MD02G1002300.v1.1

2-alkenal reductase (NADP( )-dependent)-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000196
Physical Location & Seq
Forward (+)
195505 .. 198092
2588 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1002300.v1.1.491

Sequence Viewer

Length: 1038 bp
ATGGCGGCGAGTACAGAGGAAGTGATCAGCAACAAGCAGGTGCTACTGAAAGACTATGTGACCGGCTTCCCCAAAGAATCTGACATGCAACTCACCGCCGCCACCACCAAGCTCAAGCTTCCAGAAGGTTCAAAAGGGGTTCTGGTGAAGAACCTCTACTTGTCCTGCGACCCTTATATTAGAAACCGCATGACCAAGCGTGAACCTGGCGCCAGTTTTATGGATTCCTTCAAACCCGGTTCGCCTATAGTCGGATATGGAGTTGCCAAAGTTTTGGAATCCGGAGATCCCAAGTTTAAGCAAGGCGACTTCATTTGGGGAATAACCGGGTGGGAAGAGTACAGTGTTATCACTGCAACGGAGTCTTTGTTTAAGATTCAGCACACTGATGTGCCTCTCTCTTACTATACTGGTATTCTTGGTATGCCTGGAATGACTGCTTATGCTGGTTTTTATGAGGTCTGCAATCCTAAGAAGGGAGAGACGGTCTTCGTTTCAGCAGCATCTGGGGCGGTAGGTCAGCTTGTTGGCCAATTTGCAAAGTCGTTGGGTTGCTATGTTGTTGGAAGTGCTGGAAGCAAAGAAAAGGTTGATCTGCTGAAGAACAAGTTTGGGTTTGACAAAGCTTTCAATTATAAAGAAGAGCCTGATTTGGATGCAGCTTTAAAAAGGCATCGACATATACTTTTGAAAACGTTGGGGGAAAAAATGCTTGATGCAGTGCTACAAAACATGAGGCTCCATGGCCGGATTGCAGTTTGTGGGATGGTCTCGCAGTATAACCTTGACCAGCCTGAAGGTTGTCACAATTTAATGTATCTGATCATTAAACAGATTCGCATGCAAGGTTTCGGGGTTTCTAGTTACTACCACCTTTACGGAAAGTTTCTTGAAATGGTTCTGCCGGCCATAAAAGAAGGGAAGATAACTTATGTGGAAGATGTAGTGGAAGGCCTTGAGAGCGCTCCAGCTGCTCTGATAGGGCTCTTTGCCGGCCGCAATGTGGGAAAGCAGGTGGTGGTAGTTTCCCGAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006950 GO:0006979 GO:0008150 GO:0016020 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N_2 ADH_zinc_N

Protein Analysis

346

Amino Acids

37.93

Weight (kDa)

8.57

Isoelectric Point (pI)

32.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N_2 PF16884 12 - 121 5.6e-25 N-terminal domain of oxidoreductase
ADH_zinc_N PF00107 171 - 298 3.7e-19 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000196)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65560
fragaria_vesca FvH4_1g00330 FvH4_1g00342 FvH4_1g00343 FvH4_1g00344 FvH4_1g00345 FvH4_1g00345 FvH4_1g00346 FvH4_1g26520
malus_domestica MD02G1001800.v1.1 MD02G1001900.v1.1 MD02G1002100.v1.1 MD02G1002300.v1.1 MD02G1002400.v1.1 MD02G1002500.v1.1 MD02G1002600.v1.1 MD15G1145700.v1.1
prunus_persica Prupe.7G268600_v2.0.a1 Prupe.7G268700_v2.0.a1 Prupe.7G268800_v2.0.a1 Prupe.7G268800_v2.0.a1 Prupe.7G268900_v2.0.a1
pyrus_communis pycom02g00050 pycom02g00060 pycom02g00100 pycom02g00120 pycom02g00130 pycom15g13090 pycom15g13100
rosa_chinensis RchiOBHm_Chr1g0325321 RchiOBHm_Chr1g0325341 RchiOBHm_Chr1g0325351 RchiOBHm_Chr1g0325361 RchiOBHm_Chr1g0364881 RchiOBHm_Chr1g0364891 RchiOBHm_Chr2g0084701 RchiOBHm_Chr2g0084721 RchiOBHm_Chr2g0084731 RchiOBHm_Chr2g0084741 RchiOBHm_Chr2g0084751 RchiOBHm_Chr2g0084761 RchiOBHm_Chr2g0084771 RchiOBHm_Chr2g0085311 RchiOBHm_Chr2g0085321 RchiOBHm_Chr2g0085341 RchiOBHm_Chr2g0085351 RchiOBHm_Chr2g0117721 RchiOBHm_Chr2g0159631 RchiOBHm_Chr3g0484011 RchiOBHm_Chr5g0021141 RchiOBHm_Chr5g0061081 RchiOBHm_Chr6g0275731 RchiOBHm_Chr7g0238011
rosa_laevigata RLG00000013438 RLG00000015632 RLG00000015634 RLG00000015635 RLG00000015636 RLG00000015637 RLG00000015677 RLG00000015679 RLG00000030145 RLG00000030146 RLG00000035427
rosa_multiflora Rmu_co8334849.1_g000001 Rmu_sc0001366.1_g000035 Rmu_sc0002352.1_g000003 Rmu_sc0002352.1_g000004 Rmu_sc0002352.1_g000006 Rmu_sc0002352.1_g000007 Rmu_sc0002352.1_g000009 Rmu_sc0002586.1_g000008 Rmu_sc0004966.1_g000016 Rmu_sc0007122.1_g000001 Rmu_sc0008804.1_g000002 Rmu_sc0008883.1_g000001 Rmu_sc0010860.1_g000002 Rmu_sc0012119.1_g000001 Rmu_sc0012119.1_g000003 Rmu_sc0012119.1_g000006 Rmu_sc0012119.1_g000007 Rmu_sc0012119.1_g000009 Rmu_sc0020031.1_g000004 Rmu_sc0020800.1_g000005 Rmu_sc0020800.1_g000007 Rmu_sc0020800.1_g000008 Rmu_sc0020800.1_g000009 Rmu_sc0025597.1_g000001 Rmu_sc0029132.1_g000002
rosa_roxburghii Rroxscaffold_2G00155450 Rroxscaffold_2G00155460 Rroxscaffold_2G00155470 Rroxscaffold_2G00155830 Rroxscaffold_2G00155840 Rroxscaffold_2G00155850 Rroxscaffold_2G00155860 Rroxscaffold_2G00155870 Rroxscaffold_2G00155910 Rroxscaffold_3G00273850 Rroxscaffold_4G00324820 Rroxscaffold_7G00192930 Rroxscaffold_7G00192960
rosa_rugosa Rorug01G0051300 Rorug01G0051400 Rorug01G0455700 Rorug01G0455800 Rorug01G0455900 Rorug01G0459500 Rorug01G0459500 Rorug01G0459600 Rorug01G0459700 Rorug01G0459700 Rorug02G0335000
rosa_samantha Rh1AG066500 Rh1BG054700 Rh1BG054800 Rh1BG176600 Rh2BG004400 Rh2BG004500 Rh2BG004600 Rh2BG004700 Rh2BG008600 Rh2BG008700 Rh2CG004500 Rh2CG004700 Rh2CG004800 Rh2CG004900 Rh2CG005000 Rh2CG005100 Rh2CG005200 Rh2CG009300 Rh2CG009400 Rh2DG004200 Rh2DG004300 Rh2DG004400 Rh2DG004500 Rh2DG004600 Rh2DG009800 Rh5BG411800 Rh5CG167200 Rh5CG436800 Rh5CG578800 Rh6DG203800
rosa_wichuraiana Rw1G005560 Rw2G000320 Rw2G000350 Rw2G000360 Rw2G000370 Rw2G000750 Rw2G000760 Rw4G022130 Rw5G015810 Rw5G037620 Rw6G018120 Rw7G038530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 636
AarI CACCTGC 2 cut(s) 28, 1003
Acc36I ACCTGC 2 cut(s) 28, 1003
AccB1I GGYRCC 1 cut(s) 209
AccIII TCCGGA 1 cut(s) 281
AciI CCGC 6 cut(s) 5, 96, 99, 187, 512, 997
AclI AACGTT 1 cut(s) 695
AclWI GGATC 1 cut(s) 281
AcoI YGGCCR 4 cut(s) 529, 745, 906, 994
AcuI CTGAAG 2 cut(s) 620, 816
AcyI GRCGYC 1 cut(s) 210
AfaI GTAC 2 cut(s) 13, 341
AfeI AGCGCT 1 cut(s) 964
AfiI CCNNNNNNNGG 3 cut(s) 251, 476, 1003
AgsI TTSAA 5 cut(s) 132, 232, 631, 691, 893
AjnI CCWGG 2 cut(s) 205, 427
AleI CACNNNNGTG 1 cut(s) 389
AluBI AGCT 6 cut(s) 112, 118, 523, 626, 662, 971
AluI AGCT 6 cut(s) 112, 118, 523, 626, 662, 971
Alw26I GTCTC 2 cut(s) 476, 775
AlwI GGATC 1 cut(s) 281
AlwNI CAGNNNCTG 1 cut(s) 506
Ama87I CYCGRG 1 cut(s) 1029
Aor13HI TCCGGA 1 cut(s) 281
Aor51HI AGCGCT 1 cut(s) 964
AoxI GGCC 5 cut(s) 529, 745, 906, 952, 994
ApeKI GCWGC 3 cut(s) 500, 659, 971
Asp700I GAANNNNTTC 1 cut(s) 897
AspLEI GCGC 2 cut(s) 212, 965
AsuC2I CCSGG 2 cut(s) 237, 328
AsuHPI GGTGA 2 cut(s) 85, 157
AvaI CYCGRG 1 cut(s) 1029
BalI TGGCCA 1 cut(s) 531
BanI GGYRCC 1 cut(s) 209
BanII GRGCYC 1 cut(s) 987
BarI GAAGNNNNNNTAC 2 cut(s) 140, 172
BbsI GAAGAC 1 cut(s) 481
BbvI GCAGC 3 cut(s) 512, 671, 958
BccI CCATC 1 cut(s) 760
BciT130I CCWGG 2 cut(s) 207, 429
BclI TGATCA 2 cut(s) 24, 822
BcnI CCSGG 2 cut(s) 237, 328
BcoDI GTCTC 2 cut(s) 476, 775
BfaI CTAG 1 cut(s) 861
BfmI CTRYAG 1 cut(s) 246
BfoI RGCGCY 2 cut(s) 213, 966
BfuAI ACCTGC 2 cut(s) 28, 1003
BisI GCNGC 6 cut(s) 6, 99, 501, 660, 972, 997
BlsI GCNGC 6 cut(s) 7, 100, 502, 661, 973, 998
Bme1390I CCNGG 4 cut(s) 207, 237, 328, 429
BmeT110I CYCGRG 1 cut(s) 1029
BmiI GGNNCC 2 cut(s) 211, 740
BmrFI CCNGG 4 cut(s) 207, 237, 328, 429
BmsI GCATC 4 cut(s) 512, 646, 682, 706
BpiI GAAGAC 1 cut(s) 481
BpmI CTGGAG 1 cut(s) 951
BpuEI CTTGAG 2 cut(s) 98, 977
BpuMI CCSGG 2 cut(s) 237, 328
BsaHI GRCGYC 1 cut(s) 210
BsaI GGTCTC 1 cut(s) 775
BsaJI CCNNGG 1 cut(s) 742
BsaWI WCCGGW 1 cut(s) 281
BsaXI ACNNNNNCTCC 4 cut(s) 353, 383, 471, 501
Bsc4I CCNNNNNNNGG 3 cut(s) 251, 476, 1003
Bse118I RCCGGY 3 cut(s) 62, 904, 992
Bse1I ACTGG 2 cut(s) 213, 415
Bse3DI GCAATG 1 cut(s) 1006
BseAI TCCGGA 1 cut(s) 281
BseBI CCWGG 2 cut(s) 207, 429
BseDI CCNNGG 1 cut(s) 742
BseGI GGATG 2 cut(s) 661, 771
BseLI CCNNNNNNNGG 3 cut(s) 251, 476, 1003
BseMI GCAATG 1 cut(s) 1006
BseNI ACTGG 2 cut(s) 213, 415
BseX3I CGGCCG 1 cut(s) 994
BseXI GCAGC 3 cut(s) 512, 671, 958
Bsh1285I CGRYCG 1 cut(s) 997
BshFI GGCC 5 cut(s) 531, 747, 908, 954, 996
BshNI GGYRCC 1 cut(s) 209
BsiEI CGRYCG 1 cut(s) 997
BsiHKCI CYCGRG 1 cut(s) 1029
BsiSI CCGG 7 cut(s) 63, 237, 282, 327, 748, 905, 993
BslI CCNNNNNNNGG 3 cut(s) 251, 476, 1003
BsmAI GTCTC 2 cut(s) 476, 775
BsmBI CGTCTC 1 cut(s) 476
BsnI GGCC 5 cut(s) 531, 747, 908, 954, 996
Bso31I GGTCTC 1 cut(s) 775
BsoBI CYCGRG 1 cut(s) 1029
Bsp1286I GDGCHC 1 cut(s) 987
Bsp13I TCCGGA 1 cut(s) 281
Bsp143I GATC 4 cut(s) 24, 286, 592, 822
Bsp19I CCATGG 1 cut(s) 742
BspACI CCGC 6 cut(s) 5, 96, 99, 187, 512, 997
BspANI GGCC 5 cut(s) 531, 747, 908, 954, 996
BspEI TCCGGA 1 cut(s) 281
BspLI GGNNCC 2 cut(s) 211, 740
BspMI ACCTGC 2 cut(s) 28, 1003
BspPI GGATC 1 cut(s) 281
BspQI GCTCTTC 1 cut(s) 636
BspT107I GGYRCC 1 cut(s) 209
BspTNI GGTCTC 1 cut(s) 775
BsrDI GCAATG 1 cut(s) 1006
BsrFI RCCGGY 3 cut(s) 62, 904, 992
BsrI ACTGG 2 cut(s) 213, 415
BssAI RCCGGY 3 cut(s) 62, 904, 992
BssECI CCNNGG 1 cut(s) 742
BssMI GATC 4 cut(s) 24, 286, 592, 822
BssNI GRCGYC 1 cut(s) 210
BssT1I CCWWGG 1 cut(s) 742
Bst2UI CCWGG 2 cut(s) 207, 429
Bst4CI ACNGT 2 cut(s) 344, 487
Bst6I CTCTTC 2 cut(s) 330, 636
BstACI GRCGYC 1 cut(s) 210
BstC8I GCNNGC 3 cut(s) 842, 906, 994
BstDEI CTNAG 1 cut(s) 471
BstDSI CCRYGG 1 cut(s) 742
BstF5I GGATG 2 cut(s) 661, 771
BstH2I RGCGCY 2 cut(s) 213, 966
BstHHI GCGC 2 cut(s) 212, 965
BstKTI GATC 4 cut(s) 27, 289, 595, 825
BstMAI GTCTC 2 cut(s) 476, 775
BstMBI GATC 4 cut(s) 24, 286, 592, 822
BstMCI CGRYCG 1 cut(s) 997
BstMWI GCNNNNNNNGC 3 cut(s) 509, 960, 971
BstNI CCWGG 2 cut(s) 207, 429
BstNSI RCATGY 2 cut(s) 88, 844
BstSCI CCNGG 4 cut(s) 205, 235, 326, 427
BstSFI CTRYAG 1 cut(s) 246
BstV1I GCAGC 3 cut(s) 512, 671, 958
BstV2I GAAGAC 1 cut(s) 481
BstX2I RGATCY 1 cut(s) 286
BstXI CCANNNNNNTGG 2 cut(s) 220, 274
BstYI RGATCY 1 cut(s) 286
BstZI CGGCCG 1 cut(s) 994
BsuRI GGCC 5 cut(s) 531, 747, 908, 954, 996
BtgI CCRYGG 1 cut(s) 742
BtsCI GGATG 2 cut(s) 661, 771
BtsI GCAGTG 2 cut(s) 351, 726
BtsIMutI CAGTG 4 cut(s) 349, 351, 384, 726
BveI ACCTGC 2 cut(s) 28, 1003
Cac8I GCNNGC 3 cut(s) 842, 906, 994
CaiI CAGNNNCTG 1 cut(s) 506
CfoI GCGC 2 cut(s) 212, 965
Cfr10I RCCGGY 3 cut(s) 62, 904, 992
Csp6I GTAC 2 cut(s) 12, 340
CviAII CATG 5 cut(s) 85, 190, 733, 743, 841
CviQI GTAC 2 cut(s) 12, 340
DdeI CTNAG 1 cut(s) 471
DinI GGCGCC 1 cut(s) 211
DpnI GATC 4 cut(s) 26, 288, 594, 824
DpnII GATC 4 cut(s) 24, 286, 592, 822
DraI TTTAAA 1 cut(s) 666
EaeI YGGCCR 4 cut(s) 529, 745, 906, 994
EagI CGGCCG 1 cut(s) 994
Eam1104I CTCTTC 2 cut(s) 330, 636
EarI CTCTTC 2 cut(s) 330, 636
EclXI CGGCCG 1 cut(s) 994
Eco130I CCWWGG 1 cut(s) 742
Eco147I AGGCCT 1 cut(s) 954
Eco24I GRGCYC 1 cut(s) 987
Eco31I GGTCTC 1 cut(s) 775
Eco47III AGCGCT 1 cut(s) 964
Eco52I CGGCCG 1 cut(s) 994
Eco57I CTGAAG 2 cut(s) 620, 816
Eco88I CYCGRG 1 cut(s) 1029
EcoRII CCWGG 2 cut(s) 205, 427
EcoT14I CCWWGG 1 cut(s) 742
EcoT38I GRGCYC 1 cut(s) 987
EgeI GGCGCC 1 cut(s) 211
EheI GGCGCC 1 cut(s) 211
ErhI CCWWGG 1 cut(s) 742
Esp3I CGTCTC 1 cut(s) 476
FaeI CATG 5 cut(s) 88, 193, 736, 746, 844
FatI CATG 5 cut(s) 84, 189, 732, 742, 840
FbaI TGATCA 2 cut(s) 24, 822
Fnu4HI GCNGC 6 cut(s) 6, 99, 501, 660, 972, 997
FokI GGATG 2 cut(s) 668, 778
FriOI GRGCYC 1 cut(s) 987
Fsp4HI GCNGC 6 cut(s) 6, 99, 501, 660, 972, 997
FspBI CTAG 1 cut(s) 861
GlaI GCGC 2 cut(s) 211, 964
GluI GCNGC 6 cut(s) 6, 99, 501, 660, 972, 997
GsuI CTGGAG 1 cut(s) 951
HaeII RGCGCY 2 cut(s) 213, 966
HaeIII GGCC 5 cut(s) 531, 747, 908, 954, 996
HapII CCGG 7 cut(s) 63, 237, 282, 327, 748, 905, 993
HhaI GCGC 2 cut(s) 212, 965
Hin1I GRCGYC 1 cut(s) 210
Hin1II CATG 5 cut(s) 88, 193, 736, 746, 844
Hin6I GCGC 2 cut(s) 210, 963
HinP1I GCGC 2 cut(s) 210, 963
HindIII AAGCTT 2 cut(s) 116, 624
HinfI GANTC 6 cut(s) 77, 224, 278, 362, 376, 835
HpaII CCGG 7 cut(s) 63, 237, 282, 327, 748, 905, 993
HphI GGTGA 2 cut(s) 85, 157
Hpy166II GTNNAC 1 cut(s) 203
Hpy188I TCNGA 4 cut(s) 82, 254, 822, 978
Hpy188III TCNNGA 4 cut(s) 122, 282, 890, 1029
Hpy8I GTNNAC 1 cut(s) 203
HpyAV CCTTC 6 cut(s) 119, 238, 469, 791, 911, 944
HpyCH4III ACNGT 2 cut(s) 344, 487
HpyCH4IV ACGT 1 cut(s) 695
HpyCH4V TGCA 8 cut(s) 88, 356, 465, 539, 659, 719, 755, 844
HpyF10VI GCNNNNNNNGC 3 cut(s) 509, 960, 971
HpyF3I CTNAG 1 cut(s) 471
HpySE526I ACGT 1 cut(s) 695
Hsp92I GRCGYC 1 cut(s) 210
Hsp92II CATG 5 cut(s) 88, 193, 736, 746, 844
HspAI GCGC 2 cut(s) 210, 963
KasI GGCGCC 1 cut(s) 209
Kpn2I TCCGGA 1 cut(s) 281
KroI GCCGGC 2 cut(s) 904, 992
KroNI GCCGGC 2 cut(s) 906, 994
Ksp22I TGATCA 2 cut(s) 24, 822
Kzo9I GATC 4 cut(s) 24, 286, 592, 822
LguI GCTCTTC 1 cut(s) 636
LmnI GCTCC 2 cut(s) 744, 970
Lsp1109I GCAGC 3 cut(s) 512, 671, 958
LweI GCATC 4 cut(s) 512, 646, 682, 706
MaeI CTAG 1 cut(s) 861
MaeII ACGT 1 cut(s) 695
MaeIII GTNAC 3 cut(s) 58, 803, 863
MalI GATC 4 cut(s) 26, 288, 594, 824
MboI GATC 4 cut(s) 24, 286, 592, 822
MboII GAAGA 7 cut(s) 160, 347, 481, 613, 653, 934, 950
MflI RGATCY 1 cut(s) 286
MhlI GDGCHC 1 cut(s) 987
MlsI TGGCCA 1 cut(s) 531
MluCI AATT 3 cut(s) 533, 631, 808
MluNI TGGCCA 1 cut(s) 531
Mly113I GGCGCC 1 cut(s) 210
MlyI GAGTC 1 cut(s) 371
MmeI TCCRAC 2 cut(s) 232, 544
MnlI CCTC 5 cut(s) 10, 164, 405, 451, 729
Mox20I TGGCCA 1 cut(s) 531
MroI TCCGGA 1 cut(s) 281
MroNI GCCGGC 2 cut(s) 904, 992
MroXI GAANNNNTTC 1 cut(s) 897
MscI TGGCCA 1 cut(s) 531
MseI TTAA 5 cut(s) 297, 372, 665, 812, 828
MslI CAYNNNNRTG 2 cut(s) 387, 389
Msp20I TGGCCA 1 cut(s) 531
MspA1I CMGCKG 1 cut(s) 971
MspI CCGG 7 cut(s) 63, 237, 282, 327, 748, 905, 993
MspR9I CCNGG 4 cut(s) 207, 237, 328, 429
MvaI CCWGG 2 cut(s) 207, 429
MwoI GCNNNNNNNGC 3 cut(s) 509, 960, 971
NaeI GCCGGC 2 cut(s) 906, 994
NarI GGCGCC 1 cut(s) 210
NciI CCSGG 2 cut(s) 237, 328
NcoI CCATGG 1 cut(s) 742
NdeII GATC 4 cut(s) 24, 286, 592, 822
NgoMIV GCCGGC 2 cut(s) 904, 992
NlaIII CATG 5 cut(s) 88, 193, 736, 746, 844
NlaIV GGNNCC 2 cut(s) 211, 740
NmuCI GTSAC 2 cut(s) 58, 803
NspI RCATGY 2 cut(s) 88, 844
OliI CACNNNNGTG 1 cut(s) 389
PaeI GCATGC 1 cut(s) 844
PaqCI CACCTGC 2 cut(s) 28, 1003
PceI AGGCCT 1 cut(s) 954
PciSI GCTCTTC 1 cut(s) 636
PdiI GCCGGC 2 cut(s) 906, 994
PdmI GAANNNNTTC 1 cut(s) 897
PfeI GAWTC 5 cut(s) 77, 224, 278, 376, 835
PkrI GCNGC 6 cut(s) 7, 100, 502, 661, 973, 998
PleI GAGTC 1 cut(s) 370
PluTI GGCGCC 1 cut(s) 213
PpsI GAGTC 1 cut(s) 370
PsiI TTATAA 1 cut(s) 636
Psp1406I AACGTT 1 cut(s) 695
Psp6I CCWGG 2 cut(s) 205, 427
PspGI CCWGG 2 cut(s) 205, 427
PspN4I GGNNCC 2 cut(s) 211, 740
PstNI CAGNNNCTG 1 cut(s) 506
PsuI RGATCY 1 cut(s) 286
PvuII CAGCTG 1 cut(s) 971
RsaI GTAC 2 cut(s) 13, 341
RsaNI GTAC 2 cut(s) 12, 340
RseI CAYNNNNRTG 2 cut(s) 387, 389
SapI GCTCTTC 1 cut(s) 636
SaqAI TTAA 5 cut(s) 297, 372, 665, 812, 828
SatI GCNGC 6 cut(s) 6, 99, 501, 660, 972, 997
Sau3AI GATC 4 cut(s) 24, 286, 592, 822
SchI GAGTC 1 cut(s) 371
ScrFI CCNGG 4 cut(s) 207, 237, 328, 429
SduI GDGCHC 1 cut(s) 987
SfaNI GCATC 4 cut(s) 512, 646, 682, 706
SfcI CTRYAG 1 cut(s) 246
SfoI GGCGCC 1 cut(s) 211
SmiMI CAYNNNNRTG 2 cut(s) 387, 389
SmlI CTYRAG 2 cut(s) 113, 956
SmoI CTYRAG 2 cut(s) 113, 956
SphI GCATGC 1 cut(s) 844
Sse9I AATT 3 cut(s) 533, 631, 808
SseBI AGGCCT 1 cut(s) 954
SsiI CCGC 6 cut(s) 5, 96, 99, 187, 512, 997
SspDI GGCGCC 1 cut(s) 209
SspMI CTAG 1 cut(s) 861
StuI AGGCCT 1 cut(s) 954
StyD4I CCNGG 4 cut(s) 205, 235, 326, 427
StyI CCWWGG 1 cut(s) 742
TaaI ACNGT 2 cut(s) 344, 487
TaiI ACGT 1 cut(s) 698
TaqI TCGA 1 cut(s) 676
TasI AATT 3 cut(s) 533, 631, 808
TatI WGTACW 2 cut(s) 11, 339
TauI GCSGC 3 cut(s) 8, 101, 999
TfiI GAWTC 5 cut(s) 77, 224, 278, 376, 835
Tru1I TTAA 5 cut(s) 297, 372, 665, 812, 828
Tru9I TTAA 5 cut(s) 297, 372, 665, 812, 828
TscAI CASTG 4 cut(s) 349, 358, 391, 726
TseFI GTSAC 2 cut(s) 58, 803
TseI GCWGC 3 cut(s) 500, 659, 971
Tsp45I GTSAC 2 cut(s) 58, 803
TspDTI ATGAA 1 cut(s) 301
TspGWI ACGGA 2 cut(s) 374, 894
TspRI CASTG 4 cut(s) 349, 358, 391, 726
XceI RCATGY 2 cut(s) 88, 844
XmnI GAANNNNTTC 1 cut(s) 897
XspI CTAG 1 cut(s) 861
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.