FvH4_1g00344

2-alkenal reductase (NADP( )-dependent)-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000196
Physical Location & Seq
Forward (+)
184980 .. 187276
2297 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g00344.t1

Sequence Viewer

Length: 1035 bp
ATGGCGAGCGAGATGAGCAACAAGCAGGTGATATTCAAAGACTATGTGACCGGGTTCCCCAAAGAATCCGACATGGAATTGAGGACGTCCACAGCCAAACTCAAGCTTCCACAAGGGTCGACTGGCCTTGTCGTCAAGAACCTATACTTGTCCTGCGATCCTTACTTACGCGGCCATATGACCAAGCACGACAGGCCCTCTTATGTCCAATCCTTCAACCCCGGCCAGACTGTAACTGGTTATGGAGTGGCTAAAGTCCTGGAATCTGGGGATCCAAAGTTTAAGCCAGGCGACTTGGTTTGGGGTCATACTGGCTGGGAAGAATATAGTGTCATCACTGCCACAGAGTCTCTGTTTAAAATTCACCACACTGATGTGCCTCTCTCTTACTATACTGGACTTCTCGGTATGCCTGGAATGACTGCTTATGCTGGGTTTTTTGAGGTTTGCTCTCCTAAGAAAGGAGAGACTGTCTACATTTCAGCCGCATCTGGAGCAGTAGGTCAGCTTGTTGGCCAATTTGCAAAGTTGACAGGTTGCTATGTTGTTGGGAGTGCTGGAAGCAAGGAAAAGGTTGATCTGCTGAAGAACAAATTCGGATTTGATGAGGCTTTCAACTATAAAGACGAACCTGACCTGGATGCAGCTTTAAGAAGGTATTTTCCTGATGGTATTGACATTTACTTTGAGAATGTGGGGGGAAAGATGCTTGATGCAGTGCTACCAAACATGAGGCTGAAAGGGCGAATAGCAGTGTGTGGGATGATATCTCAGTACAACTTGGAGAAGCCTGAAGGCATACATAATTTGATGTCTCTGTTTGTTAAGCAGGTCCGCATGGAAGGTTTCATTGTGACCCTTTACTATCATCTGTATGCAAAGTTTCTTGAAACAGTGTTGCCTGCCTTAAAAGAAGGGAAGATTACATATGTGGAAGATGTAGTTGAAGGCCTTGAGAACGCTCCTGCGGCTCTAATTGGGCTCTTTGCTGGCCGCAACGTGGGAAAGCAGGTTGTTGTAGTTTCCAGGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006950 GO:0006979 GO:0008150 GO:0016020 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N_2 ADH_zinc_N

Protein Analysis

345

Amino Acids

37.96

Weight (kDa)

6.67

Isoelectric Point (pI)

29.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N_2 PF16884 8 - 116 2.8e-26 N-terminal domain of oxidoreductase
ADH_zinc_N PF00107 166 - 296 4.1e-23 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 200 - 339 2.7e-09 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000196)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65560
fragaria_vesca FvH4_1g00330 FvH4_1g00342 FvH4_1g00343 FvH4_1g00344 FvH4_1g00345 FvH4_1g00345 FvH4_1g00346 FvH4_1g26520
malus_domestica MD02G1001800.v1.1 MD02G1001900.v1.1 MD02G1002100.v1.1 MD02G1002300.v1.1 MD02G1002400.v1.1 MD02G1002500.v1.1 MD02G1002600.v1.1 MD15G1145700.v1.1
prunus_persica Prupe.7G268600_v2.0.a1 Prupe.7G268700_v2.0.a1 Prupe.7G268800_v2.0.a1 Prupe.7G268800_v2.0.a1 Prupe.7G268900_v2.0.a1
pyrus_communis pycom02g00050 pycom02g00060 pycom02g00100 pycom02g00120 pycom02g00130 pycom15g13090 pycom15g13100
rosa_chinensis RchiOBHm_Chr1g0325321 RchiOBHm_Chr1g0325341 RchiOBHm_Chr1g0325351 RchiOBHm_Chr1g0325361 RchiOBHm_Chr1g0364881 RchiOBHm_Chr1g0364891 RchiOBHm_Chr2g0084701 RchiOBHm_Chr2g0084721 RchiOBHm_Chr2g0084731 RchiOBHm_Chr2g0084741 RchiOBHm_Chr2g0084751 RchiOBHm_Chr2g0084761 RchiOBHm_Chr2g0084771 RchiOBHm_Chr2g0085311 RchiOBHm_Chr2g0085321 RchiOBHm_Chr2g0085341 RchiOBHm_Chr2g0085351 RchiOBHm_Chr2g0117721 RchiOBHm_Chr2g0159631 RchiOBHm_Chr3g0484011 RchiOBHm_Chr5g0021141 RchiOBHm_Chr5g0061081 RchiOBHm_Chr6g0275731 RchiOBHm_Chr7g0238011
rosa_laevigata RLG00000013438 RLG00000015632 RLG00000015634 RLG00000015635 RLG00000015636 RLG00000015637 RLG00000015677 RLG00000015679 RLG00000030145 RLG00000030146 RLG00000035427
rosa_multiflora Rmu_co8334849.1_g000001 Rmu_sc0001366.1_g000035 Rmu_sc0002352.1_g000003 Rmu_sc0002352.1_g000004 Rmu_sc0002352.1_g000006 Rmu_sc0002352.1_g000007 Rmu_sc0002352.1_g000009 Rmu_sc0002586.1_g000008 Rmu_sc0004966.1_g000016 Rmu_sc0007122.1_g000001 Rmu_sc0008804.1_g000002 Rmu_sc0008883.1_g000001 Rmu_sc0010860.1_g000002 Rmu_sc0012119.1_g000001 Rmu_sc0012119.1_g000003 Rmu_sc0012119.1_g000006 Rmu_sc0012119.1_g000007 Rmu_sc0012119.1_g000009 Rmu_sc0020031.1_g000004 Rmu_sc0020800.1_g000005 Rmu_sc0020800.1_g000007 Rmu_sc0020800.1_g000008 Rmu_sc0020800.1_g000009 Rmu_sc0025597.1_g000001 Rmu_sc0029132.1_g000002
rosa_roxburghii Rroxscaffold_2G00155450 Rroxscaffold_2G00155460 Rroxscaffold_2G00155470 Rroxscaffold_2G00155830 Rroxscaffold_2G00155840 Rroxscaffold_2G00155850 Rroxscaffold_2G00155860 Rroxscaffold_2G00155870 Rroxscaffold_2G00155910 Rroxscaffold_3G00273850 Rroxscaffold_4G00324820 Rroxscaffold_7G00192930 Rroxscaffold_7G00192960
rosa_rugosa Rorug01G0051300 Rorug01G0051400 Rorug01G0455700 Rorug01G0455800 Rorug01G0455900 Rorug01G0459500 Rorug01G0459500 Rorug01G0459600 Rorug01G0459700 Rorug01G0459700 Rorug02G0335000
rosa_samantha Rh1AG066500 Rh1BG054700 Rh1BG054800 Rh1BG176600 Rh2BG004400 Rh2BG004500 Rh2BG004600 Rh2BG004700 Rh2BG008600 Rh2BG008700 Rh2CG004500 Rh2CG004700 Rh2CG004800 Rh2CG004900 Rh2CG005000 Rh2CG005100 Rh2CG005200 Rh2CG009300 Rh2CG009400 Rh2DG004200 Rh2DG004300 Rh2DG004400 Rh2DG004500 Rh2DG004600 Rh2DG009800 Rh5BG411800 Rh5CG167200 Rh5CG436800 Rh5CG578800 Rh6DG203800
rosa_wichuraiana Rw1G005560 Rw2G000320 Rw2G000350 Rw2G000360 Rw2G000370 Rw2G000750 Rw2G000760 Rw4G022130 Rw5G015810 Rw5G037620 Rw6G018120 Rw7G038530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 16
AatII GACGTC 1 cut(s) 89
Acc36I ACCTGC 3 cut(s) 16, 820, 1000
AccI GTMKAC 2 cut(s) 119, 474
AccII CGCG 1 cut(s) 171
AciI CCGC 5 cut(s) 171, 486, 835, 968, 994
AclWI GGATC 3 cut(s) 152, 266, 279
AcoI YGGCCR 4 cut(s) 172, 223, 514, 991
AcsI RAATTY 2 cut(s) 360, 593
AcuI CTGAAG 2 cut(s) 605, 813
AcyI GRCGYC 1 cut(s) 86
AfaI GTAC 1 cut(s) 776
AfiI CCNNNNNNNGG 2 cut(s) 461, 1000
AgsI TTSAA 5 cut(s) 37, 217, 616, 890, 947
AjnI CCWGG 5 cut(s) 258, 286, 412, 636, 1025
AleI CACNNNNGTG 1 cut(s) 374
AluBI AGCT 3 cut(s) 106, 508, 647
AluI AGCT 3 cut(s) 106, 508, 647
Alw26I GTCTC 3 cut(s) 354, 461, 819
AlwI GGATC 3 cut(s) 152, 266, 279
AoxI GGCC 7 cut(s) 124, 172, 194, 223, 514, 949, 991
ApeKI GCWGC 1 cut(s) 644
ApoI RAATTY 2 cut(s) 360, 593
ArsI GACNNNNNNTTYG 2 cut(s) 668, 700
Asp700I GAANNNNTTC 1 cut(s) 593
AspS9I GGNCC 2 cut(s) 195, 832
AsuC2I CCSGG 2 cut(s) 52, 222
AsuHPI GGTGA 2 cut(s) 40, 356
AvaII GGWCC 1 cut(s) 832
BalI TGGCCA 1 cut(s) 516
BamHI GGATCC 1 cut(s) 271
BanII GRGCYC 1 cut(s) 984
BbvI GCAGC 1 cut(s) 656
BccI CCATC 1 cut(s) 662
BciT130I CCWGG 5 cut(s) 260, 288, 414, 638, 1027
BcnI CCSGG 2 cut(s) 52, 222
BcoDI GTCTC 3 cut(s) 354, 461, 819
BfuAI ACCTGC 3 cut(s) 16, 820, 1000
BisI GCNGC 5 cut(s) 172, 486, 645, 969, 994
BlsI GCNGC 5 cut(s) 173, 487, 646, 970, 995
Bme1390I CCNGG 7 cut(s) 52, 222, 260, 288, 414, 638, 1027
Bme18I GGWCC 1 cut(s) 832
BmgT120I GGNCC 2 cut(s) 195, 832
BmiI GGNNCC 2 cut(s) 56, 273
BmrFI CCNGG 7 cut(s) 52, 222, 260, 288, 414, 638, 1027
BmsI GCATC 4 cut(s) 497, 631, 696, 703
BplI GAGNNNNNCTC 2 cut(s) 434, 466
BpmI CTGGAG 1 cut(s) 513
BpuEI CTTGAG 2 cut(s) 86, 974
BpuMI CCSGG 2 cut(s) 52, 222
BsaHI GRCGYC 1 cut(s) 86
BsaJI CCNNGG 2 cut(s) 220, 1026
BsaXI ACNNNNNCTCC 2 cut(s) 456, 486
Bsc4I CCNNNNNNNGG 2 cut(s) 461, 1000
Bse1I ACTGG 4 cut(s) 127, 241, 316, 400
BseBI CCWGG 5 cut(s) 260, 288, 414, 638, 1027
BseDI CCNNGG 2 cut(s) 220, 1026
BseGI GGATG 2 cut(s) 646, 768
BseLI CCNNNNNNNGG 2 cut(s) 461, 1000
BseMII CTCAG 1 cut(s) 785
BseNI ACTGG 4 cut(s) 127, 241, 316, 400
BseXI GCAGC 1 cut(s) 656
BseYI CCCAGC 2 cut(s) 315, 431
Bsh1236I CGCG 1 cut(s) 171
BshFI GGCC 7 cut(s) 126, 174, 196, 225, 516, 951, 993
BsiSI CCGG 2 cut(s) 51, 222
BslI CCNNNNNNNGG 2 cut(s) 461, 1000
BsmAI GTCTC 3 cut(s) 354, 461, 819
BsnI GGCC 7 cut(s) 126, 174, 196, 225, 516, 951, 993
Bsp1286I GDGCHC 1 cut(s) 984
Bsp143I GATC 3 cut(s) 157, 271, 577
BspACI CCGC 5 cut(s) 171, 486, 835, 968, 994
BspANI GGCC 7 cut(s) 126, 174, 196, 225, 516, 951, 993
BspCNI CTCAG 1 cut(s) 784
BspFNI CGCG 1 cut(s) 171
BspLI GGNNCC 2 cut(s) 56, 273
BspMI ACCTGC 3 cut(s) 16, 820, 1000
BspPI GGATC 3 cut(s) 152, 266, 279
BsrI ACTGG 4 cut(s) 127, 241, 316, 400
BssECI CCNNGG 2 cut(s) 220, 1026
BssMI GATC 3 cut(s) 157, 271, 577
BssNI GRCGYC 1 cut(s) 86
Bst2UI CCWGG 5 cut(s) 260, 288, 414, 638, 1027
Bst4CI ACNGT 3 cut(s) 232, 472, 895
BstACI GRCGYC 1 cut(s) 86
BstC8I GCNNGC 3 cut(s) 7, 903, 991
BstDEI CTNAG 2 cut(s) 456, 771
BstENI CCTNNNNNAGG 1 cut(s) 459
BstF5I GGATG 2 cut(s) 646, 768
BstFNI CGCG 1 cut(s) 171
BstKTI GATC 3 cut(s) 160, 274, 580
BstMAI GTCTC 3 cut(s) 354, 461, 819
BstMBI GATC 3 cut(s) 157, 271, 577
BstMWI GCNNNNNNNGC 5 cut(s) 15, 193, 494, 742, 968
BstNI CCWGG 5 cut(s) 260, 288, 414, 638, 1027
BstSCI CCNGG 7 cut(s) 50, 220, 258, 286, 412, 636, 1025
BstUI CGCG 1 cut(s) 171
BstV1I GCAGC 1 cut(s) 656
BstX2I RGATCY 1 cut(s) 271
BstYI RGATCY 1 cut(s) 271
BsuRI GGCC 7 cut(s) 126, 174, 196, 225, 516, 951, 993
BtsCI GGATG 2 cut(s) 646, 768
BtsI GCAGTG 3 cut(s) 336, 723, 759
BtsIMutI CAGTG 5 cut(s) 336, 369, 723, 759, 900
BveI ACCTGC 3 cut(s) 16, 820, 1000
Cac8I GCNNGC 3 cut(s) 7, 903, 991
Cfr13I GGNCC 2 cut(s) 195, 832
Csp6I GTAC 1 cut(s) 775
CviAII CATG 3 cut(s) 73, 730, 838
CviQI GTAC 1 cut(s) 775
DdeI CTNAG 2 cut(s) 456, 771
DpnI GATC 3 cut(s) 159, 273, 579
DpnII GATC 3 cut(s) 157, 271, 577
DraI TTTAAA 1 cut(s) 358
EaeI YGGCCR 4 cut(s) 172, 223, 514, 991
Eco147I AGGCCT 1 cut(s) 951
Eco24I GRGCYC 1 cut(s) 984
Eco32I GATATC 1 cut(s) 768
Eco47I GGWCC 1 cut(s) 832
Eco57I CTGAAG 2 cut(s) 605, 813
EcoNI CCTNNNNNAGG 1 cut(s) 459
EcoO109I RGGNCCY 1 cut(s) 195
EcoRII CCWGG 5 cut(s) 258, 286, 412, 636, 1025
EcoRV GATATC 1 cut(s) 768
EcoT38I GRGCYC 1 cut(s) 984
FaeI CATG 3 cut(s) 76, 733, 841
FatI CATG 3 cut(s) 72, 729, 837
FauNDI CATATG 2 cut(s) 177, 928
FblI GTMKAC 2 cut(s) 119, 474
Fnu4HI GCNGC 5 cut(s) 172, 486, 645, 969, 994
FokI GGATG 2 cut(s) 653, 775
FriOI GRGCYC 1 cut(s) 984
Fsp4HI GCNGC 5 cut(s) 172, 486, 645, 969, 994
GluI GCNGC 5 cut(s) 172, 486, 645, 969, 994
GsaI CCCAGC 2 cut(s) 319, 435
GsuI CTGGAG 1 cut(s) 513
HaeIII GGCC 7 cut(s) 126, 174, 196, 225, 516, 951, 993
HapII CCGG 2 cut(s) 51, 222
Hin1I GRCGYC 1 cut(s) 86
Hin1II CATG 3 cut(s) 76, 733, 841
HincII GTYRAC 2 cut(s) 120, 531
HindII GTYRAC 2 cut(s) 120, 531
HindIII AAGCTT 1 cut(s) 104
HinfI GANTC 3 cut(s) 65, 263, 347
HpaII CCGG 2 cut(s) 51, 222
HphI GGTGA 2 cut(s) 40, 356
Hpy166II GTNNAC 4 cut(s) 90, 120, 475, 531
Hpy188I TCNGA 2 cut(s) 70, 599
Hpy188III TCNNGA 4 cut(s) 136, 492, 665, 887
Hpy8I GTNNAC 4 cut(s) 90, 120, 475, 531
HpyAV CCTTC 6 cut(s) 223, 648, 788, 836, 908, 941
HpyCH4III ACNGT 3 cut(s) 232, 472, 895
HpyCH4IV ACGT 2 cut(s) 86, 999
HpyCH4V TGCA 4 cut(s) 524, 644, 716, 878
HpyF10VI GCNNNNNNNGC 5 cut(s) 15, 193, 494, 742, 968
HpyF3I CTNAG 2 cut(s) 456, 771
HpySE526I ACGT 2 cut(s) 86, 999
Hsp92I GRCGYC 1 cut(s) 86
Hsp92II CATG 3 cut(s) 76, 733, 841
Kzo9I GATC 3 cut(s) 157, 271, 577
LmnI GCTCC 2 cut(s) 494, 967
Lsp1109I GCAGC 1 cut(s) 656
LweI GCATC 4 cut(s) 497, 631, 696, 703
MaeII ACGT 2 cut(s) 86, 999
MaeIII GTNAC 3 cut(s) 46, 232, 853
MalI GATC 3 cut(s) 159, 273, 579
MboI GATC 3 cut(s) 157, 271, 577
MboII GAAGA 4 cut(s) 332, 598, 931, 947
MflI RGATCY 1 cut(s) 271
MhlI GDGCHC 1 cut(s) 984
MlsI TGGCCA 1 cut(s) 516
MluCI AATT 6 cut(s) 77, 360, 518, 593, 805, 975
MluNI TGGCCA 1 cut(s) 516
MlyI GAGTC 1 cut(s) 356
MmeI TCCRAC 1 cut(s) 93
MnlI CCTC 6 cut(s) 75, 208, 390, 436, 601, 726
Mox20I TGGCCA 1 cut(s) 516
MroXI GAANNNNTTC 1 cut(s) 593
MscI TGGCCA 1 cut(s) 516
MseI TTAA 5 cut(s) 282, 357, 650, 825, 908
MslI CAYNNNNRTG 3 cut(s) 372, 374, 873
Msp20I TGGCCA 1 cut(s) 516
MspI CCGG 2 cut(s) 51, 222
MspR9I CCNGG 7 cut(s) 52, 222, 260, 288, 414, 638, 1027
MvaI CCWGG 5 cut(s) 260, 288, 414, 638, 1027
MvnI CGCG 1 cut(s) 171
MwoI GCNNNNNNNGC 5 cut(s) 15, 193, 494, 742, 968
NciI CCSGG 2 cut(s) 52, 222
NdeI CATATG 2 cut(s) 177, 928
NdeII GATC 3 cut(s) 157, 271, 577
NlaIII CATG 3 cut(s) 76, 733, 841
NlaIV GGNNCC 2 cut(s) 56, 273
NmuCI GTSAC 2 cut(s) 46, 853
OliI CACNNNNGTG 1 cut(s) 374
PaqCI CACCTGC 1 cut(s) 16
PceI AGGCCT 1 cut(s) 951
PdmI GAANNNNTTC 1 cut(s) 593
PfeI GAWTC 2 cut(s) 65, 263
PfoI TCCNGGA 1 cut(s) 258
PkrI GCNGC 5 cut(s) 173, 487, 646, 970, 995
PleI GAGTC 1 cut(s) 355
PpsI GAGTC 1 cut(s) 355
Psp6I CCWGG 5 cut(s) 258, 286, 412, 636, 1025
PspFI CCCAGC 2 cut(s) 315, 431
PspGI CCWGG 5 cut(s) 258, 286, 412, 636, 1025
PspN4I GGNNCC 2 cut(s) 56, 273
PspPI GGNCC 2 cut(s) 195, 832
PsuI RGATCY 1 cut(s) 271
RsaI GTAC 1 cut(s) 776
RsaNI GTAC 1 cut(s) 775
RseI CAYNNNNRTG 3 cut(s) 372, 374, 873
SalI GTCGAC 1 cut(s) 118
SaqAI TTAA 5 cut(s) 282, 357, 650, 825, 908
SatI GCNGC 5 cut(s) 172, 486, 645, 969, 994
Sau3AI GATC 3 cut(s) 157, 271, 577
Sau96I GGNCC 2 cut(s) 195, 832
SchI GAGTC 1 cut(s) 356
ScrFI CCNGG 7 cut(s) 52, 222, 260, 288, 414, 638, 1027
SduI GDGCHC 1 cut(s) 984
SfaNI GCATC 4 cut(s) 497, 631, 696, 703
SinI GGWCC 1 cut(s) 832
SmiMI CAYNNNNRTG 3 cut(s) 372, 374, 873
SmlI CTYRAG 2 cut(s) 101, 953
SmoI CTYRAG 2 cut(s) 101, 953
Sse9I AATT 6 cut(s) 77, 360, 518, 593, 805, 975
SseBI AGGCCT 1 cut(s) 951
SsiI CCGC 5 cut(s) 171, 486, 835, 968, 994
StuI AGGCCT 1 cut(s) 951
StyD4I CCNGG 7 cut(s) 50, 220, 258, 286, 412, 636, 1025
TaaI ACNGT 3 cut(s) 232, 472, 895
TaiI ACGT 2 cut(s) 89, 1002
TaqI TCGA 1 cut(s) 119
TasI AATT 6 cut(s) 77, 360, 518, 593, 805, 975
TatI WGTACW 1 cut(s) 774
TauI GCSGC 4 cut(s) 174, 488, 971, 996
TfiI GAWTC 2 cut(s) 65, 263
Tru1I TTAA 5 cut(s) 282, 357, 650, 825, 908
Tru9I TTAA 5 cut(s) 282, 357, 650, 825, 908
TscAI CASTG 5 cut(s) 343, 376, 723, 759, 900
TseFI GTSAC 2 cut(s) 46, 853
TseI GCWGC 1 cut(s) 644
Tsp45I GTSAC 2 cut(s) 46, 853
TspDTI ATGAA 1 cut(s) 838
TspRI CASTG 5 cut(s) 343, 376, 723, 759, 900
VpaK11BI GGWCC 1 cut(s) 832
XagI CCTNNNNNAGG 1 cut(s) 459
XapI RAATTY 2 cut(s) 360, 593
XcmI CCANNNNNNNNNTGG 1 cut(s) 233
XmiI GTMKAC 2 cut(s) 119, 474
XmnI GAANNNNTTC 1 cut(s) 593
ZraI GACGTC 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.