Rmu_sc0000974.1_g000024

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000974.1
OG0000419
Physical Location & Seq
Forward (+)
104104 .. 107040
2937 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000974.1_g000024.1.cds

Sequence Viewer

Length: 1392 bp
atggcaatgttcagccaaagagaagacgacgaaggacgtaatcccagaagcagaaggactgagaaagaacaggaacctgaagaggatcctaaagaaaccaaagaaaaaattcttaaaaggtttctgctaagtccaaagggcttggcctcaactgatcaatcccagggtaaaggattggctagcccgtctcaaacggcagacggtaaaggcacatcaagaccgttgatggctgctgatttgccaaaaagcagaccaactccaagtagagttataaatgtttttaattatgaattaagaacttttgatactcctgtgttcagaactggcaggagactagcttatcaccagaaggcaatcctggataatctcttatttgcctttcaagaaagaagcagtggtctgatgttcccagctctctttgctttagctgaagaactctacgagaatggtagaccacaatttgaagacagtcatatacagcagagtgctgtaagaaaagaaaaaattatcttccttgaaagtccagaaagtgctagagttcctgtcatggcacttaatgaatcagactggcatgaattccatagtctgataggaagacataatccagaagacctattcagatccactcatccagaatggatccaaaaggcaaatggtgaagttgaatatattcccccataccattatgtacgaatcattcaaaggagatatcttcaaccagcctgtgtagggtacaatggccaaccaaactcaagcatcccgtggatgaaggccatgactctagaatacatcaaaaagatcatatctgaagacatccacaataccttcctggcagcaggagagaaaatcatcatcactacttacgatcatcctgacgtagtcagcagtgacttattcctatctctcaccagtaaagagatagactcgacactggcatgcttacgatgggtggaaaaacttgaaacagacaaccactacaagatgtatgttgatacatatgtcgaagaaaatacaacaactgctcgaatggcccaggcaaataacgactcgtttgtccttgtcgttgtggtgaggccgaggacgaggttggctgtgaaaaagctgaaggaggaggaggtgctgccggagctggagaatgagaaggagaaggaccgcgtgattgtgatttcggagaaggaagactccaattcggagggtgaaagggaggaagaagaaggagagaacaaagctgtgatggctgacgatagtggtggcttgagtgctaacaaggttgctgggcaagaagaagagaactgtacgacgccgtttccagaaaaggtatccaaaaatttgcgcctttatttcagttgggtcgatgctggttctgatgaattttgttccttatttgtttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04392 map04011 map04392
Pfam Domains
Protein Families
Pkinase

Protein Analysis

463

Amino Acids

52.98

Weight (kDa)

5.21

Isoelectric Point (pI)

62.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000419)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0378961 RchiOBHm_Chr3g0452751 RchiOBHm_Chr6g0244511 RchiOBHm_Chr6g0265751 RchiOBHm_Chr7g0243311
rosa_laevigata RLG00000002693 RLG00000003022 RLG00000006790 RLG00000008553
rosa_multiflora Rmu_sc0000063.1_g000020 Rmu_sc0000239.1_g000008 Rmu_sc0000365.1_g000035 Rmu_sc0000431.1_g000029 Rmu_sc0000528.1_g000019 Rmu_sc0000536.1_g000014 Rmu_sc0000663.1_g000004 Rmu_sc0000753.1_g000003 Rmu_sc0000754.1_g000014 Rmu_sc0000805.1_g000036 Rmu_sc0000974.1_g000024 Rmu_sc0000976.1_g000012 Rmu_sc0001097.1_g000047 Rmu_sc0001122.1_g000010 Rmu_sc0001207.1_g000032 Rmu_sc0001214.1_g000001 Rmu_sc0001323.1_g000017 Rmu_sc0001673.1_g000006 Rmu_sc0001785.1_g000018 Rmu_sc0001959.1_g000012 Rmu_sc0002073.1_g000006 Rmu_sc0002076.1_g000005 Rmu_sc0002094.1_g000004 Rmu_sc0002132.1_g000057 Rmu_sc0002170.1_g000036 Rmu_sc0002187.1_g000010 Rmu_sc0002316.1_g000037 Rmu_sc0002324.1_g000009 Rmu_sc0002367.1_g000001 Rmu_sc0002482.1_g000001 Rmu_sc0002547.1_g000017 Rmu_sc0002578.1_g000012 Rmu_sc0002693.1_g000016 Rmu_sc0002706.1_g000004 Rmu_sc0002759.1_g000032 Rmu_sc0002765.1_g000002 Rmu_sc0002845.1_g000026 Rmu_sc0002895.1_g000014 Rmu_sc0002983.1_g000013 Rmu_sc0003187.1_g000017 Rmu_sc0003249.1_g000029 Rmu_sc0003342.1_g000038 Rmu_sc0003374.1_g000002 Rmu_sc0003470.1_g000017 Rmu_sc0003669.1_g000013 Rmu_sc0003693.1_g000008 Rmu_sc0003749.1_g000015 Rmu_sc0003833.1_g000005 Rmu_sc0003936.1_g000036 Rmu_sc0004798.1_g000001 Rmu_sc0004932.1_g000024 Rmu_sc0005088.1_g000004 Rmu_sc0005439.1_g000003 Rmu_sc0005532.1_g000002 Rmu_sc0005728.1_g000009 Rmu_sc0005879.1_g000012 Rmu_sc0005914.1_g000010 Rmu_sc0006112.1_g000002 Rmu_sc0006196.1_g000003 Rmu_sc0006248.1_g000001 Rmu_sc0006366.1_g000015 Rmu_sc0006567.1_g000015 Rmu_sc0006695.1_g000070 Rmu_sc0006937.1_g000004 Rmu_sc0007647.1_g000009 Rmu_sc0008681.1_g000001 Rmu_sc0009833.1_g000019 Rmu_sc0010256.1_g000006 Rmu_sc0010366.1_g000001 Rmu_sc0010462.1_g000006 Rmu_sc0010817.1_g000017 Rmu_sc0014139.1_g000002 Rmu_sc0015191.1_g000001 Rmu_sc0015649.1_g000002 Rmu_sc0017650.1_g000005 Rmu_sc0028733.1_g000002 Rmu_ssc0000024.1_g000019 Rmu_ssc0000134.1_g000059 Rmu_ssc0000400.1_g000040 Rmu_ssc0000438.1_g000049
rosa_roxburghii Rroxscaffold_3G00231160 Rroxscaffold_5G00354700 Rroxscaffold_6G00395540
rosa_rugosa Rorug01G0149700.1 Rorug03G0299500 Rorug07G0249000
rosa_samantha Rh3BG164500 Rh3BG164800 Rh6BG099500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 272
AasI GACNNNNNNGTC 1 cut(s) 1052
AccI GTMKAC 1 cut(s) 451
AccII CGCG 1 cut(s) 1155
AciI CCGC 1 cut(s) 1153
AclWI GGATC 5 cut(s) 80, 93, 615, 634, 647
AcoI YGGCCR 1 cut(s) 739
AcsI RAATTY 4 cut(s) 108, 575, 1327, 1370
AcuI CTGAAG 4 cut(s) 99, 450, 828, 1124
AcyI GRCGYC 1 cut(s) 1301
AfaI GTAC 3 cut(s) 690, 734, 1297
AfiI CCNNNNNNNGG 1 cut(s) 729
AgsI TTSAA 7 cut(s) 383, 464, 518, 665, 701, 716, 962
AjnI CCWGG 4 cut(s) 162, 357, 828, 1032
AluBI AGCT 6 cut(s) 338, 413, 428, 1102, 1129, 1229
AluI AGCT 6 cut(s) 338, 413, 428, 1102, 1129, 1229
Alw26I GTCTC 2 cut(s) 192, 325
AlwI GGATC 5 cut(s) 80, 93, 615, 634, 647
AoxI GGCC 5 cut(s) 144, 739, 771, 1029, 1073
ApeKI GCWGC 3 cut(s) 230, 833, 1120
ApoI RAATTY 4 cut(s) 108, 575, 1327, 1370
Asp700I GAANNNNTTC 2 cut(s) 108, 669
AspLEI GCGC 1 cut(s) 1335
AspS9I GGNCC 2 cut(s) 1030, 1150
AsuHPI GGTGA 5 cut(s) 335, 668, 898, 1081, 1208
AsuNHI GCTAGC 1 cut(s) 179
AvaII GGWCC 1 cut(s) 1150
BaeI ACNNNNGTAYC 4 cut(s) 297, 330, 984, 1017
BalI TGGCCA 1 cut(s) 741
BamHI GGATCC 2 cut(s) 85, 639
BbsI GAAGAC 6 cut(s) 30, 471, 601, 615, 816, 1185
BbvI GCAGC 3 cut(s) 217, 845, 1107
BccI CCATC 3 cut(s) 220, 939, 1228
BceAI ACGGC 2 cut(s) 210, 1288
BciT130I CCWGG 4 cut(s) 164, 359, 830, 1034
BciVI GTATCC 1 cut(s) 1330
BclI TGATCA 1 cut(s) 154
BcoDI GTCTC 2 cut(s) 192, 325
BfaI CTAG 4 cut(s) 180, 335, 534, 782
BfuI GTATCC 1 cut(s) 1330
BisI GCNGC 3 cut(s) 231, 834, 1121
BlsI GCNGC 3 cut(s) 232, 835, 1122
Bme1390I CCNGG 4 cut(s) 164, 359, 830, 1034
Bme18I GGWCC 1 cut(s) 1150
BmgT120I GGNCC 2 cut(s) 1030, 1150
BmiI GGNNCC 3 cut(s) 75, 87, 641
BmrFI CCNGG 4 cut(s) 164, 359, 830, 1034
BmsI GCATC 2 cut(s) 765, 1345
BmtI GCTAGC 1 cut(s) 183
BpiI GAAGAC 6 cut(s) 30, 471, 601, 615, 816, 1185
BplI GAGNNNNNCTC 2 cut(s) 908, 940
BpmI CTGGAG 1 cut(s) 1151
BpuEI CTTGAG 2 cut(s) 736, 1276
BsaHI GRCGYC 1 cut(s) 1301
BsaJI CCNNGG 5 cut(s) 162, 163, 761, 1032, 1076
Bsc4I CCNNNNNNNGG 1 cut(s) 729
Bse1I ACTGG 4 cut(s) 328, 572, 909, 936
Bse3DI GCAATG 1 cut(s) 12
BseBI CCWGG 4 cut(s) 164, 359, 830, 1034
BseDI CCNNGG 5 cut(s) 162, 163, 761, 1032, 1076
BseGI GGATG 5 cut(s) 628, 756, 771, 813, 868
BseLI CCNNNNNNNGG 1 cut(s) 729
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 1 cut(s) 51
BseNI ACTGG 4 cut(s) 328, 572, 909, 936
BseRI GAGGAG 2 cut(s) 1124, 1127
BseXI GCAGC 3 cut(s) 217, 845, 1107
BseYI CCCAGC 2 cut(s) 409, 1274
Bsh1236I CGCG 1 cut(s) 1155
BshFI GGCC 5 cut(s) 146, 741, 773, 1031, 1075
BsiSI CCGG 1 cut(s) 1124
BslI CCNNNNNNNGG 1 cut(s) 729
BsmAI GTCTC 2 cut(s) 192, 325
BsmBI CGTCTC 1 cut(s) 192
BsnI GGCC 5 cut(s) 146, 741, 773, 1031, 1075
Bsp143I GATC 6 cut(s) 85, 154, 620, 639, 798, 865
BspACI CCGC 1 cut(s) 1153
BspANI GGCC 5 cut(s) 146, 741, 773, 1031, 1075
BspCNI CTCAG 1 cut(s) 52
BspFNI CGCG 1 cut(s) 1155
BspLI GGNNCC 3 cut(s) 75, 87, 641
BspOI GCTAGC 1 cut(s) 183
BspPI GGATC 5 cut(s) 80, 93, 615, 634, 647
BsrDI GCAATG 1 cut(s) 12
BsrI ACTGG 4 cut(s) 328, 572, 909, 936
BssECI CCNNGG 5 cut(s) 162, 163, 761, 1032, 1076
BssMI GATC 6 cut(s) 85, 154, 620, 639, 798, 865
BssNI GRCGYC 1 cut(s) 1301
Bst2UI CCWGG 4 cut(s) 164, 359, 830, 1034
Bst4CI ACNGT 4 cut(s) 203, 222, 470, 1295
Bst6I CTCTTC 2 cut(s) 75, 1281
BstACI GRCGYC 1 cut(s) 1301
BstC8I GCNNGC 2 cut(s) 181, 937
BstDEI CTNAG 2 cut(s) 60, 128
BstDSI CCRYGG 1 cut(s) 761
BstF5I GGATG 5 cut(s) 628, 756, 771, 813, 868
BstFNI CGCG 1 cut(s) 1155
BstHHI GCGC 1 cut(s) 1335
BstKTI GATC 6 cut(s) 88, 157, 623, 642, 801, 868
BstMAI GTCTC 2 cut(s) 192, 325
BstMBI GATC 6 cut(s) 85, 154, 620, 639, 798, 865
BstMWI GCNNNNNNNGC 4 cut(s) 419, 1028, 1126, 1235
BstNI CCWGG 4 cut(s) 164, 359, 830, 1034
BstNSI RCATGY 1 cut(s) 939
BstSCI CCNGG 4 cut(s) 162, 357, 828, 1032
BstUI CGCG 1 cut(s) 1155
BstV1I GCAGC 3 cut(s) 217, 845, 1107
BstV2I GAAGAC 6 cut(s) 30, 471, 601, 615, 816, 1185
BstX2I RGATCY 3 cut(s) 85, 620, 639
BstYI RGATCY 3 cut(s) 85, 620, 639
BsuI GTATCC 1 cut(s) 1330
BsuRI GGCC 5 cut(s) 146, 741, 773, 1031, 1075
BtgI CCRYGG 1 cut(s) 761
BtsCI GGATG 5 cut(s) 628, 756, 771, 813, 868
BtsI GCAGTG 2 cut(s) 400, 892
BtsIMutI CAGTG 3 cut(s) 400, 892, 929
Cac8I GCNNGC 2 cut(s) 181, 937
CfoI GCGC 1 cut(s) 1335
Cfr13I GGNCC 2 cut(s) 1030, 1150
CseI GACGC 1 cut(s) 1309
Csp6I GTAC 3 cut(s) 689, 733, 1296
CviAII CATG 4 cut(s) 547, 572, 775, 936
CviQI GTAC 3 cut(s) 689, 733, 1296
DdeI CTNAG 2 cut(s) 60, 128
DpnI GATC 6 cut(s) 87, 156, 622, 641, 800, 867
DpnII GATC 6 cut(s) 85, 154, 620, 639, 798, 865
DrdI GACNNNNNNGTC 1 cut(s) 1052
DseDI GACNNNNNNGTC 1 cut(s) 1052
EaeI YGGCCR 1 cut(s) 739
Eam1104I CTCTTC 2 cut(s) 75, 1281
EarI CTCTTC 2 cut(s) 75, 1281
Eco32I GATATC 1 cut(s) 710
Eco47I GGWCC 1 cut(s) 1150
Eco57I CTGAAG 4 cut(s) 99, 450, 828, 1124
EcoRI GAATTC 1 cut(s) 575
EcoRII CCWGG 4 cut(s) 162, 357, 828, 1032
EcoRV GATATC 1 cut(s) 710
Esp3I CGTCTC 1 cut(s) 192
FaeI CATG 4 cut(s) 550, 575, 778, 939
FatI CATG 4 cut(s) 546, 571, 774, 935
FauNDI CATATG 1 cut(s) 997
FbaI TGATCA 1 cut(s) 154
FblI GTMKAC 1 cut(s) 451
Fnu4HI GCNGC 3 cut(s) 231, 834, 1121
FokI GGATG 5 cut(s) 615, 743, 778, 800, 855
Fsp4HI GCNGC 3 cut(s) 231, 834, 1121
FspBI CTAG 4 cut(s) 180, 335, 534, 782
GlaI GCGC 1 cut(s) 1334
GluI GCNGC 3 cut(s) 231, 834, 1121
GsaI CCCAGC 2 cut(s) 413, 1278
GsuI CTGGAG 1 cut(s) 1151
HaeIII GGCC 5 cut(s) 146, 741, 773, 1031, 1075
HapII CCGG 1 cut(s) 1124
HgaI GACGC 1 cut(s) 1309
HhaI GCGC 1 cut(s) 1335
Hin1I GRCGYC 1 cut(s) 1301
Hin1II CATG 4 cut(s) 550, 575, 778, 939
Hin6I GCGC 1 cut(s) 1333
HinP1I GCGC 1 cut(s) 1333
HinfI GANTC 6 cut(s) 560, 693, 778, 923, 1046, 1181
HpaII CCGG 1 cut(s) 1124
HphI GGTGA 5 cut(s) 335, 668, 898, 1081, 1208
Hpy166II GTNNAC 1 cut(s) 452
Hpy188I TCNGA 9 cut(s) 320, 402, 565, 588, 620, 808, 1171, 1192, 1366
Hpy188III TCNNGA 8 cut(s) 216, 383, 524, 605, 632, 782, 872, 1310
Hpy8I GTNNAC 1 cut(s) 452
Hpy99I CGWCG 2 cut(s) 32, 1303
HpyCH4III ACNGT 4 cut(s) 203, 222, 470, 1295
HpyCH4IV ACGT 2 cut(s) 37, 876
HpyF10VI GCNNNNNNNGC 4 cut(s) 419, 1028, 1126, 1235
HpyF3I CTNAG 2 cut(s) 60, 128
HpySE526I ACGT 2 cut(s) 37, 876
Hsp92I GRCGYC 1 cut(s) 1301
Hsp92II CATG 4 cut(s) 550, 575, 778, 939
HspAI GCGC 1 cut(s) 1333
Ksp22I TGATCA 1 cut(s) 154
Kzo9I GATC 6 cut(s) 85, 154, 620, 639, 798, 865
LmnI GCTCC 1 cut(s) 1126
Lsp1109I GCAGC 3 cut(s) 217, 845, 1107
LweI GCATC 2 cut(s) 765, 1345
MaeI CTAG 4 cut(s) 180, 335, 534, 782
MaeII ACGT 2 cut(s) 37, 876
MaeIII GTNAC 1 cut(s) 887
MalI GATC 6 cut(s) 87, 156, 622, 641, 800, 867
MboI GATC 6 cut(s) 85, 154, 620, 639, 798, 865
MflI RGATCY 3 cut(s) 85, 620, 639
MlsI TGGCCA 1 cut(s) 741
MluCI AATT 9 cut(s) 108, 283, 290, 458, 504, 575, 1186, 1327, 1370
MluNI TGGCCA 1 cut(s) 741
MlyI GAGTC 4 cut(s) 772, 917, 1040, 1175
Mox20I TGGCCA 1 cut(s) 741
MroXI GAANNNNTTC 2 cut(s) 108, 669
MscI TGGCCA 1 cut(s) 741
MseI TTAA 5 cut(s) 114, 282, 293, 555, 1390
MslI CAYNNNNRTG 1 cut(s) 934
Msp20I TGGCCA 1 cut(s) 741
MspI CCGG 1 cut(s) 1124
MspR9I CCNGG 4 cut(s) 164, 359, 830, 1034
MvaI CCWGG 4 cut(s) 164, 359, 830, 1034
MvnI CGCG 1 cut(s) 1155
MwoI GCNNNNNNNGC 4 cut(s) 419, 1028, 1126, 1235
NdeI CATATG 1 cut(s) 997
NdeII GATC 6 cut(s) 85, 154, 620, 639, 798, 865
NheI GCTAGC 1 cut(s) 179
NlaIII CATG 4 cut(s) 550, 575, 778, 939
NlaIV GGNNCC 3 cut(s) 75, 87, 641
NmeAIII GCCGAG 1 cut(s) 1101
NmuCI GTSAC 1 cut(s) 887
NspI RCATGY 1 cut(s) 939
PaeI GCATGC 1 cut(s) 939
PasI CCCWGGG 1 cut(s) 163
PdmI GAANNNNTTC 2 cut(s) 108, 669
PfeI GAWTC 2 cut(s) 560, 693
PflFI GACNNNGTC 1 cut(s) 878
PfoI TCCNGGA 1 cut(s) 357
PkrI GCNGC 3 cut(s) 232, 835, 1122
PleI GAGTC 4 cut(s) 772, 917, 1040, 1175
PpsI GAGTC 4 cut(s) 772, 917, 1040, 1175
PsiI TTATAA 1 cut(s) 272
Psp6I CCWGG 4 cut(s) 162, 357, 828, 1032
PspFI CCCAGC 2 cut(s) 409, 1274
PspGI CCWGG 4 cut(s) 162, 357, 828, 1032
PspN4I GGNNCC 3 cut(s) 75, 87, 641
PspPI GGNCC 2 cut(s) 1030, 1150
PsrI GAACNNNNNNTAC 2 cut(s) 289, 321
PsuI RGATCY 3 cut(s) 85, 620, 639
PsyI GACNNNGTC 1 cut(s) 878
RsaI GTAC 3 cut(s) 690, 734, 1297
RsaNI GTAC 3 cut(s) 689, 733, 1296
RseI CAYNNNNRTG 1 cut(s) 934
SaqAI TTAA 5 cut(s) 114, 282, 293, 555, 1390
SatI GCNGC 3 cut(s) 231, 834, 1121
Sau3AI GATC 6 cut(s) 85, 154, 620, 639, 798, 865
Sau96I GGNCC 2 cut(s) 1030, 1150
SchI GAGTC 4 cut(s) 772, 917, 1040, 1175
ScrFI CCNGG 4 cut(s) 164, 359, 830, 1034
SfaNI GCATC 2 cut(s) 765, 1345
SinI GGWCC 1 cut(s) 1150
SmiMI CAYNNNNRTG 1 cut(s) 934
SmlI CTYRAG 2 cut(s) 751, 1255
SmoI CTYRAG 2 cut(s) 751, 1255
SphI GCATGC 1 cut(s) 939
Sse9I AATT 9 cut(s) 108, 283, 290, 458, 504, 575, 1186, 1327, 1370
SsiI CCGC 1 cut(s) 1153
SspMI CTAG 4 cut(s) 180, 335, 534, 782
StyD4I CCNGG 4 cut(s) 162, 357, 828, 1032
TaaI ACNGT 4 cut(s) 203, 222, 470, 1295
TaiI ACGT 2 cut(s) 40, 879
TaqI TCGA 4 cut(s) 926, 1002, 1024, 1353
TasI AATT 9 cut(s) 108, 283, 290, 458, 504, 575, 1186, 1327, 1370
TfiI GAWTC 2 cut(s) 560, 693
Tru1I TTAA 5 cut(s) 114, 282, 293, 555, 1390
Tru9I TTAA 5 cut(s) 114, 282, 293, 555, 1390
TscAI CASTG 3 cut(s) 400, 892, 936
TseFI GTSAC 1 cut(s) 887
TseI GCWGC 3 cut(s) 230, 833, 1120
Tsp45I GTSAC 1 cut(s) 887
TspDTI ATGAA 5 cut(s) 303, 573, 588, 782, 1383
TspRI CASTG 3 cut(s) 400, 892, 936
Tth111I GACNNNGTC 1 cut(s) 878
VpaK11BI GGWCC 1 cut(s) 1150
XapI RAATTY 4 cut(s) 108, 575, 1327, 1370
XbaI TCTAGA 1 cut(s) 781
XceI RCATGY 1 cut(s) 939
XcmI CCANNNNNNNNNTGG 1 cut(s) 650
XmiI GTMKAC 1 cut(s) 451
XmnI GAANNNNTTC 2 cut(s) 108, 669
XspI CTAG 4 cut(s) 180, 335, 534, 782
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.