2-alkenal reductase (NADP( )-dependent)-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 460 |
| Acc36I | ACCTGC | 3 cut(s) | 22, 280, 460 |
| AccBSI | CCGCTC | 1 cut(s) | 247 |
| AciI | CCGC | 3 cut(s) | 247, 428, 454 |
| AcoI | YGGCCR | 1 cut(s) | 451 |
| AcsI | RAATTY | 1 cut(s) | 53 |
| AcuI | CTGAAG | 3 cut(s) | 65, 273, 301 |
| AfaI | GTAC | 3 cut(s) | 119, 236, 335 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 460 |
| AgsI | TTSAA | 4 cut(s) | 76, 149, 350, 407 |
| AjnI | CCWGG | 1 cut(s) | 309 |
| AluBI | AGCT | 2 cut(s) | 107, 212 |
| AluI | AGCT | 2 cut(s) | 107, 212 |
| Alw26I | GTCTC | 1 cut(s) | 279 |
| Ama87I | CYCGRG | 1 cut(s) | 242 |
| AoxI | GGCC | 3 cut(s) | 248, 409, 451 |
| ApeKI | GCWGC | 1 cut(s) | 104 |
| ApoI | RAATTY | 1 cut(s) | 53 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 128, 160 |
| AspS9I | GGNCC | 1 cut(s) | 292 |
| AvaI | CYCGRG | 1 cut(s) | 242 |
| AvaII | GGWCC | 1 cut(s) | 292 |
| BanII | GRGCYC | 1 cut(s) | 444 |
| BbsI | GAAGAC | 1 cut(s) | 307 |
| BbvI | GCAGC | 1 cut(s) | 116 |
| BccI | CCATC | 1 cut(s) | 122 |
| BciT130I | CCWGG | 1 cut(s) | 311 |
| BcoDI | GTCTC | 1 cut(s) | 279 |
| BfaI | CTAG | 2 cut(s) | 6, 98 |
| BfuAI | ACCTGC | 3 cut(s) | 22, 280, 460 |
| BisI | GCNGC | 4 cut(s) | 105, 248, 429, 454 |
| BlsI | GCNGC | 4 cut(s) | 106, 249, 430, 455 |
| Bme1390I | CCNGG | 1 cut(s) | 311 |
| Bme18I | GGWCC | 1 cut(s) | 292 |
| BmeT110I | CYCGRG | 1 cut(s) | 242 |
| BmgT120I | GGNCC | 1 cut(s) | 292 |
| BmrFI | CCNGG | 1 cut(s) | 311 |
| BmsI | GCATC | 3 cut(s) | 91, 156, 163 |
| BpiI | GAAGAC | 1 cut(s) | 307 |
| BpmI | CTGGAG | 1 cut(s) | 408 |
| BpuEI | CTTGAG | 1 cut(s) | 434 |
| BsaXI | ACNNNNNCTCC | 1 cut(s) | 33 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 460 |
| Bse3DI | GCAATG | 1 cut(s) | 463 |
| BseBI | CCWGG | 1 cut(s) | 311 |
| BseGI | GGATG | 1 cut(s) | 228 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 460 |
| BseMI | GCAATG | 1 cut(s) | 463 |
| BseXI | GCAGC | 1 cut(s) | 116 |
| BshFI | GGCC | 3 cut(s) | 250, 411, 453 |
| BsiHKCI | CYCGRG | 1 cut(s) | 242 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 460 |
| BsmAI | GTCTC | 1 cut(s) | 279 |
| BsmI | GAATGC | 1 cut(s) | 424 |
| BsnI | GGCC | 3 cut(s) | 250, 411, 453 |
| BsoBI | CYCGRG | 1 cut(s) | 242 |
| Bsp1286I | GDGCHC | 1 cut(s) | 444 |
| Bsp143I | GATC | 2 cut(s) | 37, 225 |
| BspACI | CCGC | 3 cut(s) | 247, 428, 454 |
| BspANI | GGCC | 3 cut(s) | 250, 411, 453 |
| BspMI | ACCTGC | 3 cut(s) | 22, 280, 460 |
| BsrBI | CCGCTC | 1 cut(s) | 247 |
| BsrDI | GCAATG | 1 cut(s) | 463 |
| BssMI | GATC | 2 cut(s) | 37, 225 |
| Bst2UI | CCWGG | 1 cut(s) | 311 |
| Bst4CI | ACNGT | 2 cut(s) | 234, 355 |
| BstF5I | GGATG | 1 cut(s) | 228 |
| BstKTI | GATC | 2 cut(s) | 40, 228 |
| BstMAI | GTCTC | 1 cut(s) | 279 |
| BstMBI | GATC | 2 cut(s) | 37, 225 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 202, 428 |
| BstNI | CCWGG | 1 cut(s) | 311 |
| BstSCI | CCNGG | 1 cut(s) | 309 |
| BstV1I | GCAGC | 1 cut(s) | 116 |
| BstV2I | GAAGAC | 1 cut(s) | 307 |
| BsuRI | GGCC | 3 cut(s) | 250, 411, 453 |
| BtsCI | GGATG | 1 cut(s) | 228 |
| BtsI | GCAGTG | 1 cut(s) | 183 |
| BtsIMutI | CAGTG | 2 cut(s) | 183, 360 |
| BveI | ACCTGC | 3 cut(s) | 22, 280, 460 |
| Cfr13I | GGNCC | 1 cut(s) | 292 |
| Csp6I | GTAC | 3 cut(s) | 118, 235, 334 |
| CviAII | CATG | 1 cut(s) | 190 |
| CviQI | GTAC | 3 cut(s) | 118, 235, 334 |
| DpnI | GATC | 2 cut(s) | 39, 227 |
| DpnII | GATC | 2 cut(s) | 37, 225 |
| EaeI | YGGCCR | 1 cut(s) | 451 |
| Eco147I | AGGCCT | 1 cut(s) | 411 |
| Eco24I | GRGCYC | 1 cut(s) | 444 |
| Eco47I | GGWCC | 1 cut(s) | 292 |
| Eco57I | CTGAAG | 3 cut(s) | 65, 273, 301 |
| Eco88I | CYCGRG | 1 cut(s) | 242 |
| EcoRII | CCWGG | 1 cut(s) | 309 |
| EcoT38I | GRGCYC | 1 cut(s) | 444 |
| FaeI | CATG | 1 cut(s) | 193 |
| FaiI | YATR | 7 cut(s) | 81, 191, 260, 264, 368, 388, 390 |
| FatI | CATG | 1 cut(s) | 189 |
| FauNDI | CATATG | 1 cut(s) | 388 |
| Fnu4HI | GCNGC | 4 cut(s) | 105, 248, 429, 454 |
| FokI | GGATG | 1 cut(s) | 235 |
| FriOI | GRGCYC | 1 cut(s) | 444 |
| Fsp4HI | GCNGC | 4 cut(s) | 105, 248, 429, 454 |
| FspBI | CTAG | 2 cut(s) | 6, 98 |
| GluI | GCNGC | 4 cut(s) | 105, 248, 429, 454 |
| GsuI | CTGGAG | 1 cut(s) | 408 |
| HaeIII | GGCC | 3 cut(s) | 250, 411, 453 |
| Hin1II | CATG | 1 cut(s) | 193 |
| Hpy188I | TCNGA | 2 cut(s) | 279, 296 |
| Hpy188III | TCNNGA | 3 cut(s) | 125, 347, 486 |
| HpyAV | CCTTC | 5 cut(s) | 108, 248, 296, 368, 401 |
| HpyCH4III | ACNGT | 2 cut(s) | 234, 355 |
| HpyCH4V | TGCA | 2 cut(s) | 104, 176 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 202, 428 |
| Hsp92II | CATG | 1 cut(s) | 193 |
| Kzo9I | GATC | 2 cut(s) | 37, 225 |
| LmnI | GCTCC | 1 cut(s) | 427 |
| Lsp1109I | GCAGC | 1 cut(s) | 116 |
| LweI | GCATC | 3 cut(s) | 91, 156, 163 |
| MaeI | CTAG | 2 cut(s) | 6, 98 |
| MaeIII | GTNAC | 1 cut(s) | 320 |
| MalI | GATC | 2 cut(s) | 39, 227 |
| MbiI | CCGCTC | 1 cut(s) | 247 |
| MboI | GATC | 2 cut(s) | 37, 225 |
| MboII | GAAGA | 4 cut(s) | 58, 307, 391, 407 |
| MhlI | GDGCHC | 1 cut(s) | 444 |
| MluCI | AATT | 3 cut(s) | 53, 265, 435 |
| MnlI | CCTC | 4 cut(s) | 15, 61, 186, 251 |
| MseI | TTAA | 2 cut(s) | 110, 285 |
| MspA1I | CMGCKG | 1 cut(s) | 428 |
| MspR9I | CCNGG | 1 cut(s) | 311 |
| Mva1269I | GAATGC | 1 cut(s) | 424 |
| MvaI | CCWGG | 1 cut(s) | 311 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 202, 428 |
| NdeI | CATATG | 1 cut(s) | 388 |
| NdeII | GATC | 2 cut(s) | 37, 225 |
| NlaIII | CATG | 1 cut(s) | 193 |
| PaeR7I | CTCGAG | 1 cut(s) | 242 |
| PaqCI | CACCTGC | 1 cut(s) | 460 |
| PceI | AGGCCT | 1 cut(s) | 411 |
| PctI | GAATGC | 1 cut(s) | 424 |
| PkrI | GCNGC | 4 cut(s) | 106, 249, 430, 455 |
| Psp6I | CCWGG | 1 cut(s) | 309 |
| PspGI | CCWGG | 1 cut(s) | 309 |
| PspPI | GGNCC | 1 cut(s) | 292 |
| PspXI | VCTCGAGB | 1 cut(s) | 242 |
| RsaI | GTAC | 3 cut(s) | 119, 236, 335 |
| RsaNI | GTAC | 3 cut(s) | 118, 235, 334 |
| SaqAI | TTAA | 2 cut(s) | 110, 285 |
| SatI | GCNGC | 4 cut(s) | 105, 248, 429, 454 |
| Sau3AI | GATC | 2 cut(s) | 37, 225 |
| Sau96I | GGNCC | 1 cut(s) | 292 |
| ScrFI | CCNGG | 1 cut(s) | 311 |
| SduI | GDGCHC | 1 cut(s) | 444 |
| SfaNI | GCATC | 3 cut(s) | 91, 156, 163 |
| Sfr274I | CTCGAG | 1 cut(s) | 242 |
| SinI | GGWCC | 1 cut(s) | 292 |
| SlaI | CTCGAG | 1 cut(s) | 242 |
| SmlI | CTYRAG | 2 cut(s) | 242, 413 |
| SmoI | CTYRAG | 2 cut(s) | 242, 413 |
| Sse9I | AATT | 3 cut(s) | 53, 265, 435 |
| SseBI | AGGCCT | 1 cut(s) | 411 |
| SsiI | CCGC | 3 cut(s) | 247, 428, 454 |
| SspMI | CTAG | 2 cut(s) | 6, 98 |
| StuI | AGGCCT | 1 cut(s) | 411 |
| StyD4I | CCNGG | 1 cut(s) | 309 |
| TaaI | ACNGT | 2 cut(s) | 234, 355 |
| TaqI | TCGA | 1 cut(s) | 243 |
| TasI | AATT | 3 cut(s) | 53, 265, 435 |
| TatI | WGTACW | 1 cut(s) | 234 |
| TauI | GCSGC | 3 cut(s) | 250, 431, 456 |
| Tru1I | TTAA | 2 cut(s) | 110, 285 |
| Tru9I | TTAA | 2 cut(s) | 110, 285 |
| TscAI | CASTG | 2 cut(s) | 183, 360 |
| TseI | GCWGC | 1 cut(s) | 104 |
| TspGWI | ACGGA | 1 cut(s) | 351 |
| TspRI | CASTG | 2 cut(s) | 183, 360 |
| VpaK11BI | GGWCC | 1 cut(s) | 292 |
| XapI | RAATTY | 1 cut(s) | 53 |
| XhoI | CTCGAG | 1 cut(s) | 242 |
| XspI | CTAG | 2 cut(s) | 6, 98 |