Rw2G000320

2-alkenal reductase (NADP( )-dependent)-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000196
Physical Location & Seq
Forward (+)
315591 .. 317289
1699 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G000320.1

Sequence Viewer

Length: 1110 bp
ATGGCAGCTGCTGTTAATAGTAGTATCAATTATAAGGCAGGCGGTAATGGTGATGATCATGTTGAGCAAGTGAGGAACCAGCAAGTGCTAGCTAGAGACTACATAAGCGCCATTCCGGAGGAGTCAGACATGTACTTCAACACGTCTACCATTAAGCTCAAAGTGCCTGCAGGAGAAGAGTTCAAAGGATCTGTGCTGTTGAAGAACCTCTACCTCTCCTGCGATCCCTACACGCGAGGCGTTAAGCAAAGTGATTCCGCCACTACCAAACAAGACCGCATTGTCTTCTTCTCCCCTGACTCTCCAATAGTGGGTTATGGAGTGTCAAAAGTTGTGGATTCTCAGCATCCAGAGTTCAAGGCAGGGGACTTTGTTTGGGGTATAACAAAGTGGGAGGAGTATACTCTCATCACTAACATGGAAAGCTTCTTCAAAATCAACCACAATGATGTACCCCTTTCCTACTACACTGGACTTCTCGGAATGCCTGGTATGACTGCTTATGCTGGTTTCTATGAAGTCTGCAATCCAAAGAAAGGAGAGTATGTGTATATTTCCTCAGCATTTGGGGCTGTTGGTCAGATTGTCGGCCAATTTGCCAAACTTATGGGTTGTTATGTTGTTGGAAGTGCTGGCAGTCAAGAAAAGGTTGACAAACTAAAGAACAAGTTTGGATTTGATGAGGCATTCAATTACAAGGAAGAGCCCGACTTGAATGCTGCTTTGAAGAGGTACTTCCCAGAGGGCATAGACATTTACTTTGAGAATGTTGGAGGCAAAATGCTGGATGCAGTGCTGCTCAACATGAGACTTCATGGCCGTATTGCCGTGTGTGGCATGATTTCACAATACAATTACGAGCAACACGAAGGGATCACCAATTTGATGCACCTGATTTACAAGCGCATTCACATGGAAGGCTTCTTCGTGTTTGATTTCTACCACCTCTACCCCAAGTTCTTGGACCTGGTGGTGCCTTACATCAAGGAAGGGAAGATCACCTATGTGGAGGACATAGTTGAAGGACTTGAGAATGGTCCTGCTTCTCTAGTACGCCTTTTCAGTGGTCGCAATGCTGGAAAAGGAGTCGTTGCAGTTGCTCGCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N_2 ADH_zinc_N

Protein Analysis

369

Amino Acids

41.32

Weight (kDa)

5.71

Isoelectric Point (pI)

28.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N_2 PF16884 60 - 139 6.7e-17 N-terminal domain of oxidoreductase
ADH_zinc_N PF00107 191 - 324 4.9e-26 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000196)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65560
fragaria_vesca FvH4_1g00330 FvH4_1g00342 FvH4_1g00343 FvH4_1g00344 FvH4_1g00345 FvH4_1g00345 FvH4_1g00346 FvH4_1g26520
malus_domestica MD02G1001800.v1.1 MD02G1001900.v1.1 MD02G1002100.v1.1 MD02G1002300.v1.1 MD02G1002400.v1.1 MD02G1002500.v1.1 MD02G1002600.v1.1 MD15G1145700.v1.1
prunus_persica Prupe.7G268600_v2.0.a1 Prupe.7G268700_v2.0.a1 Prupe.7G268800_v2.0.a1 Prupe.7G268800_v2.0.a1 Prupe.7G268900_v2.0.a1
pyrus_communis pycom02g00050 pycom02g00060 pycom02g00100 pycom02g00120 pycom02g00130 pycom15g13090 pycom15g13100
rosa_chinensis RchiOBHm_Chr1g0325321 RchiOBHm_Chr1g0325341 RchiOBHm_Chr1g0325351 RchiOBHm_Chr1g0325361 RchiOBHm_Chr1g0364881 RchiOBHm_Chr1g0364891 RchiOBHm_Chr2g0084701 RchiOBHm_Chr2g0084721 RchiOBHm_Chr2g0084731 RchiOBHm_Chr2g0084741 RchiOBHm_Chr2g0084751 RchiOBHm_Chr2g0084761 RchiOBHm_Chr2g0084771 RchiOBHm_Chr2g0085311 RchiOBHm_Chr2g0085321 RchiOBHm_Chr2g0085341 RchiOBHm_Chr2g0085351 RchiOBHm_Chr2g0117721 RchiOBHm_Chr2g0159631 RchiOBHm_Chr3g0484011 RchiOBHm_Chr5g0021141 RchiOBHm_Chr5g0061081 RchiOBHm_Chr6g0275731 RchiOBHm_Chr7g0238011
rosa_laevigata RLG00000013438 RLG00000015632 RLG00000015634 RLG00000015635 RLG00000015636 RLG00000015637 RLG00000015677 RLG00000015679 RLG00000030145 RLG00000030146 RLG00000035427
rosa_multiflora Rmu_co8334849.1_g000001 Rmu_sc0001366.1_g000035 Rmu_sc0002352.1_g000003 Rmu_sc0002352.1_g000004 Rmu_sc0002352.1_g000006 Rmu_sc0002352.1_g000007 Rmu_sc0002352.1_g000009 Rmu_sc0002586.1_g000008 Rmu_sc0004966.1_g000016 Rmu_sc0007122.1_g000001 Rmu_sc0008804.1_g000002 Rmu_sc0008883.1_g000001 Rmu_sc0010860.1_g000002 Rmu_sc0012119.1_g000001 Rmu_sc0012119.1_g000003 Rmu_sc0012119.1_g000006 Rmu_sc0012119.1_g000007 Rmu_sc0012119.1_g000009 Rmu_sc0020031.1_g000004 Rmu_sc0020800.1_g000005 Rmu_sc0020800.1_g000007 Rmu_sc0020800.1_g000008 Rmu_sc0020800.1_g000009 Rmu_sc0025597.1_g000001 Rmu_sc0029132.1_g000002
rosa_roxburghii Rroxscaffold_2G00155450 Rroxscaffold_2G00155460 Rroxscaffold_2G00155470 Rroxscaffold_2G00155830 Rroxscaffold_2G00155840 Rroxscaffold_2G00155850 Rroxscaffold_2G00155860 Rroxscaffold_2G00155870 Rroxscaffold_2G00155910 Rroxscaffold_3G00273850 Rroxscaffold_4G00324820 Rroxscaffold_7G00192930 Rroxscaffold_7G00192960
rosa_rugosa Rorug01G0051300 Rorug01G0051400 Rorug01G0455700 Rorug01G0455800 Rorug01G0455900 Rorug01G0459500 Rorug01G0459500 Rorug01G0459600 Rorug01G0459700 Rorug01G0459700 Rorug02G0335000
rosa_samantha Rh1AG066500 Rh1BG054700 Rh1BG054800 Rh1BG176600 Rh2BG004400 Rh2BG004500 Rh2BG004600 Rh2BG004700 Rh2BG008600 Rh2BG008700 Rh2CG004500 Rh2CG004700 Rh2CG004800 Rh2CG004900 Rh2CG005000 Rh2CG005100 Rh2CG005200 Rh2CG009300 Rh2CG009400 Rh2DG004200 Rh2DG004300 Rh2DG004400 Rh2DG004500 Rh2DG004600 Rh2DG009800 Rh5BG411800 Rh5CG167200 Rh5CG436800 Rh5CG578800 Rh6DG203800
rosa_wichuraiana Rw1G005560 Rw2G000320 Rw2G000350 Rw2G000360 Rw2G000370 Rw2G000750 Rw2G000760 Rw4G022130 Rw5G015810 Rw5G037620 Rw6G018120 Rw7G038530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 33
AasI GACNNNNNNGTC 1 cut(s) 281
AccB1I GGYRCC 1 cut(s) 973
AccI GTMKAC 2 cut(s) 146, 401
AccII CGCG 2 cut(s) 235, 1104
AccIII TCCGGA 1 cut(s) 115
AciI CCGC 3 cut(s) 42, 258, 277
AclWI GGATC 3 cut(s) 196, 218, 881
AcoI YGGCCR 2 cut(s) 589, 817
AfaI GTAC 4 cut(s) 134, 453, 734, 1053
AfiI CCNNNNNNNGG 2 cut(s) 311, 536
AflIII ACRYGT 2 cut(s) 129, 141
AgsI TTSAA 9 cut(s) 139, 184, 202, 358, 433, 691, 715, 727, 1022
AjiI CACGTC 1 cut(s) 144
AjnI CCWGG 2 cut(s) 487, 966
AleI CACNNNNGTG 1 cut(s) 1004
AluBI AGCT 4 cut(s) 8, 92, 157, 426
AluI AGCT 4 cut(s) 8, 92, 157, 426
Alw26I GTCTC 2 cut(s) 90, 802
AlwI GGATC 3 cut(s) 196, 218, 881
AlwNI CAGNNNCTG 1 cut(s) 11
Aor13HI TCCGGA 1 cut(s) 115
AoxI GGCC 2 cut(s) 589, 817
ApeKI GCWGC 4 cut(s) 5, 8, 719, 796
ArsI GACNNNNNNTTYG 2 cut(s) 743, 775
AspLEI GCGC 2 cut(s) 110, 906
AspS9I GGNCC 2 cut(s) 964, 1037
AsuHPI GGTGA 3 cut(s) 62, 868, 991
AsuNHI GCTAGC 1 cut(s) 88
AvaII GGWCC 2 cut(s) 964, 1037
BanI GGYRCC 1 cut(s) 973
BanII GRGCYC 1 cut(s) 708
BarI GAAGNNNNNNTAC 2 cut(s) 194, 226
BbsI GAAGAC 1 cut(s) 277
BbvCI CCTCAGC 1 cut(s) 559
BbvI GCAGC 3 cut(s) 17, 706, 783
BceAI ACGGC 2 cut(s) 804, 812
BcgI CGANNNNNNTGC 2 cut(s) 698, 732
BciT130I CCWGG 2 cut(s) 489, 968
BclI TGATCA 1 cut(s) 55
BcoDI GTCTC 2 cut(s) 90, 802
BfaI CTAG 3 cut(s) 89, 93, 1049
BfmI CTRYAG 1 cut(s) 168
BfoI RGCGCY 1 cut(s) 111
BisI GCNGC 4 cut(s) 6, 9, 720, 797
BlsI GCNGC 4 cut(s) 7, 10, 721, 798
Bme1390I CCNGG 2 cut(s) 489, 968
Bme18I GGWCC 2 cut(s) 964, 1037
BmgBI CACGTC 1 cut(s) 144
BmgT120I GGNCC 2 cut(s) 964, 1037
BmiI GGNNCC 2 cut(s) 77, 975
BmrFI CCNGG 2 cut(s) 489, 968
BmsI GCATC 3 cut(s) 355, 778, 876
BmtI GCTAGC 1 cut(s) 92
BpiI GAAGAC 1 cut(s) 277
Bpu10I CCTNAGC 1 cut(s) 559
BpuEI CTTGAG 1 cut(s) 1049
BsaWI WCCGGW 1 cut(s) 115
BsaXI ACNNNNNCTCC 2 cut(s) 531, 561
Bsc4I CCNNNNNNNGG 2 cut(s) 311, 536
Bse1I ACTGG 1 cut(s) 475
Bse3DI GCAATG 1 cut(s) 1078
BseAI TCCGGA 1 cut(s) 115
BseBI CCWGG 2 cut(s) 489, 968
BseGI GGATG 2 cut(s) 346, 793
BseLI CCNNNNNNNGG 2 cut(s) 311, 536
BseMI GCAATG 1 cut(s) 1078
BseMII CTCAG 2 cut(s) 356, 573
BseNI ACTGG 1 cut(s) 475
BseRI GAGGAG 2 cut(s) 134, 410
BseXI GCAGC 3 cut(s) 17, 706, 783
Bsh1236I CGCG 2 cut(s) 235, 1104
BshFI GGCC 2 cut(s) 591, 819
BshNI GGYRCC 1 cut(s) 973
BsiSI CCGG 1 cut(s) 116
BslFI GGGAC 1 cut(s) 380
BslI CCNNNNNNNGG 2 cut(s) 311, 536
BsmAI GTCTC 2 cut(s) 90, 802
BsmFI GGGAC 1 cut(s) 380
BsmI GAATGC 4 cut(s) 489, 686, 721, 906
BsnI GGCC 2 cut(s) 591, 819
Bsp1286I GDGCHC 1 cut(s) 708
Bsp13I TCCGGA 1 cut(s) 115
Bsp143I GATC 5 cut(s) 55, 188, 223, 873, 996
Bsp68I TCGCGA 1 cut(s) 1104
BspACI CCGC 3 cut(s) 42, 258, 277
BspANI GGCC 2 cut(s) 591, 819
BspCNI CTCAG 2 cut(s) 355, 572
BspEI TCCGGA 1 cut(s) 115
BspFNI CGCG 2 cut(s) 235, 1104
BspLI GGNNCC 2 cut(s) 77, 975
BspMAI CTGCAG 1 cut(s) 172
BspOI GCTAGC 1 cut(s) 92
BspPI GGATC 3 cut(s) 196, 218, 881
BspQI GCTCTTC 1 cut(s) 696
BspT107I GGYRCC 1 cut(s) 973
BsrDI GCAATG 1 cut(s) 1078
BsrI ACTGG 1 cut(s) 475
BssMI GATC 5 cut(s) 55, 188, 223, 873, 996
BssNAI GTATAC 1 cut(s) 402
Bst1107I GTATAC 1 cut(s) 402
Bst2UI CCWGG 2 cut(s) 489, 968
Bst6I CTCTTC 3 cut(s) 171, 696, 722
BstC8I GCNNGC 5 cut(s) 40, 90, 168, 634, 1102
BstDEI CTNAG 2 cut(s) 342, 559
BstF5I GGATG 2 cut(s) 346, 793
BstFNI CGCG 2 cut(s) 235, 1104
BstH2I RGCGCY 1 cut(s) 111
BstHHI GCGC 2 cut(s) 110, 906
BstKTI GATC 5 cut(s) 58, 191, 226, 876, 999
BstMAI GTCTC 2 cut(s) 90, 802
BstMBI GATC 5 cut(s) 55, 188, 223, 873, 996
BstMWI GCNNNNNNNGC 2 cut(s) 163, 569
BstNI CCWGG 2 cut(s) 489, 968
BstNSI RCATGY 1 cut(s) 133
BstSCI CCNGG 2 cut(s) 487, 966
BstSFI CTRYAG 1 cut(s) 168
BstUI CGCG 2 cut(s) 235, 1104
BstV1I GCAGC 3 cut(s) 17, 706, 783
BstV2I GAAGAC 1 cut(s) 277
BstX2I RGATCY 1 cut(s) 188
BstXI CCANNNNNNTGG 2 cut(s) 607, 961
BstYI RGATCY 1 cut(s) 188
BstZ17I GTATAC 1 cut(s) 402
BsuRI GGCC 2 cut(s) 591, 819
BtrI CACGTC 1 cut(s) 144
BtsCI GGATG 2 cut(s) 346, 793
BtsI GCAGTG 1 cut(s) 798
BtsIMutI CAGTG 3 cut(s) 468, 798, 1069
BtuMI TCGCGA 1 cut(s) 1104
Cac8I GCNNGC 5 cut(s) 40, 90, 168, 634, 1102
CaiI CAGNNNCTG 1 cut(s) 11
CfoI GCGC 2 cut(s) 110, 906
Cfr13I GGNCC 2 cut(s) 964, 1037
CsiI ACCWGGT 1 cut(s) 966
Csp6I GTAC 4 cut(s) 133, 452, 733, 1052
CspCI CAANNNNNGTGG 2 cut(s) 315, 350
CviAII CATG 7 cut(s) 59, 130, 418, 805, 815, 838, 913
CviJI RGCY 9 cut(s) 8, 92, 157, 426, 572, 591, 706, 819, 921
CviKI_1 RGCY 9 cut(s) 8, 92, 157, 426, 572, 591, 706, 819, 921
CviQI GTAC 4 cut(s) 133, 452, 733, 1052
DdeI CTNAG 2 cut(s) 342, 559
DpnI GATC 5 cut(s) 57, 190, 225, 875, 998
DpnII GATC 5 cut(s) 55, 188, 223, 873, 996
DrdI GACNNNNNNGTC 1 cut(s) 281
DseDI GACNNNNNNGTC 1 cut(s) 281
EaeI YGGCCR 2 cut(s) 589, 817
Eam1104I CTCTTC 3 cut(s) 171, 696, 722
EarI CTCTTC 3 cut(s) 171, 696, 722
EciI GGCGGA 1 cut(s) 247
Eco24I GRGCYC 1 cut(s) 708
Eco47I GGWCC 2 cut(s) 964, 1037
EcoRII CCWGG 2 cut(s) 487, 966
EcoT38I GRGCYC 1 cut(s) 708
FaeI CATG 7 cut(s) 62, 133, 421, 808, 818, 841, 916
FalI AAGNNNNNCTT 2 cut(s) 719, 751
FaqI GGGAC 1 cut(s) 380
FatI CATG 7 cut(s) 58, 129, 417, 804, 814, 837, 912
FbaI TGATCA 1 cut(s) 55
FblI GTMKAC 2 cut(s) 146, 401
Fnu4HI GCNGC 4 cut(s) 6, 9, 720, 797
FokI GGATG 2 cut(s) 333, 800
FriOI GRGCYC 1 cut(s) 708
Fsp4HI GCNGC 4 cut(s) 6, 9, 720, 797
FspBI CTAG 3 cut(s) 89, 93, 1049
GlaI GCGC 2 cut(s) 109, 905
GluI GCNGC 4 cut(s) 6, 9, 720, 797
HaeII RGCGCY 1 cut(s) 111
HaeIII GGCC 2 cut(s) 591, 819
HapII CCGG 1 cut(s) 116
HhaI GCGC 2 cut(s) 110, 906
Hin1II CATG 7 cut(s) 62, 133, 421, 808, 818, 841, 916
Hin6I GCGC 2 cut(s) 108, 904
HinP1I GCGC 2 cut(s) 108, 904
HincII GTYRAC 1 cut(s) 652
HindII GTYRAC 1 cut(s) 652
HindIII AAGCTT 1 cut(s) 424
HinfI GANTC 5 cut(s) 122, 254, 299, 338, 1086
HpaII CCGG 1 cut(s) 116
HphI GGTGA 3 cut(s) 62, 868, 991
Hpy166II GTNNAC 3 cut(s) 147, 402, 652
Hpy188I TCNGA 3 cut(s) 127, 482, 582
Hpy188III TCNNGA 4 cut(s) 116, 350, 641, 1103
Hpy8I GTNNAC 3 cut(s) 147, 402, 652
HpyAV CCTTC 4 cut(s) 863, 911, 983, 1016
HpyCH4IV ACGT 1 cut(s) 143
HpyCH4V TGCA 5 cut(s) 170, 525, 791, 889, 1094
HpyF10VI GCNNNNNNNGC 2 cut(s) 163, 569
HpyF3I CTNAG 2 cut(s) 342, 559
HpySE526I ACGT 1 cut(s) 143
Hsp92II CATG 7 cut(s) 62, 133, 421, 808, 818, 841, 916
HspAI GCGC 2 cut(s) 108, 904
Kpn2I TCCGGA 1 cut(s) 115
Ksp22I TGATCA 1 cut(s) 55
Kzo9I GATC 5 cut(s) 55, 188, 223, 873, 996
LguI GCTCTTC 1 cut(s) 696
Lsp1109I GCAGC 3 cut(s) 17, 706, 783
LweI GCATC 3 cut(s) 355, 778, 876
MabI ACCWGGT 1 cut(s) 966
MaeI CTAG 3 cut(s) 89, 93, 1049
MaeII ACGT 1 cut(s) 143
MalI GATC 5 cut(s) 57, 190, 225, 875, 998
MboI GATC 5 cut(s) 55, 188, 223, 873, 996
MboII GAAGA 9 cut(s) 188, 214, 277, 280, 421, 713, 739, 916, 1006
MflI RGATCY 1 cut(s) 188
MhlI GDGCHC 1 cut(s) 708
MluCI AATT 5 cut(s) 28, 593, 691, 853, 880
MlyI GAGTC 3 cut(s) 131, 293, 1095
MmeI TCCRAC 2 cut(s) 604, 751
MroI TCCGGA 1 cut(s) 115
MseI TTAA 3 cut(s) 15, 153, 243
MslI CAYNNNNRTG 4 cut(s) 416, 447, 911, 1004
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 1 cut(s) 116
MspR9I CCNGG 2 cut(s) 489, 968
Mva1269I GAATGC 4 cut(s) 489, 686, 721, 906
MvaI CCWGG 2 cut(s) 489, 968
MvnI CGCG 2 cut(s) 235, 1104
MwoI GCNNNNNNNGC 2 cut(s) 163, 569
NdeII GATC 5 cut(s) 55, 188, 223, 873, 996
NheI GCTAGC 1 cut(s) 88
NlaIII CATG 7 cut(s) 62, 133, 421, 808, 818, 841, 916
NlaIV GGNNCC 2 cut(s) 77, 975
NruI TCGCGA 1 cut(s) 1104
NspI RCATGY 1 cut(s) 133
OliI CACNNNNGTG 1 cut(s) 1004
PciI ACATGT 1 cut(s) 129
PciSI GCTCTTC 1 cut(s) 696
PcsI WCGNNNNNNNCGW 1 cut(s) 864
PctI GAATGC 4 cut(s) 489, 686, 721, 906
PfeI GAWTC 2 cut(s) 254, 338
PkrI GCNGC 4 cut(s) 7, 10, 721, 798
PleI GAGTC 3 cut(s) 130, 293, 1094
PpsI GAGTC 3 cut(s) 130, 293, 1094
PscI ACATGT 1 cut(s) 129
PsiI TTATAA 1 cut(s) 33
Psp6I CCWGG 2 cut(s) 487, 966
PspGI CCWGG 2 cut(s) 487, 966
PspN4I GGNNCC 2 cut(s) 77, 975
PspPI GGNCC 2 cut(s) 964, 1037
PstI CTGCAG 1 cut(s) 172
PstNI CAGNNNCTG 1 cut(s) 11
PsuI RGATCY 1 cut(s) 188
PvuII CAGCTG 1 cut(s) 8
RruI TCGCGA 1 cut(s) 1104
RsaI GTAC 4 cut(s) 134, 453, 734, 1053
RsaNI GTAC 4 cut(s) 133, 452, 733, 1052
RseI CAYNNNNRTG 4 cut(s) 416, 447, 911, 1004
SapI GCTCTTC 1 cut(s) 696
SaqAI TTAA 3 cut(s) 15, 153, 243
SatI GCNGC 4 cut(s) 6, 9, 720, 797
Sau3AI GATC 5 cut(s) 55, 188, 223, 873, 996
Sau96I GGNCC 2 cut(s) 964, 1037
SbfI CCTGCAGG 1 cut(s) 172
SchI GAGTC 3 cut(s) 131, 293, 1095
ScrFI CCNGG 2 cut(s) 489, 968
SdaI CCTGCAGG 1 cut(s) 172
SduI GDGCHC 1 cut(s) 708
SexAI ACCWGGT 1 cut(s) 966
SfaNI GCATC 3 cut(s) 355, 778, 876
SfcI CTRYAG 1 cut(s) 168
SinI GGWCC 2 cut(s) 964, 1037
SmiMI CAYNNNNRTG 4 cut(s) 416, 447, 911, 1004
SmlI CTYRAG 1 cut(s) 1028
SmoI CTYRAG 1 cut(s) 1028
Sse8387I CCTGCAGG 1 cut(s) 172
Sse9I AATT 5 cut(s) 28, 593, 691, 853, 880
SsiI CCGC 3 cut(s) 42, 258, 277
SspMI CTAG 3 cut(s) 89, 93, 1049
StyD4I CCNGG 2 cut(s) 487, 966
TaiI ACGT 1 cut(s) 146
TasI AATT 5 cut(s) 28, 593, 691, 853, 880
TatI WGTACW 1 cut(s) 132
TfiI GAWTC 2 cut(s) 254, 338
Tru1I TTAA 3 cut(s) 15, 153, 243
Tru9I TTAA 3 cut(s) 15, 153, 243
TscAI CASTG 3 cut(s) 475, 798, 1069
TseI GCWGC 4 cut(s) 5, 8, 719, 796
TspDTI ATGAA 2 cut(s) 531, 803
TspRI CASTG 3 cut(s) 475, 798, 1069
VpaK11BI GGWCC 2 cut(s) 964, 1037
XceI RCATGY 1 cut(s) 133
XmiI GTMKAC 2 cut(s) 146, 401
XspI CTAG 3 cut(s) 89, 93, 1049
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.