Encoded by
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 585, 633 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 611 |
| AciI | CCGC | 2 cut(s) | 464, 1488 |
| AclWI | GGATC | 2 cut(s) | 211, 1867 |
| AcoI | YGGCCR | 1 cut(s) | 620 |
| AcsI | RAATTY | 4 cut(s) | 116, 1123, 1532, 1790 |
| AcuI | CTGAAG | 1 cut(s) | 2019 |
| AfaI | GTAC | 5 cut(s) | 166, 193, 397, 1550, 1867 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 146, 611 |
| AflII | CTTAAG | 2 cut(s) | 1229, 1712 |
| AflIII | ACRYGT | 1 cut(s) | 901 |
| AgeI | ACCGGT | 2 cut(s) | 323, 533 |
| AhlI | ACTAGT | 1 cut(s) | 349 |
| AjnI | CCWGG | 2 cut(s) | 610, 909 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 1097, 1129, 1564, 1596 |
| Alw21I | GWGCWC | 3 cut(s) | 1170, 1654, 1946 |
| Alw26I | GTCTC | 1 cut(s) | 314 |
| AlwI | GGATC | 2 cut(s) | 211, 1867 |
| AoxI | GGCC | 3 cut(s) | 620, 1323, 1485 |
| ApeKI | GCWGC | 3 cut(s) | 593, 785, 944 |
| ApoI | RAATTY | 4 cut(s) | 116, 1123, 1532, 1790 |
| AseI | ATTAAT | 1 cut(s) | 960 |
| AsiGI | ACCGGT | 2 cut(s) | 323, 533 |
| AspS9I | GGNCC | 7 cut(s) | 127, 461, 747, 800, 893, 1323, 1572 |
| AsuC2I | CCSGG | 1 cut(s) | 275 |
| AsuHPI | GGTGA | 5 cut(s) | 71, 77, 161, 1282, 1578 |
| AsuII | TTCGAA | 1 cut(s) | 839 |
| AsuNHI | GCTAGC | 2 cut(s) | 929, 1561 |
| AvaII | GGWCC | 6 cut(s) | 127, 461, 747, 800, 893, 1572 |
| BanII | GRGCYC | 1 cut(s) | 1654 |
| BbsI | GAAGAC | 3 cut(s) | 769, 1430, 1655 |
| Bbv12I | GWGCWC | 3 cut(s) | 1170, 1654, 1946 |
| BbvCI | CCTCAGC | 1 cut(s) | 1101 |
| BbvI | GCAGC | 3 cut(s) | 580, 797, 931 |
| BccI | CCATC | 4 cut(s) | 734, 1261, 1403, 1563 |
| BceAI | ACGGC | 1 cut(s) | 1310 |
| BcgI | CGANNNNNNTGC | 1 cut(s) | 30 |
| BciT130I | CCWGG | 2 cut(s) | 612, 911 |
| BciVI | GTATCC | 1 cut(s) | 2101 |
| BclI | TGATCA | 1 cut(s) | 1279 |
| BcnI | CCSGG | 1 cut(s) | 275 |
| BcoDI | GTCTC | 1 cut(s) | 314 |
| BcuI | ACTAGT | 1 cut(s) | 349 |
| BfrI | CTTAAG | 2 cut(s) | 1229, 1712 |
| BfuI | GTATCC | 1 cut(s) | 2101 |
| BisI | GCNGC | 4 cut(s) | 594, 786, 945, 1488 |
| BlsI | GCNGC | 4 cut(s) | 595, 787, 946, 1489 |
| Bme1390I | CCNGG | 3 cut(s) | 275, 612, 911 |
| Bme18I | GGWCC | 6 cut(s) | 127, 461, 747, 800, 893, 1572 |
| BmgT120I | GGNCC | 7 cut(s) | 127, 461, 747, 800, 893, 1323, 1572 |
| BmiI | GGNNCC | 2 cut(s) | 435, 693 |
| BmrFI | CCNGG | 3 cut(s) | 275, 612, 911 |
| BmrI | ACTGGG | 1 cut(s) | 66 |
| BmsI | GCATC | 8 cut(s) | 235, 660, 1063, 1215, 1342, 1395, 1453, 1818 |
| BmtI | GCTAGC | 2 cut(s) | 933, 1565 |
| BmuI | ACTGGG | 1 cut(s) | 66 |
| BpiI | GAAGAC | 3 cut(s) | 769, 1430, 1655 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 634, 666, 1751, 1783 |
| BpmI | CTGGAG | 1 cut(s) | 969 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1101, 1409 |
| Bpu14I | TTCGAA | 1 cut(s) | 839 |
| BpuEI | CTTGAG | 2 cut(s) | 218, 2150 |
| BpuMI | CCSGG | 1 cut(s) | 275 |
| BsaI | GGTCTC | 1 cut(s) | 314 |
| BsaJI | CCNNGG | 2 cut(s) | 1138, 1855 |
| BsaWI | WCCGGW | 3 cut(s) | 323, 533, 2158 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1860, 1890 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 146, 611 |
| Bse118I | RCCGGY | 2 cut(s) | 323, 533 |
| Bse1I | ACTGG | 1 cut(s) | 72 |
| Bse3DI | GCAATG | 3 cut(s) | 193, 1191, 1594 |
| BseBI | CCWGG | 2 cut(s) | 612, 911 |
| BseDI | CCNNGG | 2 cut(s) | 1138, 1855 |
| BseGI | GGATG | 8 cut(s) | 424, 675, 1111, 1272, 1468, 1792, 2083, 2095 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 146, 611 |
| BseMI | GCAATG | 3 cut(s) | 193, 1191, 1594 |
| BseMII | CTCAG | 1 cut(s) | 1092 |
| BseNI | ACTGG | 1 cut(s) | 72 |
| BseRI | GAGGAG | 3 cut(s) | 1429, 1772, 1984 |
| BseXI | GCAGC | 3 cut(s) | 580, 797, 931 |
| BshFI | GGCC | 3 cut(s) | 622, 1325, 1487 |
| BshTI | ACCGGT | 2 cut(s) | 323, 533 |
| BsiHKAI | GWGCWC | 3 cut(s) | 1170, 1654, 1946 |
| BsiSI | CCGG | 4 cut(s) | 275, 324, 534, 2159 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 146, 611 |
| BsmAI | GTCTC | 1 cut(s) | 314 |
| BsmI | GAATGC | 1 cut(s) | 2124 |
| BsnI | GGCC | 3 cut(s) | 622, 1325, 1487 |
| Bso31I | GGTCTC | 1 cut(s) | 314 |
| Bsp119I | TTCGAA | 1 cut(s) | 839 |
| Bsp1286I | GDGCHC | 3 cut(s) | 1170, 1654, 1946 |
| Bsp1407I | TGTACA | 2 cut(s) | 164, 1548 |
| Bsp143I | GATC | 4 cut(s) | 203, 1245, 1279, 1859 |
| Bsp19I | CCATGG | 2 cut(s) | 1138, 1855 |
| BspACI | CCGC | 2 cut(s) | 464, 1488 |
| BspANI | GGCC | 3 cut(s) | 622, 1325, 1487 |
| BspCNI | CTCAG | 1 cut(s) | 1093 |
| BspLI | GGNNCC | 2 cut(s) | 435, 693 |
| BspOI | GCTAGC | 2 cut(s) | 933, 1565 |
| BspPI | GGATC | 2 cut(s) | 211, 1867 |
| BspQI | GCTCTTC | 1 cut(s) | 1515 |
| BspT104I | TTCGAA | 1 cut(s) | 839 |
| BspTI | CTTAAG | 2 cut(s) | 1229, 1712 |
| BspTNI | GGTCTC | 1 cut(s) | 314 |
| BsrDI | GCAATG | 3 cut(s) | 193, 1191, 1594 |
| BsrFI | RCCGGY | 2 cut(s) | 323, 533 |
| BsrGI | TGTACA | 2 cut(s) | 164, 1548 |
| BsrI | ACTGG | 1 cut(s) | 72 |
| BssAI | RCCGGY | 2 cut(s) | 323, 533 |
| BssECI | CCNNGG | 2 cut(s) | 1138, 1855 |
| BssMI | GATC | 4 cut(s) | 203, 1245, 1279, 1859 |
| BssT1I | CCWWGG | 2 cut(s) | 1138, 1855 |
| Bst2UI | CCWGG | 2 cut(s) | 612, 911 |
| Bst4CI | ACNGT | 3 cut(s) | 127, 827, 2113 |
| Bst6I | CTCTTC | 6 cut(s) | 437, 657, 1515, 1596, 1728, 1924 |
| BstAFI | CTTAAG | 2 cut(s) | 1229, 1712 |
| BstAUI | TGTACA | 2 cut(s) | 164, 1548 |
| BstBI | TTCGAA | 1 cut(s) | 839 |
| BstC8I | GCNNGC | 3 cut(s) | 931, 1563, 1780 |
| BstDEI | CTNAG | 4 cut(s) | 141, 1101, 1409, 1692 |
| BstDSI | CCRYGG | 2 cut(s) | 1138, 1855 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 144 |
| BstF5I | GGATG | 8 cut(s) | 424, 675, 1111, 1272, 1468, 1792, 2083, 2095 |
| BstKTI | GATC | 4 cut(s) | 206, 1248, 1282, 1862 |
| BstMAI | GTCTC | 1 cut(s) | 314 |
| BstMBI | GATC | 4 cut(s) | 203, 1245, 1279, 1859 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 950, 1073 |
| BstNI | CCWGG | 2 cut(s) | 612, 911 |
| BstNSI | RCATGY | 3 cut(s) | 905, 1080, 1782 |
| BstSCI | CCNGG | 3 cut(s) | 273, 610, 909 |
| BstV1I | GCAGC | 3 cut(s) | 580, 797, 931 |
| BstV2I | GAAGAC | 3 cut(s) | 769, 1430, 1655 |
| BstX2I | RGATCY | 1 cut(s) | 1859 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1842 |
| BstYI | RGATCY | 1 cut(s) | 1859 |
| BsuI | GTATCC | 1 cut(s) | 2101 |
| BsuRI | GGCC | 3 cut(s) | 622, 1325, 1487 |
| BtgI | CCRYGG | 2 cut(s) | 1138, 1855 |
| BtgZI | GCGATG | 1 cut(s) | 21 |
| BtsCI | GGATG | 8 cut(s) | 424, 675, 1111, 1272, 1468, 1792, 2083, 2095 |
| BtsI | GCAGTG | 1 cut(s) | 825 |
| BtsIMutI | CAGTG | 3 cut(s) | 79, 825, 918 |
| Cac8I | GCNNGC | 3 cut(s) | 931, 1563, 1780 |
| Cfr10I | RCCGGY | 2 cut(s) | 323, 533 |
| Cfr13I | GGNCC | 7 cut(s) | 127, 461, 747, 800, 893, 1323, 1572 |
| CseI | GACGC | 1 cut(s) | 1200 |
| Csp6I | GTAC | 5 cut(s) | 165, 192, 396, 1549, 1866 |
| CspAI | ACCGGT | 2 cut(s) | 323, 533 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 223, 258 |
| CviQI | GTAC | 5 cut(s) | 165, 192, 396, 1549, 1866 |
| DdeI | CTNAG | 4 cut(s) | 141, 1101, 1409, 1692 |
| DpnI | GATC | 4 cut(s) | 205, 1247, 1281, 1861 |
| DpnII | GATC | 4 cut(s) | 203, 1245, 1279, 1859 |
| DraI | TTTAAA | 1 cut(s) | 1893 |
| EaeI | YGGCCR | 1 cut(s) | 620 |
| Eam1104I | CTCTTC | 6 cut(s) | 437, 657, 1515, 1596, 1728, 1924 |
| EarI | CTCTTC | 6 cut(s) | 437, 657, 1515, 1596, 1728, 1924 |
| Ecl136II | GAGCTC | 1 cut(s) | 1652 |
| Eco130I | CCWWGG | 2 cut(s) | 1138, 1855 |
| Eco24I | GRGCYC | 1 cut(s) | 1654 |
| Eco31I | GGTCTC | 1 cut(s) | 314 |
| Eco47I | GGWCC | 6 cut(s) | 127, 461, 747, 800, 893, 1572 |
| Eco53kI | GAGCTC | 1 cut(s) | 1652 |
| Eco57I | CTGAAG | 1 cut(s) | 2019 |
| EcoICRI | GAGCTC | 1 cut(s) | 1652 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 144 |
| EcoRII | CCWGG | 2 cut(s) | 610, 909 |
| EcoT14I | CCWWGG | 2 cut(s) | 1138, 1855 |
| EcoT22I | ATGCAT | 1 cut(s) | 1078 |
| EcoT38I | GRGCYC | 1 cut(s) | 1654 |
| ErhI | CCWWGG | 2 cut(s) | 1138, 1855 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1695, 1727, 1873, 1905 |
| FauNDI | CATATG | 2 cut(s) | 400, 1031 |
| FbaI | TGATCA | 1 cut(s) | 1279 |
| Fnu4HI | GCNGC | 4 cut(s) | 594, 786, 945, 1488 |
| FokI | GGATG | 8 cut(s) | 431, 682, 1118, 1279, 1475, 1799, 2090, 2102 |
| FriOI | GRGCYC | 1 cut(s) | 1654 |
| Fsp4HI | GCNGC | 4 cut(s) | 594, 786, 945, 1488 |
| GluI | GCNGC | 4 cut(s) | 594, 786, 945, 1488 |
| GsuI | CTGGAG | 1 cut(s) | 969 |
| HaeIII | GGCC | 3 cut(s) | 622, 1325, 1487 |
| HapII | CCGG | 4 cut(s) | 275, 324, 534, 2159 |
| HgaI | GACGC | 1 cut(s) | 1200 |
| HindIII | AAGCTT | 2 cut(s) | 312, 1290 |
| HinfI | GANTC | 9 cut(s) | 41, 562, 752, 794, 836, 988, 1615, 1805, 2099 |
| HpaII | CCGG | 4 cut(s) | 275, 324, 534, 2159 |
| HphI | GGTGA | 5 cut(s) | 71, 77, 161, 1282, 1578 |
| Hpy166II | GTNNAC | 7 cut(s) | 68, 461, 823, 896, 1549, 1551, 1868 |
| Hpy188I | TCNGA | 4 cut(s) | 751, 862, 1531, 1644 |
| Hpy188III | TCNNGA | 6 cut(s) | 235, 798, 1259, 1676, 2129, 2150 |
| Hpy8I | GTNNAC | 7 cut(s) | 68, 461, 823, 896, 1549, 1551, 1868 |
| HpyCH4III | ACNGT | 3 cut(s) | 127, 827, 2113 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 950, 1073 |
| HpyF3I | CTNAG | 4 cut(s) | 141, 1101, 1409, 1692 |
| Ksp22I | TGATCA | 1 cut(s) | 1279 |
| Kzo9I | GATC | 4 cut(s) | 203, 1245, 1279, 1859 |
| LguI | GCTCTTC | 1 cut(s) | 1515 |
| LmnI | GCTCC | 4 cut(s) | 950, 1649, 1657, 1941 |
| Lsp1109I | GCAGC | 3 cut(s) | 580, 797, 931 |
| LweI | GCATC | 8 cut(s) | 235, 660, 1063, 1215, 1342, 1395, 1453, 1818 |
| MaeIII | GTNAC | 2 cut(s) | 121, 1309 |
| MalI | GATC | 4 cut(s) | 205, 1247, 1281, 1861 |
| MboI | GATC | 4 cut(s) | 203, 1245, 1279, 1859 |
| MfeI | CAATTG | 3 cut(s) | 160, 500, 934 |
| MflI | RGATCY | 1 cut(s) | 1859 |
| MhlI | GDGCHC | 3 cut(s) | 1170, 1654, 1946 |
| MlyI | GAGTC | 2 cut(s) | 982, 2093 |
| MmeI | TCCRAC | 2 cut(s) | 669, 2062 |
| Mph1103I | ATGCAT | 1 cut(s) | 1078 |
| MseI | TTAA | 8 cut(s) | 413, 699, 960, 1161, 1230, 1713, 1892, 2115 |
| MslI | CAYNNNNRTG | 2 cut(s) | 172, 1220 |
| MspCI | CTTAAG | 2 cut(s) | 1229, 1712 |
| MspI | CCGG | 4 cut(s) | 275, 324, 534, 2159 |
| MspR9I | CCNGG | 3 cut(s) | 275, 612, 911 |
| MunI | CAATTG | 3 cut(s) | 160, 500, 934 |
| Mva1269I | GAATGC | 1 cut(s) | 2124 |
| MvaI | CCWGG | 2 cut(s) | 612, 911 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 950, 1073 |
| NciI | CCSGG | 1 cut(s) | 275 |
| NcoI | CCATGG | 2 cut(s) | 1138, 1855 |
| NdeI | CATATG | 2 cut(s) | 400, 1031 |
| NdeII | GATC | 4 cut(s) | 203, 1245, 1279, 1859 |
| NheI | GCTAGC | 2 cut(s) | 929, 1561 |
| NlaIV | GGNNCC | 2 cut(s) | 435, 693 |
| NmuCI | GTSAC | 1 cut(s) | 1309 |
| NsiI | ATGCAT | 1 cut(s) | 1078 |
| NspI | RCATGY | 3 cut(s) | 905, 1080, 1782 |
| NspV | TTCGAA | 1 cut(s) | 839 |
| PaeI | GCATGC | 1 cut(s) | 1782 |
| PciI | ACATGT | 1 cut(s) | 901 |
| PciSI | GCTCTTC | 1 cut(s) | 1515 |
| PctI | GAATGC | 1 cut(s) | 2124 |
| PfeI | GAWTC | 7 cut(s) | 41, 562, 752, 794, 836, 1615, 1805 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 611 |
| PfoI | TCCNGGA | 1 cut(s) | 273 |
| PinAI | ACCGGT | 2 cut(s) | 323, 533 |
| PkrI | GCNGC | 4 cut(s) | 595, 787, 946, 1489 |
| PleI | GAGTC | 2 cut(s) | 982, 2093 |
| PpsI | GAGTC | 2 cut(s) | 982, 2093 |
| PscI | ACATGT | 1 cut(s) | 901 |
| PshBI | ATTAAT | 1 cut(s) | 960 |
| PsiI | TTATAA | 2 cut(s) | 585, 633 |
| Psp124BI | GAGCTC | 1 cut(s) | 1654 |
| Psp6I | CCWGG | 2 cut(s) | 610, 909 |
| PspGI | CCWGG | 2 cut(s) | 610, 909 |
| PspN4I | GGNNCC | 2 cut(s) | 435, 693 |
| PspPI | GGNCC | 7 cut(s) | 127, 461, 747, 800, 893, 1323, 1572 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 417, 449 |
| PsuI | RGATCY | 1 cut(s) | 1859 |
| RsaI | GTAC | 5 cut(s) | 166, 193, 397, 1550, 1867 |
| RsaNI | GTAC | 5 cut(s) | 165, 192, 396, 1549, 1866 |
| RseI | CAYNNNNRTG | 2 cut(s) | 172, 1220 |
| SacI | GAGCTC | 1 cut(s) | 1654 |
| SapI | GCTCTTC | 1 cut(s) | 1515 |
| SaqAI | TTAA | 8 cut(s) | 413, 699, 960, 1161, 1230, 1713, 1892, 2115 |
| SatI | GCNGC | 4 cut(s) | 594, 786, 945, 1488 |
| Sau3AI | GATC | 4 cut(s) | 203, 1245, 1279, 1859 |
| Sau96I | GGNCC | 7 cut(s) | 127, 461, 747, 800, 893, 1323, 1572 |
| SchI | GAGTC | 2 cut(s) | 982, 2093 |
| ScrFI | CCNGG | 3 cut(s) | 275, 612, 911 |
| SduI | GDGCHC | 3 cut(s) | 1170, 1654, 1946 |
| SfaNI | GCATC | 8 cut(s) | 235, 660, 1063, 1215, 1342, 1395, 1453, 1818 |
| SfuI | TTCGAA | 1 cut(s) | 839 |
| SinI | GGWCC | 6 cut(s) | 127, 461, 747, 800, 893, 1572 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 172, 1220 |
| SmlI | CTYRAG | 4 cut(s) | 233, 1229, 1712, 2129 |
| SmoI | CTYRAG | 4 cut(s) | 233, 1229, 1712, 2129 |
| SpeI | ACTAGT | 1 cut(s) | 349 |
| SphI | GCATGC | 1 cut(s) | 1782 |
| SsiI | CCGC | 2 cut(s) | 464, 1488 |
| SstI | GAGCTC | 1 cut(s) | 1654 |
| StyD4I | CCNGG | 3 cut(s) | 273, 610, 909 |
| StyI | CCWWGG | 2 cut(s) | 1138, 1855 |
| TaaI | ACNGT | 3 cut(s) | 127, 827, 2113 |
| TaqI | TCGA | 1 cut(s) | 839 |
| TatI | WGTACW | 4 cut(s) | 164, 395, 1548, 1865 |
| TauI | GCSGC | 1 cut(s) | 1490 |
| TfiI | GAWTC | 7 cut(s) | 41, 562, 752, 794, 836, 1615, 1805 |
| Tru1I | TTAA | 8 cut(s) | 413, 699, 960, 1161, 1230, 1713, 1892, 2115 |
| Tru9I | TTAA | 8 cut(s) | 413, 699, 960, 1161, 1230, 1713, 1892, 2115 |
| TscAI | CASTG | 3 cut(s) | 79, 825, 925 |
| TseFI | GTSAC | 1 cut(s) | 1309 |
| TseI | GCWGC | 3 cut(s) | 593, 785, 944 |
| Tsp45I | GTSAC | 1 cut(s) | 1309 |
| TspGWI | ACGGA | 1 cut(s) | 1279 |
| TspRI | CASTG | 3 cut(s) | 79, 825, 925 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 611 |
| Vha464I | CTTAAG | 2 cut(s) | 1229, 1712 |
| VpaK11BI | GGWCC | 6 cut(s) | 127, 461, 747, 800, 893, 1572 |
| VspI | ATTAAT | 1 cut(s) | 960 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 144 |
| XapI | RAATTY | 4 cut(s) | 116, 1123, 1532, 1790 |
| XbaI | TCTAGA | 1 cut(s) | 1258 |
| XceI | RCATGY | 3 cut(s) | 905, 1080, 1782 |
| XcmI | CCANNNNNNNNNTGG | 4 cut(s) | 79, 981, 1839, 1842 |
| Zsp2I | ATGCAT | 1 cut(s) | 1078 |