RchiOBHm_Chr5g0021141

2-alkenal reductase (NADP( )-dependent)-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000196
Physical Location & Seq
Forward (+)
15430089 .. 15432179
2091 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30121

Sequence Viewer

Length: 678 bp
ATGGCTCTATTTTTTCTTGCTGAAAATGGTGCTAGCTTAGGTATCTCATCTGGTATTGTGGAAGCTGTATATATCACCGGCTTCCCCAAAGAATCCGACCTCAAATTGACCACTGCCACTACCAAACTCAAGCTTCCAGAAGGTTCGACTGGCCTCCTCGTCAAGAACCTCTACTTGTCCTGTGATCCTTACATGCGAAGCTGTATGACCAAGCAGAAGGAAGAGGGTGAGGAGGGGGGCTACATATTGCTGTTATTTGTTTATAATATTGCTCAAGGATTGTTGCATTGTCCTGTAATTGGTTTGGGAGTGGCTAAAGTCCTGGAATCTAGGGATCCAAAGTTTAAGCCAGGGGACTTGGTTTGGGGTCACACTGGTTGGGAAGAATATAGTGTCATCACCACAGAGTCTCTGATTAAGATTCACACACTGATGTGCCTCTCTCTTACTATACTGGACTTCTCGTCTACTGGTTATTTGGGCTCATCTGGCCTATTCATATGTTATGTAGGTATGCCTGGAGTGACTGCTTATGCTGGTTTTTATGAGATCTGCTCTCCTAAGAAAGGAGAGACAGTCTACATTTCAGCCGCATCTGGAGCAGTAGGTCAGTTTGTTGGCCAATTTGCAACAGGTTGTGTTATGTTGTTGGAAGTGCTGGAAGCAAGGAAAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006950 GO:0006979 GO:0008150 GO:0016020 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N_2 ADH_zinc_N

Protein Analysis

225

Amino Acids

24.27

Weight (kDa)

5.67

Isoelectric Point (pI)

34.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N_2 PF16884 25 - 74 4.6e-08 N-terminal domain of oxidoreductase
ADH_N_2 PF16884 98 - 141 3.3e-10 N-terminal domain of oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000196)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65560
fragaria_vesca FvH4_1g00330 FvH4_1g00342 FvH4_1g00343 FvH4_1g00344 FvH4_1g00345 FvH4_1g00345 FvH4_1g00346 FvH4_1g26520
malus_domestica MD02G1001800.v1.1 MD02G1001900.v1.1 MD02G1002100.v1.1 MD02G1002300.v1.1 MD02G1002400.v1.1 MD02G1002500.v1.1 MD02G1002600.v1.1 MD15G1145700.v1.1
prunus_persica Prupe.7G268600_v2.0.a1 Prupe.7G268700_v2.0.a1 Prupe.7G268800_v2.0.a1 Prupe.7G268800_v2.0.a1 Prupe.7G268900_v2.0.a1
pyrus_communis pycom02g00050 pycom02g00060 pycom02g00100 pycom02g00120 pycom02g00130 pycom15g13090 pycom15g13100
rosa_chinensis RchiOBHm_Chr1g0325321 RchiOBHm_Chr1g0325341 RchiOBHm_Chr1g0325351 RchiOBHm_Chr1g0325361 RchiOBHm_Chr1g0364881 RchiOBHm_Chr1g0364891 RchiOBHm_Chr2g0084701 RchiOBHm_Chr2g0084721 RchiOBHm_Chr2g0084731 RchiOBHm_Chr2g0084741 RchiOBHm_Chr2g0084751 RchiOBHm_Chr2g0084761 RchiOBHm_Chr2g0084771 RchiOBHm_Chr2g0085311 RchiOBHm_Chr2g0085321 RchiOBHm_Chr2g0085341 RchiOBHm_Chr2g0085351 RchiOBHm_Chr2g0117721 RchiOBHm_Chr2g0159631 RchiOBHm_Chr3g0484011 RchiOBHm_Chr5g0021141 RchiOBHm_Chr5g0061081 RchiOBHm_Chr6g0275731 RchiOBHm_Chr7g0238011
rosa_laevigata RLG00000013438 RLG00000015632 RLG00000015634 RLG00000015635 RLG00000015636 RLG00000015637 RLG00000015677 RLG00000015679 RLG00000030145 RLG00000030146 RLG00000035427
rosa_multiflora Rmu_co8334849.1_g000001 Rmu_sc0001366.1_g000035 Rmu_sc0002352.1_g000003 Rmu_sc0002352.1_g000004 Rmu_sc0002352.1_g000006 Rmu_sc0002352.1_g000007 Rmu_sc0002352.1_g000009 Rmu_sc0002586.1_g000008 Rmu_sc0004966.1_g000016 Rmu_sc0007122.1_g000001 Rmu_sc0008804.1_g000002 Rmu_sc0008883.1_g000001 Rmu_sc0010860.1_g000002 Rmu_sc0012119.1_g000001 Rmu_sc0012119.1_g000003 Rmu_sc0012119.1_g000006 Rmu_sc0012119.1_g000007 Rmu_sc0012119.1_g000009 Rmu_sc0020031.1_g000004 Rmu_sc0020800.1_g000005 Rmu_sc0020800.1_g000007 Rmu_sc0020800.1_g000008 Rmu_sc0020800.1_g000009 Rmu_sc0025597.1_g000001 Rmu_sc0029132.1_g000002
rosa_roxburghii Rroxscaffold_2G00155450 Rroxscaffold_2G00155460 Rroxscaffold_2G00155470 Rroxscaffold_2G00155830 Rroxscaffold_2G00155840 Rroxscaffold_2G00155850 Rroxscaffold_2G00155860 Rroxscaffold_2G00155870 Rroxscaffold_2G00155910 Rroxscaffold_3G00273850 Rroxscaffold_4G00324820 Rroxscaffold_7G00192930 Rroxscaffold_7G00192960
rosa_rugosa Rorug01G0051300 Rorug01G0051400 Rorug01G0455700 Rorug01G0455800 Rorug01G0455900 Rorug01G0459500 Rorug01G0459500 Rorug01G0459600 Rorug01G0459700 Rorug01G0459700 Rorug02G0335000
rosa_samantha Rh1AG066500 Rh1BG054700 Rh1BG054800 Rh1BG176600 Rh2BG004400 Rh2BG004500 Rh2BG004600 Rh2BG004700 Rh2BG008600 Rh2BG008700 Rh2CG004500 Rh2CG004700 Rh2CG004800 Rh2CG004900 Rh2CG005000 Rh2CG005100 Rh2CG005200 Rh2CG009300 Rh2CG009400 Rh2DG004200 Rh2DG004300 Rh2DG004400 Rh2DG004500 Rh2DG004600 Rh2DG009800 Rh5BG411800 Rh5CG167200 Rh5CG436800 Rh5CG578800 Rh6DG203800
rosa_wichuraiana Rw1G005560 Rw2G000320 Rw2G000350 Rw2G000360 Rw2G000370 Rw2G000750 Rw2G000760 Rw4G022130 Rw5G015810 Rw5G037620 Rw6G018120 Rw7G038530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 264
AccI GTMKAC 2 cut(s) 467, 579
AciI CCGC 1 cut(s) 591
AclWI GGATC 3 cut(s) 179, 329, 342
AcoI YGGCCR 1 cut(s) 619
AfiI CCNNNNNNNGG 2 cut(s) 299, 566
AhdI GACNNNNNGTC 1 cut(s) 463
AjnI CCWGG 3 cut(s) 321, 349, 517
AleI CACNNNNGTG 1 cut(s) 433
AluBI AGCT 4 cut(s) 36, 65, 133, 201
AluI AGCT 4 cut(s) 36, 65, 133, 201
Alw26I GTCTC 2 cut(s) 414, 566
AlwI GGATC 3 cut(s) 179, 329, 342
AoxI GGCC 3 cut(s) 151, 490, 619
AsuHPI GGTGA 3 cut(s) 67, 239, 391
AsuNHI GCTAGC 1 cut(s) 32
BalI TGGCCA 1 cut(s) 621
BamHI GGATCC 1 cut(s) 334
BanII GRGCYC 1 cut(s) 485
BciT130I CCWGG 3 cut(s) 323, 351, 519
BcoDI GTCTC 2 cut(s) 414, 566
BfaI CTAG 2 cut(s) 33, 330
BglII AGATCT 1 cut(s) 549
BisI GCNGC 1 cut(s) 591
BlsI GCNGC 1 cut(s) 592
Bme1390I CCNGG 3 cut(s) 323, 351, 519
BmeRI GACNNNNNGTC 1 cut(s) 463
BmiI GGNNCC 1 cut(s) 336
BmrFI CCNGG 3 cut(s) 323, 351, 519
BmsI GCATC 1 cut(s) 602
BmtI GCTAGC 1 cut(s) 36
BplI GAGNNNNNCTC 2 cut(s) 539, 571
BpmI CTGGAG 2 cut(s) 540, 618
Bpu10I CCTNAGC 1 cut(s) 37
BpuEI CTTGAG 2 cut(s) 113, 258
BsaJI CCNNGG 1 cut(s) 350
BsaXI ACNNNNNCTCC 2 cut(s) 561, 591
Bsc4I CCNNNNNNNGG 2 cut(s) 299, 566
Bse118I RCCGGY 1 cut(s) 77
Bse1I ACTGG 4 cut(s) 154, 379, 459, 475
BseBI CCWGG 3 cut(s) 323, 351, 519
BseDI CCNNGG 1 cut(s) 350
BseLI CCNNNNNNNGG 2 cut(s) 299, 566
BseNI ACTGG 4 cut(s) 154, 379, 459, 475
BseRI GAGGAG 2 cut(s) 146, 245
BshFI GGCC 3 cut(s) 153, 492, 621
BsiSI CCGG 1 cut(s) 78
BslFI GGGAC 1 cut(s) 368
BslI CCNNNNNNNGG 2 cut(s) 299, 566
BsmAI GTCTC 2 cut(s) 414, 566
BsmFI GGGAC 1 cut(s) 368
BsnI GGCC 3 cut(s) 153, 492, 621
Bsp1286I GDGCHC 1 cut(s) 485
Bsp143I GATC 3 cut(s) 184, 334, 549
BspACI CCGC 1 cut(s) 591
BspANI GGCC 3 cut(s) 153, 492, 621
BspLI GGNNCC 1 cut(s) 336
BspOI GCTAGC 1 cut(s) 36
BspPI GGATC 3 cut(s) 179, 329, 342
BsrFI RCCGGY 1 cut(s) 77
BsrI ACTGG 4 cut(s) 154, 379, 459, 475
BssAI RCCGGY 1 cut(s) 77
BssECI CCNNGG 1 cut(s) 350
BssMI GATC 3 cut(s) 184, 334, 549
Bst2UI CCWGG 3 cut(s) 323, 351, 519
Bst4CI ACNGT 1 cut(s) 577
Bst6I CTCTTC 1 cut(s) 216
BstC8I GCNNGC 1 cut(s) 34
BstDEI CTNAG 2 cut(s) 37, 561
BstENI CCTNNNNNAGG 1 cut(s) 564
BstKTI GATC 3 cut(s) 187, 337, 552
BstMAI GTCTC 2 cut(s) 414, 566
BstMBI GATC 3 cut(s) 184, 334, 549
BstMWI GCNNNNNNNGC 2 cut(s) 489, 599
BstNI CCWGG 3 cut(s) 323, 351, 519
BstNSI RCATGY 1 cut(s) 196
BstSCI CCNGG 3 cut(s) 321, 349, 517
BstX2I RGATCY 2 cut(s) 334, 549
BstYI RGATCY 2 cut(s) 334, 549
BsuRI GGCC 3 cut(s) 153, 492, 621
BtsI GCAGTG 1 cut(s) 111
BtsIMutI CAGTG 3 cut(s) 111, 372, 428
Cac8I GCNNGC 1 cut(s) 34
Cfr10I RCCGGY 1 cut(s) 77
CviAII CATG 1 cut(s) 193
DdeI CTNAG 2 cut(s) 37, 561
DpnI GATC 3 cut(s) 186, 336, 551
DpnII GATC 3 cut(s) 184, 334, 549
DriI GACNNNNNGTC 1 cut(s) 463
EaeI YGGCCR 1 cut(s) 619
Eam1104I CTCTTC 1 cut(s) 216
Eam1105I GACNNNNNGTC 1 cut(s) 463
EarI CTCTTC 1 cut(s) 216
Eco24I GRGCYC 1 cut(s) 485
EcoNI CCTNNNNNAGG 1 cut(s) 564
EcoRII CCWGG 3 cut(s) 321, 349, 517
EcoT38I GRGCYC 1 cut(s) 485
FaeI CATG 1 cut(s) 196
FaqI GGGAC 1 cut(s) 368
FatI CATG 1 cut(s) 192
FauNDI CATATG 1 cut(s) 500
FblI GTMKAC 2 cut(s) 467, 579
Fnu4HI GCNGC 1 cut(s) 591
FriOI GRGCYC 1 cut(s) 485
Fsp4HI GCNGC 1 cut(s) 591
FspBI CTAG 2 cut(s) 33, 330
GluI GCNGC 1 cut(s) 591
GsuI CTGGAG 2 cut(s) 540, 618
HaeIII GGCC 3 cut(s) 153, 492, 621
HapII CCGG 1 cut(s) 78
Hin1II CATG 1 cut(s) 196
HindIII AAGCTT 1 cut(s) 131
HinfI GANTC 4 cut(s) 92, 326, 407, 421
HpaII CCGG 1 cut(s) 78
HphI GGTGA 3 cut(s) 67, 239, 391
Hpy166II GTNNAC 2 cut(s) 468, 580
Hpy188I TCNGA 2 cut(s) 97, 414
Hpy188III TCNNGA 3 cut(s) 137, 163, 597
Hpy8I GTNNAC 2 cut(s) 468, 580
HpyAV CCTTC 2 cut(s) 134, 211
HpyCH4III ACNGT 1 cut(s) 577
HpyCH4V TGCA 2 cut(s) 286, 629
HpyF10VI GCNNNNNNNGC 2 cut(s) 489, 599
HpyF3I CTNAG 2 cut(s) 37, 561
Hsp92II CATG 1 cut(s) 196
Kzo9I GATC 3 cut(s) 184, 334, 549
LmnI GCTCC 1 cut(s) 599
LweI GCATC 1 cut(s) 602
MaeI CTAG 2 cut(s) 33, 330
MaeIII GTNAC 2 cut(s) 368, 523
MalI GATC 3 cut(s) 186, 336, 551
MboI GATC 3 cut(s) 184, 334, 549
MboII GAAGA 2 cut(s) 233, 395
MflI RGATCY 2 cut(s) 334, 549
MhlI GDGCHC 1 cut(s) 485
MlsI TGGCCA 1 cut(s) 621
MluCI AATT 3 cut(s) 104, 297, 623
MluNI TGGCCA 1 cut(s) 621
MlyI GAGTC 1 cut(s) 416
MmeI TCCRAC 2 cut(s) 120, 630
MnlI CCTC 8 cut(s) 110, 164, 167, 179, 217, 223, 226, 449
Mox20I TGGCCA 1 cut(s) 621
MscI TGGCCA 1 cut(s) 621
MseI TTAA 2 cut(s) 345, 417
MslI CAYNNNNRTG 2 cut(s) 431, 433
Msp20I TGGCCA 1 cut(s) 621
MspI CCGG 1 cut(s) 78
MspR9I CCNGG 3 cut(s) 323, 351, 519
MvaI CCWGG 3 cut(s) 323, 351, 519
MwoI GCNNNNNNNGC 2 cut(s) 489, 599
NdeI CATATG 1 cut(s) 500
NdeII GATC 3 cut(s) 184, 334, 549
NheI GCTAGC 1 cut(s) 32
NlaIII CATG 1 cut(s) 196
NlaIV GGNNCC 1 cut(s) 336
NmuCI GTSAC 2 cut(s) 368, 523
NspI RCATGY 1 cut(s) 196
OliI CACNNNNGTG 1 cut(s) 433
PfeI GAWTC 3 cut(s) 92, 326, 421
PfoI TCCNGGA 1 cut(s) 321
PkrI GCNGC 1 cut(s) 592
PleI GAGTC 1 cut(s) 415
PpsI GAGTC 1 cut(s) 415
PsiI TTATAA 1 cut(s) 264
Psp6I CCWGG 3 cut(s) 321, 349, 517
PspGI CCWGG 3 cut(s) 321, 349, 517
PspN4I GGNNCC 1 cut(s) 336
PsuI RGATCY 2 cut(s) 334, 549
RseI CAYNNNNRTG 2 cut(s) 431, 433
SaqAI TTAA 2 cut(s) 345, 417
SatI GCNGC 1 cut(s) 591
Sau3AI GATC 3 cut(s) 184, 334, 549
SchI GAGTC 1 cut(s) 416
ScrFI CCNGG 3 cut(s) 323, 351, 519
SduI GDGCHC 1 cut(s) 485
SfaNI GCATC 1 cut(s) 602
SmiMI CAYNNNNRTG 2 cut(s) 431, 433
SmlI CTYRAG 2 cut(s) 128, 273
SmoI CTYRAG 2 cut(s) 128, 273
Sse9I AATT 3 cut(s) 104, 297, 623
SsiI CCGC 1 cut(s) 591
SspI AATATT 1 cut(s) 268
SspMI CTAG 2 cut(s) 33, 330
StyD4I CCNGG 3 cut(s) 321, 349, 517
TaaI ACNGT 1 cut(s) 577
TaqI TCGA 1 cut(s) 146
TasI AATT 3 cut(s) 104, 297, 623
TauI GCSGC 1 cut(s) 593
TfiI GAWTC 3 cut(s) 92, 326, 421
Tru1I TTAA 2 cut(s) 345, 417
Tru9I TTAA 2 cut(s) 345, 417
TscAI CASTG 3 cut(s) 118, 379, 435
TseFI GTSAC 2 cut(s) 368, 523
Tsp45I GTSAC 2 cut(s) 368, 523
TspDTI ATGAA 1 cut(s) 487
TspRI CASTG 3 cut(s) 118, 379, 435
XagI CCTNNNNNAGG 1 cut(s) 564
XceI RCATGY 1 cut(s) 196
XmiI GTMKAC 2 cut(s) 467, 579
XspI CTAG 2 cut(s) 33, 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.