Rw2G000760

2-alkenal reductase (NADP( )-dependent)-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000196
Physical Location & Seq
Reverse (-)
636220 .. 651794
15575 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G000760.1

Sequence Viewer

Length: 840 bp
ATGGCGGAAATGAAGAACAAGCAGGTGATACTGAAGGAATATGTGCCGGCCGGACTCCCCAAAGAATCTGACATGGCCGTCGTCACTACCACCACCAAACTGAAGCTTCCAGAAGGCTCCGCCGGGGTTCTGGTGAAGAACCTCTACTTGTCCTGCGATCCTTACATGAGAAGCCGCATGACCAAGCGCGACTCTGCTTCTTATGTCGATCCCTTCAACCCCGGCGAGTTTAAGCAAGGCGACTTGGTTTGGGGAATGATTGGCTGGGAAGAGTATAGTCTCATCACCTCCATTCAGTATTTGTTTAAGATTCCCACCACCGATGTGCCACTGTCTTATTATACTGGAATTCTTGGAGCGGTACGTCAGCTTGTTGGCCAATTTGCCTATACCTATTGTATACATGATATAGACATTTATACTATGACTCATGTTGTACATGTGAATCATATATACTACAACAAGTTTGGCTTTGACGGAGCCTTCAATTATAAAGGAGAACCTAACTTGGATGCAGCTTTGAAAAGGTACTTTCCTGAAGGAATTGATATATACTTTGAAAATGTTGGGGGAAATATGCTTGATGCAGTGCTGCTGAACATGAAGATTGGTGGCCGAATCTCAGTTTGTGTACAGAATTTAATGTATCCAGTGTCTAAACAGCTGCGCATGGAAGGCTTGCTGGTTACTAAATACTTTCCTCTCTACGGAAAGTTTCTGGAATTGGTTCTGCCTTACATAAAAGAAGGGAAGATTACCTACAATGCTGCATCGGCTCTGCTTGGGCTCTTCGCTGGCCGCAATGTAGGAAAGCAGGTGGTTGTTGTTTCCCGAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006950 GO:0006979 GO:0008150 GO:0016020 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N_2 ADH_zinc_N

Protein Analysis

279

Amino Acids

31.47

Weight (kDa)

8.52

Isoelectric Point (pI)

31.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N_2 PF16884 7 - 76 1.8e-11 N-terminal domain of oxidoreductase
ADH_zinc_N PF00107 152 - 210 8.1e-07 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000196)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65560
fragaria_vesca FvH4_1g00330 FvH4_1g00342 FvH4_1g00343 FvH4_1g00344 FvH4_1g00345 FvH4_1g00345 FvH4_1g00346 FvH4_1g26520
malus_domestica MD02G1001800.v1.1 MD02G1001900.v1.1 MD02G1002100.v1.1 MD02G1002300.v1.1 MD02G1002400.v1.1 MD02G1002500.v1.1 MD02G1002600.v1.1 MD15G1145700.v1.1
prunus_persica Prupe.7G268600_v2.0.a1 Prupe.7G268700_v2.0.a1 Prupe.7G268800_v2.0.a1 Prupe.7G268800_v2.0.a1 Prupe.7G268900_v2.0.a1
pyrus_communis pycom02g00050 pycom02g00060 pycom02g00100 pycom02g00120 pycom02g00130 pycom15g13090 pycom15g13100
rosa_chinensis RchiOBHm_Chr1g0325321 RchiOBHm_Chr1g0325341 RchiOBHm_Chr1g0325351 RchiOBHm_Chr1g0325361 RchiOBHm_Chr1g0364881 RchiOBHm_Chr1g0364891 RchiOBHm_Chr2g0084701 RchiOBHm_Chr2g0084721 RchiOBHm_Chr2g0084731 RchiOBHm_Chr2g0084741 RchiOBHm_Chr2g0084751 RchiOBHm_Chr2g0084761 RchiOBHm_Chr2g0084771 RchiOBHm_Chr2g0085311 RchiOBHm_Chr2g0085321 RchiOBHm_Chr2g0085341 RchiOBHm_Chr2g0085351 RchiOBHm_Chr2g0117721 RchiOBHm_Chr2g0159631 RchiOBHm_Chr3g0484011 RchiOBHm_Chr5g0021141 RchiOBHm_Chr5g0061081 RchiOBHm_Chr6g0275731 RchiOBHm_Chr7g0238011
rosa_laevigata RLG00000013438 RLG00000015632 RLG00000015634 RLG00000015635 RLG00000015636 RLG00000015637 RLG00000015677 RLG00000015679 RLG00000030145 RLG00000030146 RLG00000035427
rosa_multiflora Rmu_co8334849.1_g000001 Rmu_sc0001366.1_g000035 Rmu_sc0002352.1_g000003 Rmu_sc0002352.1_g000004 Rmu_sc0002352.1_g000006 Rmu_sc0002352.1_g000007 Rmu_sc0002352.1_g000009 Rmu_sc0002586.1_g000008 Rmu_sc0004966.1_g000016 Rmu_sc0007122.1_g000001 Rmu_sc0008804.1_g000002 Rmu_sc0008883.1_g000001 Rmu_sc0010860.1_g000002 Rmu_sc0012119.1_g000001 Rmu_sc0012119.1_g000003 Rmu_sc0012119.1_g000006 Rmu_sc0012119.1_g000007 Rmu_sc0012119.1_g000009 Rmu_sc0020031.1_g000004 Rmu_sc0020800.1_g000005 Rmu_sc0020800.1_g000007 Rmu_sc0020800.1_g000008 Rmu_sc0020800.1_g000009 Rmu_sc0025597.1_g000001 Rmu_sc0029132.1_g000002
rosa_roxburghii Rroxscaffold_2G00155450 Rroxscaffold_2G00155460 Rroxscaffold_2G00155470 Rroxscaffold_2G00155830 Rroxscaffold_2G00155840 Rroxscaffold_2G00155850 Rroxscaffold_2G00155860 Rroxscaffold_2G00155870 Rroxscaffold_2G00155910 Rroxscaffold_3G00273850 Rroxscaffold_4G00324820 Rroxscaffold_7G00192930 Rroxscaffold_7G00192960
rosa_rugosa Rorug01G0051300 Rorug01G0051400 Rorug01G0455700 Rorug01G0455800 Rorug01G0455900 Rorug01G0459500 Rorug01G0459500 Rorug01G0459600 Rorug01G0459700 Rorug01G0459700 Rorug02G0335000
rosa_samantha Rh1AG066500 Rh1BG054700 Rh1BG054800 Rh1BG176600 Rh2BG004400 Rh2BG004500 Rh2BG004600 Rh2BG004700 Rh2BG008600 Rh2BG008700 Rh2CG004500 Rh2CG004700 Rh2CG004800 Rh2CG004900 Rh2CG005000 Rh2CG005100 Rh2CG005200 Rh2CG009300 Rh2CG009400 Rh2DG004200 Rh2DG004300 Rh2DG004400 Rh2DG004500 Rh2DG004600 Rh2DG009800 Rh5BG411800 Rh5CG167200 Rh5CG436800 Rh5CG578800 Rh6DG203800
rosa_wichuraiana Rw1G005560 Rw2G000320 Rw2G000350 Rw2G000360 Rw2G000370 Rw2G000750 Rw2G000760 Rw4G022130 Rw5G015810 Rw5G037620 Rw6G018120 Rw7G038530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 492
AarI CACCTGC 2 cut(s) 13, 805
AasI GACNNNNNNGTC 1 cut(s) 77
Acc16I TGCGCA 1 cut(s) 668
Acc36I ACCTGC 2 cut(s) 13, 805
AccBSI CCGCTC 1 cut(s) 359
AccI GTMKAC 1 cut(s) 400
AccII CGCG 1 cut(s) 189
AciI CCGC 5 cut(s) 5, 120, 175, 359, 799
AclWI GGATC 2 cut(s) 152, 203
AcoI YGGCCR 5 cut(s) 48, 75, 376, 613, 796
AcsI RAATTY 2 cut(s) 348, 637
AcuI CTGAAG 3 cut(s) 53, 122, 558
AfaI GTAC 4 cut(s) 363, 438, 530, 633
AfiI CCNNNNNNNGG 1 cut(s) 707
AflIII ACRYGT 1 cut(s) 439
AgsI TTSAA 4 cut(s) 217, 487, 523, 560
AleI CACNNNNGTG 1 cut(s) 323
AluBI AGCT 4 cut(s) 106, 370, 518, 664
AluI AGCT 4 cut(s) 106, 370, 518, 664
Alw26I GTCTC 1 cut(s) 284
AlwI GGATC 2 cut(s) 152, 203
Ama87I CYCGRG 1 cut(s) 831
AoxI GGCC 5 cut(s) 48, 75, 376, 613, 796
ApeKI GCWGC 4 cut(s) 515, 592, 664, 767
ApoI RAATTY 2 cut(s) 348, 637
Asp700I GAANNNNTTC 1 cut(s) 726
AspLEI GCGC 2 cut(s) 189, 669
AsuC2I CCSGG 2 cut(s) 124, 222
AsuHPI GGTGA 3 cut(s) 37, 145, 277
AvaI CYCGRG 1 cut(s) 831
BalI TGGCCA 1 cut(s) 378
BanII GRGCYC 1 cut(s) 789
BarI GAAGNNNNNNTAC 4 cut(s) 128, 160, 743, 775
BbvI GCAGC 4 cut(s) 527, 579, 651, 754
BceAI ACGGC 1 cut(s) 62
BciVI GTATCC 1 cut(s) 657
BcnI CCSGG 2 cut(s) 124, 222
BcoDI GTCTC 1 cut(s) 284
BfuAI ACCTGC 2 cut(s) 13, 805
BfuI GTATCC 1 cut(s) 657
BisI GCNGC 6 cut(s) 175, 516, 593, 665, 768, 799
BlsI GCNGC 6 cut(s) 176, 517, 594, 666, 769, 800
Bme1390I CCNGG 2 cut(s) 124, 222
BmeT110I CYCGRG 1 cut(s) 831
BmiI GGNNCC 2 cut(s) 118, 481
BmrFI CCNGG 2 cut(s) 124, 222
BmsI GCATC 3 cut(s) 502, 574, 779
BpuMI CCSGG 2 cut(s) 124, 222
BsaJI CCNNGG 2 cut(s) 123, 220
Bsc4I CCNNNNNNNGG 1 cut(s) 707
Bse118I RCCGGY 1 cut(s) 46
Bse1I ACTGG 2 cut(s) 349, 650
Bse3DI GCAATG 1 cut(s) 808
BseDI CCNNGG 2 cut(s) 123, 220
BseGI GGATG 1 cut(s) 517
BseLI CCNNNNNNNGG 1 cut(s) 707
BseMI GCAATG 1 cut(s) 808
BseMII CTCAG 1 cut(s) 636
BseNI ACTGG 2 cut(s) 349, 650
BseX3I CGGCCG 1 cut(s) 48
BseXI GCAGC 4 cut(s) 527, 579, 651, 754
BseYI CCCAGC 1 cut(s) 264
Bsh1236I CGCG 1 cut(s) 189
Bsh1285I CGRYCG 1 cut(s) 51
BshFI GGCC 5 cut(s) 50, 77, 378, 615, 798
BsiEI CGRYCG 1 cut(s) 51
BsiHKCI CYCGRG 1 cut(s) 831
BsiSI CCGG 4 cut(s) 47, 51, 123, 222
BslI CCNNNNNNNGG 1 cut(s) 707
BsmAI GTCTC 1 cut(s) 284
BsnI GGCC 5 cut(s) 50, 77, 378, 615, 798
BsoBI CYCGRG 1 cut(s) 831
Bsp1286I GDGCHC 1 cut(s) 789
Bsp1407I TGTACA 2 cut(s) 436, 631
Bsp143I GATC 2 cut(s) 157, 208
BspACI CCGC 5 cut(s) 5, 120, 175, 359, 799
BspANI GGCC 5 cut(s) 50, 77, 378, 615, 798
BspCNI CTCAG 1 cut(s) 635
BspFNI CGCG 1 cut(s) 189
BspLI GGNNCC 2 cut(s) 118, 481
BspMI ACCTGC 2 cut(s) 13, 805
BspPI GGATC 2 cut(s) 152, 203
BspQI GCTCTTC 1 cut(s) 794
BsrBI CCGCTC 1 cut(s) 359
BsrDI GCAATG 1 cut(s) 808
BsrFI RCCGGY 1 cut(s) 46
BsrGI TGTACA 2 cut(s) 436, 631
BsrI ACTGG 2 cut(s) 349, 650
BssAI RCCGGY 1 cut(s) 46
BssECI CCNNGG 2 cut(s) 123, 220
BssMI GATC 2 cut(s) 157, 208
BssNAI GTATAC 1 cut(s) 401
Bst1107I GTATAC 1 cut(s) 401
Bst4CI ACNGT 1 cut(s) 333
Bst6I CTCTTC 2 cut(s) 264, 794
BstAUI TGTACA 2 cut(s) 436, 631
BstC8I GCNNGC 3 cut(s) 48, 680, 796
BstDEI CTNAG 1 cut(s) 622
BstF5I GGATG 1 cut(s) 517
BstFNI CGCG 1 cut(s) 189
BstHHI GCGC 2 cut(s) 189, 669
BstKTI GATC 2 cut(s) 160, 211
BstMAI GTCTC 1 cut(s) 284
BstMBI GATC 2 cut(s) 157, 208
BstMCI CGRYCG 1 cut(s) 51
BstMWI GCNNNNNNNGC 2 cut(s) 675, 773
BstNSI RCATGY 1 cut(s) 443
BstSCI CCNGG 2 cut(s) 122, 220
BstUI CGCG 1 cut(s) 189
BstV1I GCAGC 4 cut(s) 527, 579, 651, 754
BstZ17I GTATAC 1 cut(s) 401
BstZI CGGCCG 1 cut(s) 48
BsuI GTATCC 1 cut(s) 657
BsuRI GGCC 5 cut(s) 50, 77, 378, 615, 798
BtsCI GGATG 1 cut(s) 517
BtsI GCAGTG 1 cut(s) 594
BtsIMutI CAGTG 3 cut(s) 329, 594, 657
BveI ACCTGC 2 cut(s) 13, 805
Cac8I GCNNGC 3 cut(s) 48, 680, 796
CfoI GCGC 2 cut(s) 189, 669
Cfr10I RCCGGY 1 cut(s) 46
Csp6I GTAC 4 cut(s) 362, 437, 529, 632
CviAII CATG 8 cut(s) 73, 166, 178, 404, 431, 440, 601, 670
CviQI GTAC 4 cut(s) 362, 437, 529, 632
DdeI CTNAG 1 cut(s) 622
DpnI GATC 2 cut(s) 159, 210
DpnII GATC 2 cut(s) 157, 208
DrdI GACNNNNNNGTC 1 cut(s) 77
DseDI GACNNNNNNGTC 1 cut(s) 77
EaeI YGGCCR 5 cut(s) 48, 75, 376, 613, 796
EagI CGGCCG 1 cut(s) 48
Eam1104I CTCTTC 2 cut(s) 264, 794
EarI CTCTTC 2 cut(s) 264, 794
EciI GGCGGA 2 cut(s) 20, 109
EclXI CGGCCG 1 cut(s) 48
Eco24I GRGCYC 1 cut(s) 789
Eco52I CGGCCG 1 cut(s) 48
Eco57I CTGAAG 3 cut(s) 53, 122, 558
Eco88I CYCGRG 1 cut(s) 831
EcoRI GAATTC 1 cut(s) 348
EcoT38I GRGCYC 1 cut(s) 789
FaeI CATG 8 cut(s) 76, 169, 181, 407, 434, 443, 604, 673
FalI AAGNNNNNCTT 2 cut(s) 455, 487
FatI CATG 8 cut(s) 72, 165, 177, 403, 430, 439, 600, 669
FblI GTMKAC 1 cut(s) 400
Fnu4HI GCNGC 6 cut(s) 175, 516, 593, 665, 768, 799
FokI GGATG 1 cut(s) 524
FriOI GRGCYC 1 cut(s) 789
Fsp4HI GCNGC 6 cut(s) 175, 516, 593, 665, 768, 799
FspI TGCGCA 1 cut(s) 668
GlaI GCGC 2 cut(s) 188, 668
GluI GCNGC 6 cut(s) 175, 516, 593, 665, 768, 799
GsaI CCCAGC 1 cut(s) 268
HaeIII GGCC 5 cut(s) 50, 77, 378, 615, 798
HapII CCGG 4 cut(s) 47, 51, 123, 222
HhaI GCGC 2 cut(s) 189, 669
Hin1II CATG 8 cut(s) 76, 169, 181, 407, 434, 443, 604, 673
Hin6I GCGC 2 cut(s) 187, 667
HinP1I GCGC 2 cut(s) 187, 667
HindIII AAGCTT 1 cut(s) 104
HinfI GANTC 7 cut(s) 54, 65, 191, 310, 427, 445, 618
HpaII CCGG 4 cut(s) 47, 51, 123, 222
HphI GGTGA 3 cut(s) 37, 145, 277
Hpy166II GTNNAC 2 cut(s) 401, 632
Hpy188I TCNGA 1 cut(s) 70
Hpy188III TCNNGA 4 cut(s) 110, 536, 719, 831
Hpy8I GTNNAC 2 cut(s) 401, 632
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 7 cut(s) 28, 107, 223, 493, 533, 668, 740
HpyCH4III ACNGT 1 cut(s) 333
HpyCH4IV ACGT 1 cut(s) 364
HpyCH4V TGCA 3 cut(s) 515, 587, 770
HpyF10VI GCNNNNNNNGC 2 cut(s) 675, 773
HpyF3I CTNAG 1 cut(s) 622
HpySE526I ACGT 1 cut(s) 364
Hsp92II CATG 8 cut(s) 76, 169, 181, 407, 434, 443, 604, 673
HspAI GCGC 2 cut(s) 187, 667
KroI GCCGGC 1 cut(s) 46
KroNI GCCGGC 1 cut(s) 48
Kzo9I GATC 2 cut(s) 157, 208
LguI GCTCTTC 1 cut(s) 794
LmnI GCTCC 3 cut(s) 122, 356, 479
Lsp1109I GCAGC 4 cut(s) 527, 579, 651, 754
LweI GCATC 3 cut(s) 502, 574, 779
MaeII ACGT 1 cut(s) 364
MaeIII GTNAC 2 cut(s) 82, 685
MalI GATC 2 cut(s) 159, 210
MbiI CCGCTC 1 cut(s) 359
MboI GATC 2 cut(s) 157, 208
MboII GAAGA 6 cut(s) 25, 148, 281, 616, 763, 781
MhlI GDGCHC 1 cut(s) 789
MlsI TGGCCA 1 cut(s) 378
MluCI AATT 6 cut(s) 348, 380, 487, 543, 637, 722
MluNI TGGCCA 1 cut(s) 378
MlyI GAGTC 3 cut(s) 48, 185, 421
MnlI CCTC 3 cut(s) 152, 298, 711
Mox20I TGGCCA 1 cut(s) 378
MroNI GCCGGC 1 cut(s) 46
MroXI GAANNNNTTC 1 cut(s) 726
MscI TGGCCA 1 cut(s) 378
MseI TTAA 3 cut(s) 231, 306, 641
MslI CAYNNNNRTG 1 cut(s) 323
Msp20I TGGCCA 1 cut(s) 378
MspA1I CMGCKG 1 cut(s) 664
MspI CCGG 4 cut(s) 47, 51, 123, 222
MspR9I CCNGG 2 cut(s) 124, 222
MvnI CGCG 1 cut(s) 189
MwoI GCNNNNNNNGC 2 cut(s) 675, 773
NaeI GCCGGC 1 cut(s) 48
NciI CCSGG 2 cut(s) 124, 222
NdeII GATC 2 cut(s) 157, 208
NgoMIV GCCGGC 1 cut(s) 46
NlaIII CATG 8 cut(s) 76, 169, 181, 407, 434, 443, 604, 673
NlaIV GGNNCC 2 cut(s) 118, 481
NmuCI GTSAC 1 cut(s) 82
NsbI TGCGCA 1 cut(s) 668
NspI RCATGY 1 cut(s) 443
OliI CACNNNNGTG 1 cut(s) 323
PaqCI CACCTGC 2 cut(s) 13, 805
PciI ACATGT 1 cut(s) 439
PciSI GCTCTTC 1 cut(s) 794
PdiI GCCGGC 1 cut(s) 48
PdmI GAANNNNTTC 1 cut(s) 726
PfeI GAWTC 4 cut(s) 65, 310, 445, 618
PkrI GCNGC 6 cut(s) 176, 517, 594, 666, 769, 800
PleI GAGTC 3 cut(s) 48, 185, 421
PpsI GAGTC 3 cut(s) 48, 185, 421
PscI ACATGT 1 cut(s) 439
PsiI TTATAA 1 cut(s) 492
PspFI CCCAGC 1 cut(s) 264
PspN4I GGNNCC 2 cut(s) 118, 481
PvuII CAGCTG 1 cut(s) 664
RsaI GTAC 4 cut(s) 363, 438, 530, 633
RsaNI GTAC 4 cut(s) 362, 437, 529, 632
RseI CAYNNNNRTG 1 cut(s) 323
SapI GCTCTTC 1 cut(s) 794
SaqAI TTAA 3 cut(s) 231, 306, 641
SatI GCNGC 6 cut(s) 175, 516, 593, 665, 768, 799
Sau3AI GATC 2 cut(s) 157, 208
SchI GAGTC 3 cut(s) 48, 185, 421
ScrFI CCNGG 2 cut(s) 124, 222
SduI GDGCHC 1 cut(s) 789
SfaNI GCATC 3 cut(s) 502, 574, 779
SmiMI CAYNNNNRTG 1 cut(s) 323
Sse9I AATT 6 cut(s) 348, 380, 487, 543, 637, 722
SsiI CCGC 5 cut(s) 5, 120, 175, 359, 799
StyD4I CCNGG 2 cut(s) 122, 220
TaaI ACNGT 1 cut(s) 333
TaiI ACGT 1 cut(s) 367
TaqI TCGA 1 cut(s) 207
TasI AATT 6 cut(s) 348, 380, 487, 543, 637, 722
TatI WGTACW 2 cut(s) 436, 631
TauI GCSGC 2 cut(s) 177, 801
TfiI GAWTC 4 cut(s) 65, 310, 445, 618
Tru1I TTAA 3 cut(s) 231, 306, 641
Tru9I TTAA 3 cut(s) 231, 306, 641
TscAI CASTG 3 cut(s) 336, 594, 657
TseFI GTSAC 1 cut(s) 82
TseI GCWGC 4 cut(s) 515, 592, 664, 767
Tsp45I GTSAC 1 cut(s) 82
TspDTI ATGAA 2 cut(s) 26, 617
TspGWI ACGGA 2 cut(s) 492, 723
TspRI CASTG 3 cut(s) 336, 594, 657
XapI RAATTY 2 cut(s) 348, 637
XceI RCATGY 1 cut(s) 443
XmiI GTMKAC 1 cut(s) 400
XmnI GAANNNNTTC 1 cut(s) 726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.