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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1883 |
| AarI | CACCTGC | 1 cut(s) | 983 |
| AatII | GACGTC | 1 cut(s) | 87 |
| Acc36I | ACCTGC | 1 cut(s) | 983 |
| Acc65I | GGTACC | 1 cut(s) | 1019 |
| AccB1I | GGYRCC | 5 cut(s) | 19, 971, 1019, 1291, 1792 |
| AccI | GTMKAC | 2 cut(s) | 51, 641 |
| AccII | CGCG | 2 cut(s) | 8, 10 |
| AciI | CCGC | 3 cut(s) | 10, 1169, 1966 |
| AclWI | GGATC | 2 cut(s) | 86, 1532 |
| AcoI | YGGCCR | 1 cut(s) | 1453 |
| AcsI | RAATTY | 3 cut(s) | 72, 549, 1493 |
| AcuI | CTGAAG | 1 cut(s) | 432 |
| AcvI | CACGTG | 2 cut(s) | 704, 1183 |
| AcyI | GRCGYC | 1 cut(s) | 84 |
| AfaI | GTAC | 4 cut(s) | 459, 1021, 1316, 1821 |
| AfeI | AGCGCT | 1 cut(s) | 1301 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 200, 1141, 1142, 1729 |
| AgeI | ACCGGT | 1 cut(s) | 1219 |
| AhlI | ACTAGT | 1 cut(s) | 1346 |
| AjiI | CACGTC | 1 cut(s) | 1036 |
| AjnI | CCWGG | 1 cut(s) | 87 |
| AluBI | AGCT | 9 cut(s) | 39, 544, 655, 727, 778, 1064, 1279, 1613, 1979 |
| AluI | AGCT | 9 cut(s) | 39, 544, 655, 727, 778, 1064, 1279, 1613, 1979 |
| Alw21I | GWGCWC | 1 cut(s) | 1066 |
| Alw26I | GTCTC | 3 cut(s) | 148, 728, 1503 |
| AlwI | GGATC | 2 cut(s) | 86, 1532 |
| Aor51HI | AGCGCT | 1 cut(s) | 1301 |
| AoxI | GGCC | 7 cut(s) | 41, 105, 407, 450, 890, 1190, 1453 |
| ApeKI | GCWGC | 3 cut(s) | 190, 1469, 1544 |
| ApoI | RAATTY | 3 cut(s) | 72, 549, 1493 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1367, 1399 |
| AseI | ATTAAT | 1 cut(s) | 897 |
| AsiGI | ACCGGT | 1 cut(s) | 1219 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1158 |
| Asp718I | GGTACC | 1 cut(s) | 1019 |
| AspLEI | GCGC | 2 cut(s) | 10, 1302 |
| AspS9I | GGNCC | 5 cut(s) | 42, 106, 451, 1696, 1963 |
| AsuC2I | CCSGG | 3 cut(s) | 1644, 1815, 1833 |
| AsuHPI | GGTGA | 7 cut(s) | 68, 551, 685, 1115, 1157, 1234, 1600 |
| AsuII | TTCGAA | 1 cut(s) | 1210 |
| AvaII | GGWCC | 2 cut(s) | 1696, 1963 |
| AxyI | CCTNAGG | 1 cut(s) | 1194 |
| BaeGI | GKGCMC | 2 cut(s) | 24, 1296 |
| BalI | TGGCCA | 1 cut(s) | 1455 |
| BanI | GGYRCC | 5 cut(s) | 19, 971, 1019, 1291, 1792 |
| BanII | GRGCYC | 1 cut(s) | 1066 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 329, 361 |
| BbrPI | CACGTG | 2 cut(s) | 704, 1183 |
| BbsI | GAAGAC | 2 cut(s) | 1219, 1931 |
| Bbv12I | GWGCWC | 1 cut(s) | 1066 |
| BbvI | GCAGC | 3 cut(s) | 177, 1481, 1531 |
| BccI | CCATC | 5 cut(s) | 357, 514, 1004, 1094, 1144 |
| BciT130I | CCWGG | 1 cut(s) | 89 |
| BciVI | GTATCC | 1 cut(s) | 85 |
| BclI | TGATCA | 1 cut(s) | 1888 |
| BcnI | CCSGG | 3 cut(s) | 1644, 1815, 1833 |
| BcoDI | GTCTC | 3 cut(s) | 148, 728, 1503 |
| BcuI | ACTAGT | 1 cut(s) | 1346 |
| BfaI | CTAG | 4 cut(s) | 383, 545, 584, 1347 |
| BfmI | CTRYAG | 3 cut(s) | 288, 957, 2000 |
| BfoI | RGCGCY | 1 cut(s) | 1303 |
| BfuAI | ACCTGC | 1 cut(s) | 983 |
| BfuI | GTATCC | 1 cut(s) | 85 |
| BisI | GCNGC | 3 cut(s) | 191, 1470, 1545 |
| BlsI | GCNGC | 3 cut(s) | 192, 1471, 1546 |
| Bme1390I | CCNGG | 4 cut(s) | 89, 1644, 1815, 1833 |
| Bme18I | GGWCC | 2 cut(s) | 1696, 1963 |
| BmgBI | CACGTC | 1 cut(s) | 1036 |
| BmgT120I | GGNCC | 5 cut(s) | 42, 106, 451, 1696, 1963 |
| BmiI | GGNNCC | 7 cut(s) | 21, 108, 973, 1021, 1293, 1698, 1794 |
| BmrFI | CCNGG | 4 cut(s) | 89, 1644, 1815, 1833 |
| BmsI | GCATC | 1 cut(s) | 1108 |
| BpiI | GAAGAC | 2 cut(s) | 1219, 1931 |
| BpmI | CTGGAG | 1 cut(s) | 749 |
| Bpu14I | TTCGAA | 1 cut(s) | 1210 |
| BpuEI | CTTGAG | 2 cut(s) | 561, 1929 |
| BpuMI | CCSGG | 3 cut(s) | 1644, 1815, 1833 |
| BsaAI | YACGTR | 2 cut(s) | 704, 1183 |
| BsaHI | GRCGYC | 1 cut(s) | 84 |
| BsaJI | CCNNGG | 3 cut(s) | 260, 1456, 1643 |
| BsaWI | WCCGGW | 1 cut(s) | 1219 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 615, 645 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 200, 1141, 1142, 1729 |
| Bse118I | RCCGGY | 1 cut(s) | 1219 |
| Bse1I | ACTGG | 1 cut(s) | 702 |
| Bse21I | CCTNAGG | 1 cut(s) | 1194 |
| BseBI | CCWGG | 1 cut(s) | 89 |
| BseDI | CCNNGG | 3 cut(s) | 260, 1456, 1643 |
| BseGI | GGATG | 3 cut(s) | 1105, 1378, 1684 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 200, 1141, 1142, 1729 |
| BseMII | CTCAG | 2 cut(s) | 1185, 1407 |
| BseNI | ACTGG | 1 cut(s) | 702 |
| BseRI | GAGGAG | 3 cut(s) | 401, 746, 1211 |
| BseSI | GKGCMC | 2 cut(s) | 24, 1296 |
| BseXI | GCAGC | 3 cut(s) | 177, 1481, 1531 |
| Bsh1236I | CGCG | 2 cut(s) | 8, 10 |
| BshFI | GGCC | 7 cut(s) | 43, 107, 409, 452, 892, 1192, 1455 |
| BshNI | GGYRCC | 5 cut(s) | 19, 971, 1019, 1291, 1792 |
| BshTI | ACCGGT | 1 cut(s) | 1219 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1066 |
| BsiSI | CCGG | 4 cut(s) | 1220, 1643, 1814, 1832 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 200, 1141, 1142, 1729 |
| BsmAI | GTCTC | 3 cut(s) | 148, 728, 1503 |
| BsmBI | CGTCTC | 1 cut(s) | 1503 |
| BsmI | GAATGC | 1 cut(s) | 242 |
| BsnI | GGCC | 7 cut(s) | 43, 107, 409, 452, 892, 1192, 1455 |
| Bsp119I | TTCGAA | 1 cut(s) | 1210 |
| Bsp1286I | GDGCHC | 3 cut(s) | 24, 1066, 1296 |
| Bsp1407I | TGTACA | 1 cut(s) | 1314 |
| Bsp143I | GATC | 7 cut(s) | 78, 718, 1429, 1537, 1565, 1615, 1888 |
| Bsp19I | CCATGG | 1 cut(s) | 260 |
| BspACI | CCGC | 3 cut(s) | 10, 1169, 1966 |
| BspANI | GGCC | 7 cut(s) | 43, 107, 409, 452, 892, 1192, 1455 |
| BspCNI | CTCAG | 2 cut(s) | 1186, 1408 |
| BspFNI | CGCG | 2 cut(s) | 8, 10 |
| BspHI | TCATGA | 1 cut(s) | 1335 |
| BspLI | GGNNCC | 7 cut(s) | 21, 108, 973, 1021, 1293, 1698, 1794 |
| BspMAI | CTGCAG | 1 cut(s) | 961 |
| BspMI | ACCTGC | 1 cut(s) | 983 |
| BspPI | GGATC | 2 cut(s) | 86, 1532 |
| BspQI | GCTCTTC | 1 cut(s) | 768 |
| BspT104I | TTCGAA | 1 cut(s) | 1210 |
| BspT107I | GGYRCC | 5 cut(s) | 19, 971, 1019, 1291, 1792 |
| BsrFI | RCCGGY | 1 cut(s) | 1219 |
| BsrGI | TGTACA | 1 cut(s) | 1314 |
| BsrI | ACTGG | 1 cut(s) | 702 |
| BssAI | RCCGGY | 1 cut(s) | 1219 |
| BssECI | CCNNGG | 3 cut(s) | 260, 1456, 1643 |
| BssMI | GATC | 7 cut(s) | 78, 718, 1429, 1537, 1565, 1615, 1888 |
| BssNI | GRCGYC | 1 cut(s) | 84 |
| BssT1I | CCWWGG | 2 cut(s) | 260, 1456 |
| Bst2UI | CCWGG | 1 cut(s) | 89 |
| Bst4CI | ACNGT | 4 cut(s) | 56, 431, 1024, 1366 |
| Bst6I | CTCTTC | 3 cut(s) | 332, 768, 1409 |
| BstACI | GRCGYC | 1 cut(s) | 84 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1996 |
| BstAUI | TGTACA | 1 cut(s) | 1314 |
| BstBAI | YACGTR | 2 cut(s) | 704, 1183 |
| BstBI | TTCGAA | 1 cut(s) | 1210 |
| BstC8I | GCNNGC | 3 cut(s) | 6, 41, 142 |
| BstDEI | CTNAG | 4 cut(s) | 684, 1194, 1416, 1435 |
| BstDSI | CCRYGG | 1 cut(s) | 260 |
| BstF5I | GGATG | 3 cut(s) | 1105, 1378, 1684 |
| BstFNI | CGCG | 2 cut(s) | 8, 10 |
| BstH2I | RGCGCY | 1 cut(s) | 1303 |
| BstHHI | GCGC | 2 cut(s) | 10, 1302 |
| BstKTI | GATC | 7 cut(s) | 81, 721, 1432, 1540, 1568, 1618, 1891 |
| BstMAI | GTCTC | 3 cut(s) | 148, 728, 1503 |
| BstMBI | GATC | 7 cut(s) | 78, 718, 1429, 1537, 1565, 1615, 1888 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 232, 661, 1996 |
| BstNI | CCWGG | 1 cut(s) | 89 |
| BstNSI | RCATGY | 1 cut(s) | 1325 |
| BstSCI | CCNGG | 4 cut(s) | 87, 1642, 1813, 1831 |
| BstSFI | CTRYAG | 3 cut(s) | 288, 957, 2000 |
| BstSLI | GKGCMC | 2 cut(s) | 24, 1296 |
| BstUI | CGCG | 2 cut(s) | 8, 10 |
| BstV1I | GCAGC | 3 cut(s) | 177, 1481, 1531 |
| BstV2I | GAAGAC | 2 cut(s) | 1219, 1931 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1369 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1194 |
| BsuI | GTATCC | 1 cut(s) | 85 |
| BsuRI | GGCC | 7 cut(s) | 43, 107, 409, 452, 892, 1192, 1455 |
| BtgI | CCRYGG | 1 cut(s) | 260 |
| BtgZI | GCGATG | 1 cut(s) | 1131 |
| BtrI | CACGTC | 1 cut(s) | 1036 |
| BtsCI | GGATG | 3 cut(s) | 1105, 1378, 1684 |
| BtsI | GCAGTG | 2 cut(s) | 993, 1926 |
| BtsIMutI | CAGTG | 3 cut(s) | 993, 1362, 1926 |
| BveI | ACCTGC | 1 cut(s) | 983 |
| Cac8I | GCNNGC | 3 cut(s) | 6, 41, 142 |
| CciI | TCATGA | 1 cut(s) | 1335 |
| CfoI | GCGC | 2 cut(s) | 10, 1302 |
| Cfr10I | RCCGGY | 1 cut(s) | 1219 |
| Cfr13I | GGNCC | 5 cut(s) | 42, 106, 451, 1696, 1963 |
| Csp6I | GTAC | 4 cut(s) | 458, 1020, 1315, 1820 |
| CspAI | ACCGGT | 1 cut(s) | 1219 |
| CviQI | GTAC | 4 cut(s) | 458, 1020, 1315, 1820 |
| DdeI | CTNAG | 4 cut(s) | 684, 1194, 1416, 1435 |
| DpnI | GATC | 7 cut(s) | 80, 720, 1431, 1539, 1567, 1617, 1890 |
| DpnII | GATC | 7 cut(s) | 78, 718, 1429, 1537, 1565, 1615, 1888 |
| EaeI | YGGCCR | 1 cut(s) | 1453 |
| Eam1104I | CTCTTC | 3 cut(s) | 332, 768, 1409 |
| EarI | CTCTTC | 3 cut(s) | 332, 768, 1409 |
| Ecl136II | GAGCTC | 1 cut(s) | 1064 |
| Eco130I | CCWWGG | 2 cut(s) | 260, 1456 |
| Eco24I | GRGCYC | 1 cut(s) | 1066 |
| Eco47I | GGWCC | 2 cut(s) | 1696, 1963 |
| Eco47III | AGCGCT | 1 cut(s) | 1301 |
| Eco53kI | GAGCTC | 1 cut(s) | 1064 |
| Eco57I | CTGAAG | 1 cut(s) | 432 |
| Eco72I | CACGTG | 2 cut(s) | 704, 1183 |
| Eco81I | CCTNAGG | 1 cut(s) | 1194 |
| EcoICRI | GAGCTC | 1 cut(s) | 1064 |
| EcoRII | CCWGG | 1 cut(s) | 87 |
| EcoT14I | CCWWGG | 2 cut(s) | 260, 1456 |
| EcoT22I | ATGCAT | 1 cut(s) | 244 |
| EcoT38I | GRGCYC | 1 cut(s) | 1066 |
| ErhI | CCWWGG | 2 cut(s) | 260, 1456 |
| Esp3I | CGTCTC | 1 cut(s) | 1503 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 155, 187, 1263, 1295, 1938, 1970 |
| FauNDI | CATATG | 1 cut(s) | 1670 |
| FbaI | TGATCA | 1 cut(s) | 1888 |
| FblI | GTMKAC | 2 cut(s) | 51, 641 |
| Fnu4HI | GCNGC | 3 cut(s) | 191, 1470, 1545 |
| FokI | GGATG | 3 cut(s) | 1112, 1385, 1691 |
| FriOI | GRGCYC | 1 cut(s) | 1066 |
| Fsp4HI | GCNGC | 3 cut(s) | 191, 1470, 1545 |
| FspBI | CTAG | 4 cut(s) | 383, 545, 584, 1347 |
| GlaI | GCGC | 2 cut(s) | 9, 1301 |
| GluI | GCNGC | 3 cut(s) | 191, 1470, 1545 |
| GsuI | CTGGAG | 1 cut(s) | 749 |
| HaeII | RGCGCY | 1 cut(s) | 1303 |
| HaeIII | GGCC | 7 cut(s) | 43, 107, 409, 452, 892, 1192, 1455 |
| HapII | CCGG | 4 cut(s) | 1220, 1643, 1814, 1832 |
| HhaI | GCGC | 2 cut(s) | 10, 1302 |
| Hin1I | GRCGYC | 1 cut(s) | 84 |
| Hin6I | GCGC | 2 cut(s) | 8, 1300 |
| HinP1I | GCGC | 2 cut(s) | 8, 1300 |
| HincII | GTYRAC | 2 cut(s) | 132, 910 |
| HindII | GTYRAC | 2 cut(s) | 132, 910 |
| HindIII | AAGCTT | 1 cut(s) | 653 |
| HinfI | GANTC | 4 cut(s) | 330, 1108, 1591, 1927 |
| HpaII | CCGG | 4 cut(s) | 1220, 1643, 1814, 1832 |
| HphI | GGTGA | 7 cut(s) | 68, 551, 685, 1115, 1157, 1234, 1600 |
| Hpy166II | GTNNAC | 7 cut(s) | 52, 132, 326, 458, 642, 910, 1820 |
| Hpy188I | TCNGA | 5 cut(s) | 282, 856, 1570, 1908, 1932 |
| Hpy8I | GTNNAC | 7 cut(s) | 52, 132, 326, 458, 642, 910, 1820 |
| Hpy99I | CGWCG | 1 cut(s) | 86 |
| HpyAV | CCTTC | 6 cut(s) | 259, 407, 442, 529, 1066, 1867 |
| HpyCH4III | ACNGT | 4 cut(s) | 56, 431, 1024, 1366 |
| HpyCH4IV | ACGT | 4 cut(s) | 84, 703, 1035, 1182 |
| HpyCH4V | TGCA | 9 cut(s) | 242, 434, 500, 919, 959, 1121, 1483, 1751, 1990 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 232, 661, 1996 |
| HpyF3I | CTNAG | 4 cut(s) | 684, 1194, 1416, 1435 |
| HpySE526I | ACGT | 4 cut(s) | 84, 703, 1035, 1182 |
| Hsp92I | GRCGYC | 1 cut(s) | 84 |
| HspAI | GCGC | 2 cut(s) | 8, 1300 |
| KpnI | GGTACC | 1 cut(s) | 1023 |
| Ksp22I | TGATCA | 1 cut(s) | 1888 |
| Kzo9I | GATC | 7 cut(s) | 78, 718, 1429, 1537, 1565, 1615, 1888 |
| LguI | GCTCTTC | 1 cut(s) | 768 |
| LmnI | GCTCC | 3 cut(s) | 724, 1061, 1069 |
| Lsp1109I | GCAGC | 3 cut(s) | 177, 1481, 1531 |
| LweI | GCATC | 1 cut(s) | 1108 |
| MaeI | CTAG | 4 cut(s) | 383, 545, 584, 1347 |
| MaeII | ACGT | 4 cut(s) | 84, 703, 1035, 1182 |
| MaeIII | GTNAC | 2 cut(s) | 487, 1178 |
| MalI | GATC | 7 cut(s) | 80, 720, 1431, 1539, 1567, 1617, 1890 |
| MboI | GATC | 7 cut(s) | 78, 718, 1429, 1537, 1565, 1615, 1888 |
| MhlI | GDGCHC | 3 cut(s) | 24, 1066, 1296 |
| MlsI | TGGCCA | 1 cut(s) | 1455 |
| MluNI | TGGCCA | 1 cut(s) | 1455 |
| MlyI | GAGTC | 2 cut(s) | 339, 1921 |
| MmeI | TCCRAC | 5 cut(s) | 601, 1153, 1350, 1497, 1931 |
| Mox20I | TGGCCA | 1 cut(s) | 1455 |
| Mph1103I | ATGCAT | 1 cut(s) | 244 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1158 |
| MscI | TGGCCA | 1 cut(s) | 1455 |
| MseI | TTAA | 8 cut(s) | 27, 173, 462, 651, 782, 897, 1044, 1755 |
| Msp20I | TGGCCA | 1 cut(s) | 1455 |
| MspI | CCGG | 4 cut(s) | 1220, 1643, 1814, 1832 |
| MspR9I | CCNGG | 4 cut(s) | 89, 1644, 1815, 1833 |
| Mva1269I | GAATGC | 1 cut(s) | 242 |
| MvaI | CCWGG | 1 cut(s) | 89 |
| MvnI | CGCG | 2 cut(s) | 8, 10 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 232, 661, 1996 |
| NciI | CCSGG | 3 cut(s) | 1644, 1815, 1833 |
| NcoI | CCATGG | 1 cut(s) | 260 |
| NdeI | CATATG | 1 cut(s) | 1670 |
| NdeII | GATC | 7 cut(s) | 78, 718, 1429, 1537, 1565, 1615, 1888 |
| NlaIV | GGNNCC | 7 cut(s) | 21, 108, 973, 1021, 1293, 1698, 1794 |
| NmuCI | GTSAC | 2 cut(s) | 487, 1178 |
| NsiI | ATGCAT | 1 cut(s) | 244 |
| NspI | RCATGY | 1 cut(s) | 1325 |
| NspV | TTCGAA | 1 cut(s) | 1210 |
| PagI | TCATGA | 1 cut(s) | 1335 |
| PaqCI | CACCTGC | 1 cut(s) | 983 |
| PciSI | GCTCTTC | 1 cut(s) | 768 |
| PctI | GAATGC | 1 cut(s) | 242 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1158 |
| PfeI | GAWTC | 2 cut(s) | 1108, 1591 |
| PflFI | GACNNNGTC | 1 cut(s) | 1961 |
| PfoI | TCCNGGA | 1 cut(s) | 87 |
| PinAI | ACCGGT | 1 cut(s) | 1219 |
| PkrI | GCNGC | 3 cut(s) | 192, 1471, 1546 |
| PleI | GAGTC | 2 cut(s) | 338, 1921 |
| PmaCI | CACGTG | 2 cut(s) | 704, 1183 |
| PmlI | CACGTG | 2 cut(s) | 704, 1183 |
| PpsI | GAGTC | 2 cut(s) | 338, 1921 |
| Ppu21I | YACGTR | 2 cut(s) | 704, 1183 |
| PshBI | ATTAAT | 1 cut(s) | 897 |
| PsiI | TTATAA | 1 cut(s) | 1883 |
| Psp124BI | GAGCTC | 1 cut(s) | 1066 |
| Psp6I | CCWGG | 1 cut(s) | 87 |
| PspCI | CACGTG | 2 cut(s) | 704, 1183 |
| PspGI | CCWGG | 1 cut(s) | 87 |
| PspN4I | GGNNCC | 7 cut(s) | 21, 108, 973, 1021, 1293, 1698, 1794 |
| PspPI | GGNCC | 5 cut(s) | 42, 106, 451, 1696, 1963 |
| PstI | CTGCAG | 1 cut(s) | 961 |
| PsyI | GACNNNGTC | 1 cut(s) | 1961 |
| RsaI | GTAC | 4 cut(s) | 459, 1021, 1316, 1821 |
| RsaNI | GTAC | 4 cut(s) | 458, 1020, 1315, 1820 |
| SacI | GAGCTC | 1 cut(s) | 1066 |
| SapI | GCTCTTC | 1 cut(s) | 768 |
| SaqAI | TTAA | 8 cut(s) | 27, 173, 462, 651, 782, 897, 1044, 1755 |
| SatI | GCNGC | 3 cut(s) | 191, 1470, 1545 |
| Sau3AI | GATC | 7 cut(s) | 78, 718, 1429, 1537, 1565, 1615, 1888 |
| Sau96I | GGNCC | 5 cut(s) | 42, 106, 451, 1696, 1963 |
| SchI | GAGTC | 2 cut(s) | 339, 1921 |
| ScrFI | CCNGG | 4 cut(s) | 89, 1644, 1815, 1833 |
| SduI | GDGCHC | 3 cut(s) | 24, 1066, 1296 |
| SfaNI | GCATC | 1 cut(s) | 1108 |
| SfcI | CTRYAG | 3 cut(s) | 288, 957, 2000 |
| SfuI | TTCGAA | 1 cut(s) | 1210 |
| SinI | GGWCC | 2 cut(s) | 1696, 1963 |
| SmlI | CTYRAG | 2 cut(s) | 576, 1944 |
| SmoI | CTYRAG | 2 cut(s) | 576, 1944 |
| SpeI | ACTAGT | 1 cut(s) | 1346 |
| SsiI | CCGC | 3 cut(s) | 10, 1169, 1966 |
| SspMI | CTAG | 4 cut(s) | 383, 545, 584, 1347 |
| SstI | GAGCTC | 1 cut(s) | 1066 |
| StyD4I | CCNGG | 4 cut(s) | 87, 1642, 1813, 1831 |
| StyI | CCWWGG | 2 cut(s) | 260, 1456 |
| TaaI | ACNGT | 4 cut(s) | 56, 431, 1024, 1366 |
| TaiI | ACGT | 4 cut(s) | 87, 706, 1038, 1185 |
| TaqI | TCGA | 4 cut(s) | 81, 1162, 1210, 1266 |
| TatI | WGTACW | 2 cut(s) | 457, 1314 |
| TfiI | GAWTC | 2 cut(s) | 1108, 1591 |
| Tru1I | TTAA | 8 cut(s) | 27, 173, 462, 651, 782, 897, 1044, 1755 |
| Tru9I | TTAA | 8 cut(s) | 27, 173, 462, 651, 782, 897, 1044, 1755 |
| TscAI | CASTG | 3 cut(s) | 993, 1369, 1926 |
| TseFI | GTSAC | 2 cut(s) | 487, 1178 |
| TseI | GCWGC | 3 cut(s) | 190, 1469, 1544 |
| Tsp45I | GTSAC | 2 cut(s) | 487, 1178 |
| TspGWI | ACGGA | 1 cut(s) | 627 |
| TspRI | CASTG | 3 cut(s) | 993, 1369, 1926 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1961 |
| VpaK11BI | GGWCC | 2 cut(s) | 1696, 1963 |
| VspI | ATTAAT | 1 cut(s) | 897 |
| XapI | RAATTY | 3 cut(s) | 72, 549, 1493 |
| XceI | RCATGY | 1 cut(s) | 1325 |
| XmiI | GTMKAC | 2 cut(s) | 51, 641 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1158 |
| XspI | CTAG | 4 cut(s) | 383, 545, 584, 1347 |
| ZraI | GACGTC | 1 cut(s) | 85 |
| Zsp2I | ATGCAT | 1 cut(s) | 244 |