Rmu_sc0004753.1_g000022

EG45-like domain containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004753.1
OG0000421
Physical Location & Seq
Forward (+)
73525 .. 78453
4929 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004753.1_g000022.1.cds

Sequence Viewer

Length: 1506 bp
atggaaagaatgggattcaaaatccgtgttctcatggtggtgatggtggctgtggttatatgtcttccctcagtcacgtttgctgccgagggaactgccaccttctatacacctccctatgttccttcgtcatgctatggataccagaacgatggggtgatgatagctgcagcaagcgatgtcatttggggaaacagagcggcgtgtggaaggaactatacagtgaaatgcattggaggcactaaccaaggtgtaccacaaccttgcaagggtaacagcgttgtcgtaaagattgtcgattattgtcctcctggatgccctggaactatagatctctccaaagaagccttttctgccattgctaatcctgatgctggaaaaatcaatattgagtatactcacttcagcgccgctttggaagttgctcgctcctctgctcctcttcttcgcaagttgcaggtaagatcaatcaagcttctgattgatccgcttcttcaatcaatcaagttttctcctcttcttcgatccgcttggagcttcagtgcttccggaaccatgagtgatggaggaacacctcctattagttctagtgccgggtctgttaatgccggtactagtagtggagctggtgatgctgaaaatgagatcatagatgataaagcaccgttgtggaaatttgtgaagaagattcaaaaacggcaaggtggaggaggttggaagtggcagtgcaatttctgcaaattgtactacagcggctcttatactagagttcgtagtcatttattgaaagaaggaacagcgggggtggcaatttgcaccaaggttactcctcatgcctttactgcaatgaccaagttggcgagagaatccaaggagagattaatgaatgcagctcctagacatgttcctctaccatcatcacaacagcaaggagggagttcctctactagtagctatgggatgagttatcatgcctatacacaagcaccaacagcaagtgatagtggaaagaagaggaaaacagctgctggttcttcttccaggccctactcccactatacagccacaaacagccttagcctgttcagatgcaacaacaccctggagaaaactccttacaaggatcttaaccatgcttgcaagagtgtaaatcatatttactatactgtatcaaattatagtttagagtgcccaaccattcagatacccatacttccagattcccgtcccaatgtctataatcgttctgcattgactgctaaattctcccttcaagtgatatatactaaagaatgtgatcggtgttctagtagaggaggtgaatgcttggatgacaatggagaattgaaatgtgccacgacacaatgctggaattcgaagggagaattcaattgtaccctcgcagaagaaggtaaatgccatgatgagaaggaggaaggcaaatgtgccaaagcaggtaaatgcatcgaaatttgtgtttatactatttgtcttttcatccggtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1

Protein Analysis

501

Amino Acids

54.3

Weight (kDa)

8.99

Isoelectric Point (pI)

46.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000421)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08267
fragaria_vesca FvH4_2g14331 FvH4_2g16371 FvH4_2g26163 FvH4_3g42540 FvH4_4g02102 FvH4_4g12491 FvH4_4g35171 FvH4_5g06632 FvH4_5g06632 FvH4_5g06632 FvH4_5g08811 FvH4_5g08812 FvH4_5g23531 FvH4_6g10272 FvH4_6g40844 FvH4_7g16803 FvH4_7g16804
malus_domestica MD11G1099500.v1.1 MD17G1176800.v1.1
pyrus_communis pycom05g07840 pycom06g08730 pycom07g12010 pycom07g12020 pycom10g07270 pycom11g17470 pycom12g10610 pycom12g14240 pycom13g22040 pycom15g02440
rosa_chinensis RchiOBHm_Chr1g0371321 RchiOBHm_Chr2g0102701 RchiOBHm_Chr3g0465681 RchiOBHm_Chr4g0420941 RchiOBHm_Chr4g0434271 RchiOBHm_Chr4g0442721 RchiOBHm_Chr6g0262081 RchiOBHm_Chr6g0278171 RchiOBHm_Chr6g0294991 RchiOBHm_Chr6g0295001 RchiOBHm_Chr7g0205361 RchiOBHm_Chr7g0205371 RchiOBHm_Chr7g0223471
rosa_laevigata RLG00000005266
rosa_multiflora Rmu_sc0000493.1_g000033 Rmu_sc0000493.1_g000034 Rmu_sc0000507.1_g000007 Rmu_sc0000843.1_g000001 Rmu_sc0001075.1_g000033 Rmu_sc0002077.1_g000007 Rmu_sc0002077.1_g000008 Rmu_sc0002329.1_g000029 Rmu_sc0002539.1_g000077 Rmu_sc0002655.1_g000006 Rmu_sc0003127.1_g000020 Rmu_sc0003765.1_g000054 Rmu_sc0004368.1_g000023 Rmu_sc0004753.1_g000022 Rmu_sc0005297.1_g000014 Rmu_sc0007633.1_g000010 Rmu_sc0008164.1_g000005 Rmu_sc0008191.1_g000003 Rmu_sc0017900.1_g000005 Rmu_sc0022727.1_g000002
rosa_roxburghii Rroxscaffold_2G00079920 Rroxscaffold_3G00255890 Rroxscaffold_4G00295340 Rroxscaffold_4G00296360 Rroxscaffold_4G00299640 Rroxscaffold_6G00388210
rosa_rugosa Rorug02G0466700 Rorug04G0123200 Rorug05G0074800 Rorug07G0202900
rosa_samantha Rh1DG134700 Rh3CG159000 Rh4AG331000 Rh5AG521100 Rh5BG136400 Rh5DG127300 Rh5DG383100 Rh5DG386100 Rh5DG458000 Rh6CG450400 Rh7CG233800 Rh7DG407100
rosa_wichuraiana Rw1G004960 Rw1G005730 Rw1G006360 Rw1G017400 Rw2G013190 Rw2G022210 Rw2G029640 Rw5G020910 Rw5G036930 Rw6G016200 Rw7G018170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 448, 1445
AccBSI CCGCTC 1 cut(s) 200
AccI GTMKAC 1 cut(s) 395
AccIII TCCGGA 1 cut(s) 548
AciI CCGC 6 cut(s) 200, 411, 488, 528, 753, 800
AclWI GGATC 3 cut(s) 479, 519, 1131
AcsI RAATTY 5 cut(s) 674, 1262, 1372, 1385, 1470
AcuI CTGAAG 2 cut(s) 388, 523
AfaI GTAC 4 cut(s) 255, 613, 746, 1396
AfiI CCNNNNNNNGG 2 cut(s) 269, 374
AflIII ACRYGT 1 cut(s) 901
AgsI TTSAA 7 cut(s) 19, 497, 692, 787, 1274, 1348, 1390
AhlI ACTAGT 2 cut(s) 614, 947
AjnI CCWGG 4 cut(s) 310, 319, 1040, 1101
AleI CACNNNNGTG 1 cut(s) 667
AluBI AGCT 7 cut(s) 167, 475, 537, 626, 893, 954, 1025
AluI AGCT 7 cut(s) 167, 475, 537, 626, 893, 954, 1025
AlwI GGATC 3 cut(s) 479, 519, 1131
AlwNI CAGNNNCTG 1 cut(s) 1028
Aor13HI TCCGGA 1 cut(s) 548
AoxI GGCC 1 cut(s) 1043
ApeKI GCWGC 5 cut(s) 83, 167, 170, 890, 1025
ApoI RAATTY 5 cut(s) 674, 1262, 1372, 1385, 1470
AseI ATTAAT 1 cut(s) 881
AspLEI GCGC 1 cut(s) 410
AspS9I GGNCC 1 cut(s) 1044
AsuC2I CCSGG 1 cut(s) 595
AsuHPI GGTGA 4 cut(s) 52, 169, 641, 1331
AsuII TTCGAA 1 cut(s) 1376
BaeGI GKGCMC 1 cut(s) 1193
BarI GAAGNNNNNNTAC 4 cut(s) 202, 234, 386, 418
BbsI GAAGAC 1 cut(s) 56
BbvI GCAGC 5 cut(s) 70, 154, 182, 902, 1012
BccI CCATC 4 cut(s) 37, 146, 557, 922
BceAI ACGGC 1 cut(s) 713
BcgI CGANNNNNNTGC 2 cut(s) 77, 111
BciT130I CCWGG 4 cut(s) 312, 321, 1042, 1103
BciVI GTATCC 1 cut(s) 134
BcnI CCSGG 1 cut(s) 595
BcuI ACTAGT 2 cut(s) 614, 947
BfaI CTAG 6 cut(s) 588, 615, 765, 897, 948, 1308
BfmI CTRYAG 3 cut(s) 168, 327, 748
BfoI RGCGCY 1 cut(s) 411
BfuAI ACCTGC 2 cut(s) 448, 1445
BfuI GTATCC 1 cut(s) 134
BglII AGATCT 1 cut(s) 331
BisI GCNGC 8 cut(s) 84, 168, 171, 201, 411, 754, 891, 1026
BlsI GCNGC 8 cut(s) 85, 169, 172, 202, 412, 755, 892, 1027
Bme1390I CCNGG 5 cut(s) 312, 321, 595, 1042, 1103
BmgT120I GGNCC 1 cut(s) 1044
BmiI GGNNCC 1 cut(s) 553
BmrFI CCNGG 5 cut(s) 312, 321, 595, 1042, 1103
BmsI GCATC 5 cut(s) 305, 361, 622, 1079, 1473
BpiI GAAGAC 1 cut(s) 56
BpmI CTGGAG 1 cut(s) 1124
Bpu10I CCTNAGC 1 cut(s) 1076
Bpu14I TTCGAA 1 cut(s) 1376
BpuMI CCSGG 1 cut(s) 595
BsaJI CCNNGG 6 cut(s) 87, 247, 319, 819, 870, 1101
BsaWI WCCGGW 2 cut(s) 548, 1500
BsaXI ACNNNNNCTCC 2 cut(s) 526, 556
Bsc4I CCNNNNNNNGG 2 cut(s) 269, 374
Bse118I RCCGGY 1 cut(s) 608
Bse3DI GCAATG 2 cut(s) 357, 852
BseAI TCCGGA 1 cut(s) 548
BseBI CCWGG 4 cut(s) 312, 321, 1042, 1103
BseDI CCNNGG 6 cut(s) 87, 247, 319, 819, 870, 1101
BseGI GGATG 4 cut(s) 320, 966, 1336, 1497
BseLI CCNNNNNNNGG 2 cut(s) 269, 374
BseMI GCAATG 2 cut(s) 357, 852
BseMII CTCAG 1 cut(s) 84
BseRI GAGGAG 6 cut(s) 421, 429, 504, 723, 819, 1329
BseSI GKGCMC 1 cut(s) 1193
BseXI GCAGC 5 cut(s) 70, 154, 182, 902, 1012
BshFI GGCC 1 cut(s) 1045
BsiSI CCGG 4 cut(s) 549, 594, 609, 1501
BslFI GGGAC 1 cut(s) 1212
BslI CCNNNNNNNGG 2 cut(s) 269, 374
BsmFI GGGAC 1 cut(s) 1212
BsmI GAATGC 2 cut(s) 892, 1328
BsnI GGCC 1 cut(s) 1045
Bsp119I TTCGAA 1 cut(s) 1376
Bsp1286I GDGCHC 1 cut(s) 1193
Bsp13I TCCGGA 1 cut(s) 548
Bsp143I GATC 7 cut(s) 331, 464, 484, 524, 645, 1123, 1297
BspACI CCGC 6 cut(s) 200, 411, 488, 528, 753, 800
BspANI GGCC 1 cut(s) 1045
BspCNI CTCAG 1 cut(s) 83
BspEI TCCGGA 1 cut(s) 548
BspLI GGNNCC 1 cut(s) 553
BspMAI CTGCAG 1 cut(s) 172
BspMI ACCTGC 2 cut(s) 448, 1445
BspPI GGATC 3 cut(s) 479, 519, 1131
BspT104I TTCGAA 1 cut(s) 1376
BsrBI CCGCTC 1 cut(s) 200
BsrDI GCAATG 2 cut(s) 357, 852
BsrFI RCCGGY 1 cut(s) 608
BssAI RCCGGY 1 cut(s) 608
BssECI CCNNGG 6 cut(s) 87, 247, 319, 819, 870, 1101
BssMI GATC 7 cut(s) 331, 464, 484, 524, 645, 1123, 1297
BssNAI GTATAC 1 cut(s) 396
BssT1I CCWWGG 3 cut(s) 247, 819, 870
Bst1107I GTATAC 1 cut(s) 396
Bst2UI CCWGG 4 cut(s) 312, 321, 1042, 1103
Bst4CI ACNGT 3 cut(s) 223, 666, 1168
Bst6I CTCTTC 3 cut(s) 447, 522, 1007
BstAPI GCANNNNNTGC 2 cut(s) 735, 1256
BstBI TTCGAA 1 cut(s) 1376
BstC8I GCNNGC 3 cut(s) 175, 427, 1138
BstDEI CTNAG 2 cut(s) 70, 1076
BstF5I GGATG 4 cut(s) 320, 966, 1336, 1497
BstH2I RGCGCY 1 cut(s) 411
BstHHI GCGC 1 cut(s) 410
BstKTI GATC 7 cut(s) 334, 467, 487, 527, 648, 1126, 1300
BstMBI GATC 7 cut(s) 331, 464, 484, 524, 645, 1123, 1297
BstMWI GCNNNNNNNGC 8 cut(s) 237, 353, 632, 735, 806, 842, 992, 1256
BstNI CCWGG 4 cut(s) 312, 321, 1042, 1103
BstNSI RCATGY 1 cut(s) 905
BstSCI CCNGG 5 cut(s) 310, 319, 593, 1040, 1101
BstSFI CTRYAG 3 cut(s) 168, 327, 748
BstSLI GKGCMC 1 cut(s) 1193
BstV1I GCAGC 5 cut(s) 70, 154, 182, 902, 1012
BstV2I GAAGAC 1 cut(s) 56
BstX2I RGATCY 2 cut(s) 331, 1123
BstXI CCANNNNNNTGG 1 cut(s) 152
BstYI RGATCY 2 cut(s) 331, 1123
BstZ17I GTATAC 1 cut(s) 396
BsuI GTATCC 1 cut(s) 134
BsuRI GGCC 1 cut(s) 1045
BtgZI GCGATG 1 cut(s) 192
BtsCI GGATG 4 cut(s) 320, 966, 1336, 1497
BtsI GCAGTG 1 cut(s) 731
BtsIMutI CAGTG 3 cut(s) 228, 547, 731
BveI ACCTGC 2 cut(s) 448, 1445
Cac8I GCNNGC 3 cut(s) 175, 427, 1138
CaiI CAGNNNCTG 1 cut(s) 1028
CfoI GCGC 1 cut(s) 410
Cfr10I RCCGGY 1 cut(s) 608
Cfr13I GGNCC 1 cut(s) 1044
Csp6I GTAC 4 cut(s) 254, 612, 745, 1395
CviAII CATG 8 cut(s) 34, 132, 556, 833, 902, 971, 1133, 1421
CviQI GTAC 4 cut(s) 254, 612, 745, 1395
DdeI CTNAG 2 cut(s) 70, 1076
DpnI GATC 7 cut(s) 333, 466, 486, 526, 647, 1125, 1299
DpnII GATC 7 cut(s) 331, 464, 484, 524, 645, 1123, 1297
Eam1104I CTCTTC 3 cut(s) 447, 522, 1007
EarI CTCTTC 3 cut(s) 447, 522, 1007
Eco130I CCWWGG 3 cut(s) 247, 819, 870
Eco57I CTGAAG 2 cut(s) 388, 523
EcoO109I RGGNCCY 1 cut(s) 1044
EcoRI GAATTC 2 cut(s) 1372, 1385
EcoRII CCWGG 4 cut(s) 310, 319, 1040, 1101
EcoT14I CCWWGG 3 cut(s) 247, 819, 870
EcoT22I ATGCAT 2 cut(s) 233, 1466
ErhI CCWWGG 3 cut(s) 247, 819, 870
FaeI CATG 8 cut(s) 37, 135, 559, 836, 905, 974, 1136, 1424
FaqI GGGAC 1 cut(s) 1212
FatI CATG 8 cut(s) 33, 131, 555, 832, 901, 970, 1132, 1420
FauI CCCGC 1 cut(s) 793
FblI GTMKAC 1 cut(s) 395
Fnu4HI GCNGC 8 cut(s) 84, 168, 171, 201, 411, 754, 891, 1026
FokI GGATG 4 cut(s) 327, 973, 1343, 1484
Fsp4HI GCNGC 8 cut(s) 84, 168, 171, 201, 411, 754, 891, 1026
FspBI CTAG 6 cut(s) 588, 615, 765, 897, 948, 1308
GlaI GCGC 1 cut(s) 409
GluI GCNGC 8 cut(s) 84, 168, 171, 201, 411, 754, 891, 1026
GsuI CTGGAG 1 cut(s) 1124
HaeII RGCGCY 1 cut(s) 411
HaeIII GGCC 1 cut(s) 1045
HapII CCGG 4 cut(s) 549, 594, 609, 1501
HhaI GCGC 1 cut(s) 410
Hin1II CATG 8 cut(s) 37, 135, 559, 836, 905, 974, 1136, 1424
Hin6I GCGC 1 cut(s) 408
HinP1I GCGC 1 cut(s) 408
HindIII AAGCTT 1 cut(s) 473
HinfI GANTC 4 cut(s) 15, 688, 866, 1220
HpaII CCGG 4 cut(s) 549, 594, 609, 1501
HphI GGTGA 4 cut(s) 52, 169, 641, 1331
Hpy166II GTNNAC 2 cut(s) 254, 396
Hpy188I TCNGA 3 cut(s) 480, 1088, 1203
Hpy188III TCNNGA 3 cut(s) 368, 549, 1217
Hpy8I GTNNAC 2 cut(s) 254, 396
HpyAV CCTTC 9 cut(s) 112, 135, 204, 785, 1280, 1372, 1403, 1423, 1430
HpyCH4III ACNGT 3 cut(s) 223, 666, 1168
HpyCH4IV ACGT 1 cut(s) 77
HpyF10VI GCNNNNNNNGC 8 cut(s) 237, 353, 632, 735, 806, 842, 992, 1256
HpyF3I CTNAG 2 cut(s) 70, 1076
HpySE526I ACGT 1 cut(s) 77
Hsp92II CATG 8 cut(s) 37, 135, 559, 836, 905, 974, 1136, 1424
HspAI GCGC 1 cut(s) 408
Kpn2I TCCGGA 1 cut(s) 548
Kzo9I GATC 7 cut(s) 331, 464, 484, 524, 645, 1123, 1297
LmnI GCTCC 5 cut(s) 434, 442, 534, 623, 898
Lsp1109I GCAGC 5 cut(s) 70, 154, 182, 902, 1012
LweI GCATC 5 cut(s) 305, 361, 622, 1079, 1473
MaeI CTAG 6 cut(s) 588, 615, 765, 897, 948, 1308
MaeII ACGT 1 cut(s) 77
MaeIII GTNAC 3 cut(s) 73, 272, 823
MalI GATC 7 cut(s) 333, 466, 486, 526, 647, 1125, 1299
MbiI CCGCTC 1 cut(s) 200
MboI GATC 7 cut(s) 331, 464, 484, 524, 645, 1123, 1297
MfeI CAATTG 1 cut(s) 1390
MflI RGATCY 2 cut(s) 331, 1123
MhlI GDGCHC 1 cut(s) 1193
MmeI TCCRAC 1 cut(s) 695
Mph1103I ATGCAT 2 cut(s) 233, 1466
MroI TCCGGA 1 cut(s) 548
MseI TTAA 3 cut(s) 603, 881, 1128
MslI CAYNNNNRTG 2 cut(s) 38, 667
MspA1I CMGCKG 3 cut(s) 753, 800, 1025
MspI CCGG 4 cut(s) 549, 594, 609, 1501
MspR9I CCNGG 5 cut(s) 312, 321, 595, 1042, 1103
MunI CAATTG 1 cut(s) 1390
Mva1269I GAATGC 2 cut(s) 892, 1328
MvaI CCWGG 4 cut(s) 312, 321, 1042, 1103
MwoI GCNNNNNNNGC 8 cut(s) 237, 353, 632, 735, 806, 842, 992, 1256
NciI CCSGG 1 cut(s) 595
NdeII GATC 7 cut(s) 331, 464, 484, 524, 645, 1123, 1297
NlaIII CATG 8 cut(s) 37, 135, 559, 836, 905, 974, 1136, 1424
NlaIV GGNNCC 1 cut(s) 553
NmeAIII GCCGAG 1 cut(s) 112
NmuCI GTSAC 1 cut(s) 73
NsiI ATGCAT 2 cut(s) 233, 1466
NspI RCATGY 1 cut(s) 905
NspV TTCGAA 1 cut(s) 1376
OliI CACNNNNGTG 1 cut(s) 667
PciI ACATGT 1 cut(s) 901
PctI GAATGC 2 cut(s) 892, 1328
PfeI GAWTC 4 cut(s) 15, 688, 866, 1220
PfoI TCCNGGA 1 cut(s) 310
PkrI GCNGC 8 cut(s) 85, 169, 172, 202, 412, 755, 892, 1027
PscI ACATGT 1 cut(s) 901
PshBI ATTAAT 1 cut(s) 881
Psp6I CCWGG 4 cut(s) 310, 319, 1040, 1101
PspGI CCWGG 4 cut(s) 310, 319, 1040, 1101
PspN4I GGNNCC 1 cut(s) 553
PspPI GGNCC 1 cut(s) 1044
PstI CTGCAG 1 cut(s) 172
PstNI CAGNNNCTG 1 cut(s) 1028
PsuI RGATCY 2 cut(s) 331, 1123
PvuII CAGCTG 1 cut(s) 1025
RsaI GTAC 4 cut(s) 255, 613, 746, 1396
RsaNI GTAC 4 cut(s) 254, 612, 745, 1395
RseI CAYNNNNRTG 2 cut(s) 38, 667
SaqAI TTAA 3 cut(s) 603, 881, 1128
SatI GCNGC 8 cut(s) 84, 168, 171, 201, 411, 754, 891, 1026
Sau3AI GATC 7 cut(s) 331, 464, 484, 524, 645, 1123, 1297
Sau96I GGNCC 1 cut(s) 1044
ScrFI CCNGG 5 cut(s) 312, 321, 595, 1042, 1103
SduI GDGCHC 1 cut(s) 1193
SfaNI GCATC 5 cut(s) 305, 361, 622, 1079, 1473
SfcI CTRYAG 3 cut(s) 168, 327, 748
SfuI TTCGAA 1 cut(s) 1376
SmiMI CAYNNNNRTG 2 cut(s) 38, 667
SpeI ACTAGT 2 cut(s) 614, 947
SsiI CCGC 6 cut(s) 200, 411, 488, 528, 753, 800
SspI AATATT 1 cut(s) 388
SspMI CTAG 6 cut(s) 588, 615, 765, 897, 948, 1308
StyD4I CCNGG 5 cut(s) 310, 319, 593, 1040, 1101
StyI CCWWGG 3 cut(s) 247, 819, 870
TaaI ACNGT 3 cut(s) 223, 666, 1168
TaiI ACGT 1 cut(s) 80
TaqI TCGA 4 cut(s) 297, 523, 1376, 1467
TatI WGTACW 1 cut(s) 744
TauI GCSGC 3 cut(s) 203, 413, 756
TfiI GAWTC 4 cut(s) 15, 688, 866, 1220
Tru1I TTAA 3 cut(s) 603, 881, 1128
Tru9I TTAA 3 cut(s) 603, 881, 1128
TscAI CASTG 3 cut(s) 228, 547, 731
TseFI GTSAC 1 cut(s) 73
TseI GCWGC 5 cut(s) 83, 167, 170, 890, 1025
Tsp45I GTSAC 1 cut(s) 73
TspDTI ATGAA 2 cut(s) 899, 1486
TspGWI ACGGA 1 cut(s) 14
TspRI CASTG 3 cut(s) 228, 547, 731
VspI ATTAAT 1 cut(s) 881
XapI RAATTY 5 cut(s) 674, 1262, 1372, 1385, 1470
XceI RCATGY 1 cut(s) 905
XmiI GTMKAC 1 cut(s) 395
XspI CTAG 6 cut(s) 588, 615, 765, 897, 948, 1308
Zsp2I ATGCAT 2 cut(s) 233, 1466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.