RchiOBHm_Chr3g0460901

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000290
Physical Location & Seq
Forward (+)
8985060 .. 8987484
2425 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42739

Sequence Viewer

Length: 1506 bp
ATGTGTTCCCTCATGGTTTTATTTGCTGCACAGAAACGAAAGTTGCCTGAAGGAGAGGTAATTGATCGGGCAGTTATGTGGAAGAAAGCCCGTATACCAAAAAATGGAAAGATAGATGAAGAACTCTCATTGGTGGCAGCAAAAATAGATGAACTGTTGGAGAAGAAGAGTAAAGGTGAGTTGGAAATCTCAGGGAGTAGTGATGTCTTGTCTCAAGCTCTAGACACTCCTGAACACTCGGGTAGGGTGAGGGGTGTTGGAGGCTTCGTTAACCCATCCTCCTACTTTAATCTGCCAAAGAAGAAAAGGATTCGAATTACAAAGGCAGATATGTTGGCCCATGATAAGGAGCGTGATAAAGAGATGGAGGACACAAAGAAAATGCTTCTTGCGCAGCAAGCAAAGACGGAAGCCATGTTATATCAAAGGATTGCACAACTAGAAGCACTAGTAACAGGGAAGACAGCCATGCATCCGCCTGCCACTGGGGATAATGTCATTTCCCCTATATCCGACAAAGGCAGCTTCCATGATAAATCAAGAATTGACAAATCTGATCATAATGATGCCAAGGTCATAGAAAATATGGAGGAATGTGATATTATACCTACTCCTGGAAAGGGTGCCGGGGTATGTGAGCTAGCAGTGGATAATATAAATAATATAGTTGCTTTCGGTTCCGTGTTCGAAGAGGGGGAACTAAGCAAGACACTGCATGGAGTTCCACTTAAGGAGGGAACTGTTCGTGTATCGGTGGATGGTATACTAAAACCAGATGCTGTGCTTCCTTTCCCCATCGAGGGTGAGATGGAATTCGTTAGGGAAGCTCTTGGGAGTCACGTAGCTTGGCCTGACGATCTTGTTGTGCGGACAATGGTTAAGAAAAAGAAGAAGAATCCTCCATTCTTCAAGTCATTGTTTGATCAAGTTGAGATTAATCCGTGGGTGCCGAAGCGCTGCAAGTTGTTGTATAAACATGCAACAACTATCATGAAAGAAACGGGGAGCTCAATAAGGATGGTGTTTTCTGAAGAAGTGTTTGGCGAACCCACACAACTCTTTGTCTTGTCTGAGAATGTGATTAATCTGTTGGAGATGCAGTGGATTGGCCAAGGAGTTATAGCAGCATATGAAGGGTACTTGCATGAACTTATTACTGAAAGGGACCTTTTGGACACCTTTGCTTTTGTTGATCCGGCAGCTACATACAATTGTGGAAGACCGGACTTTGTGCGGTACTTAGTTGATCGGTTAAAAGAGGGAAAGTCTGATCGTATTTTCTTTATGCCTTACAATCCGGGGCAGCATTGGATATTAACTATCCTATGGGAAGGTGAAATCTACATGTTGGATCCGTTGCCAAAACCAGTCCATTACAAAGAATGGGAGACCTCGGTGATGAATGCGGTGAAAACCTTCAATGCTGAAACTGGAAGGGTCAACAAACTGCCTAAAGTAAAACCGCTTCCGGTAAGGCTTATACATGTGTTTAATTGGCTGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 Transposase_24

Protein Analysis

501

Amino Acids

56.58

Weight (kDa)

6.67

Isoelectric Point (pI)

29.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8039 PF26133 211 - 289 4.7e-13 Domain of unknown function (DUF8039)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000290)

Species Orthologous Gene IDs
prunus_persica Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0352291 RchiOBHm_Chr1g0352301 RchiOBHm_Chr2g0145261 RchiOBHm_Chr2g0160781 RchiOBHm_Chr2g0160871 RchiOBHm_Chr3g0460901 RchiOBHm_Chr3g0494431 RchiOBHm_Chr3g0494441 RchiOBHm_Chr4g0385451 RchiOBHm_Chr4g0417051 RchiOBHm_Chr4g0424111 RchiOBHm_Chr5g0035911 RchiOBHm_Chr6g0269251 RchiOBHm_Chr6g0274141 RchiOBHm_Chr7g0191841 RchiOBHm_Chr7g0196691 RchiOBHm_Chr7g0211271 RchiOBHm_Chr7g0227321 RchiOBHm_Chr7g0242811
rosa_laevigata RLG00000013212
rosa_multiflora Rmu_co8387591.1_g000001 Rmu_sc0000147.1_g000094 Rmu_sc0000340.1_g000034 Rmu_sc0000546.1_g000048 Rmu_sc0001461.1_g000092 Rmu_sc0001659.1_g000042 Rmu_sc0002460.1_g000027 Rmu_sc0003170.1_g000021 Rmu_sc0004301.1_g000015 Rmu_sc0004344.1_g000009 Rmu_sc0005539.1_g000024 Rmu_sc0006803.1_g000018 Rmu_sc0006888.1_g000057 Rmu_sc0009330.1_g000004 Rmu_sc0010704.1_g000001 Rmu_sc0012787.1_g000002 Rmu_sc0023684.1_g000002 Rmu_ssc0000189.1_g000010
rosa_roxburghii Rroxscaffold_5G00377480 Rroxscaffold_6G00389290 Rroxscaffold_6G00414410 Rroxscaffold_7G00202010
rosa_rugosa Rorug01G0242100 Rorug02G0188500 Rorug02G0273900 Rorug03G0342600 Rorug05G0239000 Rorug07G0163300 Rorug07G0192100
rosa_samantha Rh1BG091000 Rh1BG200300 Rh1DG228100 Rh2AG268900 Rh2AG431600 Rh2AG508400 Rh2CG258700 Rh2CG417500 Rh2CG493900 Rh3AG323800 Rh3BG237800 Rh3CG306700 Rh3DG204700 Rh3DG259400 Rh4AG203100 Rh4AG377200 Rh4BG054300 Rh4BG125100 Rh4BG146000 Rh4BG251900 Rh4DG132500 Rh4DG155000 Rh4DG384000 Rh5BG230700 Rh5CG382700 Rh5CG532300 Rh5DG235600 Rh5DG259700 Rh5DG320000 Rh6AG162900 Rh6CG065400 Rh6CG065500 Rh6CG307600 Rh6CG310500 Rh6CG391900 Rh6DG062500 Rh6DG062600 Rh6DG187500 Rh7BG296200 Rh7DG266900 Rh7DG322300
rosa_wichuraiana Rw2G018510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 393
AccB1I GGYRCC 2 cut(s) 623, 946
AccB7I CCANNNNNTGG 1 cut(s) 104
AccI GTMKAC 2 cut(s) 94, 763
AciI CCGC 5 cut(s) 476, 868, 1234, 1406, 1463
AclWI GGATC 3 cut(s) 1187, 1346, 1359
AcoI YGGCCR 1 cut(s) 1108
AcsI RAATTY 1 cut(s) 812
AcuI CTGAAG 2 cut(s) 69, 1050
AfaI GTAC 2 cut(s) 1139, 1238
AfeI AGCGCT 1 cut(s) 956
AfiI CCNNNNNNNGG 7 cut(s) 104, 346, 485, 614, 620, 799, 800
AflII CTTAAG 1 cut(s) 728
AflIII ACRYGT 2 cut(s) 1344, 1483
AgsI TTSAA 2 cut(s) 910, 1420
AhlI ACTAGT 1 cut(s) 448
AjnI CCWGG 1 cut(s) 613
AjuI GAANNNNNNNTTGG 2 cut(s) 1023, 1055
AluBI AGCT 7 cut(s) 218, 525, 640, 827, 845, 1008, 1202
AluI AGCT 7 cut(s) 218, 525, 640, 827, 845, 1008, 1202
Alw21I GWGCWC 1 cut(s) 1010
Alw26I GTCTC 2 cut(s) 216, 1382
AlwI GGATC 3 cut(s) 1187, 1346, 1359
AlwNI CAGNNNCTG 1 cut(s) 779
Ama87I CYCGRG 1 cut(s) 238
Aor51HI AGCGCT 1 cut(s) 956
AoxI GGCC 3 cut(s) 336, 848, 1108
ApeKI GCWGC 8 cut(s) 26, 137, 394, 522, 957, 1124, 1199, 1303
ApoI RAATTY 1 cut(s) 812
AseI ATTAAT 2 cut(s) 936, 1083
Asp700I GAANNNNTTC 1 cut(s) 1415
AspLEI GCGC 2 cut(s) 394, 957
AspS9I GGNCC 2 cut(s) 337, 1165
AsuC2I CCSGG 2 cut(s) 628, 1299
AsuHPI GGTGA 6 cut(s) 188, 259, 815, 1346, 1408, 1420
AsuII TTCGAA 2 cut(s) 313, 687
AsuNHI GCTAGC 1 cut(s) 640
AvaI CYCGRG 1 cut(s) 238
AvaII GGWCC 1 cut(s) 1165
BalI TGGCCA 1 cut(s) 1110
BamHI GGATCC 1 cut(s) 1351
BanI GGYRCC 2 cut(s) 623, 946
BanII GRGCYC 1 cut(s) 1010
BarI GAAGNNNNNNTAC 2 cut(s) 1449, 1481
BbsI GAAGAC 2 cut(s) 467, 1225
Bbv12I GWGCWC 1 cut(s) 1010
BbvI GCAGC 8 cut(s) 13, 149, 406, 534, 944, 1136, 1211, 1315
BccI CCATC 6 cut(s) 283, 358, 752, 802, 803, 1012
BciT130I CCWGG 1 cut(s) 615
BclI TGATCA 2 cut(s) 556, 922
BcnI CCSGG 2 cut(s) 628, 1299
BcoDI GTCTC 2 cut(s) 216, 1382
BcuI ACTAGT 1 cut(s) 448
BfaI CTAG 4 cut(s) 221, 440, 449, 641
BfoI RGCGCY 1 cut(s) 958
BfrI CTTAAG 1 cut(s) 728
BisI GCNGC 8 cut(s) 27, 138, 395, 523, 958, 1125, 1200, 1304
BlsI GCNGC 8 cut(s) 28, 139, 396, 524, 959, 1126, 1201, 1305
Bme1390I CCNGG 3 cut(s) 615, 628, 1299
Bme18I GGWCC 1 cut(s) 1165
BmeT110I CYCGRG 1 cut(s) 238
BmgT120I GGNCC 2 cut(s) 337, 1165
BmiI GGNNCC 5 cut(s) 625, 679, 948, 1166, 1353
BmrFI CCNGG 3 cut(s) 615, 628, 1299
BmrI ACTGGG 1 cut(s) 495
BmsI GCATC 4 cut(s) 481, 556, 766, 1086
BmtI GCTAGC 1 cut(s) 644
BmuI ACTGGG 1 cut(s) 495
BpiI GAAGAC 2 cut(s) 467, 1225
Bpu14I TTCGAA 2 cut(s) 313, 687
BpuEI CTTGAG 1 cut(s) 198
BpuMI CCSGG 2 cut(s) 628, 1299
BsaAI YACGTR 1 cut(s) 841
BsaI GGTCTC 1 cut(s) 1382
BsaJI CCNNGG 6 cut(s) 570, 627, 941, 1111, 1298, 1392
BsaWI WCCGGW 2 cut(s) 1222, 1468
BsaXI ACNNNNNCTCC 6 cut(s) 252, 263, 282, 293, 1085, 1115
Bsc4I CCNNNNNNNGG 7 cut(s) 104, 346, 485, 614, 620, 799, 800
Bse1I ACTGG 3 cut(s) 490, 1367, 1435
BseBI CCWGG 1 cut(s) 615
BseDI CCNNGG 6 cut(s) 570, 627, 941, 1111, 1298, 1392
BseGI GGATG 4 cut(s) 275, 472, 763, 1023
BseLI CCNNNNNNNGG 7 cut(s) 104, 346, 485, 614, 620, 799, 800
BseMII CTCAG 3 cut(s) 204, 1062, 1490
BseNI ACTGG 3 cut(s) 490, 1367, 1435
BseXI GCAGC 8 cut(s) 13, 149, 406, 534, 944, 1136, 1211, 1315
BsgI GTGCAG 1 cut(s) 12
BshFI GGCC 3 cut(s) 338, 850, 1110
BshNI GGYRCC 2 cut(s) 623, 946
BsiHKAI GWGCWC 1 cut(s) 1010
BsiHKCI CYCGRG 1 cut(s) 238
BsiSI CCGG 5 cut(s) 627, 1196, 1223, 1298, 1469
BslFI GGGAC 1 cut(s) 1178
BslI CCNNNNNNNGG 7 cut(s) 104, 346, 485, 614, 620, 799, 800
BsmAI GTCTC 2 cut(s) 216, 1382
BsmFI GGGAC 1 cut(s) 1178
BsmI GAATGC 1 cut(s) 1408
BsnI GGCC 3 cut(s) 338, 850, 1110
Bso31I GGTCTC 1 cut(s) 1382
BsoBI CYCGRG 1 cut(s) 238
Bsp119I TTCGAA 2 cut(s) 313, 687
Bsp1286I GDGCHC 1 cut(s) 1010
Bsp143I GATC 8 cut(s) 64, 556, 856, 922, 1192, 1246, 1270, 1351
BspACI CCGC 5 cut(s) 476, 868, 1234, 1406, 1463
BspANI GGCC 3 cut(s) 338, 850, 1110
BspCNI CTCAG 3 cut(s) 203, 1063, 1491
BspHI TCATGA 1 cut(s) 990
BspLI GGNNCC 5 cut(s) 625, 679, 948, 1166, 1353
BspOI GCTAGC 1 cut(s) 644
BspPI GGATC 3 cut(s) 1187, 1346, 1359
BspT104I TTCGAA 2 cut(s) 313, 687
BspT107I GGYRCC 2 cut(s) 623, 946
BspTI CTTAAG 1 cut(s) 728
BspTNI GGTCTC 1 cut(s) 1382
BsrI ACTGG 3 cut(s) 490, 1367, 1435
BssECI CCNNGG 6 cut(s) 570, 627, 941, 1111, 1298, 1392
BssMI GATC 8 cut(s) 64, 556, 856, 922, 1192, 1246, 1270, 1351
BssNAI GTATAC 2 cut(s) 95, 764
BssT1I CCWWGG 2 cut(s) 570, 1111
Bst1107I GTATAC 2 cut(s) 95, 764
Bst2UI CCWGG 1 cut(s) 615
Bst4CI ACNGT 2 cut(s) 156, 742
Bst6I CTCTTC 2 cut(s) 161, 684
BstAFI CTTAAG 1 cut(s) 728
BstBAI YACGTR 1 cut(s) 841
BstBI TTCGAA 2 cut(s) 313, 687
BstC8I GCNNGC 3 cut(s) 399, 480, 642
BstDEI CTNAG 5 cut(s) 190, 701, 1071, 1240, 1499
BstDSI CCRYGG 1 cut(s) 941
BstF5I GGATG 4 cut(s) 275, 472, 763, 1023
BstH2I RGCGCY 1 cut(s) 958
BstHHI GCGC 2 cut(s) 394, 957
BstKTI GATC 8 cut(s) 67, 559, 859, 925, 1195, 1249, 1273, 1354
BstMAI GTCTC 2 cut(s) 216, 1382
BstMBI GATC 8 cut(s) 64, 556, 856, 922, 1192, 1246, 1270, 1351
BstMWI GCNNNNNNNGC 2 cut(s) 391, 398
BstNI CCWGG 1 cut(s) 615
BstNSI RCATGY 3 cut(s) 980, 1348, 1487
BstSCI CCNGG 3 cut(s) 613, 626, 1297
BstV1I GCAGC 8 cut(s) 13, 149, 406, 534, 944, 1136, 1211, 1315
BstV2I GAAGAC 2 cut(s) 467, 1225
BstX2I RGATCY 1 cut(s) 1351
BstYI RGATCY 1 cut(s) 1351
BstZ17I GTATAC 2 cut(s) 95, 764
BsuRI GGCC 3 cut(s) 338, 850, 1110
BtgI CCRYGG 1 cut(s) 941
BtsCI GGATG 4 cut(s) 275, 472, 763, 1023
BtsI GCAGTG 3 cut(s) 651, 710, 1106
BtsIMutI CAGTG 4 cut(s) 483, 651, 710, 1106
Cac8I GCNNGC 3 cut(s) 399, 480, 642
CaiI CAGNNNCTG 1 cut(s) 779
CciI TCATGA 1 cut(s) 990
CfoI GCGC 2 cut(s) 394, 957
Cfr13I GGNCC 2 cut(s) 337, 1165
Csp6I GTAC 2 cut(s) 1138, 1237
CviQI GTAC 2 cut(s) 1138, 1237
DdeI CTNAG 5 cut(s) 190, 701, 1071, 1240, 1499
DpnI GATC 8 cut(s) 66, 558, 858, 924, 1194, 1248, 1272, 1353
DpnII GATC 8 cut(s) 64, 556, 856, 922, 1192, 1246, 1270, 1351
EaeI YGGCCR 1 cut(s) 1108
Eam1104I CTCTTC 2 cut(s) 161, 684
EarI CTCTTC 2 cut(s) 161, 684
EciI GGCGGA 1 cut(s) 465
Ecl136II GAGCTC 1 cut(s) 1008
Eco130I CCWWGG 2 cut(s) 570, 1111
Eco24I GRGCYC 1 cut(s) 1010
Eco31I GGTCTC 1 cut(s) 1382
Eco47I GGWCC 1 cut(s) 1165
Eco47III AGCGCT 1 cut(s) 956
Eco53kI GAGCTC 1 cut(s) 1008
Eco57I CTGAAG 2 cut(s) 69, 1050
Eco88I CYCGRG 1 cut(s) 238
EcoICRI GAGCTC 1 cut(s) 1008
EcoO109I RGGNCCY 1 cut(s) 1165
EcoRI GAATTC 1 cut(s) 812
EcoRII CCWGG 1 cut(s) 613
EcoT14I CCWWGG 2 cut(s) 570, 1111
EcoT22I ATGCAT 1 cut(s) 474
EcoT38I GRGCYC 1 cut(s) 1010
ErhI CCWWGG 2 cut(s) 570, 1111
FaqI GGGAC 1 cut(s) 1178
FauNDI CATATG 1 cut(s) 1129
FbaI TGATCA 2 cut(s) 556, 922
FblI GTMKAC 2 cut(s) 94, 763
Fnu4HI GCNGC 8 cut(s) 27, 138, 395, 523, 958, 1125, 1200, 1304
FokI GGATG 4 cut(s) 262, 459, 770, 1030
FriOI GRGCYC 1 cut(s) 1010
Fsp4HI GCNGC 8 cut(s) 27, 138, 395, 523, 958, 1125, 1200, 1304
FspBI CTAG 4 cut(s) 221, 440, 449, 641
FspI TGCGCA 1 cut(s) 393
GlaI GCGC 2 cut(s) 393, 956
GluI GCNGC 8 cut(s) 27, 138, 395, 523, 958, 1125, 1200, 1304
HaeII RGCGCY 1 cut(s) 958
HaeIII GGCC 3 cut(s) 338, 850, 1110
HapII CCGG 5 cut(s) 627, 1196, 1223, 1298, 1469
HhaI GCGC 2 cut(s) 394, 957
Hin6I GCGC 2 cut(s) 392, 955
HinP1I GCGC 2 cut(s) 392, 955
HincII GTYRAC 2 cut(s) 271, 1441
HindII GTYRAC 2 cut(s) 271, 1441
HinfI GANTC 3 cut(s) 310, 835, 895
HpaI GTTAAC 1 cut(s) 271
HpaII CCGG 5 cut(s) 627, 1196, 1223, 1298, 1469
HphI GGTGA 6 cut(s) 188, 259, 815, 1346, 1408, 1420
Hpy166II GTNNAC 4 cut(s) 95, 271, 764, 1441
Hpy188I TCNGA 5 cut(s) 514, 556, 1030, 1072, 1270
Hpy188III TCNNGA 4 cut(s) 221, 230, 540, 991
Hpy8I GTNNAC 4 cut(s) 95, 271, 764, 1441
HpyAV CCTTC 5 cut(s) 44, 1127, 1325, 1426, 1428
HpyCH4III ACNGT 2 cut(s) 156, 742
HpyCH4IV ACGT 1 cut(s) 840
HpyCH4V TGCA 8 cut(s) 29, 434, 472, 715, 960, 980, 1099, 1144
HpyF10VI GCNNNNNNNGC 2 cut(s) 391, 398
HpyF3I CTNAG 5 cut(s) 190, 701, 1071, 1240, 1499
HpySE526I ACGT 1 cut(s) 840
HspAI GCGC 2 cut(s) 392, 955
Ksp22I TGATCA 2 cut(s) 556, 922
KspAI GTTAAC 1 cut(s) 271
Kzo9I GATC 8 cut(s) 64, 556, 856, 922, 1192, 1246, 1270, 1351
LmnI GCTCC 2 cut(s) 349, 1005
Lsp1109I GCAGC 8 cut(s) 13, 149, 406, 534, 944, 1136, 1211, 1315
LweI GCATC 4 cut(s) 481, 556, 766, 1086
MaeI CTAG 4 cut(s) 221, 440, 449, 641
MaeII ACGT 1 cut(s) 840
MaeIII GTNAC 2 cut(s) 451, 836
MalI GATC 8 cut(s) 66, 558, 858, 924, 1194, 1248, 1272, 1353
MboI GATC 8 cut(s) 64, 556, 856, 922, 1192, 1246, 1270, 1351
MfeI CAATTG 1 cut(s) 1210
MflI RGATCY 1 cut(s) 1351
MhlI GDGCHC 1 cut(s) 1010
MlsI TGGCCA 1 cut(s) 1110
MluCI AATT 6 cut(s) 60, 315, 543, 812, 1210, 1492
MluNI TGGCCA 1 cut(s) 1110
MlyI GAGTC 1 cut(s) 844
MmeI TCCRAC 6 cut(s) 138, 162, 238, 537, 1071, 1329
Mox20I TGGCCA 1 cut(s) 1110
Mph1103I ATGCAT 1 cut(s) 474
MroXI GAANNNNTTC 1 cut(s) 1415
MscI TGGCCA 1 cut(s) 1110
MseI TTAA 9 cut(s) 270, 288, 729, 879, 936, 1083, 1253, 1316, 1491
MslI CAYNNNNRTG 1 cut(s) 564
Msp20I TGGCCA 1 cut(s) 1110
MspCI CTTAAG 1 cut(s) 728
MspI CCGG 5 cut(s) 627, 1196, 1223, 1298, 1469
MspR9I CCNGG 3 cut(s) 615, 628, 1299
MunI CAATTG 1 cut(s) 1210
Mva1269I GAATGC 1 cut(s) 1408
MvaI CCWGG 1 cut(s) 615
MwoI GCNNNNNNNGC 2 cut(s) 391, 398
NciI CCSGG 2 cut(s) 628, 1299
NdeI CATATG 1 cut(s) 1129
NdeII GATC 8 cut(s) 64, 556, 856, 922, 1192, 1246, 1270, 1351
NheI GCTAGC 1 cut(s) 640
NlaIV GGNNCC 5 cut(s) 625, 679, 948, 1166, 1353
NmuCI GTSAC 1 cut(s) 836
NsbI TGCGCA 1 cut(s) 393
NsiI ATGCAT 1 cut(s) 474
NspI RCATGY 3 cut(s) 980, 1348, 1487
NspV TTCGAA 2 cut(s) 313, 687
PagI TCATGA 1 cut(s) 990
PciI ACATGT 2 cut(s) 1344, 1483
PctI GAATGC 1 cut(s) 1408
PdmI GAANNNNTTC 1 cut(s) 1415
PfeI GAWTC 2 cut(s) 310, 895
PflMI CCANNNNNTGG 1 cut(s) 104
PfoI TCCNGGA 1 cut(s) 613
PkrI GCNGC 8 cut(s) 28, 139, 396, 524, 959, 1126, 1201, 1305
PleI GAGTC 1 cut(s) 843
PpsI GAGTC 1 cut(s) 843
Ppu21I YACGTR 1 cut(s) 841
PpuMI RGGWCCY 1 cut(s) 1165
PscI ACATGT 2 cut(s) 1344, 1483
PshBI ATTAAT 2 cut(s) 936, 1083
Psp124BI GAGCTC 1 cut(s) 1010
Psp5II RGGWCCY 1 cut(s) 1165
Psp6I CCWGG 1 cut(s) 613
PspGI CCWGG 1 cut(s) 613
PspN4I GGNNCC 5 cut(s) 625, 679, 948, 1166, 1353
PspPI GGNCC 2 cut(s) 337, 1165
PspPPI RGGWCCY 1 cut(s) 1165
PstNI CAGNNNCTG 1 cut(s) 779
PsuI RGATCY 1 cut(s) 1351
RsaI GTAC 2 cut(s) 1139, 1238
RsaNI GTAC 2 cut(s) 1138, 1237
RseI CAYNNNNRTG 1 cut(s) 564
SacI GAGCTC 1 cut(s) 1010
SaqAI TTAA 9 cut(s) 270, 288, 729, 879, 936, 1083, 1253, 1316, 1491
SatI GCNGC 8 cut(s) 27, 138, 395, 523, 958, 1125, 1200, 1304
Sau3AI GATC 8 cut(s) 64, 556, 856, 922, 1192, 1246, 1270, 1351
Sau96I GGNCC 2 cut(s) 337, 1165
SchI GAGTC 1 cut(s) 844
ScrFI CCNGG 3 cut(s) 615, 628, 1299
SduI GDGCHC 1 cut(s) 1010
SfaNI GCATC 4 cut(s) 481, 556, 766, 1086
SfuI TTCGAA 2 cut(s) 313, 687
SinI GGWCC 1 cut(s) 1165
SmiMI CAYNNNNRTG 1 cut(s) 564
SmlI CTYRAG 2 cut(s) 213, 728
SmoI CTYRAG 2 cut(s) 213, 728
SpeI ACTAGT 1 cut(s) 448
Sse9I AATT 6 cut(s) 60, 315, 543, 812, 1210, 1492
SsiI CCGC 5 cut(s) 476, 868, 1234, 1406, 1463
SspMI CTAG 4 cut(s) 221, 440, 449, 641
SstI GAGCTC 1 cut(s) 1010
StyD4I CCNGG 3 cut(s) 613, 626, 1297
StyI CCWWGG 2 cut(s) 570, 1111
TaaI ACNGT 2 cut(s) 156, 742
TaiI ACGT 1 cut(s) 843
TaqI TCGA 3 cut(s) 313, 687, 798
TasI AATT 6 cut(s) 60, 315, 543, 812, 1210, 1492
TfiI GAWTC 2 cut(s) 310, 895
Tru1I TTAA 9 cut(s) 270, 288, 729, 879, 936, 1083, 1253, 1316, 1491
Tru9I TTAA 9 cut(s) 270, 288, 729, 879, 936, 1083, 1253, 1316, 1491
TscAI CASTG 4 cut(s) 490, 651, 717, 1106
TseFI GTSAC 1 cut(s) 836
TseI GCWGC 8 cut(s) 26, 137, 394, 522, 957, 1124, 1199, 1303
Tsp45I GTSAC 1 cut(s) 836
TspDTI ATGAA 6 cut(s) 132, 165, 1007, 1146, 1161, 1415
TspGWI ACGGA 4 cut(s) 422, 670, 930, 1344
TspRI CASTG 4 cut(s) 490, 651, 717, 1106
Van91I CCANNNNNTGG 1 cut(s) 104
Vha464I CTTAAG 1 cut(s) 728
VpaK11BI GGWCC 1 cut(s) 1165
VspI ATTAAT 2 cut(s) 936, 1083
XapI RAATTY 1 cut(s) 812
XbaI TCTAGA 1 cut(s) 220
XceI RCATGY 3 cut(s) 980, 1348, 1487
XmiI GTMKAC 2 cut(s) 94, 763
XmnI GAANNNNTTC 1 cut(s) 1415
XspI CTAG 4 cut(s) 221, 440, 449, 641
Zsp2I ATGCAT 1 cut(s) 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.