Encoded by
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 251 |
| AcsI | RAATTY | 1 cut(s) | 98 |
| AcuI | CTGAAG | 1 cut(s) | 210 |
| AfaI | GTAC | 1 cut(s) | 148 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 128 |
| AgsI | TTSAA | 4 cut(s) | 119, 154, 231, 274 |
| Alw26I | GTCTC | 2 cut(s) | 199, 228 |
| ApoI | RAATTY | 1 cut(s) | 98 |
| AspS9I | GGNCC | 1 cut(s) | 109 |
| AsuHPI | GGTGA | 2 cut(s) | 59, 143 |
| AvaII | GGWCC | 1 cut(s) | 109 |
| BcoDI | GTCTC | 2 cut(s) | 199, 228 |
| Bme18I | GGWCC | 1 cut(s) | 109 |
| BmgT120I | GGNCC | 1 cut(s) | 109 |
| BmrI | ACTGGG | 1 cut(s) | 48 |
| BmuI | ACTGGG | 1 cut(s) | 48 |
| BpuEI | CTTGAG | 1 cut(s) | 203 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 128 |
| Bse1I | ACTGG | 1 cut(s) | 54 |
| Bse3DI | GCAATG | 1 cut(s) | 175 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 128 |
| BseMI | GCAATG | 1 cut(s) | 175 |
| BseMII | CTCAG | 1 cut(s) | 210 |
| BseNI | ACTGG | 1 cut(s) | 54 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 128 |
| BsmAI | GTCTC | 2 cut(s) | 199, 228 |
| Bsp1407I | TGTACA | 1 cut(s) | 146 |
| BspACI | CCGC | 1 cut(s) | 251 |
| BspCNI | CTCAG | 1 cut(s) | 209 |
| BsrDI | GCAATG | 1 cut(s) | 175 |
| BsrGI | TGTACA | 1 cut(s) | 146 |
| BsrI | ACTGG | 1 cut(s) | 54 |
| Bst4CI | ACNGT | 1 cut(s) | 109 |
| BstAUI | TGTACA | 1 cut(s) | 146 |
| BstDEI | CTNAG | 2 cut(s) | 123, 196 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 126 |
| BstMAI | GTCTC | 2 cut(s) | 199, 228 |
| BtgZI | GCGATG | 1 cut(s) | 21 |
| BtsIMutI | CAGTG | 1 cut(s) | 61 |
| Cfr13I | GGNCC | 1 cut(s) | 109 |
| Csp6I | GTAC | 1 cut(s) | 147 |
| CviQI | GTAC | 1 cut(s) | 147 |
| DdeI | CTNAG | 2 cut(s) | 123, 196 |
| Eco47I | GGWCC | 1 cut(s) | 109 |
| Eco57I | CTGAAG | 1 cut(s) | 210 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 126 |
| FauI | CCCGC | 1 cut(s) | 258 |
| HinfI | GANTC | 1 cut(s) | 29 |
| HphI | GGTGA | 2 cut(s) | 59, 143 |
| Hpy166II | GTNNAC | 1 cut(s) | 50 |
| Hpy188I | TCNGA | 1 cut(s) | 320 |
| Hpy188III | TCNNGA | 1 cut(s) | 302 |
| Hpy8I | GTNNAC | 1 cut(s) | 50 |
| Hpy99I | CGWCG | 1 cut(s) | 284 |
| HpyAV | CCTTC | 3 cut(s) | 128, 232, 237 |
| HpyCH4III | ACNGT | 1 cut(s) | 109 |
| HpyCH4V | TGCA | 2 cut(s) | 11, 168 |
| HpyF3I | CTNAG | 2 cut(s) | 123, 196 |
| LpnPI | CCDG | 1 cut(s) | 67 |
| MaeIII | GTNAC | 2 cut(s) | 16, 103 |
| MfeI | CAATTG | 1 cut(s) | 142 |
| MluCI | AATT | 3 cut(s) | 98, 142, 177 |
| MnlI | CCTC | 4 cut(s) | 37, 131, 265, 270 |
| MslI | CAYNNNNRTG | 1 cut(s) | 154 |
| MunI | CAATTG | 1 cut(s) | 142 |
| PfeI | GAWTC | 1 cut(s) | 29 |
| PspPI | GGNCC | 1 cut(s) | 109 |
| RsaI | GTAC | 1 cut(s) | 148 |
| RsaNI | GTAC | 1 cut(s) | 147 |
| RseI | CAYNNNNRTG | 1 cut(s) | 154 |
| Sau96I | GGNCC | 1 cut(s) | 109 |
| SetI | ASST | 2 cut(s) | 124, 133 |
| SgeI | CNNG | 7 cut(s) | 66, 125, 166, 232, 262, 278, 314 |
| SinI | GGWCC | 1 cut(s) | 109 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 154 |
| SmlI | CTYRAG | 1 cut(s) | 218 |
| SmoI | CTYRAG | 1 cut(s) | 218 |
| Sse9I | AATT | 3 cut(s) | 98, 142, 177 |
| SsiI | CCGC | 1 cut(s) | 251 |
| TaaI | ACNGT | 1 cut(s) | 109 |
| TaqI | TCGA | 1 cut(s) | 279 |
| TasI | AATT | 3 cut(s) | 98, 142, 177 |
| TatI | WGTACW | 1 cut(s) | 146 |
| TfiI | GAWTC | 1 cut(s) | 29 |
| TscAI | CASTG | 1 cut(s) | 61 |
| TspDTI | ATGAA | 2 cut(s) | 21, 251 |
| TspRI | CASTG | 1 cut(s) | 61 |
| VpaK11BI | GGWCC | 1 cut(s) | 109 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 126 |
| XapI | RAATTY | 1 cut(s) | 98 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 61 |