Rorug01G0051400

2-alkenal reductase (NADP( )-dependent)-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000196
Physical Location & Seq
Reverse (-)
8361623 .. 8366483
4861 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0051400.1

Sequence Viewer

Length: 1698 bp
ATGACACTCGGAAACAACGGGCGTGAGTTACTCGTCAAGATGCTCTCCTCCGACCACCAAAAGACCCCCTCTCGTGGTGGCTGGAACGCCGCCATTTTCATCATCTTCGTCGAGGTTGCAGAGAGGTTTGCTTACTATGGCCTGATAGGTAACCTCATCATGTACCTCACCAATGAGCTCCACCAGCCCACCGCTGCCGCCGCCAAGAACGTCAACTTGTGGATCGGCCTCTCCTCCCTCTTCCCCGTCCTCGGGGCCATCGTCGCCGATTCTTACCTCGGCCGCTTCAAGACAATCATCTACTCCACCTGCATTTATTTCCTGGGAATGGTTATGCTGTGCTTATCTGCTTCGGTGGTTCCTCGGCCTTATCGTGAAGGGGCATACTTCGTGGGGCTTTACATTCTGGCGGTGGGAGAAGGCGGCCACAAGCCTTGCGTGCAGACCTTCGCCGCCGACCAGTTTGAGGAGGAGACTGATGAGGACAAGGAGGCTAAGAGCTCATTCTTCAACTGGTGGTATTTGGCTATAGTTGTCACCGCTACTGCTGCCACACTTGGTATCATCTATATTCAGGATAATGTGAGCTGGGTGCTGAGCTATAGTGTCTTGGCAGTGGTAATAGCAGCAGGCCTCCTATTATTTCTACTCGGAATCCGAAGGTATCGGAACGAGGATAATCTGTTGGGGAGCCCCTTCACGACGGTGGCGCAAGTATTTGTGGCGGCATCTTGGAAGTGGCGCGTAAAGACGGGTCGTCGGAATGATGTTGTCTACTACGGTGATGATGAGGGTGAGAGCAGTGGCGTTCACTCCAAGCACAAGACATTGGCTCGTACTAAACAATTCAGATTTCTGGACAAGGCAATGATTATTGACGAACATGATGCGTCAAGCAAAATGAGGAATCCTTGGAGGTTATGCTCTCTAAATCAAGTGGAAGAAGTGAAGCTTGTTGTCCGTCTAATTCCCATATGGTTGAGCTGCCTAATGTTTCACGCAGTTCAATCTCAACTCCACACCTACTTCATCAAACAAGGTTCCACGATGATCAGATCCATCGGACCCAACTTTCGGTTCCCCCCTGCATCACTTCAAGCCCTAGTTGGCATTGTGATCGTAACTACCGTTCCAATCTACGACCGGATTCTTGTCCCGACAGTAAGAAAGTTCACAGGACATCCCGCGGGCATAACTGTCCTCCAACGAATTGGTGTGGGCCTATTCTTGTCCATAATCAACATGGTTGTGGCAGCTCTAGTGGAAGCTAAAAGGGTTGGTGTTGCAAGAGAATATAACCTCATGGATAACCCAAAAGCAATGGTACCAATCAGTGTCTGGTGGTTACTCCCACAATACTTTATCACTGGTTTGTCTGATGCATTCACCGTTATAGGACTCCAAGAGTTGTTCTATGATCAAATGCCAGATGCAATGAGAAGCTTGGGAGCTGCAGCATACATTAGTATTCTTGGAGTTGGAAGCTTTCTTAGCACTTTCATCATATCCTTGGTGGAAACTATTACCAGAAACTCTGGTGAGCCGTGGTTAGGTAACAATCTCAACCGCGCACACCTTGATGACTTCTACTGGGTGCTTGCAGGACTAAGCGCTCTATTTTTCGGTGTTTATGTGTGGGTTGCAAAGCGATTTGTTTACAAGAAAGTTGTAGGGGAAGCATCAAGCGGGGAGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006950 GO:0006979 GO:0008150 GO:0016020 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N_2 ADH_zinc_N

Protein Analysis

565

Amino Acids

63.04

Weight (kDa)

8.84

Isoelectric Point (pI)

30.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 96 - 510 2.2e-91 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000196)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65560
fragaria_vesca FvH4_1g00330 FvH4_1g00342 FvH4_1g00343 FvH4_1g00344 FvH4_1g00345 FvH4_1g00345 FvH4_1g00346 FvH4_1g26520
malus_domestica MD02G1001800.v1.1 MD02G1001900.v1.1 MD02G1002100.v1.1 MD02G1002300.v1.1 MD02G1002400.v1.1 MD02G1002500.v1.1 MD02G1002600.v1.1 MD15G1145700.v1.1
prunus_persica Prupe.7G268600_v2.0.a1 Prupe.7G268700_v2.0.a1 Prupe.7G268800_v2.0.a1 Prupe.7G268800_v2.0.a1 Prupe.7G268900_v2.0.a1
pyrus_communis pycom02g00050 pycom02g00060 pycom02g00100 pycom02g00120 pycom02g00130 pycom15g13090 pycom15g13100
rosa_chinensis RchiOBHm_Chr1g0325321 RchiOBHm_Chr1g0325341 RchiOBHm_Chr1g0325351 RchiOBHm_Chr1g0325361 RchiOBHm_Chr1g0364881 RchiOBHm_Chr1g0364891 RchiOBHm_Chr2g0084701 RchiOBHm_Chr2g0084721 RchiOBHm_Chr2g0084731 RchiOBHm_Chr2g0084741 RchiOBHm_Chr2g0084751 RchiOBHm_Chr2g0084761 RchiOBHm_Chr2g0084771 RchiOBHm_Chr2g0085311 RchiOBHm_Chr2g0085321 RchiOBHm_Chr2g0085341 RchiOBHm_Chr2g0085351 RchiOBHm_Chr2g0117721 RchiOBHm_Chr2g0159631 RchiOBHm_Chr3g0484011 RchiOBHm_Chr5g0021141 RchiOBHm_Chr5g0061081 RchiOBHm_Chr6g0275731 RchiOBHm_Chr7g0238011
rosa_laevigata RLG00000013438 RLG00000015632 RLG00000015634 RLG00000015635 RLG00000015636 RLG00000015637 RLG00000015677 RLG00000015679 RLG00000030145 RLG00000030146 RLG00000035427
rosa_multiflora Rmu_co8334849.1_g000001 Rmu_sc0001366.1_g000035 Rmu_sc0002352.1_g000003 Rmu_sc0002352.1_g000004 Rmu_sc0002352.1_g000006 Rmu_sc0002352.1_g000007 Rmu_sc0002352.1_g000009 Rmu_sc0002586.1_g000008 Rmu_sc0004966.1_g000016 Rmu_sc0007122.1_g000001 Rmu_sc0008804.1_g000002 Rmu_sc0008883.1_g000001 Rmu_sc0010860.1_g000002 Rmu_sc0012119.1_g000001 Rmu_sc0012119.1_g000003 Rmu_sc0012119.1_g000006 Rmu_sc0012119.1_g000007 Rmu_sc0012119.1_g000009 Rmu_sc0020031.1_g000004 Rmu_sc0020800.1_g000005 Rmu_sc0020800.1_g000007 Rmu_sc0020800.1_g000008 Rmu_sc0020800.1_g000009 Rmu_sc0025597.1_g000001 Rmu_sc0029132.1_g000002
rosa_roxburghii Rroxscaffold_2G00155450 Rroxscaffold_2G00155460 Rroxscaffold_2G00155470 Rroxscaffold_2G00155830 Rroxscaffold_2G00155840 Rroxscaffold_2G00155850 Rroxscaffold_2G00155860 Rroxscaffold_2G00155870 Rroxscaffold_2G00155910 Rroxscaffold_3G00273850 Rroxscaffold_4G00324820 Rroxscaffold_7G00192930 Rroxscaffold_7G00192960
rosa_rugosa Rorug01G0051300 Rorug01G0051400 Rorug01G0455700 Rorug01G0455800 Rorug01G0455900 Rorug01G0459500 Rorug01G0459500 Rorug01G0459600 Rorug01G0459700 Rorug01G0459700 Rorug02G0335000
rosa_samantha Rh1AG066500 Rh1BG054700 Rh1BG054800 Rh1BG176600 Rh2BG004400 Rh2BG004500 Rh2BG004600 Rh2BG004700 Rh2BG008600 Rh2BG008700 Rh2CG004500 Rh2CG004700 Rh2CG004800 Rh2CG004900 Rh2CG005000 Rh2CG005100 Rh2CG005200 Rh2CG009300 Rh2CG009400 Rh2DG004200 Rh2DG004300 Rh2DG004400 Rh2DG004500 Rh2DG004600 Rh2DG009800 Rh5BG411800 Rh5CG167200 Rh5CG436800 Rh5CG578800 Rh6DG203800
rosa_wichuraiana Rw1G005560 Rw2G000320 Rw2G000350 Rw2G000360 Rw2G000370 Rw2G000750 Rw2G000760 Rw4G022130 Rw5G015810 Rw5G037620 Rw6G018120 Rw7G038530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 317
Acc36I ACCTGC 1 cut(s) 317
Acc65I GGTACC 1 cut(s) 1324
AccB1I GGYRCC 1 cut(s) 1324
AccI GTMKAC 1 cut(s) 774
AccII CGCG 3 cut(s) 744, 1187, 1569
AclWI GGATC 2 cut(s) 230, 1050
AcoI YGGCCR 2 cut(s) 280, 424
AfaI GTAC 3 cut(s) 164, 838, 1326
AfeI AGCGCT 1 cut(s) 1612
AfiI CCNNNNNNNGG 7 cut(s) 74, 251, 252, 328, 466, 1074, 1550
AgsI TTSAA 4 cut(s) 289, 511, 1007, 1097
AhdI GACNNNNNGTC 1 cut(s) 756
AjnI CCWGG 1 cut(s) 321
AjuI GAANNNNNNNTTGG 2 cut(s) 1061, 1093
AleI CACNNNNGTG 1 cut(s) 704
Alw21I GWGCWC 2 cut(s) 180, 503
Alw26I GTCTC 1 cut(s) 467
AlwI GGATC 2 cut(s) 230, 1050
AlwNI CAGNNNCTG 1 cut(s) 1338
Ama87I CYCGRG 1 cut(s) 251
Aor51HI AGCGCT 1 cut(s) 1612
AoxI GGCC 8 cut(s) 139, 226, 255, 280, 365, 424, 631, 1219
ApeKI GCWGC 7 cut(s) 194, 548, 626, 984, 1253, 1451, 1454
ArsI GACNNNNNNTTYG 2 cut(s) 1056, 1088
Asp718I GGTACC 1 cut(s) 1324
AspLEI GCGC 4 cut(s) 712, 744, 1571, 1613
AspS9I GGNCC 3 cut(s) 255, 1064, 1219
AsuHPI GGTGA 6 cut(s) 160, 529, 794, 806, 1378, 1550
AvaI CYCGRG 1 cut(s) 251
AvaII GGWCC 1 cut(s) 1064
BanI GGYRCC 1 cut(s) 1324
BanII GRGCYC 3 cut(s) 180, 503, 695
BauI CACGAG 1 cut(s) 72
Bbv12I GWGCWC 2 cut(s) 180, 503
BbvI GCAGC 7 cut(s) 181, 535, 638, 971, 1265, 1438, 1466
BccI CCATC 2 cut(s) 266, 1067
BceAI ACGGC 1 cut(s) 1528
BcgI CGANNNNNNTGC 4 cut(s) 869, 903, 1099, 1133
BciT130I CCWGG 1 cut(s) 323
BclI TGATCA 2 cut(s) 1050, 1417
BcoDI GTCTC 1 cut(s) 467
BfaI CTAG 2 cut(s) 1103, 1259
BfmI CTRYAG 3 cut(s) 528, 601, 1452
BfoI RGCGCY 1 cut(s) 1614
BfuAI ACCTGC 1 cut(s) 317
BlpI GCTNAGC 1 cut(s) 596
Bme1390I CCNGG 1 cut(s) 323
Bme18I GGWCC 1 cut(s) 1064
BmeRI GACNNNNNGTC 1 cut(s) 756
BmeT110I CYCGRG 1 cut(s) 251
BmgT120I GGNCC 3 cut(s) 255, 1064, 1219
BmiI GGNNCC 7 cut(s) 256, 360, 692, 1042, 1066, 1079, 1326
BmrFI CCNGG 1 cut(s) 323
BmrI ACTGGG 1 cut(s) 1600
BmsI GCATC 7 cut(s) 30, 737, 877, 1097, 1369, 1420, 1688
BmuI ACTGGG 1 cut(s) 1600
Bpu1102I GCTNAGC 1 cut(s) 596
BsaJI CCNNGG 8 cut(s) 250, 277, 322, 362, 911, 1185, 1509, 1544
BsaWI WCCGGW 1 cut(s) 1143
Bsc4I CCNNNNNNNGG 7 cut(s) 74, 251, 252, 328, 466, 1074, 1550
Bse1I ACTGG 4 cut(s) 460, 518, 1372, 1595
Bse3DI GCAATG 3 cut(s) 873, 1326, 1440
BseBI CCWGG 1 cut(s) 323
BseDI CCNNGG 8 cut(s) 250, 277, 322, 362, 911, 1185, 1509, 1544
BseGI GGATG 1 cut(s) 1180
BseLI CCNNNNNNNGG 7 cut(s) 74, 251, 252, 328, 466, 1074, 1550
BseMI GCAATG 3 cut(s) 873, 1326, 1440
BseMII CTCAG 1 cut(s) 587
BseNI ACTGG 4 cut(s) 460, 518, 1372, 1595
BseRI GAGGAG 4 cut(s) 37, 223, 482, 485
BseX3I CGGCCG 1 cut(s) 280
BseXI GCAGC 7 cut(s) 181, 535, 638, 971, 1265, 1438, 1466
BseYI CCCAGC 1 cut(s) 588
BsgI GTGCAG 1 cut(s) 461
Bsh1236I CGCG 3 cut(s) 744, 1187, 1569
Bsh1285I CGRYCG 2 cut(s) 283, 1144
BshFI GGCC 8 cut(s) 141, 228, 257, 282, 367, 426, 633, 1221
BshNI GGYRCC 1 cut(s) 1324
BsiEI CGRYCG 2 cut(s) 283, 1144
BsiHKAI GWGCWC 2 cut(s) 180, 503
BsiHKCI CYCGRG 1 cut(s) 251
BsiSI CCGG 1 cut(s) 1144
BslFI GGGAC 1 cut(s) 1139
BslI CCNNNNNNNGG 7 cut(s) 74, 251, 252, 328, 466, 1074, 1550
BsmAI GTCTC 1 cut(s) 467
BsmFI GGGAC 1 cut(s) 1139
BsmI GAATGC 1 cut(s) 1382
BsnI GGCC 8 cut(s) 141, 228, 257, 282, 367, 426, 633, 1221
BsoBI CYCGRG 1 cut(s) 251
Bsp1286I GDGCHC 3 cut(s) 180, 503, 695
Bsp143I GATC 5 cut(s) 222, 1050, 1055, 1116, 1417
Bsp1720I GCTNAGC 1 cut(s) 596
BspANI GGCC 8 cut(s) 141, 228, 257, 282, 367, 426, 633, 1221
BspCNI CTCAG 1 cut(s) 588
BspFNI CGCG 3 cut(s) 744, 1187, 1569
BspLI GGNNCC 7 cut(s) 256, 360, 692, 1042, 1066, 1079, 1326
BspMAI CTGCAG 1 cut(s) 1456
BspMI ACCTGC 1 cut(s) 317
BspPI GGATC 2 cut(s) 230, 1050
BspT107I GGYRCC 1 cut(s) 1324
BsrDI GCAATG 3 cut(s) 873, 1326, 1440
BsrI ACTGG 4 cut(s) 460, 518, 1372, 1595
BssECI CCNNGG 8 cut(s) 250, 277, 322, 362, 911, 1185, 1509, 1544
BssMI GATC 5 cut(s) 222, 1050, 1055, 1116, 1417
BssSI CACGAG 1 cut(s) 72
BssT1I CCWWGG 2 cut(s) 911, 1509
Bst2BI CACGAG 1 cut(s) 72
Bst2UI CCWGG 1 cut(s) 323
Bst4CI ACNGT 6 cut(s) 706, 782, 1129, 1162, 1198, 1390
Bst6I CTCTTC 1 cut(s) 245
BstC8I GCNNGC 4 cut(s) 440, 631, 1189, 1599
BstDEI CTNAG 4 cut(s) 495, 596, 1490, 1607
BstDSI CCRYGG 2 cut(s) 1185, 1544
BstEII GGTNACC 1 cut(s) 149
BstF5I GGATG 1 cut(s) 1180
BstFNI CGCG 3 cut(s) 744, 1187, 1569
BstH2I RGCGCY 1 cut(s) 1614
BstHHI GCGC 4 cut(s) 712, 744, 1571, 1613
BstKTI GATC 5 cut(s) 225, 1053, 1058, 1119, 1420
BstMAI GTCTC 1 cut(s) 467
BstMBI GATC 5 cut(s) 222, 1050, 1055, 1116, 1417
BstMCI CGRYCG 2 cut(s) 283, 1144
BstMWI GCNNNNNNNGC 6 cut(s) 184, 200, 263, 439, 548, 1491
BstNI CCWGG 1 cut(s) 323
BstPI GGTNACC 1 cut(s) 149
BstSCI CCNGG 1 cut(s) 321
BstSFI CTRYAG 3 cut(s) 528, 601, 1452
BstUI CGCG 3 cut(s) 744, 1187, 1569
BstV1I GCAGC 7 cut(s) 181, 535, 638, 971, 1265, 1438, 1466
BstX2I RGATCY 1 cut(s) 1055
BstXI CCANNNNNNTGG 1 cut(s) 1211
BstYI RGATCY 1 cut(s) 1055
BstZI CGGCCG 1 cut(s) 280
BsuRI GGCC 8 cut(s) 141, 228, 257, 282, 367, 426, 633, 1221
BtgI CCRYGG 2 cut(s) 1185, 1544
BtsCI GGATG 1 cut(s) 1180
BtsI GCAGTG 2 cut(s) 621, 808
BtsIMutI CAGTG 4 cut(s) 621, 808, 1339, 1365
BveI ACCTGC 1 cut(s) 317
Cac8I GCNNGC 4 cut(s) 440, 631, 1189, 1599
CaiI CAGNNNCTG 1 cut(s) 1338
CfoI GCGC 4 cut(s) 712, 744, 1571, 1613
Cfr13I GGNCC 3 cut(s) 255, 1064, 1219
Cfr42I CCGCGG 1 cut(s) 1188
CseI GACGC 1 cut(s) 879
Csp6I GTAC 3 cut(s) 163, 837, 1325
CspCI CAANNNNNGTGG 2 cut(s) 416, 451
CviAII CATG 4 cut(s) 160, 884, 1243, 1303
CviQI GTAC 3 cut(s) 163, 837, 1325
DdeI CTNAG 4 cut(s) 495, 596, 1490, 1607
DpnI GATC 5 cut(s) 224, 1052, 1057, 1118, 1419
DpnII GATC 5 cut(s) 222, 1050, 1055, 1116, 1417
DriI GACNNNNNGTC 1 cut(s) 756
EaeI YGGCCR 2 cut(s) 280, 424
EagI CGGCCG 1 cut(s) 280
Eam1104I CTCTTC 1 cut(s) 245
Eam1105I GACNNNNNGTC 1 cut(s) 756
EarI CTCTTC 1 cut(s) 245
Ecl136II GAGCTC 2 cut(s) 178, 501
EclXI CGGCCG 1 cut(s) 280
Eco130I CCWWGG 2 cut(s) 911, 1509
Eco147I AGGCCT 1 cut(s) 633
Eco24I GRGCYC 3 cut(s) 180, 503, 695
Eco47I GGWCC 1 cut(s) 1064
Eco47III AGCGCT 1 cut(s) 1612
Eco52I CGGCCG 1 cut(s) 280
Eco53kI GAGCTC 2 cut(s) 178, 501
Eco88I CYCGRG 1 cut(s) 251
Eco91I GGTNACC 1 cut(s) 149
EcoICRI GAGCTC 2 cut(s) 178, 501
EcoO65I GGTNACC 1 cut(s) 149
EcoRII CCWGG 1 cut(s) 321
EcoT14I CCWWGG 2 cut(s) 911, 1509
EcoT22I ATGCAT 1 cut(s) 1384
EcoT38I GRGCYC 3 cut(s) 180, 503, 695
ErhI CCWWGG 2 cut(s) 911, 1509
FaeI CATG 4 cut(s) 163, 887, 1246, 1306
FalI AAGNNNNNCTT 2 cut(s) 936, 968
FaqI GGGAC 1 cut(s) 1139
FatI CATG 4 cut(s) 159, 883, 1242, 1302
FauI CCCGC 3 cut(s) 1180, 1192, 1679
FauNDI CATATG 1 cut(s) 974
FbaI TGATCA 2 cut(s) 1050, 1417
FblI GTMKAC 1 cut(s) 774
FokI GGATG 1 cut(s) 1167
FriOI GRGCYC 3 cut(s) 180, 503, 695
FspBI CTAG 2 cut(s) 1103, 1259
GlaI GCGC 4 cut(s) 711, 743, 1570, 1612
GsaI CCCAGC 1 cut(s) 592
HaeII RGCGCY 1 cut(s) 1614
HaeIII GGCC 8 cut(s) 141, 228, 257, 282, 367, 426, 633, 1221
HapII CCGG 1 cut(s) 1144
HgaI GACGC 1 cut(s) 879
HhaI GCGC 4 cut(s) 712, 744, 1571, 1613
Hin1II CATG 4 cut(s) 163, 887, 1246, 1306
Hin6I GCGC 4 cut(s) 710, 742, 1569, 1611
HinP1I GCGC 4 cut(s) 710, 742, 1569, 1611
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HindIII AAGCTT 3 cut(s) 950, 1441, 1483
HinfI GANTC 5 cut(s) 269, 654, 907, 1147, 1398
HpaII CCGG 1 cut(s) 1144
HphI GGTGA 6 cut(s) 160, 529, 794, 806, 1378, 1550
Hpy166II GTNNAC 5 cut(s) 214, 775, 811, 1173, 1657
Hpy188III TCNNGA 7 cut(s) 37, 289, 374, 575, 700, 857, 1156
Hpy8I GTNNAC 5 cut(s) 214, 775, 811, 1173, 1657
Hpy99I CGWCG 4 cut(s) 113, 266, 706, 762
HpyAV CCTTC 5 cut(s) 371, 413, 457, 654, 706
HpyCH4III ACNGT 6 cut(s) 706, 782, 1129, 1162, 1198, 1390
HpyCH4IV ACGT 1 cut(s) 210
HpyF10VI GCNNNNNNNGC 6 cut(s) 184, 200, 263, 439, 548, 1491
HpyF3I CTNAG 4 cut(s) 495, 596, 1490, 1607
HpySE526I ACGT 1 cut(s) 210
Hsp92II CATG 4 cut(s) 163, 887, 1246, 1306
HspAI GCGC 4 cut(s) 710, 742, 1569, 1611
KpnI GGTACC 1 cut(s) 1328
Ksp22I TGATCA 2 cut(s) 1050, 1417
KspI CCGCGG 1 cut(s) 1188
Kzo9I GATC 5 cut(s) 222, 1050, 1055, 1116, 1417
LmnI GCTCC 3 cut(s) 183, 690, 1448
Lsp1109I GCAGC 7 cut(s) 181, 535, 638, 971, 1265, 1438, 1466
LweI GCATC 7 cut(s) 30, 737, 877, 1097, 1369, 1420, 1688
MaeI CTAG 2 cut(s) 1103, 1259
MaeII ACGT 1 cut(s) 210
MaeIII GTNAC 6 cut(s) 27, 149, 535, 1120, 1344, 1553
MalI GATC 5 cut(s) 224, 1052, 1057, 1118, 1419
MboI GATC 5 cut(s) 222, 1050, 1055, 1116, 1417
MboII GAAGA 4 cut(s) 97, 232, 499, 953
MflI RGATCY 1 cut(s) 1055
MhlI GDGCHC 3 cut(s) 180, 503, 695
MluCI AATT 3 cut(s) 845, 966, 1209
MlyI GAGTC 1 cut(s) 1392
MmeI TCCRAC 4 cut(s) 75, 740, 1228, 1459
Mph1103I ATGCAT 1 cut(s) 1384
MslI CAYNNNNRTG 3 cut(s) 704, 1247, 1578
MspA1I CMGCKG 2 cut(s) 194, 1187
MspI CCGG 1 cut(s) 1144
MspR9I CCNGG 1 cut(s) 323
Mva1269I GAATGC 1 cut(s) 1382
MvaI CCWGG 1 cut(s) 323
MvnI CGCG 3 cut(s) 744, 1187, 1569
MwoI GCNNNNNNNGC 6 cut(s) 184, 200, 263, 439, 548, 1491
NdeI CATATG 1 cut(s) 974
NdeII GATC 5 cut(s) 222, 1050, 1055, 1116, 1417
NlaIII CATG 4 cut(s) 163, 887, 1246, 1306
NlaIV GGNNCC 7 cut(s) 256, 360, 692, 1042, 1066, 1079, 1326
NmeAIII GCCGAG 2 cut(s) 258, 343
NmuCI GTSAC 1 cut(s) 535
NsiI ATGCAT 1 cut(s) 1384
OliI CACNNNNGTG 1 cut(s) 704
PaqCI CACCTGC 1 cut(s) 317
PceI AGGCCT 1 cut(s) 633
PcsI WCGNNNNNNNCGW 3 cut(s) 258, 370, 1125
PctI GAATGC 1 cut(s) 1382
PfeI GAWTC 4 cut(s) 269, 654, 907, 1147
PleI GAGTC 1 cut(s) 1392
PpsI GAGTC 1 cut(s) 1392
Psp124BI GAGCTC 2 cut(s) 180, 503
Psp6I CCWGG 1 cut(s) 321
PspEI GGTNACC 1 cut(s) 149
PspFI CCCAGC 1 cut(s) 588
PspGI CCWGG 1 cut(s) 321
PspN4I GGNNCC 7 cut(s) 256, 360, 692, 1042, 1066, 1079, 1326
PspPI GGNCC 3 cut(s) 255, 1064, 1219
PsrI GAACNNNNNNTAC 2 cut(s) 1113, 1145
PstI CTGCAG 1 cut(s) 1456
PstNI CAGNNNCTG 1 cut(s) 1338
PsuI RGATCY 1 cut(s) 1055
RsaI GTAC 3 cut(s) 164, 838, 1326
RsaNI GTAC 3 cut(s) 163, 837, 1325
RseI CAYNNNNRTG 3 cut(s) 704, 1247, 1578
SacI GAGCTC 2 cut(s) 180, 503
SacII CCGCGG 1 cut(s) 1188
Sau3AI GATC 5 cut(s) 222, 1050, 1055, 1116, 1417
Sau96I GGNCC 3 cut(s) 255, 1064, 1219
SchI GAGTC 1 cut(s) 1392
ScrFI CCNGG 1 cut(s) 323
SduI GDGCHC 3 cut(s) 180, 503, 695
SfaNI GCATC 7 cut(s) 30, 737, 877, 1097, 1369, 1420, 1688
SfcI CTRYAG 3 cut(s) 528, 601, 1452
Sfr303I CCGCGG 1 cut(s) 1188
SgrBI CCGCGG 1 cut(s) 1188
SinI GGWCC 1 cut(s) 1064
SmiMI CAYNNNNRTG 3 cut(s) 704, 1247, 1578
Sse9I AATT 3 cut(s) 845, 966, 1209
SseBI AGGCCT 1 cut(s) 633
SspMI CTAG 2 cut(s) 1103, 1259
SstI GAGCTC 2 cut(s) 180, 503
StuI AGGCCT 1 cut(s) 633
StyD4I CCNGG 1 cut(s) 321
StyI CCWWGG 2 cut(s) 911, 1509
TaaI ACNGT 6 cut(s) 706, 782, 1129, 1162, 1198, 1390
TaiI ACGT 1 cut(s) 213
TaqI TCGA 1 cut(s) 111
TasI AATT 3 cut(s) 845, 966, 1209
TauI GCSGC 7 cut(s) 92, 200, 203, 285, 426, 455, 728
TfiI GAWTC 4 cut(s) 269, 654, 907, 1147
TscAI CASTG 4 cut(s) 621, 808, 1339, 1372
TseFI GTSAC 1 cut(s) 535
TseI GCWGC 7 cut(s) 194, 548, 626, 984, 1253, 1451, 1454
Tsp45I GTSAC 1 cut(s) 535
TspDTI ATGAA 3 cut(s) 88, 1018, 1489
TspGWI ACGGA 1 cut(s) 950
TspRI CASTG 4 cut(s) 621, 808, 1339, 1372
VpaK11BI GGWCC 1 cut(s) 1064
XcmI CCANNNNNNNNNTGG 2 cut(s) 1240, 1335
XmiI GTMKAC 1 cut(s) 774
XspI CTAG 2 cut(s) 1103, 1259
Zsp2I ATGCAT 1 cut(s) 1384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.