MD14G1017900.v1.1
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0000245
Physical Location & Seq
Reverse (-)
1671255 .. 1677967
6713 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1017900.v1.1.491

Sequence Viewer

Length: 4431 bp
ATGGCGGCGGCTTTGGCGGAGGACGATATGGCTCGGTCGACAAGCCAGAGGAGCTGGGGGTCGGGGAGCGAGCGGAGTTGGGCATCCACGAGTTTCCGGGAAGTGTGGCAGGCACCACCGGATGTGTTCAACAGAAGCAGGCGGCAGGACGACGAGGAGGAGCTGCGGTGGGCGGCAATCCAACGGCTGCCGACGTATGACAGGTTGAAAAGAGGGATGCTGAGGCAGGTGCTGGATAACGGGAAGGTGGTGACTGATGAGGTGGACGTGACGAGGCTGGGAATGCAGGACAAGAAGCAGTTGATGGAGAGTATACTCAAGGTTGTGGAAGATGATAACGAAAAGTTCTTGAGAAGACTTAGAGACAGAACCGATAGACGGGATTCAAACTCGGGTGCATGGGCTGGTCACACTGCCCTACTCAACTCTACGAAACCTTGTCTTCAAAACCCAATGGGGATTGAAATTCCGAAGATTGAAGTTCGATACGAGAATTTGTCGATTGAAGGAGATGTGTATGTTGGGAGCAGAGCACTGCCTACTCTGCTCAGTGCTACCTTAAACACTATTGAGAGTGTTCTGGGATTAATTAAGCTTGCTCCATCAAAAAAGAGAAAGATCCAGATACTTAAAGACGTTAGTGGAATTGTCAGACCTTCCAGGATGACCCTACTTTTGGGTCCTCCAGGTGCAGGCAAAACAACCTTGTTACTAGCACTTGCAGGAAAGCTTGATGATGATCTAAGAGTTTCTGGGAAAATCACATATTGTGGCCATGAACTGAATGAGTTTGTTCCAAAAAGAACATGTGCTTACATTAGTCAGCATGATCTTCACTATGGAGAAATGACAGTGAGAGAGACGTTGGATTTCTCAGCACGATGTTTGGGTGTCGGAACTAGATACCAAATGCTGGCTGAACTCTCAAGAAGAGAGAAAGAAGCCGGAATCAAGCCTGACCCCGAGATTGATGCATTCATGAAGGCCACCTCAGTCTCAGGCCAGAAGACTAGTTTGGTCACAGATTATGTTCTCAAGATACTTGGATTGGATATCTGTGCTGATATCATGGTTGGTAATGATATGAGAAGGGGTATTTCCGGTGGCCAGAAGAAGCGCGTGACCACCGGGGAGATGTTGGTAGGACCAGCAAAGGTTCTTCTGATGGATGAAATATCAACAGGGTTAGACAGTTCCACAACTTTTCAGATATGCAGATACACACGGCAATTGGTTCACATAATGGATGTAACCATGGTCATCTCTCTTCTGCAGCCCGCGCCGGAGACATTTGAGCTCTTCGATGACCTAATCCTGCTTTCGGAAGGACAGATTGTGTACCAAGGTCCATGCGAGAGTGTCCTCGAGTTCTTCGAACACGTGGGATTCAAGTGCCCAGAGAGGAAAGGAGTTGCCGATTTTTTGCAAGAGGTGACATCCAAGAAGGACCAAGAACAATATTGGTTAAAAAAAAGCCAGCCCTACAGATATATCTCGGTTCCTGAGTTCGTGGAGGCCTTCAGCTCTTTTAGGAGTGGCCAGCAACTTGCAGCTGATCTTGGGGTTCCTTACGACAAGTCCAGAGCTCATCCGGCTGCATTGGTCACAGATAAGTATGGCATTTCAAACTGGGAATTGTTCAAGGCATGTTTTTCGAGGGAGTGGCTGCTAATGAAGCGCAATTCGTTTGTGTACATATTCAAGACCACGCAGATTGCAATCATGTCTTTGATTGCCTTCACTGTGTTCCTTAGAACAGAAATGCCTGTGGGCACAATACAAAATGGAGGAAAATTTTTTGGAGCGTTGTTCTTCAGTCTGATTAATGTGATGTTGAATGGTATGGCGGAACTGGGAATGACTGTTTTCAGACTTCCTGTTTTTTTTAAGCAGAGGGATTTCTTGTTTTATCCTGCGTGGGCTTTCGGGTTACCAATTTGGGTCCTCAGGATCCCACTTTCGTTCGTGGAATCAGGGATTTGGATCATTCTTACCTATTACACCATTGGATTTGCTCCATCTGCTAGCAGATTTTTCAAACAGTTCTTGGCATTCTTTGGCATACATCAGATGGCTCTGTCCCTCTTTCGGTTTATTGCTGCACTTGGAAGAACACAAGTTGTTGCTAGCACGCTCGGTACCTTTACCTTGCTAATGGCATTTGTCCTGGGAGGGTTTATTGTTGCCAAAAATGACCTTGAGCCTTGGATGCTATGGGGCTACTACATTTCTCCCATGATGTATGGACAGAATGCGATCGTCATGAATGAATTCCTTGACAAAAGATGGAGCGCGCCAAATACAGATCCAAGAATTAACGCAACTACGGTCGGGAAAGTCCTTCTCAAGTCTAGAGGATTCTTCACAGATGAATACTGGTTTTGGATATGTATTGGAGCACTATTTGGTTTTTCTCTTCTCTTCAACATCTTGTTCATTGCAGCATTGACTTTTCTGAACCCTCTCGGGGATGCGAAAGCTGTCATAGCTGATGATGAAAGTGAAGGAAAGAGAAGCAAGTCGTTAAGTGAAGAAATGACAGCAAAAAGTTCTTCAGAAATTGCTGGTGCTTCGGATCATGCACCAAAGAAAGGAATGGTTTTGCCCTTCCAACCCCTTTCGCTTGCGTTTAACCATGTGAACTACTTTGTGGATATGCCCCCTGAAATGAAGACTCATGGAGTTGAAGAAGATCGCCTTCAATTGCTACGAGATGTCAGTGGTGCTTTTAGACCGGGAATATTGACAGCGCTTGTAGGAGTTAGTGGTGCTGGAAAGACAACTCTTATGGACGTCCTGGCAGGTAGAAAGACTGGTGGATATATCGAAGGAACCATTAACATATCTGGTTATCCAAAGAACCAAGAAACATTTGCTCGTGTCAGCGGTTATTGTGAACAGAATGACATCCATTCCCCACATGTTACTGTCTATGAATCTCTCCTGTATTCAGCCTGGCTCCGTCTTGCTTCTGATGTCAGTACACAAACACGAAAGATGTTTGTTGAAGAAGTCATGGATTTGGTTGAGCTTAATCCAATAAGAAATTCTCTCGTTGGCCTCCCGGGACTAGATGGACTTTCAACAGAACAACGGAAGAGACTAACCATAGCTGTGGAGCTGGTCGCTGACCCCTCTATCATTTTCATGGATGAACCGACATCTGGTCTCGATGCCAGGGCTGCTGCTATCGTCATGCGTACTGTGAGAAACACGGTGGACACAGGCAGAACTGTTGTCTGCACAATTCACCAGCCAAGCATAGATATTTTTGAATCTTTCGATGAGTTACTCTTGATGAAACGTGGAGGGCAAGTCATTTATGCTGGACCCCTCAGCCGCCACTCTCACAAGCTTGTGGAATATTTTGAAGCCATCCCGGGTGTGCCCAAGATCAAGGATGGCTACAATCCTGCTACATGGATGCTTGAGGTCACTGCTCCTGCAGTTGAGACTCAACTTGATGTGGACTTTGCAGACATTTACCAAAACTCCTCGCTTTACCAGAGGAACCAGGAGCTTATCAAAGAGCTTAGTACACCGGCTCCCGGGTCCAAAGATCTCTACTTTCCAACCAAATACTCTCAACCTTTCCCGGTTCAGTGCAAAGCTTGTTTCTGGAAAATGCATTGGTCTTATTGGAGAAATCCTCAGTACAATGCCATCCGATTCTTTATGACCATTGTCATCGGTGTCTTATTCGGTCTTATCTTCTGGCAGAAGGGACAGCAGACAGCACAACAGCAAGATCTAATGAACCTTTTGGGAGCTATGTATGCTGCTGTGCTTTTCCTTGGAACCACGAATGCTTCTGTGGTCCGATTTGTTGTCGCCATTGAAAGAACAGTTTTCTACCGCGAAAGAGCTGCCGGAATGTATTCTGAACTGCCTTATGCATTTGCTCAGGTGGCTATAGAGACAATCTATGTTGCAATCCAAACATTTATATATACACTTCTTCTCTTCTCCATGATTGGATTCAAGTGGAAAGTCGAAAAATTCATTTGGTTCTACTACTATATCCTAATGTGCTTCATCTACTTCACGATGTACGGTATGATGGTGGTTGCACTGACTCCGGGCCACCAGATTGCTGTCATTGTCATGCCCTTCTTCCTCAGTTTCTGGAACTTGTTTTCCGGCTTCCTCATTCCAAGACCGCAAATACCAATCTGGTGGAGGTGGTACTACTGGGCTTCCCCTGTGGCCTGGACACTTTATGGCCTTGTGACATCCCAAGTGGGTGACAAGAATGCTGACCTTATCCTGCCTGGATATGGCACCATGCCATTGAAGAAGTTCCTGAAAGATGACTTTGGTTTTGAGTATGACTTCCTTCCGGCCGTCGCCGCCGCACACGTCGGCTGGGTTCTTCTTTTCTTCTTTGTGTTTGCCTATGGCATCAAGTTCCTCAACTTCCAAAGGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1477

Amino Acids

167.02

Weight (kDa)

8.21

Isoelectric Point (pI)

38.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 210 - 392 4.2e-17 ABC transporter
ABC2_membrane_7 PF19055 425 - 477 1e-06 ABC-2 type transporter
ABC2_membrane PF01061 546 - 758 5.5e-44 ABC-2 type transporter
PDR_assoc PF08370 763 - 829 1.4e-27 Plant PDR ABC transporter associated
ABC_tran PF00005 903 - 1055 8e-21 ABC transporter
ABC2_membrane PF01061 1201 - 1413 4.2e-56 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000245)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66950 AT2G36380
fragaria_vesca FvH4_3g29700 FvH4_3g29700 FvH4_3g29700 FvH4_3g29700 FvH4_3g29710 FvH4_3g29740 FvH4_3g29740 FvH4_3g29740 FvH4_3g29740 FvH4_3g29740 FvH4_3g29760 FvH4_3g29781 FvH4_6g18150 FvH4_6g18150 FvH4_6g18150 FvH4_6g18150 FvH4_6g18150
malus_domestica MD02G1254900.v1.1 MD03G1119400.v1.1 MD03G1136800.v1.1 MD03G1137000.v1.1 MD04G1187500.v1.1 MD08G1208900.v1.1 MD08G1209000.v1.1 MD11G1159000.v1.1 MD11G1159100.v1.1 MD11G1159200.v1.1 MD12G1020800.v1.1 MD12G1021000.v1.1 MD14G1017900.v1.1 MD14G1018100.v1.1
prunus_persica Prupe.2G084300_v2.0.a1 Prupe.2G084300_v2.0.a1 Prupe.6G121200_v2.0.a1 Prupe.6G121200_v2.0.a1 Prupe.6G121500_v2.0.a1 Prupe.6G121800_v2.0.a1 Prupe.6G121900_v2.0.a1 Prupe.6G122100_v2.0.a1 Prupe.7G115200_v2.0.a1 Prupe.7G115200_v2.0.a1
pyrus_communis pycom02g21460 pycom03g09270 pycom03g09290 pycom07g05130 pycom08g18050 pycom11g12950 pycom11g12960 pycom11g12970 pycom12g01950 pycom12g01980 pycom14g01620 pycom14g01710 pycom14g01720 pycom16g12190
rosa_chinensis RchiOBHm_Chr1g0340571 RchiOBHm_Chr2g0107751 RchiOBHm_Chr3g0472451 RchiOBHm_Chr5g0055051 RchiOBHm_Chr5g0055091 RchiOBHm_Chr5g0055111 RchiOBHm_Chr5g0055121 RchiOBHm_Chr5g0055131 RchiOBHm_Chr5g0062371 RchiOBHm_Chr6g0265121 RchiOBHm_Chr6g0275771 RchiOBHm_Chr7g0187231
rosa_laevigata RLG00000006319 RLG00000013820 RLG00000024078 RLG00000034960 RLG00000034963 RLG00000034965 RLG00000034966 RLG00000034968 RLG00000034970
rosa_multiflora Rmu_co8358859.1_g000001 Rmu_co8422375.1_g000001 Rmu_co8427767.1_g000001 Rmu_co8474941.1_g000001 Rmu_sc0000925.1_g000009 Rmu_sc0001694.1_g000015 Rmu_sc0001694.1_g000022 Rmu_sc0001694.1_g000024 Rmu_sc0001694.1_g000030 Rmu_sc0003993.1_g000001 Rmu_sc0010632.1_g000001 Rmu_sc0035175.1_g000001
rosa_roxburghii Rroxscaffold_1G00025230 Rroxscaffold_1G00025240 Rroxscaffold_1G00025300 Rroxscaffold_1G00025320 Rroxscaffold_1G00025330 Rroxscaffold_1G00025360 Rroxscaffold_1G00025440 Rroxscaffold_1G00033590 Rroxscaffold_6G00408900
rosa_rugosa Rorug03G0125500.1 Rorug03G0125600.1 Rorug05G0290600 Rorug05G0291100 Rorug05G0291200 Rorug05G0291300
rosa_samantha Rh1CG035400 Rh1CG243200 Rh2DG410600 Rh3AG177500 Rh3CG193800 Rh3CG200900 Rh3DG171700 Rh5AG043000 Rh5AG360500 Rh5AG360900 Rh5AG361000 Rh5AG361300 Rh5AG441100 Rh5CG393700 Rh5CG393900 Rh5CG394200 Rh5DG247300 Rh5DG385200 Rh5DG385500 Rh5DG385800 Rh6BG272200 Rh6DG204000 Rh7AG084700 Rh7AG178300
rosa_wichuraiana Rw0G022120 Rw3G016410 Rw5G033910 Rw5G033920 Rw5G033940 Rw5G033960 Rw5G033970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 217
AatII GACGTC 1 cut(s) 2796
Acc36I ACCTGC 2 cut(s) 217, 2792
Acc65I GGTACC 1 cut(s) 2138
AccB1I GGYRCC 3 cut(s) 112, 2138, 4280
AccB7I CCANNNNNTGG 1 cut(s) 913
AccBSI CCGCTC 1 cut(s) 73
AccI GTMKAC 2 cut(s) 38, 313
AccII CGCG 4 cut(s) 1119, 1280, 2294, 3858
AclWI GGATC 6 cut(s) 613, 1945, 1958, 1991, 2300, 2583
AcoI YGGCCR 4 cut(s) 772, 1105, 1537, 4341
AcsI RAATTY 6 cut(s) 465, 493, 1793, 2270, 3046, 3998
AcuI CTGAAG 3 cut(s) 1504, 1798, 2538
AcvI CACGTG 1 cut(s) 1381
AcyI GRCGYC 1 cut(s) 2793
AfaI GTAC 9 cut(s) 1340, 1694, 2140, 2983, 3202, 3538, 3656, 4052, 4187
AfeI AGCGCT 1 cut(s) 2751
AflIII ACRYGT 4 cut(s) 806, 1378, 2920, 4357
AhdI GACNNNNNGTC 1 cut(s) 3165
AhlI ACTAGT 1 cut(s) 1010
AjiI CACGTC 2 cut(s) 268, 4360
AjnI CCWGG 9 cut(s) 659, 685, 2166, 2796, 2954, 3176, 3513, 4208, 4270
AjuI GAANNNNNNNTTGG 4 cut(s) 998, 1030, 3031, 3063
AloI GAACNNNNNNTCC 2 cut(s) 4121, 4153
Alw21I GWGCWC 4 cut(s) 535, 1299, 1588, 2401
Alw26I GTCTC 8 cut(s) 357, 854, 1000, 1280, 3094, 3173, 3446, 3911
AlwI GGATC 6 cut(s) 613, 1945, 1958, 1991, 2300, 2583
AlwNI CAGNNNCTG 2 cut(s) 232, 4125
Ama87I CYCGRG 7 cut(s) 391, 962, 1364, 2465, 3064, 3379, 3548
Aor51HI AGCGCT 1 cut(s) 2751
ApoI RAATTY 6 cut(s) 465, 493, 1793, 2270, 3046, 3998
ArsI GACNNNNNNTTYG 4 cut(s) 658, 690, 4304, 4336
AseI ATTAAT 2 cut(s) 587, 1824
Asp700I GAANNNNTTC 3 cut(s) 2270, 3877, 4298
Asp718I GGTACC 1 cut(s) 2138
AspLEI GCGC 6 cut(s) 1119, 1282, 1680, 2294, 2296, 2752
AspS9I GGNCC 9 cut(s) 680, 1145, 1346, 1447, 1942, 3329, 3552, 3817, 4081
AsuHPI GGTGA 4 cut(s) 262, 1444, 3242, 4256
AsuII TTCGAA 1 cut(s) 1374
AsuNHI GCTAGC 2 cut(s) 2024, 2126
AvaI CYCGRG 7 cut(s) 391, 962, 1364, 2465, 3064, 3379, 3548
AvaII GGWCC 8 cut(s) 680, 1145, 1346, 1447, 1942, 3329, 3552, 3817
AxyI CCTNAGG 1 cut(s) 1946
BaeGI GKGCMC 3 cut(s) 1397, 1775, 3390
BalI TGGCCA 3 cut(s) 774, 1107, 1539
BamHI GGATCC 1 cut(s) 1950
BanI GGYRCC 3 cut(s) 112, 2138, 4280
BanII GRGCYC 2 cut(s) 1299, 1588
BauI CACGAG 2 cut(s) 88, 2877
BbrPI CACGTG 1 cut(s) 1381
BbsI GAAGAC 4 cut(s) 361, 434, 1013, 2678
Bbv12I GWGCWC 4 cut(s) 535, 1299, 1588, 2401
BbvCI CCTCAGC 2 cut(s) 221, 3335
BceAI ACGGC 3 cut(s) 200, 1241, 4328
BcgI CGANNNNNNTGC 6 cut(s) 953, 987, 1635, 1669, 3848, 3882
BciT130I CCWGG 9 cut(s) 661, 687, 2168, 2798, 2956, 3178, 3515, 4210, 4272
BcoDI GTCTC 8 cut(s) 357, 854, 1000, 1280, 3094, 3173, 3446, 3911
BcuI ACTAGT 1 cut(s) 1010
BfaI CTAG 7 cut(s) 713, 900, 1011, 2025, 2127, 2352, 3071
BfmI CTRYAG 4 cut(s) 1271, 1483, 3444, 3912
BfoI RGCGCY 1 cut(s) 2753
BfuAI ACCTGC 2 cut(s) 217, 2792
BglII AGATCT 2 cut(s) 3559, 3748
Bme18I GGWCC 8 cut(s) 680, 1145, 1346, 1447, 1942, 3329, 3552, 3817
BmeRI GACNNNNNGTC 1 cut(s) 3165
BmeT110I CYCGRG 7 cut(s) 391, 962, 1364, 2465, 3064, 3379, 3548
BmgBI CACGTC 2 cut(s) 268, 4360
BmgT120I GGNCC 9 cut(s) 680, 1145, 1346, 1447, 1942, 3329, 3552, 3817, 4081
BmrI ACTGGG 3 cut(s) 1639, 1862, 4201
BmsI GCATC 8 cut(s) 92, 207, 961, 2199, 2461, 3163, 3414, 4410
BmtI GCTAGC 2 cut(s) 2028, 2130
BmuI ACTGGG 3 cut(s) 1639, 1862, 4201
BoxI GACNNNNGTC 1 cut(s) 3683
BpiI GAAGAC 4 cut(s) 361, 434, 1013, 2678
BplI GAGNNNNNCTC 2 cut(s) 3634, 3666
BpmI CTGGAG 1 cut(s) 669
Bpu10I CCTNAGC 3 cut(s) 221, 3335, 3903
Bpu14I TTCGAA 1 cut(s) 1374
BpuEI CTTGAG 7 cut(s) 302, 370, 910, 1019, 2219, 2330, 3449
BsaAI YACGTR 1 cut(s) 1381
BsaBI GATNNNNATC 3 cut(s) 738, 1718, 1982
BsaHI GRCGYC 1 cut(s) 2793
BsaI GGTCTC 1 cut(s) 3173
BsaWI WCCGGW 2 cut(s) 118, 1100
BsaXI ACNNNNNCTCC 8 cut(s) 152, 182, 3300, 3330, 3476, 3506, 3529, 3559
Bse118I RCCGGY 1 cut(s) 3541
Bse1I ACTGG 5 cut(s) 1634, 1857, 2381, 2818, 4196
Bse21I CCTNAGG 1 cut(s) 1946
Bse3DI GCAATG 1 cut(s) 2436
Bse8I GATNNNNATC 3 cut(s) 738, 1718, 1982
BseBI CCWGG 9 cut(s) 661, 687, 2168, 2798, 2956, 3178, 3515, 4210, 4272
BseJI GATNNNNATC 3 cut(s) 738, 1718, 1982
BseMI GCAATG 1 cut(s) 2436
BseNI ACTGG 5 cut(s) 1634, 1857, 2381, 2818, 4196
BsePI GCGCGC 1 cut(s) 2292
BseRI GAGGAG 4 cut(s) 64, 170, 173, 3484
BseSI GKGCMC 3 cut(s) 1397, 1775, 3390
BseX3I CGGCCG 1 cut(s) 4341
BseYI CCCAGC 3 cut(s) 54, 277, 4365
BsgI GTGCAG 3 cut(s) 711, 2085, 3226
Bsh1236I CGCG 4 cut(s) 1119, 1280, 2294, 3858
Bsh1285I CGRYCG 4 cut(s) 38, 2259, 2331, 4344
BshNI GGYRCC 3 cut(s) 112, 2138, 4280
BsiEI CGRYCG 4 cut(s) 38, 2259, 2331, 4344
BsiHKAI GWGCWC 4 cut(s) 535, 1299, 1588, 2401
BsiHKCI CYCGRG 7 cut(s) 391, 962, 1364, 2465, 3064, 3379, 3548
BslFI GGGAC 3 cut(s) 2065, 3081, 3738
BsmAI GTCTC 8 cut(s) 357, 854, 1000, 1280, 3094, 3173, 3446, 3911
BsmBI CGTCTC 1 cut(s) 854
BsmFI GGGAC 3 cut(s) 2065, 3081, 3738
BsmI GAATGC 6 cut(s) 288, 974, 2051, 2257, 3811, 4258
Bso31I GGTCTC 1 cut(s) 3173
BsoBI CYCGRG 7 cut(s) 391, 962, 1364, 2465, 3064, 3379, 3548
Bsp119I TTCGAA 1 cut(s) 1374
Bsp1286I GDGCHC 7 cut(s) 535, 1299, 1397, 1588, 1775, 2401, 3390
Bsp1407I TGTACA 1 cut(s) 1692
Bsp19I CCATGG 1 cut(s) 1254
BspFNI CGCG 4 cut(s) 1119, 1280, 2294, 3858
BspHI TCATGA 2 cut(s) 978, 2262
BspMAI CTGCAG 2 cut(s) 1275, 3448
BspMI ACCTGC 2 cut(s) 217, 2792
BspOI GCTAGC 2 cut(s) 2028, 2130
BspPI GGATC 6 cut(s) 613, 1945, 1958, 1991, 2300, 2583
BspQI GCTCTTC 1 cut(s) 1304
BspT104I TTCGAA 1 cut(s) 1374
BspT107I GGYRCC 3 cut(s) 112, 2138, 4280
BspTNI GGTCTC 1 cut(s) 3173
BsrBI CCGCTC 1 cut(s) 73
BsrDI GCAATG 1 cut(s) 2436
BsrFI RCCGGY 1 cut(s) 3541
BsrGI TGTACA 1 cut(s) 1692
BsrI ACTGG 5 cut(s) 1634, 1857, 2381, 2818, 4196
BssAI RCCGGY 1 cut(s) 3541
BssHII GCGCGC 1 cut(s) 2292
BssNAI GTATAC 1 cut(s) 314
BssNI GRCGYC 1 cut(s) 2793
BssSI CACGAG 2 cut(s) 88, 2877
BssT1I CCWWGG 4 cut(s) 1254, 1342, 2204, 3793
Bst1107I GTATAC 1 cut(s) 314
Bst2BI CACGAG 2 cut(s) 88, 2877
Bst2UI CCWGG 9 cut(s) 661, 687, 2168, 2798, 2956, 3178, 3515, 4210, 4272
Bst6I CTCTTC 7 cut(s) 925, 1272, 1304, 2421, 2426, 3092, 3968
BstACI GRCGYC 1 cut(s) 2793
BstAUI TGTACA 1 cut(s) 1692
BstBAI YACGTR 1 cut(s) 1381
BstBI TTCGAA 1 cut(s) 1374
BstDSI CCRYGG 1 cut(s) 1254
BstEII GGTNACC 1 cut(s) 1929
BstFNI CGCG 4 cut(s) 1119, 1280, 2294, 3858
BstH2I RGCGCY 1 cut(s) 2753
BstHHI GCGC 6 cut(s) 1119, 1282, 1680, 2294, 2296, 2752
BstMAI GTCTC 8 cut(s) 357, 854, 1000, 1280, 3094, 3173, 3446, 3911
BstMCI CGRYCG 4 cut(s) 38, 2259, 2331, 4344
BstNI CCWGG 9 cut(s) 661, 687, 2168, 2798, 2956, 3178, 3515, 4210, 4272
BstNSI RCATGY 3 cut(s) 810, 1650, 2924
BstPAI GACNNNNGTC 1 cut(s) 3683
BstPI GGTNACC 1 cut(s) 1929
BstSFI CTRYAG 4 cut(s) 1271, 1483, 3444, 3912
BstSLI GKGCMC 3 cut(s) 1397, 1775, 3390
BstUI CGCG 4 cut(s) 1119, 1280, 2294, 3858
BstV2I GAAGAC 4 cut(s) 361, 434, 1013, 2678
BstX2I RGATCY 5 cut(s) 618, 1950, 2305, 3559, 3748
BstXI CCANNNNNNTGG 2 cut(s) 3115, 4176
BstYI RGATCY 5 cut(s) 618, 1950, 2305, 3559, 3748
BstZ17I GTATAC 1 cut(s) 314
BstZI CGGCCG 1 cut(s) 4341
Bsu36I CCTNAGG 1 cut(s) 1946
BtgI CCRYGG 1 cut(s) 1254
BtrI CACGTC 2 cut(s) 268, 4360
BtsI GCAGTG 3 cut(s) 411, 533, 3435
BtsIMutI CAGTG 9 cut(s) 411, 533, 556, 858, 1740, 2725, 3435, 3608, 4070
BveI ACCTGC 2 cut(s) 217, 2792
CaiI CAGNNNCTG 2 cut(s) 232, 4125
CciI TCATGA 2 cut(s) 978, 2262
CfoI GCGC 6 cut(s) 1119, 1282, 1680, 2294, 2296, 2752
Cfr10I RCCGGY 1 cut(s) 3541
Cfr13I GGNCC 9 cut(s) 680, 1145, 1346, 1447, 1942, 3329, 3552, 3817, 4081
Cfr9I CCCGGG 3 cut(s) 3064, 3379, 3548
Csp6I GTAC 9 cut(s) 1339, 1693, 2139, 2982, 3201, 3537, 3655, 4051, 4186
CspCI CAANNNNNGTGG 2 cut(s) 1696, 1731
CviQI GTAC 9 cut(s) 1339, 1693, 2139, 2982, 3201, 3537, 3655, 4051, 4186
DriI GACNNNNNGTC 1 cut(s) 3165
EaeI YGGCCR 4 cut(s) 772, 1105, 1537, 4341
EagI CGGCCG 1 cut(s) 4341
Eam1104I CTCTTC 7 cut(s) 925, 1272, 1304, 2421, 2426, 3092, 3968
Eam1105I GACNNNNNGTC 1 cut(s) 3165
EarI CTCTTC 7 cut(s) 925, 1272, 1304, 2421, 2426, 3092, 3968
EciI GGCGGA 2 cut(s) 32, 1862
Ecl136II GAGCTC 2 cut(s) 1297, 1586
EclXI CGGCCG 1 cut(s) 4341
Eco130I CCWWGG 4 cut(s) 1254, 1342, 2204, 3793
Eco147I AGGCCT 1 cut(s) 1517
Eco24I GRGCYC 2 cut(s) 1299, 1588
Eco31I GGTCTC 1 cut(s) 3173
Eco32I GATATC 2 cut(s) 1054, 1066
Eco47I GGWCC 8 cut(s) 680, 1145, 1346, 1447, 1942, 3329, 3552, 3817
Eco47III AGCGCT 1 cut(s) 2751
Eco52I CGGCCG 1 cut(s) 4341
Eco53kI GAGCTC 2 cut(s) 1297, 1586
Eco57I CTGAAG 3 cut(s) 1504, 1798, 2538
Eco72I CACGTG 1 cut(s) 1381
Eco81I CCTNAGG 1 cut(s) 1946
Eco88I CYCGRG 7 cut(s) 391, 962, 1364, 2465, 3064, 3379, 3548
Eco91I GGTNACC 1 cut(s) 1929
EcoICRI GAGCTC 2 cut(s) 1297, 1586
EcoO109I RGGNCCY 2 cut(s) 680, 1942
EcoO65I GGTNACC 1 cut(s) 1929
EcoRI GAATTC 1 cut(s) 2270
EcoRII CCWGG 9 cut(s) 659, 685, 2166, 2796, 2954, 3176, 3513, 4208, 4270
EcoRV GATATC 2 cut(s) 1054, 1066
EcoT14I CCWWGG 4 cut(s) 1254, 1342, 2204, 3793
EcoT22I ATGCAT 3 cut(s) 976, 3630, 3898
EcoT38I GRGCYC 2 cut(s) 1299, 1588
ErhI CCWWGG 4 cut(s) 1254, 1342, 2204, 3793
Esp3I CGTCTC 1 cut(s) 854
FalI AAGNNNNNCTT 2 cut(s) 2682, 2714
FaqI GGGAC 3 cut(s) 2065, 3081, 3738
FauI CCCGC 1 cut(s) 1285
FblI GTMKAC 2 cut(s) 38, 313
FriOI GRGCYC 2 cut(s) 1299, 1588
FspBI CTAG 7 cut(s) 713, 900, 1011, 2025, 2127, 2352, 3071
GlaI GCGC 6 cut(s) 1118, 1281, 1679, 2293, 2295, 2751
GsaI CCCAGC 3 cut(s) 58, 281, 4369
GsuI CTGGAG 1 cut(s) 669
HaeII RGCGCY 1 cut(s) 2753
HhaI GCGC 6 cut(s) 1119, 1282, 1680, 2294, 2296, 2752
Hin1I GRCGYC 1 cut(s) 2793
Hin6I GCGC 6 cut(s) 1117, 1280, 1678, 2292, 2294, 2750
HinP1I GCGC 6 cut(s) 1117, 1280, 1678, 2292, 2294, 2750
HincII GTYRAC 1 cut(s) 39
HindII GTYRAC 1 cut(s) 39
HindIII AAGCTT 4 cut(s) 593, 728, 3353, 3609
HphI GGTGA 4 cut(s) 262, 1444, 3242, 4256
Hpy99I CGWCG 4 cut(s) 155, 196, 4349, 4364
HpyCH4IV ACGT 8 cut(s) 194, 267, 636, 863, 1380, 2793, 3304, 4359
HpySE526I ACGT 8 cut(s) 194, 267, 636, 863, 1380, 2793, 3304, 4359
Hsp92I GRCGYC 1 cut(s) 2793
HspAI GCGC 6 cut(s) 1117, 1280, 1678, 2292, 2294, 2750
KpnI GGTACC 1 cut(s) 2142
LguI GCTCTTC 1 cut(s) 1304
LweI GCATC 8 cut(s) 92, 207, 961, 2199, 2461, 3163, 3414, 4410
MaeI CTAG 7 cut(s) 713, 900, 1011, 2025, 2127, 2352, 3071
MaeII ACGT 8 cut(s) 194, 267, 636, 863, 1380, 2793, 3304, 4359
MbiI CCGCTC 1 cut(s) 73
MfeI CAATTG 2 cut(s) 1229, 2702
MflI RGATCY 5 cut(s) 618, 1950, 2305, 3559, 3748
MhlI GDGCHC 7 cut(s) 535, 1299, 1397, 1588, 1775, 2401, 3390
MlsI TGGCCA 3 cut(s) 774, 1107, 1539
MluNI TGGCCA 3 cut(s) 774, 1107, 1539
MlyI GAGTC 3 cut(s) 2668, 3448, 4069
MmeI TCCRAC 5 cut(s) 205, 846, 874, 2635, 3596
Mox20I TGGCCA 3 cut(s) 774, 1107, 1539
Mph1103I ATGCAT 3 cut(s) 976, 3630, 3898
MroXI GAANNNNTTC 3 cut(s) 2270, 3877, 4298
MscI TGGCCA 3 cut(s) 774, 1107, 1539
MslI CAYNNNNRTG 3 cut(s) 3113, 3146, 4103
Msp20I TGGCCA 3 cut(s) 774, 1107, 1539
MspA1I CMGCKG 2 cut(s) 1553, 2886
MunI CAATTG 2 cut(s) 1229, 2702
Mva1269I GAATGC 6 cut(s) 288, 974, 2051, 2257, 3811, 4258
MvaI CCWGG 9 cut(s) 661, 687, 2168, 2798, 2956, 3178, 3515, 4210, 4272
MvnI CGCG 4 cut(s) 1119, 1280, 2294, 3858
NcoI CCATGG 1 cut(s) 1254
NheI GCTAGC 2 cut(s) 2024, 2126
NsiI ATGCAT 3 cut(s) 976, 3630, 3898
NspI RCATGY 3 cut(s) 810, 1650, 2924
NspV TTCGAA 1 cut(s) 1374
PacI TTAATTAA 1 cut(s) 591
PaeR7I CTCGAG 1 cut(s) 1364
PagI TCATGA 2 cut(s) 978, 2262
PaqCI CACCTGC 1 cut(s) 217
PauI GCGCGC 1 cut(s) 2292
PceI AGGCCT 1 cut(s) 1517
PciI ACATGT 2 cut(s) 806, 2920
PciSI GCTCTTC 1 cut(s) 1304
PcsI WCGNNNNNNNCGW 1 cut(s) 1371
PctI GAATGC 6 cut(s) 288, 974, 2051, 2257, 3811, 4258
PdmI GAANNNNTTC 3 cut(s) 2270, 3877, 4298
PfeI GAWTC 9 cut(s) 383, 948, 1386, 1970, 2358, 2936, 3275, 3669, 3978
PflMI CCANNNNNTGG 1 cut(s) 913
PfoI TCCNGGA 2 cut(s) 96, 659
Ple19I CGATCG 1 cut(s) 2259
PleI GAGTC 3 cut(s) 2668, 3448, 4069
PmaCI CACGTG 1 cut(s) 1381
PmlI CACGTG 1 cut(s) 1381
PpsI GAGTC 3 cut(s) 2668, 3448, 4069
Ppu21I YACGTR 1 cut(s) 1381
PpuMI RGGWCCY 2 cut(s) 680, 1942
PscI ACATGT 2 cut(s) 806, 2920
PshAI GACNNNNGTC 1 cut(s) 3683
PshBI ATTAAT 2 cut(s) 587, 1824
Psp124BI GAGCTC 2 cut(s) 1299, 1588
Psp5II RGGWCCY 2 cut(s) 680, 1942
Psp6I CCWGG 9 cut(s) 659, 685, 2166, 2796, 2954, 3176, 3513, 4208, 4270
PspCI CACGTG 1 cut(s) 1381
PspEI GGTNACC 1 cut(s) 1929
PspFI CCCAGC 3 cut(s) 54, 277, 4365
PspGI CCWGG 9 cut(s) 659, 685, 2166, 2796, 2954, 3176, 3513, 4208, 4270
PspPI GGNCC 9 cut(s) 680, 1145, 1346, 1447, 1942, 3329, 3552, 3817, 4081
PspPPI RGGWCCY 2 cut(s) 680, 1942
PspXI VCTCGAGB 1 cut(s) 1364
PstI CTGCAG 2 cut(s) 1275, 3448
PstNI CAGNNNCTG 2 cut(s) 232, 4125
PsuI RGATCY 5 cut(s) 618, 1950, 2305, 3559, 3748
PteI GCGCGC 1 cut(s) 2292
PvuI CGATCG 1 cut(s) 2259
PvuII CAGCTG 1 cut(s) 1553
RsaI GTAC 9 cut(s) 1340, 1694, 2140, 2983, 3202, 3538, 3656, 4052, 4187
RsaNI GTAC 9 cut(s) 1339, 1693, 2139, 2982, 3201, 3537, 3655, 4051, 4186
RseI CAYNNNNRTG 3 cut(s) 3113, 3146, 4103
SacI GAGCTC 2 cut(s) 1299, 1588
SalI GTCGAC 1 cut(s) 37
SapI GCTCTTC 1 cut(s) 1304
Sau96I GGNCC 9 cut(s) 680, 1145, 1346, 1447, 1942, 3329, 3552, 3817, 4081
SchI GAGTC 3 cut(s) 2668, 3448, 4069
SduI GDGCHC 7 cut(s) 535, 1299, 1397, 1588, 1775, 2401, 3390
SfaNI GCATC 8 cut(s) 92, 207, 961, 2199, 2461, 3163, 3414, 4410
SfcI CTRYAG 4 cut(s) 1271, 1483, 3444, 3912
Sfr274I CTCGAG 1 cut(s) 1364
SfuI TTCGAA 1 cut(s) 1374
SinI GGWCC 8 cut(s) 680, 1145, 1346, 1447, 1942, 3329, 3552, 3817
SlaI CTCGAG 1 cut(s) 1364
SmaI CCCGGG 3 cut(s) 3066, 3381, 3550
SmiMI CAYNNNNRTG 3 cut(s) 3113, 3146, 4103
SmlI CTYRAG 8 cut(s) 317, 349, 925, 1034, 1364, 2198, 2345, 3428
SmoI CTYRAG 8 cut(s) 317, 349, 925, 1034, 1364, 2198, 2345, 3428
SpeI ACTAGT 1 cut(s) 1010
SseBI AGGCCT 1 cut(s) 1517
SspI AATATT 3 cut(s) 1460, 2742, 3365
SspMI CTAG 7 cut(s) 713, 900, 1011, 2025, 2127, 2352, 3071
SstI GAGCTC 2 cut(s) 1299, 1588
StuI AGGCCT 1 cut(s) 1517
StyI CCWWGG 4 cut(s) 1254, 1342, 2204, 3793
TaiI ACGT 8 cut(s) 197, 270, 639, 866, 1383, 2796, 3307, 4362
TaqII GACCGA 2 cut(s) 24, 3692
TatI WGTACW 4 cut(s) 1692, 2981, 3536, 3654
TauI GCSGC 7 cut(s) 8, 11, 145, 176, 3342, 4352, 4355
TfiI GAWTC 9 cut(s) 383, 948, 1386, 1970, 2358, 2936, 3275, 3669, 3978
TscAI CASTG 9 cut(s) 418, 540, 556, 858, 1747, 2725, 3442, 3608, 4077
TspGWI ACGGA 2 cut(s) 2951, 3109
TspMI CCCGGG 3 cut(s) 3064, 3379, 3548
TspRI CASTG 9 cut(s) 418, 540, 556, 858, 1747, 2725, 3442, 3608, 4077
Van91I CCANNNNNTGG 1 cut(s) 913
VpaK11BI GGWCC 8 cut(s) 680, 1145, 1346, 1447, 1942, 3329, 3552, 3817
VspI ATTAAT 2 cut(s) 587, 1824
XapI RAATTY 6 cut(s) 465, 493, 1793, 2270, 3046, 3998
XbaI TCTAGA 1 cut(s) 2351
XceI RCATGY 3 cut(s) 810, 1650, 2924
XhoI CTCGAG 1 cut(s) 1364
XmaI CCCGGG 3 cut(s) 3064, 3379, 3548
XmiI GTMKAC 2 cut(s) 38, 313
XmnI GAANNNNTTC 3 cut(s) 2270, 3877, 4298
XspI CTAG 7 cut(s) 713, 900, 1011, 2025, 2127, 2352, 3071
ZraI GACGTC 1 cut(s) 2794
Zsp2I ATGCAT 3 cut(s) 976, 3630, 3898
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.