Rh4BG054300

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000290
Physical Location & Seq
Reverse (-)
9785328 .. 9791753
6426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG054300.1

Sequence Viewer

Length: 654 bp
ATGGGTTCCAAGAAGGGAATTCGAAAGTCACCAAGAGGCAAAAAATTAAAGAGAAAAGCTGATCTGGAGACTTCTCAACCTGAGACTGATGAAGTGCTTGAAGAAAAAGAAGAATATGTATCAGCCAACACTATCACGAGCACCGAATCAGTCAAAACCAGAGGGAAGCGTAATGTGGTAGCACTGTACAAGGTCTTGGTCAAGAAAGCTTTGGGAAAAAAGTTTAAAGTATCATACACCGAGACGGGCAATCCAAATGGAAGAATCAGACACACTCTACAGTCTTACATAGGAATGTTGGCGCGGACCAAGGTGCCCATCAATATTGTAAGCTGGCCCGAAGTGGATGGTGACTTGAAGGATAAACTTTGGCTCGATGTTCAGAGAAAGACGTTGCCCGAAGGAGAGGTCATTGATCGTGCAATTATGTGGAAGAAAGCCCGTCAACGAAAAGATGGGGACATAGATGAGGAGGCTAGAGCTGTGGCCACAAAGATATTTCATACTAAGTTCACAGGGGAATTGAAAAGGGAAATTCGATGTAATTTTATTGTTATTTCAAATTATCATTCAGTGCTACTCCTATCTGCATTCAGTGCAACTCACAATTGGGATTTGGATCTACATGACTATTCATCTGACCCATGTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 Transposase_24

Protein Analysis

217

Amino Acids

24.79

Weight (kDa)

9.55

Isoelectric Point (pI)

27.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000290)

Species Orthologous Gene IDs
prunus_persica Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0352291 RchiOBHm_Chr1g0352301 RchiOBHm_Chr2g0145261 RchiOBHm_Chr2g0160781 RchiOBHm_Chr2g0160871 RchiOBHm_Chr3g0460901 RchiOBHm_Chr3g0494431 RchiOBHm_Chr3g0494441 RchiOBHm_Chr4g0385451 RchiOBHm_Chr4g0417051 RchiOBHm_Chr4g0424111 RchiOBHm_Chr5g0035911 RchiOBHm_Chr6g0269251 RchiOBHm_Chr6g0274141 RchiOBHm_Chr7g0191841 RchiOBHm_Chr7g0196691 RchiOBHm_Chr7g0211271 RchiOBHm_Chr7g0227321 RchiOBHm_Chr7g0242811
rosa_laevigata RLG00000013212
rosa_multiflora Rmu_co8387591.1_g000001 Rmu_sc0000147.1_g000094 Rmu_sc0000340.1_g000034 Rmu_sc0000546.1_g000048 Rmu_sc0001461.1_g000092 Rmu_sc0001659.1_g000042 Rmu_sc0002460.1_g000027 Rmu_sc0003170.1_g000021 Rmu_sc0004301.1_g000015 Rmu_sc0004344.1_g000009 Rmu_sc0005539.1_g000024 Rmu_sc0006803.1_g000018 Rmu_sc0006888.1_g000057 Rmu_sc0009330.1_g000004 Rmu_sc0010704.1_g000001 Rmu_sc0012787.1_g000002 Rmu_sc0023684.1_g000002 Rmu_ssc0000189.1_g000010
rosa_roxburghii Rroxscaffold_5G00377480 Rroxscaffold_6G00389290 Rroxscaffold_6G00414410 Rroxscaffold_7G00202010
rosa_rugosa Rorug01G0242100 Rorug02G0188500 Rorug02G0273900 Rorug03G0342600 Rorug05G0239000 Rorug07G0163300 Rorug07G0192100
rosa_samantha Rh1BG091000 Rh1BG200300 Rh1DG228100 Rh2AG268900 Rh2AG431600 Rh2AG508400 Rh2CG258700 Rh2CG417500 Rh2CG493900 Rh3AG323800 Rh3BG237800 Rh3CG306700 Rh3DG204700 Rh3DG259400 Rh4AG203100 Rh4AG377200 Rh4BG054300 Rh4BG125100 Rh4BG146000 Rh4BG251900 Rh4DG132500 Rh4DG155000 Rh4DG384000 Rh5BG230700 Rh5CG382700 Rh5CG532300 Rh5DG235600 Rh5DG259700 Rh5DG320000 Rh6AG162900 Rh6CG065400 Rh6CG065500 Rh6CG307600 Rh6CG310500 Rh6CG391900 Rh6DG062500 Rh6DG062600 Rh6DG187500 Rh7BG296200 Rh7DG266900 Rh7DG322300
rosa_wichuraiana Rw2G018510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 313
AccII CGCG 1 cut(s) 304
AciI CCGC 1 cut(s) 304
AclWI GGATC 1 cut(s) 627
AcoI YGGCCR 1 cut(s) 486
AcsI RAATTY 2 cut(s) 18, 534
AfaI GTAC 1 cut(s) 188
AgsI TTSAA 4 cut(s) 101, 358, 526, 561
AluBI AGCT 4 cut(s) 59, 209, 333, 482
AluI AGCT 4 cut(s) 59, 209, 333, 482
Alw21I GWGCWC 1 cut(s) 143
Alw26I GTCTC 3 cut(s) 62, 77, 236
AlwI GGATC 1 cut(s) 627
AoxI GGCC 2 cut(s) 335, 486
ApoI RAATTY 2 cut(s) 18, 534
ArsI GACNNNNNNTTYG 2 cut(s) 393, 425
AspLEI GCGC 1 cut(s) 304
AspS9I GGNCC 2 cut(s) 306, 336
AsuHPI GGTGA 2 cut(s) 21, 362
AsuII TTCGAA 1 cut(s) 22
AvaII GGWCC 1 cut(s) 306
BaeGI GKGCMC 1 cut(s) 318
BalI TGGCCA 1 cut(s) 488
BanI GGYRCC 1 cut(s) 313
BauI CACGAG 1 cut(s) 136
Bbv12I GWGCWC 1 cut(s) 143
BccI CCATC 3 cut(s) 326, 341, 449
BcoDI GTCTC 3 cut(s) 62, 77, 236
BfaI CTAG 1 cut(s) 477
BfmI CTRYAG 1 cut(s) 278
Bme18I GGWCC 1 cut(s) 306
BmgT120I GGNCC 2 cut(s) 306, 336
BmiI GGNNCC 2 cut(s) 7, 315
BpmI CTGGAG 1 cut(s) 86
Bpu14I TTCGAA 1 cut(s) 22
BsaBI GATNNNNATC 1 cut(s) 618
BsaJI CCNNGG 1 cut(s) 309
Bse8I GATNNNNATC 1 cut(s) 618
BseDI CCNNGG 1 cut(s) 309
BseGI GGATG 1 cut(s) 352
BseJI GATNNNNATC 1 cut(s) 618
BseMII CTCAG 1 cut(s) 72
BseRI GAGGAG 1 cut(s) 485
BseSI GKGCMC 1 cut(s) 318
Bsh1236I CGCG 1 cut(s) 304
BshFI GGCC 2 cut(s) 337, 488
BshNI GGYRCC 1 cut(s) 313
BsiHKAI GWGCWC 1 cut(s) 143
BslFI GGGAC 1 cut(s) 473
BsmAI GTCTC 3 cut(s) 62, 77, 236
BsmBI CGTCTC 1 cut(s) 236
BsmFI GGGAC 1 cut(s) 473
BsmI GAATGC 1 cut(s) 590
BsnI GGCC 2 cut(s) 337, 488
Bsp119I TTCGAA 1 cut(s) 22
Bsp1286I GDGCHC 2 cut(s) 143, 318
Bsp1407I TGTACA 1 cut(s) 186
Bsp143I GATC 3 cut(s) 61, 415, 619
BspACI CCGC 1 cut(s) 304
BspANI GGCC 2 cut(s) 337, 488
BspCNI CTCAG 1 cut(s) 73
BspFNI CGCG 1 cut(s) 304
BspLI GGNNCC 2 cut(s) 7, 315
BspPI GGATC 1 cut(s) 627
BspT104I TTCGAA 1 cut(s) 22
BspT107I GGYRCC 1 cut(s) 313
BsrGI TGTACA 1 cut(s) 186
BssECI CCNNGG 1 cut(s) 309
BssMI GATC 3 cut(s) 61, 415, 619
BssSI CACGAG 1 cut(s) 136
BssT1I CCWWGG 1 cut(s) 309
Bst2BI CACGAG 1 cut(s) 136
Bst4CI ACNGT 2 cut(s) 186, 282
BstAPI GCANNNNNTGC 1 cut(s) 596
BstAUI TGTACA 1 cut(s) 186
BstBI TTCGAA 1 cut(s) 22
BstC8I GCNNGC 1 cut(s) 335
BstDEI CTNAG 2 cut(s) 81, 507
BstF5I GGATG 1 cut(s) 352
BstFNI CGCG 1 cut(s) 304
BstHHI GCGC 1 cut(s) 304
BstKTI GATC 3 cut(s) 64, 418, 622
BstMAI GTCTC 3 cut(s) 62, 77, 236
BstMBI GATC 3 cut(s) 61, 415, 619
BstMWI GCNNNNNNNGC 1 cut(s) 596
BstSFI CTRYAG 1 cut(s) 278
BstSLI GKGCMC 1 cut(s) 318
BstUI CGCG 1 cut(s) 304
BstX2I RGATCY 1 cut(s) 619
BstYI RGATCY 1 cut(s) 619
BsuRI GGCC 2 cut(s) 337, 488
BtsCI GGATG 1 cut(s) 352
BtsIMutI CAGTG 3 cut(s) 182, 579, 601
Cac8I GCNNGC 1 cut(s) 335
CfoI GCGC 1 cut(s) 304
Cfr13I GGNCC 2 cut(s) 306, 336
Csp6I GTAC 1 cut(s) 187
CviAII CATG 2 cut(s) 626, 645
CviQI GTAC 1 cut(s) 187
DdeI CTNAG 2 cut(s) 81, 507
DpnI GATC 3 cut(s) 63, 417, 621
DpnII GATC 3 cut(s) 61, 415, 619
DraI TTTAAA 1 cut(s) 226
EaeI YGGCCR 1 cut(s) 486
Eco130I CCWWGG 1 cut(s) 309
Eco47I GGWCC 1 cut(s) 306
EcoRI GAATTC 1 cut(s) 18
EcoT14I CCWWGG 1 cut(s) 309
ErhI CCWWGG 1 cut(s) 309
Esp3I CGTCTC 1 cut(s) 236
FaeI CATG 2 cut(s) 629, 648
FaiI YATR 8 cut(s) 117, 235, 290, 428, 464, 504, 627, 646
FaqI GGGAC 1 cut(s) 473
FatI CATG 2 cut(s) 625, 644
FokI GGATG 1 cut(s) 359
FspBI CTAG 1 cut(s) 477
GlaI GCGC 1 cut(s) 303
GsuI CTGGAG 1 cut(s) 86
HaeIII GGCC 2 cut(s) 337, 488
HhaI GCGC 1 cut(s) 304
Hin1II CATG 2 cut(s) 629, 648
Hin6I GCGC 1 cut(s) 302
HinP1I GCGC 1 cut(s) 302
HincII GTYRAC 1 cut(s) 446
HindII GTYRAC 1 cut(s) 446
HindIII AAGCTT 1 cut(s) 207
HinfI GANTC 2 cut(s) 146, 264
HphI GGTGA 2 cut(s) 21, 362
Hpy166II GTNNAC 2 cut(s) 446, 513
Hpy188I TCNGA 3 cut(s) 269, 384, 640
Hpy188III TCNNGA 3 cut(s) 65, 136, 202
Hpy8I GTNNAC 2 cut(s) 446, 513
HpyAV CCTTC 3 cut(s) 7, 352, 395
HpyCH4III ACNGT 2 cut(s) 186, 282
HpyCH4IV ACGT 1 cut(s) 392
HpyCH4V TGCA 3 cut(s) 422, 590, 599
HpyF10VI GCNNNNNNNGC 1 cut(s) 596
HpyF3I CTNAG 2 cut(s) 81, 507
HpySE526I ACGT 1 cut(s) 392
Hsp92II CATG 2 cut(s) 629, 648
HspAI GCGC 1 cut(s) 302
Kzo9I GATC 3 cut(s) 61, 415, 619
LpnPI CCDG 5 cut(s) 50, 93, 172, 319, 501
MaeI CTAG 1 cut(s) 477
MaeII ACGT 1 cut(s) 392
MaeIII GTNAC 2 cut(s) 27, 350
MalI GATC 3 cut(s) 63, 417, 621
MboI GATC 3 cut(s) 61, 415, 619
MboII GAAGA 4 cut(s) 113, 122, 273, 445
MfeI CAATTG 1 cut(s) 607
MflI RGATCY 1 cut(s) 619
MhlI GDGCHC 2 cut(s) 143, 318
MlsI TGGCCA 1 cut(s) 488
MluCI AATT 8 cut(s) 18, 44, 423, 521, 534, 544, 562, 607
MluNI TGGCCA 1 cut(s) 488
MnlI CCTC 5 cut(s) 29, 155, 400, 463, 466
Mox20I TGGCCA 1 cut(s) 488
MscI TGGCCA 1 cut(s) 488
MseI TTAA 2 cut(s) 47, 225
MslI CAYNNNNRTG 1 cut(s) 293
Msp20I TGGCCA 1 cut(s) 488
MunI CAATTG 1 cut(s) 607
Mva1269I GAATGC 1 cut(s) 590
MvnI CGCG 1 cut(s) 304
MwoI GCNNNNNNNGC 1 cut(s) 596
NdeII GATC 3 cut(s) 61, 415, 619
NlaIII CATG 2 cut(s) 629, 648
NlaIV GGNNCC 2 cut(s) 7, 315
NmuCI GTSAC 2 cut(s) 27, 350
NspV TTCGAA 1 cut(s) 22
PctI GAATGC 1 cut(s) 590
PfeI GAWTC 2 cut(s) 146, 264
PspN4I GGNNCC 2 cut(s) 7, 315
PspPI GGNCC 2 cut(s) 306, 336
PsuI RGATCY 1 cut(s) 619
RsaI GTAC 1 cut(s) 188
RsaNI GTAC 1 cut(s) 187
RseI CAYNNNNRTG 1 cut(s) 293
SaqAI TTAA 2 cut(s) 47, 225
Sau3AI GATC 3 cut(s) 61, 415, 619
Sau96I GGNCC 2 cut(s) 306, 336
SduI GDGCHC 2 cut(s) 143, 318
SetI ASST 9 cut(s) 61, 82, 195, 211, 315, 335, 395, 411, 484
SfcI CTRYAG 1 cut(s) 278
SfuI TTCGAA 1 cut(s) 22
SinI GGWCC 1 cut(s) 306
SmiMI CAYNNNNRTG 1 cut(s) 293
Sse9I AATT 8 cut(s) 18, 44, 423, 521, 534, 544, 562, 607
SsiI CCGC 1 cut(s) 304
SspI AATATT 1 cut(s) 325
SspMI CTAG 1 cut(s) 477
StyI CCWWGG 1 cut(s) 309
TaaI ACNGT 2 cut(s) 186, 282
TaiI ACGT 1 cut(s) 395
TaqI TCGA 3 cut(s) 22, 375, 538
TasI AATT 8 cut(s) 18, 44, 423, 521, 534, 544, 562, 607
TatI WGTACW 1 cut(s) 186
TfiI GAWTC 2 cut(s) 146, 264
Tru1I TTAA 2 cut(s) 47, 225
Tru9I TTAA 2 cut(s) 47, 225
TscAI CASTG 3 cut(s) 189, 579, 601
TseFI GTSAC 2 cut(s) 27, 350
Tsp45I GTSAC 2 cut(s) 27, 350
TspDTI ATGAA 3 cut(s) 105, 491, 624
TspRI CASTG 3 cut(s) 189, 579, 601
VpaK11BI GGWCC 1 cut(s) 306
XapI RAATTY 2 cut(s) 18, 534
XspI CTAG 1 cut(s) 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.