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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 1457 |
| Acc16I | TGCGCA | 1 cut(s) | 1946 |
| Acc65I | GGTACC | 1 cut(s) | 1097 |
| AccB1I | GGYRCC | 3 cut(s) | 307, 1097, 1870 |
| AccII | CGCG | 1 cut(s) | 298 |
| AciI | CCGC | 2 cut(s) | 1220, 1829 |
| AclWI | GGATC | 1 cut(s) | 1610 |
| AcoI | YGGCCR | 1 cut(s) | 1531 |
| AcsI | RAATTY | 3 cut(s) | 360, 557, 970 |
| AcvI | CACGTG | 2 cut(s) | 785, 1261 |
| AfaI | GTAC | 4 cut(s) | 182, 1099, 1394, 1470 |
| AfeI | AGCGCT | 1 cut(s) | 1379 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 488, 1219, 1220, 1807 |
| AgeI | ACCGGT | 1 cut(s) | 1671 |
| AhlI | ACTAGT | 1 cut(s) | 1424 |
| AjnI | CCWGG | 2 cut(s) | 375, 1720 |
| AluBI | AGCT | 9 cut(s) | 56, 327, 625, 736, 1142, 1279, 1352, 1387, 1815 |
| AluI | AGCT | 9 cut(s) | 56, 327, 625, 736, 1142, 1279, 1352, 1387, 1815 |
| Alw21I | GWGCWC | 1 cut(s) | 1817 |
| Alw26I | GTCTC | 5 cut(s) | 77, 436, 809, 1285, 1581 |
| AlwI | GGATC | 1 cut(s) | 1610 |
| Ama87I | CYCGRG | 2 cut(s) | 74, 682 |
| Aor51HI | AGCGCT | 1 cut(s) | 1379 |
| AoxI | GGCC | 5 cut(s) | 329, 393, 1268, 1531, 1557 |
| ApeKI | GCWGC | 6 cut(s) | 478, 1142, 1380, 1547, 1622, 1898 |
| ApoI | RAATTY | 3 cut(s) | 360, 557, 970 |
| AseI | ATTAAT | 2 cut(s) | 776, 1122 |
| AsiGI | ACCGGT | 1 cut(s) | 1671 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 1023, 1236 |
| Asp718I | GGTACC | 1 cut(s) | 1097 |
| AspLEI | GCGC | 3 cut(s) | 298, 1380, 1947 |
| AspS9I | GGNCC | 3 cut(s) | 394, 1558, 1774 |
| AsuHPI | GGTGA | 7 cut(s) | 632, 703, 1069, 1193, 1205, 1235, 1831 |
| AvaI | CYCGRG | 2 cut(s) | 74, 682 |
| AvaII | GGWCC | 1 cut(s) | 1774 |
| AxyI | CCTNAGG | 1 cut(s) | 1272 |
| BaeGI | GKGCMC | 1 cut(s) | 1374 |
| BalI | TGGCCA | 1 cut(s) | 1533 |
| BanI | GGYRCC | 3 cut(s) | 307, 1097, 1870 |
| BanII | GRGCYC | 1 cut(s) | 1817 |
| BbrPI | CACGTG | 2 cut(s) | 785, 1261 |
| BbsI | GAAGAC | 2 cut(s) | 71, 581 |
| Bbv12I | GWGCWC | 1 cut(s) | 1817 |
| BbvCI | CCTCAGC | 1 cut(s) | 1353 |
| BbvI | GCAGC | 6 cut(s) | 465, 1129, 1367, 1559, 1609, 1910 |
| BccI | CCATC | 4 cut(s) | 1082, 1172, 1222, 1306 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1586, 1620 |
| BciT130I | CCWGG | 2 cut(s) | 377, 1722 |
| BciVI | GTATCC | 1 cut(s) | 373 |
| BclI | TGATCA | 1 cut(s) | 192 |
| BcoDI | GTCTC | 5 cut(s) | 77, 436, 809, 1285, 1581 |
| BcuI | ACTAGT | 1 cut(s) | 1424 |
| BfaI | CTAG | 4 cut(s) | 626, 665, 884, 1425 |
| BfmI | CTRYAG | 3 cut(s) | 333, 1215, 1979 |
| BfoI | RGCGCY | 1 cut(s) | 1381 |
| BfuI | GTATCC | 1 cut(s) | 373 |
| BglII | AGATCT | 1 cut(s) | 856 |
| BisI | GCNGC | 6 cut(s) | 479, 1143, 1381, 1548, 1623, 1899 |
| BlsI | GCNGC | 6 cut(s) | 480, 1144, 1382, 1549, 1624, 1900 |
| Bme1390I | CCNGG | 2 cut(s) | 377, 1722 |
| Bme18I | GGWCC | 1 cut(s) | 1774 |
| BmeT110I | CYCGRG | 2 cut(s) | 74, 682 |
| BmgT120I | GGNCC | 3 cut(s) | 394, 1558, 1774 |
| BmiI | GGNNCC | 7 cut(s) | 7, 309, 396, 1099, 1657, 1776, 1872 |
| BmrFI | CCNGG | 2 cut(s) | 377, 1722 |
| BmsI | GCATC | 2 cut(s) | 1148, 1186 |
| BpiI | GAAGAC | 2 cut(s) | 71, 581 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1353 |
| BpuEI | CTTGAG | 1 cut(s) | 642 |
| BsaAI | YACGTR | 2 cut(s) | 785, 1261 |
| BsaJI | CCNNGG | 5 cut(s) | 548, 765, 1534, 1721, 1892 |
| BsaWI | WCCGGW | 1 cut(s) | 1671 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 696, 726 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 488, 1219, 1220, 1807 |
| Bse118I | RCCGGY | 2 cut(s) | 428, 1671 |
| Bse1I | ACTGG | 1 cut(s) | 135 |
| Bse21I | CCTNAGG | 1 cut(s) | 1272 |
| BseBI | CCWGG | 2 cut(s) | 377, 1722 |
| BseDI | CCNNGG | 5 cut(s) | 548, 765, 1534, 1721, 1892 |
| BseGI | GGATG | 5 cut(s) | 598, 1015, 1163, 1183, 1762 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 488, 1219, 1220, 1807 |
| BseMII | CTCAG | 4 cut(s) | 497, 581, 1263, 1344 |
| BseNI | ACTGG | 1 cut(s) | 135 |
| BseRI | GAGGAG | 2 cut(s) | 827, 1289 |
| BseSI | GKGCMC | 1 cut(s) | 1374 |
| BseXI | GCAGC | 6 cut(s) | 465, 1129, 1367, 1559, 1609, 1910 |
| Bsh1236I | CGCG | 1 cut(s) | 298 |
| BshFI | GGCC | 5 cut(s) | 331, 395, 1270, 1533, 1559 |
| BshNI | GGYRCC | 3 cut(s) | 307, 1097, 1870 |
| BshTI | ACCGGT | 1 cut(s) | 1671 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1817 |
| BsiHKCI | CYCGRG | 2 cut(s) | 74, 682 |
| BsiSI | CCGG | 3 cut(s) | 429, 1619, 1672 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 488, 1219, 1220, 1807 |
| BsmAI | GTCTC | 5 cut(s) | 77, 436, 809, 1285, 1581 |
| BsmBI | CGTCTC | 1 cut(s) | 1581 |
| BsmI | GAATGC | 2 cut(s) | 294, 530 |
| BsnI | GGCC | 5 cut(s) | 331, 395, 1270, 1533, 1559 |
| BsoBI | CYCGRG | 2 cut(s) | 74, 682 |
| Bsp1286I | GDGCHC | 2 cut(s) | 1374, 1817 |
| Bsp1407I | TGTACA | 2 cut(s) | 180, 1392 |
| Bsp143I | GATC | 7 cut(s) | 192, 312, 799, 856, 1615, 1643, 1693 |
| Bsp19I | CCATGG | 1 cut(s) | 548 |
| BspACI | CCGC | 2 cut(s) | 1220, 1829 |
| BspANI | GGCC | 5 cut(s) | 331, 395, 1270, 1533, 1559 |
| BspCNI | CTCAG | 4 cut(s) | 498, 580, 1264, 1345 |
| BspFNI | CGCG | 1 cut(s) | 298 |
| BspHI | TCATGA | 2 cut(s) | 952, 1413 |
| BspLI | GGNNCC | 7 cut(s) | 7, 309, 396, 1099, 1657, 1776, 1872 |
| BspMAI | CTGCAG | 1 cut(s) | 337 |
| BspPI | GGATC | 1 cut(s) | 1610 |
| BspT107I | GGYRCC | 3 cut(s) | 307, 1097, 1870 |
| BsrFI | RCCGGY | 2 cut(s) | 428, 1671 |
| BsrGI | TGTACA | 2 cut(s) | 180, 1392 |
| BsrI | ACTGG | 1 cut(s) | 135 |
| BssAI | RCCGGY | 2 cut(s) | 428, 1671 |
| BssECI | CCNNGG | 5 cut(s) | 548, 765, 1534, 1721, 1892 |
| BssMI | GATC | 7 cut(s) | 192, 312, 799, 856, 1615, 1643, 1693 |
| BssT1I | CCWWGG | 3 cut(s) | 548, 765, 1534 |
| Bst2UI | CCWGG | 2 cut(s) | 377, 1722 |
| Bst4CI | ACNGT | 4 cut(s) | 127, 1102, 1444, 1637 |
| Bst6I | CTCTTC | 2 cut(s) | 605, 1487 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 271, 478, 1975 |
| BstAUI | TGTACA | 2 cut(s) | 180, 1392 |
| BstBAI | YACGTR | 2 cut(s) | 785, 1261 |
| BstC8I | GCNNGC | 4 cut(s) | 294, 329, 333, 1385 |
| BstDEI | CTNAG | 4 cut(s) | 506, 567, 1272, 1353 |
| BstDSI | CCRYGG | 2 cut(s) | 548, 1892 |
| BstF5I | GGATG | 5 cut(s) | 598, 1015, 1163, 1183, 1762 |
| BstFNI | CGCG | 1 cut(s) | 298 |
| BstH2I | RGCGCY | 1 cut(s) | 1381 |
| BstHHI | GCGC | 3 cut(s) | 298, 1380, 1947 |
| BstKTI | GATC | 7 cut(s) | 195, 315, 802, 859, 1618, 1646, 1696 |
| BstMAI | GTCTC | 5 cut(s) | 77, 436, 809, 1285, 1581 |
| BstMBI | GATC | 7 cut(s) | 192, 312, 799, 856, 1615, 1643, 1693 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 271, 478, 520, 742, 914, 1276, 1384, 1556, 1975 |
| BstNI | CCWGG | 2 cut(s) | 377, 1722 |
| BstNSI | RCATGY | 2 cut(s) | 476, 1403 |
| BstSCI | CCNGG | 2 cut(s) | 375, 1720 |
| BstSFI | CTRYAG | 3 cut(s) | 333, 1215, 1979 |
| BstSLI | GKGCMC | 1 cut(s) | 1374 |
| BstUI | CGCG | 1 cut(s) | 298 |
| BstV1I | GCAGC | 6 cut(s) | 465, 1129, 1367, 1559, 1609, 1910 |
| BstV2I | GAAGAC | 2 cut(s) | 71, 581 |
| BstX2I | RGATCY | 1 cut(s) | 856 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1447 |
| BstYI | RGATCY | 1 cut(s) | 856 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1272 |
| BsuI | GTATCC | 1 cut(s) | 373 |
| BsuRI | GGCC | 5 cut(s) | 331, 395, 1270, 1533, 1559 |
| BtgI | CCRYGG | 2 cut(s) | 548, 1892 |
| BtgZI | GCGATG | 1 cut(s) | 657 |
| BtsCI | GGATG | 5 cut(s) | 598, 1015, 1163, 1183, 1762 |
| BtsI | GCAGTG | 2 cut(s) | 342, 1071 |
| BtsIMutI | CAGTG | 6 cut(s) | 51, 142, 231, 342, 1071, 1179 |
| Cac8I | GCNNGC | 4 cut(s) | 294, 329, 333, 1385 |
| CciI | TCATGA | 2 cut(s) | 952, 1413 |
| CfoI | GCGC | 3 cut(s) | 298, 1380, 1947 |
| Cfr10I | RCCGGY | 2 cut(s) | 428, 1671 |
| Cfr13I | GGNCC | 3 cut(s) | 394, 1558, 1774 |
| Csp6I | GTAC | 4 cut(s) | 181, 1098, 1393, 1469 |
| CspAI | ACCGGT | 1 cut(s) | 1671 |
| CviAII | CATG | 9 cut(s) | 261, 473, 549, 953, 1400, 1414, 1555, 1568, 1806 |
| CviQI | GTAC | 4 cut(s) | 181, 1098, 1393, 1469 |
| DdeI | CTNAG | 4 cut(s) | 506, 567, 1272, 1353 |
| DpnI | GATC | 7 cut(s) | 194, 314, 801, 858, 1617, 1645, 1695 |
| DpnII | GATC | 7 cut(s) | 192, 312, 799, 856, 1615, 1643, 1693 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1457 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1457 |
| EaeI | YGGCCR | 1 cut(s) | 1531 |
| Eam1104I | CTCTTC | 2 cut(s) | 605, 1487 |
| EarI | CTCTTC | 2 cut(s) | 605, 1487 |
| Ecl136II | GAGCTC | 1 cut(s) | 1815 |
| Eco130I | CCWWGG | 3 cut(s) | 548, 765, 1534 |
| Eco24I | GRGCYC | 1 cut(s) | 1817 |
| Eco32I | GATATC | 1 cut(s) | 1762 |
| Eco47I | GGWCC | 1 cut(s) | 1774 |
| Eco47III | AGCGCT | 1 cut(s) | 1379 |
| Eco53kI | GAGCTC | 1 cut(s) | 1815 |
| Eco72I | CACGTG | 2 cut(s) | 785, 1261 |
| Eco81I | CCTNAGG | 1 cut(s) | 1272 |
| Eco88I | CYCGRG | 2 cut(s) | 74, 682 |
| EcoICRI | GAGCTC | 1 cut(s) | 1815 |
| EcoRII | CCWGG | 2 cut(s) | 375, 1720 |
| EcoRV | GATATC | 1 cut(s) | 1762 |
| EcoT14I | CCWWGG | 3 cut(s) | 548, 765, 1534 |
| EcoT22I | ATGCAT | 1 cut(s) | 532 |
| EcoT38I | GRGCYC | 1 cut(s) | 1817 |
| ErhI | CCWWGG | 3 cut(s) | 548, 765, 1534 |
| Esp3I | CGTCTC | 1 cut(s) | 1581 |
| FaeI | CATG | 9 cut(s) | 264, 476, 552, 956, 1403, 1417, 1558, 1571, 1809 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 443, 475 |
| FatI | CATG | 9 cut(s) | 260, 472, 548, 952, 1399, 1413, 1554, 1567, 1805 |
| FauI | CCCGC | 1 cut(s) | 1213 |
| FauNDI | CATATG | 2 cut(s) | 1052, 1748 |
| FbaI | TGATCA | 1 cut(s) | 192 |
| Fnu4HI | GCNGC | 6 cut(s) | 479, 1143, 1381, 1548, 1623, 1899 |
| FokI | GGATG | 5 cut(s) | 605, 1022, 1170, 1190, 1769 |
| FriOI | GRGCYC | 1 cut(s) | 1817 |
| Fsp4HI | GCNGC | 6 cut(s) | 479, 1143, 1381, 1548, 1623, 1899 |
| FspAI | RTGCGCAY | 1 cut(s) | 1946 |
| FspBI | CTAG | 4 cut(s) | 626, 665, 884, 1425 |
| FspI | TGCGCA | 1 cut(s) | 1946 |
| GlaI | GCGC | 3 cut(s) | 297, 1379, 1946 |
| GluI | GCNGC | 6 cut(s) | 479, 1143, 1381, 1548, 1623, 1899 |
| HaeII | RGCGCY | 1 cut(s) | 1381 |
| HaeIII | GGCC | 5 cut(s) | 331, 395, 1270, 1533, 1559 |
| HapII | CCGG | 3 cut(s) | 429, 1619, 1672 |
| HhaI | GCGC | 3 cut(s) | 298, 1380, 1947 |
| Hin1II | CATG | 9 cut(s) | 264, 476, 552, 956, 1403, 1417, 1558, 1571, 1809 |
| Hin6I | GCGC | 3 cut(s) | 296, 1378, 1945 |
| HinP1I | GCGC | 3 cut(s) | 296, 1378, 1945 |
| HincII | GTYRAC | 3 cut(s) | 420, 1360, 1669 |
| HindII | GTYRAC | 3 cut(s) | 420, 1360, 1669 |
| HindIII | AAGCTT | 1 cut(s) | 734 |
| HinfI | GANTC | 6 cut(s) | 77, 86, 107, 140, 1186, 1906 |
| HpaI | GTTAAC | 1 cut(s) | 1669 |
| HpaII | CCGG | 3 cut(s) | 429, 1619, 1672 |
| HphI | GGTGA | 7 cut(s) | 632, 703, 1069, 1193, 1205, 1235, 1831 |
| Hpy166II | GTNNAC | 6 cut(s) | 340, 420, 723, 1360, 1471, 1669 |
| Hpy188I | TCNGA | 8 cut(s) | 28, 82, 570, 758, 937, 1290, 1648, 1911 |
| Hpy188III | TCNNGA | 9 cut(s) | 74, 196, 451, 674, 803, 953, 1241, 1414, 1489 |
| Hpy8I | GTNNAC | 6 cut(s) | 340, 420, 723, 1360, 1471, 1669 |
| HpyAV | CCTTC | 3 cut(s) | 10, 547, 1144 |
| HpyCH4III | ACNGT | 4 cut(s) | 127, 1102, 1444, 1637 |
| HpyCH4IV | ACGT | 2 cut(s) | 784, 1260 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 271, 478, 520, 742, 914, 1276, 1384, 1556, 1975 |
| HpyF3I | CTNAG | 4 cut(s) | 506, 567, 1272, 1353 |
| HpySE526I | ACGT | 2 cut(s) | 784, 1260 |
| Hsp92II | CATG | 9 cut(s) | 264, 476, 552, 956, 1403, 1417, 1558, 1571, 1809 |
| HspAI | GCGC | 3 cut(s) | 296, 1378, 1945 |
| KpnI | GGTACC | 1 cut(s) | 1101 |
| Ksp22I | TGATCA | 1 cut(s) | 192 |
| KspAI | GTTAAC | 1 cut(s) | 1669 |
| Kzo9I | GATC | 7 cut(s) | 192, 312, 799, 856, 1615, 1643, 1693 |
| LmnI | GCTCC | 3 cut(s) | 639, 1139, 1276 |
| Lsp1109I | GCAGC | 6 cut(s) | 465, 1129, 1367, 1559, 1609, 1910 |
| LweI | GCATC | 2 cut(s) | 1148, 1186 |
| MaeI | CTAG | 4 cut(s) | 626, 665, 884, 1425 |
| MaeII | ACGT | 2 cut(s) | 784, 1260 |
| MaeIII | GTNAC | 6 cut(s) | 127, 223, 540, 562, 1256, 1576 |
| MalI | GATC | 7 cut(s) | 194, 314, 801, 858, 1617, 1645, 1695 |
| MboI | GATC | 7 cut(s) | 192, 312, 799, 856, 1615, 1643, 1693 |
| MflI | RGATCY | 1 cut(s) | 856 |
| MhlI | GDGCHC | 2 cut(s) | 1374, 1817 |
| MlsI | TGGCCA | 1 cut(s) | 1533 |
| MluNI | TGGCCA | 1 cut(s) | 1533 |
| MlyI | GAGTC | 3 cut(s) | 86, 95, 1900 |
| MmeI | TCCRAC | 3 cut(s) | 582, 682, 789 |
| Mox20I | TGGCCA | 1 cut(s) | 1533 |
| Mph1103I | ATGCAT | 1 cut(s) | 532 |
| MroXI | GAANNNNTTC | 2 cut(s) | 1023, 1236 |
| MscI | TGGCCA | 1 cut(s) | 1533 |
| MseI | TTAA | 7 cut(s) | 461, 732, 776, 863, 1122, 1668, 1833 |
| MslI | CAYNNNNRTG | 4 cut(s) | 287, 1425, 1566, 1952 |
| Msp20I | TGGCCA | 1 cut(s) | 1533 |
| MspI | CCGG | 3 cut(s) | 429, 1619, 1672 |
| MspR9I | CCNGG | 2 cut(s) | 377, 1722 |
| Mva1269I | GAATGC | 2 cut(s) | 294, 530 |
| MvaI | CCWGG | 2 cut(s) | 377, 1722 |
| MvnI | CGCG | 1 cut(s) | 298 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 271, 478, 520, 742, 914, 1276, 1384, 1556, 1975 |
| NcoI | CCATGG | 1 cut(s) | 548 |
| NdeI | CATATG | 2 cut(s) | 1052, 1748 |
| NdeII | GATC | 7 cut(s) | 192, 312, 799, 856, 1615, 1643, 1693 |
| NlaIII | CATG | 9 cut(s) | 264, 476, 552, 956, 1403, 1417, 1558, 1571, 1809 |
| NlaIV | GGNNCC | 7 cut(s) | 7, 309, 396, 1099, 1657, 1776, 1872 |
| NmuCI | GTSAC | 3 cut(s) | 127, 223, 1256 |
| NsbI | TGCGCA | 1 cut(s) | 1946 |
| NsiI | ATGCAT | 1 cut(s) | 532 |
| NspI | RCATGY | 2 cut(s) | 476, 1403 |
| PaeR7I | CTCGAG | 1 cut(s) | 74 |
| PagI | TCATGA | 2 cut(s) | 952, 1413 |
| PctI | GAATGC | 2 cut(s) | 294, 530 |
| PdmI | GAANNNNTTC | 2 cut(s) | 1023, 1236 |
| PfeI | GAWTC | 3 cut(s) | 107, 140, 1186 |
| PflFI | GACNNNGTC | 1 cut(s) | 125 |
| PfoI | TCCNGGA | 1 cut(s) | 375 |
| PinAI | ACCGGT | 1 cut(s) | 1671 |
| PkrI | GCNGC | 6 cut(s) | 480, 1144, 1382, 1549, 1624, 1900 |
| PleI | GAGTC | 3 cut(s) | 85, 94, 1900 |
| PmaCI | CACGTG | 2 cut(s) | 785, 1261 |
| PmlI | CACGTG | 2 cut(s) | 785, 1261 |
| PpsI | GAGTC | 3 cut(s) | 85, 94, 1900 |
| Ppu21I | YACGTR | 2 cut(s) | 785, 1261 |
| PshBI | ATTAAT | 2 cut(s) | 776, 1122 |
| Psp124BI | GAGCTC | 1 cut(s) | 1817 |
| Psp6I | CCWGG | 2 cut(s) | 375, 1720 |
| PspCI | CACGTG | 2 cut(s) | 785, 1261 |
| PspGI | CCWGG | 2 cut(s) | 375, 1720 |
| PspN4I | GGNNCC | 7 cut(s) | 7, 309, 396, 1099, 1657, 1776, 1872 |
| PspPI | GGNCC | 3 cut(s) | 394, 1558, 1774 |
| PstI | CTGCAG | 1 cut(s) | 337 |
| PsuI | RGATCY | 1 cut(s) | 856 |
| PsyI | GACNNNGTC | 1 cut(s) | 125 |
| RsaI | GTAC | 4 cut(s) | 182, 1099, 1394, 1470 |
| RsaNI | GTAC | 4 cut(s) | 181, 1098, 1393, 1469 |
| RseI | CAYNNNNRTG | 4 cut(s) | 287, 1425, 1566, 1952 |
| SacI | GAGCTC | 1 cut(s) | 1817 |
| SaqAI | TTAA | 7 cut(s) | 461, 732, 776, 863, 1122, 1668, 1833 |
| SatI | GCNGC | 6 cut(s) | 479, 1143, 1381, 1548, 1623, 1899 |
| Sau3AI | GATC | 7 cut(s) | 192, 312, 799, 856, 1615, 1643, 1693 |
| Sau96I | GGNCC | 3 cut(s) | 394, 1558, 1774 |
| SchI | GAGTC | 3 cut(s) | 86, 95, 1900 |
| ScrFI | CCNGG | 2 cut(s) | 377, 1722 |
| SduI | GDGCHC | 2 cut(s) | 1374, 1817 |
| SfaNI | GCATC | 2 cut(s) | 1148, 1186 |
| SfcI | CTRYAG | 3 cut(s) | 333, 1215, 1979 |
| Sfr274I | CTCGAG | 1 cut(s) | 74 |
| SinI | GGWCC | 1 cut(s) | 1774 |
| SlaI | CTCGAG | 1 cut(s) | 74 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 287, 1425, 1566, 1952 |
| SmlI | CTYRAG | 2 cut(s) | 74, 657 |
| SmoI | CTYRAG | 2 cut(s) | 74, 657 |
| SpeI | ACTAGT | 1 cut(s) | 1424 |
| SsiI | CCGC | 2 cut(s) | 1220, 1829 |
| SspMI | CTAG | 4 cut(s) | 626, 665, 884, 1425 |
| SstI | GAGCTC | 1 cut(s) | 1817 |
| StyD4I | CCNGG | 2 cut(s) | 375, 1720 |
| StyI | CCWWGG | 3 cut(s) | 548, 765, 1534 |
| TaaI | ACNGT | 4 cut(s) | 127, 1102, 1444, 1637 |
| TaiI | ACGT | 2 cut(s) | 787, 1263 |
| TaqI | TCGA | 4 cut(s) | 75, 1107, 1240, 1596 |
| TatI | WGTACW | 2 cut(s) | 180, 1392 |
| TfiI | GAWTC | 3 cut(s) | 107, 140, 1186 |
| Tru1I | TTAA | 7 cut(s) | 461, 732, 776, 863, 1122, 1668, 1833 |
| Tru9I | TTAA | 7 cut(s) | 461, 732, 776, 863, 1122, 1668, 1833 |
| TscAI | CASTG | 6 cut(s) | 51, 142, 238, 342, 1071, 1179 |
| TseFI | GTSAC | 3 cut(s) | 127, 223, 1256 |
| TseI | GCWGC | 6 cut(s) | 478, 1142, 1380, 1547, 1622, 1898 |
| Tsp45I | GTSAC | 3 cut(s) | 127, 223, 1256 |
| TspDTI | ATGAA | 6 cut(s) | 523, 609, 941, 1430, 1474, 1584 |
| TspGWI | ACGGA | 2 cut(s) | 708, 1881 |
| TspRI | CASTG | 6 cut(s) | 51, 142, 238, 342, 1071, 1179 |
| Tth111I | GACNNNGTC | 1 cut(s) | 125 |
| VpaK11BI | GGWCC | 1 cut(s) | 1774 |
| VspI | ATTAAT | 2 cut(s) | 776, 1122 |
| XapI | RAATTY | 3 cut(s) | 360, 557, 970 |
| XceI | RCATGY | 2 cut(s) | 476, 1403 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1728 |
| XhoI | CTCGAG | 1 cut(s) | 74 |
| XmnI | GAANNNNTTC | 2 cut(s) | 1023, 1236 |
| XspI | CTAG | 4 cut(s) | 626, 665, 884, 1425 |
| Zsp2I | ATGCAT | 1 cut(s) | 532 |