Rroxscaffold_5G00354700

Enzymatic polyprotein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000419
Physical Location & Seq
Forward (+)
33201126 .. 33208985
7860 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00354700.1

Sequence Viewer

Length: 771 bp
ATGTTATCTCCTAAGACAAGTTCCAAAAGAAAATGCAACTTCACAGAAGATGATGAAAAAATAGTGAAGCAGAAAAAAAATCTCTGCAAAAACCTCCCTCCTCTTCAACAACCAGAAGAGAATGATGAAATTATCCTCCGGACGTATGCGAGTGATAATTATTGGTCCGGAGTGGTTCTGGCAAAAACGCTTGGAACTAACATTGAAAAAATCTGTAAATTTTGTAGTGGCAAATTCAGCCCTGCAGAACTAAATTATCCCACAGGGGAAAAAGAAATTTTGGCTGTCAAGAAAACAATTTTAAATTCTCCAGCTTTTCTTGGAAAACCCTTTACCGTCCATACCGATTGTGCTAGAGTAAAGAATTTCAAAAATTTTAAATTTGATAAAGCTACCGATAGAGGAAGATTAGCAAACTGGCAATTATTTCTTAACCAATATGATTATGTTGTTGAATTAATTGCAGGAAATAAGAACTATCTTCCGTACGCCTTAACCGAGAACCGGCAATTTTTAGCCGAAAAGATGTCGACGAAGGTCACAATCACGAAGCGAAGAACCGGGAAAGAACATGAACTCGAAGAGGATCCCATGGAAACCATAGAAAAAATCCTCAAAAGGTTTATGCTAAGTCCAAAAGGACTAGCCCCAACCGATCAATCCCGGGGTAAAGGATTGGCTAGCCCGTCTCAAACGGCGGACGGTAAAGGCTCATCAAGACCGTTGAAGGCTGTTGCTTTGCCAAAAGCAAACCTACTCCAACCGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_A3 RVT_1

Protein Analysis

256

Amino Acids

28.9

Weight (kDa)

9.43

Isoelectric Point (pI)

53.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RT_RNaseH_2 PF17919 14 - 113 4.8e-14 RNase H-like domain found in reverse transcriptase
RT_RNaseH PF17917 41 - 147 8.5e-18 RNase H-like domain found in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000419)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0378961 RchiOBHm_Chr3g0452751 RchiOBHm_Chr6g0244511 RchiOBHm_Chr6g0265751 RchiOBHm_Chr7g0243311
rosa_laevigata RLG00000002693 RLG00000003022 RLG00000006790 RLG00000008553
rosa_multiflora Rmu_sc0000063.1_g000020 Rmu_sc0000239.1_g000008 Rmu_sc0000365.1_g000035 Rmu_sc0000431.1_g000029 Rmu_sc0000528.1_g000019 Rmu_sc0000536.1_g000014 Rmu_sc0000663.1_g000004 Rmu_sc0000753.1_g000003 Rmu_sc0000754.1_g000014 Rmu_sc0000805.1_g000036 Rmu_sc0000974.1_g000024 Rmu_sc0000976.1_g000012 Rmu_sc0001097.1_g000047 Rmu_sc0001122.1_g000010 Rmu_sc0001207.1_g000032 Rmu_sc0001214.1_g000001 Rmu_sc0001323.1_g000017 Rmu_sc0001673.1_g000006 Rmu_sc0001785.1_g000018 Rmu_sc0001959.1_g000012 Rmu_sc0002073.1_g000006 Rmu_sc0002076.1_g000005 Rmu_sc0002094.1_g000004 Rmu_sc0002132.1_g000057 Rmu_sc0002170.1_g000036 Rmu_sc0002187.1_g000010 Rmu_sc0002316.1_g000037 Rmu_sc0002324.1_g000009 Rmu_sc0002367.1_g000001 Rmu_sc0002482.1_g000001 Rmu_sc0002547.1_g000017 Rmu_sc0002578.1_g000012 Rmu_sc0002693.1_g000016 Rmu_sc0002706.1_g000004 Rmu_sc0002759.1_g000032 Rmu_sc0002765.1_g000002 Rmu_sc0002845.1_g000026 Rmu_sc0002895.1_g000014 Rmu_sc0002983.1_g000013 Rmu_sc0003187.1_g000017 Rmu_sc0003249.1_g000029 Rmu_sc0003342.1_g000038 Rmu_sc0003374.1_g000002 Rmu_sc0003470.1_g000017 Rmu_sc0003669.1_g000013 Rmu_sc0003693.1_g000008 Rmu_sc0003749.1_g000015 Rmu_sc0003833.1_g000005 Rmu_sc0003936.1_g000036 Rmu_sc0004798.1_g000001 Rmu_sc0004932.1_g000024 Rmu_sc0005088.1_g000004 Rmu_sc0005439.1_g000003 Rmu_sc0005532.1_g000002 Rmu_sc0005728.1_g000009 Rmu_sc0005879.1_g000012 Rmu_sc0005914.1_g000010 Rmu_sc0006112.1_g000002 Rmu_sc0006196.1_g000003 Rmu_sc0006248.1_g000001 Rmu_sc0006366.1_g000015 Rmu_sc0006567.1_g000015 Rmu_sc0006695.1_g000070 Rmu_sc0006937.1_g000004 Rmu_sc0007647.1_g000009 Rmu_sc0008681.1_g000001 Rmu_sc0009833.1_g000019 Rmu_sc0010256.1_g000006 Rmu_sc0010366.1_g000001 Rmu_sc0010462.1_g000006 Rmu_sc0010817.1_g000017 Rmu_sc0014139.1_g000002 Rmu_sc0015191.1_g000001 Rmu_sc0015649.1_g000002 Rmu_sc0017650.1_g000005 Rmu_sc0028733.1_g000002 Rmu_ssc0000024.1_g000019 Rmu_ssc0000134.1_g000059 Rmu_ssc0000400.1_g000040 Rmu_ssc0000438.1_g000049
rosa_roxburghii Rroxscaffold_3G00231160 Rroxscaffold_5G00354700 Rroxscaffold_6G00395540
rosa_rugosa Rorug01G0149700.1 Rorug03G0299500 Rorug07G0249000
rosa_samantha Rh3BG164500 Rh3BG164800 Rh6BG099500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 530
AccIII TCCGGA 2 cut(s) 138, 167
AciI CCGC 1 cut(s) 698
AclWI GGATC 2 cut(s) 581, 594
AcsI RAATTY 7 cut(s) 218, 233, 276, 304, 364, 373, 380
AfaI GTAC 1 cut(s) 488
AfiI CCNNNNNNNGG 1 cut(s) 504
AgsI TTSAA 5 cut(s) 107, 206, 370, 455, 727
AluBI AGCT 2 cut(s) 314, 392
AluI AGCT 2 cut(s) 314, 392
Alw26I GTCTC 1 cut(s) 693
AlwI GGATC 2 cut(s) 581, 594
Ama87I CYCGRG 1 cut(s) 663
Aor13HI TCCGGA 2 cut(s) 138, 167
ApoI RAATTY 7 cut(s) 218, 233, 276, 304, 364, 373, 380
AseI ATTAAT 1 cut(s) 458
AspS9I GGNCC 1 cut(s) 165
AsuC2I CCSGG 3 cut(s) 562, 664, 665
AsuNHI GCTAGC 1 cut(s) 680
AvaI CYCGRG 1 cut(s) 663
AvaII GGWCC 1 cut(s) 165
BamHI GGATCC 1 cut(s) 586
BceAI ACGGC 1 cut(s) 711
BcnI CCSGG 3 cut(s) 562, 664, 665
BcoDI GTCTC 1 cut(s) 693
BfaI CTAG 3 cut(s) 354, 644, 681
BfmI CTRYAG 1 cut(s) 243
Bme1390I CCNGG 3 cut(s) 562, 664, 665
Bme18I GGWCC 1 cut(s) 165
BmeT110I CYCGRG 1 cut(s) 663
BmgT120I GGNCC 1 cut(s) 165
BmiI GGNNCC 1 cut(s) 588
BmrFI CCNGG 3 cut(s) 562, 664, 665
BmtI GCTAGC 1 cut(s) 684
BoxI GACNNNNGTC 1 cut(s) 536
BpmI CTGGAG 1 cut(s) 294
BpuMI CCSGG 3 cut(s) 562, 664, 665
BsaJI CCNNGG 3 cut(s) 591, 663, 664
BsaWI WCCGGW 3 cut(s) 138, 167, 763
Bsc4I CCNNNNNNNGG 1 cut(s) 504
Bse118I RCCGGY 1 cut(s) 504
Bse1I ACTGG 1 cut(s) 422
BseAI TCCGGA 2 cut(s) 138, 167
BseDI CCNNGG 3 cut(s) 591, 663, 664
BseLI CCNNNNNNNGG 1 cut(s) 504
BseNI ACTGG 1 cut(s) 422
BseRI GAGGAG 1 cut(s) 90
BsiHKCI CYCGRG 1 cut(s) 663
BsiSI CCGG 6 cut(s) 139, 168, 505, 561, 664, 764
BsiWI CGTACG 1 cut(s) 486
BslI CCNNNNNNNGG 1 cut(s) 504
BsmAI GTCTC 1 cut(s) 693
BsmBI CGTCTC 1 cut(s) 693
BsoBI CYCGRG 1 cut(s) 663
Bsp13I TCCGGA 2 cut(s) 138, 167
Bsp143I GATC 2 cut(s) 586, 655
Bsp19I CCATGG 1 cut(s) 591
BspACI CCGC 1 cut(s) 698
BspEI TCCGGA 2 cut(s) 138, 167
BspLI GGNNCC 1 cut(s) 588
BspMAI CTGCAG 1 cut(s) 247
BspOI GCTAGC 1 cut(s) 684
BspPI GGATC 2 cut(s) 581, 594
BsrFI RCCGGY 1 cut(s) 504
BsrI ACTGG 1 cut(s) 422
BssAI RCCGGY 1 cut(s) 504
BssECI CCNNGG 3 cut(s) 591, 663, 664
BssMI GATC 2 cut(s) 586, 655
BssT1I CCWWGG 1 cut(s) 591
Bst4CI ACNGT 3 cut(s) 337, 704, 723
Bst6I CTCTTC 3 cut(s) 108, 111, 576
BstC8I GCNNGC 1 cut(s) 682
BstDEI CTNAG 2 cut(s) 12, 629
BstDSI CCRYGG 1 cut(s) 591
BstKTI GATC 2 cut(s) 589, 658
BstMAI GTCTC 1 cut(s) 693
BstMBI GATC 2 cut(s) 586, 655
BstMWI GCNNNNNNNGC 1 cut(s) 237
BstPAI GACNNNNGTC 1 cut(s) 536
BstSCI CCNGG 3 cut(s) 560, 662, 663
BstSFI CTRYAG 1 cut(s) 243
BstX2I RGATCY 1 cut(s) 586
BstYI RGATCY 1 cut(s) 586
BtgI CCRYGG 1 cut(s) 591
Cac8I GCNNGC 1 cut(s) 682
Cfr10I RCCGGY 1 cut(s) 504
Cfr13I GGNCC 1 cut(s) 165
Cfr9I CCCGGG 1 cut(s) 663
Csp6I GTAC 1 cut(s) 487
CviAII CATG 2 cut(s) 572, 592
CviQI GTAC 1 cut(s) 487
DdeI CTNAG 2 cut(s) 12, 629
DpnI GATC 2 cut(s) 588, 657
DpnII GATC 2 cut(s) 586, 655
DraI TTTAAA 2 cut(s) 303, 379
Eam1104I CTCTTC 3 cut(s) 108, 111, 576
EarI CTCTTC 3 cut(s) 108, 111, 576
EciI GGCGGA 1 cut(s) 713
Eco130I CCWWGG 1 cut(s) 591
Eco47I GGWCC 1 cut(s) 165
Eco88I CYCGRG 1 cut(s) 663
EcoT14I CCWWGG 1 cut(s) 591
ErhI CCWWGG 1 cut(s) 591
Esp3I CGTCTC 1 cut(s) 693
FaeI CATG 2 cut(s) 575, 595
FaiI YATR 8 cut(s) 147, 342, 441, 447, 573, 593, 602, 626
FatI CATG 2 cut(s) 571, 591
FblI GTMKAC 1 cut(s) 530
FspBI CTAG 3 cut(s) 354, 644, 681
GsuI CTGGAG 1 cut(s) 294
HapII CCGG 6 cut(s) 139, 168, 505, 561, 664, 764
Hin1II CATG 2 cut(s) 575, 595
HincII GTYRAC 1 cut(s) 531
HindII GTYRAC 1 cut(s) 531
HpaII CCGG 6 cut(s) 139, 168, 505, 561, 664, 764
Hpy166II GTNNAC 1 cut(s) 531
Hpy188III TCNNGA 5 cut(s) 139, 168, 289, 547, 717
Hpy8I GTNNAC 1 cut(s) 531
Hpy99I CGWCG 1 cut(s) 535
HpyAV CCTTC 2 cut(s) 529, 721
HpyCH4III ACNGT 3 cut(s) 337, 704, 723
HpyCH4IV ACGT 1 cut(s) 143
HpyCH4V TGCA 4 cut(s) 36, 87, 245, 464
HpyF10VI GCNNNNNNNGC 1 cut(s) 237
HpyF3I CTNAG 2 cut(s) 12, 629
HpySE526I ACGT 1 cut(s) 143
Hsp92II CATG 2 cut(s) 575, 595
Kpn2I TCCGGA 2 cut(s) 138, 167
Kzo9I GATC 2 cut(s) 586, 655
MaeI CTAG 3 cut(s) 354, 644, 681
MaeII ACGT 1 cut(s) 143
MaeIII GTNAC 1 cut(s) 538
MalI GATC 2 cut(s) 588, 657
MboI GATC 2 cut(s) 586, 655
MboII GAAGA 7 cut(s) 59, 95, 128, 417, 473, 567, 593
MflI RGATCY 1 cut(s) 586
MnlI CCTC 7 cut(s) 104, 108, 111, 146, 395, 577, 623
MroI TCCGGA 2 cut(s) 138, 167
MseI TTAA 5 cut(s) 302, 378, 432, 458, 494
MspI CCGG 6 cut(s) 139, 168, 505, 561, 664, 764
MspR9I CCNGG 3 cut(s) 562, 664, 665
MwoI GCNNNNNNNGC 1 cut(s) 237
NciI CCSGG 3 cut(s) 562, 664, 665
NcoI CCATGG 1 cut(s) 591
NdeII GATC 2 cut(s) 586, 655
NheI GCTAGC 1 cut(s) 680
NlaIII CATG 2 cut(s) 575, 595
NlaIV GGNNCC 1 cut(s) 588
NmuCI GTSAC 1 cut(s) 538
PcsI WCGNNNNNNNCGW 1 cut(s) 495
Pfl23II CGTACG 1 cut(s) 486
PshAI GACNNNNGTC 1 cut(s) 536
PshBI ATTAAT 1 cut(s) 458
PspLI CGTACG 1 cut(s) 486
PspN4I GGNNCC 1 cut(s) 588
PspPI GGNCC 1 cut(s) 165
PstI CTGCAG 1 cut(s) 247
PsuI RGATCY 1 cut(s) 586
RsaI GTAC 1 cut(s) 488
RsaNI GTAC 1 cut(s) 487
SalI GTCGAC 1 cut(s) 529
SaqAI TTAA 5 cut(s) 302, 378, 432, 458, 494
Sau3AI GATC 2 cut(s) 586, 655
Sau96I GGNCC 1 cut(s) 165
ScrFI CCNGG 3 cut(s) 562, 664, 665
SetI ASST 7 cut(s) 96, 146, 316, 394, 540, 623, 756
SfcI CTRYAG 1 cut(s) 243
SinI GGWCC 1 cut(s) 165
SmaI CCCGGG 1 cut(s) 665
SsiI CCGC 1 cut(s) 698
SspMI CTAG 3 cut(s) 354, 644, 681
StyD4I CCNGG 3 cut(s) 560, 662, 663
StyI CCWWGG 1 cut(s) 591
TaaI ACNGT 3 cut(s) 337, 704, 723
TaiI ACGT 1 cut(s) 146
TaqI TCGA 2 cut(s) 530, 579
Tru1I TTAA 5 cut(s) 302, 378, 432, 458, 494
Tru9I TTAA 5 cut(s) 302, 378, 432, 458, 494
TseFI GTSAC 1 cut(s) 538
Tsp45I GTSAC 1 cut(s) 538
TspDTI ATGAA 3 cut(s) 69, 141, 588
TspGWI ACGGA 1 cut(s) 474
TspMI CCCGGG 1 cut(s) 663
VpaK11BI GGWCC 1 cut(s) 165
VspI ATTAAT 1 cut(s) 458
XapI RAATTY 7 cut(s) 218, 233, 276, 304, 364, 373, 380
XmaI CCCGGG 1 cut(s) 663
XmiI GTMKAC 1 cut(s) 530
XspI CTAG 3 cut(s) 354, 644, 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.