MD02G1002500.v1.1

2-alkenal reductase (NADP( )-dependent)-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000196
Physical Location & Seq
Forward (+)
212151 .. 215183
3033 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1002500.v1.1.491

Sequence Viewer

Length: 1038 bp
ATGGAAGTGACGAGCAACAAGCAGCTGATACTCAGAGACTATGTCACTGGCTTCCCCAAAGAATCCGATATGCAATTAGTCACCGCCGCAGCCACCAAGCTGAAGCTTCCAGAAGGTTCCACTGGGGTTTTGGTGAAGAACCTCTACTTGTCCTGCGACCCTTATATGAGAGACCGCATGACCAAGCGCGAACCCGGCGGCAGTTATGTCCCCTCCTTCGTGCCCGGTTCGCCTATAACCGGATACGGAGTGGCTAAAGTTTTGGAATCGGGGGATCCCAATTTCAAGGAAGGCGACTTTGTTTGGGGGATGACCGGGTGGGAAGAATATAGTCTCATCACTGCCACACAGGCCCTGTTTAAGATTCACGATACCGATGTCCCACTCTCTTACTATACTGGAATTCTAGGTATGCCTGGTATAACTGCTTATGCTGGTTTTCATGAGGTTTGCACGCCAAAGAAAGGAGAGACAGTCTTCATTTCTGCCGCATCTGGAGCAGTAGGTCAGCTAGTTGGGCAATTTGCGAAGTTGTTGGGTTGCTATGTTGTTGGGAGTGCTGGAAGCAAAGAAAAGGTTGATCTGCTGAGGAACAAGTTCGGGTTTGATGAGGCTTTCAATTATAAAGAAGAACCTAACTTGGATGCTGCCTTAAAAAGGTACTTTCCTGAAGGCATCGATATATACTTTGAAAATGTTGGGGGAAAGATGCTCGATGCAGTCCTACTAAACATGAGGACGAATGGCCGAATCGCAGTTTGTGGGATGATCTCGCAGTACAACCTTGAGCAGAAAGAAGGCGTGCATAATTTAACGTCAATGATCTCTAAACAGCTCCGTATGCAAGGTTTCGTGGCTTCTAGTTTCTATCATCTCTACGGGAAGTTTCTTGAAACTGTACTACCATTCATAAAAGAAGGGAAGATAACATACGTGGAAGATGTAGTTGAAGGCATTGAGAGCGCTCCGGCAGCTCTGATAGGGCTCTTTGCCGGCCGCAATGTCGGAAAGCAGGTGGTTTTAGTTTCCAGGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006950 GO:0006979 GO:0008150 GO:0016020 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N_2 ADH_zinc_N

Protein Analysis

346

Amino Acids

37.82

Weight (kDa)

5.93

Isoelectric Point (pI)

31.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N_2 PF16884 7 - 117 8.6e-28 N-terminal domain of oxidoreductase
ADH_zinc_N PF00107 167 - 291 3.1e-23 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 201 - 340 1.1e-08 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000196)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65560
fragaria_vesca FvH4_1g00330 FvH4_1g00342 FvH4_1g00343 FvH4_1g00344 FvH4_1g00345 FvH4_1g00345 FvH4_1g00346 FvH4_1g26520
malus_domestica MD02G1001800.v1.1 MD02G1001900.v1.1 MD02G1002100.v1.1 MD02G1002300.v1.1 MD02G1002400.v1.1 MD02G1002500.v1.1 MD02G1002600.v1.1 MD15G1145700.v1.1
prunus_persica Prupe.7G268600_v2.0.a1 Prupe.7G268700_v2.0.a1 Prupe.7G268800_v2.0.a1 Prupe.7G268800_v2.0.a1 Prupe.7G268900_v2.0.a1
pyrus_communis pycom02g00050 pycom02g00060 pycom02g00100 pycom02g00120 pycom02g00130 pycom15g13090 pycom15g13100
rosa_chinensis RchiOBHm_Chr1g0325321 RchiOBHm_Chr1g0325341 RchiOBHm_Chr1g0325351 RchiOBHm_Chr1g0325361 RchiOBHm_Chr1g0364881 RchiOBHm_Chr1g0364891 RchiOBHm_Chr2g0084701 RchiOBHm_Chr2g0084721 RchiOBHm_Chr2g0084731 RchiOBHm_Chr2g0084741 RchiOBHm_Chr2g0084751 RchiOBHm_Chr2g0084761 RchiOBHm_Chr2g0084771 RchiOBHm_Chr2g0085311 RchiOBHm_Chr2g0085321 RchiOBHm_Chr2g0085341 RchiOBHm_Chr2g0085351 RchiOBHm_Chr2g0117721 RchiOBHm_Chr2g0159631 RchiOBHm_Chr3g0484011 RchiOBHm_Chr5g0021141 RchiOBHm_Chr5g0061081 RchiOBHm_Chr6g0275731 RchiOBHm_Chr7g0238011
rosa_laevigata RLG00000013438 RLG00000015632 RLG00000015634 RLG00000015635 RLG00000015636 RLG00000015637 RLG00000015677 RLG00000015679 RLG00000030145 RLG00000030146 RLG00000035427
rosa_multiflora Rmu_co8334849.1_g000001 Rmu_sc0001366.1_g000035 Rmu_sc0002352.1_g000003 Rmu_sc0002352.1_g000004 Rmu_sc0002352.1_g000006 Rmu_sc0002352.1_g000007 Rmu_sc0002352.1_g000009 Rmu_sc0002586.1_g000008 Rmu_sc0004966.1_g000016 Rmu_sc0007122.1_g000001 Rmu_sc0008804.1_g000002 Rmu_sc0008883.1_g000001 Rmu_sc0010860.1_g000002 Rmu_sc0012119.1_g000001 Rmu_sc0012119.1_g000003 Rmu_sc0012119.1_g000006 Rmu_sc0012119.1_g000007 Rmu_sc0012119.1_g000009 Rmu_sc0020031.1_g000004 Rmu_sc0020800.1_g000005 Rmu_sc0020800.1_g000007 Rmu_sc0020800.1_g000008 Rmu_sc0020800.1_g000009 Rmu_sc0025597.1_g000001 Rmu_sc0029132.1_g000002
rosa_roxburghii Rroxscaffold_2G00155450 Rroxscaffold_2G00155460 Rroxscaffold_2G00155470 Rroxscaffold_2G00155830 Rroxscaffold_2G00155840 Rroxscaffold_2G00155850 Rroxscaffold_2G00155860 Rroxscaffold_2G00155870 Rroxscaffold_2G00155910 Rroxscaffold_3G00273850 Rroxscaffold_4G00324820 Rroxscaffold_7G00192930 Rroxscaffold_7G00192960
rosa_rugosa Rorug01G0051300 Rorug01G0051400 Rorug01G0455700 Rorug01G0455800 Rorug01G0455900 Rorug01G0459500 Rorug01G0459500 Rorug01G0459600 Rorug01G0459700 Rorug01G0459700 Rorug02G0335000
rosa_samantha Rh1AG066500 Rh1BG054700 Rh1BG054800 Rh1BG176600 Rh2BG004400 Rh2BG004500 Rh2BG004600 Rh2BG004700 Rh2BG008600 Rh2BG008700 Rh2CG004500 Rh2CG004700 Rh2CG004800 Rh2CG004900 Rh2CG005000 Rh2CG005100 Rh2CG005200 Rh2CG009300 Rh2CG009400 Rh2DG004200 Rh2DG004300 Rh2DG004400 Rh2DG004500 Rh2DG004600 Rh2DG009800 Rh5BG411800 Rh5CG167200 Rh5CG436800 Rh5CG578800 Rh6DG203800
rosa_wichuraiana Rw1G005560 Rw2G000320 Rw2G000350 Rw2G000360 Rw2G000370 Rw2G000750 Rw2G000760 Rw4G022130 Rw5G015810 Rw5G037620 Rw6G018120 Rw7G038530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 624
AarI CACCTGC 1 cut(s) 1003
Acc36I ACCTGC 1 cut(s) 1003
AccII CGCG 1 cut(s) 189
AciI CCGC 6 cut(s) 84, 87, 175, 198, 489, 997
AclWI GGATC 2 cut(s) 269, 282
AcoI YGGCCR 2 cut(s) 745, 994
AcsI RAATTY 1 cut(s) 402
AcuI CTGAAG 2 cut(s) 122, 690
AfaI GTAC 3 cut(s) 662, 779, 900
AfeI AGCGCT 1 cut(s) 964
AfiI CCNNNNNNNGG 3 cut(s) 239, 464, 657
AgsI TTSAA 5 cut(s) 286, 619, 692, 893, 950
AjnI CCWGG 2 cut(s) 415, 1028
AluBI AGCT 6 cut(s) 25, 100, 106, 511, 835, 974
AluI AGCT 6 cut(s) 25, 100, 106, 511, 835, 974
Alw26I GTCTC 4 cut(s) 30, 165, 338, 464
AlwI GGATC 2 cut(s) 269, 282
AlwNI CAGNNNCTG 1 cut(s) 355
Aor51HI AGCGCT 1 cut(s) 964
AoxI GGCC 3 cut(s) 351, 745, 994
ApeKI GCWGC 4 cut(s) 22, 89, 647, 971
ApoI RAATTY 1 cut(s) 402
Asp700I GAANNNNTTC 1 cut(s) 596
AspLEI GCGC 2 cut(s) 189, 965
AspS9I GGNCC 1 cut(s) 352
AsuC2I CCSGG 3 cut(s) 195, 225, 316
AsuHPI GGTGA 2 cut(s) 73, 145
BaeGI GKGCMC 1 cut(s) 225
BaeI ACNNNNGTAYC 2 cut(s) 363, 396
BamHI GGATCC 1 cut(s) 274
BanII GRGCYC 1 cut(s) 987
BarI GAAGNNNNNNTAC 4 cut(s) 128, 160, 914, 946
BbsI GAAGAC 1 cut(s) 469
BbvCI CCTCAGC 1 cut(s) 587
BbvI GCAGC 4 cut(s) 34, 101, 634, 983
BciT130I CCWGG 2 cut(s) 417, 1030
BciVI GTATCC 1 cut(s) 236
BcnI CCSGG 3 cut(s) 195, 225, 316
BcoDI GTCTC 4 cut(s) 30, 165, 338, 464
BfaI CTAG 3 cut(s) 407, 512, 861
BfoI RGCGCY 1 cut(s) 966
BfuAI ACCTGC 1 cut(s) 1003
BfuI GTATCC 1 cut(s) 236
BglI GCCNNNNNGGC 1 cut(s) 350
BisI GCNGC 8 cut(s) 23, 87, 90, 199, 489, 648, 972, 997
BlsI GCNGC 8 cut(s) 24, 88, 91, 200, 490, 649, 973, 998
Bme1390I CCNGG 5 cut(s) 195, 225, 316, 417, 1030
BmgT120I GGNCC 1 cut(s) 352
BmiI GGNNCC 2 cut(s) 118, 276
BmrFI CCNGG 5 cut(s) 195, 225, 316, 417, 1030
BmrI ACTGGG 1 cut(s) 132
BmsI GCATC 5 cut(s) 500, 634, 684, 699, 706
BmuI ACTGGG 1 cut(s) 132
BpiI GAAGAC 1 cut(s) 469
BpmI CTGGAG 1 cut(s) 516
Bpu10I CCTNAGC 1 cut(s) 587
BpuEI CTTGAG 1 cut(s) 806
BpuMI CCSGG 3 cut(s) 195, 225, 316
Bsa29I ATCGAT 1 cut(s) 678
BsaAI YACGTR 1 cut(s) 934
BsaI GGTCTC 1 cut(s) 165
BsaJI CCNNGG 1 cut(s) 1029
BsaWI WCCGGW 1 cut(s) 239
BsaXI ACNNNNNCTCC 2 cut(s) 459, 489
Bsc4I CCNNNNNNNGG 3 cut(s) 239, 464, 657
Bse118I RCCGGY 1 cut(s) 992
Bse1I ACTGG 3 cut(s) 52, 127, 403
Bse3DI GCAATG 1 cut(s) 1006
BseBI CCWGG 2 cut(s) 417, 1030
BseCI ATCGAT 1 cut(s) 678
BseDI CCNNGG 1 cut(s) 1029
BseGI GGATG 3 cut(s) 315, 649, 771
BseLI CCNNNNNNNGG 3 cut(s) 239, 464, 657
BseMI GCAATG 1 cut(s) 1006
BseMII CTCAG 2 cut(s) 46, 578
BseNI ACTGG 3 cut(s) 52, 127, 403
BseSI GKGCMC 1 cut(s) 225
BseX3I CGGCCG 1 cut(s) 994
BseXI GCAGC 4 cut(s) 34, 101, 634, 983
Bsh1236I CGCG 1 cut(s) 189
Bsh1285I CGRYCG 1 cut(s) 997
BshFI GGCC 3 cut(s) 353, 747, 996
BshVI ATCGAT 1 cut(s) 678
BsiEI CGRYCG 1 cut(s) 997
BsiSI CCGG 6 cut(s) 195, 225, 240, 315, 968, 993
BslFI GGGAC 2 cut(s) 194, 365
BslI CCNNNNNNNGG 3 cut(s) 239, 464, 657
BsmAI GTCTC 4 cut(s) 30, 165, 338, 464
BsmFI GGGAC 2 cut(s) 194, 365
BsnI GGCC 3 cut(s) 353, 747, 996
Bso31I GGTCTC 1 cut(s) 165
Bsp1286I GDGCHC 2 cut(s) 225, 987
Bsp143I GATC 4 cut(s) 274, 580, 768, 822
BspACI CCGC 6 cut(s) 84, 87, 175, 198, 489, 997
BspANI GGCC 3 cut(s) 353, 747, 996
BspCNI CTCAG 2 cut(s) 45, 579
BspDI ATCGAT 1 cut(s) 678
BspFNI CGCG 1 cut(s) 189
BspHI TCATGA 1 cut(s) 442
BspLI GGNNCC 2 cut(s) 118, 276
BspMI ACCTGC 1 cut(s) 1003
BspPI GGATC 2 cut(s) 269, 282
BspTNI GGTCTC 1 cut(s) 165
BsrDI GCAATG 1 cut(s) 1006
BsrFI RCCGGY 1 cut(s) 992
BsrI ACTGG 3 cut(s) 52, 127, 403
BssAI RCCGGY 1 cut(s) 992
BssECI CCNNGG 1 cut(s) 1029
BssMI GATC 4 cut(s) 274, 580, 768, 822
Bst2UI CCWGG 2 cut(s) 417, 1030
Bst4CI ACNGT 2 cut(s) 475, 898
BstBAI YACGTR 1 cut(s) 934
BstC8I GCNNGC 3 cut(s) 455, 803, 994
BstDEI CTNAG 2 cut(s) 32, 587
BstENI CCTNNNNNAGG 1 cut(s) 655
BstF5I GGATG 3 cut(s) 315, 649, 771
BstFNI CGCG 1 cut(s) 189
BstH2I RGCGCY 1 cut(s) 966
BstHHI GCGC 2 cut(s) 189, 965
BstKTI GATC 4 cut(s) 277, 583, 771, 825
BstMAI GTCTC 4 cut(s) 30, 165, 338, 464
BstMBI GATC 4 cut(s) 274, 580, 768, 822
BstMCI CGRYCG 1 cut(s) 997
BstMWI GCNNNNNNNGC 8 cut(s) 195, 229, 350, 497, 517, 841, 960, 971
BstNI CCWGG 2 cut(s) 417, 1030
BstSCI CCNGG 5 cut(s) 193, 223, 314, 415, 1028
BstSLI GKGCMC 1 cut(s) 225
BstUI CGCG 1 cut(s) 189
BstV1I GCAGC 4 cut(s) 34, 101, 634, 983
BstV2I GAAGAC 1 cut(s) 469
BstX2I RGATCY 1 cut(s) 274
BstYI RGATCY 1 cut(s) 274
BstZI CGGCCG 1 cut(s) 994
Bsu15I ATCGAT 1 cut(s) 678
BsuI GTATCC 1 cut(s) 236
BsuRI GGCC 3 cut(s) 353, 747, 996
BsuTUI ATCGAT 1 cut(s) 678
BtsCI GGATG 3 cut(s) 315, 649, 771
BtsI GCAGTG 1 cut(s) 339
BtsIMutI CAGTG 3 cut(s) 45, 120, 339
BveI ACCTGC 1 cut(s) 1003
Cac8I GCNNGC 3 cut(s) 455, 803, 994
CaiI CAGNNNCTG 1 cut(s) 355
CciI TCATGA 1 cut(s) 442
CfoI GCGC 2 cut(s) 189, 965
Cfr10I RCCGGY 1 cut(s) 992
Cfr13I GGNCC 1 cut(s) 352
ClaI ATCGAT 1 cut(s) 678
Csp6I GTAC 3 cut(s) 661, 778, 899
CviAII CATG 3 cut(s) 178, 443, 733
CviQI GTAC 3 cut(s) 661, 778, 899
DdeI CTNAG 2 cut(s) 32, 587
DpnI GATC 4 cut(s) 276, 582, 770, 824
DpnII GATC 4 cut(s) 274, 580, 768, 822
EaeI YGGCCR 2 cut(s) 745, 994
EagI CGGCCG 1 cut(s) 994
EclXI CGGCCG 1 cut(s) 994
Eco24I GRGCYC 1 cut(s) 987
Eco31I GGTCTC 1 cut(s) 165
Eco47III AGCGCT 1 cut(s) 964
Eco52I CGGCCG 1 cut(s) 994
Eco57I CTGAAG 2 cut(s) 122, 690
EcoNI CCTNNNNNAGG 1 cut(s) 655
EcoO109I RGGNCCY 1 cut(s) 352
EcoRI GAATTC 1 cut(s) 402
EcoRII CCWGG 2 cut(s) 415, 1028
EcoT38I GRGCYC 1 cut(s) 987
FaeI CATG 3 cut(s) 181, 446, 736
FaqI GGGAC 2 cut(s) 194, 365
FatI CATG 3 cut(s) 177, 442, 732
Fnu4HI GCNGC 8 cut(s) 23, 87, 90, 199, 489, 648, 972, 997
FokI GGATG 3 cut(s) 322, 656, 778
FriOI GRGCYC 1 cut(s) 987
Fsp4HI GCNGC 8 cut(s) 23, 87, 90, 199, 489, 648, 972, 997
FspBI CTAG 3 cut(s) 407, 512, 861
GlaI GCGC 2 cut(s) 188, 964
GluI GCNGC 8 cut(s) 23, 87, 90, 199, 489, 648, 972, 997
GsuI CTGGAG 1 cut(s) 516
HaeII RGCGCY 1 cut(s) 966
HaeIII GGCC 3 cut(s) 353, 747, 996
HapII CCGG 6 cut(s) 195, 225, 240, 315, 968, 993
HhaI GCGC 2 cut(s) 189, 965
Hin1II CATG 3 cut(s) 181, 446, 736
Hin6I GCGC 2 cut(s) 187, 963
HinP1I GCGC 2 cut(s) 187, 963
HindIII AAGCTT 1 cut(s) 104
HinfI GANTC 4 cut(s) 62, 266, 364, 750
HpaII CCGG 6 cut(s) 195, 225, 240, 315, 968, 993
HphI GGTGA 2 cut(s) 73, 145
Hpy188I TCNGA 4 cut(s) 35, 67, 978, 1007
Hpy188III TCNNGA 6 cut(s) 110, 368, 443, 495, 668, 890
HpyAV CCTTC 7 cut(s) 107, 226, 284, 665, 791, 911, 944
HpyCH4III ACNGT 2 cut(s) 475, 898
HpyCH4IV ACGT 2 cut(s) 815, 933
HpyCH4V TGCA 5 cut(s) 73, 453, 719, 805, 844
HpyF10VI GCNNNNNNNGC 8 cut(s) 195, 229, 350, 497, 517, 841, 960, 971
HpyF3I CTNAG 2 cut(s) 32, 587
HpySE526I ACGT 2 cut(s) 815, 933
Hsp92II CATG 3 cut(s) 181, 446, 736
HspAI GCGC 2 cut(s) 187, 963
KroI GCCGGC 1 cut(s) 992
KroNI GCCGGC 1 cut(s) 994
Kzo9I GATC 4 cut(s) 274, 580, 768, 822
LmnI GCTCC 3 cut(s) 497, 840, 970
Lsp1109I GCAGC 4 cut(s) 34, 101, 634, 983
LweI GCATC 5 cut(s) 500, 634, 684, 699, 706
MaeI CTAG 3 cut(s) 407, 512, 861
MaeII ACGT 2 cut(s) 815, 933
MaeIII GTNAC 3 cut(s) 7, 43, 79
MalI GATC 4 cut(s) 276, 582, 770, 824
MboI GATC 4 cut(s) 274, 580, 768, 822
MboII GAAGA 6 cut(s) 148, 335, 469, 641, 934, 950
MflI RGATCY 1 cut(s) 274
MhlI GDGCHC 2 cut(s) 225, 987
MluCI AATT 6 cut(s) 74, 280, 402, 521, 619, 808
MmeI TCCRAC 1 cut(s) 985
MnlI CCTC 6 cut(s) 152, 223, 439, 582, 604, 729
MroNI GCCGGC 1 cut(s) 992
MroXI GAANNNNTTC 1 cut(s) 596
MseI TTAA 3 cut(s) 360, 653, 812
MspA1I CMGCKG 1 cut(s) 25
MspI CCGG 6 cut(s) 195, 225, 240, 315, 968, 993
MspR9I CCNGG 5 cut(s) 195, 225, 316, 417, 1030
MvaI CCWGG 2 cut(s) 417, 1030
MvnI CGCG 1 cut(s) 189
MwoI GCNNNNNNNGC 8 cut(s) 195, 229, 350, 497, 517, 841, 960, 971
NaeI GCCGGC 1 cut(s) 994
NciI CCSGG 3 cut(s) 195, 225, 316
NdeII GATC 4 cut(s) 274, 580, 768, 822
NgoMIV GCCGGC 1 cut(s) 992
NlaIII CATG 3 cut(s) 181, 446, 736
NlaIV GGNNCC 2 cut(s) 118, 276
NmuCI GTSAC 3 cut(s) 7, 43, 79
PagI TCATGA 1 cut(s) 442
PaqCI CACCTGC 1 cut(s) 1003
PdiI GCCGGC 1 cut(s) 994
PdmI GAANNNNTTC 1 cut(s) 596
PfeI GAWTC 4 cut(s) 62, 266, 364, 750
PflFI GACNNNGTC 1 cut(s) 41
PkrI GCNGC 8 cut(s) 24, 88, 91, 200, 490, 649, 973, 998
Ppu21I YACGTR 1 cut(s) 934
PsiI TTATAA 1 cut(s) 624
Psp6I CCWGG 2 cut(s) 415, 1028
PspGI CCWGG 2 cut(s) 415, 1028
PspN4I GGNNCC 2 cut(s) 118, 276
PspPI GGNCC 1 cut(s) 352
PstNI CAGNNNCTG 1 cut(s) 355
PsuI RGATCY 1 cut(s) 274
PsyI GACNNNGTC 1 cut(s) 41
PvuII CAGCTG 1 cut(s) 25
RsaI GTAC 3 cut(s) 662, 779, 900
RsaNI GTAC 3 cut(s) 661, 778, 899
SaqAI TTAA 3 cut(s) 360, 653, 812
SatI GCNGC 8 cut(s) 23, 87, 90, 199, 489, 648, 972, 997
Sau3AI GATC 4 cut(s) 274, 580, 768, 822
Sau96I GGNCC 1 cut(s) 352
ScrFI CCNGG 5 cut(s) 195, 225, 316, 417, 1030
SduI GDGCHC 2 cut(s) 225, 987
SfaNI GCATC 5 cut(s) 500, 634, 684, 699, 706
SmlI CTYRAG 1 cut(s) 785
SmoI CTYRAG 1 cut(s) 785
Sse9I AATT 6 cut(s) 74, 280, 402, 521, 619, 808
SsiI CCGC 6 cut(s) 84, 87, 175, 198, 489, 997
SspMI CTAG 3 cut(s) 407, 512, 861
StyD4I CCNGG 5 cut(s) 193, 223, 314, 415, 1028
TaaI ACNGT 2 cut(s) 475, 898
TaiI ACGT 2 cut(s) 818, 936
TaqI TCGA 2 cut(s) 678, 714
TasI AATT 6 cut(s) 74, 280, 402, 521, 619, 808
TatI WGTACW 2 cut(s) 777, 898
TauI GCSGC 4 cut(s) 89, 201, 491, 999
TfiI GAWTC 4 cut(s) 62, 266, 364, 750
Tru1I TTAA 3 cut(s) 360, 653, 812
Tru9I TTAA 3 cut(s) 360, 653, 812
TscAI CASTG 3 cut(s) 52, 127, 346
TseFI GTSAC 3 cut(s) 7, 43, 79
TseI GCWGC 4 cut(s) 22, 89, 647, 971
Tsp45I GTSAC 3 cut(s) 7, 43, 79
TspDTI ATGAA 3 cut(s) 431, 469, 898
TspGWI ACGGA 2 cut(s) 261, 827
TspRI CASTG 3 cut(s) 52, 127, 346
Tth111I GACNNNGTC 1 cut(s) 41
XagI CCTNNNNNAGG 1 cut(s) 655
XapI RAATTY 1 cut(s) 402
XcmI CCANNNNNNNNNTGG 1 cut(s) 127
XmnI GAANNNNTTC 1 cut(s) 596
XspI CTAG 3 cut(s) 407, 512, 861
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.