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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 2 cut(s) | 622, 903 |
| AccB1I | GGYRCC | 3 cut(s) | 622, 903, 1060 |
| AccI | GTMKAC | 2 cut(s) | 1122, 1210 |
| AccIII | TCCGGA | 1 cut(s) | 265 |
| AclWI | GGATC | 3 cut(s) | 953, 966, 1278 |
| AcoI | YGGCCR | 1 cut(s) | 171 |
| AcsI | RAATTY | 1 cut(s) | 801 |
| AcuI | CTGAAG | 2 cut(s) | 105, 817 |
| AfaI | GTAC | 3 cut(s) | 624, 905, 1020 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 228, 913 |
| AgsI | TTSAA | 5 cut(s) | 413, 550, 705, 771, 996 |
| AjnI | CCWGG | 1 cut(s) | 634 |
| AluBI | AGCT | 2 cut(s) | 164, 217 |
| AluI | AGCT | 2 cut(s) | 164, 217 |
| Alw21I | GWGCWC | 1 cut(s) | 219 |
| Alw26I | GTCTC | 2 cut(s) | 389, 979 |
| AlwI | GGATC | 3 cut(s) | 953, 966, 1278 |
| Ama87I | CYCGRG | 2 cut(s) | 226, 816 |
| Aor13HI | TCCGGA | 1 cut(s) | 265 |
| AoxI | GGCC | 2 cut(s) | 75, 171 |
| ApeKI | GCWGC | 1 cut(s) | 458 |
| ApoI | RAATTY | 1 cut(s) | 801 |
| Asp718I | GGTACC | 2 cut(s) | 622, 903 |
| AspLEI | GCGC | 2 cut(s) | 256, 878 |
| AspS9I | GGNCC | 2 cut(s) | 712, 916 |
| AsuII | TTCGAA | 1 cut(s) | 356 |
| AvaI | CYCGRG | 2 cut(s) | 226, 816 |
| AvaII | GGWCC | 2 cut(s) | 712, 916 |
| BalI | TGGCCA | 1 cut(s) | 173 |
| BamHI | GGATCC | 1 cut(s) | 958 |
| BanI | GGYRCC | 3 cut(s) | 622, 903, 1060 |
| BanII | GRGCYC | 1 cut(s) | 219 |
| Bbv12I | GWGCWC | 1 cut(s) | 219 |
| BbvI | GCAGC | 1 cut(s) | 445 |
| BccI | CCATC | 8 cut(s) | 20, 64, 230, 333, 692, 775, 1156, 1227 |
| BciT130I | CCWGG | 1 cut(s) | 636 |
| BcoDI | GTCTC | 2 cut(s) | 389, 979 |
| BfaI | CTAG | 3 cut(s) | 333, 717, 791 |
| BfoI | RGCGCY | 1 cut(s) | 257 |
| BisI | GCNGC | 1 cut(s) | 459 |
| BlpI | GCTNAGC | 1 cut(s) | 872 |
| BlsI | GCNGC | 1 cut(s) | 460 |
| Bme1390I | CCNGG | 1 cut(s) | 636 |
| Bme18I | GGWCC | 2 cut(s) | 712, 916 |
| BmeT110I | CYCGRG | 2 cut(s) | 226, 816 |
| BmgT120I | GGNCC | 2 cut(s) | 712, 916 |
| BmiI | GGNNCC | 5 cut(s) | 624, 714, 905, 960, 1062 |
| BmrFI | CCNGG | 1 cut(s) | 636 |
| BmrI | ACTGGG | 1 cut(s) | 1068 |
| BmsI | GCATC | 3 cut(s) | 385, 550, 1017 |
| BmuI | ACTGGG | 1 cut(s) | 1068 |
| BpmI | CTGGAG | 1 cut(s) | 392 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 872 |
| Bpu14I | TTCGAA | 1 cut(s) | 356 |
| BpuEI | CTTGAG | 1 cut(s) | 941 |
| BsaJI | CCNNGG | 1 cut(s) | 756 |
| BsaWI | WCCGGW | 3 cut(s) | 166, 265, 670 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 65, 95 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 228, 913 |
| Bse1I | ACTGG | 1 cut(s) | 1074 |
| Bse3DI | GCAATG | 2 cut(s) | 490, 872 |
| BseAI | TCCGGA | 1 cut(s) | 265 |
| BseBI | CCWGG | 1 cut(s) | 636 |
| BseDI | CCNNGG | 1 cut(s) | 756 |
| BseGI | GGATG | 3 cut(s) | 1032, 1092, 1167 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 228, 913 |
| BseMI | GCAATG | 2 cut(s) | 490, 872 |
| BseMII | CTCAG | 1 cut(s) | 886 |
| BseNI | ACTGG | 1 cut(s) | 1074 |
| BseRI | GAGGAG | 2 cut(s) | 170, 407 |
| BseXI | GCAGC | 1 cut(s) | 445 |
| BsgI | GTGCAG | 1 cut(s) | 948 |
| BshFI | GGCC | 2 cut(s) | 77, 173 |
| BshNI | GGYRCC | 3 cut(s) | 622, 903, 1060 |
| BsiHKAI | GWGCWC | 1 cut(s) | 219 |
| BsiHKCI | CYCGRG | 2 cut(s) | 226, 816 |
| BsiSI | CCGG | 3 cut(s) | 167, 266, 671 |
| BslFI | GGGAC | 1 cut(s) | 698 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 228, 913 |
| BsmAI | GTCTC | 2 cut(s) | 389, 979 |
| BsmBI | CGTCTC | 1 cut(s) | 979 |
| BsmFI | GGGAC | 1 cut(s) | 698 |
| BsnI | GGCC | 2 cut(s) | 77, 173 |
| BsoBI | CYCGRG | 2 cut(s) | 226, 816 |
| Bsp119I | TTCGAA | 1 cut(s) | 356 |
| Bsp1286I | GDGCHC | 1 cut(s) | 219 |
| Bsp13I | TCCGGA | 1 cut(s) | 265 |
| Bsp143I | GATC | 4 cut(s) | 100, 958, 1045, 1283 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 872 |
| Bsp19I | CCATGG | 1 cut(s) | 756 |
| BspANI | GGCC | 2 cut(s) | 77, 173 |
| BspCNI | CTCAG | 1 cut(s) | 885 |
| BspEI | TCCGGA | 1 cut(s) | 265 |
| BspHI | TCATGA | 1 cut(s) | 687 |
| BspLI | GGNNCC | 5 cut(s) | 624, 714, 905, 960, 1062 |
| BspPI | GGATC | 3 cut(s) | 953, 966, 1278 |
| BspT104I | TTCGAA | 1 cut(s) | 356 |
| BspT107I | GGYRCC | 3 cut(s) | 622, 903, 1060 |
| BsrDI | GCAATG | 2 cut(s) | 490, 872 |
| BsrI | ACTGG | 1 cut(s) | 1074 |
| BssECI | CCNNGG | 1 cut(s) | 756 |
| BssMI | GATC | 4 cut(s) | 100, 958, 1045, 1283 |
| BssT1I | CCWWGG | 1 cut(s) | 756 |
| Bst2UI | CCWGG | 1 cut(s) | 636 |
| Bst4CI | ACNGT | 3 cut(s) | 36, 735, 1171 |
| Bst6I | CTCTTC | 1 cut(s) | 510 |
| BstBI | TTCGAA | 1 cut(s) | 356 |
| BstC8I | GCNNGC | 3 cut(s) | 215, 219, 435 |
| BstDEI | CTNAG | 2 cut(s) | 198, 872 |
| BstDSI | CCRYGG | 1 cut(s) | 756 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 911 |
| BstF5I | GGATG | 3 cut(s) | 1032, 1092, 1167 |
| BstH2I | RGCGCY | 1 cut(s) | 257 |
| BstHHI | GCGC | 2 cut(s) | 256, 878 |
| BstKTI | GATC | 4 cut(s) | 103, 961, 1048, 1286 |
| BstMAI | GTCTC | 2 cut(s) | 389, 979 |
| BstMBI | GATC | 4 cut(s) | 100, 958, 1045, 1283 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 170, 467, 882 |
| BstNI | CCWGG | 1 cut(s) | 636 |
| BstSCI | CCNGG | 1 cut(s) | 634 |
| BstV1I | GCAGC | 1 cut(s) | 445 |
| BstX2I | RGATCY | 2 cut(s) | 958, 1283 |
| BstYI | RGATCY | 2 cut(s) | 958, 1283 |
| BsuRI | GGCC | 2 cut(s) | 77, 173 |
| BtgI | CCRYGG | 1 cut(s) | 756 |
| BtsCI | GGATG | 3 cut(s) | 1032, 1092, 1167 |
| BtsIMutI | CAGTG | 3 cut(s) | 731, 776, 1081 |
| Cac8I | GCNNGC | 3 cut(s) | 215, 219, 435 |
| CciI | TCATGA | 1 cut(s) | 687 |
| CfoI | GCGC | 2 cut(s) | 256, 878 |
| Cfr13I | GGNCC | 2 cut(s) | 712, 916 |
| Csp6I | GTAC | 3 cut(s) | 623, 904, 1019 |
| CviAII | CATG | 9 cut(s) | 79, 140, 688, 695, 757, 898, 934, 1034, 1214 |
| CviQI | GTAC | 3 cut(s) | 623, 904, 1019 |
| DdeI | CTNAG | 2 cut(s) | 198, 872 |
| DpnI | GATC | 4 cut(s) | 102, 960, 1047, 1285 |
| DpnII | GATC | 4 cut(s) | 100, 958, 1045, 1283 |
| DraI | TTTAAA | 1 cut(s) | 567 |
| EaeI | YGGCCR | 1 cut(s) | 171 |
| Eam1104I | CTCTTC | 1 cut(s) | 510 |
| EarI | CTCTTC | 1 cut(s) | 510 |
| Ecl136II | GAGCTC | 1 cut(s) | 217 |
| Eco130I | CCWWGG | 1 cut(s) | 756 |
| Eco24I | GRGCYC | 1 cut(s) | 219 |
| Eco47I | GGWCC | 2 cut(s) | 712, 916 |
| Eco53kI | GAGCTC | 1 cut(s) | 217 |
| Eco57I | CTGAAG | 2 cut(s) | 105, 817 |
| Eco88I | CYCGRG | 2 cut(s) | 226, 816 |
| EcoICRI | GAGCTC | 1 cut(s) | 217 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 911 |
| EcoO109I | RGGNCCY | 1 cut(s) | 916 |
| EcoRII | CCWGG | 1 cut(s) | 634 |
| EcoT14I | CCWWGG | 1 cut(s) | 756 |
| EcoT22I | ATGCAT | 1 cut(s) | 1301 |
| EcoT38I | GRGCYC | 1 cut(s) | 219 |
| ErhI | CCWWGG | 1 cut(s) | 756 |
| Esp3I | CGTCTC | 1 cut(s) | 979 |
| FaeI | CATG | 9 cut(s) | 82, 143, 691, 698, 760, 901, 937, 1037, 1217 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 81, 113 |
| FaqI | GGGAC | 1 cut(s) | 698 |
| FatI | CATG | 9 cut(s) | 78, 139, 687, 694, 756, 897, 933, 1033, 1213 |
| FblI | GTMKAC | 2 cut(s) | 1122, 1210 |
| Fnu4HI | GCNGC | 1 cut(s) | 459 |
| FokI | GGATG | 3 cut(s) | 1039, 1099, 1174 |
| FriOI | GRGCYC | 1 cut(s) | 219 |
| Fsp4HI | GCNGC | 1 cut(s) | 459 |
| FspBI | CTAG | 3 cut(s) | 333, 717, 791 |
| GlaI | GCGC | 2 cut(s) | 255, 877 |
| GluI | GCNGC | 1 cut(s) | 459 |
| GsuI | CTGGAG | 1 cut(s) | 392 |
| HaeII | RGCGCY | 1 cut(s) | 257 |
| HaeIII | GGCC | 2 cut(s) | 77, 173 |
| HapII | CCGG | 3 cut(s) | 167, 266, 671 |
| HhaI | GCGC | 2 cut(s) | 256, 878 |
| Hin1II | CATG | 9 cut(s) | 82, 143, 691, 698, 760, 901, 937, 1037, 1217 |
| Hin6I | GCGC | 2 cut(s) | 254, 876 |
| HinP1I | GCGC | 2 cut(s) | 254, 876 |
| HincII | GTYRAC | 2 cut(s) | 307, 1092 |
| HindII | GTYRAC | 2 cut(s) | 307, 1092 |
| HinfI | GANTC | 3 cut(s) | 8, 992, 1133 |
| HpaI | GTTAAC | 1 cut(s) | 307 |
| HpaII | CCGG | 3 cut(s) | 167, 266, 671 |
| Hpy166II | GTNNAC | 9 cut(s) | 137, 307, 1009, 1092, 1123, 1132, 1174, 1211, 1309 |
| Hpy188I | TCNGA | 6 cut(s) | 85, 809, 836, 931, 991, 1045 |
| Hpy188III | TCNNGA | 7 cut(s) | 16, 48, 266, 371, 688, 791, 818 |
| Hpy8I | GTNNAC | 9 cut(s) | 137, 307, 1009, 1092, 1123, 1132, 1174, 1211, 1309 |
| HpyAV | CCTTC | 5 cut(s) | 125, 321, 336, 352, 510 |
| HpyCH4III | ACNGT | 3 cut(s) | 36, 735, 1171 |
| HpyCH4IV | ACGT | 1 cut(s) | 421 |
| HpyCH4V | TGCA | 8 cut(s) | 461, 746, 865, 885, 965, 1030, 1181, 1299 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 170, 467, 882 |
| HpyF3I | CTNAG | 2 cut(s) | 198, 872 |
| HpySE526I | ACGT | 1 cut(s) | 421 |
| Hsp92II | CATG | 9 cut(s) | 82, 143, 691, 698, 760, 901, 937, 1037, 1217 |
| HspAI | GCGC | 2 cut(s) | 254, 876 |
| Kpn2I | TCCGGA | 1 cut(s) | 265 |
| KpnI | GGTACC | 2 cut(s) | 626, 907 |
| KspAI | GTTAAC | 1 cut(s) | 307 |
| Kzo9I | GATC | 4 cut(s) | 100, 958, 1045, 1283 |
| LmnI | GCTCC | 3 cut(s) | 169, 373, 673 |
| LpnPI | CCDG | 9 cut(s) | 180, 261, 279, 356, 621, 648, 684, 975, 1087 |
| Lsp1109I | GCAGC | 1 cut(s) | 445 |
| LweI | GCATC | 3 cut(s) | 385, 550, 1017 |
| MaeI | CTAG | 3 cut(s) | 333, 717, 791 |
| MaeII | ACGT | 1 cut(s) | 421 |
| MaeIII | GTNAC | 1 cut(s) | 1076 |
| MalI | GATC | 4 cut(s) | 102, 960, 1047, 1285 |
| MboI | GATC | 4 cut(s) | 100, 958, 1045, 1283 |
| MboII | GAAGA | 8 cut(s) | 98, 101, 104, 130, 320, 497, 823, 1118 |
| MflI | RGATCY | 2 cut(s) | 958, 1283 |
| MhlI | GDGCHC | 1 cut(s) | 219 |
| MlsI | TGGCCA | 1 cut(s) | 173 |
| MluCI | AATT | 8 cut(s) | 187, 597, 741, 766, 801, 1012, 1187, 1200 |
| MluNI | TGGCCA | 1 cut(s) | 173 |
| MlyI | GAGTC | 1 cut(s) | 1127 |
| Mox20I | TGGCCA | 1 cut(s) | 173 |
| Mph1103I | ATGCAT | 1 cut(s) | 1301 |
| MroI | TCCGGA | 1 cut(s) | 265 |
| MscI | TGGCCA | 1 cut(s) | 173 |
| MseI | TTAA | 4 cut(s) | 306, 566, 854, 1194 |
| MslI | CAYNNNNRTG | 2 cut(s) | 686, 1231 |
| Msp20I | TGGCCA | 1 cut(s) | 173 |
| MspI | CCGG | 3 cut(s) | 167, 266, 671 |
| MspR9I | CCNGG | 1 cut(s) | 636 |
| MvaI | CCWGG | 1 cut(s) | 636 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 170, 467, 882 |
| NcoI | CCATGG | 1 cut(s) | 756 |
| NdeII | GATC | 4 cut(s) | 100, 958, 1045, 1283 |
| NlaIII | CATG | 9 cut(s) | 82, 143, 691, 698, 760, 901, 937, 1037, 1217 |
| NlaIV | GGNNCC | 5 cut(s) | 624, 714, 905, 960, 1062 |
| NmuCI | GTSAC | 1 cut(s) | 1076 |
| NsiI | ATGCAT | 1 cut(s) | 1301 |
| NspV | TTCGAA | 1 cut(s) | 356 |
| PaeR7I | CTCGAG | 1 cut(s) | 816 |
| PagI | TCATGA | 1 cut(s) | 687 |
| PfeI | GAWTC | 2 cut(s) | 8, 992 |
| PflFI | GACNNNGTC | 1 cut(s) | 1118 |
| PkrI | GCNGC | 1 cut(s) | 460 |
| PleI | GAGTC | 1 cut(s) | 1127 |
| PpsI | GAGTC | 1 cut(s) | 1127 |
| PpuMI | RGGWCCY | 1 cut(s) | 916 |
| Psp124BI | GAGCTC | 1 cut(s) | 219 |
| Psp5II | RGGWCCY | 1 cut(s) | 916 |
| Psp6I | CCWGG | 1 cut(s) | 634 |
| PspGI | CCWGG | 1 cut(s) | 634 |
| PspN4I | GGNNCC | 5 cut(s) | 624, 714, 905, 960, 1062 |
| PspPI | GGNCC | 2 cut(s) | 712, 916 |
| PspPPI | RGGWCCY | 1 cut(s) | 916 |
| PsuI | RGATCY | 2 cut(s) | 958, 1283 |
| PsyI | GACNNNGTC | 1 cut(s) | 1118 |
| RsaI | GTAC | 3 cut(s) | 624, 905, 1020 |
| RsaNI | GTAC | 3 cut(s) | 623, 904, 1019 |
| RseI | CAYNNNNRTG | 2 cut(s) | 686, 1231 |
| SacI | GAGCTC | 1 cut(s) | 219 |
| SaqAI | TTAA | 4 cut(s) | 306, 566, 854, 1194 |
| SatI | GCNGC | 1 cut(s) | 459 |
| Sau3AI | GATC | 4 cut(s) | 100, 958, 1045, 1283 |
| Sau96I | GGNCC | 2 cut(s) | 712, 916 |
| SchI | GAGTC | 1 cut(s) | 1127 |
| ScrFI | CCNGG | 1 cut(s) | 636 |
| SduI | GDGCHC | 1 cut(s) | 219 |
| SfaNI | GCATC | 3 cut(s) | 385, 550, 1017 |
| Sfr274I | CTCGAG | 1 cut(s) | 816 |
| SfuI | TTCGAA | 1 cut(s) | 356 |
| SinI | GGWCC | 2 cut(s) | 712, 916 |
| SlaI | CTCGAG | 1 cut(s) | 816 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 686, 1231 |
| SmlI | CTYRAG | 2 cut(s) | 816, 920 |
| SmoI | CTYRAG | 2 cut(s) | 816, 920 |
| Sse9I | AATT | 8 cut(s) | 187, 597, 741, 766, 801, 1012, 1187, 1200 |
| SspMI | CTAG | 3 cut(s) | 333, 717, 791 |
| SstI | GAGCTC | 1 cut(s) | 219 |
| StyD4I | CCNGG | 1 cut(s) | 634 |
| StyI | CCWWGG | 1 cut(s) | 756 |
| TaaI | ACNGT | 3 cut(s) | 36, 735, 1171 |
| TaiI | ACGT | 1 cut(s) | 424 |
| TaqI | TCGA | 4 cut(s) | 15, 356, 618, 817 |
| TasI | AATT | 8 cut(s) | 187, 597, 741, 766, 801, 1012, 1187, 1200 |
| TatI | WGTACW | 1 cut(s) | 1018 |
| TfiI | GAWTC | 2 cut(s) | 8, 992 |
| Tru1I | TTAA | 4 cut(s) | 306, 566, 854, 1194 |
| Tru9I | TTAA | 4 cut(s) | 306, 566, 854, 1194 |
| TscAI | CASTG | 3 cut(s) | 738, 783, 1081 |
| TseFI | GTSAC | 1 cut(s) | 1076 |
| TseI | GCWGC | 1 cut(s) | 458 |
| Tsp45I | GTSAC | 1 cut(s) | 1076 |
| TspDTI | ATGAA | 5 cut(s) | 39, 659, 676, 704, 1119 |
| TspGWI | ACGGA | 1 cut(s) | 1039 |
| TspRI | CASTG | 3 cut(s) | 738, 783, 1081 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1118 |
| VpaK11BI | GGWCC | 2 cut(s) | 712, 916 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 911 |
| XapI | RAATTY | 1 cut(s) | 801 |
| XbaI | TCTAGA | 1 cut(s) | 790 |
| XhoI | CTCGAG | 1 cut(s) | 816 |
| XmiI | GTMKAC | 2 cut(s) | 1122, 1210 |
| XspI | CTAG | 3 cut(s) | 333, 717, 791 |
| Zsp2I | ATGCAT | 1 cut(s) | 1301 |