Rw2G000360

2-alkenal reductase (NADP( )-dependent)-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000196
Physical Location & Seq
Forward (+)
338470 .. 340526
2057 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G000360.1

Sequence Viewer

Length: 1038 bp
ATGGCTAGTGGAGAAAAAGTGAGGAACAAGCAGGTGATATTCGGAGACTATATCACCGGCTTCCCCAAAGAATCCGACCTCAAATTGACCACTGGCACTACCAAACTCAAGCTTCCAGAAGGTTCGACTGGCCTCCTCGTCAAGAACCTCTACTTGTCCTGCGATCCGTACATGCGAAGCCGTATGACCAAGCACGACAGGCCCACTTATGTCCAATCCTTCACGCTCGGCTCGCCTGTAACTGGTTATGGAGTGGCTAAAGTCCTGGAATCTGGGGATCCAAAGTTTAAGCCAGGCGACTTGGTTTGGGGTCACACTGGTTGGGAAGAATATAGTGTCATCACCACAGAGTCTCTGATTAAGATTCACCACACTGATGTGCCTCTCTCTTACTATACTGGACTTCTCGGTATGCCTGGAATGACTGCTTATGCTGGTTTTTATGAGATCTGCTCTCCTAAGAAAGAAGAGACGGTCTACATTTCAGCCGCATCTGGAGCAGTAGGTCAGCTTGTTGGCCAATTTGCAAAGTTAACAGGTTGCTATGTTGTTGGGAGTGCTGGAAGCAAGGAAAAGGTTGATCTGCTGAAGAACAAATTTGGATTTGACGAGGCTTTCAACTATAAAGACGAACCTGACCTAGATGCAGCTTTAAGAAGGTACTTTCCAGATGGTATTGACATTTACTTTGAAAATGTGGGGGGAAAGATGCTTGATGCAGTGCTACCAAACATGAGGCTGAAAGGGCGAATAGCTGTGTGTGGGATGATCTCACAGTACAACCTCGAGCGGCCTGAAGGCATACATAATTTGATGTCTCTGATTGTTAAGCAGGTCCGAATGGAAGGTTTCCTGGTCTTCAGTTACTATCATCTGTACGGAAAGTTTCTTGAAACAGTGTTGCCTTACATAAAAGAAGGGAAGATAACATATGTGGAAGATGTAGTTGAAGGCCTTGAGAATGCTCCAGCGGCTCTAATTGGGCTCTTTTCTGGCCGCAATGTGGGAAAGCAGGTGGTTGTAGTTTCCAGAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006950 GO:0006979 GO:0008150 GO:0016020 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N_2 ADH_zinc_N

Protein Analysis

345

Amino Acids

38.21

Weight (kDa)

7.01

Isoelectric Point (pI)

32.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N_2 PF16884 10 - 122 7.3e-27 N-terminal domain of oxidoreductase
ADH_zinc_N PF00107 167 - 291 3.2e-23 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 201 - 340 6.8e-10 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000196)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65560
fragaria_vesca FvH4_1g00330 FvH4_1g00342 FvH4_1g00343 FvH4_1g00344 FvH4_1g00345 FvH4_1g00345 FvH4_1g00346 FvH4_1g26520
malus_domestica MD02G1001800.v1.1 MD02G1001900.v1.1 MD02G1002100.v1.1 MD02G1002300.v1.1 MD02G1002400.v1.1 MD02G1002500.v1.1 MD02G1002600.v1.1 MD15G1145700.v1.1
prunus_persica Prupe.7G268600_v2.0.a1 Prupe.7G268700_v2.0.a1 Prupe.7G268800_v2.0.a1 Prupe.7G268800_v2.0.a1 Prupe.7G268900_v2.0.a1
pyrus_communis pycom02g00050 pycom02g00060 pycom02g00100 pycom02g00120 pycom02g00130 pycom15g13090 pycom15g13100
rosa_chinensis RchiOBHm_Chr1g0325321 RchiOBHm_Chr1g0325341 RchiOBHm_Chr1g0325351 RchiOBHm_Chr1g0325361 RchiOBHm_Chr1g0364881 RchiOBHm_Chr1g0364891 RchiOBHm_Chr2g0084701 RchiOBHm_Chr2g0084721 RchiOBHm_Chr2g0084731 RchiOBHm_Chr2g0084741 RchiOBHm_Chr2g0084751 RchiOBHm_Chr2g0084761 RchiOBHm_Chr2g0084771 RchiOBHm_Chr2g0085311 RchiOBHm_Chr2g0085321 RchiOBHm_Chr2g0085341 RchiOBHm_Chr2g0085351 RchiOBHm_Chr2g0117721 RchiOBHm_Chr2g0159631 RchiOBHm_Chr3g0484011 RchiOBHm_Chr5g0021141 RchiOBHm_Chr5g0061081 RchiOBHm_Chr6g0275731 RchiOBHm_Chr7g0238011
rosa_laevigata RLG00000013438 RLG00000015632 RLG00000015634 RLG00000015635 RLG00000015636 RLG00000015637 RLG00000015677 RLG00000015679 RLG00000030145 RLG00000030146 RLG00000035427
rosa_multiflora Rmu_co8334849.1_g000001 Rmu_sc0001366.1_g000035 Rmu_sc0002352.1_g000003 Rmu_sc0002352.1_g000004 Rmu_sc0002352.1_g000006 Rmu_sc0002352.1_g000007 Rmu_sc0002352.1_g000009 Rmu_sc0002586.1_g000008 Rmu_sc0004966.1_g000016 Rmu_sc0007122.1_g000001 Rmu_sc0008804.1_g000002 Rmu_sc0008883.1_g000001 Rmu_sc0010860.1_g000002 Rmu_sc0012119.1_g000001 Rmu_sc0012119.1_g000003 Rmu_sc0012119.1_g000006 Rmu_sc0012119.1_g000007 Rmu_sc0012119.1_g000009 Rmu_sc0020031.1_g000004 Rmu_sc0020800.1_g000005 Rmu_sc0020800.1_g000007 Rmu_sc0020800.1_g000008 Rmu_sc0020800.1_g000009 Rmu_sc0025597.1_g000001 Rmu_sc0029132.1_g000002
rosa_roxburghii Rroxscaffold_2G00155450 Rroxscaffold_2G00155460 Rroxscaffold_2G00155470 Rroxscaffold_2G00155830 Rroxscaffold_2G00155840 Rroxscaffold_2G00155850 Rroxscaffold_2G00155860 Rroxscaffold_2G00155870 Rroxscaffold_2G00155910 Rroxscaffold_3G00273850 Rroxscaffold_4G00324820 Rroxscaffold_7G00192930 Rroxscaffold_7G00192960
rosa_rugosa Rorug01G0051300 Rorug01G0051400 Rorug01G0455700 Rorug01G0455800 Rorug01G0455900 Rorug01G0459500 Rorug01G0459500 Rorug01G0459600 Rorug01G0459700 Rorug01G0459700 Rorug02G0335000
rosa_samantha Rh1AG066500 Rh1BG054700 Rh1BG054800 Rh1BG176600 Rh2BG004400 Rh2BG004500 Rh2BG004600 Rh2BG004700 Rh2BG008600 Rh2BG008700 Rh2CG004500 Rh2CG004700 Rh2CG004800 Rh2CG004900 Rh2CG005000 Rh2CG005100 Rh2CG005200 Rh2CG009300 Rh2CG009400 Rh2DG004200 Rh2DG004300 Rh2DG004400 Rh2DG004500 Rh2DG004600 Rh2DG009800 Rh5BG411800 Rh5CG167200 Rh5CG436800 Rh5CG578800 Rh6DG203800
rosa_wichuraiana Rw1G005560 Rw2G000320 Rw2G000350 Rw2G000360 Rw2G000370 Rw2G000750 Rw2G000760 Rw4G022130 Rw5G015810 Rw5G037620 Rw6G018120 Rw7G038530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 22, 1003
Acc36I ACCTGC 3 cut(s) 22, 823, 1003
AccBSI CCGCTC 1 cut(s) 790
AccI GTMKAC 1 cut(s) 477
AciI CCGC 4 cut(s) 489, 790, 971, 997
AclWI GGATC 3 cut(s) 158, 272, 285
AcoI YGGCCR 2 cut(s) 517, 994
AcsI RAATTY 1 cut(s) 596
AcuI CTGAAG 3 cut(s) 608, 816, 844
AfaI GTAC 4 cut(s) 170, 662, 779, 878
AfiI CCNNNNNNNGG 2 cut(s) 242, 1003
AgsI TTSAA 4 cut(s) 619, 692, 893, 950
AjnI CCWGG 4 cut(s) 264, 292, 415, 852
AleI CACNNNNGTG 1 cut(s) 377
AluBI AGCT 4 cut(s) 112, 511, 650, 755
AluI AGCT 4 cut(s) 112, 511, 650, 755
Alw26I GTCTC 4 cut(s) 39, 357, 464, 822
AlwI GGATC 3 cut(s) 158, 272, 285
Ama87I CYCGRG 1 cut(s) 785
AoxI GGCC 6 cut(s) 130, 200, 517, 791, 952, 994
ApeKI GCWGC 1 cut(s) 647
ApoI RAATTY 1 cut(s) 596
ArsI GACNNNNNNTTYG 2 cut(s) 671, 703
AspS9I GGNCC 2 cut(s) 201, 835
AsuHPI GGTGA 4 cut(s) 46, 46, 334, 359
AvaI CYCGRG 1 cut(s) 785
AvaII GGWCC 1 cut(s) 835
BalI TGGCCA 1 cut(s) 519
BamHI GGATCC 1 cut(s) 277
BanII GRGCYC 1 cut(s) 987
BbsI GAAGAC 1 cut(s) 850
BbvI GCAGC 1 cut(s) 659
BccI CCATC 1 cut(s) 665
BceAI ACGGC 1 cut(s) 165
BciT130I CCWGG 4 cut(s) 266, 294, 417, 854
BcoDI GTCTC 4 cut(s) 39, 357, 464, 822
BfaI CTAG 2 cut(s) 6, 641
BfuAI ACCTGC 3 cut(s) 22, 823, 1003
BglII AGATCT 1 cut(s) 447
BisI GCNGC 5 cut(s) 489, 648, 791, 972, 997
BlsI GCNGC 5 cut(s) 490, 649, 792, 973, 998
Bme1390I CCNGG 4 cut(s) 266, 294, 417, 854
Bme18I GGWCC 1 cut(s) 835
BmeT110I CYCGRG 1 cut(s) 785
BmgT120I GGNCC 2 cut(s) 201, 835
BmiI GGNNCC 1 cut(s) 279
BmrFI CCNGG 4 cut(s) 266, 294, 417, 854
BmsI GCATC 4 cut(s) 500, 634, 699, 706
BpiI GAAGAC 1 cut(s) 850
BplI GAGNNNNNCTC 2 cut(s) 437, 469
BpmI CTGGAG 2 cut(s) 516, 951
BpuEI CTTGAG 2 cut(s) 92, 977
BsaXI ACNNNNNCTCC 1 cut(s) 33
Bsc4I CCNNNNNNNGG 2 cut(s) 242, 1003
Bse118I RCCGGY 1 cut(s) 56
Bse1I ACTGG 5 cut(s) 97, 133, 247, 322, 403
Bse3DI GCAATG 1 cut(s) 1006
BseBI CCWGG 4 cut(s) 266, 294, 417, 854
BseGI GGATG 1 cut(s) 771
BseLI CCNNNNNNNGG 2 cut(s) 242, 1003
BseMI GCAATG 1 cut(s) 1006
BseNI ACTGG 5 cut(s) 97, 133, 247, 322, 403
BseRI GAGGAG 1 cut(s) 125
BseXI GCAGC 1 cut(s) 659
BshFI GGCC 6 cut(s) 132, 202, 519, 793, 954, 996
BsiHKCI CYCGRG 1 cut(s) 785
BsiSI CCGG 1 cut(s) 57
BslI CCNNNNNNNGG 2 cut(s) 242, 1003
BsmAI GTCTC 4 cut(s) 39, 357, 464, 822
BsmBI CGTCTC 1 cut(s) 464
BsmI GAATGC 1 cut(s) 967
BsnI GGCC 6 cut(s) 132, 202, 519, 793, 954, 996
BsoBI CYCGRG 1 cut(s) 785
Bsp1286I GDGCHC 1 cut(s) 987
Bsp143I GATC 5 cut(s) 163, 277, 447, 580, 768
BspACI CCGC 4 cut(s) 489, 790, 971, 997
BspANI GGCC 6 cut(s) 132, 202, 519, 793, 954, 996
BspLI GGNNCC 1 cut(s) 279
BspMI ACCTGC 3 cut(s) 22, 823, 1003
BspPI GGATC 3 cut(s) 158, 272, 285
BsrBI CCGCTC 1 cut(s) 790
BsrDI GCAATG 1 cut(s) 1006
BsrFI RCCGGY 1 cut(s) 56
BsrI ACTGG 5 cut(s) 97, 133, 247, 322, 403
BssAI RCCGGY 1 cut(s) 56
BssMI GATC 5 cut(s) 163, 277, 447, 580, 768
Bst2UI CCWGG 4 cut(s) 266, 294, 417, 854
Bst4CI ACNGT 3 cut(s) 475, 777, 898
Bst6I CTCTTC 1 cut(s) 462
BstC8I GCNNGC 1 cut(s) 233
BstDEI CTNAG 1 cut(s) 459
BstF5I GGATG 1 cut(s) 771
BstKTI GATC 5 cut(s) 166, 280, 450, 583, 771
BstMAI GTCTC 4 cut(s) 39, 357, 464, 822
BstMBI GATC 5 cut(s) 163, 277, 447, 580, 768
BstMWI GCNNNNNNNGC 5 cut(s) 199, 232, 497, 745, 971
BstNI CCWGG 4 cut(s) 266, 294, 417, 854
BstNSI RCATGY 1 cut(s) 175
BstSCI CCNGG 4 cut(s) 264, 292, 415, 852
BstV1I GCAGC 1 cut(s) 659
BstV2I GAAGAC 1 cut(s) 850
BstX2I RGATCY 2 cut(s) 277, 447
BstYI RGATCY 2 cut(s) 277, 447
BsuRI GGCC 6 cut(s) 132, 202, 519, 793, 954, 996
BtsCI GGATG 1 cut(s) 771
BtsI GCAGTG 1 cut(s) 726
BtsIMutI CAGTG 5 cut(s) 90, 315, 372, 726, 903
BveI ACCTGC 3 cut(s) 22, 823, 1003
Cac8I GCNNGC 1 cut(s) 233
Cfr10I RCCGGY 1 cut(s) 56
Cfr13I GGNCC 2 cut(s) 201, 835
Csp6I GTAC 4 cut(s) 169, 661, 778, 877
CviAII CATG 2 cut(s) 172, 733
CviQI GTAC 4 cut(s) 169, 661, 778, 877
DdeI CTNAG 1 cut(s) 459
DpnI GATC 5 cut(s) 165, 279, 449, 582, 770
DpnII GATC 5 cut(s) 163, 277, 447, 580, 768
EaeI YGGCCR 2 cut(s) 517, 994
Eam1104I CTCTTC 1 cut(s) 462
EarI CTCTTC 1 cut(s) 462
Eco147I AGGCCT 1 cut(s) 954
Eco24I GRGCYC 1 cut(s) 987
Eco47I GGWCC 1 cut(s) 835
Eco57I CTGAAG 3 cut(s) 608, 816, 844
Eco88I CYCGRG 1 cut(s) 785
EcoRII CCWGG 4 cut(s) 264, 292, 415, 852
EcoT38I GRGCYC 1 cut(s) 987
Esp3I CGTCTC 1 cut(s) 464
FaeI CATG 2 cut(s) 175, 736
FatI CATG 2 cut(s) 171, 732
FauNDI CATATG 1 cut(s) 931
FblI GTMKAC 1 cut(s) 477
Fnu4HI GCNGC 5 cut(s) 489, 648, 791, 972, 997
FokI GGATG 1 cut(s) 778
FriOI GRGCYC 1 cut(s) 987
Fsp4HI GCNGC 5 cut(s) 489, 648, 791, 972, 997
FspBI CTAG 2 cut(s) 6, 641
GluI GCNGC 5 cut(s) 489, 648, 791, 972, 997
GsuI CTGGAG 2 cut(s) 516, 951
HaeIII GGCC 6 cut(s) 132, 202, 519, 793, 954, 996
HapII CCGG 1 cut(s) 57
Hin1II CATG 2 cut(s) 175, 736
HincII GTYRAC 1 cut(s) 534
HindII GTYRAC 1 cut(s) 534
HindIII AAGCTT 1 cut(s) 110
HinfI GANTC 4 cut(s) 71, 269, 350, 364
HpaI GTTAAC 1 cut(s) 534
HpaII CCGG 1 cut(s) 57
HphI GGTGA 4 cut(s) 46, 46, 334, 359
Hpy166II GTNNAC 2 cut(s) 478, 534
Hpy188I TCNGA 5 cut(s) 44, 76, 357, 822, 839
Hpy188III TCNNGA 6 cut(s) 116, 142, 495, 668, 890, 1029
Hpy8I GTNNAC 2 cut(s) 478, 534
HpyAV CCTTC 7 cut(s) 113, 229, 651, 791, 839, 911, 944
HpyCH4III ACNGT 3 cut(s) 475, 777, 898
HpyCH4V TGCA 3 cut(s) 527, 647, 719
HpyF10VI GCNNNNNNNGC 5 cut(s) 199, 232, 497, 745, 971
HpyF3I CTNAG 1 cut(s) 459
Hsp92II CATG 2 cut(s) 175, 736
KspAI GTTAAC 1 cut(s) 534
Kzo9I GATC 5 cut(s) 163, 277, 447, 580, 768
LmnI GCTCC 2 cut(s) 497, 970
Lsp1109I GCAGC 1 cut(s) 659
LweI GCATC 4 cut(s) 500, 634, 699, 706
MaeI CTAG 2 cut(s) 6, 641
MaeIII GTNAC 3 cut(s) 238, 311, 863
MalI GATC 5 cut(s) 165, 279, 449, 582, 770
MbiI CCGCTC 1 cut(s) 790
MboI GATC 5 cut(s) 163, 277, 447, 580, 768
MboII GAAGA 6 cut(s) 338, 479, 601, 850, 934, 950
MflI RGATCY 2 cut(s) 277, 447
MhlI GDGCHC 1 cut(s) 987
MlsI TGGCCA 1 cut(s) 519
MluCI AATT 5 cut(s) 83, 521, 596, 808, 978
MluNI TGGCCA 1 cut(s) 519
MlyI GAGTC 1 cut(s) 359
MmeI TCCRAC 1 cut(s) 99
MnlI CCTC 9 cut(s) 15, 89, 143, 146, 158, 393, 604, 729, 794
Mox20I TGGCCA 1 cut(s) 519
MscI TGGCCA 1 cut(s) 519
MseI TTAA 5 cut(s) 288, 360, 533, 653, 828
MslI CAYNNNNRTG 2 cut(s) 375, 377
Msp20I TGGCCA 1 cut(s) 519
MspA1I CMGCKG 1 cut(s) 971
MspI CCGG 1 cut(s) 57
MspR9I CCNGG 4 cut(s) 266, 294, 417, 854
Mva1269I GAATGC 1 cut(s) 967
MvaI CCWGG 4 cut(s) 266, 294, 417, 854
MwoI GCNNNNNNNGC 5 cut(s) 199, 232, 497, 745, 971
NdeI CATATG 1 cut(s) 931
NdeII GATC 5 cut(s) 163, 277, 447, 580, 768
NlaIII CATG 2 cut(s) 175, 736
NlaIV GGNNCC 1 cut(s) 279
NmeAIII GCCGAG 1 cut(s) 207
NmuCI GTSAC 1 cut(s) 311
NspI RCATGY 1 cut(s) 175
OliI CACNNNNGTG 1 cut(s) 377
PaeR7I CTCGAG 1 cut(s) 785
PaqCI CACCTGC 2 cut(s) 22, 1003
PceI AGGCCT 1 cut(s) 954
PctI GAATGC 1 cut(s) 967
PfeI GAWTC 3 cut(s) 71, 269, 364
PfoI TCCNGGA 1 cut(s) 264
PkrI GCNGC 5 cut(s) 490, 649, 792, 973, 998
PleI GAGTC 1 cut(s) 358
PpsI GAGTC 1 cut(s) 358
Psp6I CCWGG 4 cut(s) 264, 292, 415, 852
PspGI CCWGG 4 cut(s) 264, 292, 415, 852
PspN4I GGNNCC 1 cut(s) 279
PspPI GGNCC 2 cut(s) 201, 835
PspXI VCTCGAGB 1 cut(s) 785
PsuI RGATCY 2 cut(s) 277, 447
RsaI GTAC 4 cut(s) 170, 662, 779, 878
RsaNI GTAC 4 cut(s) 169, 661, 778, 877
RseI CAYNNNNRTG 2 cut(s) 375, 377
SaqAI TTAA 5 cut(s) 288, 360, 533, 653, 828
SatI GCNGC 5 cut(s) 489, 648, 791, 972, 997
Sau3AI GATC 5 cut(s) 163, 277, 447, 580, 768
Sau96I GGNCC 2 cut(s) 201, 835
SchI GAGTC 1 cut(s) 359
ScrFI CCNGG 4 cut(s) 266, 294, 417, 854
SduI GDGCHC 1 cut(s) 987
SfaNI GCATC 4 cut(s) 500, 634, 699, 706
Sfr274I CTCGAG 1 cut(s) 785
SinI GGWCC 1 cut(s) 835
SlaI CTCGAG 1 cut(s) 785
SmiMI CAYNNNNRTG 2 cut(s) 375, 377
SmlI CTYRAG 3 cut(s) 107, 785, 956
SmoI CTYRAG 3 cut(s) 107, 785, 956
Sse9I AATT 5 cut(s) 83, 521, 596, 808, 978
SseBI AGGCCT 1 cut(s) 954
SsiI CCGC 4 cut(s) 489, 790, 971, 997
SspMI CTAG 2 cut(s) 6, 641
StuI AGGCCT 1 cut(s) 954
StyD4I CCNGG 4 cut(s) 264, 292, 415, 852
TaaI ACNGT 3 cut(s) 475, 777, 898
TaqI TCGA 2 cut(s) 125, 786
TasI AATT 5 cut(s) 83, 521, 596, 808, 978
TatI WGTACW 1 cut(s) 777
TauI GCSGC 4 cut(s) 491, 793, 974, 999
TfiI GAWTC 3 cut(s) 71, 269, 364
Tru1I TTAA 5 cut(s) 288, 360, 533, 653, 828
Tru9I TTAA 5 cut(s) 288, 360, 533, 653, 828
TscAI CASTG 5 cut(s) 97, 322, 379, 726, 903
TseFI GTSAC 1 cut(s) 311
TseI GCWGC 1 cut(s) 647
Tsp45I GTSAC 1 cut(s) 311
TspGWI ACGGA 2 cut(s) 156, 894
TspRI CASTG 5 cut(s) 97, 322, 379, 726, 903
VpaK11BI GGWCC 1 cut(s) 835
XapI RAATTY 1 cut(s) 596
XceI RCATGY 1 cut(s) 175
XhoI CTCGAG 1 cut(s) 785
XmiI GTMKAC 1 cut(s) 477
XspI CTAG 2 cut(s) 6, 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.