FvH4_4g02061

Pfam:UBN2_2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000278
Physical Location & Seq
Forward (+)
1825007 .. 1826123
1117 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g02061.t1

Sequence Viewer

Length: 789 bp
ATGGACGAAGAGAAGAACAAGAAAGCAAAGAGCTGTATTGAGTTGCATCTTGCTGATAATGTCTTACTTCACATTGGAGAGACAATGACTGCAAATGAAGCATGGGAAAAGCTTGAAAGCGTCTACAGGGGTAAAAGCATCGGCAACAAGCTACTCTTGAAGGAACAGCTCTTTGGCCTCAGGATGGAGGAAGGGGATGATCTTAATGATCATATATGCAAGTTCCAGAACTGCATAGCAAACTTGGAGAGAAGGAATTGCAAACAATGGAAGGAGAAGAAGGCGCAGATGTCAGGAGAAAGCTCCCAGTCAGCCAACGTCGTCATTGGATACAATGATGAGGATGGAGAACTCCTCGCAATCTCAACCAGCTCCGGCGCGCCTAGACATTGGACCTTGGATACGGCCTGCACGTTTCATATGTGTGCACACAGAGATTGGTTCAACACATATAAGGAGAGGAACACCGGATCAGTTTTGATGGGAAATGATTCACCAAGCAGAATTATGGGAATCGGAACAGTAAGGATCAGAATGCATGATGGCATTGTCAGAACGCTGGGAAACGTTAGACATATCCCAGGTTTGAGCAGAAATCTGATTTCATTAACTACCATGGATAGGGCTGGATTTTGGCATAAAGGTCAGAATGGAGTGCTCAAAGTAGGAAAGGGACAAATGGTCTACATGAAGGGTGCAATACAACCAGATAACATGTATAAACTCACAGGTTCTACAGTAGAAGGTGGAGCAGCAGTTTGTACGGAAGAAGACAAGACTGAGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

263

Amino Acids

29.31

Weight (kDa)

7.11

Isoelectric Point (pI)

43.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_2 PF14223 4 - 92 9.3e-19 gag-polypeptide of LTR copia-type
Pol_BBD PF22936 131 - 211 4.5e-27 Pol polyprotein, beta-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000278)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G29785
fragaria_vesca FvH4_1g10046 FvH4_1g17351 FvH4_1g18441 FvH4_1g24222 FvH4_2g00141 FvH4_2g05931 FvH4_2g06671 FvH4_2g14030 FvH4_2g15291 FvH4_2g22611 FvH4_2g22612 FvH4_2g26380 FvH4_2g39202 FvH4_2g39202 FvH4_3g06691 FvH4_3g08580 FvH4_3g20502 FvH4_3g20503 FvH4_3g22421 FvH4_3g42580 FvH4_4g02061 FvH4_4g06794 FvH4_4g08661 FvH4_4g11771 FvH4_4g14601 FvH4_4g14721 FvH4_4g20921 FvH4_4g33601 FvH4_4g33602 FvH4_5g07492 FvH4_5g20321 FvH4_5g21591 FvH4_5g30343 FvH4_5g37875 FvH4_6g04372 FvH4_6g04373 FvH4_6g04373 FvH4_6g21377 FvH4_6g23760 FvH4_6g29471 FvH4_6g43142 FvH4_7g14351 FvH4_7g14352 FvH4_7g22582 FvH4_7g22583 FvH4_7g22584
malus_domestica MD00G1179400.v1.1 MD01G1170500.v1.1 MD03G1286700.v1.1 MD04G1054600.v1.1 MD04G1071700.v1.1 MD04G1193900.v1.1 MD08G1054300.v1.1 MD08G1217500.v1.1 MD09G1034600.v1.1 MD09G1098100.v1.1 MD10G1018300.v1.1 MD10G1220000.v1.1 MD10G1250300.v1.1 MD12G1014400.v1.1 MD15G1021000.v1.1 MD16G1112200.v1.1
prunus_persica Prupe.1G188900_v2.0.a1 Prupe.1G258000_v2.0.a1 Prupe.4G233400_v2.0.a1
pyrus_communis pycom01g10100 pycom02g01340 pycom03g19700 pycom05g01320 pycom07g16000 pycom07g16520 pycom11g26500 pycom13g09380 pycom13g13960 pycom14g08180 pycom14g16810 pycom16g09590
rosa_chinensis RchiOBHm_Chr1g0319891 RchiOBHm_Chr1g0333711 RchiOBHm_Chr2g0115331 RchiOBHm_Chr2g0143411 RchiOBHm_Chr2g0167631 RchiOBHm_Chr3g0448581 RchiOBHm_Chr3g0494991 RchiOBHm_Chr4g0442471 RchiOBHm_Chr6g0262311 RchiOBHm_Chr6g0301831 RchiOBHm_Chr6g0301841 RchiOBHm_Chr7g0183451
rosa_roxburghii Rroxscaffold_1G00039280 Rroxscaffold_1G00047670 Rroxscaffold_1G00066330 Rroxscaffold_2G00107510 Rroxscaffold_3G00255410 Rroxscaffold_4G00301460 Rroxscaffold_5G00352730 Rroxscaffold_7G00166350 Rroxscaffold_7G00166360 Rroxscaffold_7G00203820
rosa_rugosa Rorug03G0240500 Rorug05G0191700 Rorug06G0310200
rosa_samantha Rh2BG287000 Rh4CG073500 Rh4CG176200 Rh4CG191400 Rh5BG356100 Rh5BG434100 Rh6AG110600 Rh6AG421900 Rh6AG422000 Rh6AG422100 Rh6BG428600 Rh6BG428700 Rh6BG428800 Rh6CG435200 Rh6CG435300 Rh6CG435400 Rh6DG422000 Rh6DG422100 Rh6DG422200 Rh7BG190500 Rh7CG172800 Rh7CG225200
rosa_wichuraiana Rw2G044060 Rw6G036540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 123, 684
AccII CGCG 1 cut(s) 380
AclI AACGTT 1 cut(s) 567
AclWI GGATC 2 cut(s) 478, 536
AfaI GTAC 1 cut(s) 763
AfiI CCNNNNNNNGG 2 cut(s) 184, 621
AflIII ACRYGT 1 cut(s) 714
AgsI TTSAA 3 cut(s) 116, 160, 445
AhdI GACNNNNNGTC 1 cut(s) 680
AjnI CCWGG 1 cut(s) 580
AjuI GAANNNNNNNTTGG 2 cut(s) 156, 188
AluBI AGCT 7 cut(s) 33, 112, 151, 169, 303, 372, 784
AluI AGCT 7 cut(s) 33, 112, 151, 169, 303, 372, 784
Alw21I GWGCWC 2 cut(s) 430, 660
Alw26I GTCTC 1 cut(s) 74
Alw44I GTGCAC 1 cut(s) 426
AlwI GGATC 2 cut(s) 478, 536
AoxI GGCC 2 cut(s) 175, 405
ApaLI GTGCAC 1 cut(s) 426
ApeKI GCWGC 1 cut(s) 752
AscI GGCGCGCC 1 cut(s) 378
Asp700I GAANNNNTTC 1 cut(s) 490
AspLEI GCGC 3 cut(s) 286, 380, 382
AspS9I GGNCC 1 cut(s) 393
AsuHPI GGTGA 1 cut(s) 486
AvaII GGWCC 1 cut(s) 393
AxyI CCTNAGG 1 cut(s) 179
BaeGI GKGCMC 1 cut(s) 430
BbsI GAAGAC 1 cut(s) 777
Bbv12I GWGCWC 2 cut(s) 430, 660
BbvI GCAGC 1 cut(s) 764
BccI CCATC 4 cut(s) 178, 338, 475, 536
BceAI ACGGC 1 cut(s) 420
BciT130I CCWGG 1 cut(s) 582
BciVI GTATCC 2 cut(s) 323, 394
BclI TGATCA 1 cut(s) 208
BcoDI GTCTC 1 cut(s) 74
BfaI CTAG 1 cut(s) 384
BfmI CTRYAG 2 cut(s) 124, 735
BfuI GTATCC 2 cut(s) 323, 394
BisI GCNGC 1 cut(s) 753
BlsI GCNGC 1 cut(s) 754
Bme1390I CCNGG 1 cut(s) 582
Bme18I GGWCC 1 cut(s) 393
BmeRI GACNNNNNGTC 1 cut(s) 680
BmgT120I GGNCC 1 cut(s) 393
BmrFI CCNGG 1 cut(s) 582
BmrI ACTGGG 1 cut(s) 301
BmsI GCATC 2 cut(s) 55, 147
BmuI ACTGGG 1 cut(s) 301
BpiI GAAGAC 1 cut(s) 777
BplI GAGNNNNNCTC 2 cut(s) 339, 371
BsaJI CCNNGG 3 cut(s) 396, 580, 615
BsaWI WCCGGW 1 cut(s) 467
BsaXI ACNNNNNCTCC 2 cut(s) 741, 771
Bsc4I CCNNNNNNNGG 2 cut(s) 184, 621
Bse1I ACTGG 1 cut(s) 307
Bse21I CCTNAGG 1 cut(s) 179
BseBI CCWGG 1 cut(s) 582
BseDI CCNNGG 3 cut(s) 396, 580, 615
BseGI GGATG 3 cut(s) 189, 202, 349
BseLI CCNNNNNNNGG 2 cut(s) 184, 621
BseMII CTCAG 2 cut(s) 193, 771
BseNI ACTGG 1 cut(s) 307
BsePI GCGCGC 1 cut(s) 378
BseRI GAGGAG 1 cut(s) 344
BseSI GKGCMC 1 cut(s) 430
BseXI GCAGC 1 cut(s) 764
BseYI CCCAGC 1 cut(s) 559
BsgI GTGCAG 1 cut(s) 394
Bsh1236I CGCG 1 cut(s) 380
BshFI GGCC 2 cut(s) 177, 407
BsiHKAI GWGCWC 2 cut(s) 430, 660
BsiSI CCGG 2 cut(s) 375, 468
BslFI GGGAC 1 cut(s) 687
BslI CCNNNNNNNGG 2 cut(s) 184, 621
BsmAI GTCTC 1 cut(s) 74
BsmFI GGGAC 1 cut(s) 687
BsmI GAATGC 1 cut(s) 540
BsnI GGCC 2 cut(s) 177, 407
Bsp1286I GDGCHC 2 cut(s) 430, 660
Bsp143I GATC 4 cut(s) 199, 208, 470, 528
Bsp19I CCATGG 1 cut(s) 615
BspANI GGCC 2 cut(s) 177, 407
BspCNI CTCAG 2 cut(s) 192, 772
BspFNI CGCG 1 cut(s) 380
BspPI GGATC 2 cut(s) 478, 536
BsrI ACTGG 1 cut(s) 307
BssECI CCNNGG 3 cut(s) 396, 580, 615
BssHII GCGCGC 1 cut(s) 378
BssMI GATC 4 cut(s) 199, 208, 470, 528
BssT1I CCWWGG 2 cut(s) 396, 615
Bst2UI CCWGG 1 cut(s) 582
Bst4CI ACNGT 2 cut(s) 523, 739
Bst6I CTCTTC 1 cut(s) 3
BstC8I GCNNGC 2 cut(s) 380, 409
BstDEI CTNAG 2 cut(s) 179, 780
BstDSI CCRYGG 1 cut(s) 615
BstF5I GGATG 3 cut(s) 189, 202, 349
BstFNI CGCG 1 cut(s) 380
BstHHI GCGC 3 cut(s) 286, 380, 382
BstKTI GATC 4 cut(s) 202, 211, 473, 531
BstMAI GTCTC 1 cut(s) 74
BstMBI GATC 4 cut(s) 199, 208, 470, 528
BstMWI GCNNNNNNNGC 1 cut(s) 98
BstNI CCWGG 1 cut(s) 582
BstNSI RCATGY 1 cut(s) 718
BstSCI CCNGG 1 cut(s) 580
BstSFI CTRYAG 2 cut(s) 124, 735
BstSLI GKGCMC 1 cut(s) 430
BstUI CGCG 1 cut(s) 380
BstV1I GCAGC 1 cut(s) 764
BstV2I GAAGAC 1 cut(s) 777
Bsu36I CCTNAGG 1 cut(s) 179
BsuI GTATCC 2 cut(s) 323, 394
BsuRI GGCC 2 cut(s) 177, 407
BtgI CCRYGG 1 cut(s) 615
BtsCI GGATG 3 cut(s) 189, 202, 349
Cac8I GCNNGC 2 cut(s) 380, 409
CfoI GCGC 3 cut(s) 286, 380, 382
Cfr13I GGNCC 1 cut(s) 393
CseI GACGC 1 cut(s) 109
Csp6I GTAC 1 cut(s) 762
CviAII CATG 5 cut(s) 102, 539, 616, 688, 715
CviQI GTAC 1 cut(s) 762
DdeI CTNAG 2 cut(s) 179, 780
DpnI GATC 4 cut(s) 201, 210, 472, 530
DpnII GATC 4 cut(s) 199, 208, 470, 528
DriI GACNNNNNGTC 1 cut(s) 680
Eam1104I CTCTTC 1 cut(s) 3
Eam1105I GACNNNNNGTC 1 cut(s) 680
EarI CTCTTC 1 cut(s) 3
Eco130I CCWWGG 2 cut(s) 396, 615
Eco47I GGWCC 1 cut(s) 393
Eco81I CCTNAGG 1 cut(s) 179
EcoRII CCWGG 1 cut(s) 580
EcoT14I CCWWGG 2 cut(s) 396, 615
EcoT22I ATGCAT 1 cut(s) 540
ErhI CCWWGG 2 cut(s) 396, 615
FaeI CATG 5 cut(s) 105, 542, 619, 691, 718
FalI AAGNNNNNCTT 2 cut(s) 140, 172
FaqI GGGAC 1 cut(s) 687
FatI CATG 5 cut(s) 101, 538, 615, 687, 714
FauNDI CATATG 1 cut(s) 420
FbaI TGATCA 1 cut(s) 208
FblI GTMKAC 2 cut(s) 123, 684
Fnu4HI GCNGC 1 cut(s) 753
FokI GGATG 3 cut(s) 196, 209, 356
Fsp4HI GCNGC 1 cut(s) 753
FspBI CTAG 1 cut(s) 384
GlaI GCGC 3 cut(s) 285, 379, 381
GluI GCNGC 1 cut(s) 753
GsaI CCCAGC 1 cut(s) 563
HaeIII GGCC 2 cut(s) 177, 407
HapII CCGG 2 cut(s) 375, 468
HgaI GACGC 1 cut(s) 109
HhaI GCGC 3 cut(s) 286, 380, 382
Hin1II CATG 5 cut(s) 105, 542, 619, 691, 718
Hin6I GCGC 3 cut(s) 284, 378, 380
HinP1I GCGC 3 cut(s) 284, 378, 380
HindIII AAGCTT 1 cut(s) 110
HinfI GANTC 2 cut(s) 491, 513
HpaII CCGG 2 cut(s) 375, 468
HphI GGTGA 1 cut(s) 486
Hpy166II GTNNAC 3 cut(s) 124, 428, 685
Hpy188I TCNGA 5 cut(s) 518, 533, 554, 600, 648
Hpy188III TCNNGA 4 cut(s) 157, 181, 226, 294
Hpy8I GTNNAC 3 cut(s) 124, 428, 685
Hpy99I CGWCG 1 cut(s) 323
HpyAV CCTTC 7 cut(s) 154, 185, 246, 265, 274, 685, 737
HpyCH4III ACNGT 2 cut(s) 523, 739
HpyCH4IV ACGT 3 cut(s) 318, 413, 567
HpyCH4V TGCA 9 cut(s) 46, 92, 219, 234, 261, 411, 428, 538, 698
HpyF10VI GCNNNNNNNGC 1 cut(s) 98
HpyF3I CTNAG 2 cut(s) 179, 780
HpySE526I ACGT 3 cut(s) 318, 413, 567
Hsp92II CATG 5 cut(s) 105, 542, 619, 691, 718
HspAI GCGC 3 cut(s) 284, 378, 380
Ksp22I TGATCA 1 cut(s) 208
Kzo9I GATC 4 cut(s) 199, 208, 470, 528
LmnI GCTCC 3 cut(s) 308, 377, 749
Lsp1109I GCAGC 1 cut(s) 764
LweI GCATC 2 cut(s) 55, 147
MaeI CTAG 1 cut(s) 384
MaeII ACGT 3 cut(s) 318, 413, 567
MalI GATC 4 cut(s) 201, 210, 472, 530
MboI GATC 4 cut(s) 199, 208, 470, 528
MboII GAAGA 5 cut(s) 20, 25, 289, 779, 782
MhlI GDGCHC 2 cut(s) 430, 660
MluCI AATT 2 cut(s) 256, 504
MnlI CCTC 5 cut(s) 181, 188, 334, 365, 453
Mph1103I ATGCAT 1 cut(s) 540
MroXI GAANNNNTTC 1 cut(s) 490
MseI TTAA 2 cut(s) 204, 608
MslI CAYNNNNRTG 1 cut(s) 423
MspI CCGG 2 cut(s) 375, 468
MspR9I CCNGG 1 cut(s) 582
Mva1269I GAATGC 1 cut(s) 540
MvaI CCWGG 1 cut(s) 582
MvnI CGCG 1 cut(s) 380
MwoI GCNNNNNNNGC 1 cut(s) 98
NcoI CCATGG 1 cut(s) 615
NdeI CATATG 1 cut(s) 420
NdeII GATC 4 cut(s) 199, 208, 470, 528
NlaIII CATG 5 cut(s) 105, 542, 619, 691, 718
NsiI ATGCAT 1 cut(s) 540
NspI RCATGY 1 cut(s) 718
PalAI GGCGCGCC 1 cut(s) 378
PauI GCGCGC 1 cut(s) 378
PciI ACATGT 1 cut(s) 714
PcsI WCGNNNNNNNCGW 1 cut(s) 410
PctI GAATGC 1 cut(s) 540
PdmI GAANNNNTTC 1 cut(s) 490
PfeI GAWTC 2 cut(s) 491, 513
PkrI GCNGC 1 cut(s) 754
PscI ACATGT 1 cut(s) 714
Psp1406I AACGTT 1 cut(s) 567
Psp6I CCWGG 1 cut(s) 580
PspFI CCCAGC 1 cut(s) 559
PspGI CCWGG 1 cut(s) 580
PspPI GGNCC 1 cut(s) 393
PteI GCGCGC 1 cut(s) 378
RsaI GTAC 1 cut(s) 763
RsaNI GTAC 1 cut(s) 762
RseI CAYNNNNRTG 1 cut(s) 423
SaqAI TTAA 2 cut(s) 204, 608
SatI GCNGC 1 cut(s) 753
Sau3AI GATC 4 cut(s) 199, 208, 470, 528
Sau96I GGNCC 1 cut(s) 393
ScrFI CCNGG 1 cut(s) 582
SduI GDGCHC 2 cut(s) 430, 660
SfaNI GCATC 2 cut(s) 55, 147
SfcI CTRYAG 2 cut(s) 124, 735
SgsI GGCGCGCC 1 cut(s) 378
SinI GGWCC 1 cut(s) 393
SmiMI CAYNNNNRTG 1 cut(s) 423
Sse9I AATT 2 cut(s) 256, 504
SspMI CTAG 1 cut(s) 384
StyD4I CCNGG 1 cut(s) 580
StyI CCWWGG 2 cut(s) 396, 615
TaaI ACNGT 2 cut(s) 523, 739
TaiI ACGT 3 cut(s) 321, 416, 570
TasI AATT 2 cut(s) 256, 504
TfiI GAWTC 2 cut(s) 491, 513
Tru1I TTAA 2 cut(s) 204, 608
Tru9I TTAA 2 cut(s) 204, 608
TseI GCWGC 1 cut(s) 752
TspDTI ATGAA 4 cut(s) 111, 407, 594, 704
TspGWI ACGGA 1 cut(s) 779
VneI GTGCAC 1 cut(s) 426
VpaK11BI GGWCC 1 cut(s) 393
XceI RCATGY 1 cut(s) 718
XmiI GTMKAC 2 cut(s) 123, 684
XmnI GAANNNNTTC 1 cut(s) 490
XspI CTAG 1 cut(s) 384
Zsp2I ATGCAT 1 cut(s) 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.