MD17G1176800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000421
Physical Location & Seq
Forward (+)
20086461 .. 20088454
1994 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1176800.v1.1.491

Sequence Viewer

Length: 1254 bp
ATGGCTGGAAACGGGGTCGCGCCTTGCAACAAGGTTACTGATGAAAATCTTGCAGAAATGAAAAAGTTAATTCGAGAGTGTGAATATAAGCTGAAGAGTTCTGCTCCTAAGAATGTTTCTTTGCCCAATACTGGTTCTTTTTGTTATGATCTACCCGAAGTGAATCTTGATACAAAGAAGAGAAAGGGAACAAGTGGGCCTCTTAGTAAAGCTTTTAACAAGGAAGCTCGAGATCAATGTGATGCTGAAGTTGCAAGGATGTTTTATACAGGTGGCTTATCATTTAACCTTGCAAGAAATCCGCACTATCGAAACTCATATGTTCGAGCTTCTACACTTCCAGGGTATGTTCCACCAGGTTACAATGCACTACGAACTACTCTTCTGCAACAAGAGAAGAGTCACATTGAGCAATGCCTCCAACCAATCAAGCGCACATGGAGCACTAAAGGTGTAAGTTTGTGCAGTGATGGGTGGACAGATGCACAAAGGAGACCACTTATTAATATTATGGCAACTTGTGAAAGCGGGCCTATGTTCTTGAGGGCAATTAATTGTGAAGCTGCTGGGTTACTTATTGAGGCCCACTATCCCCATATCTTTTGGACACCCTGTGTTGTTCACACTCTTAATCTTGCTTTGAAGAGTATATGCTCTCCGAAACAAACAGATGTTTCGTACGATGACTGCAATTGGATTTCAACTATTGCTAGCGATGTTTGGTCCATAAAAAATTTTATTATGAACCATAGCATGAGATTGTCTATGTTTAATGAACATTGCAAACTAAAGTTGCTCTCCATTGCCGAAACAAGATTTGCTTCCACACTTGTGATGCTTAGAAGATTTAAAGAAGTAAAGGAAGGTTTACAACAAATGGTGATTAGCCCGAATTGGGCTTTGTACAAAGAAGATGATTTGGTTAAAGCAATGACGGTGAAGCAAAAAATATTGGATGAGTACTTTTGGGAGAAGATTGATTATATTCTTTTCTTTACAGCTCCAATATATGAGGCTATTAGAATGGCTGACACAGATAAACCTTGTCTTCACTTGGTGTATGAGTGGTGGGATGCTATGATTGAAAAGGTGAAAGCTGCTATCTACAGGAATGAGCGCAAGGCATTACATGAAAAAAACAGCTTTTTTGATGTGGTGTATCGCATTTTATTAGAGCGATGGACTAAAAGTAGCACACCACTTCATTGTTTGGCGCATTCATTAAATCCAAGAATGGCTCCAAGAAGACCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT

Protein Analysis

418

Amino Acids

47.93

Weight (kDa)

8.98

Isoelectric Point (pI)

37.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF659 PF04937 119 - 187 5.9e-18 Domain of unknown function (DUF659)
DUF659 PF04937 185 - 240 2.5e-08 Domain of unknown function (DUF659)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000421)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08267
fragaria_vesca FvH4_2g14331 FvH4_2g16371 FvH4_2g26163 FvH4_3g42540 FvH4_4g02102 FvH4_4g12491 FvH4_4g35171 FvH4_5g06632 FvH4_5g06632 FvH4_5g06632 FvH4_5g08811 FvH4_5g08812 FvH4_5g23531 FvH4_6g10272 FvH4_6g40844 FvH4_7g16803 FvH4_7g16804
malus_domestica MD11G1099500.v1.1 MD17G1176800.v1.1
pyrus_communis pycom05g07840 pycom06g08730 pycom07g12010 pycom07g12020 pycom10g07270 pycom11g17470 pycom12g10610 pycom12g14240 pycom13g22040 pycom15g02440
rosa_chinensis RchiOBHm_Chr1g0371321 RchiOBHm_Chr2g0102701 RchiOBHm_Chr3g0465681 RchiOBHm_Chr4g0420941 RchiOBHm_Chr4g0434271 RchiOBHm_Chr4g0442721 RchiOBHm_Chr6g0262081 RchiOBHm_Chr6g0278171 RchiOBHm_Chr6g0294991 RchiOBHm_Chr6g0295001 RchiOBHm_Chr7g0205361 RchiOBHm_Chr7g0205371 RchiOBHm_Chr7g0223471
rosa_laevigata RLG00000005266
rosa_multiflora Rmu_sc0000493.1_g000033 Rmu_sc0000493.1_g000034 Rmu_sc0000507.1_g000007 Rmu_sc0000843.1_g000001 Rmu_sc0001075.1_g000033 Rmu_sc0002077.1_g000007 Rmu_sc0002077.1_g000008 Rmu_sc0002329.1_g000029 Rmu_sc0002539.1_g000077 Rmu_sc0002655.1_g000006 Rmu_sc0003127.1_g000020 Rmu_sc0003765.1_g000054 Rmu_sc0004368.1_g000023 Rmu_sc0004753.1_g000022 Rmu_sc0005297.1_g000014 Rmu_sc0007633.1_g000010 Rmu_sc0008164.1_g000005 Rmu_sc0008191.1_g000003 Rmu_sc0017900.1_g000005 Rmu_sc0022727.1_g000002
rosa_roxburghii Rroxscaffold_2G00079920 Rroxscaffold_3G00255890 Rroxscaffold_4G00295340 Rroxscaffold_4G00296360 Rroxscaffold_4G00299640 Rroxscaffold_6G00388210
rosa_rugosa Rorug02G0466700 Rorug04G0123200 Rorug05G0074800 Rorug07G0202900
rosa_samantha Rh1DG134700 Rh3CG159000 Rh4AG331000 Rh5AG521100 Rh5BG136400 Rh5DG127300 Rh5DG383100 Rh5DG386100 Rh5DG458000 Rh6CG450400 Rh7CG233800 Rh7DG407100
rosa_wichuraiana Rw1G004960 Rw1G005730 Rw1G006360 Rw1G017400 Rw2G013190 Rw2G022210 Rw2G029640 Rw5G020910 Rw5G036930 Rw6G016200 Rw7G018170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 20
AciI CCGC 2 cut(s) 302, 528
AcsI RAATTY 1 cut(s) 733
AcuI CTGAAG 2 cut(s) 113, 267
AdeI CACNNNGTG 2 cut(s) 614, 1057
AfaI GTAC 3 cut(s) 680, 905, 962
AfiI CCNNNNNNNGG 2 cut(s) 131, 895
AgsI TTSAA 3 cut(s) 643, 702, 1085
AjnI CCWGG 2 cut(s) 340, 355
AleI CACNNNNGTG 1 cut(s) 830
AluBI AGCT 8 cut(s) 91, 212, 227, 329, 563, 1001, 1097, 1143
AluI AGCT 8 cut(s) 91, 212, 227, 329, 563, 1001, 1097, 1143
Alw21I GWGCWC 1 cut(s) 446
Alw26I GTCTC 1 cut(s) 487
Ama87I CYCGRG 1 cut(s) 228
AoxI GGCC 3 cut(s) 197, 530, 582
ApeKI GCWGC 2 cut(s) 563, 1097
ApoI RAATTY 1 cut(s) 733
AseI ATTAAT 2 cut(s) 504, 552
AspLEI GCGC 4 cut(s) 22, 435, 1119, 1216
AspS9I GGNCC 4 cut(s) 197, 530, 583, 723
AsuHPI GGTGA 3 cut(s) 892, 949, 1102
AsuNHI GCTAGC 1 cut(s) 710
AvaI CYCGRG 1 cut(s) 228
AvaII GGWCC 1 cut(s) 723
BbsI GAAGAC 1 cut(s) 1040
Bbv12I GWGCWC 1 cut(s) 446
BbvI GCAGC 2 cut(s) 550, 1084
BccI CCATC 2 cut(s) 464, 1173
BciT130I CCWGG 2 cut(s) 342, 357
BcoDI GTCTC 1 cut(s) 487
BfaI CTAG 2 cut(s) 711, 1252
BfmI CTRYAG 1 cut(s) 1105
BisI GCNGC 2 cut(s) 564, 1098
BlsI GCNGC 2 cut(s) 565, 1099
BmcAI AGTACT 1 cut(s) 962
Bme1390I CCNGG 2 cut(s) 342, 357
Bme18I GGWCC 1 cut(s) 723
BmeT110I CYCGRG 1 cut(s) 228
BmgT120I GGNCC 4 cut(s) 197, 530, 583, 723
BmiI GGNNCC 1 cut(s) 1239
BmrFI CCNGG 2 cut(s) 342, 357
BmsI GCATC 4 cut(s) 232, 472, 825, 1063
BmtI GCTAGC 1 cut(s) 714
BpiI GAAGAC 1 cut(s) 1040
BplI GAGNNNNNCTC 2 cut(s) 88, 120
BpuEI CTTGAG 1 cut(s) 562
BsaBI GATNNNNATC 1 cut(s) 45
BsaI GGTCTC 1 cut(s) 487
BsaJI CCNNGG 1 cut(s) 341
Bsc4I CCNNNNNNNGG 2 cut(s) 131, 895
Bse1I ACTGG 1 cut(s) 136
Bse3DI GCAATG 4 cut(s) 419, 778, 801, 936
Bse8I GATNNNNATC 1 cut(s) 45
BseBI CCWGG 2 cut(s) 342, 357
BseDI CCNNGG 1 cut(s) 341
BseGI GGATG 3 cut(s) 264, 961, 1078
BseJI GATNNNNATC 1 cut(s) 45
BseLI CCNNNNNNNGG 2 cut(s) 131, 895
BseMI GCAATG 4 cut(s) 419, 778, 801, 936
BseNI ACTGG 1 cut(s) 136
BseXI GCAGC 2 cut(s) 550, 1084
BseYI CCCAGC 1 cut(s) 566
BsgI GTGCAG 1 cut(s) 484
Bsh1236I CGCG 1 cut(s) 20
BshFI GGCC 3 cut(s) 199, 532, 584
BsiHKAI GWGCWC 1 cut(s) 446
BsiHKCI CYCGRG 1 cut(s) 228
BsiWI CGTACG 1 cut(s) 678
BslI CCNNNNNNNGG 2 cut(s) 131, 895
BsmAI GTCTC 1 cut(s) 487
BsmI GAATGC 1 cut(s) 1216
BsnI GGCC 3 cut(s) 199, 532, 584
Bso31I GGTCTC 1 cut(s) 487
BsoBI CYCGRG 1 cut(s) 228
Bsp1286I GDGCHC 1 cut(s) 446
Bsp1407I TGTACA 1 cut(s) 903
Bsp143I GATC 2 cut(s) 148, 232
BspACI CCGC 2 cut(s) 302, 528
BspANI GGCC 3 cut(s) 199, 532, 584
BspFNI CGCG 1 cut(s) 20
BspLI GGNNCC 1 cut(s) 1239
BspOI GCTAGC 1 cut(s) 714
BspTNI GGTCTC 1 cut(s) 487
BsrDI GCAATG 4 cut(s) 419, 778, 801, 936
BsrGI TGTACA 1 cut(s) 903
BsrI ACTGG 1 cut(s) 136
BssECI CCNNGG 1 cut(s) 341
BssMI GATC 2 cut(s) 148, 232
Bst2UI CCWGG 2 cut(s) 342, 357
Bst4CI ACNGT 1 cut(s) 937
Bst6I CTCTTC 5 cut(s) 89, 173, 387, 392, 638
BstAUI TGTACA 1 cut(s) 903
BstC8I GCNNGC 2 cut(s) 530, 712
BstDEI CTNAG 3 cut(s) 108, 203, 839
BstF5I GGATG 3 cut(s) 264, 961, 1078
BstFNI CGCG 1 cut(s) 20
BstHHI GCGC 4 cut(s) 22, 435, 1119, 1216
BstKTI GATC 2 cut(s) 151, 235
BstMAI GTCTC 1 cut(s) 487
BstMBI GATC 2 cut(s) 148, 232
BstMWI GCNNNNNNNGC 2 cut(s) 251, 441
BstNI CCWGG 2 cut(s) 342, 357
BstSCI CCNGG 2 cut(s) 340, 355
BstSFI CTRYAG 1 cut(s) 1105
BstUI CGCG 1 cut(s) 20
BstV1I GCAGC 2 cut(s) 550, 1084
BstV2I GAAGAC 1 cut(s) 1040
BsuRI GGCC 3 cut(s) 199, 532, 584
BtgZI GCGATG 2 cut(s) 729, 1192
BtsCI GGATG 3 cut(s) 264, 961, 1078
BtsI GCAGTG 1 cut(s) 472
BtsIMutI CAGTG 1 cut(s) 472
Cac8I GCNNGC 2 cut(s) 530, 712
CfoI GCGC 4 cut(s) 22, 435, 1119, 1216
Cfr13I GGNCC 4 cut(s) 197, 530, 583, 723
CsiI ACCWGGT 1 cut(s) 355
Csp6I GTAC 3 cut(s) 679, 904, 961
CviAII CATG 3 cut(s) 438, 754, 1130
CviQI GTAC 3 cut(s) 679, 904, 961
DdeI CTNAG 3 cut(s) 108, 203, 839
DpnI GATC 2 cut(s) 150, 234
DpnII GATC 2 cut(s) 148, 232
DraI TTTAAA 1 cut(s) 850
DraIII CACNNNGTG 2 cut(s) 614, 1057
Eam1104I CTCTTC 5 cut(s) 89, 173, 387, 392, 638
EarI CTCTTC 5 cut(s) 89, 173, 387, 392, 638
Eco31I GGTCTC 1 cut(s) 487
Eco47I GGWCC 1 cut(s) 723
Eco57I CTGAAG 2 cut(s) 113, 267
Eco88I CYCGRG 1 cut(s) 228
EcoRII CCWGG 2 cut(s) 340, 355
FaeI CATG 3 cut(s) 441, 757, 1133
FalI AAGNNNNNCTT 4 cut(s) 150, 182, 805, 837
FatI CATG 3 cut(s) 437, 753, 1129
FauI CCCGC 1 cut(s) 521
FauNDI CATATG 1 cut(s) 319
Fnu4HI GCNGC 2 cut(s) 564, 1098
FokI GGATG 3 cut(s) 271, 968, 1085
Fsp4HI GCNGC 2 cut(s) 564, 1098
FspBI CTAG 2 cut(s) 711, 1252
GlaI GCGC 4 cut(s) 21, 434, 1118, 1215
GluI GCNGC 2 cut(s) 564, 1098
GsaI CCCAGC 1 cut(s) 570
HaeIII GGCC 3 cut(s) 199, 532, 584
HhaI GCGC 4 cut(s) 22, 435, 1119, 1216
Hin1II CATG 3 cut(s) 441, 757, 1133
Hin6I GCGC 4 cut(s) 20, 433, 1117, 1214
HinP1I GCGC 4 cut(s) 20, 433, 1117, 1214
HindIII AAGCTT 1 cut(s) 210
HinfI GANTC 2 cut(s) 163, 400
HphI GGTGA 3 cut(s) 892, 949, 1102
Hpy166II GTNNAC 3 cut(s) 477, 622, 869
Hpy188I TCNGA 1 cut(s) 660
Hpy188III TCNNGA 4 cut(s) 74, 167, 230, 541
Hpy8I GTNNAC 3 cut(s) 477, 622, 869
HpyAV CCTTC 1 cut(s) 857
HpyCH4III ACNGT 1 cut(s) 937
HpyF10VI GCNNNNNNNGC 2 cut(s) 251, 441
HpyF3I CTNAG 3 cut(s) 108, 203, 839
Hsp92II CATG 3 cut(s) 441, 757, 1133
HspAI GCGC 4 cut(s) 20, 433, 1117, 1214
Kzo9I GATC 2 cut(s) 148, 232
LmnI GCTCC 4 cut(s) 109, 441, 1006, 1243
LpnPI CCDG 9 cut(s) 117, 255, 327, 342, 354, 369, 552, 625, 1093
Lsp1109I GCAGC 2 cut(s) 550, 1084
LweI GCATC 4 cut(s) 232, 472, 825, 1063
MabI ACCWGGT 1 cut(s) 355
MaeI CTAG 2 cut(s) 711, 1252
MaeIII GTNAC 4 cut(s) 34, 359, 401, 570
MalI GATC 2 cut(s) 150, 234
MboI GATC 2 cut(s) 148, 232
MboII GAAGA 9 cut(s) 106, 190, 374, 409, 655, 855, 923, 985, 1040
MfeI CAATTG 1 cut(s) 691
MhlI GDGCHC 1 cut(s) 446
MluCI AATT 6 cut(s) 69, 549, 553, 691, 733, 892
MlyI GAGTC 1 cut(s) 409
MmeI TCCRAC 1 cut(s) 445
MnlI CCTC 5 cut(s) 210, 428, 537, 574, 1006
MslI CAYNNNNRTG 1 cut(s) 830
MspR9I CCNGG 2 cut(s) 342, 357
MunI CAATTG 1 cut(s) 691
Mva1269I GAATGC 1 cut(s) 1216
MvaI CCWGG 2 cut(s) 342, 357
MvnI CGCG 1 cut(s) 20
MwoI GCNNNNNNNGC 2 cut(s) 251, 441
NdeI CATATG 1 cut(s) 319
NdeII GATC 2 cut(s) 148, 232
NheI GCTAGC 1 cut(s) 710
NlaIII CATG 3 cut(s) 441, 757, 1133
NlaIV GGNNCC 1 cut(s) 1239
NmuCI GTSAC 1 cut(s) 401
OliI CACNNNNGTG 1 cut(s) 830
PaeR7I CTCGAG 1 cut(s) 228
PctI GAATGC 1 cut(s) 1216
PfeI GAWTC 1 cut(s) 163
Pfl23II CGTACG 1 cut(s) 678
PkrI GCNGC 2 cut(s) 565, 1099
PleI GAGTC 1 cut(s) 408
PpsI GAGTC 1 cut(s) 408
PshBI ATTAAT 2 cut(s) 504, 552
Psp6I CCWGG 2 cut(s) 340, 355
PspFI CCCAGC 1 cut(s) 566
PspGI CCWGG 2 cut(s) 340, 355
PspLI CGTACG 1 cut(s) 678
PspN4I GGNNCC 1 cut(s) 1239
PspPI GGNCC 4 cut(s) 197, 530, 583, 723
RsaI GTAC 3 cut(s) 680, 905, 962
RsaNI GTAC 3 cut(s) 679, 904, 961
RseI CAYNNNNRTG 1 cut(s) 830
SatI GCNGC 2 cut(s) 564, 1098
Sau3AI GATC 2 cut(s) 148, 232
Sau96I GGNCC 4 cut(s) 197, 530, 583, 723
ScaI AGTACT 1 cut(s) 962
SchI GAGTC 1 cut(s) 409
ScrFI CCNGG 2 cut(s) 342, 357
SduI GDGCHC 1 cut(s) 446
SexAI ACCWGGT 1 cut(s) 355
SfaNI GCATC 4 cut(s) 232, 472, 825, 1063
SfcI CTRYAG 1 cut(s) 1105
Sfr274I CTCGAG 1 cut(s) 228
SinI GGWCC 1 cut(s) 723
SlaI CTCGAG 1 cut(s) 228
SmiMI CAYNNNNRTG 1 cut(s) 830
SmlI CTYRAG 2 cut(s) 228, 541
SmoI CTYRAG 2 cut(s) 228, 541
Sse9I AATT 6 cut(s) 69, 549, 553, 691, 733, 892
SsiI CCGC 2 cut(s) 302, 528
SspI AATATT 2 cut(s) 508, 951
SspMI CTAG 2 cut(s) 711, 1252
StyD4I CCNGG 2 cut(s) 340, 355
TaaI ACNGT 1 cut(s) 937
TaqI TCGA 4 cut(s) 73, 229, 310, 325
TasI AATT 6 cut(s) 69, 549, 553, 691, 733, 892
TatI WGTACW 2 cut(s) 903, 960
TfiI GAWTC 1 cut(s) 163
TscAI CASTG 1 cut(s) 472
TseFI GTSAC 1 cut(s) 401
TseI GCWGC 2 cut(s) 563, 1097
Tsp45I GTSAC 1 cut(s) 401
TspDTI ATGAA 7 cut(s) 57, 74, 758, 789, 1146, 1193, 1209
TspRI CASTG 1 cut(s) 472
VpaK11BI GGWCC 1 cut(s) 723
VspI ATTAAT 2 cut(s) 504, 552
XapI RAATTY 1 cut(s) 733
XhoI CTCGAG 1 cut(s) 228
XspI CTAG 2 cut(s) 711, 1252
ZrmI AGTACT 1 cut(s) 962
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.