Rmu_ssc0000024.1_g000019

Enzymatic polyprotein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000024.1
OG0000419
Physical Location & Seq
Forward (+)
85863 .. 87261
1399 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000024.1_g000019.1.cds

Sequence Viewer

Length: 912 bp
atgacgacgaaggttgtaatcccagaagcagaagaactgggaaagaacatgaacttgaagaggatcccaaagaaaaaaatcctcaaaaggtttctgctaagaccaaaaggattagccacaactgatcaatcccagggtaaaggattggttagccagtctcaaacggcagacggtaaaggctcatcaagaccgttgacggctgctgctttgccaaaaagcagaccaactccaactggagttataaatgtttttaattatgaattaagaacttttgatactcctgtgttcagaactggcaggagactagcttttcaccagaaggcaattttggataatcttttatttgcctttcaagaaagtagtagtggtctgatattcccagctctctttgcactagctaaagaactctatgaaaatgctaggccacagtttgaagaaattcatacacagcagagtgctatccagaaggcaaacggtgaagttgaatatattcctccatatcattatgtgagaattattcaaaggaaatatcttcaaccagcctgtgtagggtacaatggccaaccaaactcaagcatcccatggataaaggccatgactctagaatacatcaagaagatcatctctgaagatatccacaataccttcttggcagcaggagagaaaactattatcactgcttacgatcatcttgacgtaatcagcagtgacctattcctatctcttaccaaaaaggagatagattcgacactggcatgcttgcagtgggtggaaaggcttgaaactgacaaccactacaagatgtatgttgatacagatgtcgaagataatgctaccactgttagaatggcccaagcagacaacaactcctttgtctttggccacaattcagaaacatatgagaatatgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_A3 RVT_1

Protein Analysis

303

Amino Acids

34.28

Weight (kDa)

8.33

Isoelectric Point (pI)

48.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000419)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0378961 RchiOBHm_Chr3g0452751 RchiOBHm_Chr6g0244511 RchiOBHm_Chr6g0265751 RchiOBHm_Chr7g0243311
rosa_laevigata RLG00000002693 RLG00000003022 RLG00000006790 RLG00000008553
rosa_multiflora Rmu_sc0000063.1_g000020 Rmu_sc0000239.1_g000008 Rmu_sc0000365.1_g000035 Rmu_sc0000431.1_g000029 Rmu_sc0000528.1_g000019 Rmu_sc0000536.1_g000014 Rmu_sc0000663.1_g000004 Rmu_sc0000753.1_g000003 Rmu_sc0000754.1_g000014 Rmu_sc0000805.1_g000036 Rmu_sc0000974.1_g000024 Rmu_sc0000976.1_g000012 Rmu_sc0001097.1_g000047 Rmu_sc0001122.1_g000010 Rmu_sc0001207.1_g000032 Rmu_sc0001214.1_g000001 Rmu_sc0001323.1_g000017 Rmu_sc0001673.1_g000006 Rmu_sc0001785.1_g000018 Rmu_sc0001959.1_g000012 Rmu_sc0002073.1_g000006 Rmu_sc0002076.1_g000005 Rmu_sc0002094.1_g000004 Rmu_sc0002132.1_g000057 Rmu_sc0002170.1_g000036 Rmu_sc0002187.1_g000010 Rmu_sc0002316.1_g000037 Rmu_sc0002324.1_g000009 Rmu_sc0002367.1_g000001 Rmu_sc0002482.1_g000001 Rmu_sc0002547.1_g000017 Rmu_sc0002578.1_g000012 Rmu_sc0002693.1_g000016 Rmu_sc0002706.1_g000004 Rmu_sc0002759.1_g000032 Rmu_sc0002765.1_g000002 Rmu_sc0002845.1_g000026 Rmu_sc0002895.1_g000014 Rmu_sc0002983.1_g000013 Rmu_sc0003187.1_g000017 Rmu_sc0003249.1_g000029 Rmu_sc0003342.1_g000038 Rmu_sc0003374.1_g000002 Rmu_sc0003470.1_g000017 Rmu_sc0003669.1_g000013 Rmu_sc0003693.1_g000008 Rmu_sc0003749.1_g000015 Rmu_sc0003833.1_g000005 Rmu_sc0003936.1_g000036 Rmu_sc0004798.1_g000001 Rmu_sc0004932.1_g000024 Rmu_sc0005088.1_g000004 Rmu_sc0005439.1_g000003 Rmu_sc0005532.1_g000002 Rmu_sc0005728.1_g000009 Rmu_sc0005879.1_g000012 Rmu_sc0005914.1_g000010 Rmu_sc0006112.1_g000002 Rmu_sc0006196.1_g000003 Rmu_sc0006248.1_g000001 Rmu_sc0006366.1_g000015 Rmu_sc0006567.1_g000015 Rmu_sc0006695.1_g000070 Rmu_sc0006937.1_g000004 Rmu_sc0007647.1_g000009 Rmu_sc0008681.1_g000001 Rmu_sc0009833.1_g000019 Rmu_sc0010256.1_g000006 Rmu_sc0010366.1_g000001 Rmu_sc0010462.1_g000006 Rmu_sc0010817.1_g000017 Rmu_sc0014139.1_g000002 Rmu_sc0015191.1_g000001 Rmu_sc0015649.1_g000002 Rmu_sc0017650.1_g000005 Rmu_sc0028733.1_g000002 Rmu_ssc0000024.1_g000019 Rmu_ssc0000134.1_g000059 Rmu_ssc0000400.1_g000040 Rmu_ssc0000438.1_g000049
rosa_roxburghii Rroxscaffold_3G00231160 Rroxscaffold_5G00354700 Rroxscaffold_6G00395540
rosa_rugosa Rorug01G0149700.1 Rorug03G0299500 Rorug07G0249000
rosa_samantha Rh3BG164500 Rh3BG164800 Rh6BG099500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 242
AclWI GGATC 2 cut(s) 58, 71
AcoI YGGCCR 2 cut(s) 559, 880
AcsI RAATTY 1 cut(s) 438
AcuI CTGAAG 1 cut(s) 648
AfaI GTAC 1 cut(s) 554
AfiI CCNNNNNNNGG 1 cut(s) 549
AgsI TTSAA 7 cut(s) 58, 353, 434, 485, 521, 536, 782
AjnI CCWGG 1 cut(s) 132
AjuI GAANNNNNNNTTGG 2 cut(s) 311, 343
AluBI AGCT 3 cut(s) 308, 383, 398
AluI AGCT 3 cut(s) 308, 383, 398
Alw26I GTCTC 2 cut(s) 162, 295
AlwI GGATC 2 cut(s) 58, 71
AoxI GGCC 5 cut(s) 422, 559, 591, 849, 880
ApeKI GCWGC 3 cut(s) 200, 203, 653
ApoI RAATTY 1 cut(s) 438
Asp700I GAANNNNTTC 2 cut(s) 438, 489
AspS9I GGNCC 1 cut(s) 850
AsuHPI GGTGA 2 cut(s) 305, 488
BaeI ACNNNNGTAYC 4 cut(s) 267, 300, 804, 837
BalI TGGCCA 2 cut(s) 561, 882
BamHI GGATCC 1 cut(s) 63
BbvI GCAGC 3 cut(s) 187, 190, 665
BceAI ACGGC 2 cut(s) 180, 213
BcgI CGANNNNNNTGC 2 cut(s) 812, 846
BciT130I CCWGG 1 cut(s) 134
BclI TGATCA 1 cut(s) 124
BcoDI GTCTC 2 cut(s) 162, 295
BfaI CTAG 4 cut(s) 305, 395, 420, 602
BisI GCNGC 3 cut(s) 201, 204, 654
BlsI GCNGC 3 cut(s) 202, 205, 655
Bme1390I CCNGG 1 cut(s) 134
BmgT120I GGNCC 1 cut(s) 850
BmiI GGNNCC 1 cut(s) 65
BmrFI CCNGG 1 cut(s) 134
BmrI ACTGGG 1 cut(s) 47
BmsI GCATC 1 cut(s) 585
BmuI ACTGGG 1 cut(s) 47
BpmI CTGGAG 1 cut(s) 255
BpuEI CTTGAG 1 cut(s) 556
BsaJI CCNNGG 3 cut(s) 132, 133, 581
BsaXI ACNNNNNCTCC 2 cut(s) 851, 881
Bsc4I CCNNNNNNNGG 1 cut(s) 549
Bse1I ACTGG 5 cut(s) 42, 154, 238, 298, 756
BseBI CCWGG 1 cut(s) 134
BseDI CCNNGG 3 cut(s) 132, 133, 581
BseGI GGATG 1 cut(s) 576
BseLI CCNNNNNNNGG 1 cut(s) 549
BseNI ACTGG 5 cut(s) 42, 154, 238, 298, 756
BseXI GCAGC 3 cut(s) 187, 190, 665
BseYI CCCAGC 1 cut(s) 379
BshFI GGCC 5 cut(s) 424, 561, 593, 851, 882
BslI CCNNNNNNNGG 1 cut(s) 549
BsmAI GTCTC 2 cut(s) 162, 295
BsnI GGCC 5 cut(s) 424, 561, 593, 851, 882
Bsp143I GATC 4 cut(s) 63, 124, 618, 685
Bsp19I CCATGG 1 cut(s) 581
BspANI GGCC 5 cut(s) 424, 561, 593, 851, 882
BspLI GGNNCC 1 cut(s) 65
BspPI GGATC 2 cut(s) 58, 71
BsrI ACTGG 5 cut(s) 42, 154, 238, 298, 756
BssECI CCNNGG 3 cut(s) 132, 133, 581
BssMI GATC 4 cut(s) 63, 124, 618, 685
BssT1I CCWWGG 1 cut(s) 581
Bst2UI CCWGG 1 cut(s) 134
Bst4CI ACNGT 5 cut(s) 173, 192, 429, 476, 841
Bst6I CTCTTC 1 cut(s) 53
BstC8I GCNNGC 2 cut(s) 757, 761
BstDEI CTNAG 1 cut(s) 98
BstDSI CCRYGG 1 cut(s) 581
BstF5I GGATG 1 cut(s) 576
BstKTI GATC 4 cut(s) 66, 127, 621, 688
BstMAI GTCTC 2 cut(s) 162, 295
BstMBI GATC 4 cut(s) 63, 124, 618, 685
BstMWI GCNNNNNNNGC 1 cut(s) 389
BstNI CCWGG 1 cut(s) 134
BstNSI RCATGY 1 cut(s) 759
BstSCI CCNGG 1 cut(s) 132
BstV1I GCAGC 3 cut(s) 187, 190, 665
BstX2I RGATCY 1 cut(s) 63
BstYI RGATCY 1 cut(s) 63
BsuRI GGCC 5 cut(s) 424, 561, 593, 851, 882
BtgI CCRYGG 1 cut(s) 581
BtsCI GGATG 1 cut(s) 576
BtsI GCAGTG 3 cut(s) 675, 712, 770
BtsIMutI CAGTG 5 cut(s) 675, 712, 749, 770, 837
Cac8I GCNNGC 2 cut(s) 757, 761
Cfr13I GGNCC 1 cut(s) 850
Csp6I GTAC 1 cut(s) 553
CviAII CATG 4 cut(s) 49, 582, 595, 756
CviQI GTAC 1 cut(s) 553
DdeI CTNAG 1 cut(s) 98
DpnI GATC 4 cut(s) 65, 126, 620, 687
DpnII GATC 4 cut(s) 63, 124, 618, 685
EaeI YGGCCR 2 cut(s) 559, 880
Eam1104I CTCTTC 1 cut(s) 53
EarI CTCTTC 1 cut(s) 53
Eco130I CCWWGG 1 cut(s) 581
Eco32I GATATC 1 cut(s) 634
Eco57I CTGAAG 1 cut(s) 648
EcoRII CCWGG 1 cut(s) 132
EcoRV GATATC 1 cut(s) 634
EcoT14I CCWWGG 1 cut(s) 581
ErhI CCWWGG 1 cut(s) 581
FaeI CATG 4 cut(s) 52, 585, 598, 759
FatI CATG 4 cut(s) 48, 581, 594, 755
FauNDI CATATG 1 cut(s) 898
FbaI TGATCA 1 cut(s) 124
Fnu4HI GCNGC 3 cut(s) 201, 204, 654
FokI GGATG 1 cut(s) 563
Fsp4HI GCNGC 3 cut(s) 201, 204, 654
FspBI CTAG 4 cut(s) 305, 395, 420, 602
GluI GCNGC 3 cut(s) 201, 204, 654
GsaI CCCAGC 1 cut(s) 383
GsuI CTGGAG 1 cut(s) 255
HaeIII GGCC 5 cut(s) 424, 561, 593, 851, 882
Hin1II CATG 4 cut(s) 52, 585, 598, 759
HincII GTYRAC 1 cut(s) 195
HindII GTYRAC 1 cut(s) 195
HinfI GANTC 2 cut(s) 598, 743
HphI GGTGA 2 cut(s) 305, 488
Hpy166II GTNNAC 1 cut(s) 195
Hpy188I TCNGA 4 cut(s) 290, 372, 628, 892
Hpy188III TCNNGA 6 cut(s) 186, 353, 463, 602, 613, 692
Hpy8I GTNNAC 1 cut(s) 195
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 4 cut(s) 4, 313, 460, 655
HpyCH4III ACNGT 5 cut(s) 173, 192, 429, 476, 841
HpyCH4IV ACGT 1 cut(s) 696
HpyCH4V TGCA 2 cut(s) 392, 763
HpyF10VI GCNNNNNNNGC 1 cut(s) 389
HpyF3I CTNAG 1 cut(s) 98
HpySE526I ACGT 1 cut(s) 696
Hsp92II CATG 4 cut(s) 52, 585, 598, 759
Ksp22I TGATCA 1 cut(s) 124
Kzo9I GATC 4 cut(s) 63, 124, 618, 685
Lsp1109I GCAGC 3 cut(s) 187, 190, 665
LweI GCATC 1 cut(s) 585
MaeI CTAG 4 cut(s) 305, 395, 420, 602
MaeII ACGT 1 cut(s) 696
MaeIII GTNAC 1 cut(s) 707
MalI GATC 4 cut(s) 65, 126, 620, 687
MboI GATC 4 cut(s) 63, 124, 618, 685
MboII GAAGA 7 cut(s) 44, 70, 446, 524, 628, 641, 836
MflI RGATCY 1 cut(s) 63
MlsI TGGCCA 2 cut(s) 561, 882
MluCI AATT 6 cut(s) 253, 260, 324, 438, 513, 886
MluNI TGGCCA 2 cut(s) 561, 882
MlyI GAGTC 1 cut(s) 592
MmeI TCCRAC 1 cut(s) 254
MnlI CCTC 3 cut(s) 54, 92, 504
Mox20I TGGCCA 2 cut(s) 561, 882
MroXI GAANNNNTTC 2 cut(s) 438, 489
MscI TGGCCA 2 cut(s) 561, 882
MseI TTAA 2 cut(s) 252, 263
MslI CAYNNNNRTG 1 cut(s) 754
Msp20I TGGCCA 2 cut(s) 561, 882
MspR9I CCNGG 1 cut(s) 134
MvaI CCWGG 1 cut(s) 134
MwoI GCNNNNNNNGC 1 cut(s) 389
NcoI CCATGG 1 cut(s) 581
NdeI CATATG 1 cut(s) 898
NdeII GATC 4 cut(s) 63, 124, 618, 685
NlaIII CATG 4 cut(s) 52, 585, 598, 759
NlaIV GGNNCC 1 cut(s) 65
NmuCI GTSAC 1 cut(s) 707
NspI RCATGY 1 cut(s) 759
PaeI GCATGC 1 cut(s) 759
PasI CCCWGGG 1 cut(s) 133
PdmI GAANNNNTTC 2 cut(s) 438, 489
PfeI GAWTC 1 cut(s) 743
PkrI GCNGC 3 cut(s) 202, 205, 655
PleI GAGTC 1 cut(s) 592
PpsI GAGTC 1 cut(s) 592
PsiI TTATAA 1 cut(s) 242
Psp6I CCWGG 1 cut(s) 132
PspFI CCCAGC 1 cut(s) 379
PspGI CCWGG 1 cut(s) 132
PspN4I GGNNCC 1 cut(s) 65
PspPI GGNCC 1 cut(s) 850
PsrI GAACNNNNNNTAC 2 cut(s) 259, 291
PsuI RGATCY 1 cut(s) 63
RsaI GTAC 1 cut(s) 554
RsaNI GTAC 1 cut(s) 553
RseI CAYNNNNRTG 1 cut(s) 754
SaqAI TTAA 2 cut(s) 252, 263
SatI GCNGC 3 cut(s) 201, 204, 654
Sau3AI GATC 4 cut(s) 63, 124, 618, 685
Sau96I GGNCC 1 cut(s) 850
SchI GAGTC 1 cut(s) 592
ScrFI CCNGG 1 cut(s) 134
SetI ASST 8 cut(s) 15, 92, 310, 385, 400, 647, 699, 714
SfaNI GCATC 1 cut(s) 585
SmiMI CAYNNNNRTG 1 cut(s) 754
SmlI CTYRAG 1 cut(s) 571
SmoI CTYRAG 1 cut(s) 571
SphI GCATGC 1 cut(s) 759
Sse9I AATT 6 cut(s) 253, 260, 324, 438, 513, 886
SspMI CTAG 4 cut(s) 305, 395, 420, 602
StyD4I CCNGG 1 cut(s) 132
StyI CCWWGG 1 cut(s) 581
TaaI ACNGT 5 cut(s) 173, 192, 429, 476, 841
TaiI ACGT 1 cut(s) 699
TaqI TCGA 2 cut(s) 746, 822
TasI AATT 6 cut(s) 253, 260, 324, 438, 513, 886
TfiI GAWTC 1 cut(s) 743
Tru1I TTAA 2 cut(s) 252, 263
Tru9I TTAA 2 cut(s) 252, 263
TscAI CASTG 5 cut(s) 682, 712, 756, 770, 844
TseFI GTSAC 1 cut(s) 707
TseI GCWGC 3 cut(s) 200, 203, 653
Tsp45I GTSAC 1 cut(s) 707
TspDTI ATGAA 4 cut(s) 65, 273, 426, 431
TspRI CASTG 5 cut(s) 682, 712, 756, 770, 844
XapI RAATTY 1 cut(s) 438
XbaI TCTAGA 1 cut(s) 601
XceI RCATGY 1 cut(s) 759
XcmI CCANNNNNNNNNTGG 1 cut(s) 844
XmnI GAANNNNTTC 2 cut(s) 438, 489
XspI CTAG 4 cut(s) 305, 395, 420, 602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.