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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 1520 |
| Acc16I | TGCGCA | 1 cut(s) | 2138 |
| Acc65I | GGTACC | 1 cut(s) | 1163 |
| AccB1I | GGYRCC | 2 cut(s) | 130, 1163 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 970 |
| AccI | GTMKAC | 1 cut(s) | 1857 |
| AccII | CGCG | 1 cut(s) | 121 |
| AciI | CCGC | 5 cut(s) | 121, 1922, 2007, 2035, 2090 |
| AclWI | GGATC | 3 cut(s) | 85, 1673, 1740 |
| AcoI | YGGCCR | 3 cut(s) | 1306, 1594, 2148 |
| AcsI | RAATTY | 1 cut(s) | 984 |
| AcuI | CTGAAG | 2 cut(s) | 543, 1281 |
| AcvI | CACGTG | 1 cut(s) | 815 |
| AfaI | GTAC | 5 cut(s) | 5, 1165, 1434, 1992, 2039 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 310, 311, 408, 970, 997, 1285, 1894 |
| AflIII | ACRYGT | 1 cut(s) | 1118 |
| AgsI | TTSAA | 8 cut(s) | 175, 211, 274, 707, 958, 1216, 1256, 1741 |
| AhlI | ACTAGT | 1 cut(s) | 1487 |
| Alw21I | GWGCWC | 3 cut(s) | 303, 891, 1545 |
| Alw26I | GTCTC | 2 cut(s) | 839, 969 |
| AlwI | GGATC | 3 cut(s) | 85, 1673, 1740 |
| Ama87I | CYCGRG | 2 cut(s) | 712, 1984 |
| AoxI | GGCC | 6 cut(s) | 152, 1248, 1306, 1334, 1594, 2148 |
| ApeKI | GCWGC | 4 cut(s) | 367, 908, 1610, 1685 |
| ApoI | RAATTY | 1 cut(s) | 984 |
| AseI | ATTAAT | 1 cut(s) | 2055 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 441, 1071 |
| Asp718I | GGTACC | 1 cut(s) | 1163 |
| AspLEI | GCGC | 3 cut(s) | 121, 1624, 2139 |
| AspS9I | GGNCC | 4 cut(s) | 153, 1248, 1718, 1885 |
| AsuC2I | CCSGG | 5 cut(s) | 1785, 1883, 1985, 1986, 2004 |
| AsuHPI | GGTGA | 6 cut(s) | 179, 622, 662, 733, 1072, 1301 |
| AsuII | TTCGAA | 1 cut(s) | 1354 |
| AvaI | CYCGRG | 2 cut(s) | 712, 1984 |
| AvaII | GGWCC | 2 cut(s) | 1718, 1885 |
| AxyI | CCTNAGG | 1 cut(s) | 1338 |
| BaeGI | GKGCMC | 1 cut(s) | 135 |
| BalI | TGGCCA | 3 cut(s) | 1308, 1596, 2150 |
| BanI | GGYRCC | 2 cut(s) | 130, 1163 |
| BanII | GRGCYC | 2 cut(s) | 891, 1545 |
| BbrPI | CACGTG | 1 cut(s) | 815 |
| BbsI | GAAGAC | 2 cut(s) | 941, 1516 |
| Bbv12I | GWGCWC | 3 cut(s) | 303, 891, 1545 |
| BbvI | GCAGC | 4 cut(s) | 379, 895, 1622, 1697 |
| BccI | CCATC | 6 cut(s) | 143, 572, 1148, 1238, 1288, 1378 |
| BceAI | ACGGC | 1 cut(s) | 1911 |
| BciVI | GTATCC | 3 cut(s) | 196, 1022, 1129 |
| BclI | TGATCA | 1 cut(s) | 1408 |
| BcnI | CCSGG | 5 cut(s) | 1785, 1883, 1985, 1986, 2004 |
| BcoDI | GTCTC | 2 cut(s) | 839, 969 |
| BcuI | ACTAGT | 1 cut(s) | 1487 |
| BfaI | CTAG | 6 cut(s) | 413, 494, 600, 809, 1280, 1488 |
| BfmI | CTRYAG | 4 cut(s) | 95, 979, 1683, 1908 |
| BfuI | GTATCC | 3 cut(s) | 196, 1022, 1129 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 376 |
| BisI | GCNGC | 4 cut(s) | 368, 909, 1611, 1686 |
| BlpI | GCTNAGC | 1 cut(s) | 1416 |
| BlsI | GCNGC | 4 cut(s) | 369, 910, 1612, 1687 |
| Bme1390I | CCNGG | 5 cut(s) | 1785, 1883, 1985, 1986, 2004 |
| Bme18I | GGWCC | 2 cut(s) | 1718, 1885 |
| BmeT110I | CYCGRG | 2 cut(s) | 712, 1984 |
| BmgT120I | GGNCC | 4 cut(s) | 153, 1248, 1718, 1885 |
| BmiI | GGNNCC | 3 cut(s) | 132, 1165, 1249 |
| BmrFI | CCNGG | 5 cut(s) | 1785, 1883, 1985, 1986, 2004 |
| BmsI | GCATC | 1 cut(s) | 1800 |
| BpiI | GAAGAC | 2 cut(s) | 941, 1516 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1409, 1441 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1416 |
| Bpu14I | TTCGAA | 1 cut(s) | 1354 |
| BpuEI | CTTGAG | 2 cut(s) | 672, 677 |
| BpuMI | CCSGG | 5 cut(s) | 1785, 1883, 1985, 1986, 2004 |
| Bsa29I | ATCGAT | 1 cut(s) | 466 |
| BsaAI | YACGTR | 1 cut(s) | 815 |
| BsaJI | CCNNGG | 5 cut(s) | 371, 795, 1056, 1597, 1984 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 310, 311, 408, 970, 997, 1285, 1894 |
| Bse1I | ACTGG | 1 cut(s) | 240 |
| Bse21I | CCTNAGG | 1 cut(s) | 1338 |
| BseCI | ATCGAT | 1 cut(s) | 466 |
| BseDI | CCNNGG | 5 cut(s) | 371, 795, 1056, 1597, 1984 |
| BseGI | GGATG | 5 cut(s) | 1183, 1189, 1200, 1249, 1855 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 310, 311, 408, 970, 997, 1285, 1894 |
| BseMII | CTCAG | 4 cut(s) | 1329, 1407, 1548, 1635 |
| BseNI | ACTGG | 1 cut(s) | 240 |
| BseRI | GAGGAG | 3 cut(s) | 515, 857, 1355 |
| BseSI | GKGCMC | 1 cut(s) | 135 |
| BseXI | GCAGC | 4 cut(s) | 379, 895, 1622, 1697 |
| BsgI | GTGCAG | 1 cut(s) | 849 |
| Bsh1236I | CGCG | 1 cut(s) | 121 |
| BshFI | GGCC | 6 cut(s) | 154, 1250, 1308, 1336, 1596, 2150 |
| BshNI | GGYRCC | 2 cut(s) | 130, 1163 |
| BshVI | ATCGAT | 1 cut(s) | 466 |
| BsiHKAI | GWGCWC | 3 cut(s) | 303, 891, 1545 |
| BsiHKCI | CYCGRG | 2 cut(s) | 712, 1984 |
| BsiSI | CCGG | 4 cut(s) | 1784, 1883, 1985, 2003 |
| BslFI | GGGAC | 1 cut(s) | 596 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 310, 311, 408, 970, 997, 1285, 1894 |
| BsmAI | GTCTC | 2 cut(s) | 839, 969 |
| BsmFI | GGGAC | 1 cut(s) | 596 |
| BsmI | GAATGC | 2 cut(s) | 269, 1924 |
| BsnI | GGCC | 6 cut(s) | 154, 1250, 1308, 1336, 1596, 2150 |
| BsoBI | CYCGRG | 2 cut(s) | 712, 1984 |
| Bsp119I | TTCGAA | 1 cut(s) | 1354 |
| Bsp1286I | GDGCHC | 4 cut(s) | 135, 303, 891, 1545 |
| Bsp1407I | TGTACA | 1 cut(s) | 3 |
| Bsp143I | GATC | 7 cut(s) | 77, 1408, 1570, 1678, 1706, 1732, 1756 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1416 |
| Bsp19I | CCATGG | 1 cut(s) | 371 |
| BspACI | CCGC | 5 cut(s) | 121, 1922, 2007, 2035, 2090 |
| BspANI | GGCC | 6 cut(s) | 154, 1250, 1308, 1336, 1596, 2150 |
| BspCNI | CTCAG | 4 cut(s) | 1330, 1408, 1549, 1636 |
| BspDI | ATCGAT | 1 cut(s) | 466 |
| BspFNI | CGCG | 1 cut(s) | 121 |
| BspLI | GGNNCC | 3 cut(s) | 132, 1165, 1249 |
| BspMAI | CTGCAG | 2 cut(s) | 1687, 1912 |
| BspPI | GGATC | 3 cut(s) | 85, 1673, 1740 |
| BspQI | GCTCTTC | 2 cut(s) | 879, 1550 |
| BspT104I | TTCGAA | 1 cut(s) | 1354 |
| BspT107I | GGYRCC | 2 cut(s) | 130, 1163 |
| BsrGI | TGTACA | 1 cut(s) | 3 |
| BsrI | ACTGG | 1 cut(s) | 240 |
| BssECI | CCNNGG | 5 cut(s) | 371, 795, 1056, 1597, 1984 |
| BssMI | GATC | 7 cut(s) | 77, 1408, 1570, 1678, 1706, 1732, 1756 |
| BssT1I | CCWWGG | 4 cut(s) | 371, 795, 1056, 1597 |
| Bst4CI | ACNGT | 5 cut(s) | 99, 167, 980, 1168, 1507 |
| Bst6I | CTCTTC | 6 cut(s) | 443, 492, 879, 1062, 1351, 1550 |
| BstAUI | TGTACA | 1 cut(s) | 3 |
| BstBAI | YACGTR | 1 cut(s) | 815 |
| BstBI | TTCGAA | 1 cut(s) | 1354 |
| BstC8I | GCNNGC | 3 cut(s) | 152, 253, 873 |
| BstDEI | CTNAG | 7 cut(s) | 126, 1338, 1416, 1557, 1644, 1726, 1864 |
| BstDSI | CCRYGG | 1 cut(s) | 371 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1283 |
| BstF5I | GGATG | 5 cut(s) | 1183, 1189, 1200, 1249, 1855 |
| BstFNI | CGCG | 1 cut(s) | 121 |
| BstHHI | GCGC | 3 cut(s) | 121, 1624, 2139 |
| BstKTI | GATC | 7 cut(s) | 80, 1411, 1573, 1681, 1709, 1735, 1759 |
| BstMAI | GTCTC | 2 cut(s) | 839, 969 |
| BstMBI | GATC | 7 cut(s) | 77, 1408, 1570, 1678, 1706, 1732, 1756 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 376, 872, 1256, 1342, 1619 |
| BstNSI | RCATGY | 2 cut(s) | 1122, 1466 |
| BstSCI | CCNGG | 5 cut(s) | 1783, 1881, 1983, 1984, 2002 |
| BstSFI | CTRYAG | 4 cut(s) | 95, 979, 1683, 1908 |
| BstSLI | GKGCMC | 1 cut(s) | 135 |
| BstUI | CGCG | 1 cut(s) | 121 |
| BstV1I | GCAGC | 4 cut(s) | 379, 895, 1622, 1697 |
| BstV2I | GAAGAC | 2 cut(s) | 941, 1516 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1316 |
| Bsu15I | ATCGAT | 1 cut(s) | 466 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1338 |
| BsuI | GTATCC | 3 cut(s) | 196, 1022, 1129 |
| BsuRI | GGCC | 6 cut(s) | 154, 1250, 1308, 1336, 1596, 2150 |
| BsuTUI | ATCGAT | 1 cut(s) | 466 |
| BtgI | CCRYGG | 1 cut(s) | 371 |
| BtsCI | GGATG | 5 cut(s) | 1183, 1189, 1200, 1249, 1855 |
| BtsI | GCAGTG | 3 cut(s) | 861, 1137, 1917 |
| BtsIMutI | CAGTG | 5 cut(s) | 861, 1137, 1245, 1566, 1917 |
| Cac8I | GCNNGC | 3 cut(s) | 152, 253, 873 |
| CfoI | GCGC | 3 cut(s) | 121, 1624, 2139 |
| Cfr13I | GGNCC | 4 cut(s) | 153, 1248, 1718, 1885 |
| Cfr9I | CCCGGG | 1 cut(s) | 1984 |
| ClaI | ATCGAT | 1 cut(s) | 466 |
| Csp6I | GTAC | 5 cut(s) | 4, 1164, 1433, 1991, 2038 |
| CviQI | GTAC | 5 cut(s) | 4, 1164, 1433, 1991, 2038 |
| DdeI | CTNAG | 7 cut(s) | 126, 1338, 1416, 1557, 1644, 1726, 1864 |
| DpnI | GATC | 7 cut(s) | 79, 1410, 1572, 1680, 1708, 1734, 1758 |
| DpnII | GATC | 7 cut(s) | 77, 1408, 1570, 1678, 1706, 1732, 1756 |
| DraI | TTTAAA | 2 cut(s) | 43, 763 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1520 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1520 |
| EaeI | YGGCCR | 3 cut(s) | 1306, 1594, 2148 |
| Eam1104I | CTCTTC | 6 cut(s) | 443, 492, 879, 1062, 1351, 1550 |
| EarI | CTCTTC | 6 cut(s) | 443, 492, 879, 1062, 1351, 1550 |
| EciI | GGCGGA | 1 cut(s) | 2022 |
| Ecl136II | GAGCTC | 2 cut(s) | 889, 1543 |
| Eco130I | CCWWGG | 4 cut(s) | 371, 795, 1056, 1597 |
| Eco24I | GRGCYC | 2 cut(s) | 891, 1545 |
| Eco47I | GGWCC | 2 cut(s) | 1718, 1885 |
| Eco53kI | GAGCTC | 2 cut(s) | 889, 1543 |
| Eco57I | CTGAAG | 2 cut(s) | 543, 1281 |
| Eco72I | CACGTG | 1 cut(s) | 815 |
| Eco81I | CCTNAGG | 1 cut(s) | 1338 |
| Eco88I | CYCGRG | 2 cut(s) | 712, 1984 |
| EcoICRI | GAGCTC | 2 cut(s) | 889, 1543 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1283 |
| EcoT14I | CCWWGG | 4 cut(s) | 371, 795, 1056, 1597 |
| EcoT22I | ATGCAT | 1 cut(s) | 355 |
| EcoT38I | GRGCYC | 2 cut(s) | 891, 1545 |
| ErhI | CCWWGG | 4 cut(s) | 371, 795, 1056, 1597 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 788, 820, 1254, 1286 |
| FaqI | GGGAC | 1 cut(s) | 596 |
| FbaI | TGATCA | 1 cut(s) | 1408 |
| FblI | GTMKAC | 1 cut(s) | 1857 |
| Fnu4HI | GCNGC | 4 cut(s) | 368, 909, 1611, 1686 |
| FokI | GGATG | 5 cut(s) | 1190, 1196, 1207, 1256, 1862 |
| FriOI | GRGCYC | 2 cut(s) | 891, 1545 |
| Fsp4HI | GCNGC | 4 cut(s) | 368, 909, 1611, 1686 |
| FspAI | RTGCGCAY | 1 cut(s) | 2138 |
| FspBI | CTAG | 6 cut(s) | 413, 494, 600, 809, 1280, 1488 |
| FspI | TGCGCA | 1 cut(s) | 2138 |
| GlaI | GCGC | 3 cut(s) | 120, 1623, 2138 |
| GluI | GCNGC | 4 cut(s) | 368, 909, 1611, 1686 |
| HaeIII | GGCC | 6 cut(s) | 154, 1250, 1308, 1336, 1596, 2150 |
| HapII | CCGG | 4 cut(s) | 1784, 1883, 1985, 2003 |
| HhaI | GCGC | 3 cut(s) | 121, 1624, 2139 |
| Hin6I | GCGC | 3 cut(s) | 119, 1622, 2137 |
| HinP1I | GCGC | 3 cut(s) | 119, 1622, 2137 |
| HincII | GTYRAC | 2 cut(s) | 285, 569 |
| HindII | GTYRAC | 2 cut(s) | 285, 569 |
| HindIII | AAGCTT | 1 cut(s) | 24 |
| HinfI | GANTC | 1 cut(s) | 2098 |
| HpaI | GTTAAC | 1 cut(s) | 285 |
| HpaII | CCGG | 4 cut(s) | 1784, 1883, 1985, 2003 |
| HphI | GGTGA | 6 cut(s) | 179, 622, 662, 733, 1072, 1301 |
| Hpy166II | GTNNAC | 4 cut(s) | 163, 285, 569, 1858 |
| Hpy188I | TCNGA | 4 cut(s) | 447, 771, 1000, 2103 |
| Hpy188III | TCNNGA | 7 cut(s) | 19, 274, 474, 704, 1046, 1076, 1710 |
| Hpy8I | GTNNAC | 4 cut(s) | 163, 285, 569, 1858 |
| HpyAV | CCTTC | 3 cut(s) | 518, 1210, 2038 |
| HpyCH4III | ACNGT | 5 cut(s) | 99, 167, 980, 1168, 1507 |
| HpyCH4IV | ACGT | 3 cut(s) | 159, 571, 814 |
| HpyCH4V | TGCA | 9 cut(s) | 353, 545, 866, 911, 1446, 1685, 1791, 1910, 2161 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 376, 872, 1256, 1342, 1619 |
| HpyF3I | CTNAG | 7 cut(s) | 126, 1338, 1416, 1557, 1644, 1726, 1864 |
| HpySE526I | ACGT | 3 cut(s) | 159, 571, 814 |
| HspAI | GCGC | 3 cut(s) | 119, 1622, 2137 |
| KpnI | GGTACC | 1 cut(s) | 1167 |
| Ksp22I | TGATCA | 1 cut(s) | 1408 |
| KspAI | GTTAAC | 1 cut(s) | 285 |
| Kzo9I | GATC | 7 cut(s) | 77, 1408, 1570, 1678, 1706, 1732, 1756 |
| LguI | GCTCTTC | 2 cut(s) | 879, 1550 |
| LmnI | GCTCC | 3 cut(s) | 306, 502, 1342 |
| Lsp1109I | GCAGC | 4 cut(s) | 379, 895, 1622, 1697 |
| LweI | GCATC | 1 cut(s) | 1800 |
| MaeI | CTAG | 6 cut(s) | 413, 494, 600, 809, 1280, 1488 |
| MaeII | ACGT | 3 cut(s) | 159, 571, 814 |
| MaeIII | GTNAC | 6 cut(s) | 46, 167, 610, 1530, 1564, 1931 |
| MalI | GATC | 7 cut(s) | 79, 1410, 1572, 1680, 1708, 1734, 1758 |
| MboI | GATC | 7 cut(s) | 77, 1408, 1570, 1678, 1706, 1732, 1756 |
| MfeI | CAATTG | 1 cut(s) | 1400 |
| MhlI | GDGCHC | 4 cut(s) | 135, 303, 891, 1545 |
| MlsI | TGGCCA | 3 cut(s) | 1308, 1596, 2150 |
| MluCI | AATT | 9 cut(s) | 380, 546, 789, 984, 1400, 1448, 1696, 1970, 2010 |
| MluNI | TGGCCA | 3 cut(s) | 1308, 1596, 2150 |
| MlyI | GAGTC | 1 cut(s) | 2092 |
| MmeI | TCCRAC | 5 cut(s) | 612, 712, 819, 1491, 1638 |
| Mox20I | TGGCCA | 3 cut(s) | 1308, 1596, 2150 |
| Mph1103I | ATGCAT | 1 cut(s) | 355 |
| MroXI | GAANNNNTTC | 2 cut(s) | 441, 1071 |
| MscI | TGGCCA | 3 cut(s) | 1308, 1596, 2150 |
| MseI | TTAA | 9 cut(s) | 42, 198, 284, 762, 893, 1188, 1926, 1973, 2055 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1629, 2144 |
| Msp20I | TGGCCA | 3 cut(s) | 1308, 1596, 2150 |
| MspI | CCGG | 4 cut(s) | 1784, 1883, 1985, 2003 |
| MspR9I | CCNGG | 5 cut(s) | 1785, 1883, 1985, 1986, 2004 |
| MunI | CAATTG | 1 cut(s) | 1400 |
| Mva1269I | GAATGC | 2 cut(s) | 269, 1924 |
| MvnI | CGCG | 1 cut(s) | 121 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 376, 872, 1256, 1342, 1619 |
| NciI | CCSGG | 5 cut(s) | 1785, 1883, 1985, 1986, 2004 |
| NcoI | CCATGG | 1 cut(s) | 371 |
| NdeII | GATC | 7 cut(s) | 77, 1408, 1570, 1678, 1706, 1732, 1756 |
| NlaIV | GGNNCC | 3 cut(s) | 132, 1165, 1249 |
| NmuCI | GTSAC | 4 cut(s) | 46, 167, 610, 1564 |
| NsbI | TGCGCA | 1 cut(s) | 2138 |
| NsiI | ATGCAT | 1 cut(s) | 355 |
| NspI | RCATGY | 2 cut(s) | 1122, 1466 |
| NspV | TTCGAA | 1 cut(s) | 1354 |
| PciI | ACATGT | 1 cut(s) | 1118 |
| PciSI | GCTCTTC | 2 cut(s) | 879, 1550 |
| PctI | GAATGC | 2 cut(s) | 269, 1924 |
| PdmI | GAANNNNTTC | 2 cut(s) | 441, 1071 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 970 |
| PkrI | GCNGC | 4 cut(s) | 369, 910, 1612, 1687 |
| PleI | GAGTC | 1 cut(s) | 2092 |
| PmaCI | CACGTG | 1 cut(s) | 815 |
| PmlI | CACGTG | 1 cut(s) | 815 |
| PpsI | GAGTC | 1 cut(s) | 2092 |
| Ppu21I | YACGTR | 1 cut(s) | 815 |
| PscI | ACATGT | 1 cut(s) | 1118 |
| PshBI | ATTAAT | 1 cut(s) | 2055 |
| Psp124BI | GAGCTC | 2 cut(s) | 891, 1545 |
| PspCI | CACGTG | 1 cut(s) | 815 |
| PspN4I | GGNNCC | 3 cut(s) | 132, 1165, 1249 |
| PspPI | GGNCC | 4 cut(s) | 153, 1248, 1718, 1885 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1015, 1047 |
| PstI | CTGCAG | 2 cut(s) | 1687, 1912 |
| RsaI | GTAC | 5 cut(s) | 5, 1165, 1434, 1992, 2039 |
| RsaNI | GTAC | 5 cut(s) | 4, 1164, 1433, 1991, 2038 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1629, 2144 |
| SacI | GAGCTC | 2 cut(s) | 891, 1545 |
| SapI | GCTCTTC | 2 cut(s) | 879, 1550 |
| SaqAI | TTAA | 9 cut(s) | 42, 198, 284, 762, 893, 1188, 1926, 1973, 2055 |
| SatI | GCNGC | 4 cut(s) | 368, 909, 1611, 1686 |
| Sau3AI | GATC | 7 cut(s) | 77, 1408, 1570, 1678, 1706, 1732, 1756 |
| Sau96I | GGNCC | 4 cut(s) | 153, 1248, 1718, 1885 |
| SchI | GAGTC | 1 cut(s) | 2092 |
| ScrFI | CCNGG | 5 cut(s) | 1785, 1883, 1985, 1986, 2004 |
| SduI | GDGCHC | 4 cut(s) | 135, 303, 891, 1545 |
| SfaNI | GCATC | 1 cut(s) | 1800 |
| SfcI | CTRYAG | 4 cut(s) | 95, 979, 1683, 1908 |
| SfuI | TTCGAA | 1 cut(s) | 1354 |
| SinI | GGWCC | 2 cut(s) | 1718, 1885 |
| SmaI | CCCGGG | 1 cut(s) | 1986 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1629, 2144 |
| SmlI | CTYRAG | 2 cut(s) | 656, 687 |
| SmoI | CTYRAG | 2 cut(s) | 656, 687 |
| SpeI | ACTAGT | 1 cut(s) | 1487 |
| Sse9I | AATT | 9 cut(s) | 380, 546, 789, 984, 1400, 1448, 1696, 1970, 2010 |
| SsiI | CCGC | 5 cut(s) | 121, 1922, 2007, 2035, 2090 |
| SspMI | CTAG | 6 cut(s) | 413, 494, 600, 809, 1280, 1488 |
| SstI | GAGCTC | 2 cut(s) | 891, 1545 |
| StyD4I | CCNGG | 5 cut(s) | 1783, 1881, 1983, 1984, 2002 |
| StyI | CCWWGG | 4 cut(s) | 371, 795, 1056, 1597 |
| TaaI | ACNGT | 5 cut(s) | 99, 167, 980, 1168, 1507 |
| TaiI | ACGT | 3 cut(s) | 162, 574, 817 |
| TaqI | TCGA | 4 cut(s) | 192, 466, 1354, 2078 |
| TasI | AATT | 9 cut(s) | 380, 546, 789, 984, 1400, 1448, 1696, 1970, 2010 |
| TatI | WGTACW | 1 cut(s) | 3 |
| Tru1I | TTAA | 9 cut(s) | 42, 198, 284, 762, 893, 1188, 1926, 1973, 2055 |
| Tru9I | TTAA | 9 cut(s) | 42, 198, 284, 762, 893, 1188, 1926, 1973, 2055 |
| TscAI | CASTG | 5 cut(s) | 868, 1137, 1245, 1573, 1917 |
| TseFI | GTSAC | 4 cut(s) | 46, 167, 610, 1564 |
| TseI | GCWGC | 4 cut(s) | 367, 908, 1610, 1685 |
| Tsp45I | GTSAC | 4 cut(s) | 46, 167, 610, 1564 |
| TspGWI | ACGGA | 1 cut(s) | 1200 |
| TspMI | CCCGGG | 1 cut(s) | 1984 |
| TspRI | CASTG | 5 cut(s) | 868, 1137, 1245, 1573, 1917 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 970 |
| VpaK11BI | GGWCC | 2 cut(s) | 1718, 1885 |
| VspI | ATTAAT | 1 cut(s) | 2055 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1283 |
| XapI | RAATTY | 1 cut(s) | 984 |
| XceI | RCATGY | 2 cut(s) | 1122, 1466 |
| XmaI | CCCGGG | 1 cut(s) | 1984 |
| XmiI | GTMKAC | 1 cut(s) | 1857 |
| XmnI | GAANNNNTTC | 2 cut(s) | 441, 1071 |
| XspI | CTAG | 6 cut(s) | 413, 494, 600, 809, 1280, 1488 |
| Zsp2I | ATGCAT | 1 cut(s) | 355 |