FvH4_2g14331

Protein of unknown function (DUF 659)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000421
Physical Location & Seq
Reverse (-)
12599616 .. 12600383
768 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g14331.t1

Sequence Viewer

Length: 534 bp
ATGTCGCCTTTCCCTGTCAGTGCGATTTTTCCTCCACTGATGCCGCTCTGCGAGAACGACGAGAACGGCCCTGCGACTCTTCCTCTTCGCATAGAAGAGGAACGACGAAAAAGGCAACCGAGGAACGACATCGCCGTTCTCTGTCACTGCGAGAACGATGTCGCCTTTCCCTGTCAGTGCGATTTTTCCTCCACCGATGCCGCTCTGCGAGAACGACGAGAACTGCCCTGTGCGCCTTTCTCTGTCACAGCGACTCTTCCTCCGCTGACGTCGCCTTCGCCGACGTCGCCTCCGCCTATAGCCGTCAGCGGCCTAAACGCTCTCTTTGGTTTAGTTTATGTCCATACCAATCTACGTCTTTTAGCAAGAAAGAGCTCTAGCTATCACAAAGATAAGGACTCAATGTGGGATCTTAGAAGAGATGACCATGACTCCATGGAACAAGGCAATATTGATGTGCTTGAGATGGCGACTTTGTCGCTTGATGTGCCGGCCTTGGAGAGGATGGTAATTGATGATGAGATGGAGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT

Protein Analysis

178

Amino Acids

19.78

Weight (kDa)

4.72

Isoelectric Point (pI)

77.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000421)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08267
fragaria_vesca FvH4_2g14331 FvH4_2g16371 FvH4_2g26163 FvH4_3g42540 FvH4_4g02102 FvH4_4g12491 FvH4_4g35171 FvH4_5g06632 FvH4_5g06632 FvH4_5g06632 FvH4_5g08811 FvH4_5g08812 FvH4_5g23531 FvH4_6g10272 FvH4_6g40844 FvH4_7g16803 FvH4_7g16804
malus_domestica MD11G1099500.v1.1 MD17G1176800.v1.1
pyrus_communis pycom05g07840 pycom06g08730 pycom07g12010 pycom07g12020 pycom10g07270 pycom11g17470 pycom12g10610 pycom12g14240 pycom13g22040 pycom15g02440
rosa_chinensis RchiOBHm_Chr1g0371321 RchiOBHm_Chr2g0102701 RchiOBHm_Chr3g0465681 RchiOBHm_Chr4g0420941 RchiOBHm_Chr4g0434271 RchiOBHm_Chr4g0442721 RchiOBHm_Chr6g0262081 RchiOBHm_Chr6g0278171 RchiOBHm_Chr6g0294991 RchiOBHm_Chr6g0295001 RchiOBHm_Chr7g0205361 RchiOBHm_Chr7g0205371 RchiOBHm_Chr7g0223471
rosa_laevigata RLG00000005266
rosa_multiflora Rmu_sc0000493.1_g000033 Rmu_sc0000493.1_g000034 Rmu_sc0000507.1_g000007 Rmu_sc0000843.1_g000001 Rmu_sc0001075.1_g000033 Rmu_sc0002077.1_g000007 Rmu_sc0002077.1_g000008 Rmu_sc0002329.1_g000029 Rmu_sc0002539.1_g000077 Rmu_sc0002655.1_g000006 Rmu_sc0003127.1_g000020 Rmu_sc0003765.1_g000054 Rmu_sc0004368.1_g000023 Rmu_sc0004753.1_g000022 Rmu_sc0005297.1_g000014 Rmu_sc0007633.1_g000010 Rmu_sc0008164.1_g000005 Rmu_sc0008191.1_g000003 Rmu_sc0017900.1_g000005 Rmu_sc0022727.1_g000002
rosa_roxburghii Rroxscaffold_2G00079920 Rroxscaffold_3G00255890 Rroxscaffold_4G00295340 Rroxscaffold_4G00296360 Rroxscaffold_4G00299640 Rroxscaffold_6G00388210
rosa_rugosa Rorug02G0466700 Rorug04G0123200 Rorug05G0074800 Rorug07G0202900
rosa_samantha Rh1DG134700 Rh3CG159000 Rh4AG331000 Rh5AG521100 Rh5BG136400 Rh5DG127300 Rh5DG383100 Rh5DG386100 Rh5DG458000 Rh6CG450400 Rh7CG233800 Rh7DG407100
rosa_wichuraiana Rw1G004960 Rw1G005730 Rw1G006360 Rw1G017400 Rw2G013190 Rw2G022210 Rw2G029640 Rw5G020910 Rw5G036930 Rw6G016200 Rw7G018170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 272, 287
AccBSI CCGCTC 2 cut(s) 46, 203
AciI CCGC 5 cut(s) 44, 201, 263, 293, 309
AclWI GGATC 1 cut(s) 417
AcyI GRCGYC 2 cut(s) 269, 284
AfiI CCNNNNNNNGG 1 cut(s) 501
AluBI AGCT 2 cut(s) 375, 381
AluI AGCT 2 cut(s) 375, 381
Alw21I GWGCWC 1 cut(s) 377
Alw26I GTCTC 1 cut(s) 521
AlwI GGATC 1 cut(s) 417
AoxI GGCC 3 cut(s) 67, 310, 492
ArsI GACNNNNNNTTYG 2 cut(s) 259, 291
AspLEI GCGC 1 cut(s) 235
AspS9I GGNCC 1 cut(s) 68
BanII GRGCYC 1 cut(s) 377
Bbv12I GWGCWC 1 cut(s) 377
BccI CCATC 3 cut(s) 460, 499, 517
BceAI ACGGC 3 cut(s) 82, 119, 287
BcoDI GTCTC 1 cut(s) 521
BfaI CTAG 1 cut(s) 378
BfmI CTRYAG 1 cut(s) 297
BisI GCNGC 3 cut(s) 44, 201, 310
BlsI GCNGC 3 cut(s) 45, 202, 311
BmgT120I GGNCC 1 cut(s) 68
BmsI GCATC 2 cut(s) 30, 187
BpuEI CTTGAG 1 cut(s) 482
BsaHI GRCGYC 2 cut(s) 269, 284
BsaJI CCNNGG 3 cut(s) 119, 435, 495
BsaXI ACNNNNNCTCC 6 cut(s) 244, 274, 274, 304, 416, 446
Bsc4I CCNNNNNNNGG 1 cut(s) 501
Bse118I RCCGGY 1 cut(s) 490
BseDI CCNNGG 3 cut(s) 119, 435, 495
BseGI GGATG 1 cut(s) 510
BseLI CCNNNNNNNGG 1 cut(s) 501
BshFI GGCC 3 cut(s) 69, 312, 494
BsiHKAI GWGCWC 1 cut(s) 377
BsiSI CCGG 1 cut(s) 491
BslI CCNNNNNNNGG 1 cut(s) 501
BsmAI GTCTC 1 cut(s) 521
BsnI GGCC 3 cut(s) 69, 312, 494
Bsp1286I GDGCHC 1 cut(s) 377
Bsp143I GATC 1 cut(s) 409
Bsp19I CCATGG 1 cut(s) 435
BspACI CCGC 5 cut(s) 44, 201, 263, 293, 309
BspANI GGCC 3 cut(s) 69, 312, 494
BspPI GGATC 1 cut(s) 417
BsrBI CCGCTC 2 cut(s) 46, 203
BsrFI RCCGGY 1 cut(s) 490
BssAI RCCGGY 1 cut(s) 490
BssECI CCNNGG 3 cut(s) 119, 435, 495
BssMI GATC 1 cut(s) 409
BssNI GRCGYC 2 cut(s) 269, 284
BssT1I CCWWGG 2 cut(s) 435, 495
Bst6I CTCTTC 5 cut(s) 84, 90, 90, 261, 412
BstACI GRCGYC 2 cut(s) 269, 284
BstC8I GCNNGC 1 cut(s) 492
BstDEI CTNAG 1 cut(s) 413
BstDSI CCRYGG 1 cut(s) 435
BstENI CCTNNNNNAGG 1 cut(s) 499
BstF5I GGATG 1 cut(s) 510
BstHHI GCGC 1 cut(s) 235
BstKTI GATC 1 cut(s) 412
BstMAI GTCTC 1 cut(s) 521
BstMBI GATC 1 cut(s) 409
BstMWI GCNNNNNNNGC 4 cut(s) 232, 271, 286, 487
BstSFI CTRYAG 1 cut(s) 297
BstX2I RGATCY 1 cut(s) 409
BstYI RGATCY 1 cut(s) 409
BsuRI GGCC 3 cut(s) 69, 312, 494
BtgI CCRYGG 1 cut(s) 435
BtgZI GCGATG 1 cut(s) 115
BtsCI GGATG 1 cut(s) 510
BtsI GCAGTG 1 cut(s) 145
BtsIMutI CAGTG 4 cut(s) 25, 35, 145, 182
Cac8I GCNNGC 1 cut(s) 492
CfoI GCGC 1 cut(s) 235
Cfr10I RCCGGY 1 cut(s) 490
Cfr13I GGNCC 1 cut(s) 68
CviAII CATG 2 cut(s) 428, 436
CviJI RGCY 6 cut(s) 69, 302, 312, 375, 381, 494
CviKI_1 RGCY 6 cut(s) 69, 302, 312, 375, 381, 494
DdeI CTNAG 1 cut(s) 413
DpnI GATC 1 cut(s) 411
DpnII GATC 1 cut(s) 409
Eam1104I CTCTTC 5 cut(s) 84, 90, 90, 261, 412
EarI CTCTTC 5 cut(s) 84, 90, 90, 261, 412
EciI GGCGGA 1 cut(s) 282
Ecl136II GAGCTC 1 cut(s) 375
Eco130I CCWWGG 2 cut(s) 435, 495
Eco24I GRGCYC 1 cut(s) 377
Eco53kI GAGCTC 1 cut(s) 375
EcoICRI GAGCTC 1 cut(s) 375
EcoNI CCTNNNNNAGG 1 cut(s) 499
EcoT14I CCWWGG 2 cut(s) 435, 495
EcoT38I GRGCYC 1 cut(s) 377
ErhI CCWWGG 2 cut(s) 435, 495
FaeI CATG 2 cut(s) 431, 439
FaiI YATR 6 cut(s) 92, 299, 339, 345, 429, 437
FatI CATG 2 cut(s) 427, 435
Fnu4HI GCNGC 3 cut(s) 44, 201, 310
FokI GGATG 1 cut(s) 517
FriOI GRGCYC 1 cut(s) 377
Fsp4HI GCNGC 3 cut(s) 44, 201, 310
FspBI CTAG 1 cut(s) 378
GlaI GCGC 1 cut(s) 234
GluI GCNGC 3 cut(s) 44, 201, 310
HaeIII GGCC 3 cut(s) 69, 312, 494
HapII CCGG 1 cut(s) 491
HhaI GCGC 1 cut(s) 235
Hin1I GRCGYC 2 cut(s) 269, 284
Hin1II CATG 2 cut(s) 431, 439
Hin6I GCGC 1 cut(s) 233
HinP1I GCGC 1 cut(s) 233
HinfI GANTC 4 cut(s) 76, 253, 398, 431
HpaII CCGG 1 cut(s) 491
Hpy99I CGWCG 6 cut(s) 62, 108, 219, 274, 286, 289
HpyAV CCTTC 1 cut(s) 285
HpyCH4IV ACGT 3 cut(s) 269, 284, 355
HpyF10VI GCNNNNNNNGC 4 cut(s) 232, 271, 286, 487
HpyF3I CTNAG 1 cut(s) 413
HpySE526I ACGT 3 cut(s) 269, 284, 355
Hsp92I GRCGYC 2 cut(s) 269, 284
Hsp92II CATG 2 cut(s) 431, 439
HspAI GCGC 1 cut(s) 233
KroI GCCGGC 1 cut(s) 490
KroNI GCCGGC 1 cut(s) 492
Kzo9I GATC 1 cut(s) 409
LpnPI CCDG 5 cut(s) 27, 84, 184, 241, 504
LweI GCATC 2 cut(s) 30, 187
MaeI CTAG 1 cut(s) 378
MaeII ACGT 3 cut(s) 269, 284, 355
MaeIII GTNAC 2 cut(s) 143, 244
MalI GATC 1 cut(s) 411
MbiI CCGCTC 2 cut(s) 46, 203
MboI GATC 1 cut(s) 409
MboII GAAGA 5 cut(s) 71, 77, 107, 248, 429
MflI RGATCY 1 cut(s) 409
MhlI GDGCHC 1 cut(s) 377
MluCI AATT 1 cut(s) 510
MlyI GAGTC 4 cut(s) 70, 247, 392, 425
MnlI CCTC 8 cut(s) 42, 91, 93, 114, 199, 270, 300, 495
MroNI GCCGGC 1 cut(s) 490
MspA1I CMGCKG 2 cut(s) 265, 309
MspI CCGG 1 cut(s) 491
MwoI GCNNNNNNNGC 4 cut(s) 232, 271, 286, 487
NaeI GCCGGC 1 cut(s) 492
NcoI CCATGG 1 cut(s) 435
NdeII GATC 1 cut(s) 409
NgoMIV GCCGGC 1 cut(s) 490
NlaIII CATG 2 cut(s) 431, 439
NmuCI GTSAC 2 cut(s) 143, 244
PcsI WCGNNNNNNNCGW 2 cut(s) 132, 278
PdiI GCCGGC 1 cut(s) 492
PflFI GACNNNGTC 1 cut(s) 475
PkrI GCNGC 3 cut(s) 45, 202, 311
PleI GAGTC 4 cut(s) 70, 247, 392, 425
PpsI GAGTC 4 cut(s) 70, 247, 392, 425
Psp124BI GAGCTC 1 cut(s) 377
PspPI GGNCC 1 cut(s) 68
PsuI RGATCY 1 cut(s) 409
PsyI GACNNNGTC 1 cut(s) 475
SacI GAGCTC 1 cut(s) 377
SatI GCNGC 3 cut(s) 44, 201, 310
Sau3AI GATC 1 cut(s) 409
Sau96I GGNCC 1 cut(s) 68
SchI GAGTC 4 cut(s) 70, 247, 392, 425
SduI GDGCHC 1 cut(s) 377
SetI ASST 5 cut(s) 272, 287, 358, 377, 383
SfaNI GCATC 2 cut(s) 30, 187
SfcI CTRYAG 1 cut(s) 297
SmlI CTYRAG 1 cut(s) 461
SmoI CTYRAG 1 cut(s) 461
Sse9I AATT 1 cut(s) 510
SsiI CCGC 5 cut(s) 44, 201, 263, 293, 309
SspI AATATT 1 cut(s) 451
SspMI CTAG 1 cut(s) 378
SstI GAGCTC 1 cut(s) 377
StyI CCWWGG 2 cut(s) 435, 495
TaiI ACGT 3 cut(s) 272, 287, 358
TasI AATT 1 cut(s) 510
TauI GCSGC 3 cut(s) 46, 203, 312
TscAI CASTG 4 cut(s) 25, 42, 152, 182
TseFI GTSAC 2 cut(s) 143, 244
Tsp45I GTSAC 2 cut(s) 143, 244
TspRI CASTG 4 cut(s) 25, 42, 152, 182
Tth111I GACNNNGTC 1 cut(s) 475
XagI CCTNNNNNAGG 1 cut(s) 499
XspI CTAG 1 cut(s) 378
ZraI GACGTC 2 cut(s) 270, 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.