Rmu_sc0003993.1_g000001
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003993.1
OG0000245
Physical Location & Seq
Reverse (-)
2 .. 5467
5466 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003993.1_g000001.1.cds

Sequence Viewer

Length: 3564 bp
atggcgttggctttggccggagacgatctggcccggtcgacgagcagccggagaagctatggttcacatgggagcaagcggagctgggcgtccacaagtttccgggaggtgtggcaggtcccgccggacgtgttcaaccggagtggccggcaggacgaggatgaagagctccggtgggccgctatcgagcggcttccgacttacgacagacttagaagagggatgctccggcaggtactcgacaatggacgagtggttactgatgaagtggatgttacgaggcttggagtgcaagacaagaaggtgttgatggagaacatactcaaagttgtggaagatgataatgaaaagtttctaaggagacttagagacagaactgacaggtttgagactgtgaagctcactcttaacaaagaaaaaaaaaactatgaaaagtttctaaggagacttagagacagaactgacagagttgggattgagattccgaagattgaagttagatatgagcatctgtcgattgacggagatgtgtatgttgggaaaagagcactgcctactctgcttaatgccaccttgaacactatagagagtgttctgggtttgattcgtcttgctccatcaaagaagagaaagatccagatacttaaagatgttaatggaatagtcaaaccttctaggatgaccctacttttgggtcctccaggtgcaggtaaaacaacattgctgttggcacttgctgggaagcttgatgacgatctaagggtatctgggaacatcacttactgtggtcatgaattgagagagtttgttccccgaaggacatgtgcttatatcagtcaacatgatcttcactatggagaaatgacagtgagggagacattggatttttcgggacgatgcttaggtgtggggactagatatgaaatgctggctgaactttccagaagagaaaaagaagcaggaatcaagccggatcctgagattgatgcattcatgaaagccacctcagtttcaggcctgaagactagtttggtcacagattatgttctcaagattcttggtttggatatctgttctgatattatggttggtgatgaaatgagaaggggtatttctggtggccagaaaaagcgtgtcacgactggggaaatgttggttggaccagcaaaggtgcttttaatggatgaaatttcaacagggttagacagttccacaacttttcagatatgcaggtacatgaggcaaatggtacatataatggatgtcacaatggtcatctctcttcttcaaccagcaccagagacatttgatctgtttgatgaccttatcttactttcagaaggtcagattgtgtaccaaggtccgcgtgagagtgttctcgagttctttgaatacatgggtttcaaatgcccggagaggaaaggagttgctgactttttgcaagaagtaacatctaagaaggatcaagaacaatactggttcaagaaaagcgaaccttacagatacattgaagtttctgagtttgttgagtgcttcaacaatttccaatatggccaacagattgcatcggaacttggggttccttatgaaaagtcaagagctcacccagctgcattagtcaaagagaaatatggtatttcaaattgggaactgttcaaggcttgttttgcaagggagtggctgctaatgaagcgcaattcttttgtgtatgtattcaagacaacccagataacaattatgtcagtaattgcgttcacagtgttccttaggactcaaatgccagtgggaactgtacaagatggaggaaaatttttcggagcgctgttttttagtctgattaatgtcatgttcaatggaatggcagaactggcaatgactgttttcaggcttcctgtgttttataaacagagggatttcttgttttatcctgcttgggcttttggtttgcccatttgggtccttaggatccccctatctttaatggagtcggcaatatggattgttcttacttattacaccattggatttgctccagctgctagcagatttttcagacagtttttggctttttttggcattcatcaaatggcactgtccctctttcggttcattgctgctcttggaagaacacaaacggttgcaaatactctcggtaccttcaccttgcttatggtgtttgttctgggaggatttattgttgccaaaaatgacattgagccttggatgatatggggctactatatatctcctatgatgtatggacagaatgcaattgtcatgaacgaattcctcgataaaagatggagcgcacgaaatacagatccaagaattaatgaacctacagttgggaaagtcctccttaagtccagaggattcttcacagatgaatattggttctggatatgtattggagcactatttggattttctcttctctttaacatcttattcattgctgcattgacttttctgaatcctcttggagattcgaaggctgtcatagctgatgaaaaaagtgagaaaaagaaaaggaagtcatcaactgaaggtattgatatggcatttaggagttcgtcagagcatgcaccaacgaaaggaatggttttgcccttccaacccctttcactcgcatttaaccatgtgaactactatgtcgatatgccagctgaaatgaagagtcaaggagttgatcaagatcgccttcagctcctacgagatgtcagtggtgcttttagaccaggagtattgacagcacttgtaggtgttagtggtgctggaaaaacaactcttatggacgtcttggcaggaagaaagacaggtggttatattgaaggaagtattaacatctccggttatccaaagaaacaagaaacatttgctcgtgttagtggttattgtgagcagaatgacatccattcaccacatgttactgtatacgaatctcttctgtattcagcctggctccgtctttcttcaagtgtaaagacccgaacaagaaagatgtttgttgaggaagtcatggagttggttgagcttaatcccattagagatgctctagttggccttccgggaatagatggtctttcaacagaacaaaggaagagactaaccatagccgtggagctggtagccaacccctctattattttcatggatgaaccaacatctggacttgatgccagggctgctgccatcgttatgcgtactgtgagaaacacagtggacactggaagaactgttgtctgcacgattcatcagccaagcatagatatttttgaagcttttgatgagctgcttctgatgaaacgaggagggcaagttatatatgctggacctctaggtcgccaatctcacaagcttgtagaatattttgaagctgttccaggggtcactaagattaaggatggttacaatcctgctacatggatgcttgaggttactgctccttcagttgaggctcaattggatgtggactttgcagacatttatgccaattcctcgctttaccagaggaatcaagagcttatcaaagagcttagtactccggtgccagggtccaaggat
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1188

Amino Acids

134.47

Weight (kDa)

8.59

Isoelectric Point (pI)

40.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000245)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66950 AT2G36380
fragaria_vesca FvH4_3g29700 FvH4_3g29700 FvH4_3g29700 FvH4_3g29700 FvH4_3g29710 FvH4_3g29740 FvH4_3g29740 FvH4_3g29740 FvH4_3g29740 FvH4_3g29740 FvH4_3g29760 FvH4_3g29781 FvH4_6g18150 FvH4_6g18150 FvH4_6g18150 FvH4_6g18150 FvH4_6g18150
malus_domestica MD02G1254900.v1.1 MD03G1119400.v1.1 MD03G1136800.v1.1 MD03G1137000.v1.1 MD04G1187500.v1.1 MD08G1208900.v1.1 MD08G1209000.v1.1 MD11G1159000.v1.1 MD11G1159100.v1.1 MD11G1159200.v1.1 MD12G1020800.v1.1 MD12G1021000.v1.1 MD14G1017900.v1.1 MD14G1018100.v1.1
prunus_persica Prupe.2G084300_v2.0.a1 Prupe.2G084300_v2.0.a1 Prupe.6G121200_v2.0.a1 Prupe.6G121200_v2.0.a1 Prupe.6G121500_v2.0.a1 Prupe.6G121800_v2.0.a1 Prupe.6G121900_v2.0.a1 Prupe.6G122100_v2.0.a1 Prupe.7G115200_v2.0.a1 Prupe.7G115200_v2.0.a1
pyrus_communis pycom02g21460 pycom03g09270 pycom03g09290 pycom07g05130 pycom08g18050 pycom11g12950 pycom11g12960 pycom11g12970 pycom12g01950 pycom12g01980 pycom14g01620 pycom14g01710 pycom14g01720 pycom16g12190
rosa_chinensis RchiOBHm_Chr1g0340571 RchiOBHm_Chr2g0107751 RchiOBHm_Chr3g0472451 RchiOBHm_Chr5g0055051 RchiOBHm_Chr5g0055091 RchiOBHm_Chr5g0055111 RchiOBHm_Chr5g0055121 RchiOBHm_Chr5g0055131 RchiOBHm_Chr5g0062371 RchiOBHm_Chr6g0265121 RchiOBHm_Chr6g0275771 RchiOBHm_Chr7g0187231
rosa_laevigata RLG00000006319 RLG00000013820 RLG00000024078 RLG00000034960 RLG00000034963 RLG00000034965 RLG00000034966 RLG00000034968 RLG00000034970
rosa_multiflora Rmu_co8358859.1_g000001 Rmu_co8422375.1_g000001 Rmu_co8427767.1_g000001 Rmu_co8474941.1_g000001 Rmu_sc0000925.1_g000009 Rmu_sc0001694.1_g000015 Rmu_sc0001694.1_g000022 Rmu_sc0001694.1_g000024 Rmu_sc0001694.1_g000030 Rmu_sc0003993.1_g000001 Rmu_sc0010632.1_g000001 Rmu_sc0035175.1_g000001
rosa_roxburghii Rroxscaffold_1G00025230 Rroxscaffold_1G00025240 Rroxscaffold_1G00025300 Rroxscaffold_1G00025320 Rroxscaffold_1G00025330 Rroxscaffold_1G00025360 Rroxscaffold_1G00025440 Rroxscaffold_1G00033590 Rroxscaffold_6G00408900
rosa_rugosa Rorug03G0125500.1 Rorug03G0125600.1 Rorug05G0290600 Rorug05G0291100 Rorug05G0291200 Rorug05G0291300
rosa_samantha Rh1CG035400 Rh1CG243200 Rh2DG410600 Rh3AG177500 Rh3CG193800 Rh3CG200900 Rh3DG171700 Rh5AG043000 Rh5AG360500 Rh5AG360900 Rh5AG361000 Rh5AG361300 Rh5AG441100 Rh5CG393700 Rh5CG393900 Rh5CG394200 Rh5DG247300 Rh5DG385200 Rh5DG385500 Rh5DG385800 Rh6BG272200 Rh6DG204000 Rh7AG084700 Rh7AG178300
rosa_wichuraiana Rw0G022120 Rw3G016410 Rw5G033910 Rw5G033920 Rw5G033940 Rw5G033960 Rw5G033970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1902
AasI GACNNNNNNGTC 1 cut(s) 3339
AatII GACGTC 1 cut(s) 2799
Acc36I ACCTGC 4 cut(s) 106, 223, 698, 1221
Acc65I GGTACC 1 cut(s) 2153
AccB1I GGYRCC 2 cut(s) 2153, 3547
AccB7I CCANNNNNTGG 1 cut(s) 3167
AccBSI CCGCTC 1 cut(s) 190
AccI GTMKAC 2 cut(s) 38, 2934
AccII CGCG 1 cut(s) 1366
AciI CCGC 5 cut(s) 79, 122, 180, 190, 1364
AclWI GGATC 7 cut(s) 628, 968, 981, 1470, 1960, 1973, 2315
AcoI YGGCCR 4 cut(s) 15, 145, 1120, 1552
AcsI RAATTY 3 cut(s) 1188, 1808, 2285
AcuI CTGAAG 4 cut(s) 1040, 2565, 2687, 3432
AcyI GRCGYC 2 cut(s) 89, 2796
AfaI GTAC 8 cut(s) 237, 1235, 1251, 1355, 1794, 2155, 3205, 3541
AfeI AGCGCT 1 cut(s) 1821
AfiI CCNNNNNNNGG 7 cut(s) 691, 707, 2103, 2345, 2594, 3167, 3551
AflII CTTAAG 1 cut(s) 2360
AflIII ACRYGT 3 cut(s) 129, 821, 2923
AhlI ACTAGT 1 cut(s) 1025
AjiI CACGTC 1 cut(s) 130
AjnI CCWGG 6 cut(s) 700, 2737, 2957, 3179, 3382, 3550
AjuI GAANNNNNNNTTGG 6 cut(s) 1013, 1045, 1140, 1172, 2328, 2360
Alw21I GWGCWC 4 cut(s) 171, 550, 1603, 2416
Alw26I GTCTC 9 cut(s) 15, 355, 363, 383, 439, 447, 869, 1295, 3097
AlwI GGATC 7 cut(s) 628, 968, 981, 1470, 1960, 1973, 2315
Ama87I CYCGRG 1 cut(s) 1379
Aor51HI AGCGCT 1 cut(s) 1821
AoxI GGCC 8 cut(s) 15, 30, 145, 177, 1015, 1120, 1552, 3061
ApeKI GCWGC 9 cut(s) 45, 1610, 1681, 2036, 2114, 2456, 3185, 3188, 3292
ApoI RAATTY 3 cut(s) 1188, 1808, 2285
ArsI GACNNNNNNTTYG 2 cut(s) 673, 705
AseI ATTAAT 2 cut(s) 1839, 2331
Asp700I GAANNNNTTC 5 cut(s) 351, 435, 2285, 2943, 3378
Asp718I GGTACC 1 cut(s) 2153
AspLEI GCGC 3 cut(s) 1695, 1822, 2309
AspS9I GGNCC 9 cut(s) 31, 118, 177, 695, 1160, 1361, 1957, 3332, 3555
AsuC2I CCSGG 4 cut(s) 34, 104, 1412, 3069
AsuHPI GGTGA 4 cut(s) 1103, 1595, 2152, 2910
AsuII TTCGAA 1 cut(s) 2489
AsuNHI GCTAGC 1 cut(s) 2039
AvaI CYCGRG 1 cut(s) 1379
AvaII GGWCC 7 cut(s) 118, 695, 1160, 1361, 1957, 3332, 3555
AxyI CCTNAGG 2 cut(s) 1766, 1961
BalI TGGCCA 2 cut(s) 1122, 1554
BamHI GGATCC 2 cut(s) 973, 1965
BanI GGYRCC 2 cut(s) 2153, 3547
BanII GRGCYC 2 cut(s) 171, 1603
BauI CACGAG 1 cut(s) 2880
BbsI GAAGAC 1 cut(s) 1028
Bbv12I GWGCWC 4 cut(s) 171, 550, 1603, 2416
BbvI GCAGC 9 cut(s) 57, 1597, 1668, 2023, 2101, 2443, 3172, 3175, 3279
BccI CCATC 7 cut(s) 304, 625, 1793, 2295, 3071, 3200, 3398
BceAI ACGGC 1 cut(s) 3101
BciT130I CCWGG 6 cut(s) 702, 2739, 2959, 3181, 3384, 3552
BclI TGATCA 1 cut(s) 2689
BcnI CCSGG 4 cut(s) 34, 104, 1412, 3069
BcoDI GTCTC 9 cut(s) 15, 355, 363, 383, 439, 447, 869, 1295, 3097
BcuI ACTAGT 1 cut(s) 1025
BfaI CTAG 6 cut(s) 675, 915, 1026, 2040, 3056, 3338
BfmI CTRYAG 2 cut(s) 582, 2340
BfoI RGCGCY 1 cut(s) 1823
BfrI CTTAAG 1 cut(s) 2360
BfuAI ACCTGC 4 cut(s) 106, 223, 698, 1221
BmcAI AGTACT 1 cut(s) 3541
Bme18I GGWCC 7 cut(s) 118, 695, 1160, 1361, 1957, 3332, 3555
BmeT110I CYCGRG 1 cut(s) 1379
BmgBI CACGTC 1 cut(s) 130
BmgT120I GGNCC 9 cut(s) 31, 118, 177, 695, 1160, 1361, 1957, 3332, 3555
BmrI ACTGGG 1 cut(s) 1152
BmsI GCATC 8 cut(s) 213, 517, 887, 976, 1574, 3040, 3166, 3417
BmtI GCTAGC 1 cut(s) 2043
BmuI ACTGGG 1 cut(s) 1152
BpiI GAAGAC 1 cut(s) 1028
BplI GAGNNNNNCTC 2 cut(s) 210, 242
BpmI CTGGAG 2 cut(s) 684, 2016
Bpu10I CCTNAGC 1 cut(s) 901
Bpu14I TTCGAA 1 cut(s) 2489
BpuEI CTTGAG 2 cut(s) 1034, 3452
BpuMI CCSGG 4 cut(s) 34, 104, 1412, 3069
BsaBI GATNNNNATC 2 cut(s) 753, 2694
BsaHI GRCGYC 2 cut(s) 89, 2796
BsaJI CCNNGG 7 cut(s) 1357, 2219, 3117, 3180, 3383, 3551, 3558
BsaWI WCCGGW 4 cut(s) 138, 171, 2849, 3544
BsaXI ACNNNNNCTCC 2 cut(s) 3303, 3333
Bsc4I CCNNNNNNNGG 7 cut(s) 691, 707, 2103, 2345, 2594, 3167, 3551
Bse118I RCCGGY 1 cut(s) 147
Bse1I ACTGG 5 cut(s) 1147, 1481, 1781, 1872, 3232
Bse21I CCTNAGG 2 cut(s) 1766, 1961
Bse3DI GCAATG 4 cut(s) 719, 1878, 2109, 2451
Bse8I GATNNNNATC 2 cut(s) 753, 2694
BseBI CCWGG 6 cut(s) 702, 2739, 2959, 3181, 3384, 3552
BseDI CCNNGG 7 cut(s) 1357, 2219, 3117, 3180, 3383, 3551, 3558
BseJI GATNNNNATC 2 cut(s) 753, 2694
BseLI CCNNNNNNNGG 7 cut(s) 691, 707, 2103, 2345, 2594, 3167, 3551
BseMI GCAATG 4 cut(s) 719, 1878, 2109, 2451
BseMII CTCAG 3 cut(s) 969, 1020, 1509
BseNI ACTGG 5 cut(s) 1147, 1481, 1781, 1872, 3232
BseRI GAGGAG 1 cut(s) 3324
BseXI GCAGC 9 cut(s) 57, 1597, 1668, 2023, 2101, 2443, 3172, 3175, 3279
BseYI CCCAGC 3 cut(s) 84, 737, 1606
BsgI GTGCAG 2 cut(s) 726, 3229
Bsh1236I CGCG 1 cut(s) 1366
Bsh1285I CGRYCG 1 cut(s) 38
BshFI GGCC 8 cut(s) 17, 32, 147, 179, 1017, 1122, 1554, 3063
BshNI GGYRCC 2 cut(s) 2153, 3547
BsiEI CGRYCG 1 cut(s) 38
BsiHKAI GWGCWC 4 cut(s) 171, 550, 1603, 2416
BsiHKCI CYCGRG 1 cut(s) 1379
BslFI GGGAC 4 cut(s) 104, 906, 925, 2080
BslI CCNNNNNNNGG 7 cut(s) 691, 707, 2103, 2345, 2594, 3167, 3551
BsmAI GTCTC 9 cut(s) 15, 355, 363, 383, 439, 447, 869, 1295, 3097
BsmBI CGTCTC 1 cut(s) 15
BsmFI GGGAC 4 cut(s) 104, 906, 925, 2080
BsmI GAATGC 3 cut(s) 989, 2076, 2272
BsnI GGCC 8 cut(s) 17, 32, 147, 179, 1017, 1122, 1554, 3063
BsoBI CYCGRG 1 cut(s) 1379
Bsp119I TTCGAA 1 cut(s) 2489
Bsp1286I GDGCHC 4 cut(s) 171, 550, 1603, 2416
Bsp1407I TGTACA 1 cut(s) 1792
BspACI CCGC 5 cut(s) 79, 122, 180, 190, 1364
BspANI GGCC 8 cut(s) 17, 32, 147, 179, 1017, 1122, 1554, 3063
BspCNI CTCAG 3 cut(s) 970, 1019, 1510
BspFNI CGCG 1 cut(s) 1366
BspHI TCATGA 3 cut(s) 790, 993, 2277
BspMI ACCTGC 4 cut(s) 106, 223, 698, 1221
BspOI GCTAGC 1 cut(s) 2043
BspPI GGATC 7 cut(s) 628, 968, 981, 1470, 1960, 1973, 2315
BspQI GCTCTTC 1 cut(s) 159
BspT104I TTCGAA 1 cut(s) 2489
BspT107I GGYRCC 2 cut(s) 2153, 3547
BspTI CTTAAG 1 cut(s) 2360
BsrBI CCGCTC 1 cut(s) 190
BsrDI GCAATG 4 cut(s) 719, 1878, 2109, 2451
BsrFI RCCGGY 1 cut(s) 147
BsrGI TGTACA 1 cut(s) 1792
BsrI ACTGG 5 cut(s) 1147, 1481, 1781, 1872, 3232
BssAI RCCGGY 1 cut(s) 147
BssECI CCNNGG 7 cut(s) 1357, 2219, 3117, 3180, 3383, 3551, 3558
BssNAI GTATAC 1 cut(s) 2935
BssNI GRCGYC 2 cut(s) 89, 2796
BssSI CACGAG 1 cut(s) 2880
BssT1I CCWWGG 3 cut(s) 1357, 2219, 3558
Bst1107I GTATAC 1 cut(s) 2935
Bst2BI CACGAG 1 cut(s) 2880
Bst2UI CCWGG 6 cut(s) 702, 2739, 2959, 3181, 3384, 3552
Bst6I CTCTTC 9 cut(s) 159, 211, 620, 940, 1287, 2436, 2669, 2949, 3095
BstACI GRCGYC 2 cut(s) 89, 2796
BstAFI CTTAAG 1 cut(s) 2360
BstAUI TGTACA 1 cut(s) 1792
BstBI TTCGAA 1 cut(s) 2489
BstC8I GCNNGC 6 cut(s) 77, 149, 930, 2041, 2583, 2664
BstDSI CCRYGG 1 cut(s) 3117
BstFNI CGCG 1 cut(s) 1366
BstH2I RGCGCY 1 cut(s) 1823
BstHHI GCGC 3 cut(s) 1695, 1822, 2309
BstMAI GTCTC 9 cut(s) 15, 355, 363, 383, 439, 447, 869, 1295, 3097
BstMCI CGRYCG 1 cut(s) 38
BstNI CCWGG 6 cut(s) 702, 2739, 2959, 3181, 3384, 3552
BstNSI RCATGY 3 cut(s) 825, 2585, 2927
BstSFI CTRYAG 2 cut(s) 582, 2340
BstUI CGCG 1 cut(s) 1366
BstV1I GCAGC 9 cut(s) 57, 1597, 1668, 2023, 2101, 2443, 3172, 3175, 3279
BstV2I GAAGAC 1 cut(s) 1028
BstX2I RGATCY 4 cut(s) 633, 973, 1965, 2320
BstXI CCANNNNNNTGG 1 cut(s) 3118
BstYI RGATCY 4 cut(s) 633, 973, 1965, 2320
BstZ17I GTATAC 1 cut(s) 2935
Bsu36I CCTNAGG 2 cut(s) 1766, 1961
BsuRI GGCC 8 cut(s) 17, 32, 147, 179, 1017, 1122, 1554, 3063
BtgI CCRYGG 1 cut(s) 3117
BtrI CACGTC 1 cut(s) 130
BtsI GCAGTG 1 cut(s) 548
BtsIMutI CAGTG 8 cut(s) 548, 873, 1764, 1788, 2090, 2728, 3225, 3225
BveI ACCTGC 4 cut(s) 106, 223, 698, 1221
Cac8I GCNNGC 6 cut(s) 77, 149, 930, 2041, 2583, 2664
CciI TCATGA 3 cut(s) 790, 993, 2277
CfoI GCGC 3 cut(s) 1695, 1822, 2309
Cfr10I RCCGGY 1 cut(s) 147
Cfr13I GGNCC 9 cut(s) 31, 118, 177, 695, 1160, 1361, 1957, 3332, 3555
CseI GACGC 1 cut(s) 78
Csp6I GTAC 8 cut(s) 236, 1234, 1250, 1354, 1793, 2154, 3204, 3540
CspCI CAANNNNNGTGG 2 cut(s) 124, 159
CviQI GTAC 8 cut(s) 236, 1234, 1250, 1354, 1793, 2154, 3204, 3540
DrdI GACNNNNNNGTC 1 cut(s) 3339
DseDI GACNNNNNNGTC 1 cut(s) 3339
EaeI YGGCCR 4 cut(s) 15, 145, 1120, 1552
Eam1104I CTCTTC 9 cut(s) 159, 211, 620, 940, 1287, 2436, 2669, 2949, 3095
EarI CTCTTC 9 cut(s) 159, 211, 620, 940, 1287, 2436, 2669, 2949, 3095
Ecl136II GAGCTC 2 cut(s) 169, 1601
Eco130I CCWWGG 3 cut(s) 1357, 2219, 3558
Eco147I AGGCCT 1 cut(s) 1017
Eco24I GRGCYC 2 cut(s) 171, 1603
Eco32I GATATC 1 cut(s) 1069
Eco47I GGWCC 7 cut(s) 118, 695, 1160, 1361, 1957, 3332, 3555
Eco47III AGCGCT 1 cut(s) 1821
Eco53kI GAGCTC 2 cut(s) 169, 1601
Eco57I CTGAAG 4 cut(s) 1040, 2565, 2687, 3432
Eco81I CCTNAGG 2 cut(s) 1766, 1961
Eco88I CYCGRG 1 cut(s) 1379
EcoICRI GAGCTC 2 cut(s) 169, 1601
EcoO109I RGGNCCY 3 cut(s) 118, 695, 1957
EcoRI GAATTC 1 cut(s) 2285
EcoRII CCWGG 6 cut(s) 700, 2737, 2957, 3179, 3382, 3550
EcoRV GATATC 1 cut(s) 1069
EcoT14I CCWWGG 3 cut(s) 1357, 2219, 3558
EcoT22I ATGCAT 1 cut(s) 991
EcoT38I GRGCYC 2 cut(s) 171, 1603
ErhI CCWWGG 3 cut(s) 1357, 2219, 3558
Esp3I CGTCTC 1 cut(s) 15
FalI AAGNNNNNCTT 6 cut(s) 1480, 1512, 2343, 2375, 2685, 2717
FaqI GGGAC 4 cut(s) 104, 906, 925, 2080
FauI CCCGC 1 cut(s) 129
FbaI TGATCA 1 cut(s) 2689
FblI GTMKAC 2 cut(s) 38, 2934
FriOI GRGCYC 2 cut(s) 171, 1603
FspBI CTAG 6 cut(s) 675, 915, 1026, 2040, 3056, 3338
GlaI GCGC 3 cut(s) 1694, 1821, 2308
GsaI CCCAGC 3 cut(s) 88, 741, 1610
GsuI CTGGAG 2 cut(s) 684, 2016
HaeII RGCGCY 1 cut(s) 1823
HaeIII GGCC 8 cut(s) 17, 32, 147, 179, 1017, 1122, 1554, 3063
HgaI GACGC 1 cut(s) 78
HhaI GCGC 3 cut(s) 1695, 1822, 2309
Hin1I GRCGYC 2 cut(s) 89, 2796
Hin6I GCGC 3 cut(s) 1693, 1820, 2307
HinP1I GCGC 3 cut(s) 1693, 1820, 2307
HincII GTYRAC 2 cut(s) 39, 839
HindII GTYRAC 2 cut(s) 39, 839
HindIII AAGCTT 3 cut(s) 743, 3279, 3356
HphI GGTGA 4 cut(s) 1103, 1595, 2152, 2910
Hpy99I CGWCG 1 cut(s) 43
HpyCH4IV ACGT 2 cut(s) 129, 2796
HpySE526I ACGT 2 cut(s) 129, 2796
Hsp92I GRCGYC 2 cut(s) 89, 2796
HspAI GCGC 3 cut(s) 1693, 1820, 2307
KpnI GGTACC 1 cut(s) 2157
KroI GCCGGC 1 cut(s) 147
KroNI GCCGGC 1 cut(s) 149
Ksp22I TGATCA 1 cut(s) 2689
LguI GCTCTTC 1 cut(s) 159
Lsp1109I GCAGC 9 cut(s) 57, 1597, 1668, 2023, 2101, 2443, 3172, 3175, 3279
LweI GCATC 8 cut(s) 213, 517, 887, 976, 1574, 3040, 3166, 3417
MaeI CTAG 6 cut(s) 675, 915, 1026, 2040, 3056, 3338
MaeII ACGT 2 cut(s) 129, 2796
MbiI CCGCTC 1 cut(s) 190
MfeI CAATTG 2 cut(s) 2271, 3461
MflI RGATCY 4 cut(s) 633, 973, 1965, 2320
MhlI GDGCHC 4 cut(s) 171, 550, 1603, 2416
MlsI TGGCCA 2 cut(s) 1122, 1554
MluNI TGGCCA 2 cut(s) 1122, 1554
MlyI GAGTC 3 cut(s) 1765, 1994, 2686
MmeI TCCRAC 3 cut(s) 221, 1138, 2638
Mox20I TGGCCA 2 cut(s) 1122, 1554
Mph1103I ATGCAT 1 cut(s) 991
MroNI GCCGGC 1 cut(s) 147
MroXI GAANNNNTTC 5 cut(s) 351, 435, 2285, 2943, 3378
MscI TGGCCA 2 cut(s) 1122, 1554
MslI CAYNNNNRTG 2 cut(s) 3116, 3197
Msp20I TGGCCA 2 cut(s) 1122, 1554
MspA1I CMGCKG 3 cut(s) 1610, 2036, 2666
MspCI CTTAAG 1 cut(s) 2360
MunI CAATTG 2 cut(s) 2271, 3461
Mva1269I GAATGC 3 cut(s) 989, 2076, 2272
MvaI CCWGG 6 cut(s) 702, 2739, 2959, 3181, 3384, 3552
MvnI CGCG 1 cut(s) 1366
NaeI GCCGGC 1 cut(s) 149
NciI CCSGG 4 cut(s) 34, 104, 1412, 3069
NgoMIV GCCGGC 1 cut(s) 147
NheI GCTAGC 1 cut(s) 2039
NmuCI GTSAC 4 cut(s) 1033, 1135, 1264, 3388
NsiI ATGCAT 1 cut(s) 991
NspI RCATGY 3 cut(s) 825, 2585, 2927
NspV TTCGAA 1 cut(s) 2489
PaeI GCATGC 1 cut(s) 2585
PaeR7I CTCGAG 1 cut(s) 1379
PagI TCATGA 3 cut(s) 790, 993, 2277
PceI AGGCCT 1 cut(s) 1017
PciI ACATGT 2 cut(s) 821, 2923
PciSI GCTCTTC 1 cut(s) 159
PcsI WCGNNNNNNNCGW 1 cut(s) 2289
PctI GAATGC 3 cut(s) 989, 2076, 2272
PdiI GCCGGC 1 cut(s) 149
PdmI GAANNNNTTC 5 cut(s) 351, 435, 2285, 2943, 3378
PflMI CCANNNNNTGG 1 cut(s) 3167
PfoI TCCNGGA 2 cut(s) 102, 3067
PleI GAGTC 3 cut(s) 1765, 1993, 2685
PpsI GAGTC 3 cut(s) 1765, 1993, 2685
PpuMI RGGWCCY 3 cut(s) 118, 695, 1957
PscI ACATGT 2 cut(s) 821, 2923
PshBI ATTAAT 2 cut(s) 1839, 2331
PsiI TTATAA 1 cut(s) 1902
Psp124BI GAGCTC 2 cut(s) 171, 1603
Psp5II RGGWCCY 3 cut(s) 118, 695, 1957
Psp6I CCWGG 6 cut(s) 700, 2737, 2957, 3179, 3382, 3550
PspFI CCCAGC 3 cut(s) 84, 737, 1606
PspGI CCWGG 6 cut(s) 700, 2737, 2957, 3179, 3382, 3550
PspPI GGNCC 9 cut(s) 31, 118, 177, 695, 1160, 1361, 1957, 3332, 3555
PspPPI RGGWCCY 3 cut(s) 118, 695, 1957
PsrI GAACNNNNNNTAC 2 cut(s) 764, 796
PsuI RGATCY 4 cut(s) 633, 973, 1965, 2320
PvuII CAGCTG 3 cut(s) 1610, 2036, 2666
RsaI GTAC 8 cut(s) 237, 1235, 1251, 1355, 1794, 2155, 3205, 3541
RsaNI GTAC 8 cut(s) 236, 1234, 1250, 1354, 1793, 2154, 3204, 3540
RseI CAYNNNNRTG 2 cut(s) 3116, 3197
SacI GAGCTC 2 cut(s) 171, 1603
SalI GTCGAC 1 cut(s) 37
SapI GCTCTTC 1 cut(s) 159
Sau96I GGNCC 9 cut(s) 31, 118, 177, 695, 1160, 1361, 1957, 3332, 3555
ScaI AGTACT 1 cut(s) 3541
SchI GAGTC 3 cut(s) 1765, 1994, 2686
SduI GDGCHC 4 cut(s) 171, 550, 1603, 2416
SfaNI GCATC 8 cut(s) 213, 517, 887, 976, 1574, 3040, 3166, 3417
SfcI CTRYAG 2 cut(s) 582, 2340
Sfr274I CTCGAG 1 cut(s) 1379
SfuI TTCGAA 1 cut(s) 2489
SinI GGWCC 7 cut(s) 118, 695, 1160, 1361, 1957, 3332, 3555
SlaI CTCGAG 1 cut(s) 1379
SmiMI CAYNNNNRTG 2 cut(s) 3116, 3197
SmlI CTYRAG 4 cut(s) 1049, 1379, 2360, 3431
SmoI CTYRAG 4 cut(s) 1049, 1379, 2360, 3431
SpeI ACTAGT 1 cut(s) 1025
SphI GCATGC 1 cut(s) 2585
SseBI AGGCCT 1 cut(s) 1017
SsiI CCGC 5 cut(s) 79, 122, 180, 190, 1364
SspI AATATT 2 cut(s) 2390, 3368
SspMI CTAG 6 cut(s) 675, 915, 1026, 2040, 3056, 3338
SstI GAGCTC 2 cut(s) 171, 1603
StuI AGGCCT 1 cut(s) 1017
StyI CCWWGG 3 cut(s) 1357, 2219, 3558
TaiI ACGT 2 cut(s) 132, 2799
TaqI TCGA 8 cut(s) 38, 186, 240, 515, 1380, 2292, 2489, 2655
TatI WGTACW 2 cut(s) 1792, 3539
TauI GCSGC 2 cut(s) 182, 193
TscAI CASTG 8 cut(s) 555, 873, 1764, 1788, 2097, 2728, 3225, 3232
TseFI GTSAC 4 cut(s) 1033, 1135, 1264, 3388
TseI GCWGC 9 cut(s) 45, 1610, 1681, 2036, 2114, 2456, 3185, 3188, 3292
Tsp45I GTSAC 4 cut(s) 1033, 1135, 1264, 3388
TspGWI ACGGA 2 cut(s) 537, 2954
TspRI CASTG 8 cut(s) 555, 873, 1764, 1788, 2097, 2728, 3225, 3232
Van91I CCANNNNNTGG 1 cut(s) 3167
Vha464I CTTAAG 1 cut(s) 2360
VpaK11BI GGWCC 7 cut(s) 118, 695, 1160, 1361, 1957, 3332, 3555
VspI ATTAAT 2 cut(s) 1839, 2331
XapI RAATTY 3 cut(s) 1188, 1808, 2285
XceI RCATGY 3 cut(s) 825, 2585, 2927
XhoI CTCGAG 1 cut(s) 1379
XmiI GTMKAC 2 cut(s) 38, 2934
XmnI GAANNNNTTC 5 cut(s) 351, 435, 2285, 2943, 3378
XspI CTAG 6 cut(s) 675, 915, 1026, 2040, 3056, 3338
ZraI GACGTC 1 cut(s) 2797
ZrmI AGTACT 1 cut(s) 3541
Zsp2I ATGCAT 1 cut(s) 991
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.