pycom14g01720
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0000245
Physical Location & Seq
Reverse (-)
1250218 .. 1255630
5413 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g01720.4

Sequence Viewer

Length: 3699 bp
ATGATCGATCTAAGAATTTCTGGGAAAATCACATATTGTGGCCATGAACTGAATGAGTTTGTTCCTAAAAGAACATGTGCTTACATTAGTCAGCATGATCTTCACTATGGAGAAATGACAGTGAGAGAGACTTTGGATTTCTCGGGACGATGTTTGGGTGTGGGAACTAGATACCAAATGCTGGCTGATCTCTCAAGAAGAGAGAAAGAAGCCGGAATCAAGCCTGACCCCGAGATTGATGCATTCATGAAAGCCACCTCAGTCTCAGGCCAGAAGACTAGTTTGGTCACAGATTATGTTCTCAAGATACTTGGATTGGATATTTGTGCTGATATCATGGTTGGTGATGATATGAGAAGGGGTATTTCCGGTGGCCAGAAAAAGCGCGTGACCACCGGGGAGATGTTGGTAGGACCGGCAAAGGTTCTTCTGATGGATGAAATATCAACAGGGTTAGACAGTTCCACAACTTTTCAGATATGCAGCTACATGCGGCAATTGGTTCACATAATGGATGTTACCATGGTTATCTCTCTTCTGCAGCCCGCACCGGAGTCATTTGAGCTCTTCGACGACCTAATACTGCTTTCGGAAGGGCAAATCGTGTACCAAGGTCCACGCGAGAGTGTCCTTGAGTTCTTCGAACACGTGGGATTCAAGTGCCCAGAGAGGAAAGGAATAGCTGATTTTTTGCAAGAGGTGACATCTAAGAAGGACCAAGAACAATATTGGTTAAAAAAAAGCCAACCCTACAGATATATCTCGGTTCCTGAGTTCGTGGAGGCCTTCAGCTTTTTTCGGAGTGGCCAGCAACTTGCAGCTGATCTCAGGGTTCCTTACGATAAGTCCAGAGCTCATCCGGCTGCATTGGTCACAGAGAAGTATGGCATTTCAAATTGGGAATTGTTCAAGGCATGTTTTTCGAGGGAGTGGCTGCTAATGAAGCGCAATTCATTCGTGTACATATTCAAGACCACGCAGATTACAATCATGTCTTTGATTGCCTTCACTATGTTCCTTAGAACAGAAATGCCAGTGGGCACAGTACAAAATGGAGGAAAATTTTTAGGAGCGTTGTTCTTCAGTCTAATTAATGTGATGTTTAATGGTATGGCAGAACTGGCAATGACTATTTTCAGACTTCCTGTTTTCTATAAGCAGAGGGATTTCTTGTTTTATCCTGCGTGGGCTTTTGGGTTACCAATTTGGGTCCTCAGGATCCCACTTTCGTTCGTGGAATCAGGGATTTGGATCATTCTTACCTATTACACCATTGGATTTGCTCCATCTGCTAGCAGATTTTTCAAACAGTTCTTGGCATTCTTTGGCATACATCAGATGGCTCTGTCCCTCTTTCGGTTCATTGCTGCACTTGGAAGAACACAAGTTGTTGCTAGCACGCTCGGTACCTTTACCTTGCTAATGGTATTTGTCCTAGGAGGATTTATTGTTGCCAAAAATGACATTGAGCCTTGGATGCTATGGGGCTACTACATTTCTCCTATGATGTACGGACAGAATGCGATTGTCATGAATGAATTCCTTGACAAAAGATGGAGCGCGCCAAATACAGATCCAAGAATTAAATTAACTACAGTCGGGAAAGTCCTTCTCAAGTCTAGAGGATTCTTCACAGATGAATACTGGTTTTGGATATGTATTGGAGCACTATTTGGTTTTTCTCTTCTCTTCAACATCTTGTTCATTGCAGCATTGACTTTTCTGAACCCTCTTGGGGATTCAAAAGCTGTCATAGCTGATGACAAAAGTGAAGGAAAGAGAAGGAAGTCGTTAAGCGAAGAAATGACAGTGAAAAGTTCTTCAGAAATTTCTGGTGCTTCGGATCATACACCAAAGAAAGGAATGGTTTTGCCTTTCCAACCCCTTTCGCTTGCGTTTAACCATGTGAACTACTTTGTGGATATGCCCCATGAAATGAAGACTCATGGAGTTGAAGAAGATCGCCTTCAATTGCTACGAGATGTCAGTGGTGCTTTTAGACCGGGAATATTGACAGCACTTGTAGGTGTTAGTGGTGCTGGAAAGACAACTCTTATGGACGTCCTGGCAGGTAGAAAGACTGGTGGATATATCGAAGGGACCATTAACATATCTGGTTACCCAAAGAAACAAGAAACATTTTCTCGTGTCAGTGGTTATTGTGAACAGAATGACATTCATTCCCCGCATGTTACTGTCTATGAATCTCTCCTGTATTCAGCCTGGCTCCGTCTTGCTTCTGATGTCAATACACAAACACGAAAGATGTTTGTTGAAGAAGTCATGGATTTGGTTGAGCTTAATCCAATAAGAAATTCTCTCGTTGGCCTCCCGGGACTAGATGGTCTTTCAACAGAACAACGGAAGAGACTAACCATAGCTGTGGAGCTGGTTGCTAACCCCTCTATCATTTTCATGGATGAACCGACATCTGGTCTCGATGCCAGGGCTGCTGCTATCGTCATGCGTACTGTGAGAAACACAGTGGACACAGGCAGAACTGTTGTCTGCACAATTCACCAGCCAAGCATAGATATTTTTGAATCTTTCGACGAATTACTCTTAATGAAACGTGGAGGGCAAGTGATTTATGCTGGACCCCTCGGCCGTCACTCTCACAAGCTTGTAGAATATTTTGAAGCCATCCCAGGTGTGCCCAAGATCAAGGATGTTTACAATCCTGCTACATGGATGCTTGAGGTCACTGCTCCTGCAGTTGAGACTCAACTTGATGTGGACTTTGCAGACATTTACCAAAACTCCTCACTTTACCAGAAGAACCAGGAGCTGATCAAAGAGCTTAGTACACCGGCTCCCGGGTCCAACGATCTCTACTTTCCAACCAAATACTCTCAACCTTTTCCAGTTCAGTGCAAAGCTTGTTTCTGGAAAATGCATTGGTCTTATTGGAGAAATCCTCAGTACAATGCCATACGATTCTTTATGACCATTGTCATTGGTGTTTTATTTGGTCTTATCTTCTGGCAGAAGGGACAACAGACAGCCCAACAGCAAGATCTGATGAACTTTTTGGGAGCTATGTATGCTGCTGTGCTTTTCCTTGGAGCCACGAATGCTTCTGCAGTCCAATCTGTTGTCGCCATTGAAAGAACAGTTTTCTACCGCGAAAGAGCTGCCGGAATGTATTCTGAACTGCCTTATGCATTTGCTCAGGTGGCTATAGAGACAATCTATGTTGCGATCCAAACATTTATATATACACTTCTTCTCTATTCCATGATTGGATTCAAGTGGAAAATCGAAAAATTCCTTTGGTTTTACTACTATATCCTAATGTGTTTCATCTACTTCACAATGTACGGTATGATGGTGGTTGCACTGACTCCGGGCCACCAGATTGCTGCCATTGTCATGTCCTTCTTCCTCAGTTTCTGGAACTTGTTTTCTGGCTTCCTCATTCCAAGACCGCAAATACCAATCTGGTGGAGGTGGTACTACTGGGCTTCCCCTGTGGCCTGGACACTTTATGGCCTTGTGACATCCCAAGTGGGTGACAAGAATGCTGACCTTATCTTGCCTGGATATGGCACCATGCCATTGAAGAAGTTCCTGAAAGATGACTTTGGTTTTGAGTATGACTTCCTTCCGGTCGTCGCCGCCGCACACGTCGGCTGGGTTCTTCTTTTCTTCTTTGTGTTTGCCTATGGCATCAAGTTCCTCAACTTCCAAAGGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1233

Amino Acids

140.2

Weight (kDa)

8.15

Isoelectric Point (pI)

36.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000245)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66950 AT2G36380
fragaria_vesca FvH4_3g29700 FvH4_3g29700 FvH4_3g29700 FvH4_3g29700 FvH4_3g29710 FvH4_3g29740 FvH4_3g29740 FvH4_3g29740 FvH4_3g29740 FvH4_3g29740 FvH4_3g29760 FvH4_3g29781 FvH4_6g18150 FvH4_6g18150 FvH4_6g18150 FvH4_6g18150 FvH4_6g18150
malus_domestica MD02G1254900.v1.1 MD03G1119400.v1.1 MD03G1136800.v1.1 MD03G1137000.v1.1 MD04G1187500.v1.1 MD08G1208900.v1.1 MD08G1209000.v1.1 MD11G1159000.v1.1 MD11G1159100.v1.1 MD11G1159200.v1.1 MD12G1020800.v1.1 MD12G1021000.v1.1 MD14G1017900.v1.1 MD14G1018100.v1.1
prunus_persica Prupe.2G084300_v2.0.a1 Prupe.2G084300_v2.0.a1 Prupe.6G121200_v2.0.a1 Prupe.6G121200_v2.0.a1 Prupe.6G121500_v2.0.a1 Prupe.6G121800_v2.0.a1 Prupe.6G121900_v2.0.a1 Prupe.6G122100_v2.0.a1 Prupe.7G115200_v2.0.a1 Prupe.7G115200_v2.0.a1
pyrus_communis pycom02g21460 pycom03g09270 pycom03g09290 pycom07g05130 pycom08g18050 pycom11g12950 pycom11g12960 pycom11g12970 pycom12g01950 pycom12g01980 pycom14g01620 pycom14g01710 pycom14g01720 pycom16g12190
rosa_chinensis RchiOBHm_Chr1g0340571 RchiOBHm_Chr2g0107751 RchiOBHm_Chr3g0472451 RchiOBHm_Chr5g0055051 RchiOBHm_Chr5g0055091 RchiOBHm_Chr5g0055111 RchiOBHm_Chr5g0055121 RchiOBHm_Chr5g0055131 RchiOBHm_Chr5g0062371 RchiOBHm_Chr6g0265121 RchiOBHm_Chr6g0275771 RchiOBHm_Chr7g0187231
rosa_laevigata RLG00000006319 RLG00000013820 RLG00000024078 RLG00000034960 RLG00000034963 RLG00000034965 RLG00000034966 RLG00000034968 RLG00000034970
rosa_multiflora Rmu_co8358859.1_g000001 Rmu_co8422375.1_g000001 Rmu_co8427767.1_g000001 Rmu_co8474941.1_g000001 Rmu_sc0000925.1_g000009 Rmu_sc0001694.1_g000015 Rmu_sc0001694.1_g000022 Rmu_sc0001694.1_g000024 Rmu_sc0001694.1_g000030 Rmu_sc0003993.1_g000001 Rmu_sc0010632.1_g000001 Rmu_sc0035175.1_g000001
rosa_roxburghii Rroxscaffold_1G00025230 Rroxscaffold_1G00025240 Rroxscaffold_1G00025300 Rroxscaffold_1G00025320 Rroxscaffold_1G00025330 Rroxscaffold_1G00025360 Rroxscaffold_1G00025440 Rroxscaffold_1G00033590 Rroxscaffold_6G00408900
rosa_rugosa Rorug03G0125500.1 Rorug03G0125600.1 Rorug05G0290600 Rorug05G0291100 Rorug05G0291200 Rorug05G0291300
rosa_samantha Rh1CG035400 Rh1CG243200 Rh2DG410600 Rh3AG177500 Rh3CG193800 Rh3CG200900 Rh3DG171700 Rh5AG043000 Rh5AG360500 Rh5AG360900 Rh5AG361000 Rh5AG361300 Rh5AG441100 Rh5CG393700 Rh5CG393900 Rh5CG394200 Rh5DG247300 Rh5DG385200 Rh5DG385500 Rh5DG385800 Rh6BG272200 Rh6DG204000 Rh7AG084700 Rh7AG178300
rosa_wichuraiana Rw0G022120 Rw3G016410 Rw5G033910 Rw5G033920 Rw5G033940 Rw5G033960 Rw5G033970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2343
AatII GACGTC 1 cut(s) 2064
Acc36I ACCTGC 1 cut(s) 2060
Acc65I GGTACC 1 cut(s) 1406
AccB1I GGYRCC 2 cut(s) 1406, 3548
AccB7I CCANNNNNTGG 1 cut(s) 181
AccII CGCG 4 cut(s) 387, 621, 1562, 3126
AciI CCGC 7 cut(s) 493, 546, 2186, 3124, 3428, 3618, 3621
AclWI GGATC 6 cut(s) 1213, 1226, 1259, 1568, 1851, 3196
AcoI YGGCCR 4 cut(s) 40, 373, 805, 2605
AcsI RAATTY 6 cut(s) 15, 1061, 1538, 1827, 2314, 3266
AcuI CTGAAG 3 cut(s) 772, 1066, 1806
AcvI CACGTG 1 cut(s) 649
AcyI GRCGYC 1 cut(s) 2061
AfiI CCNNNNNNNGG 7 cut(s) 181, 1356, 1735, 1859, 2432, 2816, 3545
AflIII ACRYGT 3 cut(s) 74, 646, 3625
AhdI GACNNNNNGTC 1 cut(s) 2433
AhlI ACTAGT 1 cut(s) 278
AjiI CACGTC 1 cut(s) 3628
AjnI CCWGG 7 cut(s) 2064, 2222, 2444, 2647, 2781, 3476, 3538
AjuI GAANNNNNNNTTGG 4 cut(s) 266, 298, 2299, 2331
Alw21I GWGCWC 3 cut(s) 567, 856, 1669
Alw26I GTCTC 6 cut(s) 122, 268, 2362, 2441, 2714, 3179
AlwI GGATC 6 cut(s) 1213, 1226, 1259, 1568, 1851, 3196
AlwNI CAGNNNCTG 2 cut(s) 2788, 3393
Ama87I CYCGRG 4 cut(s) 142, 230, 2332, 2816
ApoI RAATTY 6 cut(s) 15, 1061, 1538, 1827, 2314, 3266
ArsI GACNNNNNNTTYG 2 cut(s) 3572, 3604
AseI ATTAAT 1 cut(s) 1092
Asp700I GAANNNNTTC 3 cut(s) 1538, 3145, 3566
Asp718I GGTACC 1 cut(s) 1406
AspA2I CCTAGG 1 cut(s) 1435
AspLEI GCGC 4 cut(s) 387, 948, 1562, 1564
AspS9I GGNCC 8 cut(s) 413, 614, 715, 1210, 2100, 2597, 2820, 3349
AsuC2I CCSGG 7 cut(s) 397, 2004, 2333, 2334, 2817, 2818, 3348
AsuHPI GGTGA 4 cut(s) 356, 712, 2510, 3524
AsuII TTCGAA 1 cut(s) 642
AsuNHI GCTAGC 2 cut(s) 1292, 1394
AvaI CYCGRG 4 cut(s) 142, 230, 2332, 2816
AvaII GGWCC 7 cut(s) 413, 614, 715, 1210, 2100, 2597, 2820
AvrII CCTAGG 1 cut(s) 1435
AxyI CCTNAGG 1 cut(s) 1214
BaeGI GKGCMC 3 cut(s) 665, 1043, 2658
BalI TGGCCA 3 cut(s) 42, 375, 807
BamHI GGATCC 1 cut(s) 1218
BanI GGYRCC 2 cut(s) 1406, 3548
BanII GRGCYC 2 cut(s) 567, 856
BauI CACGAG 1 cut(s) 2145
BbrPI CACGTG 1 cut(s) 649
BbsI GAAGAC 2 cut(s) 281, 1946
Bbv12I GWGCWC 3 cut(s) 567, 856, 1669
BccI CCATC 7 cut(s) 427, 1294, 1333, 1548, 2336, 2651, 3322
BceAI ACGGC 1 cut(s) 2592
BcgI CGANNNNNNTGC 8 cut(s) 221, 255, 903, 937, 937, 971, 3116, 3150
BciT130I CCWGG 7 cut(s) 2066, 2224, 2446, 2649, 2783, 3478, 3540
BclI TGATCA 1 cut(s) 2790
BcnI CCSGG 7 cut(s) 397, 2004, 2333, 2334, 2817, 2818, 3348
BcoDI GTCTC 6 cut(s) 122, 268, 2362, 2441, 2714, 3179
BcuI ACTAGT 1 cut(s) 278
BfaI CTAG 7 cut(s) 168, 279, 1293, 1395, 1436, 1620, 2339
BfmI CTRYAG 6 cut(s) 539, 751, 1593, 2712, 3081, 3180
BfuAI ACCTGC 1 cut(s) 2060
BglII AGATCT 1 cut(s) 3016
BlnI CCTAGG 1 cut(s) 1435
Bme18I GGWCC 7 cut(s) 413, 614, 715, 1210, 2100, 2597, 2820
BmeRI GACNNNNNGTC 1 cut(s) 2433
BmeT110I CYCGRG 4 cut(s) 142, 230, 2332, 2816
BmgBI CACGTC 1 cut(s) 3628
BmgT120I GGNCC 8 cut(s) 413, 614, 715, 1210, 2100, 2597, 2820, 3349
BmrI ACTGGG 1 cut(s) 3469
BmsI GCATC 5 cut(s) 229, 1467, 2431, 2682, 3678
BmtI GCTAGC 2 cut(s) 1296, 1398
BmuI ACTGGG 1 cut(s) 3469
BoxI GACNNNNGTC 1 cut(s) 2951
BpiI GAAGAC 2 cut(s) 281, 1946
BplI GAGNNNNNCTC 2 cut(s) 2902, 2934
Bpu10I CCTNAGC 1 cut(s) 3171
Bpu14I TTCGAA 1 cut(s) 642
BpuEI CTTGAG 5 cut(s) 178, 287, 653, 1598, 2717
BpuMI CCSGG 7 cut(s) 397, 2004, 2333, 2334, 2817, 2818, 3348
Bsa29I ATCGAT 1 cut(s) 6
BsaAI YACGTR 1 cut(s) 649
BsaBI GATNNNNATC 2 cut(s) 986, 1250
BsaHI GRCGYC 1 cut(s) 2061
BsaI GGTCTC 1 cut(s) 2441
BsaWI WCCGGW 3 cut(s) 368, 550, 3607
BsaXI ACNNNNNCTCC 6 cut(s) 2568, 2598, 2744, 2774, 2797, 2827
Bsc4I CCNNNNNNNGG 7 cut(s) 181, 1356, 1735, 1859, 2432, 2816, 3545
Bse118I RCCGGY 2 cut(s) 415, 2809
Bse1I ACTGG 6 cut(s) 1034, 1125, 1649, 2086, 2864, 3464
Bse21I CCTNAGG 1 cut(s) 1214
Bse3DI GCAATG 3 cut(s) 1131, 1362, 1704
Bse8I GATNNNNATC 2 cut(s) 986, 1250
BseBI CCWGG 7 cut(s) 2066, 2224, 2446, 2649, 2783, 3478, 3540
BseCI ATCGAT 1 cut(s) 6
BseGI GGATG 9 cut(s) 442, 520, 856, 1482, 2425, 2643, 2674, 2697, 3500
BseJI GATNNNNATC 2 cut(s) 986, 1250
BseLI CCNNNNNNNGG 7 cut(s) 181, 1356, 1735, 1859, 2432, 2816, 3545
BseMI GCAATG 3 cut(s) 1131, 1362, 1704
BseMII CTCAG 8 cut(s) 273, 279, 762, 841, 1228, 2933, 3185, 3400
BseNI ACTGG 6 cut(s) 1034, 1125, 1649, 2086, 2864, 3464
BsePI GCGCGC 1 cut(s) 1560
BseRI GAGGAG 1 cut(s) 2752
BseSI GKGCMC 3 cut(s) 665, 1043, 2658
BseX3I CGGCCG 1 cut(s) 2605
BseYI CCCAGC 1 cut(s) 3633
BsgI GTGCAG 2 cut(s) 1353, 2494
Bsh1236I CGCG 4 cut(s) 387, 621, 1562, 3126
Bsh1285I CGRYCG 2 cut(s) 2608, 3612
BshNI GGYRCC 2 cut(s) 1406, 3548
BshVI ATCGAT 1 cut(s) 6
BsiEI CGRYCG 2 cut(s) 2608, 3612
BsiHKAI GWGCWC 3 cut(s) 567, 856, 1669
BsiHKCI CYCGRG 4 cut(s) 142, 230, 2332, 2816
BslFI GGGAC 5 cut(s) 159, 1333, 2113, 2349, 3006
BslI CCNNNNNNNGG 7 cut(s) 181, 1356, 1735, 1859, 2432, 2816, 3545
BsmAI GTCTC 6 cut(s) 122, 268, 2362, 2441, 2714, 3179
BsmFI GGGAC 5 cut(s) 159, 1333, 2113, 2349, 3006
BsmI GAATGC 5 cut(s) 242, 1319, 1525, 3079, 3526
Bso31I GGTCTC 1 cut(s) 2441
BsoBI CYCGRG 4 cut(s) 142, 230, 2332, 2816
Bsp119I TTCGAA 1 cut(s) 642
Bsp1286I GDGCHC 6 cut(s) 567, 665, 856, 1043, 1669, 2658
Bsp1407I TGTACA 1 cut(s) 960
Bsp19I CCATGG 1 cut(s) 522
BspACI CCGC 7 cut(s) 493, 546, 2186, 3124, 3428, 3618, 3621
BspCNI CTCAG 8 cut(s) 272, 278, 763, 840, 1227, 2932, 3184, 3399
BspDI ATCGAT 1 cut(s) 6
BspFNI CGCG 4 cut(s) 387, 621, 1562, 3126
BspHI TCATGA 2 cut(s) 246, 1530
BspMAI CTGCAG 3 cut(s) 543, 2716, 3085
BspMI ACCTGC 1 cut(s) 2060
BspOI GCTAGC 2 cut(s) 1296, 1398
BspPI GGATC 6 cut(s) 1213, 1226, 1259, 1568, 1851, 3196
BspQI GCTCTTC 1 cut(s) 572
BspT104I TTCGAA 1 cut(s) 642
BspT107I GGYRCC 2 cut(s) 1406, 3548
BspTNI GGTCTC 1 cut(s) 2441
BsrDI GCAATG 3 cut(s) 1131, 1362, 1704
BsrFI RCCGGY 2 cut(s) 415, 2809
BsrGI TGTACA 1 cut(s) 960
BsrI ACTGG 6 cut(s) 1034, 1125, 1649, 2086, 2864, 3464
BssAI RCCGGY 2 cut(s) 415, 2809
BssHII GCGCGC 1 cut(s) 1560
BssNI GRCGYC 1 cut(s) 2061
BssSI CACGAG 1 cut(s) 2145
BssT1I CCWWGG 5 cut(s) 522, 610, 1435, 1472, 3061
Bst2BI CACGAG 1 cut(s) 2145
Bst2UI CCWGG 7 cut(s) 2066, 2224, 2446, 2649, 2783, 3478, 3540
Bst6I CTCTTC 6 cut(s) 193, 540, 572, 1689, 1694, 2360
BstACI GRCGYC 1 cut(s) 2061
BstAUI TGTACA 1 cut(s) 960
BstBAI YACGTR 1 cut(s) 649
BstBI TTCGAA 1 cut(s) 642
BstC8I GCNNGC 8 cut(s) 183, 546, 809, 1294, 1396, 1400, 1562, 1893
BstDSI CCRYGG 1 cut(s) 522
BstEII GGTNACC 2 cut(s) 1197, 2117
BstF5I GGATG 9 cut(s) 442, 520, 856, 1482, 2425, 2643, 2674, 2697, 3500
BstFNI CGCG 4 cut(s) 387, 621, 1562, 3126
BstHHI GCGC 4 cut(s) 387, 948, 1562, 1564
BstMAI GTCTC 6 cut(s) 122, 268, 2362, 2441, 2714, 3179
BstMCI CGRYCG 2 cut(s) 2608, 3612
BstNI CCWGG 7 cut(s) 2066, 2224, 2446, 2649, 2783, 3478, 3540
BstNSI RCATGY 4 cut(s) 78, 493, 918, 2192
BstPAI GACNNNNGTC 1 cut(s) 2951
BstPI GGTNACC 2 cut(s) 1197, 2117
BstSFI CTRYAG 6 cut(s) 539, 751, 1593, 2712, 3081, 3180
BstSLI GKGCMC 3 cut(s) 665, 1043, 2658
BstUI CGCG 4 cut(s) 387, 621, 1562, 3126
BstV2I GAAGAC 2 cut(s) 281, 1946
BstX2I RGATCY 3 cut(s) 1218, 1573, 3016
BstXI CCANNNNNNTGG 2 cut(s) 2383, 3444
BstYI RGATCY 3 cut(s) 1218, 1573, 3016
BstZI CGGCCG 1 cut(s) 2605
Bsu15I ATCGAT 1 cut(s) 6
Bsu36I CCTNAGG 1 cut(s) 1214
BsuTUI ATCGAT 1 cut(s) 6
BtgI CCRYGG 1 cut(s) 522
BtrI CACGTC 1 cut(s) 3628
BtsCI GGATG 9 cut(s) 442, 520, 856, 1482, 2425, 2643, 2674, 2697, 3500
BtsI GCAGTG 1 cut(s) 2703
BtsIMutI CAGTG 9 cut(s) 126, 1041, 1815, 1993, 2158, 2490, 2703, 2876, 3338
BveI ACCTGC 1 cut(s) 2060
Cac8I GCNNGC 8 cut(s) 183, 546, 809, 1294, 1396, 1400, 1562, 1893
CaiI CAGNNNCTG 2 cut(s) 2788, 3393
CciI TCATGA 2 cut(s) 246, 1530
CfoI GCGC 4 cut(s) 387, 948, 1562, 1564
Cfr10I RCCGGY 2 cut(s) 415, 2809
Cfr13I GGNCC 8 cut(s) 413, 614, 715, 1210, 2100, 2597, 2820, 3349
Cfr9I CCCGGG 2 cut(s) 2332, 2816
ClaI ATCGAT 1 cut(s) 6
DrdI GACNNNNNNGTC 1 cut(s) 2343
DriI GACNNNNNGTC 1 cut(s) 2433
DseDI GACNNNNNNGTC 1 cut(s) 2343
EaeI YGGCCR 4 cut(s) 40, 373, 805, 2605
EagI CGGCCG 1 cut(s) 2605
Eam1104I CTCTTC 6 cut(s) 193, 540, 572, 1689, 1694, 2360
Eam1105I GACNNNNNGTC 1 cut(s) 2433
EarI CTCTTC 6 cut(s) 193, 540, 572, 1689, 1694, 2360
Ecl136II GAGCTC 2 cut(s) 565, 854
EclXI CGGCCG 1 cut(s) 2605
Eco130I CCWWGG 5 cut(s) 522, 610, 1435, 1472, 3061
Eco147I AGGCCT 1 cut(s) 785
Eco24I GRGCYC 2 cut(s) 567, 856
Eco31I GGTCTC 1 cut(s) 2441
Eco32I GATATC 1 cut(s) 334
Eco47I GGWCC 7 cut(s) 413, 614, 715, 1210, 2100, 2597, 2820
Eco52I CGGCCG 1 cut(s) 2605
Eco53kI GAGCTC 2 cut(s) 565, 854
Eco57I CTGAAG 3 cut(s) 772, 1066, 1806
Eco72I CACGTG 1 cut(s) 649
Eco81I CCTNAGG 1 cut(s) 1214
Eco88I CYCGRG 4 cut(s) 142, 230, 2332, 2816
Eco91I GGTNACC 2 cut(s) 1197, 2117
EcoICRI GAGCTC 2 cut(s) 565, 854
EcoO109I RGGNCCY 1 cut(s) 1210
EcoO65I GGTNACC 2 cut(s) 1197, 2117
EcoRI GAATTC 1 cut(s) 1538
EcoRII CCWGG 7 cut(s) 2064, 2222, 2444, 2647, 2781, 3476, 3538
EcoRV GATATC 1 cut(s) 334
EcoT14I CCWWGG 5 cut(s) 522, 610, 1435, 1472, 3061
EcoT22I ATGCAT 3 cut(s) 244, 2898, 3166
EcoT38I GRGCYC 2 cut(s) 567, 856
ErhI CCWWGG 5 cut(s) 522, 610, 1435, 1472, 3061
FalI AAGNNNNNCTT 2 cut(s) 1950, 1982
FaqI GGGAC 5 cut(s) 159, 1333, 2113, 2349, 3006
FauI CCCGC 2 cut(s) 553, 2193
FbaI TGATCA 1 cut(s) 2790
FokI GGATG 9 cut(s) 449, 527, 843, 1489, 2432, 2630, 2681, 2704, 3487
FriOI GRGCYC 2 cut(s) 567, 856
FspBI CTAG 7 cut(s) 168, 279, 1293, 1395, 1436, 1620, 2339
GlaI GCGC 4 cut(s) 386, 947, 1561, 1563
GsaI CCCAGC 1 cut(s) 3637
HhaI GCGC 4 cut(s) 387, 948, 1562, 1564
Hin1I GRCGYC 1 cut(s) 2061
Hin6I GCGC 4 cut(s) 385, 946, 1560, 1562
HinP1I GCGC 4 cut(s) 385, 946, 1560, 1562
HindIII AAGCTT 2 cut(s) 2621, 2877
HphI GGTGA 4 cut(s) 356, 712, 2510, 3524
Hpy99I CGWCG 4 cut(s) 575, 2555, 3617, 3632
HpyCH4IV ACGT 4 cut(s) 648, 2061, 2572, 3627
HpySE526I ACGT 4 cut(s) 648, 2061, 2572, 3627
Hsp92I GRCGYC 1 cut(s) 2061
HspAI GCGC 4 cut(s) 385, 946, 1560, 1562
KpnI GGTACC 1 cut(s) 1410
Ksp22I TGATCA 1 cut(s) 2790
LguI GCTCTTC 1 cut(s) 572
LweI GCATC 5 cut(s) 229, 1467, 2431, 2682, 3678
MaeI CTAG 7 cut(s) 168, 279, 1293, 1395, 1436, 1620, 2339
MaeII ACGT 4 cut(s) 648, 2061, 2572, 3627
MfeI CAATTG 2 cut(s) 497, 1970
MflI RGATCY 3 cut(s) 1218, 1573, 3016
MhlI GDGCHC 6 cut(s) 567, 665, 856, 1043, 1669, 2658
MlsI TGGCCA 3 cut(s) 42, 375, 807
MluNI TGGCCA 3 cut(s) 42, 375, 807
MlyI GAGTC 4 cut(s) 563, 1936, 2716, 3337
MmeI TCCRAC 3 cut(s) 1903, 2847, 2864
Mox20I TGGCCA 3 cut(s) 42, 375, 807
Mph1103I ATGCAT 3 cut(s) 244, 2898, 3166
MroXI GAANNNNTTC 3 cut(s) 1538, 3145, 3566
MscI TGGCCA 3 cut(s) 42, 375, 807
MslI CAYNNNNRTG 3 cut(s) 2381, 2414, 3371
Msp20I TGGCCA 3 cut(s) 42, 375, 807
MspA1I CMGCKG 1 cut(s) 821
MunI CAATTG 2 cut(s) 497, 1970
Mva1269I GAATGC 5 cut(s) 242, 1319, 1525, 3079, 3526
MvaI CCWGG 7 cut(s) 2066, 2224, 2446, 2649, 2783, 3478, 3540
MvnI CGCG 4 cut(s) 387, 621, 1562, 3126
NciI CCSGG 7 cut(s) 397, 2004, 2333, 2334, 2817, 2818, 3348
NcoI CCATGG 1 cut(s) 522
NheI GCTAGC 2 cut(s) 1292, 1394
NmeAIII GCCGAG 1 cut(s) 2583
NmuCI GTSAC 8 cut(s) 286, 388, 700, 871, 2609, 2701, 3496, 3512
NsiI ATGCAT 3 cut(s) 244, 2898, 3166
NspI RCATGY 4 cut(s) 78, 493, 918, 2192
NspV TTCGAA 1 cut(s) 642
PagI TCATGA 2 cut(s) 246, 1530
PauI GCGCGC 1 cut(s) 1560
PceI AGGCCT 1 cut(s) 785
PciI ACATGT 1 cut(s) 74
PciSI GCTCTTC 1 cut(s) 572
PctI GAATGC 5 cut(s) 242, 1319, 1525, 3079, 3526
PdmI GAANNNNTTC 3 cut(s) 1538, 3145, 3566
PfeI GAWTC 9 cut(s) 216, 654, 1238, 1626, 1739, 2204, 2543, 2937, 3246
PflMI CCANNNNNTGG 1 cut(s) 181
PleI GAGTC 4 cut(s) 562, 1936, 2716, 3337
PmaCI CACGTG 1 cut(s) 649
PmlI CACGTG 1 cut(s) 649
PpsI GAGTC 4 cut(s) 562, 1936, 2716, 3337
Ppu21I YACGTR 1 cut(s) 649
PpuMI RGGWCCY 1 cut(s) 1210
PscI ACATGT 1 cut(s) 74
PshAI GACNNNNGTC 1 cut(s) 2951
PshBI ATTAAT 1 cut(s) 1092
Psp124BI GAGCTC 2 cut(s) 567, 856
Psp5II RGGWCCY 1 cut(s) 1210
Psp6I CCWGG 7 cut(s) 2064, 2222, 2444, 2647, 2781, 3476, 3538
PspCI CACGTG 1 cut(s) 649
PspEI GGTNACC 2 cut(s) 1197, 2117
PspFI CCCAGC 1 cut(s) 3633
PspGI CCWGG 7 cut(s) 2064, 2222, 2444, 2647, 2781, 3476, 3538
PspPI GGNCC 8 cut(s) 413, 614, 715, 1210, 2100, 2597, 2820, 3349
PspPPI RGGWCCY 1 cut(s) 1210
PstI CTGCAG 3 cut(s) 543, 2716, 3085
PstNI CAGNNNCTG 2 cut(s) 2788, 3393
PsuI RGATCY 3 cut(s) 1218, 1573, 3016
PteI GCGCGC 1 cut(s) 1560
PvuII CAGCTG 1 cut(s) 821
RseI CAYNNNNRTG 3 cut(s) 2381, 2414, 3371
SacI GAGCTC 2 cut(s) 567, 856
SapI GCTCTTC 1 cut(s) 572
Sau96I GGNCC 8 cut(s) 413, 614, 715, 1210, 2100, 2597, 2820, 3349
SchI GAGTC 4 cut(s) 563, 1936, 2716, 3337
SduI GDGCHC 6 cut(s) 567, 665, 856, 1043, 1669, 2658
SfaNI GCATC 5 cut(s) 229, 1467, 2431, 2682, 3678
SfcI CTRYAG 6 cut(s) 539, 751, 1593, 2712, 3081, 3180
SfuI TTCGAA 1 cut(s) 642
SinI GGWCC 7 cut(s) 413, 614, 715, 1210, 2100, 2597, 2820
SmaI CCCGGG 2 cut(s) 2334, 2818
SmiMI CAYNNNNRTG 3 cut(s) 2381, 2414, 3371
SmlI CTYRAG 5 cut(s) 193, 302, 632, 1613, 2696
SmoI CTYRAG 5 cut(s) 193, 302, 632, 1613, 2696
SpeI ACTAGT 1 cut(s) 278
SseBI AGGCCT 1 cut(s) 785
SsiI CCGC 7 cut(s) 493, 546, 2186, 3124, 3428, 3618, 3621
SspI AATATT 3 cut(s) 728, 2010, 2633
SspMI CTAG 7 cut(s) 168, 279, 1293, 1395, 1436, 1620, 2339
SstI GAGCTC 2 cut(s) 567, 856
StuI AGGCCT 1 cut(s) 785
StyI CCWWGG 5 cut(s) 522, 610, 1435, 1472, 3061
TaiI ACGT 4 cut(s) 651, 2064, 2575, 3630
TaqI TCGA 8 cut(s) 6, 570, 642, 923, 2094, 2439, 2550, 3261
TatI WGTACW 4 cut(s) 960, 1045, 2804, 2922
TauI GCSGC 3 cut(s) 496, 3620, 3623
TfiI GAWTC 9 cut(s) 216, 654, 1238, 1626, 1739, 2204, 2543, 2937, 3246
TscAI CASTG 9 cut(s) 126, 1041, 1815, 1993, 2158, 2490, 2710, 2876, 3345
TseFI GTSAC 8 cut(s) 286, 388, 700, 871, 2609, 2701, 3496, 3512
Tsp45I GTSAC 8 cut(s) 286, 388, 700, 871, 2609, 2701, 3496, 3512
TspGWI ACGGA 3 cut(s) 1527, 2219, 2377
TspMI CCCGGG 2 cut(s) 2332, 2816
TspRI CASTG 9 cut(s) 126, 1041, 1815, 1993, 2158, 2490, 2710, 2876, 3345
Van91I CCANNNNNTGG 1 cut(s) 181
VpaK11BI GGWCC 7 cut(s) 413, 614, 715, 1210, 2100, 2597, 2820
VspI ATTAAT 1 cut(s) 1092
XapI RAATTY 6 cut(s) 15, 1061, 1538, 1827, 2314, 3266
XbaI TCTAGA 1 cut(s) 1619
XceI RCATGY 4 cut(s) 78, 493, 918, 2192
XmaI CCCGGG 2 cut(s) 2332, 2816
XmaJI CCTAGG 1 cut(s) 1435
XmnI GAANNNNTTC 3 cut(s) 1538, 3145, 3566
XspI CTAG 7 cut(s) 168, 279, 1293, 1395, 1436, 1620, 2339
ZraI GACGTC 1 cut(s) 2062
Zsp2I ATGCAT 3 cut(s) 244, 2898, 3166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.