RLG00000024078
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000245
Physical Location & Seq
Forward (+)
31534531 .. 31543108
8578 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024078

Sequence Viewer

Length: 4464 bp
ATGAGTACAGTAACTAATAGGCTCAGCGGCGCCGCGAACCTTTTTCCGGCCGAGGGCAAAGTGCCTATTGTCCAACGCAGTGACCTGAGAACACCAACCAGTTTCGATTACTTTATACCCTCCATTTTAACACATACTTATCCTTACGATATATTCTTTTCGATCTGGAGGCTTACATTTAGGGACTATTGCTGCAAGTTTTCAACGACATCCTTTGAACAAATCACAATGGCGTTGGCTTTGGCCGGAGACGATCTGGCCCGGTCGACGAGCAGCCGGAGAAGCTATGGTTCACATGGGAGCAAGCGGAGCTGGGCGTCCACAAGTTTCCGGGAGGTGTGGCAGGTCCCGCCGGACGTGTTCAACCGGAGTGGCCGGCAGGACGAGGATGAAGAGCTCCGGTGGGCCGCTATCGAGCGGCTTCCGACTTACGACAGACTTAGAAGAGGGATGCTTCGGCAGGTACTCGACAATGGACGAGTGGTTACTGATGAAGTGGATGTTACGAGGCTTGGAGTGCAAGACAAGAAGGTGTTGATGGAGAACATACTCAAAGTTGTGGAAGATGATAATGAAAGGTTTCTAAGGAGACTTAGAGACAGAACTGACAGAGTTGGGATTGAGATTCCGAAGATTGAAGTTAGATATGAGCATCTGTCGATTGAAGGAGATGTGTATGTTGGGAAAAGAGCACTGCCTACTCTGCTTAATGCCACCTTGAACACTATAGAGAGTGTTCTGGGTTTGATTCGTCTTGCTCCATCAAAGAAGAGAAAGATCCAGATACTTAAAGATGTTAATGGAATAGTCAAACCCTCTAGGATGACCCTACTTTTGGGTCCTCCAGGTGCAGGTAAAACAACATTGCTGTTGGCACTTGCAGGGAAGCTTGATGACGATCTAAGGGTATCTGGGAACATCACTTACTGTGGTCATGAATTGAGAGAGTTTGTTCCCCGAAGGACATGTGCTTATATCAGTCAACATGATCTTCACTATGGAGAAATGACAGTGAGGGAGACATTGGATTTTTCGGGACGATGCTTAGGTGTGGGGACTAGATATGAAATGCTGGCTGAACTTTCCAGAAGAGAAAAAGAAGCAGGAATCAAGCCAGATCCTGAGATTGATGCATTCATGAAAGCCACCTCAGTTTCAGGCCAGAAGACTAGTTTGGTCACAGATTATGTTATCAAGATTCTTGGTTTGGATATCTGTTCTGATATTATGGTTGGTGATGAAATGAGAAGGGGTATTTCTGGTGGCCAGAAAAAGCGTGTCACAACTGGGGAAATGTTGGTAGGACCAGCAAAGGTGCTTTTGATGGATGAAATTTCAACAGGGTTAGACAGTTCCACAACTTTTCAGATATGCAGGTACATGAGGCAAATGGTACATATAATGGATGTCACAATGGTCATCTCTCTTCTTCAACCAGCACCAGAGACATTTGAGCTGTTTGATGACCTTATCTTACTTTCAGAAGGTCAAATTGTGTACCAAGGTGCGCGTGAGAGTGTTCTTGAGTTCTTTGAATACATGGGGTTCAAATGCCCGGAGAGGAAAGGAGTTGCTGACTTTTTGCAAGAAGTAACATCTAAGAAGGATCAAGAACAATACTGGTTCAAGAAAAGCGAACCTTACAGATATATTGAAGTTGCTGAGTTTGTTGAGTGCTTCAACAATTTTCAATATGGCCAACAGATTGCATCGGAACTTGGGGTTCCTTATGAAAAGTCAAGAGCTCACCCAGCTGCATTAGTCAAAGAGAAATATGGTATTTCAAATTGGGAACTGTTCAAGGCTTGTTTTGCAAGGGAGTGGCTGCTAATGAAGCGCAATTCTTTTGTGTATGTATTCAAGACAACCCAGATAACAATCATGTCACTAATTGCGTTCACAGTGTTCCTTAGGACTCAAATGCCAGTGGGAACTGTACAAGATGGAGGAAAATTTTTCGGAGCGCTGTTTTTCAGTCTGATTAATGTCATGTTCAATGGAATGGCAGAACTGGCAATGACTGTTTTCAGGCTTCCTGTGTTTTATAAACAGAGGGATTTCTTGTTTTATCCTGCTTGGGCTTTTGGTTTGCCCATTTGGGTCCTTAGGATCCCCCTATCTTTAATGGAGTCGGCAATATGGATTGTTCTTACTTATTACACCATTGGATTTGCTCCAGCTGCTAGCAGATTTTTCAGACAGTTTTTGGCTTTTTTTGGCATACATCAAATGGCACTGTCCCTCTTTCGGTTCATTGCTGCTCTTGGAAGAACACAAACGGTTGCAAATACTCTCGGTACCTTCAGCTTGCTTATGGTGTTTGTTCTGGGAGGATTTATTGTTGCCAAAAATCCAAGAATTAATGAACCTACAGTTGGGAAAGTCCTCCTTAAGTCCAGAGGATTCTTCACAGATGAATATTGGTTCTGGATATGTATTGGAGCACTATTTGGATTTTCTCTTCTCTTTAACATCTTATTCATTGCTGCATTGACTTTTCTGAATCCTCTTGGAGATTCAAAAGCTGTCATTGCTGATGAAAAAAGTGAGAAAAAGAAAAGAAAGTCATCAACTGAAGGTATTGATTTGGCATTGAGGAGTTCTTCAGAGAATGCACCAACGAAAGGAATGGTTTTGCCCTTCCAACCCCTTTCACTCGCATTTAACCATGTGAACTACTATGTCGATATGCCAGCTGAAATGAAGAGTCAAGGAGTTGATCAAGATCGCCTTCAGCTCCTACGAGATGTCAGTGGTGCTTTTAGACCAGGAGTATTGACAGCACTTGTAGGTGTTAGTGGTGCTGGAAAAACAACTCTTATGGACGTCTTGGCAGGAAGAAAGACAGGTGGTTATATTGAAGGAAGTATTAACATCTCCGGTTATCCAAAGAAACAAGAAACATTTGCTCGTGTTAGTGGTTATTGTGAGCAGAATGACATCCATTCACCTCATGTTACTGTATACGAATCTCTGCTATATTCTGCCTGGCTCCGTCTTTCTTCAAATGTAAAGACCCGAACAAGAAAGATGTTTGTTGAGGAAGTCATGGAGTTGGTTGAGCTTAATCCCATTAGAGATGCTCTAGTTGGCCTTCCGGGAATAGATGGTCTTTCAACAGAACAAAGGAAGAGACTAACCATAGCCGTGGAGCTGGTAGCCAACCCCTCTATTATTTTCATGGATGAACCAACATCTGGACTTGATGCCAGGGCTGCTGCCATCGTTATGCGTACTGTGAGAAACACAGTGGACACTGGAAGAACTGTCGTCTGCACGATTCATCAGCCAAGCATAGATATTTTTGAAGCTTTTGACGAGCTGCTTCTGATGAAACGAGGAGGGCAAGTTATATATGCTGGACCTCTAGGTCGCCAATCTCACAAGCTTGTAGAATATTTTGAAGCTGTTCCTGGGGTCACTAAGATTAAGGATGGTTACAATCCTGCTACATGGATGCTTGAGGTTACTGCTCCTTCAGTTGAGGCACAACTGGATGTGGACTTTGGAGACATTTATGCCAATTCCTCACTTTACCAGAGGAACCAAGAGCTTATCAAAGAGCTTAGTATTCCGGTGCCAGGGTCCAAGGATCTCTACTTCCCAACCAAATACTCCCAACCATTCCCTGTTCAATGCAAAGCTTGTTTCTGGAAAATGCATTGGTCTTATTGGAGAAACCCCCAGTACAACGCCATCCGGTTCTTCATGACAATAGTCATTGGTGGTTTATTTGGTCTTATCTTCTGGAACAAGGGACAACAGACAACCCAACAACAAGATCTGATGAACCTTTTGGGAGCCATGTATGCTGCTGTGCTTTTCCTTGGAGCTACAAATGCTTCTGCGGTGCAAGCAGTTGTTGCCATTGAAAGAACAGTTTTCTATCGTGAAAGAGCGGCTGGGATGTATTCTGAACTGCCTTATGCATTTTCTCAGGTGGCCATAGAGACAATCTATGTTGCAATTCAAACTTTTATCTACACTCTTATCCTCTACTCTATGATTGGATTTGAGTGGAAGATAGCCAAATTCCTCTGGTTCTACTACTACATACTGATGTGCTTCATCTACTTCACAATGTATGGGATGATGGTTGTTGCACTCACTCCAGGCCACCAGATTGCTGCAATTGTTATGTCCTTTTTCCTCAGTTTCTGGAATTTGTTTTCCGGGTTTCTCATTCCAAGACCGCAAATCCCAATATGGTGGAGGTGGTACTACTGGGCTTCACCTGTGGCTTGGACACTATATGGCCTTGTGACCTCTCAAGTGGGTGACAAGGATGCTGATCTTGTGGTACCTGGATATGGCACTACGAAATTGAAGACGTTCCTTAAGGATCAGCTGGCTTTTGATTATGATTTCCTTCCGGCTGTCGCAGCTGCCCATGTTGGCTGGGTCCTCCTTTTCTTCTTTGTGTTTGCATATGGAATCAAGTTCCTCAACTTTCAGAGGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1488

Amino Acids

168.84

Weight (kDa)

8.75

Isoelectric Point (pI)

39.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_trans_N PF14510 189 - 238 1.4e-08 ABC-transporter N-terminal
ABC_tran PF00005 263 - 445 7.7e-17 ABC transporter
ABC2_membrane_7 PF19055 478 - 529 1e-05 ABC-2 type transporter
ABC2_membrane PF01061 599 - 783 1.1e-35 ABC-2 type transporter
PDR_assoc PF08370 784 - 844 1.6e-27 Plant PDR ABC transporter associated
ABC_tran PF00005 914 - 1066 3.6e-21 ABC transporter
ABC2_membrane PF01061 1212 - 1424 5.1e-57 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000245)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66950 AT2G36380
fragaria_vesca FvH4_3g29700 FvH4_3g29700 FvH4_3g29700 FvH4_3g29700 FvH4_3g29710 FvH4_3g29740 FvH4_3g29740 FvH4_3g29740 FvH4_3g29740 FvH4_3g29740 FvH4_3g29760 FvH4_3g29781 FvH4_6g18150 FvH4_6g18150 FvH4_6g18150 FvH4_6g18150 FvH4_6g18150
malus_domestica MD02G1254900.v1.1 MD03G1119400.v1.1 MD03G1136800.v1.1 MD03G1137000.v1.1 MD04G1187500.v1.1 MD08G1208900.v1.1 MD08G1209000.v1.1 MD11G1159000.v1.1 MD11G1159100.v1.1 MD11G1159200.v1.1 MD12G1020800.v1.1 MD12G1021000.v1.1 MD14G1017900.v1.1 MD14G1018100.v1.1
prunus_persica Prupe.2G084300_v2.0.a1 Prupe.2G084300_v2.0.a1 Prupe.6G121200_v2.0.a1 Prupe.6G121200_v2.0.a1 Prupe.6G121500_v2.0.a1 Prupe.6G121800_v2.0.a1 Prupe.6G121900_v2.0.a1 Prupe.6G122100_v2.0.a1 Prupe.7G115200_v2.0.a1 Prupe.7G115200_v2.0.a1
pyrus_communis pycom02g21460 pycom03g09270 pycom03g09290 pycom07g05130 pycom08g18050 pycom11g12950 pycom11g12960 pycom11g12970 pycom12g01950 pycom12g01980 pycom14g01620 pycom14g01710 pycom14g01720 pycom16g12190
rosa_chinensis RchiOBHm_Chr1g0340571 RchiOBHm_Chr2g0107751 RchiOBHm_Chr3g0472451 RchiOBHm_Chr5g0055051 RchiOBHm_Chr5g0055091 RchiOBHm_Chr5g0055111 RchiOBHm_Chr5g0055121 RchiOBHm_Chr5g0055131 RchiOBHm_Chr5g0062371 RchiOBHm_Chr6g0265121 RchiOBHm_Chr6g0275771 RchiOBHm_Chr7g0187231
rosa_laevigata RLG00000006319 RLG00000013820 RLG00000024078 RLG00000034960 RLG00000034963 RLG00000034965 RLG00000034966 RLG00000034968 RLG00000034970
rosa_multiflora Rmu_co8358859.1_g000001 Rmu_co8422375.1_g000001 Rmu_co8427767.1_g000001 Rmu_co8474941.1_g000001 Rmu_sc0000925.1_g000009 Rmu_sc0001694.1_g000015 Rmu_sc0001694.1_g000022 Rmu_sc0001694.1_g000024 Rmu_sc0001694.1_g000030 Rmu_sc0003993.1_g000001 Rmu_sc0010632.1_g000001 Rmu_sc0035175.1_g000001
rosa_roxburghii Rroxscaffold_1G00025230 Rroxscaffold_1G00025240 Rroxscaffold_1G00025300 Rroxscaffold_1G00025320 Rroxscaffold_1G00025330 Rroxscaffold_1G00025360 Rroxscaffold_1G00025440 Rroxscaffold_1G00033590 Rroxscaffold_6G00408900
rosa_rugosa Rorug03G0125500.1 Rorug03G0125600.1 Rorug05G0290600 Rorug05G0291100 Rorug05G0291200 Rorug05G0291300
rosa_samantha Rh1CG035400 Rh1CG243200 Rh2DG410600 Rh3AG177500 Rh3CG193800 Rh3CG200900 Rh3DG171700 Rh5AG043000 Rh5AG360500 Rh5AG360900 Rh5AG361000 Rh5AG361300 Rh5AG441100 Rh5CG393700 Rh5CG393900 Rh5CG394200 Rh5DG247300 Rh5DG385200 Rh5DG385500 Rh5DG385800 Rh6BG272200 Rh6DG204000 Rh7AG084700 Rh7AG178300
rosa_wichuraiana Rw0G022120 Rw3G016410 Rw5G033910 Rw5G033920 Rw5G033940 Rw5G033960 Rw5G033970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2046
AasI GACNNNNNNGTC 1 cut(s) 3369
AatII GACGTC 1 cut(s) 2829
Acc36I ACCTGC 4 cut(s) 334, 451, 842, 1365
Acc65I GGTACC 2 cut(s) 2297, 4300
AccB1I GGYRCC 4 cut(s) 29, 2297, 3577, 4300
AccB7I CCANNNNNTGG 1 cut(s) 3197
AccBSI CCGCTC 2 cut(s) 418, 3899
AccI GTMKAC 2 cut(s) 266, 2964
AccII CGCG 2 cut(s) 35, 1510
AciI CCGC 9 cut(s) 27, 33, 307, 350, 408, 418, 3848, 3899, 4193
AclWI GGATC 7 cut(s) 772, 1112, 1614, 2104, 2117, 3600, 4350
AcoI YGGCCR 6 cut(s) 48, 243, 373, 1264, 1696, 3942
AcsI RAATTY 4 cut(s) 1332, 1952, 4031, 4162
AcuI CTGAAG 5 cut(s) 2287, 2589, 2595, 2717, 3462
AcyI GRCGYC 3 cut(s) 30, 317, 2826
AfeI AGCGCT 1 cut(s) 1965
AflII CTTAAG 2 cut(s) 2390, 4337
AflIII ACRYGT 2 cut(s) 357, 965
AhlI ACTAGT 1 cut(s) 1169
AjiI CACGTC 1 cut(s) 358
AjnI CCWGG 8 cut(s) 844, 2767, 2987, 3209, 3412, 3580, 4111, 4303
AjuI GAANNNNNNNTTGG 4 cut(s) 1157, 1189, 2358, 2390
Alw21I GWGCWC 4 cut(s) 399, 694, 1747, 2446
Alw26I GTCTC 8 cut(s) 243, 583, 591, 1013, 1439, 3127, 3504, 3944
AlwI GGATC 7 cut(s) 772, 1112, 1614, 2104, 2117, 3600, 4350
AlwNI CAGNNNCTG 2 cut(s) 1121, 4158
Aor51HI AGCGCT 1 cut(s) 1965
ApoI RAATTY 4 cut(s) 1332, 1952, 4031, 4162
ArsI GACNNNNNNTTYG 2 cut(s) 817, 849
AseI ATTAAT 2 cut(s) 1983, 2361
Asp700I GAANNNNTTC 3 cut(s) 579, 3408, 4331
Asp718I GGTACC 2 cut(s) 2297, 4300
AspLEI GCGC 4 cut(s) 32, 1510, 1839, 1966
AspS9I GGNCC 9 cut(s) 259, 346, 405, 839, 1304, 2101, 3362, 3585, 4402
AsuC2I CCSGG 5 cut(s) 262, 332, 1556, 3099, 4174
AsuHPI GGTGA 5 cut(s) 1247, 1739, 2940, 4224, 4289
AsuNHI GCTAGC 1 cut(s) 2183
AvaII GGWCC 7 cut(s) 346, 839, 1304, 2101, 3362, 3585, 4402
AxyI CCTNAGG 2 cut(s) 1910, 2105
BalI TGGCCA 3 cut(s) 1266, 1698, 3944
BamHI GGATCC 1 cut(s) 2109
BanI GGYRCC 4 cut(s) 29, 2297, 3577, 4300
BanII GRGCYC 2 cut(s) 399, 1747
BauI CACGAG 1 cut(s) 2910
BbsI GAAGAC 2 cut(s) 1172, 4334
Bbv12I GWGCWC 4 cut(s) 399, 694, 1747, 2446
BccI CCATC 9 cut(s) 532, 769, 1318, 1937, 3101, 3230, 3428, 3704, 4087
BceAI ACGGC 1 cut(s) 3131
BciT130I CCWGG 8 cut(s) 846, 2769, 2989, 3211, 3414, 3582, 4113, 4305
BclI TGATCA 1 cut(s) 2719
BcnI CCSGG 5 cut(s) 262, 332, 1556, 3099, 4174
BcoDI GTCTC 8 cut(s) 243, 583, 591, 1013, 1439, 3127, 3504, 3944
BcuI ACTAGT 1 cut(s) 1169
BfaI CTAG 6 cut(s) 819, 1059, 1170, 2184, 3086, 3368
BfmI CTRYAG 2 cut(s) 726, 2370
BfoI RGCGCY 2 cut(s) 33, 1967
BfrI CTTAAG 2 cut(s) 2390, 4337
BfuAI ACCTGC 4 cut(s) 334, 451, 842, 1365
BglII AGATCT 1 cut(s) 3781
BlpI GCTNAGC 1 cut(s) 23
Bme18I GGWCC 7 cut(s) 346, 839, 1304, 2101, 3362, 3585, 4402
BmgBI CACGTC 1 cut(s) 358
BmgT120I GGNCC 9 cut(s) 259, 346, 405, 839, 1304, 2101, 3362, 3585, 4402
BmrI ACTGGG 3 cut(s) 1296, 3679, 4234
BmsI GCATC 9 cut(s) 441, 661, 1031, 1120, 1718, 3070, 3196, 3447, 4276
BmtI GCTAGC 1 cut(s) 2187
BmuI ACTGGG 3 cut(s) 1296, 3679, 4234
BoxI GACNNNNGTC 1 cut(s) 3716
BpiI GAAGAC 2 cut(s) 1172, 4334
BpmI CTGGAG 4 cut(s) 187, 828, 2160, 4095
Bpu10I CCTNAGC 1 cut(s) 1045
Bpu1102I GCTNAGC 1 cut(s) 23
BpuEI CTTGAG 3 cut(s) 1544, 3482, 4254
BpuMI CCSGG 5 cut(s) 262, 332, 1556, 3099, 4174
BsaBI GATNNNNATC 4 cut(s) 897, 1877, 2724, 4290
BsaHI GRCGYC 3 cut(s) 30, 317, 2826
BsaJI CCNNGG 8 cut(s) 51, 1501, 3147, 3210, 3413, 3581, 3588, 3826
BsaWI WCCGGW 5 cut(s) 366, 399, 2879, 3574, 3699
BsaXI ACNNNNNCTCC 2 cut(s) 3333, 3363
Bse118I RCCGGY 1 cut(s) 375
Bse1I ACTGG 9 cut(s) 99, 1291, 1625, 1925, 2016, 3262, 3498, 3685, 4229
Bse21I CCTNAGG 2 cut(s) 1910, 2105
Bse3DI GCAATG 5 cut(s) 863, 2022, 2253, 2481, 2529
Bse8I GATNNNNATC 4 cut(s) 897, 1877, 2724, 4290
BseBI CCWGG 8 cut(s) 846, 2769, 2989, 3211, 3414, 3582, 4113, 4305
BseDI CCNNGG 8 cut(s) 51, 1501, 3147, 3210, 3413, 3581, 3588, 3826
BseJI GATNNNNATC 4 cut(s) 897, 1877, 2724, 4290
BseMI GCAATG 5 cut(s) 863, 2022, 2253, 2481, 2529
BseMII CTCAG 7 cut(s) 37, 77, 1113, 1164, 1653, 3950, 4165
BseNI ACTGG 9 cut(s) 99, 1291, 1625, 1925, 2016, 3262, 3498, 3685, 4229
BseRI GAGGAG 2 cut(s) 2611, 3354
BseX3I CGGCCG 1 cut(s) 48
BseYI CCCAGC 4 cut(s) 312, 1750, 3902, 4398
BsgI GTGCAG 2 cut(s) 870, 3259
Bsh1236I CGCG 2 cut(s) 35, 1510
Bsh1285I CGRYCG 2 cut(s) 51, 266
BshNI GGYRCC 4 cut(s) 29, 2297, 3577, 4300
BsiEI CGRYCG 2 cut(s) 51, 266
BsiHKAI GWGCWC 4 cut(s) 399, 694, 1747, 2446
BslFI GGGAC 6 cut(s) 197, 332, 1050, 1069, 2224, 3771
BsmAI GTCTC 8 cut(s) 243, 583, 591, 1013, 1439, 3127, 3504, 3944
BsmBI CGTCTC 1 cut(s) 243
BsmFI GGGAC 6 cut(s) 197, 332, 1050, 1069, 2224, 3771
BsmI GAATGC 2 cut(s) 1133, 2617
Bsp1286I GDGCHC 4 cut(s) 399, 694, 1747, 2446
Bsp1407I TGTACA 1 cut(s) 1936
Bsp1720I GCTNAGC 1 cut(s) 23
BspACI CCGC 9 cut(s) 27, 33, 307, 350, 408, 418, 3848, 3899, 4193
BspCNI CTCAG 7 cut(s) 36, 78, 1114, 1163, 1654, 3949, 4164
BspFNI CGCG 2 cut(s) 35, 1510
BspHI TCATGA 3 cut(s) 934, 1137, 3708
BspMI ACCTGC 4 cut(s) 334, 451, 842, 1365
BspOI GCTAGC 1 cut(s) 2187
BspPI GGATC 7 cut(s) 772, 1112, 1614, 2104, 2117, 3600, 4350
BspQI GCTCTTC 1 cut(s) 387
BspT107I GGYRCC 4 cut(s) 29, 2297, 3577, 4300
BspTI CTTAAG 2 cut(s) 2390, 4337
BsrBI CCGCTC 2 cut(s) 418, 3899
BsrDI GCAATG 5 cut(s) 863, 2022, 2253, 2481, 2529
BsrFI RCCGGY 1 cut(s) 375
BsrGI TGTACA 1 cut(s) 1936
BsrI ACTGG 9 cut(s) 99, 1291, 1625, 1925, 2016, 3262, 3498, 3685, 4229
BssAI RCCGGY 1 cut(s) 375
BssECI CCNNGG 8 cut(s) 51, 1501, 3147, 3210, 3413, 3581, 3588, 3826
BssNAI GTATAC 1 cut(s) 2965
BssNI GRCGYC 3 cut(s) 30, 317, 2826
BssSI CACGAG 1 cut(s) 2910
BssT1I CCWWGG 3 cut(s) 1501, 3588, 3826
Bst1107I GTATAC 1 cut(s) 2965
Bst2BI CACGAG 1 cut(s) 2910
Bst2UI CCWGG 8 cut(s) 846, 2769, 2989, 3211, 3414, 3582, 4113, 4305
Bst6I CTCTTC 8 cut(s) 387, 439, 764, 1084, 1431, 2466, 2699, 3125
BstACI GRCGYC 3 cut(s) 30, 317, 2826
BstAFI CTTAAG 2 cut(s) 2390, 4337
BstAPI GCANNNNNTGC 1 cut(s) 3863
BstAUI TGTACA 1 cut(s) 1936
BstC8I GCNNGC 8 cut(s) 305, 377, 1074, 2185, 2309, 2694, 3855, 4350
BstDSI CCRYGG 1 cut(s) 3147
BstFNI CGCG 2 cut(s) 35, 1510
BstH2I RGCGCY 2 cut(s) 33, 1967
BstHHI GCGC 4 cut(s) 32, 1510, 1839, 1966
BstMAI GTCTC 8 cut(s) 243, 583, 591, 1013, 1439, 3127, 3504, 3944
BstMCI CGRYCG 2 cut(s) 51, 266
BstNI CCWGG 8 cut(s) 846, 2769, 2989, 3211, 3414, 3582, 4113, 4305
BstNSI RCATGY 1 cut(s) 969
BstPAI GACNNNNGTC 1 cut(s) 3716
BstSFI CTRYAG 2 cut(s) 726, 2370
BstUI CGCG 2 cut(s) 35, 1510
BstV2I GAAGAC 2 cut(s) 1172, 4334
BstX2I RGATCY 5 cut(s) 777, 1117, 2109, 3592, 3781
BstXI CCANNNNNNTGG 2 cut(s) 3148, 4209
BstYI RGATCY 5 cut(s) 777, 1117, 2109, 3592, 3781
BstZ17I GTATAC 1 cut(s) 2965
BstZI CGGCCG 1 cut(s) 48
Bsu36I CCTNAGG 2 cut(s) 1910, 2105
BtgI CCRYGG 1 cut(s) 3147
BtrI CACGTC 1 cut(s) 358
BtsI GCAGTG 2 cut(s) 85, 692
BtsIMutI CAGTG 9 cut(s) 85, 692, 1017, 1908, 1932, 2234, 2758, 3255, 3255
BveI ACCTGC 4 cut(s) 334, 451, 842, 1365
Cac8I GCNNGC 8 cut(s) 305, 377, 1074, 2185, 2309, 2694, 3855, 4350
CaiI CAGNNNCTG 2 cut(s) 1121, 4158
CciI TCATGA 3 cut(s) 934, 1137, 3708
CfoI GCGC 4 cut(s) 32, 1510, 1839, 1966
Cfr10I RCCGGY 1 cut(s) 375
Cfr13I GGNCC 9 cut(s) 259, 346, 405, 839, 1304, 2101, 3362, 3585, 4402
CseI GACGC 1 cut(s) 306
CspCI CAANNNNNGTGG 2 cut(s) 352, 387
DinI GGCGCC 1 cut(s) 31
DrdI GACNNNNNNGTC 1 cut(s) 3369
DseDI GACNNNNNNGTC 1 cut(s) 3369
EaeI YGGCCR 6 cut(s) 48, 243, 373, 1264, 1696, 3942
EagI CGGCCG 1 cut(s) 48
Eam1104I CTCTTC 8 cut(s) 387, 439, 764, 1084, 1431, 2466, 2699, 3125
EarI CTCTTC 8 cut(s) 387, 439, 764, 1084, 1431, 2466, 2699, 3125
Ecl136II GAGCTC 2 cut(s) 397, 1745
EclXI CGGCCG 1 cut(s) 48
Eco130I CCWWGG 3 cut(s) 1501, 3588, 3826
Eco24I GRGCYC 2 cut(s) 399, 1747
Eco32I GATATC 1 cut(s) 1213
Eco47I GGWCC 7 cut(s) 346, 839, 1304, 2101, 3362, 3585, 4402
Eco47III AGCGCT 1 cut(s) 1965
Eco52I CGGCCG 1 cut(s) 48
Eco53kI GAGCTC 2 cut(s) 397, 1745
Eco57I CTGAAG 5 cut(s) 2287, 2589, 2595, 2717, 3462
Eco81I CCTNAGG 2 cut(s) 1910, 2105
EcoICRI GAGCTC 2 cut(s) 397, 1745
EcoO109I RGGNCCY 4 cut(s) 346, 839, 2101, 4402
EcoRII CCWGG 8 cut(s) 844, 2767, 2987, 3209, 3412, 3580, 4111, 4303
EcoRV GATATC 1 cut(s) 1213
EcoT14I CCWWGG 3 cut(s) 1501, 3588, 3826
EcoT22I ATGCAT 3 cut(s) 1135, 3663, 3931
EcoT38I GRGCYC 2 cut(s) 399, 1747
EgeI GGCGCC 1 cut(s) 31
EheI GGCGCC 1 cut(s) 31
ErhI CCWWGG 3 cut(s) 1501, 3588, 3826
Esp3I CGTCTC 1 cut(s) 243
FalI AAGNNNNNCTT 6 cut(s) 1624, 1656, 2373, 2405, 2715, 2747
FaqI GGGAC 6 cut(s) 197, 332, 1050, 1069, 2224, 3771
FauI CCCGC 1 cut(s) 357
FauNDI CATATG 1 cut(s) 4429
FbaI TGATCA 1 cut(s) 2719
FblI GTMKAC 2 cut(s) 266, 2964
FriOI GRGCYC 2 cut(s) 399, 1747
FspBI CTAG 6 cut(s) 819, 1059, 1170, 2184, 3086, 3368
GlaI GCGC 4 cut(s) 31, 1509, 1838, 1965
GsaI CCCAGC 4 cut(s) 316, 1754, 3906, 4402
GsuI CTGGAG 4 cut(s) 187, 828, 2160, 4095
HaeII RGCGCY 2 cut(s) 33, 1967
HgaI GACGC 1 cut(s) 306
HhaI GCGC 4 cut(s) 32, 1510, 1839, 1966
Hin1I GRCGYC 3 cut(s) 30, 317, 2826
Hin6I GCGC 4 cut(s) 30, 1508, 1837, 1964
HinP1I GCGC 4 cut(s) 30, 1508, 1837, 1964
HincII GTYRAC 2 cut(s) 267, 983
HindII GTYRAC 2 cut(s) 267, 983
HindIII AAGCTT 4 cut(s) 887, 3309, 3386, 3642
HphI GGTGA 5 cut(s) 1247, 1739, 2940, 4224, 4289
Hpy99I CGWCG 1 cut(s) 271
HpyCH4IV ACGT 3 cut(s) 357, 2826, 4331
HpySE526I ACGT 3 cut(s) 357, 2826, 4331
Hsp92I GRCGYC 3 cut(s) 30, 317, 2826
HspAI GCGC 4 cut(s) 30, 1508, 1837, 1964
KasI GGCGCC 1 cut(s) 29
KpnI GGTACC 2 cut(s) 2301, 4304
KroI GCCGGC 1 cut(s) 375
KroNI GCCGGC 1 cut(s) 377
Ksp22I TGATCA 1 cut(s) 2719
LguI GCTCTTC 1 cut(s) 387
LweI GCATC 9 cut(s) 441, 661, 1031, 1120, 1718, 3070, 3196, 3447, 4276
MaeI CTAG 6 cut(s) 819, 1059, 1170, 2184, 3086, 3368
MaeII ACGT 3 cut(s) 357, 2826, 4331
MbiI CCGCTC 2 cut(s) 418, 3899
MfeI CAATTG 1 cut(s) 4131
MflI RGATCY 5 cut(s) 777, 1117, 2109, 3592, 3781
MhlI GDGCHC 4 cut(s) 399, 694, 1747, 2446
MlsI TGGCCA 3 cut(s) 1266, 1698, 3944
MluNI TGGCCA 3 cut(s) 1266, 1698, 3944
Mly113I GGCGCC 1 cut(s) 30
MlyI GAGTC 3 cut(s) 1909, 2138, 2716
MmeI TCCRAC 3 cut(s) 97, 449, 2668
Mox20I TGGCCA 3 cut(s) 1266, 1698, 3944
Mph1103I ATGCAT 3 cut(s) 1135, 3663, 3931
MroNI GCCGGC 1 cut(s) 375
MroXI GAANNNNTTC 3 cut(s) 579, 3408, 4331
MscI TGGCCA 3 cut(s) 1266, 1698, 3944
MslI CAYNNNNRTG 3 cut(s) 3146, 3227, 4058
Msp20I TGGCCA 3 cut(s) 1266, 1698, 3944
MspA1I CMGCKG 6 cut(s) 27, 1754, 2180, 2696, 4348, 4385
MspCI CTTAAG 2 cut(s) 2390, 4337
MunI CAATTG 1 cut(s) 4131
Mva1269I GAATGC 2 cut(s) 1133, 2617
MvaI CCWGG 8 cut(s) 846, 2769, 2989, 3211, 3414, 3582, 4113, 4305
MvnI CGCG 2 cut(s) 35, 1510
NaeI GCCGGC 1 cut(s) 377
NarI GGCGCC 1 cut(s) 30
NciI CCSGG 5 cut(s) 262, 332, 1556, 3099, 4174
NdeI CATATG 1 cut(s) 4429
NgoMIV GCCGGC 1 cut(s) 375
NheI GCTAGC 1 cut(s) 2183
NmeAIII GCCGAG 1 cut(s) 76
NmuCI GTSAC 8 cut(s) 80, 1177, 1279, 1408, 1884, 3418, 4261, 4277
NsiI ATGCAT 3 cut(s) 1135, 3663, 3931
NspI RCATGY 1 cut(s) 969
PagI TCATGA 3 cut(s) 934, 1137, 3708
PciI ACATGT 1 cut(s) 965
PciSI GCTCTTC 1 cut(s) 387
PctI GAATGC 2 cut(s) 1133, 2617
PdiI GCCGGC 1 cut(s) 377
PdmI GAANNNNTTC 3 cut(s) 579, 3408, 4331
PflMI CCANNNNNTGG 1 cut(s) 3197
PfoI TCCNGGA 2 cut(s) 330, 3097
PleI GAGTC 3 cut(s) 1909, 2137, 2715
PluTI GGCGCC 1 cut(s) 33
PpsI GAGTC 3 cut(s) 1909, 2137, 2715
PpuMI RGGWCCY 4 cut(s) 346, 839, 2101, 4402
PscI ACATGT 1 cut(s) 965
PshAI GACNNNNGTC 1 cut(s) 3716
PshBI ATTAAT 2 cut(s) 1983, 2361
PsiI TTATAA 1 cut(s) 2046
Psp124BI GAGCTC 2 cut(s) 399, 1747
Psp5II RGGWCCY 4 cut(s) 346, 839, 2101, 4402
Psp6I CCWGG 8 cut(s) 844, 2767, 2987, 3209, 3412, 3580, 4111, 4303
PspFI CCCAGC 4 cut(s) 312, 1750, 3902, 4398
PspGI CCWGG 8 cut(s) 844, 2767, 2987, 3209, 3412, 3580, 4111, 4303
PspPI GGNCC 9 cut(s) 259, 346, 405, 839, 1304, 2101, 3362, 3585, 4402
PspPPI RGGWCCY 4 cut(s) 346, 839, 2101, 4402
PsrI GAACNNNNNNTAC 2 cut(s) 908, 940
PstNI CAGNNNCTG 2 cut(s) 1121, 4158
PsuI RGATCY 5 cut(s) 777, 1117, 2109, 3592, 3781
PvuII CAGCTG 5 cut(s) 1754, 2180, 2696, 4348, 4385
RseI CAYNNNNRTG 3 cut(s) 3146, 3227, 4058
SacI GAGCTC 2 cut(s) 399, 1747
SalI GTCGAC 1 cut(s) 265
SapI GCTCTTC 1 cut(s) 387
Sau96I GGNCC 9 cut(s) 259, 346, 405, 839, 1304, 2101, 3362, 3585, 4402
SchI GAGTC 3 cut(s) 1909, 2138, 2716
SduI GDGCHC 4 cut(s) 399, 694, 1747, 2446
SfaNI GCATC 9 cut(s) 441, 661, 1031, 1120, 1718, 3070, 3196, 3447, 4276
SfcI CTRYAG 2 cut(s) 726, 2370
SfoI GGCGCC 1 cut(s) 31
SinI GGWCC 7 cut(s) 346, 839, 1304, 2101, 3362, 3585, 4402
SmiMI CAYNNNNRTG 3 cut(s) 3146, 3227, 4058
SmlI CTYRAG 5 cut(s) 1523, 2390, 3461, 4269, 4337
SmoI CTYRAG 5 cut(s) 1523, 2390, 3461, 4269, 4337
SpeI ACTAGT 1 cut(s) 1169
SsiI CCGC 9 cut(s) 27, 33, 307, 350, 408, 418, 3848, 3899, 4193
SspDI GGCGCC 1 cut(s) 29
SspI AATATT 2 cut(s) 2420, 3398
SspMI CTAG 6 cut(s) 819, 1059, 1170, 2184, 3086, 3368
SstI GAGCTC 2 cut(s) 399, 1747
StyI CCWWGG 3 cut(s) 1501, 3588, 3826
TaiI ACGT 3 cut(s) 360, 2829, 4334
TaqI TCGA 7 cut(s) 105, 161, 266, 414, 468, 659, 2685
TatI WGTACW 3 cut(s) 5, 1936, 3687
TauI GCSGC 5 cut(s) 30, 35, 410, 421, 3902
TscAI CASTG 9 cut(s) 85, 699, 1017, 1908, 1932, 2241, 2758, 3255, 3262
TseFI GTSAC 8 cut(s) 80, 1177, 1279, 1408, 1884, 3418, 4261, 4277
Tsp45I GTSAC 8 cut(s) 80, 1177, 1279, 1408, 1884, 3418, 4261, 4277
TspGWI ACGGA 1 cut(s) 2984
TspRI CASTG 9 cut(s) 85, 699, 1017, 1908, 1932, 2241, 2758, 3255, 3262
Van91I CCANNNNNTGG 1 cut(s) 3197
Vha464I CTTAAG 2 cut(s) 2390, 4337
VpaK11BI GGWCC 7 cut(s) 346, 839, 1304, 2101, 3362, 3585, 4402
VspI ATTAAT 2 cut(s) 1983, 2361
XapI RAATTY 4 cut(s) 1332, 1952, 4031, 4162
XceI RCATGY 1 cut(s) 969
XmiI GTMKAC 2 cut(s) 266, 2964
XmnI GAANNNNTTC 3 cut(s) 579, 3408, 4331
XspI CTAG 6 cut(s) 819, 1059, 1170, 2184, 3086, 3368
ZraI GACGTC 1 cut(s) 2827
Zsp2I ATGCAT 3 cut(s) 1135, 3663, 3931
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.