MD04G1071700.v1.1

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000278
Physical Location & Seq
Reverse (-)
9701658 .. 9705977
4320 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1071700.v1.1.491

Sequence Viewer

Length: 2142 bp
ATGGCACCTTATGCTTACTTGGCAATGTACAAAGTCTGTTATGGGCGTGGTTGTACGTACGGCGACATTTTAGCTGGTCTTGATGCTGCTGTTGAAGATGGCTTGGACGTGCTTTCCCTTTCACTTGGAGGCCCCTCACTTCCTTTCTACAATGATGTGATTGCAATCGGTGCATTTGCAGCAATTAAAAAAGGAATATTTTTCAGCTGTGCGGCTGGAAATAACGGAGCAGGAAATCACCATAAATCTTTATCAAATGAGGCCCCATGGATTCTCACAGTTGGAGCAAGTACCACTGATAGAATACTTAAATCGGAAGATGAGAAGCTCAACACCTATGGCAGTATAATCAGAGATGCACTTGCTCCCAAGGTCGCTCACTTTTCTTCACGAGGACCAAGCAGATCAAGCCCTGGAATTTTGAAACCTGACATCACTGGACCCGGTGTTGACATCCTAGCAGCATGGCCTGAATCAGTGGACAATGCCACACCTCCTAATCCTAAGGCAACATTTAACATAATTTCAGGTACCTCAATGGCAACTCCTCACCTAAGCGGCATTGCAGCTTTGATCAAGAAATCACACCCTGACTGGTCTCCAGCTGCTATTAAATCTGCCATCATGACAACCGCTGATGTACTAAACCTCGCAGGCAAGCCCATCATTGACCAAGGGCTTTTCCCCGTGGACGTCTTTGCAATTGGTGCTTGTTGGATTTTCCTGTGGAGGGTTAAGATGCGAGCTGTGCTAGTTCAACAAGGGTTGCTTAAGGCGCTACAGGGTGTGGAAGCCTTGCCACAAGATTGGTCTGCTGAAGAAAAAGAAGACGCTTTGAATCGAGCACATAGTGCAATTATGTTATCACTATCTGATGAAGTTCTTCGTGAGATGGAAAATGAAACTACTGCAGCTGGTTTGTGGCTTAAGTTGGAGAGTATATACATGACCAAATCCCTCACCAATCGTTTGTATTTGAAGCAACGTTTGTACACTCTCCGTATGACTGAAGGTACACCTATTCAAAACCATCTTGATGAGTTTAATAAGGTTATTATGGATTTGAAAAGTATGGATAATAAAATTAATGATGAGGATCAATCTTTGATTTTGTTGTGTTCTTTACCTCCTTCGTATGCGAATTTTGTTGAAACTTTGTTATATGGGAGAGATTCTATTTGTATGGAGAATGTAAAAGCTGCTTTGAATTCAAGAGAGTTAAAGAACAAAGTATTTGGAAATTTGAATGATTCTCATTCTGAGGCTTTGATAGCAAGAGGCAGAGATAGGGAATATGGTTCAGGGAGTAATAAAGGAAATTTGAGATCGAAGTCTAAGACCAGAAACAACACATGCAACTATTGTCGCAAGGAAGGGCATTGGAAAGTTGATTGCCCAAAACTGAAAGATAATGCTGGTGCGAGTGCGAATGTCTTGCTCTTCATCGGTGTTTTTATTGTTTTGGATATTGGGCATATTTTTTGTATGTGGTGGAGTTCGTTGTGTGTCCAATTTTGGTATGGTGTGCTTGATCTTTTGTTGTTCGTATTTTGGGTGCTTATGATGATTGTTGAGTCAACTCTGAGTTGCTTTCGGGAATTTCGCCAAGTGATCACTGAACCAAAGATAATTGTACTACATTTTGTGGCTATTGGGAGAGAAGGAATTTGGTATATGTTTCCATCTTCTCCATTTGTTCTTTGTGAGAGTGAGAGCTATTGGGTGTATTGGAATTTCCTCGCCGTCTCGGAACTGGACGTAGGCTTACGCCGAACCAGTATAAATTATTGTGTTCTTTGGATTATTTGTCTTGTCATATTTTACCGTATTAGCTTATTGGTTTCATATTTGACGCTTCCGCACAACAGTGCTGGCCATGTTAACCCTGCAAAGGCAAATGACCCAGGGCTCATCTTCGACATAAAACCAGAGGATTACTTTCCTTACTTGTGCGGTTTGAATTACAATGAAACAGCGATTAAGATCATCACCCAACAAACTGTTAAATGCTCGGAAGTTGGAGTAATGCCAGAAGCACAGCTCAATTACCCTTCATTTTCTATTAAAGCAGGTCCCAATCAGACTCAGTCCCAGTACTATACAAGGGCTGTGAAGAACGTTGGCCCGGCTACTTCAACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

714

Amino Acids

79.03

Weight (kDa)

6.68

Isoelectric Point (pI)

35.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 2 - 213 8.4e-33 Subtilase family
Retrotran_gag_2 PF14223 247 - 406 3e-36 gag-polypeptide of LTR copia-type
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000278)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G29785
fragaria_vesca FvH4_1g10046 FvH4_1g17351 FvH4_1g18441 FvH4_1g24222 FvH4_2g00141 FvH4_2g05931 FvH4_2g06671 FvH4_2g14030 FvH4_2g15291 FvH4_2g22611 FvH4_2g22612 FvH4_2g26380 FvH4_2g39202 FvH4_2g39202 FvH4_3g06691 FvH4_3g08580 FvH4_3g20502 FvH4_3g20503 FvH4_3g22421 FvH4_3g42580 FvH4_4g02061 FvH4_4g06794 FvH4_4g08661 FvH4_4g11771 FvH4_4g14601 FvH4_4g14721 FvH4_4g20921 FvH4_4g33601 FvH4_4g33602 FvH4_5g07492 FvH4_5g20321 FvH4_5g21591 FvH4_5g30343 FvH4_5g37875 FvH4_6g04372 FvH4_6g04373 FvH4_6g04373 FvH4_6g21377 FvH4_6g23760 FvH4_6g29471 FvH4_6g43142 FvH4_7g14351 FvH4_7g14352 FvH4_7g22582 FvH4_7g22583 FvH4_7g22584
malus_domestica MD00G1179400.v1.1 MD01G1170500.v1.1 MD03G1286700.v1.1 MD04G1054600.v1.1 MD04G1071700.v1.1 MD04G1193900.v1.1 MD08G1054300.v1.1 MD08G1217500.v1.1 MD09G1034600.v1.1 MD09G1098100.v1.1 MD10G1018300.v1.1 MD10G1220000.v1.1 MD10G1250300.v1.1 MD12G1014400.v1.1 MD15G1021000.v1.1 MD16G1112200.v1.1
prunus_persica Prupe.1G188900_v2.0.a1 Prupe.1G258000_v2.0.a1 Prupe.4G233400_v2.0.a1
pyrus_communis pycom01g10100 pycom02g01340 pycom03g19700 pycom05g01320 pycom07g16000 pycom07g16520 pycom11g26500 pycom13g09380 pycom13g13960 pycom14g08180 pycom14g16810 pycom16g09590
rosa_chinensis RchiOBHm_Chr1g0319891 RchiOBHm_Chr1g0333711 RchiOBHm_Chr2g0115331 RchiOBHm_Chr2g0143411 RchiOBHm_Chr2g0167631 RchiOBHm_Chr3g0448581 RchiOBHm_Chr3g0494991 RchiOBHm_Chr4g0442471 RchiOBHm_Chr6g0262311 RchiOBHm_Chr6g0301831 RchiOBHm_Chr6g0301841 RchiOBHm_Chr7g0183451
rosa_roxburghii Rroxscaffold_1G00039280 Rroxscaffold_1G00047670 Rroxscaffold_1G00066330 Rroxscaffold_2G00107510 Rroxscaffold_3G00255410 Rroxscaffold_4G00301460 Rroxscaffold_5G00352730 Rroxscaffold_7G00166350 Rroxscaffold_7G00166360 Rroxscaffold_7G00203820
rosa_rugosa Rorug03G0240500 Rorug05G0191700 Rorug06G0310200
rosa_samantha Rh2BG287000 Rh4CG073500 Rh4CG176200 Rh4CG191400 Rh5BG356100 Rh5BG434100 Rh6AG110600 Rh6AG421900 Rh6AG422000 Rh6AG422100 Rh6BG428600 Rh6BG428700 Rh6BG428800 Rh6CG435200 Rh6CG435300 Rh6CG435400 Rh6DG422000 Rh6DG422100 Rh6DG422200 Rh7BG190500 Rh7CG172800 Rh7CG225200
rosa_wichuraiana Rw2G044060 Rw6G036540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 696
Acc36I ACCTGC 1 cut(s) 2060
Acc65I GGTACC 1 cut(s) 530
AccB1I GGYRCC 2 cut(s) 4, 530
AciI CCGC 5 cut(s) 212, 558, 633, 1859, 1953
AclI AACGTT 2 cut(s) 985, 2118
AclWI GGATC 1 cut(s) 1104
AcoI YGGCCR 1 cut(s) 1873
AcsI RAATTY 8 cut(s) 417, 1141, 1207, 1240, 1318, 1598, 1665, 1732
AcuI CTGAAG 2 cut(s) 837, 1029
AcyI GRCGYC 1 cut(s) 693
AdeI CACNNNGTG 1 cut(s) 851
AfiI CCNNNNNNNGG 2 cut(s) 730, 1891
AflII CTTAAG 2 cut(s) 770, 926
AjiI CACGTC 1 cut(s) 109
AjnI CCWGG 2 cut(s) 412, 1903
AleI CACNNNNGTG 1 cut(s) 1866
Alw21I GWGCWC 1 cut(s) 847
Alw26I GTCTC 2 cut(s) 603, 1750
AlwI GGATC 1 cut(s) 1104
AoxI GGCC 5 cut(s) 130, 261, 467, 1873, 2122
ApeKI GCWGC 7 cut(s) 86, 179, 461, 566, 605, 911, 1199
ApoI RAATTY 8 cut(s) 417, 1141, 1207, 1240, 1318, 1598, 1665, 1732
AseI ATTAAT 1 cut(s) 1086
Asp700I GAANNNNTTC 1 cut(s) 882
Asp718I GGTACC 1 cut(s) 530
AspLEI GCGC 1 cut(s) 778
AspS9I GGNCC 6 cut(s) 131, 262, 395, 440, 2072, 2123
AsuC2I CCSGG 2 cut(s) 444, 2126
AsuHPI GGTGA 4 cut(s) 230, 542, 952, 1981
AvaII GGWCC 3 cut(s) 395, 440, 2072
AxyI CCTNAGG 1 cut(s) 504
BalI TGGCCA 1 cut(s) 1875
BanI GGYRCC 2 cut(s) 4, 530
BanII GRGCYC 1 cut(s) 1911
BauI CACGAG 1 cut(s) 390
BbsI GAAGAC 1 cut(s) 834
Bbv12I GWGCWC 1 cut(s) 847
BbvI GCAGC 7 cut(s) 73, 191, 473, 578, 592, 923, 1186
BccI CCATC 6 cut(s) 92, 629, 671, 886, 1038, 1690
BceAI ACGGC 2 cut(s) 76, 1727
BcgI CGANNNNNNTGC 2 cut(s) 1417, 1451
BciT130I CCWGG 2 cut(s) 414, 1905
BclI TGATCA 2 cut(s) 573, 1611
BcnI CCSGG 2 cut(s) 444, 2126
BcoDI GTCTC 2 cut(s) 603, 1750
BfaI CTAG 2 cut(s) 458, 752
BfmI CTRYAG 2 cut(s) 779, 909
BfoI RGCGCY 1 cut(s) 779
BfrI CTTAAG 2 cut(s) 770, 926
BfuAI ACCTGC 1 cut(s) 2060
BisI GCNGC 9 cut(s) 87, 180, 213, 462, 559, 567, 606, 912, 1200
BlsI GCNGC 9 cut(s) 88, 181, 214, 463, 560, 568, 607, 913, 1201
BmcAI AGTACT 1 cut(s) 2096
Bme1390I CCNGG 4 cut(s) 414, 444, 1905, 2126
Bme18I GGWCC 3 cut(s) 395, 440, 2072
BmgBI CACGTC 1 cut(s) 109
BmgT120I GGNCC 6 cut(s) 131, 262, 395, 440, 2072, 2123
BmiI GGNNCC 6 cut(s) 6, 133, 264, 442, 532, 2074
BmrFI CCNGG 4 cut(s) 414, 444, 1905, 2126
BmrI ACTGGG 1 cut(s) 2086
BmsI GCATC 3 cut(s) 73, 346, 729
BmuI ACTGGG 1 cut(s) 2086
BpiI GAAGAC 1 cut(s) 834
BpmI CTGGAG 1 cut(s) 585
Bpu10I CCTNAGC 1 cut(s) 554
BpuMI CCSGG 2 cut(s) 444, 2126
BsaAI YACGTR 1 cut(s) 57
BsaBI GATNNNNATC 3 cut(s) 164, 1095, 1982
BsaHI GRCGYC 1 cut(s) 693
BsaI GGTCTC 1 cut(s) 603
BsaJI CCNNGG 7 cut(s) 266, 369, 412, 673, 687, 1903, 1904
Bsc4I CCNNNNNNNGG 2 cut(s) 730, 1891
Bse1I ACTGG 5 cut(s) 442, 599, 1758, 1776, 2092
Bse21I CCTNAGG 1 cut(s) 504
Bse3DI GCAATG 2 cut(s) 30, 561
Bse8I GATNNNNATC 3 cut(s) 164, 1095, 1982
BseBI CCWGG 2 cut(s) 414, 1905
BseDI CCNNGG 7 cut(s) 266, 369, 412, 673, 687, 1903, 1904
BseGI GGATG 1 cut(s) 453
BseJI GATNNNNATC 3 cut(s) 164, 1095, 1982
BseLI CCNNNNNNNGG 2 cut(s) 730, 1891
BseMI GCAATG 2 cut(s) 30, 561
BseMII CTCAG 3 cut(s) 1251, 1574, 2099
BseNI ACTGG 5 cut(s) 442, 599, 1758, 1776, 2092
BseRI GAGGAG 1 cut(s) 537
BseXI GCAGC 7 cut(s) 73, 191, 473, 578, 592, 923, 1186
BshFI GGCC 5 cut(s) 132, 263, 469, 1875, 2124
BshNI GGYRCC 2 cut(s) 4, 530
BsiHKAI GWGCWC 1 cut(s) 847
BsiSI CCGG 2 cut(s) 444, 2126
BsiWI CGTACG 1 cut(s) 57
BslFI GGGAC 2 cut(s) 2058, 2074
BslI CCNNNNNNNGG 2 cut(s) 730, 1891
BsmAI GTCTC 2 cut(s) 603, 1750
BsmBI CGTCTC 1 cut(s) 1750
BsmFI GGGAC 2 cut(s) 2058, 2074
BsnI GGCC 5 cut(s) 132, 263, 469, 1875, 2124
Bso31I GGTCTC 1 cut(s) 603
Bsp1286I GDGCHC 2 cut(s) 847, 1911
Bsp1407I TGTACA 2 cut(s) 27, 990
Bsp143I GATC 7 cut(s) 404, 573, 1096, 1325, 1531, 1611, 1983
Bsp19I CCATGG 1 cut(s) 266
BspACI CCGC 5 cut(s) 212, 558, 633, 1859, 1953
BspANI GGCC 5 cut(s) 132, 263, 469, 1875, 2124
BspCNI CTCAG 3 cut(s) 1252, 1575, 2098
BspHI TCATGA 1 cut(s) 624
BspLI GGNNCC 6 cut(s) 6, 133, 264, 442, 532, 2074
BspMAI CTGCAG 1 cut(s) 913
BspMI ACCTGC 1 cut(s) 2060
BspPI GGATC 1 cut(s) 1104
BspQI GCTCTTC 1 cut(s) 1445
BspT107I GGYRCC 2 cut(s) 4, 530
BspTI CTTAAG 2 cut(s) 770, 926
BspTNI GGTCTC 1 cut(s) 603
BsrDI GCAATG 2 cut(s) 30, 561
BsrGI TGTACA 2 cut(s) 27, 990
BsrI ACTGG 5 cut(s) 442, 599, 1758, 1776, 2092
BssECI CCNNGG 7 cut(s) 266, 369, 412, 673, 687, 1903, 1904
BssMI GATC 7 cut(s) 404, 573, 1096, 1325, 1531, 1611, 1983
BssNI GRCGYC 1 cut(s) 693
BssSI CACGAG 1 cut(s) 390
BssT1I CCWWGG 3 cut(s) 266, 369, 673
Bst2BI CACGAG 1 cut(s) 390
Bst2UI CCWGG 2 cut(s) 414, 1905
Bst4CI ACNGT 4 cut(s) 280, 1826, 1868, 2002
Bst6I CTCTTC 1 cut(s) 1445
BstACI GRCGYC 1 cut(s) 693
BstAFI CTTAAG 2 cut(s) 770, 926
BstAPI GCANNNNNTGC 4 cut(s) 11, 170, 707, 851
BstAUI TGTACA 2 cut(s) 27, 990
BstBAI YACGTR 1 cut(s) 57
BstC8I GCNNGC 4 cut(s) 655, 659, 744, 1873
BstDEI CTNAG 6 cut(s) 504, 554, 1260, 1335, 1583, 2085
BstDSI CCRYGG 2 cut(s) 266, 687
BstF5I GGATG 1 cut(s) 453
BstH2I RGCGCY 1 cut(s) 779
BstHHI GCGC 1 cut(s) 778
BstKTI GATC 7 cut(s) 407, 576, 1099, 1328, 1534, 1614, 1986
BstMAI GTCTC 2 cut(s) 603, 1750
BstMBI GATC 7 cut(s) 404, 573, 1096, 1325, 1531, 1611, 1983
BstNI CCWGG 2 cut(s) 414, 1905
BstNSI RCATGY 1 cut(s) 1356
BstSCI CCNGG 4 cut(s) 412, 442, 1903, 2124
BstSFI CTRYAG 2 cut(s) 779, 909
BstSNI TACGTA 1 cut(s) 57
BstV1I GCAGC 7 cut(s) 73, 191, 473, 578, 592, 923, 1186
BstV2I GAAGAC 1 cut(s) 834
BstXI CCANNNNNNTGG 1 cut(s) 807
Bsu36I CCTNAGG 1 cut(s) 504
BsuRI GGCC 5 cut(s) 132, 263, 469, 1875, 2124
BtgI CCRYGG 2 cut(s) 266, 687
BtrI CACGTC 1 cut(s) 109
BtsCI GGATG 1 cut(s) 453
BtsIMutI CAGTG 5 cut(s) 294, 435, 483, 1614, 1873
BveI ACCTGC 1 cut(s) 2060
Cac8I GCNNGC 4 cut(s) 655, 659, 744, 1873
CciI TCATGA 1 cut(s) 624
CfoI GCGC 1 cut(s) 778
Cfr13I GGNCC 6 cut(s) 131, 262, 395, 440, 2072, 2123
CseI GACGC 2 cut(s) 839, 1861
CviAII CATG 6 cut(s) 267, 465, 625, 946, 1353, 1877
DdeI CTNAG 6 cut(s) 504, 554, 1260, 1335, 1583, 2085
DpnI GATC 7 cut(s) 406, 575, 1098, 1327, 1533, 1613, 1985
DpnII GATC 7 cut(s) 404, 573, 1096, 1325, 1531, 1611, 1983
DraIII CACNNNGTG 1 cut(s) 851
EaeI YGGCCR 1 cut(s) 1873
Eam1104I CTCTTC 1 cut(s) 1445
EarI CTCTTC 1 cut(s) 1445
Eco105I TACGTA 1 cut(s) 57
Eco130I CCWWGG 3 cut(s) 266, 369, 673
Eco24I GRGCYC 1 cut(s) 1911
Eco31I GGTCTC 1 cut(s) 603
Eco47I GGWCC 3 cut(s) 395, 440, 2072
Eco57I CTGAAG 2 cut(s) 837, 1029
Eco81I CCTNAGG 1 cut(s) 504
EcoO109I RGGNCCY 3 cut(s) 131, 262, 2072
EcoRI GAATTC 1 cut(s) 1207
EcoRII CCWGG 2 cut(s) 412, 1903
EcoT14I CCWWGG 3 cut(s) 266, 369, 673
EcoT38I GRGCYC 1 cut(s) 1911
ErhI CCWWGG 3 cut(s) 266, 369, 673
Esp3I CGTCTC 1 cut(s) 1750
FaeI CATG 6 cut(s) 270, 468, 628, 949, 1356, 1880
FalI AAGNNNNNCTT 2 cut(s) 753, 785
FaqI GGGAC 2 cut(s) 2058, 2074
FatI CATG 6 cut(s) 266, 464, 624, 945, 1352, 1876
FbaI TGATCA 2 cut(s) 573, 1611
Fnu4HI GCNGC 9 cut(s) 87, 180, 213, 462, 559, 567, 606, 912, 1200
FokI GGATG 1 cut(s) 440
FriOI GRGCYC 1 cut(s) 1911
Fsp4HI GCNGC 9 cut(s) 87, 180, 213, 462, 559, 567, 606, 912, 1200
FspBI CTAG 2 cut(s) 458, 752
GlaI GCGC 1 cut(s) 777
GluI GCNGC 9 cut(s) 87, 180, 213, 462, 559, 567, 606, 912, 1200
GsuI CTGGAG 1 cut(s) 585
HaeII RGCGCY 1 cut(s) 779
HaeIII GGCC 5 cut(s) 132, 263, 469, 1875, 2124
HapII CCGG 2 cut(s) 444, 2126
HgaI GACGC 2 cut(s) 839, 1861
HhaI GCGC 1 cut(s) 778
Hin1I GRCGYC 1 cut(s) 693
Hin1II CATG 6 cut(s) 270, 468, 628, 949, 1356, 1880
Hin6I GCGC 1 cut(s) 776
HinP1I GCGC 1 cut(s) 776
HincII GTYRAC 3 cut(s) 451, 1578, 1882
HindII GTYRAC 3 cut(s) 451, 1578, 1882
HinfI GANTC 7 cut(s) 271, 473, 838, 1172, 1250, 1574, 2083
HpaI GTTAAC 1 cut(s) 1882
HpaII CCGG 2 cut(s) 444, 2126
HphI GGTGA 4 cut(s) 230, 542, 952, 1981
Hpy166II GTNNAC 7 cut(s) 451, 481, 691, 993, 1016, 1578, 1882
Hpy188I TCNGA 8 cut(s) 316, 353, 874, 1261, 1584, 1750, 2014, 2082
Hpy188III TCNNGA 8 cut(s) 80, 390, 577, 625, 887, 1034, 1212, 1595
Hpy8I GTNNAC 7 cut(s) 451, 481, 691, 993, 1016, 1578, 1882
HpyAV CCTTC 5 cut(s) 1004, 1140, 1367, 1655, 2061
HpyCH4III ACNGT 4 cut(s) 280, 1826, 1868, 2002
HpyCH4IV ACGT 7 cut(s) 56, 108, 693, 985, 1758, 2118, 2138
HpyF3I CTNAG 6 cut(s) 504, 554, 1260, 1335, 1583, 2085
HpySE526I ACGT 7 cut(s) 56, 108, 693, 985, 1758, 2118, 2138
Hsp92I GRCGYC 1 cut(s) 693
Hsp92II CATG 6 cut(s) 270, 468, 628, 949, 1356, 1880
HspAI GCGC 1 cut(s) 776
KpnI GGTACC 1 cut(s) 534
Ksp22I TGATCA 2 cut(s) 573, 1611
KspAI GTTAAC 1 cut(s) 1882
Kzo9I GATC 7 cut(s) 404, 573, 1096, 1325, 1531, 1611, 1983
LguI GCTCTTC 1 cut(s) 1445
LmnI GCTCC 3 cut(s) 227, 284, 370
Lsp1109I GCAGC 7 cut(s) 73, 191, 473, 578, 592, 923, 1186
LweI GCATC 3 cut(s) 73, 346, 729
MaeI CTAG 2 cut(s) 458, 752
MaeII ACGT 7 cut(s) 56, 108, 693, 985, 1758, 2118, 2138
MalI GATC 7 cut(s) 406, 575, 1098, 1327, 1533, 1613, 1985
MboI GATC 7 cut(s) 404, 573, 1096, 1325, 1531, 1611, 1983
MfeI CAATTG 1 cut(s) 702
MhlI GDGCHC 2 cut(s) 847, 1911
MlsI TGGCCA 1 cut(s) 1875
MluNI TGGCCA 1 cut(s) 1875
MlyI GAGTC 2 cut(s) 1583, 2077
MmeI TCCRAC 4 cut(s) 262, 695, 912, 1999
Mox20I TGGCCA 1 cut(s) 1875
MroXI GAANNNNTTC 1 cut(s) 882
MscI TGGCCA 1 cut(s) 1875
MslI CAYNNNNRTG 2 cut(s) 1035, 1866
Msp20I TGGCCA 1 cut(s) 1875
MspA1I CMGCKG 4 cut(s) 207, 605, 635, 914
MspCI CTTAAG 2 cut(s) 770, 926
MspI CCGG 2 cut(s) 444, 2126
MspR9I CCNGG 4 cut(s) 414, 444, 1905, 2126
MunI CAATTG 1 cut(s) 702
MvaI CCWGG 2 cut(s) 414, 1905
NciI CCSGG 2 cut(s) 444, 2126
NcoI CCATGG 1 cut(s) 266
NdeII GATC 7 cut(s) 404, 573, 1096, 1325, 1531, 1611, 1983
NlaIII CATG 6 cut(s) 270, 468, 628, 949, 1356, 1880
NlaIV GGNNCC 6 cut(s) 6, 133, 264, 442, 532, 2074
NspI RCATGY 1 cut(s) 1356
OliI CACNNNNGTG 1 cut(s) 1866
PagI TCATGA 1 cut(s) 624
PasI CCCWGGG 1 cut(s) 1904
PciSI GCTCTTC 1 cut(s) 1445
PdmI GAANNNNTTC 1 cut(s) 882
PfeI GAWTC 5 cut(s) 271, 473, 838, 1172, 1250
Pfl23II CGTACG 1 cut(s) 57
PflFI GACNNNGTC 1 cut(s) 2086
PkrI GCNGC 9 cut(s) 88, 181, 214, 463, 560, 568, 607, 913, 1201
PleI GAGTC 2 cut(s) 1582, 2077
PpsI GAGTC 2 cut(s) 1582, 2077
Ppu21I YACGTR 1 cut(s) 57
PpuMI RGGWCCY 1 cut(s) 2072
PshBI ATTAAT 1 cut(s) 1086
Psp1406I AACGTT 2 cut(s) 985, 2118
Psp5II RGGWCCY 1 cut(s) 2072
Psp6I CCWGG 2 cut(s) 412, 1903
PspGI CCWGG 2 cut(s) 412, 1903
PspLI CGTACG 1 cut(s) 57
PspN4I GGNNCC 6 cut(s) 6, 133, 264, 442, 532, 2074
PspPI GGNCC 6 cut(s) 131, 262, 395, 440, 2072, 2123
PspPPI RGGWCCY 1 cut(s) 2072
PstI CTGCAG 1 cut(s) 913
PsyI GACNNNGTC 1 cut(s) 2086
PvuII CAGCTG 3 cut(s) 207, 605, 914
RseI CAYNNNNRTG 2 cut(s) 1035, 1866
SapI GCTCTTC 1 cut(s) 1445
SatI GCNGC 9 cut(s) 87, 180, 213, 462, 559, 567, 606, 912, 1200
Sau3AI GATC 7 cut(s) 404, 573, 1096, 1325, 1531, 1611, 1983
Sau96I GGNCC 6 cut(s) 131, 262, 395, 440, 2072, 2123
ScaI AGTACT 1 cut(s) 2096
SchI GAGTC 2 cut(s) 1583, 2077
ScrFI CCNGG 4 cut(s) 414, 444, 1905, 2126
SduI GDGCHC 2 cut(s) 847, 1911
SfaNI GCATC 3 cut(s) 73, 346, 729
SfcI CTRYAG 2 cut(s) 779, 909
SinI GGWCC 3 cut(s) 395, 440, 2072
SmiMI CAYNNNNRTG 2 cut(s) 1035, 1866
SmlI CTYRAG 2 cut(s) 770, 926
SmoI CTYRAG 2 cut(s) 770, 926
SnaBI TACGTA 1 cut(s) 57
SsiI CCGC 5 cut(s) 212, 558, 633, 1859, 1953
SspI AATATT 1 cut(s) 198
SspMI CTAG 2 cut(s) 458, 752
StyD4I CCNGG 4 cut(s) 412, 442, 1903, 2124
StyI CCWWGG 3 cut(s) 266, 369, 673
TaaI ACNGT 4 cut(s) 280, 1826, 1868, 2002
TaiI ACGT 7 cut(s) 59, 111, 696, 988, 1761, 2121, 2141
TaqI TCGA 3 cut(s) 841, 1328, 1917
TatI WGTACW 5 cut(s) 27, 640, 990, 1633, 2094
TauI GCSGC 2 cut(s) 215, 561
TfiI GAWTC 5 cut(s) 271, 473, 838, 1172, 1250
TscAI CASTG 5 cut(s) 301, 442, 483, 1621, 1873
TseI GCWGC 7 cut(s) 86, 179, 461, 566, 605, 911, 1199
TspDTI ATGAA 6 cut(s) 891, 915, 1432, 1833, 1983, 2043
TspGWI ACGGA 2 cut(s) 240, 989
TspRI CASTG 5 cut(s) 301, 442, 483, 1621, 1873
Tth111I GACNNNGTC 1 cut(s) 2086
Vha464I CTTAAG 2 cut(s) 770, 926
VpaK11BI GGWCC 3 cut(s) 395, 440, 2072
VspI ATTAAT 1 cut(s) 1086
XapI RAATTY 8 cut(s) 417, 1141, 1207, 1240, 1318, 1598, 1665, 1732
XceI RCATGY 1 cut(s) 1356
XcmI CCANNNNNNNNNTGG 1 cut(s) 1517
XmnI GAANNNNTTC 1 cut(s) 882
XspI CTAG 2 cut(s) 458, 752
ZraI GACGTC 1 cut(s) 694
ZrmI AGTACT 1 cut(s) 2096
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.