Rh5DG127300

Protein of unknown function (DUF 659)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000421
Physical Location & Seq
Forward (+)
11674126 .. 11674866
741 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG127300.1

Sequence Viewer

Length: 741 bp
ATGGCGTTTGGAAAAAGAAAACCATCCCGTCAAGAACCAATTTTTGAAGAAAATTATGAAGGTGATGTCGGCGAGCATGAAGACAATAATATTGTTGAAGAAACTCAAGCAAGTAATACAGAGTCTACAGACCAATCATCGAGTAGAAACAGCCATGAACAAGCTCAAGAATCTCAAGAAATTCCAATGAATTCTGACATAAAGCCATTACTCAATGAAGTTATTATCGCCAGTGGAGCGCAAGGAAAAAATTCAGGTGGAACAAAGACATGGACCTACAAACATTGTAATATTCAGTTTAAGAGTTCATATACTCGCATTCACTACCACTTTTTTGGTAATCTCCCTGGAAAAAAATCAGAGATTCGTCGATGTCAATCATTGATTAATAATCGGGAGGAATATGAGAGGGTTCGCAGAATGGTTATGGAAGCTGAAAAAAAAGGAGTTTCTTCATCTTTGAAAAGCTCAACAATCTTGAGGCAACAACCGCAAAAATCCACCGCAGGTGCTCTTGATGACATGTTCAAGATTATGGACAAAAATATTGTTGATATGAAGGTGATGAGAGGATTGTGCGCTAATGGAATTCCATTTAATGTGTTAAGAAATCCCCAATTTCATGAGATGATTTCAGCTATAAATCATGGACCCAGGGGTTATAAGGCTCCATCTTCTGAAAAAGCAAGAACTACTTTGCTTGATGAGTGTAAACGAAGTCTGGAAAAAGAATTAGCTCCT
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT

Protein Analysis

247

Amino Acids

28.03

Weight (kDa)

8.58

Isoelectric Point (pI)

51.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000421)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08267
fragaria_vesca FvH4_2g14331 FvH4_2g16371 FvH4_2g26163 FvH4_3g42540 FvH4_4g02102 FvH4_4g12491 FvH4_4g35171 FvH4_5g06632 FvH4_5g06632 FvH4_5g06632 FvH4_5g08811 FvH4_5g08812 FvH4_5g23531 FvH4_6g10272 FvH4_6g40844 FvH4_7g16803 FvH4_7g16804
malus_domestica MD11G1099500.v1.1 MD17G1176800.v1.1
pyrus_communis pycom05g07840 pycom06g08730 pycom07g12010 pycom07g12020 pycom10g07270 pycom11g17470 pycom12g10610 pycom12g14240 pycom13g22040 pycom15g02440
rosa_chinensis RchiOBHm_Chr1g0371321 RchiOBHm_Chr2g0102701 RchiOBHm_Chr3g0465681 RchiOBHm_Chr4g0420941 RchiOBHm_Chr4g0434271 RchiOBHm_Chr4g0442721 RchiOBHm_Chr6g0262081 RchiOBHm_Chr6g0278171 RchiOBHm_Chr6g0294991 RchiOBHm_Chr6g0295001 RchiOBHm_Chr7g0205361 RchiOBHm_Chr7g0205371 RchiOBHm_Chr7g0223471
rosa_laevigata RLG00000005266
rosa_multiflora Rmu_sc0000493.1_g000033 Rmu_sc0000493.1_g000034 Rmu_sc0000507.1_g000007 Rmu_sc0000843.1_g000001 Rmu_sc0001075.1_g000033 Rmu_sc0002077.1_g000007 Rmu_sc0002077.1_g000008 Rmu_sc0002329.1_g000029 Rmu_sc0002539.1_g000077 Rmu_sc0002655.1_g000006 Rmu_sc0003127.1_g000020 Rmu_sc0003765.1_g000054 Rmu_sc0004368.1_g000023 Rmu_sc0004753.1_g000022 Rmu_sc0005297.1_g000014 Rmu_sc0007633.1_g000010 Rmu_sc0008164.1_g000005 Rmu_sc0008191.1_g000003 Rmu_sc0017900.1_g000005 Rmu_sc0022727.1_g000002
rosa_roxburghii Rroxscaffold_2G00079920 Rroxscaffold_3G00255890 Rroxscaffold_4G00295340 Rroxscaffold_4G00296360 Rroxscaffold_4G00299640 Rroxscaffold_6G00388210
rosa_rugosa Rorug02G0466700 Rorug04G0123200 Rorug05G0074800 Rorug07G0202900
rosa_samantha Rh1DG134700 Rh3CG159000 Rh4AG331000 Rh5AG521100 Rh5BG136400 Rh5DG127300 Rh5DG383100 Rh5DG386100 Rh5DG458000 Rh6CG450400 Rh7CG233800 Rh7DG407100
rosa_wichuraiana Rw1G004960 Rw1G005730 Rw1G006360 Rw1G017400 Rw2G013190 Rw2G022210 Rw2G029640 Rw5G020910 Rw5G036930 Rw6G016200 Rw7G018170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 663
AarI CACCTGC 1 cut(s) 497
Acc36I ACCTGC 1 cut(s) 497
AccI GTMKAC 1 cut(s) 125
AciI CCGC 2 cut(s) 491, 504
AcsI RAATTY 4 cut(s) 180, 190, 250, 588
AflIII ACRYGT 1 cut(s) 522
AgsI TTSAA 4 cut(s) 47, 98, 463, 529
AjnI CCWGG 2 cut(s) 346, 653
AluBI AGCT 5 cut(s) 164, 434, 468, 638, 737
AluI AGCT 5 cut(s) 164, 434, 468, 638, 737
Alw21I GWGCWC 1 cut(s) 514
ApoI RAATTY 4 cut(s) 180, 190, 250, 588
AseI ATTAAT 1 cut(s) 387
Asp700I GAANNNNTTC 1 cut(s) 250
AspLEI GCGC 2 cut(s) 241, 581
AspS9I GGNCC 2 cut(s) 273, 650
AsuHPI GGTGA 2 cut(s) 74, 574
AvaII GGWCC 2 cut(s) 273, 650
BbsI GAAGAC 1 cut(s) 87
Bbv12I GWGCWC 1 cut(s) 514
BccI CCATC 2 cut(s) 31, 679
BciT130I CCWGG 2 cut(s) 348, 655
BfmI CTRYAG 1 cut(s) 126
BfuAI ACCTGC 1 cut(s) 497
Bme1390I CCNGG 2 cut(s) 348, 655
Bme18I GGWCC 2 cut(s) 273, 650
BmgT120I GGNCC 2 cut(s) 273, 650
BmiI GGNNCC 2 cut(s) 652, 669
BmrFI CCNGG 2 cut(s) 348, 655
BpiI GAAGAC 1 cut(s) 87
BpuEI CTTGAG 4 cut(s) 90, 150, 159, 499
BsaBI GATNNNNATC 1 cut(s) 376
BsaJI CCNNGG 3 cut(s) 346, 653, 654
Bse1I ACTGG 1 cut(s) 231
Bse8I GATNNNNATC 1 cut(s) 376
BseBI CCWGG 2 cut(s) 348, 655
BseDI CCNNGG 3 cut(s) 346, 653, 654
BseGI GGATG 1 cut(s) 23
BseJI GATNNNNATC 1 cut(s) 376
BseNI ACTGG 1 cut(s) 231
BsiHKAI GWGCWC 1 cut(s) 514
BsmI GAATGC 1 cut(s) 318
Bsp1286I GDGCHC 1 cut(s) 514
BspACI CCGC 2 cut(s) 491, 504
BspHI TCATGA 1 cut(s) 622
BspLI GGNNCC 2 cut(s) 652, 669
BspMI ACCTGC 1 cut(s) 497
BsrI ACTGG 1 cut(s) 231
BssECI CCNNGG 3 cut(s) 346, 653, 654
Bst2UI CCWGG 2 cut(s) 348, 655
BstC8I GCNNGC 1 cut(s) 74
BstF5I GGATG 1 cut(s) 23
BstHHI GCGC 2 cut(s) 241, 581
BstMWI GCNNNNNNNGC 2 cut(s) 236, 490
BstNI CCWGG 2 cut(s) 348, 655
BstNSI RCATGY 1 cut(s) 526
BstSCI CCNGG 2 cut(s) 346, 653
BstSFI CTRYAG 1 cut(s) 126
BstV2I GAAGAC 1 cut(s) 87
BstXI CCANNNNNNTGG 1 cut(s) 335
BtsCI GGATG 1 cut(s) 23
BtsIMutI CAGTG 1 cut(s) 238
BveI ACCTGC 1 cut(s) 497
Cac8I GCNNGC 1 cut(s) 74
CciI TCATGA 1 cut(s) 622
CfoI GCGC 2 cut(s) 241, 581
Cfr13I GGNCC 2 cut(s) 273, 650
CviAII CATG 6 cut(s) 77, 155, 270, 523, 623, 647
CviJI RGCY 8 cut(s) 153, 164, 205, 434, 468, 638, 668, 737
CviKI_1 RGCY 8 cut(s) 153, 164, 205, 434, 468, 638, 668, 737
Eco47I GGWCC 2 cut(s) 273, 650
EcoRI GAATTC 2 cut(s) 190, 588
EcoRII CCWGG 2 cut(s) 346, 653
FaeI CATG 6 cut(s) 80, 158, 273, 526, 626, 650
FalI AAGNNNNNCTT 2 cut(s) 679, 711
FatI CATG 6 cut(s) 76, 154, 269, 522, 622, 646
FblI GTMKAC 1 cut(s) 125
FokI GGATG 1 cut(s) 10
GlaI GCGC 2 cut(s) 240, 580
HhaI GCGC 2 cut(s) 241, 581
Hin1II CATG 6 cut(s) 80, 158, 273, 526, 626, 650
Hin6I GCGC 2 cut(s) 239, 579
HinP1I GCGC 2 cut(s) 239, 579
HinfI GANTC 3 cut(s) 122, 170, 364
HphI GGTGA 2 cut(s) 74, 574
Hpy166II GTNNAC 2 cut(s) 126, 713
Hpy188I TCNGA 3 cut(s) 196, 361, 679
Hpy188III TCNNGA 9 cut(s) 32, 167, 176, 395, 478, 515, 529, 623, 722
Hpy8I GTNNAC 2 cut(s) 126, 713
Hpy99I CGWCG 1 cut(s) 372
HpyAV CCTTC 2 cut(s) 53, 553
HpyF10VI GCNNNNNNNGC 2 cut(s) 236, 490
Hsp92II CATG 6 cut(s) 80, 158, 273, 526, 626, 650
HspAI GCGC 2 cut(s) 239, 579
LmnI GCTCC 2 cut(s) 236, 673
LpnPI CCDG 8 cut(s) 240, 244, 333, 360, 492, 640, 667, 707
MboII GAAGA 5 cut(s) 59, 92, 110, 444, 666
MhlI GDGCHC 1 cut(s) 514
MluCI AATT 8 cut(s) 39, 52, 180, 190, 250, 588, 617, 731
MlyI GAGTC 1 cut(s) 131
MnlI CCTC 4 cut(s) 391, 402, 474, 563
MroXI GAANNNNTTC 1 cut(s) 250
MseI TTAA 4 cut(s) 300, 387, 597, 605
MspR9I CCNGG 2 cut(s) 348, 655
Mva1269I GAATGC 1 cut(s) 318
MvaI CCWGG 2 cut(s) 348, 655
MwoI GCNNNNNNNGC 2 cut(s) 236, 490
NlaIII CATG 6 cut(s) 80, 158, 273, 526, 626, 650
NlaIV GGNNCC 2 cut(s) 652, 669
NspI RCATGY 1 cut(s) 526
PagI TCATGA 1 cut(s) 622
PaqCI CACCTGC 1 cut(s) 497
PasI CCCWGGG 1 cut(s) 654
PciI ACATGT 1 cut(s) 522
PctI GAATGC 1 cut(s) 318
PdmI GAANNNNTTC 1 cut(s) 250
PfeI GAWTC 2 cut(s) 170, 364
PleI GAGTC 1 cut(s) 130
PpsI GAGTC 1 cut(s) 130
PscI ACATGT 1 cut(s) 522
PshBI ATTAAT 1 cut(s) 387
PsiI TTATAA 1 cut(s) 663
Psp6I CCWGG 2 cut(s) 346, 653
PspGI CCWGG 2 cut(s) 346, 653
PspN4I GGNNCC 2 cut(s) 652, 669
PspPI GGNCC 2 cut(s) 273, 650
SaqAI TTAA 4 cut(s) 300, 387, 597, 605
Sau96I GGNCC 2 cut(s) 273, 650
SchI GAGTC 1 cut(s) 131
ScrFI CCNGG 2 cut(s) 348, 655
SduI GDGCHC 1 cut(s) 514
SfcI CTRYAG 1 cut(s) 126
SinI GGWCC 2 cut(s) 273, 650
SmlI CTYRAG 4 cut(s) 105, 165, 174, 478
SmoI CTYRAG 4 cut(s) 105, 165, 174, 478
Sse9I AATT 8 cut(s) 39, 52, 180, 190, 250, 588, 617, 731
SsiI CCGC 2 cut(s) 491, 504
SspI AATATT 3 cut(s) 91, 292, 547
StyD4I CCNGG 2 cut(s) 346, 653
TaqI TCGA 2 cut(s) 140, 370
TasI AATT 8 cut(s) 39, 52, 180, 190, 250, 588, 617, 731
TfiI GAWTC 2 cut(s) 170, 364
Tru1I TTAA 4 cut(s) 300, 387, 597, 605
Tru9I TTAA 4 cut(s) 300, 387, 597, 605
TscAI CASTG 1 cut(s) 238
TspDTI ATGAA 9 cut(s) 72, 93, 171, 203, 231, 297, 444, 572, 611
TspRI CASTG 1 cut(s) 238
VpaK11BI GGWCC 2 cut(s) 273, 650
VspI ATTAAT 1 cut(s) 387
XapI RAATTY 4 cut(s) 180, 190, 250, 588
XceI RCATGY 1 cut(s) 526
XmiI GTMKAC 1 cut(s) 125
XmnI GAANNNNTTC 1 cut(s) 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.