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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 815 |
| AccB1I | GGYRCC | 2 cut(s) | 307, 815 |
| AccI | GTMKAC | 1 cut(s) | 452 |
| AciI | CCGC | 1 cut(s) | 764 |
| AclWI | GGATC | 1 cut(s) | 1328 |
| AcoI | YGGCCR | 1 cut(s) | 1249 |
| AcsI | RAATTY | 2 cut(s) | 215, 360 |
| AcvI | CACGTG | 2 cut(s) | 515, 979 |
| AfaI | GTAC | 3 cut(s) | 261, 817, 1112 |
| AfeI | AGCGCT | 1 cut(s) | 1097 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 240, 937, 938 |
| AgeI | ACCGGT | 1 cut(s) | 1015 |
| AgsI | TTSAA | 5 cut(s) | 566, 868, 908, 1301, 1396 |
| AhlI | ACTAGT | 1 cut(s) | 1142 |
| Alw21I | GWGCWC | 1 cut(s) | 862 |
| Alw26I | GTCTC | 3 cut(s) | 59, 116, 539 |
| AlwI | GGATC | 1 cut(s) | 1328 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 110 |
| Aor51HI | AGCGCT | 1 cut(s) | 1097 |
| AoxI | GGCC | 2 cut(s) | 986, 1249 |
| ApeKI | GCWGC | 3 cut(s) | 727, 1265, 1340 |
| ApoI | RAATTY | 2 cut(s) | 215, 360 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1163, 1195 |
| AsiGI | ACCGGT | 1 cut(s) | 1015 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 954 |
| Asp718I | GGTACC | 1 cut(s) | 815 |
| AspLEI | GCGC | 2 cut(s) | 1098, 1279 |
| AsuC2I | CCSGG | 1 cut(s) | 1440 |
| AsuHPI | GGTGA | 4 cut(s) | 362, 911, 953, 1030 |
| AsuII | TTCGAA | 1 cut(s) | 1006 |
| AsuNHI | GCTAGC | 1 cut(s) | 292 |
| AxyI | CCTNAGG | 1 cut(s) | 990 |
| BaeGI | GKGCMC | 2 cut(s) | 312, 1092 |
| BalI | TGGCCA | 1 cut(s) | 1251 |
| BanI | GGYRCC | 2 cut(s) | 307, 815 |
| BanII | GRGCYC | 1 cut(s) | 862 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 576, 608 |
| BbrPI | CACGTG | 2 cut(s) | 515, 979 |
| BbsI | GAAGAC | 1 cut(s) | 1015 |
| Bbv12I | GWGCWC | 1 cut(s) | 862 |
| BbvI | GCAGC | 3 cut(s) | 714, 1277, 1327 |
| BccI | CCATC | 3 cut(s) | 890, 940, 1033 |
| BceAI | ACGGC | 1 cut(s) | 278 |
| BclI | TGATCA | 1 cut(s) | 192 |
| BcnI | CCSGG | 1 cut(s) | 1440 |
| BcoDI | GTCTC | 3 cut(s) | 59, 116, 539 |
| BcuI | ACTAGT | 1 cut(s) | 1142 |
| BfaI | CTAG | 5 cut(s) | 284, 293, 356, 395, 1143 |
| BfmI | CTRYAG | 1 cut(s) | 117 |
| BfoI | RGCGCY | 1 cut(s) | 1099 |
| BisI | GCNGC | 3 cut(s) | 728, 1266, 1341 |
| BlsI | GCNGC | 3 cut(s) | 729, 1267, 1342 |
| Bme1390I | CCNGG | 1 cut(s) | 1440 |
| BmiI | GGNNCC | 3 cut(s) | 7, 309, 817 |
| BmrFI | CCNGG | 1 cut(s) | 1440 |
| BmsI | GCATC | 2 cut(s) | 279, 904 |
| BmtI | GCTAGC | 1 cut(s) | 296 |
| BpiI | GAAGAC | 1 cut(s) | 1015 |
| BpmI | CTGGAG | 1 cut(s) | 560 |
| Bpu10I | CCTNAGC | 1 cut(s) | 471 |
| Bpu14I | TTCGAA | 1 cut(s) | 1006 |
| BpuEI | CTTGAG | 1 cut(s) | 372 |
| BpuMI | CCSGG | 1 cut(s) | 1440 |
| BsaAI | YACGTR | 2 cut(s) | 515, 979 |
| BsaI | GGTCTC | 1 cut(s) | 116 |
| BsaJI | CCNNGG | 2 cut(s) | 1252, 1439 |
| BsaWI | WCCGGW | 1 cut(s) | 1015 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 426, 456 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 240, 937, 938 |
| Bse118I | RCCGGY | 1 cut(s) | 1015 |
| Bse21I | CCTNAGG | 1 cut(s) | 990 |
| BseDI | CCNNGG | 2 cut(s) | 1252, 1439 |
| BseGI | GGATG | 4 cut(s) | 294, 489, 901, 1174 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 240, 937, 938 |
| BseMII | CTCAG | 3 cut(s) | 66, 981, 1203 |
| BseRI | GAGGAG | 2 cut(s) | 557, 1007 |
| BseSI | GKGCMC | 2 cut(s) | 312, 1092 |
| BseXI | GCAGC | 3 cut(s) | 714, 1277, 1327 |
| BshFI | GGCC | 2 cut(s) | 988, 1251 |
| BshNI | GGYRCC | 2 cut(s) | 307, 815 |
| BshTI | ACCGGT | 1 cut(s) | 1015 |
| BsiHKAI | GWGCWC | 1 cut(s) | 862 |
| BsiSI | CCGG | 2 cut(s) | 1016, 1439 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 240, 937, 938 |
| BsmAI | GTCTC | 3 cut(s) | 59, 116, 539 |
| BsnI | GGCC | 2 cut(s) | 988, 1251 |
| Bso31I | GGTCTC | 1 cut(s) | 116 |
| Bsp119I | TTCGAA | 1 cut(s) | 1006 |
| Bsp1286I | GDGCHC | 3 cut(s) | 312, 862, 1092 |
| Bsp1407I | TGTACA | 1 cut(s) | 1110 |
| Bsp143I | GATC | 8 cut(s) | 192, 529, 709, 1052, 1225, 1333, 1361, 1411 |
| BspACI | CCGC | 1 cut(s) | 764 |
| BspANI | GGCC | 2 cut(s) | 988, 1251 |
| BspCNI | CTCAG | 3 cut(s) | 67, 982, 1204 |
| BspHI | TCATGA | 1 cut(s) | 1131 |
| BspLI | GGNNCC | 3 cut(s) | 7, 309, 817 |
| BspMAI | CTGCAG | 1 cut(s) | 121 |
| BspOI | GCTAGC | 1 cut(s) | 296 |
| BspPI | GGATC | 1 cut(s) | 1328 |
| BspQI | GCTCTTC | 1 cut(s) | 579 |
| BspT104I | TTCGAA | 1 cut(s) | 1006 |
| BspT107I | GGYRCC | 2 cut(s) | 307, 815 |
| BspTNI | GGTCTC | 1 cut(s) | 116 |
| BsrFI | RCCGGY | 1 cut(s) | 1015 |
| BsrGI | TGTACA | 1 cut(s) | 1110 |
| BssAI | RCCGGY | 1 cut(s) | 1015 |
| BssECI | CCNNGG | 2 cut(s) | 1252, 1439 |
| BssMI | GATC | 8 cut(s) | 192, 529, 709, 1052, 1225, 1333, 1361, 1411 |
| BssT1I | CCWWGG | 1 cut(s) | 1252 |
| Bst4CI | ACNGT | 2 cut(s) | 820, 1162 |
| Bst6I | CTCTTC | 4 cut(s) | 11, 148, 579, 1205 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 271 |
| BstAUI | TGTACA | 1 cut(s) | 1110 |
| BstBAI | YACGTR | 2 cut(s) | 515, 979 |
| BstBI | TTCGAA | 1 cut(s) | 1006 |
| BstC8I | GCNNGC | 1 cut(s) | 294 |
| BstDEI | CTNAG | 7 cut(s) | 75, 471, 495, 990, 1212, 1231, 1381 |
| BstF5I | GGATG | 4 cut(s) | 294, 489, 901, 1174 |
| BstH2I | RGCGCY | 1 cut(s) | 1099 |
| BstHHI | GCGC | 2 cut(s) | 1098, 1279 |
| BstKTI | GATC | 8 cut(s) | 195, 532, 712, 1055, 1228, 1336, 1364, 1414 |
| BstMAI | GTCTC | 3 cut(s) | 59, 116, 539 |
| BstMBI | GATC | 8 cut(s) | 192, 529, 709, 1052, 1225, 1333, 1361, 1411 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 271, 472, 1274 |
| BstNSI | RCATGY | 1 cut(s) | 1121 |
| BstSCI | CCNGG | 1 cut(s) | 1438 |
| BstSFI | CTRYAG | 1 cut(s) | 117 |
| BstSLI | GKGCMC | 2 cut(s) | 312, 1092 |
| BstV1I | GCAGC | 3 cut(s) | 714, 1277, 1327 |
| BstV2I | GAAGAC | 1 cut(s) | 1015 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1165 |
| Bsu36I | CCTNAGG | 1 cut(s) | 990 |
| BsuRI | GGCC | 2 cut(s) | 988, 1251 |
| BtgZI | GCGATG | 1 cut(s) | 927 |
| BtsCI | GGATG | 4 cut(s) | 294, 489, 901, 1174 |
| BtsI | GCAGTG | 1 cut(s) | 789 |
| BtsIMutI | CAGTG | 3 cut(s) | 789, 897, 1167 |
| Cac8I | GCNNGC | 1 cut(s) | 294 |
| CaiI | CAGNNNCTG | 1 cut(s) | 110 |
| CciI | TCATGA | 1 cut(s) | 1131 |
| CfoI | GCGC | 2 cut(s) | 1098, 1279 |
| Cfr10I | RCCGGY | 1 cut(s) | 1015 |
| Csp6I | GTAC | 3 cut(s) | 260, 816, 1111 |
| CspAI | ACCGGT | 1 cut(s) | 1015 |
| CviAII | CATG | 6 cut(s) | 683, 854, 1118, 1132, 1273, 1286 |
| CviQI | GTAC | 3 cut(s) | 260, 816, 1111 |
| DdeI | CTNAG | 7 cut(s) | 75, 471, 495, 990, 1212, 1231, 1381 |
| DpnI | GATC | 8 cut(s) | 194, 531, 711, 1054, 1227, 1335, 1363, 1413 |
| DpnII | GATC | 8 cut(s) | 192, 529, 709, 1052, 1225, 1333, 1361, 1411 |
| EaeI | YGGCCR | 1 cut(s) | 1249 |
| Eam1104I | CTCTTC | 4 cut(s) | 11, 148, 579, 1205 |
| EarI | CTCTTC | 4 cut(s) | 11, 148, 579, 1205 |
| EciI | GGCGGA | 1 cut(s) | 753 |
| Ecl136II | GAGCTC | 1 cut(s) | 860 |
| Eco130I | CCWWGG | 1 cut(s) | 1252 |
| Eco24I | GRGCYC | 1 cut(s) | 862 |
| Eco31I | GGTCTC | 1 cut(s) | 116 |
| Eco47III | AGCGCT | 1 cut(s) | 1097 |
| Eco53kI | GAGCTC | 1 cut(s) | 860 |
| Eco72I | CACGTG | 2 cut(s) | 515, 979 |
| Eco81I | CCTNAGG | 1 cut(s) | 990 |
| EcoICRI | GAGCTC | 1 cut(s) | 860 |
| EcoT14I | CCWWGG | 1 cut(s) | 1252 |
| EcoT38I | GRGCYC | 1 cut(s) | 862 |
| ErhI | CCWWGG | 1 cut(s) | 1252 |
| FaeI | CATG | 6 cut(s) | 686, 857, 1121, 1135, 1276, 1289 |
| FatI | CATG | 6 cut(s) | 682, 853, 1117, 1131, 1272, 1285 |
| FbaI | TGATCA | 1 cut(s) | 192 |
| FblI | GTMKAC | 1 cut(s) | 452 |
| Fnu4HI | GCNGC | 3 cut(s) | 728, 1266, 1341 |
| FokI | GGATG | 4 cut(s) | 301, 496, 908, 1181 |
| FriOI | GRGCYC | 1 cut(s) | 862 |
| Fsp4HI | GCNGC | 3 cut(s) | 728, 1266, 1341 |
| FspBI | CTAG | 5 cut(s) | 284, 293, 356, 395, 1143 |
| GlaI | GCGC | 2 cut(s) | 1097, 1278 |
| GluI | GCNGC | 3 cut(s) | 728, 1266, 1341 |
| GsuI | CTGGAG | 1 cut(s) | 560 |
| HaeII | RGCGCY | 1 cut(s) | 1099 |
| HaeIII | GGCC | 2 cut(s) | 988, 1251 |
| HapII | CCGG | 2 cut(s) | 1016, 1439 |
| HhaI | GCGC | 2 cut(s) | 1098, 1279 |
| Hin1II | CATG | 6 cut(s) | 686, 857, 1121, 1135, 1276, 1289 |
| Hin6I | GCGC | 2 cut(s) | 1096, 1277 |
| HinP1I | GCGC | 2 cut(s) | 1096, 1277 |
| HincII | GTYRAC | 1 cut(s) | 721 |
| HindII | GTYRAC | 1 cut(s) | 721 |
| HindIII | AAGCTT | 2 cut(s) | 201, 464 |
| HinfI | GANTC | 5 cut(s) | 107, 113, 140, 244, 904 |
| HpaII | CCGG | 2 cut(s) | 1016, 1439 |
| HphI | GGTGA | 4 cut(s) | 362, 911, 953, 1030 |
| Hpy166II | GTNNAC | 2 cut(s) | 453, 721 |
| Hpy188I | TCNGA | 5 cut(s) | 76, 112, 300, 667, 1366 |
| Hpy188III | TCNNGA | 7 cut(s) | 196, 404, 533, 713, 959, 1132, 1207 |
| Hpy8I | GTNNAC | 2 cut(s) | 453, 721 |
| HpyAV | CCTTC | 3 cut(s) | 136, 340, 862 |
| HpyCH4III | ACNGT | 2 cut(s) | 820, 1162 |
| HpyCH4IV | ACGT | 2 cut(s) | 514, 978 |
| HpyCH4V | TGCA | 6 cut(s) | 119, 274, 730, 750, 917, 1240 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 271, 472, 1274 |
| HpyF3I | CTNAG | 7 cut(s) | 75, 471, 495, 990, 1212, 1231, 1381 |
| HpySE526I | ACGT | 2 cut(s) | 514, 978 |
| Hsp92II | CATG | 6 cut(s) | 686, 857, 1121, 1135, 1276, 1289 |
| HspAI | GCGC | 2 cut(s) | 1096, 1277 |
| KpnI | GGTACC | 1 cut(s) | 819 |
| Ksp22I | TGATCA | 1 cut(s) | 192 |
| Kzo9I | GATC | 8 cut(s) | 192, 529, 709, 1052, 1225, 1333, 1361, 1411 |
| LguI | GCTCTTC | 1 cut(s) | 579 |
| LmnI | GCTCC | 2 cut(s) | 857, 865 |
| LpnPI | CCDG | 7 cut(s) | 78, 351, 417, 524, 1002, 1029, 1350 |
| Lsp1109I | GCAGC | 3 cut(s) | 714, 1277, 1327 |
| LweI | GCATC | 2 cut(s) | 279, 904 |
| MaeI | CTAG | 5 cut(s) | 284, 293, 356, 395, 1143 |
| MaeII | ACGT | 2 cut(s) | 514, 978 |
| MaeIII | GTNAC | 2 cut(s) | 223, 974 |
| MalI | GATC | 8 cut(s) | 194, 531, 711, 1054, 1227, 1335, 1363, 1413 |
| MboI | GATC | 8 cut(s) | 192, 529, 709, 1052, 1225, 1333, 1361, 1411 |
| MboII | GAAGA | 8 cut(s) | 25, 28, 165, 554, 596, 1020, 1033, 1192 |
| MhlI | GDGCHC | 3 cut(s) | 312, 862, 1092 |
| MlsI | TGGCCA | 1 cut(s) | 1251 |
| MluNI | TGGCCA | 1 cut(s) | 1251 |
| MmeI | TCCRAC | 3 cut(s) | 412, 1146, 1293 |
| MnlI | CCTC | 9 cut(s) | 70, 149, 428, 535, 771, 865, 985, 1141, 1393 |
| Mox20I | TGGCCA | 1 cut(s) | 1251 |
| MroXI | GAANNNNTTC | 1 cut(s) | 954 |
| MscI | TGGCCA | 1 cut(s) | 1251 |
| MseI | TTAA | 4 cut(s) | 219, 315, 462, 840 |
| Msp20I | TGGCCA | 1 cut(s) | 1251 |
| MspI | CCGG | 2 cut(s) | 1016, 1439 |
| MspR9I | CCNGG | 1 cut(s) | 1440 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 271, 472, 1274 |
| NciI | CCSGG | 1 cut(s) | 1440 |
| NdeII | GATC | 8 cut(s) | 192, 529, 709, 1052, 1225, 1333, 1361, 1411 |
| NheI | GCTAGC | 1 cut(s) | 292 |
| NlaIII | CATG | 6 cut(s) | 686, 857, 1121, 1135, 1276, 1289 |
| NlaIV | GGNNCC | 3 cut(s) | 7, 309, 817 |
| NmuCI | GTSAC | 2 cut(s) | 223, 974 |
| NspI | RCATGY | 1 cut(s) | 1121 |
| NspV | TTCGAA | 1 cut(s) | 1006 |
| PagI | TCATGA | 1 cut(s) | 1131 |
| PciSI | GCTCTTC | 1 cut(s) | 579 |
| PdmI | GAANNNNTTC | 1 cut(s) | 954 |
| PfeI | GAWTC | 5 cut(s) | 107, 113, 140, 244, 904 |
| PflFI | GACNNNGTC | 1 cut(s) | 1175 |
| PinAI | ACCGGT | 1 cut(s) | 1015 |
| PkrI | GCNGC | 3 cut(s) | 729, 1267, 1342 |
| PmaCI | CACGTG | 2 cut(s) | 515, 979 |
| PmlI | CACGTG | 2 cut(s) | 515, 979 |
| Ppu21I | YACGTR | 2 cut(s) | 515, 979 |
| Psp124BI | GAGCTC | 1 cut(s) | 862 |
| PspCI | CACGTG | 2 cut(s) | 515, 979 |
| PspN4I | GGNNCC | 3 cut(s) | 7, 309, 817 |
| PstI | CTGCAG | 1 cut(s) | 121 |
| PstNI | CAGNNNCTG | 1 cut(s) | 110 |
| PsyI | GACNNNGTC | 1 cut(s) | 1175 |
| RsaI | GTAC | 3 cut(s) | 261, 817, 1112 |
| RsaNI | GTAC | 3 cut(s) | 260, 816, 1111 |
| SacI | GAGCTC | 1 cut(s) | 862 |
| SapI | GCTCTTC | 1 cut(s) | 579 |
| SaqAI | TTAA | 4 cut(s) | 219, 315, 462, 840 |
| SatI | GCNGC | 3 cut(s) | 728, 1266, 1341 |
| Sau3AI | GATC | 8 cut(s) | 192, 529, 709, 1052, 1225, 1333, 1361, 1411 |
| ScrFI | CCNGG | 1 cut(s) | 1440 |
| SduI | GDGCHC | 3 cut(s) | 312, 862, 1092 |
| SfaNI | GCATC | 2 cut(s) | 279, 904 |
| SfcI | CTRYAG | 1 cut(s) | 117 |
| SfuI | TTCGAA | 1 cut(s) | 1006 |
| SmlI | CTYRAG | 1 cut(s) | 387 |
| SmoI | CTYRAG | 1 cut(s) | 387 |
| SpeI | ACTAGT | 1 cut(s) | 1142 |
| SsiI | CCGC | 1 cut(s) | 764 |
| SspMI | CTAG | 5 cut(s) | 284, 293, 356, 395, 1143 |
| SstI | GAGCTC | 1 cut(s) | 862 |
| StyD4I | CCNGG | 1 cut(s) | 1438 |
| StyI | CCWWGG | 1 cut(s) | 1252 |
| TaaI | ACNGT | 2 cut(s) | 820, 1162 |
| TaiI | ACGT | 2 cut(s) | 517, 981 |
| TaqI | TCGA | 4 cut(s) | 532, 958, 1006, 1062 |
| TatI | WGTACW | 1 cut(s) | 1110 |
| TfiI | GAWTC | 5 cut(s) | 107, 113, 140, 244, 904 |
| Tru1I | TTAA | 4 cut(s) | 219, 315, 462, 840 |
| Tru9I | TTAA | 4 cut(s) | 219, 315, 462, 840 |
| TscAI | CASTG | 3 cut(s) | 789, 897, 1167 |
| TseFI | GTSAC | 2 cut(s) | 223, 974 |
| TseI | GCWGC | 3 cut(s) | 727, 1265, 1340 |
| Tsp45I | GTSAC | 2 cut(s) | 223, 974 |
| TspDTI | ATGAA | 4 cut(s) | 339, 1148, 1192, 1302 |
| TspGWI | ACGGA | 2 cut(s) | 438, 1071 |
| TspRI | CASTG | 3 cut(s) | 789, 897, 1167 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1175 |
| XapI | RAATTY | 2 cut(s) | 215, 360 |
| XceI | RCATGY | 1 cut(s) | 1121 |
| XmiI | GTMKAC | 1 cut(s) | 452 |
| XmnI | GAANNNNTTC | 1 cut(s) | 954 |
| XspI | CTAG | 5 cut(s) | 284, 293, 356, 395, 1143 |