002
FokI GGATG 9 cut(s) 83, 109, 292, 697, 1637, 1699, 2164, 2459, 2706
FriOI GRGCYC 1 cut(s) 521
FspAI RTGCGCAY 1 cut(s) 2331
FspI TGCGCA 1 cut(s) 2331
GlaI GCGC 3 cut(s) 1217, 1399, 2331
GsaI CCCAGC 1 cut(s) 1737
GsuI CTGGAG 3 cut(s) 989, 1646, 1878
HaeIII GGCC 6 cut(s) 531, 1034, 1209, 1475, 2572, 3699
HapII CCGG 5 cut(s) 441, 813, 889, 1035, 2771
HgaI GACGC 1 cut(s) 245
HhaI GCGC 3 cut(s) 1218, 1400, 2332
Hin6I GCGC 3 cut(s) 1216, 1398, 2330
HinP1I GCGC 3 cut(s) 1216, 1398, 2330
HincII GTYRAC 3 cut(s) 564, 1768, 2433
HindII GTYRAC 3 cut(s) 564, 1768, 2433
HindIII AAGCTT 3 cut(s) 1130, 2304, 3036
HpaI GTTAAC 2 cut(s) 1768, 2433
HpaII CCGG 5 cut(s) 441, 813, 889, 1035, 2771
Hpy99I CGWCG 1 cut(s) 2800
HpyCH4III ACNGT 5 cut(s) 906, 1614, 1648, 1974, 2647
HpyCH4IV ACGT 7 cut(s) 773, 1122, 1748, 2111, 2427, 2798, 3693
HpyF10VI GCNNNNNNNGC 8 cut(s) 1201, 1215, 1581, 1915, 1924, 2873, 3189, 3492
HpySE526I ACGT 7 cut(s) 773, 1122, 1748, 2111, 2427, 2798, 3693
HspAI GCGC 3 cut(s) 1216, 1398, 2330
KspAI GTTAAC 2 cut(s) 1768, 2433
LmnI GCTCC 8 cut(s) 785, 1395, 1906, 1924, 1996, 2156, 2830, 3515
LweI GCATC 6 cut(s) 634, 1650, 2366, 2655, 3037, 3495
MabI ACCWGGT 1 cut(s) 2722
MaeII ACGT 7 cut(s) 773, 1122, 1748, 2111, 2427, 2798, 3693
MbiI CCGCTC 1 cut(s) 1963
MflI RGATCY 8 cut(s) 121, 295, 912, 2101, 2523, 2921, 3479, 3584
MhlI GDGCHC 3 cut(s) 521, 3449, 3554
MlyI GAGTC 7 cut(s) 893, 1231, 1885, 2015, 2216, 3208, 3372
MmeI TCCRAC 5 cut(s) 807, 2129, 2129, 2145, 2871
Mph1103I ATGCAT 4 cut(s) 948, 2280, 2670, 2873
MroXI GAANNNNTTC 3 cut(s) 44, 480, 1604
MslI CAYNNNNRTG 5 cut(s) 831, 1646, 3165, 3302, 3686
MspA1I CMGCKG 2 cut(s) 876, 2990
MspCI CTTAAG 2 cut(s) 3427, 3532
MspI CCGG 5 cut(s) 441, 813, 889, 1035, 2771
MspR9I CCNGG 6 cut(s) 890, 1664, 2097, 2724, 2772, 3701
Mva1269I GAATGC 3 cut(s) 948, 1239, 1328
MvaI CCWGG 4 cut(s) 1664, 2097, 2724, 3701
MwoI GCNNNNNNNGC 8 cut(s) 1201, 1215, 1581, 1915, 1924, 2873, 3189, 3492
NciI CCSGG 2 cut(s) 890, 2772
NlaIV GGNNCC 4 cut(s) 180, 914, 2214, 2959
NmuCI GTSAC 4 cut(s) 697, 1794, 1974, 2423
NsbI TGCGCA 1 cut(s) 2331
NsiI ATGCAT 4 cut(s) 948, 2280, 2670, 2873
NspI RCATGY 7 cut(s) 832, 1723, 1733, 2442, 2672, 2871, 3164
OliI CACNNNNGTG 3 cut(s) 831, 3302, 3686
PaeI GCATGC 3 cut(s) 1723, 1733, 2871
PagI TCATGA 1 cut(s) 2881
PaqCI CACCTGC 1 cut(s) 1909
PceI AGGCCT 2 cut(s) 531, 1209
PciI ACATGT 3 cut(s) 828, 2438, 3160
PctI GAATGC 3 cut(s) 948, 1239, 1328
PdmI GAANNNNTTC 3 cut(s) 44, 480, 1604
PflFI GACNNNGTC 3 cut(s) 20, 221, 2357
PfoI TCCNGGA 2 cut(s) 1662, 2095
PinAI ACCGGT 1 cut(s) 812
PleI GAGTC 7 cut(s) 893, 1230, 1885, 2015, 2215, 3207, 3371
PpsI GAGTC 7 cut(s) 893, 1230, 1885, 2015, 2215, 3207, 3371
Ppu21I YACGTR 1 cut(s) 2112
PpuMI RGGWCCY 1 cut(s) 179
PscI ACATGT 3 cut(s) 828, 2438, 3160
Psp124BI GAGCTC 1 cut(s) 521
Psp5II RGGWCCY 1 cut(s) 179
Psp6I CCWGG 4 cut(s) 1662, 2095, 2722, 3699
PspEI GGTNACC 2 cut(s) 1737, 1794
PspFI CCCAGC 1 cut(s) 1733
PspGI CCWGG 4 cut(s) 1662, 2095, 2722, 3699
PspN4I GGNNCC 4 cut(s) 180, 914, 2214, 2959
PspPI GGNCC 2 cut(s) 179, 1473
PspPPI RGGWCCY 1 cut(s) 179
PstI CTGCAG 4 cut(s) 303, 652, 3418, 3523
PsuI RGATCY 8 cut(s) 121, 295, 912, 2101, 2523, 2921, 3479, 3584
PsyI GACNNNGTC 3 cut(s) 20, 221, 2357
PvuII CAGCTG 1 cut(s) 2990
RseI CAYNNNNRTG 5 cut(s) 831, 1646, 3165, 3302, 3686
SacI GAGCTC 1 cut(s) 521
Sau96I GGNCC 2 cut(s) 179, 1473
ScaI AGTACT 1 cut(s) 3628
SchI GAGTC 7 cut(s) 893, 1231, 1885, 2015, 2216, 3208, 3372
ScrFI CCNGG 6 cut(s) 890, 1664, 2097, 2724, 2772, 3701
SduI GDGCHC 3 cut(s) 521, 3449, 3554
SexAI ACCWGGT 1 cut(s) 2722
SfaNI GCATC 6 cut(s) 634, 1650, 2366, 2655, 3037, 3495
SfcI CTRYAG 7 cut(s) 268, 299, 648, 2124, 2643, 3414, 3519
SinI GGWCC 1 cut(s) 179
SmiMI CAYNNNNRTG 5 cut(s) 831, 1646, 3165, 3302, 3686
SmlI CTYRAG 8 cut(s) 455, 522, 2039, 2733, 2815, 3427, 3532, 3670
SmoI CTYRAG 8 cut(s) 455, 522, 2039, 2733, 2815, 3427, 3532, 3670
SphI GCATGC 3 cut(s) 1723, 1733, 2871
SseBI AGGCCT 2 cut(s) 531, 1209
SsiI CCGC 4 cut(s) 88, 876, 1963, 3042
SspI AATATT 1 cut(s) 1565
SstI GAGCTC 1 cut(s) 521
StuI AGGCCT 2 cut(s) 531, 1209
StyD4I CCNGG 6 cut(s) 888, 1662, 2095, 2722, 2770, 3699
StyI CCWWGG 3 cut(s) 916, 3207, 3300
TaaI ACNGT 5 cut(s) 906, 1614, 1648, 1974, 2647
TaiI ACGT 7 cut(s) 776, 1125, 1751, 2114, 2430, 2801, 3696
TaqI TCGA 3 cut(s) 24, 1944, 2493
TaqII GACCGA 2 cut(s) 527, 1782
TatI WGTACW 5 cut(s) 618, 716, 818, 1125, 3626
TauI GCSGC 1 cut(s) 1966
TscAI CASTG 7 cut(s) 911, 936, 1591, 1731, 2013, 3013, 3693
TseFI GTSAC 4 cut(s) 697, 1794, 1974, 2423
Tsp45I GTSAC 4 cut(s) 697, 1794, 1974, 2423
TspRI CASTG 7 cut(s) 911, 936, 1591, 1731, 2013, 3013, 3693
Tth111I GACNNNGTC 3 cut(s) 20, 221, 2357
Vha464I CTTAAG 2 cut(s) 3427, 3532
VpaK11BI GGWCC 1 cut(s) 179
XapI RAATTY 5 cut(s) 245, 317, 2457, 2489, 2837
XbaI TCTAGA 4 cut(s) 1447, 1543, 2453, 2506
XceI RCATGY 7 cut(s) 832, 1723, 1733, 2442, 2672, 2871, 3164
XcmI CCANNNNNNNNNTGG 2 cut(s) 1749, 1879
XmaJI CCTAGG 1 cut(s) 916
XmiI GTMKAC 5 cut(s) 6, 1169, 2591, 2776, 3002
XmnI GAANNNNTTC 3 cut(s) 44, 480, 1604
ZrmI AGTACT 1 cut(s) 3628
Zsp2I ATGCAT 4 cut(s) 948, 2280, 2670, 2873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.