Rh5DG259700

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000290
Physical Location & Seq
Reverse (-)
30980356 .. 30983293
2938 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG259700.1

Sequence Viewer

Length: 1179 bp
ATGGAGTTTTGCTCCGAGTTTGTTCAACGTTGTAATCCAATTGGAGTTCCTAAGGATCAAAGTAAGCTCTGTGGCCCACTTTCTACTGCGAAACTTAAGTGCATTGATGAGAAGGAGAGAGATCAGGCACATGTTCATGTGTTGTCCAATAATGATGAGGTGGATCCATATAAGATTGCACATAAAAACATTGTAGAGCAACTGTATGCTGGGAAGAAGAGGAGTAAACAATGGCTCCTTAGTGAGCACAATCGTACTTTTGCTGATTGGTTTGAAGCCAAGTTGGAAGCAATGGGTTCGAAGAAGAAGGAAAAGGCTGAAAGTGATAAAAAGTCAAAGAAACATTTTGATTTGGAGACTTGTTCTGAGGCTGACAAGGTGGCTGATAAGAAAGCAGAATCTGAATCTGCAGAGACCATCACAAGCACCGAATCAACTAAAAGAAGAGGGAAGCGAAGTGTGGTGGAAATGTATAAGGTTGTGATCAAGAAAGCTTTGGGGAAAAAATTTAAAGTGACATACACCGACACGGGGAATCCCAATGGACGAGTACGACACACTTTGCAATCATATATAGGGATGCTCGCGCGGAAAATGGTGCCCATTAACATTGTAAGCTGGCCCGAAGTAGACGGTGAATTGAAAGATAAAATTTGGATTGACGTCCAGGATACATTCAAAGTGGCCCCTGAAAGCAAGAAATTGGTGTTGACATCTGCTGGCACTAAATGGAGACAATTCAAGACCAACTTAACAAATAAGTATGTGCTGCCATACTTAGGAAAGAAGAAGAAATTGAGAAAGCCACCAAAGCAGTATGCATTCCTTGGGTTGTTGCCATGGAAGGAGTTTGTAAGTCAGAGGACTACAGAGGCATGGCTGAAACTTCATAATGACCAAAGTAAACGAGTCAAGAAGAGAAAATACCATCATAGATTATCAAGAAAGGGATATATTGGACTAGAGGAGGAATTGGTTATTGACCGTGCAATTCTATGGAAGAAGGCCCGTGTACTTAAAAATGGGGAGCTAAGTGAGGAAGTGACACCGATTGCAACAAAGATAGATGAATTGATGGAGAAGAAAAGGAAGGGTGAGCTAGAAATTTCAGGGAGTAGTGATGTATTATCTCAAGCACTAGAAACTCCTGAACATTCGGGCAGAATTACTGGCACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 Transposase_24

Protein Analysis

392

Amino Acids

45.03

Weight (kDa)

9.64

Isoelectric Point (pI)

35.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000290)

Species Orthologous Gene IDs
prunus_persica Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1 Prupe.7G026800_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0352291 RchiOBHm_Chr1g0352301 RchiOBHm_Chr2g0145261 RchiOBHm_Chr2g0160781 RchiOBHm_Chr2g0160871 RchiOBHm_Chr3g0460901 RchiOBHm_Chr3g0494431 RchiOBHm_Chr3g0494441 RchiOBHm_Chr4g0385451 RchiOBHm_Chr4g0417051 RchiOBHm_Chr4g0424111 RchiOBHm_Chr5g0035911 RchiOBHm_Chr6g0269251 RchiOBHm_Chr6g0274141 RchiOBHm_Chr7g0191841 RchiOBHm_Chr7g0196691 RchiOBHm_Chr7g0211271 RchiOBHm_Chr7g0227321 RchiOBHm_Chr7g0242811
rosa_laevigata RLG00000013212
rosa_multiflora Rmu_co8387591.1_g000001 Rmu_sc0000147.1_g000094 Rmu_sc0000340.1_g000034 Rmu_sc0000546.1_g000048 Rmu_sc0001461.1_g000092 Rmu_sc0001659.1_g000042 Rmu_sc0002460.1_g000027 Rmu_sc0003170.1_g000021 Rmu_sc0004301.1_g000015 Rmu_sc0004344.1_g000009 Rmu_sc0005539.1_g000024 Rmu_sc0006803.1_g000018 Rmu_sc0006888.1_g000057 Rmu_sc0009330.1_g000004 Rmu_sc0010704.1_g000001 Rmu_sc0012787.1_g000002 Rmu_sc0023684.1_g000002 Rmu_ssc0000189.1_g000010
rosa_roxburghii Rroxscaffold_5G00377480 Rroxscaffold_6G00389290 Rroxscaffold_6G00414410 Rroxscaffold_7G00202010
rosa_rugosa Rorug01G0242100 Rorug02G0188500 Rorug02G0273900 Rorug03G0342600 Rorug05G0239000 Rorug07G0163300 Rorug07G0192100
rosa_samantha Rh1BG091000 Rh1BG200300 Rh1DG228100 Rh2AG268900 Rh2AG431600 Rh2AG508400 Rh2CG258700 Rh2CG417500 Rh2CG493900 Rh3AG323800 Rh3BG237800 Rh3CG306700 Rh3DG204700 Rh3DG259400 Rh4AG203100 Rh4AG377200 Rh4BG054300 Rh4BG125100 Rh4BG146000 Rh4BG251900 Rh4DG132500 Rh4DG155000 Rh4DG384000 Rh5BG230700 Rh5CG382700 Rh5CG532300 Rh5DG235600 Rh5DG259700 Rh5DG320000 Rh6AG162900 Rh6CG065400 Rh6CG065500 Rh6CG307600 Rh6CG310500 Rh6CG391900 Rh6DG062500 Rh6DG062600 Rh6DG187500 Rh7BG296200 Rh7DG266900 Rh7DG322300
rosa_wichuraiana Rw2G018510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 666
AccB1I GGYRCC 1 cut(s) 598
AccI GTMKAC 1 cut(s) 630
AccII CGCG 2 cut(s) 587, 589
AciI CCGC 1 cut(s) 589
AclI AACGTT 1 cut(s) 28
AclWI GGATC 3 cut(s) 63, 158, 171
AcsI RAATTY 3 cut(s) 506, 651, 1104
AcvI CACGTG 1 cut(s) 1176
AcyI GRCGYC 1 cut(s) 663
AfaI GTAC 3 cut(s) 256, 552, 1014
AfiI CCNNNNNNNGG 2 cut(s) 531, 779
AflII CTTAAG 1 cut(s) 95
AflIII ACRYGT 1 cut(s) 130
AgsI TTSAA 5 cut(s) 26, 275, 643, 679, 742
AjnI CCWGG 1 cut(s) 666
AluBI AGCT 5 cut(s) 67, 494, 618, 1030, 1099
AluI AGCT 5 cut(s) 67, 494, 618, 1030, 1099
Alw21I GWGCWC 1 cut(s) 249
Alw26I GTCTC 3 cut(s) 350, 407, 727
AlwI GGATC 3 cut(s) 63, 158, 171
AlwNI CAGNNNCTG 1 cut(s) 401
AoxI GGCC 4 cut(s) 73, 620, 684, 1005
ApeKI GCWGC 1 cut(s) 769
ApoI RAATTY 3 cut(s) 506, 651, 1104
AspLEI GCGC 1 cut(s) 589
AspS9I GGNCC 4 cut(s) 74, 621, 685, 1006
AsuHPI GGTGA 2 cut(s) 647, 1106
AsuII TTCGAA 1 cut(s) 299
AxyI CCTNAGG 1 cut(s) 51
BaeGI GKGCMC 1 cut(s) 603
BamHI GGATCC 1 cut(s) 163
BanI GGYRCC 1 cut(s) 598
BarI GAAGNNNNNNTAC 2 cut(s) 908, 940
BbrPI CACGTG 1 cut(s) 1176
Bbv12I GWGCWC 1 cut(s) 249
BbvI GCAGC 1 cut(s) 756
BccI CCATC 3 cut(s) 425, 936, 1069
BcgI CGANNNNNNTGC 2 cut(s) 279, 313
BciT130I CCWGG 1 cut(s) 668
BciVI GTATCC 1 cut(s) 664
BclI TGATCA 1 cut(s) 483
BcoDI GTCTC 3 cut(s) 350, 407, 727
BfaI CTAG 3 cut(s) 962, 1100, 1139
BfmI CTRYAG 2 cut(s) 408, 867
BfrI CTTAAG 1 cut(s) 95
BfuI GTATCC 1 cut(s) 664
BisI GCNGC 1 cut(s) 770
BlsI GCNGC 1 cut(s) 771
Bme1390I CCNGG 1 cut(s) 668
BmgT120I GGNCC 4 cut(s) 74, 621, 685, 1006
BmiI GGNNCC 4 cut(s) 165, 236, 600, 687
BmrFI CCNGG 1 cut(s) 668
BmsI GCATC 1 cut(s) 570
BplI GAGNNNNNCTC 1 cut(s) 28
Bpu14I TTCGAA 1 cut(s) 299
BpuEI CTTGAG 1 cut(s) 1116
BsaAI YACGTR 1 cut(s) 1176
BsaHI GRCGYC 1 cut(s) 663
BsaI GGTCTC 1 cut(s) 407
BsaJI CCNNGG 2 cut(s) 826, 839
BsaXI ACNNNNNCTCC 2 cut(s) 219, 249
Bsc4I CCNNNNNNNGG 2 cut(s) 531, 779
Bse1I ACTGG 1 cut(s) 1174
Bse21I CCTNAGG 1 cut(s) 51
Bse3DI GCAATG 1 cut(s) 297
BseBI CCWGG 1 cut(s) 668
BseDI CCNNGG 2 cut(s) 826, 839
BseGI GGATG 1 cut(s) 585
BseLI CCNNNNNNNGG 2 cut(s) 531, 779
BseMI GCAATG 1 cut(s) 297
BseMII CTCAG 1 cut(s) 357
BseNI ACTGG 1 cut(s) 1174
BseRI GAGGAG 2 cut(s) 235, 980
BseSI GKGCMC 1 cut(s) 603
BseXI GCAGC 1 cut(s) 756
BseYI CCCAGC 1 cut(s) 209
Bsh1236I CGCG 2 cut(s) 587, 589
BshFI GGCC 4 cut(s) 75, 622, 686, 1007
BshNI GGYRCC 1 cut(s) 598
BsiHKAI GWGCWC 1 cut(s) 249
BslI CCNNNNNNNGG 2 cut(s) 531, 779
BsmAI GTCTC 3 cut(s) 350, 407, 727
BsmI GAATGC 1 cut(s) 821
BsnI GGCC 4 cut(s) 75, 622, 686, 1007
Bso31I GGTCTC 1 cut(s) 407
Bsp119I TTCGAA 1 cut(s) 299
Bsp1286I GDGCHC 2 cut(s) 249, 603
Bsp143I GATC 4 cut(s) 55, 121, 163, 483
Bsp19I CCATGG 1 cut(s) 839
BspACI CCGC 1 cut(s) 589
BspANI GGCC 4 cut(s) 75, 622, 686, 1007
BspCNI CTCAG 1 cut(s) 358
BspFNI CGCG 2 cut(s) 587, 589
BspLI GGNNCC 4 cut(s) 165, 236, 600, 687
BspMAI CTGCAG 1 cut(s) 412
BspPI GGATC 3 cut(s) 63, 158, 171
BspT104I TTCGAA 1 cut(s) 299
BspT107I GGYRCC 1 cut(s) 598
BspTI CTTAAG 1 cut(s) 95
BspTNI GGTCTC 1 cut(s) 407
BsrDI GCAATG 1 cut(s) 297
BsrI ACTGG 1 cut(s) 1174
BssECI CCNNGG 2 cut(s) 826, 839
BssMI GATC 4 cut(s) 55, 121, 163, 483
BssNI GRCGYC 1 cut(s) 663
BssT1I CCWWGG 2 cut(s) 826, 839
Bst2UI CCWGG 1 cut(s) 668
Bst4CI ACNGT 3 cut(s) 204, 635, 986
Bst6I CTCTTC 3 cut(s) 212, 439, 911
BstACI GRCGYC 1 cut(s) 663
BstAFI CTTAAG 1 cut(s) 95
BstBAI YACGTR 1 cut(s) 1176
BstBI TTCGAA 1 cut(s) 299
BstC8I GCNNGC 3 cut(s) 585, 620, 721
BstDEI CTNAG 5 cut(s) 51, 239, 366, 778, 1031
BstDSI CCRYGG 1 cut(s) 839
BstF5I GGATG 1 cut(s) 585
BstFNI CGCG 2 cut(s) 587, 589
BstHHI GCGC 1 cut(s) 589
BstKTI GATC 4 cut(s) 58, 124, 166, 486
BstMAI GTCTC 3 cut(s) 350, 407, 727
BstMBI GATC 4 cut(s) 55, 121, 163, 483
BstMWI GCNNNNNNNGC 1 cut(s) 811
BstNI CCWGG 1 cut(s) 668
BstNSI RCATGY 1 cut(s) 134
BstSCI CCNGG 1 cut(s) 666
BstSFI CTRYAG 2 cut(s) 408, 867
BstSLI GKGCMC 1 cut(s) 603
BstUI CGCG 2 cut(s) 587, 589
BstV1I GCAGC 1 cut(s) 756
BstX2I RGATCY 1 cut(s) 163
BstYI RGATCY 1 cut(s) 163
Bsu36I CCTNAGG 1 cut(s) 51
BsuI GTATCC 1 cut(s) 664
BsuRI GGCC 4 cut(s) 75, 622, 686, 1007
BtgI CCRYGG 1 cut(s) 839
BtsCI GGATG 1 cut(s) 585
Cac8I GCNNGC 3 cut(s) 585, 620, 721
CaiI CAGNNNCTG 1 cut(s) 401
CfoI GCGC 1 cut(s) 589
Cfr13I GGNCC 4 cut(s) 74, 621, 685, 1006
Csp6I GTAC 3 cut(s) 255, 551, 1013
CviAII CATG 4 cut(s) 131, 137, 840, 876
CviQI GTAC 3 cut(s) 255, 551, 1013
DdeI CTNAG 5 cut(s) 51, 239, 366, 778, 1031
DpnI GATC 4 cut(s) 57, 123, 165, 485
DpnII GATC 4 cut(s) 55, 121, 163, 483
DraI TTTAAA 1 cut(s) 511
Eam1104I CTCTTC 3 cut(s) 212, 439, 911
EarI CTCTTC 3 cut(s) 212, 439, 911
Eco130I CCWWGG 2 cut(s) 826, 839
Eco31I GGTCTC 1 cut(s) 407
Eco72I CACGTG 1 cut(s) 1176
Eco81I CCTNAGG 1 cut(s) 51
EcoRII CCWGG 1 cut(s) 666
EcoT14I CCWWGG 2 cut(s) 826, 839
EcoT22I ATGCAT 1 cut(s) 823
ErhI CCWWGG 2 cut(s) 826, 839
FaeI CATG 4 cut(s) 134, 140, 843, 879
FalI AAGNNNNNCTT 2 cut(s) 734, 766
FatI CATG 4 cut(s) 130, 136, 839, 875
FbaI TGATCA 1 cut(s) 483
FblI GTMKAC 1 cut(s) 630
Fnu4HI GCNGC 1 cut(s) 770
FokI GGATG 1 cut(s) 592
Fsp4HI GCNGC 1 cut(s) 770
FspBI CTAG 3 cut(s) 962, 1100, 1139
GlaI GCGC 1 cut(s) 588
GluI GCNGC 1 cut(s) 770
GsaI CCCAGC 1 cut(s) 213
HaeIII GGCC 4 cut(s) 75, 622, 686, 1007
HhaI GCGC 1 cut(s) 589
Hin1I GRCGYC 1 cut(s) 663
Hin1II CATG 4 cut(s) 134, 140, 843, 879
Hin6I GCGC 1 cut(s) 587
HinP1I GCGC 1 cut(s) 587
HincII GTYRAC 1 cut(s) 711
HindII GTYRAC 1 cut(s) 711
HindIII AAGCTT 1 cut(s) 492
HinfI GANTC 5 cut(s) 398, 404, 431, 535, 909
HphI GGTGA 2 cut(s) 647, 1106
Hpy166II GTNNAC 5 cut(s) 227, 631, 711, 905, 1013
Hpy188I TCNGA 4 cut(s) 16, 367, 403, 861
Hpy188III TCNNGA 5 cut(s) 487, 742, 913, 942, 1148
Hpy8I GTNNAC 5 cut(s) 227, 631, 711, 905, 1013
HpyAV CCTTC 5 cut(s) 106, 301, 838, 997, 1084
HpyCH4III ACNGT 3 cut(s) 204, 635, 986
HpyCH4IV ACGT 3 cut(s) 28, 663, 1175
HpyCH4V TGCA 7 cut(s) 102, 179, 410, 565, 821, 989, 1055
HpyF10VI GCNNNNNNNGC 1 cut(s) 811
HpyF3I CTNAG 5 cut(s) 51, 239, 366, 778, 1031
HpySE526I ACGT 3 cut(s) 28, 663, 1175
Hsp92I GRCGYC 1 cut(s) 663
Hsp92II CATG 4 cut(s) 134, 140, 843, 879
HspAI GCGC 1 cut(s) 587
Ksp22I TGATCA 1 cut(s) 483
Kzo9I GATC 4 cut(s) 55, 121, 163, 483
LmnI GCTCC 3 cut(s) 17, 240, 1027
Lsp1109I GCAGC 1 cut(s) 756
LweI GCATC 1 cut(s) 570
MaeI CTAG 3 cut(s) 962, 1100, 1139
MaeII ACGT 3 cut(s) 28, 663, 1175
MaeIII GTNAC 2 cut(s) 514, 1042
MalI GATC 4 cut(s) 57, 123, 165, 485
MboI GATC 4 cut(s) 55, 121, 163, 483
MfeI CAATTG 1 cut(s) 39
MflI RGATCY 1 cut(s) 163
MhlI GDGCHC 2 cut(s) 249, 603
MlyI GAGTC 1 cut(s) 918
MmeI TCCRAC 1 cut(s) 264
MnlI CCTC 9 cut(s) 151, 213, 361, 440, 855, 865, 958, 961, 1030
Mph1103I ATGCAT 1 cut(s) 823
MseI TTAA 5 cut(s) 96, 510, 606, 752, 1017
MslI CAYNNNNRTG 1 cut(s) 135
MspCI CTTAAG 1 cut(s) 95
MspR9I CCNGG 1 cut(s) 668
MunI CAATTG 1 cut(s) 39
Mva1269I GAATGC 1 cut(s) 821
MvaI CCWGG 1 cut(s) 668
MvnI CGCG 2 cut(s) 587, 589
MwoI GCNNNNNNNGC 1 cut(s) 811
NcoI CCATGG 1 cut(s) 839
NdeII GATC 4 cut(s) 55, 121, 163, 483
NlaIII CATG 4 cut(s) 134, 140, 843, 879
NlaIV GGNNCC 4 cut(s) 165, 236, 600, 687
NmuCI GTSAC 2 cut(s) 514, 1042
NsiI ATGCAT 1 cut(s) 823
NspI RCATGY 1 cut(s) 134
NspV TTCGAA 1 cut(s) 299
PciI ACATGT 1 cut(s) 130
PctI GAATGC 1 cut(s) 821
PfeI GAWTC 4 cut(s) 398, 404, 431, 535
PfoI TCCNGGA 1 cut(s) 666
PkrI GCNGC 1 cut(s) 771
PleI GAGTC 1 cut(s) 917
PmaCI CACGTG 1 cut(s) 1176
PmlI CACGTG 1 cut(s) 1176
PpsI GAGTC 1 cut(s) 917
Ppu21I YACGTR 1 cut(s) 1176
PscI ACATGT 1 cut(s) 130
Psp1406I AACGTT 1 cut(s) 28
Psp6I CCWGG 1 cut(s) 666
PspCI CACGTG 1 cut(s) 1176
PspFI CCCAGC 1 cut(s) 209
PspGI CCWGG 1 cut(s) 666
PspN4I GGNNCC 4 cut(s) 165, 236, 600, 687
PspPI GGNCC 4 cut(s) 74, 621, 685, 1006
PstI CTGCAG 1 cut(s) 412
PstNI CAGNNNCTG 1 cut(s) 401
PsuI RGATCY 1 cut(s) 163
RsaI GTAC 3 cut(s) 256, 552, 1014
RsaNI GTAC 3 cut(s) 255, 551, 1013
RseI CAYNNNNRTG 1 cut(s) 135
SaqAI TTAA 5 cut(s) 96, 510, 606, 752, 1017
SatI GCNGC 1 cut(s) 770
Sau3AI GATC 4 cut(s) 55, 121, 163, 483
Sau96I GGNCC 4 cut(s) 74, 621, 685, 1006
SchI GAGTC 1 cut(s) 918
ScrFI CCNGG 1 cut(s) 668
SduI GDGCHC 2 cut(s) 249, 603
SfaNI GCATC 1 cut(s) 570
SfcI CTRYAG 2 cut(s) 408, 867
SfuI TTCGAA 1 cut(s) 299
SmiMI CAYNNNNRTG 1 cut(s) 135
SmlI CTYRAG 2 cut(s) 95, 1131
SmoI CTYRAG 2 cut(s) 95, 1131
SsiI CCGC 1 cut(s) 589
SspMI CTAG 3 cut(s) 962, 1100, 1139
StyD4I CCNGG 1 cut(s) 666
StyI CCWWGG 2 cut(s) 826, 839
TaaI ACNGT 3 cut(s) 204, 635, 986
TaiI ACGT 3 cut(s) 31, 666, 1178
TaqI TCGA 1 cut(s) 299
TatI WGTACW 1 cut(s) 1012
TfiI GAWTC 4 cut(s) 398, 404, 431, 535
Tru1I TTAA 5 cut(s) 96, 510, 606, 752, 1017
Tru9I TTAA 5 cut(s) 96, 510, 606, 752, 1017
TseFI GTSAC 2 cut(s) 514, 1042
TseI GCWGC 1 cut(s) 769
Tsp45I GTSAC 2 cut(s) 514, 1042
TspDTI ATGAA 3 cut(s) 125, 878, 1083
Vha464I CTTAAG 1 cut(s) 95
XapI RAATTY 3 cut(s) 506, 651, 1104
XceI RCATGY 1 cut(s) 134
XmiI GTMKAC 1 cut(s) 630
XspI CTAG 3 cut(s) 962, 1100, 1139
ZraI GACGTC 1 cut(s) 664
Zsp2I ATGCAT 1 cut(s) 823
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.