2-alkenal reductase (NADP( )-dependent)-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 633 |
| AarI | CACCTGC | 1 cut(s) | 25 |
| Acc16I | TGCGCA | 1 cut(s) | 92 |
| Acc36I | ACCTGC | 2 cut(s) | 25, 908 |
| AccIII | TCCGGA | 1 cut(s) | 762 |
| AciI | CCGC | 2 cut(s) | 184, 687 |
| AclWI | GGATC | 1 cut(s) | 161 |
| AcoI | YGGCCR | 2 cut(s) | 96, 526 |
| AcsI | RAATTY | 1 cut(s) | 411 |
| AcuI | CTGAAG | 5 cut(s) | 36, 131, 617, 699, 741 |
| AfaI | GTAC | 5 cut(s) | 335, 671, 704, 729, 857 |
| AfeI | AGCGCT | 1 cut(s) | 889 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 245, 473 |
| AgsI | TTSAA | 5 cut(s) | 628, 664, 818, 875, 1021 |
| AhlI | ACTAGT | 1 cut(s) | 520 |
| AjnI | CCWGG | 1 cut(s) | 424 |
| AluBI | AGCT | 4 cut(s) | 115, 457, 659, 1007 |
| AluI | AGCT | 4 cut(s) | 115, 457, 659, 1007 |
| Alw26I | GTCTC | 5 cut(s) | 42, 344, 363, 473, 747 |
| AlwI | GGATC | 1 cut(s) | 161 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 503 |
| Aor13HI | TCCGGA | 1 cut(s) | 762 |
| Aor51HI | AGCGCT | 1 cut(s) | 889 |
| AoxI | GGCC | 4 cut(s) | 96, 526, 877, 1038 |
| ApeKI | GCWGC | 3 cut(s) | 115, 497, 656 |
| ApoI | RAATTY | 1 cut(s) | 411 |
| AspLEI | GCGC | 2 cut(s) | 93, 890 |
| AspS9I | GGNCC | 2 cut(s) | 760, 823 |
| AsuC2I | CCSGG | 2 cut(s) | 133, 231 |
| AsuHPI | GGTGA | 4 cut(s) | 49, 49, 365, 456 |
| AvaII | GGWCC | 2 cut(s) | 760, 823 |
| BalI | TGGCCA | 1 cut(s) | 528 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 839, 871 |
| BbvI | GCAGC | 3 cut(s) | 102, 509, 668 |
| BccI | CCATC | 1 cut(s) | 234 |
| BceAI | ACGGC | 1 cut(s) | 111 |
| BciT130I | CCWGG | 1 cut(s) | 426 |
| BclI | TGATCA | 1 cut(s) | 21 |
| BcnI | CCSGG | 2 cut(s) | 133, 231 |
| BcoDI | GTCTC | 5 cut(s) | 42, 344, 363, 473, 747 |
| BcuI | ACTAGT | 1 cut(s) | 520 |
| BfaI | CTAG | 1 cut(s) | 521 |
| BfoI | RGCGCY | 1 cut(s) | 891 |
| BfuAI | ACCTGC | 2 cut(s) | 25, 908 |
| BisI | GCNGC | 4 cut(s) | 116, 498, 657, 688 |
| BlsI | GCNGC | 4 cut(s) | 117, 499, 658, 689 |
| Bme1390I | CCNGG | 3 cut(s) | 133, 231, 426 |
| Bme18I | GGWCC | 2 cut(s) | 760, 823 |
| BmgT120I | GGNCC | 2 cut(s) | 760, 823 |
| BmiI | GGNNCC | 2 cut(s) | 127, 235 |
| BmrFI | CCNGG | 3 cut(s) | 133, 231, 426 |
| BmsI | GCATC | 4 cut(s) | 271, 509, 643, 756 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 446, 478 |
| BpmI | CTGGAG | 1 cut(s) | 525 |
| BpuEI | CTTGAG | 2 cut(s) | 731, 902 |
| BpuMI | CCSGG | 2 cut(s) | 133, 231 |
| BsaAI | YACGTR | 1 cut(s) | 859 |
| BsaJI | CCNNGG | 2 cut(s) | 132, 894 |
| BsaWI | WCCGGW | 2 cut(s) | 245, 762 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 468, 498 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 245, 473 |
| Bse118I | RCCGGY | 1 cut(s) | 59 |
| Bse1I | ACTGG | 3 cut(s) | 325, 412, 529 |
| BseAI | TCCGGA | 1 cut(s) | 762 |
| BseBI | CCWGG | 1 cut(s) | 426 |
| BseDI | CCNNGG | 2 cut(s) | 132, 894 |
| BseGI | GGATG | 4 cut(s) | 226, 286, 658, 771 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 245, 473 |
| BseMII | CTCAG | 1 cut(s) | 35 |
| BseNI | ACTGG | 3 cut(s) | 325, 412, 529 |
| BseXI | GCAGC | 3 cut(s) | 102, 509, 668 |
| BseYI | CCCAGC | 1 cut(s) | 324 |
| BshFI | GGCC | 4 cut(s) | 98, 528, 879, 1040 |
| BsiSI | CCGG | 5 cut(s) | 60, 132, 231, 246, 763 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 245, 473 |
| BsmAI | GTCTC | 5 cut(s) | 42, 344, 363, 473, 747 |
| BsmBI | CGTCTC | 1 cut(s) | 473 |
| BsnI | GGCC | 4 cut(s) | 98, 528, 879, 1040 |
| Bsp13I | TCCGGA | 1 cut(s) | 762 |
| Bsp1407I | TGTACA | 1 cut(s) | 727 |
| Bsp143I | GATC | 4 cut(s) | 21, 166, 693, 747 |
| BspACI | CCGC | 2 cut(s) | 184, 687 |
| BspANI | GGCC | 4 cut(s) | 98, 528, 879, 1040 |
| BspCNI | CTCAG | 1 cut(s) | 36 |
| BspEI | TCCGGA | 1 cut(s) | 762 |
| BspLI | GGNNCC | 2 cut(s) | 127, 235 |
| BspMI | ACCTGC | 2 cut(s) | 25, 908 |
| BspPI | GGATC | 1 cut(s) | 161 |
| BsrFI | RCCGGY | 1 cut(s) | 59 |
| BsrGI | TGTACA | 1 cut(s) | 727 |
| BsrI | ACTGG | 3 cut(s) | 325, 412, 529 |
| BssAI | RCCGGY | 1 cut(s) | 59 |
| BssECI | CCNNGG | 2 cut(s) | 132, 894 |
| BssMI | GATC | 4 cut(s) | 21, 166, 693, 747 |
| Bst2UI | CCWGG | 1 cut(s) | 426 |
| Bst4CI | ACNGT | 5 cut(s) | 53, 338, 357, 484, 702 |
| Bst6I | CTCTTC | 1 cut(s) | 324 |
| BstAUI | TGTACA | 1 cut(s) | 727 |
| BstBAI | YACGTR | 1 cut(s) | 859 |
| BstDEI | CTNAG | 3 cut(s) | 44, 268, 468 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 471 |
| BstF5I | GGATG | 4 cut(s) | 226, 286, 658, 771 |
| BstH2I | RGCGCY | 1 cut(s) | 891 |
| BstHHI | GCGC | 2 cut(s) | 93, 890 |
| BstKTI | GATC | 4 cut(s) | 24, 169, 696, 750 |
| BstMAI | GTCTC | 5 cut(s) | 42, 344, 363, 473, 747 |
| BstMBI | GATC | 4 cut(s) | 21, 166, 693, 747 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 506, 885, 896 |
| BstNI | CCWGG | 1 cut(s) | 426 |
| BstSCI | CCNGG | 3 cut(s) | 131, 229, 424 |
| BstV1I | GCAGC | 3 cut(s) | 102, 509, 668 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 544 |
| BsuRI | GGCC | 4 cut(s) | 98, 528, 879, 1040 |
| BtsCI | GGATG | 4 cut(s) | 226, 286, 658, 771 |
| BtsI | GCAGTG | 1 cut(s) | 345 |
| BtsIMutI | CAGTG | 2 cut(s) | 107, 345 |
| BveI | ACCTGC | 2 cut(s) | 25, 908 |
| CaiI | CAGNNNCTG | 1 cut(s) | 503 |
| CfoI | GCGC | 2 cut(s) | 93, 890 |
| Cfr10I | RCCGGY | 1 cut(s) | 59 |
| Cfr13I | GGNCC | 2 cut(s) | 760, 823 |
| Csp6I | GTAC | 5 cut(s) | 334, 670, 703, 728, 856 |
| CviAII | CATG | 5 cut(s) | 82, 175, 187, 544, 691 |
| CviQI | GTAC | 5 cut(s) | 334, 670, 703, 728, 856 |
| DdeI | CTNAG | 3 cut(s) | 44, 268, 468 |
| DpnI | GATC | 4 cut(s) | 23, 168, 695, 749 |
| DpnII | GATC | 4 cut(s) | 21, 166, 693, 747 |
| EaeI | YGGCCR | 2 cut(s) | 96, 526 |
| Eam1104I | CTCTTC | 1 cut(s) | 324 |
| EarI | CTCTTC | 1 cut(s) | 324 |
| Eco147I | AGGCCT | 1 cut(s) | 879 |
| Eco47I | GGWCC | 2 cut(s) | 760, 823 |
| Eco47III | AGCGCT | 1 cut(s) | 889 |
| Eco57I | CTGAAG | 5 cut(s) | 36, 131, 617, 699, 741 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 471 |
| EcoRI | GAATTC | 1 cut(s) | 411 |
| EcoRII | CCWGG | 1 cut(s) | 424 |
| Esp3I | CGTCTC | 1 cut(s) | 473 |
| FaeI | CATG | 5 cut(s) | 85, 178, 190, 547, 694 |
| FatI | CATG | 5 cut(s) | 81, 174, 186, 543, 690 |
| FbaI | TGATCA | 1 cut(s) | 21 |
| Fnu4HI | GCNGC | 4 cut(s) | 116, 498, 657, 688 |
| FokI | GGATG | 4 cut(s) | 213, 293, 665, 778 |
| Fsp4HI | GCNGC | 4 cut(s) | 116, 498, 657, 688 |
| FspBI | CTAG | 1 cut(s) | 521 |
| FspI | TGCGCA | 1 cut(s) | 92 |
| GlaI | GCGC | 2 cut(s) | 92, 889 |
| GluI | GCNGC | 4 cut(s) | 116, 498, 657, 688 |
| GsaI | CCCAGC | 1 cut(s) | 328 |
| GsuI | CTGGAG | 1 cut(s) | 525 |
| HaeII | RGCGCY | 1 cut(s) | 891 |
| HaeIII | GGCC | 4 cut(s) | 98, 528, 879, 1040 |
| HapII | CCGG | 5 cut(s) | 60, 132, 231, 246, 763 |
| HhaI | GCGC | 2 cut(s) | 93, 890 |
| Hin1II | CATG | 5 cut(s) | 85, 178, 190, 547, 694 |
| Hin6I | GCGC | 2 cut(s) | 91, 888 |
| HinP1I | GCGC | 2 cut(s) | 91, 888 |
| HincII | GTYRAC | 1 cut(s) | 518 |
| HindII | GTYRAC | 1 cut(s) | 518 |
| HinfI | GANTC | 8 cut(s) | 7, 74, 148, 272, 370, 641, 909, 1063 |
| HpaII | CCGG | 5 cut(s) | 60, 132, 231, 246, 763 |
| HphI | GGTGA | 4 cut(s) | 49, 49, 365, 456 |
| Hpy166II | GTNNAC | 2 cut(s) | 518, 728 |
| Hpy188I | TCNGA | 3 cut(s) | 16, 79, 747 |
| Hpy188III | TCNNGA | 5 cut(s) | 145, 504, 677, 763, 815 |
| Hpy8I | GTNNAC | 2 cut(s) | 518, 728 |
| HpyAV | CCTTC | 4 cut(s) | 716, 836, 841, 869 |
| HpyCH4III | ACNGT | 5 cut(s) | 53, 338, 357, 484, 702 |
| HpyCH4IV | ACGT | 2 cut(s) | 196, 858 |
| HpyCH4V | TGCA | 7 cut(s) | 284, 350, 656, 769, 917, 1046, 1078 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 506, 885, 896 |
| HpyF3I | CTNAG | 3 cut(s) | 44, 268, 468 |
| HpySE526I | ACGT | 2 cut(s) | 196, 858 |
| Hsp92II | CATG | 5 cut(s) | 85, 178, 190, 547, 694 |
| HspAI | GCGC | 2 cut(s) | 91, 888 |
| Kpn2I | TCCGGA | 1 cut(s) | 762 |
| Ksp22I | TGATCA | 1 cut(s) | 21 |
| Kzo9I | GATC | 4 cut(s) | 21, 166, 693, 747 |
| LmnI | GCTCC | 2 cut(s) | 506, 895 |
| Lsp1109I | GCAGC | 3 cut(s) | 102, 509, 668 |
| LweI | GCATC | 4 cut(s) | 271, 509, 643, 756 |
| MaeI | CTAG | 1 cut(s) | 521 |
| MaeII | ACGT | 2 cut(s) | 196, 858 |
| MaeIII | GTNAC | 2 cut(s) | 55, 982 |
| MalI | GATC | 4 cut(s) | 23, 168, 695, 749 |
| MboI | GATC | 4 cut(s) | 21, 166, 693, 747 |
| MboII | GAAGA | 4 cut(s) | 341, 610, 650, 875 |
| MlsI | TGGCCA | 1 cut(s) | 528 |
| MluCI | AATT | 6 cut(s) | 86, 411, 429, 628, 787, 933 |
| MluNI | TGGCCA | 1 cut(s) | 528 |
| MlyI | GAGTC | 3 cut(s) | 281, 903, 1057 |
| MmeI | TCCRAC | 3 cut(s) | 102, 541, 886 |
| MnlI | CCTC | 7 cut(s) | 22, 217, 402, 613, 751, 904, 973 |
| Mox20I | TGGCCA | 1 cut(s) | 528 |
| MroI | TCCGGA | 1 cut(s) | 762 |
| MscI | TGGCCA | 1 cut(s) | 528 |
| MseI | TTAA | 4 cut(s) | 366, 737, 786, 1084 |
| MslI | CAYNNNNRTG | 1 cut(s) | 801 |
| Msp20I | TGGCCA | 1 cut(s) | 528 |
| MspI | CCGG | 5 cut(s) | 60, 132, 231, 246, 763 |
| MspR9I | CCNGG | 3 cut(s) | 133, 231, 426 |
| MvaI | CCWGG | 1 cut(s) | 426 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 506, 885, 896 |
| NciI | CCSGG | 2 cut(s) | 133, 231 |
| NdeII | GATC | 4 cut(s) | 21, 166, 693, 747 |
| NlaIII | CATG | 5 cut(s) | 85, 178, 190, 547, 694 |
| NlaIV | GGNNCC | 2 cut(s) | 127, 235 |
| NmeAIII | GCCGAG | 1 cut(s) | 875 |
| NmuCI | GTSAC | 1 cut(s) | 55 |
| NsbI | TGCGCA | 1 cut(s) | 92 |
| PaqCI | CACCTGC | 1 cut(s) | 25 |
| PceI | AGGCCT | 1 cut(s) | 879 |
| PfeI | GAWTC | 4 cut(s) | 74, 148, 370, 641 |
| PflFI | GACNNNGTC | 1 cut(s) | 53 |
| PkrI | GCNGC | 4 cut(s) | 117, 499, 658, 689 |
| PleI | GAGTC | 3 cut(s) | 280, 903, 1057 |
| PpsI | GAGTC | 3 cut(s) | 280, 903, 1057 |
| Ppu21I | YACGTR | 1 cut(s) | 859 |
| PsiI | TTATAA | 1 cut(s) | 633 |
| Psp6I | CCWGG | 1 cut(s) | 424 |
| PspFI | CCCAGC | 1 cut(s) | 324 |
| PspGI | CCWGG | 1 cut(s) | 424 |
| PspN4I | GGNNCC | 2 cut(s) | 127, 235 |
| PspPI | GGNCC | 2 cut(s) | 760, 823 |
| PstNI | CAGNNNCTG | 1 cut(s) | 503 |
| PsyI | GACNNNGTC | 1 cut(s) | 53 |
| RsaI | GTAC | 5 cut(s) | 335, 671, 704, 729, 857 |
| RsaNI | GTAC | 5 cut(s) | 334, 670, 703, 728, 856 |
| RseI | CAYNNNNRTG | 1 cut(s) | 801 |
| SaqAI | TTAA | 4 cut(s) | 366, 737, 786, 1084 |
| SatI | GCNGC | 4 cut(s) | 116, 498, 657, 688 |
| Sau3AI | GATC | 4 cut(s) | 21, 166, 693, 747 |
| Sau96I | GGNCC | 2 cut(s) | 760, 823 |
| SchI | GAGTC | 3 cut(s) | 281, 903, 1057 |
| ScrFI | CCNGG | 3 cut(s) | 133, 231, 426 |
| SfaNI | GCATC | 4 cut(s) | 271, 509, 643, 756 |
| SinI | GGWCC | 2 cut(s) | 760, 823 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 801 |
| SmlI | CTYRAG | 2 cut(s) | 710, 881 |
| SmoI | CTYRAG | 2 cut(s) | 710, 881 |
| SpeI | ACTAGT | 1 cut(s) | 520 |
| Sse9I | AATT | 6 cut(s) | 86, 411, 429, 628, 787, 933 |
| SseBI | AGGCCT | 1 cut(s) | 879 |
| SsiI | CCGC | 2 cut(s) | 184, 687 |
| SspI | AATATT | 1 cut(s) | 388 |
| SspMI | CTAG | 1 cut(s) | 521 |
| StuI | AGGCCT | 1 cut(s) | 879 |
| StyD4I | CCNGG | 3 cut(s) | 131, 229, 424 |
| TaaI | ACNGT | 5 cut(s) | 53, 338, 357, 484, 702 |
| TaiI | ACGT | 2 cut(s) | 199, 861 |
| TaqI | TCGA | 1 cut(s) | 216 |
| TasI | AATT | 6 cut(s) | 86, 411, 429, 628, 787, 933 |
| TatI | WGTACW | 3 cut(s) | 333, 702, 727 |
| TauI | GCSGC | 1 cut(s) | 690 |
| TfiI | GAWTC | 4 cut(s) | 74, 148, 370, 641 |
| Tru1I | TTAA | 4 cut(s) | 366, 737, 786, 1084 |
| Tru9I | TTAA | 4 cut(s) | 366, 737, 786, 1084 |
| TscAI | CASTG | 2 cut(s) | 114, 352 |
| TseFI | GTSAC | 1 cut(s) | 55 |
| TseI | GCWGC | 3 cut(s) | 115, 497, 656 |
| Tsp45I | GTSAC | 1 cut(s) | 55 |
| TspDTI | ATGAA | 1 cut(s) | 919 |
| TspRI | CASTG | 2 cut(s) | 114, 352 |
| Tth111I | GACNNNGTC | 1 cut(s) | 53 |
| VpaK11BI | GGWCC | 2 cut(s) | 760, 823 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 471 |
| XapI | RAATTY | 1 cut(s) | 411 |
| XspI | CTAG | 1 cut(s) | 521 |