MD03G1137000.v1.1

Pleiotropic drug resistance protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000245
Physical Location & Seq
Reverse (-)
13790458 .. 13798646
8189 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1137000.v1.1.491

Sequence Viewer

Length: 4347 bp
ATGGCGGCTTTAGCCGGAGATGATCTATCTAGGCAGTCCAGTGTAGCCAGGCAGTCGAGCAGCCGGCGGAGCTGGCGGTCGACTAGTGTTCGGGAAATGTGGAACGCACCGGATGTGTTCCAGCGGAGCGGGCGGCAGCAGGCGGTGGATGAGGAGGAGGAGCTCAAGTGGGCCGCCATAGAGAGGCTGCCCACTTATGATAGGATGCGGAGGGGGATGCTGAGACAGGCCATGAGCAATGGGAGATTTGTAACTGAAGAAGTCGACGTTGCGAATCTTGGGGCGCAAGACAAGAAGCAGTTGATGGAGAGCATACTTAAGGTCGTGGAGGATGATAATGAAAGGTTCTTGCAGAGGCTCAGAGCCAGAAACGACAGGGTTGGAATTGAGGTTCCTAAGGTTGAGGTCAGATTCCAGAATTTGTCAATAGAAGGAGATGCTTATGTTGGCACCAGAGCACTTCCAACTCTGCTGAATTCCACCTTGAACCAACTTGAGGGACTAGTTGGATTGATTGGACTCTCAAAATCAAAGAAAAGAGTTGTCAAGATACTCCAAGATGTGAGCGGTATTGTTAAACCGTCAAGGATGACACTGCTTCTTGGACCTCCATCGTCAGGAAAAACAACACTGCTAAAAGCACTTGCTGGCAAACTCGATAAAGATCTAAGGGTAACTGGGAACGTGACCTATTGTGGCCATGAATTCCACGAATTTGTGCCTCAGAGAACTTCTGCTTATATTAGCCAGCATGATCTTCATTATGGTGAGATGACAGTTCGTGAAACATTGGATTTCTCCGGACGTTGCCTGGGAGTTGGAACCAGGTACGATATGCTGGTAGAGTTGTCTAGACGGGAGAAAGATTCAGGTATCAAACCCGATCCTGAAATCGACGCATTCATGAAAGCCACATCTATGGTTGGCCAGGAAACTAGTTTGATCACAGATTACGTTCTCAAGATACTTGGACTTGATATCTGTGCTGATATTATGGTGGGTGATGACATGAGAAGGGGCATATCCGGTGGACAGAAGAAACGTGTAACTACTGGAGAAATGTTGGTTGGACCAGCGAAAGCATTTTTCATGGATGAAATATCAACGGGGCTGGACAGTTCCACAACCTTTCAGATTGTCAAGTTCATGAGGCAGATGGTTCATATTATGGAAGTCTCAATGGTCATCTCTCTCTTGCAGCCTGCACCTGAGACGTATGATCTGTTTGACGACATTATGCTTCTTTCTGAGGGTCAGATTGTGTACCATGGTCCACGTGAAAATGTGCTTGAGTTCTTCGAATATATGGGGTTCAAATGCCCTGACAGAAAAGGTGTTGCAGACTTCTTGCAAGAAGTGACGTCTAAGAAGGACCAAGAACAATACTGGTTTAAGAAGAACCAACCCTACAGATTTGTTTCAGTATCCGATTTTGTTCGAGCATTCACCACTTTTCATGTTGGCCAACGTCTTGTGGAGGAACTAAGAGTTCCTTATGATAAAAGATCAGTCCATCCTGCTGCTCTGGTGACGGATAAGTATGGAATATCCAATATGGAGCTCTTCAAGGCATGCTTTGCAAGGGAATGGTTGCTGATGAAGCGTAACTCTTTCGTGTACGTATTCAAAACTACGCAGATAACAATCATGGCTACAATTGCTTTGACGGTATTCTTAAGAACAGAAATGAAAGCCGGAAGAGCAGAAGATTCGGCAAAATTCTGGGGAGCACTGTTTTTCAGTCTCATTAACGTCATGTTTAACGGAATGGCAGAGCTTGCAATGACAGTTTTCAGGATTCCTGTGTTCTTTAAACAAAGGGATGCCCTGTTCTATCCTGGCTGGGCTTTTGGCTTGCCCATTTCGCTAACCAGAATTCCCATTTCACTGATGGAATCTGGGATATGGATTGTTCTAACATACTACACCATCGGGTTTGCACCAGCGGCCAGTAGGTTCTTCAAACAGTTTTTGGCTTTCTTCGGCGTACATCAGATGGCACTCTCCCTTTTCCGTTTCATTGCTGCCCTTGGAAGAACAGAGGTTGTTTCGAATACAATTGGTTCCTTTACGTTGCTACTGGTGTTTGTCCTTGGAGGTTTCGTCGTCGCTAAAGATGACATCAAGCCGTGGATGATATGGGGCTACTATGCGTCACCTATGATGTATGGGCAAAATGCAATTGCTATCAATGAGTTTCTTGATAAAAGATGGAGTGCTCCTGTTCTCAATGCCTCTGAAAACACAGTTGGAAAGGTACTTCTGAGGGAAAGAGGACTGTTTACTACGGAGACTTGGTATTGGACTTGTATTGCCGCGCTCTTCGGATTTACTGTTCTCTTCAATATTCTTTTTATGGCAGCACTGACCTACTTAGATCCCCTAAGTGAAACTAAAACTCTAATTGAGAATGATGAAGACTCTGAAGGGAATAAGAAGCGACGATCCAGTCCAGAAGGTATTGATATGCAAGTCAGAAATTCTCAAGGAAATAATCAAGCCAAACGAGGAATGGTTTTACCCTTCCAGCCCCTCTCACTCGCTTTCAACCATGTGAATTACTATGTGGATATGCCTGCCGAAATGAAGAGCCAAGGGATTGAAGAGACTCGACTCCAACTTCTACGAGATGTCAGTGGTGCATTTAGACCTGGTGTTTTGACTGCATTAGTTGGTGTCAGTGGTGCTGGAAAGACAACCTTGATGGATGTCTTAGCTGGAAGAAAGACCGGTGGGTACATTGAAGGAAGTATTACCATCTCTGGTTACCCAAAGAACCAAGCCACATTTGCTCGGGTCAGCGGTTACTGTGAACAAAATGATATTCATTCACCATTTGTTACTGTCTATGAGTCTCTCCTATACTCTGCCTGGCTGCGTCTTGGTAAAGATGTCAAGACCGAAACAAGAAAGATGTTTGTTGATGAGGTTATGGATTTGGTCGAGCTTAACCCATTGAGAAATGCTTTAGTTGGACTTCCGGGAGTTGATGGACTTTCGACTGAGCAGAGAAAGAGGCTCACTATTGCTGTAGAATTGGTTGCCAACCCTTCCATCATCTTCATGGATGAGCCGACATCAGGACTTGATGCTAGAGCAGCCGCCATTGTTATGCGTACTGTAAGAAACACCGTGGATACAGGACGAACTGTTGTATGTACAATTCACCAGCCTAGCATTGACATTTTTGAAGCTTTTGATGAGCTTTTCTTAATGAAAAGAGGAGGACAAGTGATTTATGCCGGTCCTCTAGGTCGCCAGTCTCACAAACTTGTTGAATACTTTGAGGCTATTCCAGGGGTTGCAAAGATCAAAGAGGGTTACAATCCAGCTACCTGGATGTTGGAGGTCAGCTCGGCTGCCATTGAGGCTCAGAATGATGTGGACTTTGCAGAAATATTTGCAAATTCTGATCTGTATAAGAGAAATCAAGAACTTATCAAGGAACTAAGTATTCCAGAACCAGGCTCCAGCGATCTTTACTTCCCCACCCAATACTCCCAAAGCTTTGCAACACAATGCAAGGCATGCTTCTGGAAACAACACTGGTCATACTGGAGGAACTCACGATACAATGCCATTAGGTTTTTCATGACAATCTGCATTGGAGTATTATTTGGTGTTATCTTCTGGGGCAAAGGAGACAAGATACACAAACAACAAGACCTGATCAATCTTTTGGGAGCTACCTATTCTGCCATTCTCTTCCTTGGAGCTAGCAATGCTTCTGCTGTGCAATCTGTGGTTGCGGTTGAACGAACGGTCTTCTATCGTGAAAGAGCAGCCGGAATGTATTCAGAGTTGCCTTATGCATTTGCTCAGGTGGCTATTGAGACAATTTACGTTGCAATCCAAACCTTCGTCTATTCTTGCCTTCTATTTTTCATGATCGGGTACAATTTCAAGGTGGAGAAGTTTTTGTACTTCTACTACTTCATCTTCATGTGCTTTACCTACTTCTCTATGTACGGAATGATGGTTGTTGCCCTGACTCCTGGCCATCAAATTGCTGCAATTGTTATGTCGTTCTTCTTGAGTTTCTGGAACTTGTTCTCGGGCTTCCTCATTCCCAGGCCGCTAATTCCCATCTGGTGGAGGTGGTACTACTGGGGTTCTCCCGTTGCCTGGACAATCTATGGTATCTTCACATCTCAAGTTGGTGACATGAAGACTAACATCGACATTCCTATTCAAGGACCACAAAAAATTGATGTATTTCTCAAAAACTACTTGGGTTTTGACTACGACTTTCTGATCCCTGTCGTCATTGCTCATCTTGGTTGGGTCCTCCTCTTCTTCTTCGTCTTTGCCTACGGCATCAAGTACCTTAACTTCCAAAGGAGATAA

Protein Analysis

1449

Amino Acids

164.34

Weight (kDa)

8.43

Isoelectric Point (pI)

38.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_trans_N PF14510 111 - 159 6.8e-09 ABC-transporter N-terminal
ABC_tran PF00005 185 - 366 3.4e-13 ABC transporter
ABC2_membrane_7 PF19055 400 - 452 2.4e-06 ABC-2 type transporter
ABC2_membrane PF01061 521 - 733 4.5e-45 ABC-2 type transporter
PDR_assoc PF08370 738 - 802 3.6e-23 Plant PDR ABC transporter associated
ABC_tran PF00005 875 - 1027 4.8e-20 ABC transporter
ABC2_membrane PF01061 1172 - 1383 5e-54 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000245)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66950 AT2G36380
fragaria_vesca FvH4_3g29700 FvH4_3g29700 FvH4_3g29700 FvH4_3g29700 FvH4_3g29710 FvH4_3g29740 FvH4_3g29740 FvH4_3g29740 FvH4_3g29740 FvH4_3g29740 FvH4_3g29760 FvH4_3g29781 FvH4_6g18150 FvH4_6g18150 FvH4_6g18150 FvH4_6g18150 FvH4_6g18150
malus_domestica MD02G1254900.v1.1 MD03G1119400.v1.1 MD03G1136800.v1.1 MD03G1137000.v1.1 MD04G1187500.v1.1 MD08G1208900.v1.1 MD08G1209000.v1.1 MD11G1159000.v1.1 MD11G1159100.v1.1 MD11G1159200.v1.1 MD12G1020800.v1.1 MD12G1021000.v1.1 MD14G1017900.v1.1 MD14G1018100.v1.1
prunus_persica Prupe.2G084300_v2.0.a1 Prupe.2G084300_v2.0.a1 Prupe.6G121200_v2.0.a1 Prupe.6G121200_v2.0.a1 Prupe.6G121500_v2.0.a1 Prupe.6G121800_v2.0.a1 Prupe.6G121900_v2.0.a1 Prupe.6G122100_v2.0.a1 Prupe.7G115200_v2.0.a1 Prupe.7G115200_v2.0.a1
pyrus_communis pycom02g21460 pycom03g09270 pycom03g09290 pycom07g05130 pycom08g18050 pycom11g12950 pycom11g12960 pycom11g12970 pycom12g01950 pycom12g01980 pycom14g01620 pycom14g01710 pycom14g01720 pycom16g12190
rosa_chinensis RchiOBHm_Chr1g0340571 RchiOBHm_Chr2g0107751 RchiOBHm_Chr3g0472451 RchiOBHm_Chr5g0055051 RchiOBHm_Chr5g0055091 RchiOBHm_Chr5g0055111 RchiOBHm_Chr5g0055121 RchiOBHm_Chr5g0055131 RchiOBHm_Chr5g0062371 RchiOBHm_Chr6g0265121 RchiOBHm_Chr6g0275771 RchiOBHm_Chr7g0187231
rosa_laevigata RLG00000006319 RLG00000013820 RLG00000024078 RLG00000034960 RLG00000034963 RLG00000034965 RLG00000034966 RLG00000034968 RLG00000034970
rosa_multiflora Rmu_co8358859.1_g000001 Rmu_co8422375.1_g000001 Rmu_co8427767.1_g000001 Rmu_co8474941.1_g000001 Rmu_sc0000925.1_g000009 Rmu_sc0001694.1_g000015 Rmu_sc0001694.1_g000022 Rmu_sc0001694.1_g000024 Rmu_sc0001694.1_g000030 Rmu_sc0003993.1_g000001 Rmu_sc0010632.1_g000001 Rmu_sc0035175.1_g000001
rosa_roxburghii Rroxscaffold_1G00025230 Rroxscaffold_1G00025240 Rroxscaffold_1G00025300 Rroxscaffold_1G00025320 Rroxscaffold_1G00025330 Rroxscaffold_1G00025360 Rroxscaffold_1G00025440 Rroxscaffold_1G00033590 Rroxscaffold_6G00408900
rosa_rugosa Rorug03G0125500.1 Rorug03G0125600.1 Rorug05G0290600 Rorug05G0291100 Rorug05G0291200 Rorug05G0291300
rosa_samantha Rh1CG035400 Rh1CG243200 Rh2DG410600 Rh3AG177500 Rh3CG193800 Rh3CG200900 Rh3DG171700 Rh5AG043000 Rh5AG360500 Rh5AG360900 Rh5AG361000 Rh5AG361300 Rh5AG441100 Rh5CG393700 Rh5CG393900 Rh5CG394200 Rh5DG247300 Rh5DG385200 Rh5DG385500 Rh5DG385800 Rh6BG272200 Rh6DG204000 Rh7AG084700 Rh7AG178300
rosa_wichuraiana Rw0G022120 Rw3G016410 Rw5G033910 Rw5G033920 Rw5G033940 Rw5G033960 Rw5G033970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2448
AatII GACGTC 1 cut(s) 1364
AccB1I GGYRCC 1 cut(s) 449
AccB7I CCANNNNNTGG 1 cut(s) 4092
AccBSI CCGCTC 2 cut(s) 129, 567
AccI GTMKAC 2 cut(s) 80, 264
AccII CGCG 1 cut(s) 2318
AccIII TCCGGA 1 cut(s) 800
AclWI GGATC 4 cut(s) 878, 2372, 2439, 4249
AcoI YGGCCR 5 cut(s) 697, 925, 1462, 1947, 3997
AcsI RAATTY 8 cut(s) 418, 475, 704, 713, 1718, 1875, 2479, 3406
AcuI CTGAAG 2 cut(s) 276, 2445
AcvI CACGTG 1 cut(s) 1277
AcyI GRCGYC 1 cut(s) 1361
AfiI CCNNNNNNNGG 8 cut(s) 183, 496, 617, 3080, 3464, 4092, 4125, 4193
AflII CTTAAG 2 cut(s) 317, 1675
AflIII ACRYGT 1 cut(s) 1042
AgeI ACCGGT 1 cut(s) 2729
AhlI ACTAGT 3 cut(s) 83, 502, 935
AjuI GAANNNNNNNTTGG 4 cut(s) 1050, 1082, 2232, 2264
AloI GAACNNNNNNTCC 6 cut(s) 375, 407, 1814, 1846, 2319, 2351
Alw21I GWGCWC 5 cut(s) 165, 460, 1563, 1732, 2221
AlwI GGATC 4 cut(s) 878, 2372, 2439, 4249
Ama87I CYCGRG 2 cut(s) 2793, 4054
Aor13HI TCCGGA 1 cut(s) 800
AoxI GGCC 8 cut(s) 171, 228, 697, 925, 1462, 1947, 3997, 4073
ApoI RAATTY 8 cut(s) 418, 475, 704, 713, 1718, 1875, 2479, 3406
AsiGI ACCGGT 1 cut(s) 2729
Asp700I GAANNNNTTC 2 cut(s) 3793, 4049
AspLEI GCGC 2 cut(s) 286, 2320
AspS9I GGNCC 8 cut(s) 171, 605, 1070, 1271, 1372, 3245, 4196, 4285
AsuC2I CCSGG 1 cut(s) 2982
AsuHPI GGTGA 8 cut(s) 779, 1013, 1438, 1540, 2148, 2823, 3158, 4172
AsuII TTCGAA 2 cut(s) 1299, 2051
AsuNHI GCTAGC 1 cut(s) 3716
AvaI CYCGRG 2 cut(s) 2793, 4054
AvaII GGWCC 7 cut(s) 605, 1070, 1271, 1372, 3245, 4196, 4285
AxyI CCTNAGG 1 cut(s) 396
BalI TGGCCA 4 cut(s) 699, 927, 1464, 3999
BanI GGYRCC 1 cut(s) 449
BanII GRGCYC 2 cut(s) 165, 1563
BarI GAAGNNNNNNTAC 3 cut(s) 3905, 3937, 4316
BbrPI CACGTG 1 cut(s) 1277
BbsI GAAGAC 3 cut(s) 2424, 3757, 4175
Bbv12I GWGCWC 5 cut(s) 165, 460, 1563, 1732, 2221
BceAI ACGGC 2 cut(s) 2113, 4330
BcgI CGANNNNNNTGC 2 cut(s) 1692, 1726
BciVI GTATCC 2 cut(s) 1435, 3130
BclI TGATCA 2 cut(s) 942, 3669
BcnI CCSGG 1 cut(s) 2982
BcuI ACTAGT 3 cut(s) 83, 502, 935
BfaI CTAG 9 cut(s) 30, 84, 503, 852, 936, 3093, 3174, 3251, 3717
BfmI CTRYAG 2 cut(s) 1408, 3030
BfrI CTTAAG 2 cut(s) 317, 1675
BfuI GTATCC 2 cut(s) 1435, 3130
BglI GCCNNNNNGGC 1 cut(s) 3368
BglII AGATCT 1 cut(s) 664
Bme18I GGWCC 7 cut(s) 605, 1070, 1271, 1372, 3245, 4196, 4285
BmeT110I CYCGRG 2 cut(s) 2793, 4054
BmgT120I GGNCC 8 cut(s) 171, 605, 1070, 1271, 1372, 3245, 4196, 4285
BmiI GGNNCC 6 cut(s) 393, 451, 823, 2065, 3469, 4286
BmrI ACTGGG 2 cut(s) 687, 4117
BmsI GCATC 6 cut(s) 195, 207, 427, 1813, 3079, 4326
BmtI GCTAGC 1 cut(s) 3720
BmuI ACTGGG 2 cut(s) 687, 4117
BpiI GAAGAC 3 cut(s) 2424, 3757, 4175
BplI GAGNNNNNCTC 2 cut(s) 3338, 3370
BpmI CTGGAG 3 cut(s) 1074, 3454, 3577
Bpu10I CCTNAGC 1 cut(s) 3819
Bpu14I TTCGAA 2 cut(s) 1299, 2051
BpuEI CTTGAG 7 cut(s) 149, 515, 944, 1310, 2469, 4054, 4137
BpuMI CCSGG 1 cut(s) 2982
BsaAI YACGTR 2 cut(s) 1277, 1621
BsaBI GATNNNNATC 2 cut(s) 663, 1643
BsaHI GRCGYC 1 cut(s) 1361
BsaWI WCCGGW 4 cut(s) 109, 800, 1025, 2729
BsaXI ACNNNNNCTCC 8 cut(s) 152, 182, 2088, 2118, 2282, 2312, 3216, 3246
Bsc4I CCNNNNNNNGG 8 cut(s) 183, 496, 617, 3080, 3464, 4092, 4125, 4193
Bse118I RCCGGY 3 cut(s) 63, 2729, 3242
Bse21I CCTNAGG 1 cut(s) 396
Bse3DI GCAATG 5 cut(s) 244, 1788, 2019, 3727, 4266
Bse8I GATNNNNATC 2 cut(s) 663, 1643
BseAI TCCGGA 1 cut(s) 800
BseJI GATNNNNATC 2 cut(s) 663, 1643
BseLI CCNNNNNNNGG 8 cut(s) 183, 496, 617, 3080, 3464, 4092, 4125, 4193
BseMI GCAATG 5 cut(s) 244, 1788, 2019, 3727, 4266
BseMII CTCAG 9 cut(s) 212, 373, 737, 1200, 1239, 2255, 2994, 3386, 3833
BseRI GAGGAG 5 cut(s) 167, 170, 173, 3237, 4280
BseYI CCCAGC 1 cut(s) 1842
BsgI GTGCAG 1 cut(s) 1188
Bsh1236I CGCG 1 cut(s) 2318
Bsh1285I CGRYCG 1 cut(s) 80
BshFI GGCC 8 cut(s) 173, 230, 699, 927, 1464, 1949, 3999, 4075
BshNI GGYRCC 1 cut(s) 449
BshTI ACCGGT 1 cut(s) 2729
BsiEI CGRYCG 1 cut(s) 80
BsiHKAI GWGCWC 5 cut(s) 165, 460, 1563, 1732, 2221
BsiHKCI CYCGRG 2 cut(s) 2793, 4054
BslFI GGGAC 1 cut(s) 513
BslI CCNNNNNNNGG 8 cut(s) 183, 496, 617, 3080, 3464, 4092, 4125, 4193
BsmBI CGTCTC 1 cut(s) 1205
BsmFI GGGAC 1 cut(s) 513
BsmI GAATGC 2 cut(s) 899, 1442
BsnI GGCC 8 cut(s) 173, 230, 699, 927, 1464, 1949, 3999, 4075
BsoBI CYCGRG 2 cut(s) 2793, 4054
Bsp119I TTCGAA 2 cut(s) 1299, 2051
Bsp1286I GDGCHC 5 cut(s) 165, 460, 1563, 1732, 2221
Bsp13I TCCGGA 1 cut(s) 800
Bsp1407I TGTACA 1 cut(s) 3158
Bsp19I CCATGG 1 cut(s) 1267
BspANI GGCC 8 cut(s) 173, 230, 699, 927, 1464, 1949, 3999, 4075
BspCNI CTCAG 9 cut(s) 213, 372, 736, 1201, 1240, 2256, 2995, 3385, 3832
BspEI TCCGGA 1 cut(s) 800
BspFNI CGCG 1 cut(s) 2318
BspHI TCATGA 4 cut(s) 903, 1146, 3591, 3885
BspLI GGNNCC 6 cut(s) 393, 451, 823, 2065, 3469, 4286
BspOI GCTAGC 1 cut(s) 3720
BspPI GGATC 4 cut(s) 878, 2372, 2439, 4249
BspQI GCTCTTC 4 cut(s) 1568, 1693, 2327, 2582
BspT104I TTCGAA 2 cut(s) 1299, 2051
BspT107I GGYRCC 1 cut(s) 449
BspTI CTTAAG 2 cut(s) 317, 1675
BsrBI CCGCTC 2 cut(s) 129, 567
BsrDI GCAATG 5 cut(s) 244, 1788, 2019, 3727, 4266
BsrFI RCCGGY 3 cut(s) 63, 2729, 3242
BsrGI TGTACA 1 cut(s) 3158
BssAI RCCGGY 3 cut(s) 63, 2729, 3242
BssNI GRCGYC 1 cut(s) 1361
BssT1I CCWWGG 5 cut(s) 1267, 2029, 2092, 2593, 3709
Bst6I CTCTTC 8 cut(s) 1568, 1693, 2327, 2345, 2582, 2598, 3710, 4298
BstACI GRCGYC 1 cut(s) 1361
BstAFI CTTAAG 2 cut(s) 317, 1675
BstAPI GCANNNNNTGC 3 cut(s) 1577, 1778, 3528
BstAUI TGTACA 1 cut(s) 3158
BstBAI YACGTR 2 cut(s) 1277, 1621
BstBI TTCGAA 2 cut(s) 1299, 2051
BstDSI CCRYGG 3 cut(s) 1267, 2129, 3132
BstEII GGTNACC 1 cut(s) 2765
BstENI CCTNNNNNAGG 1 cut(s) 4191
BstFNI CGCG 1 cut(s) 2318
BstHHI GCGC 2 cut(s) 286, 2320
BstMCI CGRYCG 1 cut(s) 80
BstNSI RCATGY 2 cut(s) 1575, 3531
BstPI GGTNACC 1 cut(s) 2765
BstSFI CTRYAG 2 cut(s) 1408, 3030
BstSNI TACGTA 1 cut(s) 1621
BstUI CGCG 1 cut(s) 2318
BstV2I GAAGAC 3 cut(s) 2424, 3757, 4175
BstX2I RGATCY 2 cut(s) 664, 2377
BstXI CCANNNNNNTGG 2 cut(s) 919, 3336
BstYI RGATCY 2 cut(s) 664, 2377
Bsu36I CCTNAGG 1 cut(s) 396
BsuI GTATCC 2 cut(s) 1435, 3130
BsuRI GGCC 8 cut(s) 173, 230, 699, 927, 1464, 1949, 3999, 4075
BtgI CCRYGG 3 cut(s) 1267, 2129, 3132
BtsI GCAGTG 2 cut(s) 593, 629
BtsIMutI CAGTG 9 cut(s) 46, 593, 629, 1730, 1886, 2363, 2641, 2686, 3544
CciI TCATGA 4 cut(s) 903, 1146, 3591, 3885
CfoI GCGC 2 cut(s) 286, 2320
Cfr10I RCCGGY 3 cut(s) 63, 2729, 3242
Cfr13I GGNCC 8 cut(s) 171, 605, 1070, 1271, 1372, 3245, 4196, 4285
CseI GACGC 3 cut(s) 905, 2142, 2867
CsiI ACCWGGT 2 cut(s) 824, 2650
CspAI ACCGGT 1 cut(s) 2729
DraI TTTAAA 1 cut(s) 1813
DrdI GACNNNNNNGTC 1 cut(s) 2448
DseDI GACNNNNNNGTC 1 cut(s) 2448
EaeI YGGCCR 5 cut(s) 697, 925, 1462, 1947, 3997
Eam1104I CTCTTC 8 cut(s) 1568, 1693, 2327, 2345, 2582, 2598, 3710, 4298
EarI CTCTTC 8 cut(s) 1568, 1693, 2327, 2345, 2582, 2598, 3710, 4298
EciI GGCGGA 1 cut(s) 82
Ecl136II GAGCTC 2 cut(s) 163, 1561
Eco105I TACGTA 1 cut(s) 1621
Eco130I CCWWGG 5 cut(s) 1267, 2029, 2092, 2593, 3709
Eco24I GRGCYC 2 cut(s) 165, 1563
Eco32I GATATC 1 cut(s) 979
Eco47I GGWCC 7 cut(s) 605, 1070, 1271, 1372, 3245, 4196, 4285
Eco53kI GAGCTC 2 cut(s) 163, 1561
Eco57I CTGAAG 2 cut(s) 276, 2445
Eco72I CACGTG 1 cut(s) 1277
Eco81I CCTNAGG 1 cut(s) 396
Eco88I CYCGRG 2 cut(s) 2793, 4054
Eco91I GGTNACC 1 cut(s) 2765
EcoICRI GAGCTC 2 cut(s) 163, 1561
EcoNI CCTNNNNNAGG 1 cut(s) 4191
EcoO109I RGGNCCY 1 cut(s) 4285
EcoO65I GGTNACC 1 cut(s) 2765
EcoRI GAATTC 3 cut(s) 475, 704, 1875
EcoRV GATATC 1 cut(s) 979
EcoT14I CCWWGG 5 cut(s) 1267, 2029, 2092, 2593, 3709
EcoT22I ATGCAT 1 cut(s) 3814
EcoT38I GRGCYC 2 cut(s) 165, 1563
ErhI CCWWGG 5 cut(s) 1267, 2029, 2092, 2593, 3709
Esp3I CGTCTC 1 cut(s) 1205
FalI AAGNNNNNCTT 8 cut(s) 1477, 1509, 1559, 1591, 2684, 2716, 3515, 3547
FaqI GGGAC 1 cut(s) 513
FauI CCCGC 1 cut(s) 122
FbaI TGATCA 2 cut(s) 942, 3669
FblI GTMKAC 2 cut(s) 80, 264
FriOI GRGCYC 2 cut(s) 165, 1563
FspBI CTAG 9 cut(s) 30, 84, 503, 852, 936, 3093, 3174, 3251, 3717
GlaI GCGC 2 cut(s) 285, 2319
GsaI CCCAGC 1 cut(s) 1846
GsuI CTGGAG 3 cut(s) 1074, 3454, 3577
HaeIII GGCC 8 cut(s) 173, 230, 699, 927, 1464, 1949, 3999, 4075
HgaI GACGC 3 cut(s) 905, 2142, 2867
HhaI GCGC 2 cut(s) 286, 2320
Hin1I GRCGYC 1 cut(s) 1361
Hin6I GCGC 2 cut(s) 284, 2318
HinP1I GCGC 2 cut(s) 284, 2318
HincII GTYRAC 2 cut(s) 81, 265
HindII GTYRAC 2 cut(s) 81, 265
HindIII AAGCTT 2 cut(s) 3192, 3505
HphI GGTGA 8 cut(s) 779, 1013, 1438, 1540, 2148, 2823, 3158, 4172
Hpy166II GTNNAC 9 cut(s) 81, 265, 1031, 1264, 1274, 1618, 2283, 2813, 3385
Hpy8I GTNNAC 9 cut(s) 81, 265, 1031, 1264, 1274, 1618, 2283, 2813, 3385
Hpy99I CGWCG 5 cut(s) 269, 899, 2108, 2111, 2445
Hsp92I GRCGYC 1 cut(s) 1361
HspAI GCGC 2 cut(s) 284, 2318
Kpn2I TCCGGA 1 cut(s) 800
KroI GCCGGC 1 cut(s) 63
KroNI GCCGGC 1 cut(s) 65
Ksp22I TGATCA 2 cut(s) 942, 3669
LguI GCTCTTC 4 cut(s) 1568, 1693, 2327, 2582
LmnI GCTCC 9 cut(s) 69, 126, 160, 1558, 1727, 2224, 3473, 3683, 3713
LweI GCATC 6 cut(s) 195, 207, 427, 1813, 3079, 4326
MabI ACCWGGT 2 cut(s) 824, 2650
MaeI CTAG 9 cut(s) 30, 84, 503, 852, 936, 3093, 3174, 3251, 3717
MbiI CCGCTC 2 cut(s) 129, 567
MfeI CAATTG 4 cut(s) 1656, 2058, 2181, 4014
MflI RGATCY 2 cut(s) 664, 2377
MhlI GDGCHC 5 cut(s) 165, 460, 1563, 1732, 2221
MlsI TGGCCA 4 cut(s) 699, 927, 1464, 3999
MluNI TGGCCA 4 cut(s) 699, 927, 1464, 3999
MlyI GAGTC 6 cut(s) 513, 2414, 2602, 2607, 2861, 3985
MmeI TCCRAC 9 cut(s) 361, 487, 488, 799, 1048, 2230, 2641, 2953, 3324
Mox20I TGGCCA 4 cut(s) 699, 927, 1464, 3999
Mph1103I ATGCAT 1 cut(s) 3814
MroI TCCGGA 1 cut(s) 800
MroNI GCCGGC 1 cut(s) 63
MroXI GAANNNNTTC 2 cut(s) 3793, 4049
MscI TGGCCA 4 cut(s) 699, 927, 1464, 3999
MslI CAYNNNNRTG 5 cut(s) 765, 917, 3062, 3110, 3941
Msp20I TGGCCA 4 cut(s) 699, 927, 1464, 3999
MspA1I CMGCKG 3 cut(s) 124, 1946, 2802
MspCI CTTAAG 2 cut(s) 317, 1675
MunI CAATTG 4 cut(s) 1656, 2058, 2181, 4014
Mva1269I GAATGC 2 cut(s) 899, 1442
MvnI CGCG 1 cut(s) 2318
NaeI GCCGGC 1 cut(s) 65
NciI CCSGG 1 cut(s) 2982
NcoI CCATGG 1 cut(s) 1267
NgoMIV GCCGGC 1 cut(s) 63
NheI GCTAGC 1 cut(s) 3716
NlaIV GGNNCC 6 cut(s) 393, 451, 823, 2065, 3469, 4286
NmeAIII GCCGAG 1 cut(s) 3335
NmuCI GTSAC 5 cut(s) 685, 1357, 1528, 2154, 4160
NsiI ATGCAT 1 cut(s) 3814
NspI RCATGY 2 cut(s) 1575, 3531
NspV TTCGAA 2 cut(s) 1299, 2051
PaeI GCATGC 2 cut(s) 1575, 3531
PagI TCATGA 4 cut(s) 903, 1146, 3591, 3885
PciSI GCTCTTC 4 cut(s) 1568, 1693, 2327, 2582
PctI GAATGC 2 cut(s) 899, 1442
PdiI GCCGGC 1 cut(s) 65
PdmI GAANNNNTTC 2 cut(s) 3793, 4049
PfeI GAWTC 6 cut(s) 274, 411, 866, 1709, 1798, 1895
PflMI CCANNNNNTGG 1 cut(s) 4092
PfoI TCCNGGA 1 cut(s) 2980
PinAI ACCGGT 1 cut(s) 2729
PleI GAGTC 6 cut(s) 513, 2414, 2602, 2607, 2860, 3985
PmaCI CACGTG 1 cut(s) 1277
PmlI CACGTG 1 cut(s) 1277
PpsI GAGTC 6 cut(s) 513, 2414, 2602, 2607, 2860, 3985
Ppu21I YACGTR 2 cut(s) 1277, 1621
PpuMI RGGWCCY 1 cut(s) 4285
Psp124BI GAGCTC 2 cut(s) 165, 1563
Psp5II RGGWCCY 1 cut(s) 4285
PspCI CACGTG 1 cut(s) 1277
PspEI GGTNACC 1 cut(s) 2765
PspFI CCCAGC 1 cut(s) 1842
PspN4I GGNNCC 6 cut(s) 393, 451, 823, 2065, 3469, 4286
PspPI GGNCC 8 cut(s) 171, 605, 1070, 1271, 1372, 3245, 4196, 4285
PspPPI RGGWCCY 1 cut(s) 4285
PsrI GAACNNNNNNTAC 4 cut(s) 1391, 1423, 3554, 3586
PsuI RGATCY 2 cut(s) 664, 2377
RseI CAYNNNNRTG 5 cut(s) 765, 917, 3062, 3110, 3941
SacI GAGCTC 2 cut(s) 165, 1563
SalI GTCGAC 2 cut(s) 79, 263
SapI GCTCTTC 4 cut(s) 1568, 1693, 2327, 2582
Sau96I GGNCC 8 cut(s) 171, 605, 1070, 1271, 1372, 3245, 4196, 4285
SchI GAGTC 6 cut(s) 513, 2414, 2602, 2607, 2861, 3985
SduI GDGCHC 5 cut(s) 165, 460, 1563, 1732, 2221
SexAI ACCWGGT 2 cut(s) 824, 2650
SfaNI GCATC 6 cut(s) 195, 207, 427, 1813, 3079, 4326
SfcI CTRYAG 2 cut(s) 1408, 3030
SfuI TTCGAA 2 cut(s) 1299, 2051
SinI GGWCC 7 cut(s) 605, 1070, 1271, 1372, 3245, 4196, 4285
SmiMI CAYNNNNRTG 5 cut(s) 765, 917, 3062, 3110, 3941
SmlI CTYRAG 9 cut(s) 164, 317, 494, 959, 1289, 1675, 2484, 4033, 4152
SmoI CTYRAG 9 cut(s) 164, 317, 494, 959, 1289, 1675, 2484, 4033, 4152
SnaBI TACGTA 1 cut(s) 1621
SpeI ACTAGT 3 cut(s) 83, 502, 935
SphI GCATGC 2 cut(s) 1575, 3531
SspI AATATT 2 cut(s) 2347, 3399
SspMI CTAG 9 cut(s) 30, 84, 503, 852, 936, 3093, 3174, 3251, 3717
SstI GAGCTC 2 cut(s) 165, 1563
StyI CCWWGG 5 cut(s) 1267, 2029, 2092, 2593, 3709
TaqII GACCGA 1 cut(s) 2915
TatI WGTACW 2 cut(s) 3158, 3921
TauI GCSGC 7 cut(s) 8, 136, 176, 1949, 2318, 3104, 4078
TfiI GAWTC 6 cut(s) 274, 411, 866, 1709, 1798, 1895
TscAI CASTG 9 cut(s) 46, 600, 636, 1737, 1893, 2370, 2641, 2686, 3551
TseFI GTSAC 5 cut(s) 685, 1357, 1528, 2154, 4160
Tsp45I GTSAC 5 cut(s) 685, 1357, 1528, 2154, 4160
TspGWI ACGGA 5 cut(s) 1547, 1779, 2003, 2303, 3984
TspRI CASTG 9 cut(s) 46, 600, 636, 1737, 1893, 2370, 2641, 2686, 3551
Van91I CCANNNNNTGG 1 cut(s) 4092
Vha464I CTTAAG 2 cut(s) 317, 1675
VpaK11BI GGWCC 7 cut(s) 605, 1070, 1271, 1372, 3245, 4196, 4285
XagI CCTNNNNNAGG 1 cut(s) 4191
XapI RAATTY 8 cut(s) 418, 475, 704, 713, 1718, 1875, 2479, 3406
XbaI TCTAGA 1 cut(s) 851
XceI RCATGY 2 cut(s) 1575, 3531
XcmI CCANNNNNNNNNTGG 3 cut(s) 1888, 2509, 3061
XmiI GTMKAC 2 cut(s) 80, 264
XmnI GAANNNNTTC 2 cut(s) 3793, 4049
XspI CTAG 9 cut(s) 30, 84, 503, 852, 936, 3093, 3174, 3251, 3717
ZraI GACGTC 1 cut(s) 1362
Zsp2I ATGCAT 1 cut(s) 3814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.