Encoded by
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcuI | CTGAAG | 1 cut(s) | 119 |
| AfaI | GTAC | 1 cut(s) | 13 |
| AgsI | TTSAA | 1 cut(s) | 156 |
| AluBI | AGCT | 2 cut(s) | 139, 159 |
| AluI | AGCT | 2 cut(s) | 139, 159 |
| BbvCI | CCTCAGC | 1 cut(s) | 140 |
| BfaI | CTAG | 1 cut(s) | 24 |
| BmsI | GCATC | 1 cut(s) | 28 |
| Bpu10I | CCTNAGC | 1 cut(s) | 140 |
| BpuEI | CTTGAG | 1 cut(s) | 86 |
| BsaWI | WCCGGW | 1 cut(s) | 94 |
| BseGI | GGATG | 1 cut(s) | 133 |
| BseMII | CTCAG | 1 cut(s) | 131 |
| BsiSI | CCGG | 1 cut(s) | 95 |
| BsmI | GAATGC | 1 cut(s) | 60 |
| BspCNI | CTCAG | 1 cut(s) | 132 |
| BstDEI | CTNAG | 1 cut(s) | 140 |
| BstF5I | GGATG | 1 cut(s) | 133 |
| BtgZI | GCGATG | 1 cut(s) | 51 |
| BtsCI | GGATG | 1 cut(s) | 133 |
| Csp6I | GTAC | 1 cut(s) | 12 |
| CviJI | RGCY | 2 cut(s) | 139, 159 |
| CviKI_1 | RGCY | 2 cut(s) | 139, 159 |
| CviQI | GTAC | 1 cut(s) | 12 |
| DdeI | CTNAG | 1 cut(s) | 140 |
| Eco57I | CTGAAG | 1 cut(s) | 119 |
| FaiI | YATR | 1 cut(s) | 54 |
| FokI | GGATG | 1 cut(s) | 140 |
| FspBI | CTAG | 1 cut(s) | 24 |
| HapII | CCGG | 1 cut(s) | 95 |
| HpaII | CCGG | 1 cut(s) | 95 |
| Hpy188III | TCNNGA | 2 cut(s) | 65, 86 |
| HpyCH4V | TGCA | 1 cut(s) | 41 |
| HpyF3I | CTNAG | 1 cut(s) | 140 |
| LpnPI | CCDG | 1 cut(s) | 108 |
| LweI | GCATC | 1 cut(s) | 28 |
| MaeI | CTAG | 1 cut(s) | 24 |
| MboII | GAAGA | 1 cut(s) | 40 |
| MfeI | CAATTG | 1 cut(s) | 42 |
| MluCI | AATT | 2 cut(s) | 42, 98 |
| MnlI | CCTC | 6 cut(s) | 50, 61, 89, 97, 118, 135 |
| MspA1I | CMGCKG | 1 cut(s) | 139 |
| MspI | CCGG | 1 cut(s) | 95 |
| MunI | CAATTG | 1 cut(s) | 42 |
| Mva1269I | GAATGC | 1 cut(s) | 60 |
| PctI | GAATGC | 1 cut(s) | 60 |
| PvuII | CAGCTG | 1 cut(s) | 139 |
| RsaI | GTAC | 1 cut(s) | 13 |
| RsaNI | GTAC | 1 cut(s) | 12 |
| SetI | ASST | 4 cut(s) | 72, 81, 141, 161 |
| SfaNI | GCATC | 1 cut(s) | 28 |
| SgeI | CNNG | 5 cut(s) | 21, 36, 77, 98, 107 |
| SmlI | CTYRAG | 1 cut(s) | 65 |
| SmoI | CTYRAG | 1 cut(s) | 65 |
| Sse9I | AATT | 2 cut(s) | 42, 98 |
| SspMI | CTAG | 1 cut(s) | 24 |
| TasI | AATT | 2 cut(s) | 42, 98 |
| TspDTI | ATGAA | 1 cut(s) | 144 |
| XspI | CTAG | 1 cut(s) | 24 |