Rw6G018120

2-alkenal reductase (NADP( )-dependent)-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000196
Physical Location & Seq
Reverse (-)
35870508 .. 35872116
1609 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G018120.1

Sequence Viewer

Length: 798 bp
ATGTTGTTGAAGAACCTCTACCTCTCCTGCGATCTCTACATGCGAGGCATTAAGCACAGTGATTCCTCCACTACCAAACAAGACCGCATTGTCTTCTTCTCCCCTGAGTCTCCAATAGTGGGCTATGGAGTGTCAAAAGCTGTGGATTCTCAACAACCAGAGCTCAAGGCAGGGGACTTGGTTTTTTGGGGTATAACAAAGTGGGAGGAGTATACTTTCATCACTAAGACGGAAAGCTTGTTCAAAATCAACCACACTGATGTACCCCTTTCCTACTACACTGGACTTCTCAGACTGCCTGGTATGACTGCTTATGCTAGTTTCTACAAAGTCTACAATCCAAAGAAAGGAGAGTATGTGTACATTTCCTCAGCATTCGGGGCTGTTGGTCAGATTGTTGGACAATTTGCCAAACTCATGGGTTGTTATATTGTTGGAAGTGCTGGCAGTCAGGAAAATGTTGACATACTGAAGAACAAGTTTGGATTTGATGAGGCATTCAATTACAAGGAAGAGCCTGACTTGGATGCTGCTTTGAAGAGGTACTTCCCAAAGGGCATAGACATTTACTTCGAGAATGTTGAAGGCAAAATGCTGGATGCAGTGCTGCTCAACATGAGACTTCACTGCCGATTGCAGTCAACACGAAGGGGTCACCAACGTGATGCCTCTGATTTAAAAGCTCATAGCATGGAAGGCTTCTTCATGTTTGATTTCTACCACCTCTATCCTAAGTTCTTGGACCTGGTGGTGCCTTACATCAAGGAAAGGAAGATCGCCTATGTGGAGGACATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N_2 ADH_zinc_N

Protein Analysis

265

Amino Acids

30.45

Weight (kDa)

7.01

Isoelectric Point (pI)

35.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N_2 PF16884 1 - 82 5.6e-10 N-terminal domain of oxidoreductase
ADH_zinc_N PF00107 128 - 211 3.6e-15 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000196)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65560
fragaria_vesca FvH4_1g00330 FvH4_1g00342 FvH4_1g00343 FvH4_1g00344 FvH4_1g00345 FvH4_1g00345 FvH4_1g00346 FvH4_1g26520
malus_domestica MD02G1001800.v1.1 MD02G1001900.v1.1 MD02G1002100.v1.1 MD02G1002300.v1.1 MD02G1002400.v1.1 MD02G1002500.v1.1 MD02G1002600.v1.1 MD15G1145700.v1.1
prunus_persica Prupe.7G268600_v2.0.a1 Prupe.7G268700_v2.0.a1 Prupe.7G268800_v2.0.a1 Prupe.7G268800_v2.0.a1 Prupe.7G268900_v2.0.a1
pyrus_communis pycom02g00050 pycom02g00060 pycom02g00100 pycom02g00120 pycom02g00130 pycom15g13090 pycom15g13100
rosa_chinensis RchiOBHm_Chr1g0325321 RchiOBHm_Chr1g0325341 RchiOBHm_Chr1g0325351 RchiOBHm_Chr1g0325361 RchiOBHm_Chr1g0364881 RchiOBHm_Chr1g0364891 RchiOBHm_Chr2g0084701 RchiOBHm_Chr2g0084721 RchiOBHm_Chr2g0084731 RchiOBHm_Chr2g0084741 RchiOBHm_Chr2g0084751 RchiOBHm_Chr2g0084761 RchiOBHm_Chr2g0084771 RchiOBHm_Chr2g0085311 RchiOBHm_Chr2g0085321 RchiOBHm_Chr2g0085341 RchiOBHm_Chr2g0085351 RchiOBHm_Chr2g0117721 RchiOBHm_Chr2g0159631 RchiOBHm_Chr3g0484011 RchiOBHm_Chr5g0021141 RchiOBHm_Chr5g0061081 RchiOBHm_Chr6g0275731 RchiOBHm_Chr7g0238011
rosa_laevigata RLG00000013438 RLG00000015632 RLG00000015634 RLG00000015635 RLG00000015636 RLG00000015637 RLG00000015677 RLG00000015679 RLG00000030145 RLG00000030146 RLG00000035427
rosa_multiflora Rmu_co8334849.1_g000001 Rmu_sc0001366.1_g000035 Rmu_sc0002352.1_g000003 Rmu_sc0002352.1_g000004 Rmu_sc0002352.1_g000006 Rmu_sc0002352.1_g000007 Rmu_sc0002352.1_g000009 Rmu_sc0002586.1_g000008 Rmu_sc0004966.1_g000016 Rmu_sc0007122.1_g000001 Rmu_sc0008804.1_g000002 Rmu_sc0008883.1_g000001 Rmu_sc0010860.1_g000002 Rmu_sc0012119.1_g000001 Rmu_sc0012119.1_g000003 Rmu_sc0012119.1_g000006 Rmu_sc0012119.1_g000007 Rmu_sc0012119.1_g000009 Rmu_sc0020031.1_g000004 Rmu_sc0020800.1_g000005 Rmu_sc0020800.1_g000007 Rmu_sc0020800.1_g000008 Rmu_sc0020800.1_g000009 Rmu_sc0025597.1_g000001 Rmu_sc0029132.1_g000002
rosa_roxburghii Rroxscaffold_2G00155450 Rroxscaffold_2G00155460 Rroxscaffold_2G00155470 Rroxscaffold_2G00155830 Rroxscaffold_2G00155840 Rroxscaffold_2G00155850 Rroxscaffold_2G00155860 Rroxscaffold_2G00155870 Rroxscaffold_2G00155910 Rroxscaffold_3G00273850 Rroxscaffold_4G00324820 Rroxscaffold_7G00192930 Rroxscaffold_7G00192960
rosa_rugosa Rorug01G0051300 Rorug01G0051400 Rorug01G0455700 Rorug01G0455800 Rorug01G0455900 Rorug01G0459500 Rorug01G0459500 Rorug01G0459600 Rorug01G0459700 Rorug01G0459700 Rorug02G0335000
rosa_samantha Rh1AG066500 Rh1BG054700 Rh1BG054800 Rh1BG176600 Rh2BG004400 Rh2BG004500 Rh2BG004600 Rh2BG004700 Rh2BG008600 Rh2BG008700 Rh2CG004500 Rh2CG004700 Rh2CG004800 Rh2CG004900 Rh2CG005000 Rh2CG005100 Rh2CG005200 Rh2CG009300 Rh2CG009400 Rh2DG004200 Rh2DG004300 Rh2DG004400 Rh2DG004500 Rh2DG004600 Rh2DG009800 Rh5BG411800 Rh5CG167200 Rh5CG436800 Rh5CG578800 Rh6DG203800
rosa_wichuraiana Rw1G005560 Rw2G000320 Rw2G000350 Rw2G000360 Rw2G000370 Rw2G000750 Rw2G000760 Rw4G022130 Rw5G015810 Rw5G037620 Rw6G018120 Rw7G038530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 89
AccB1I GGYRCC 1 cut(s) 751
AccI GTMKAC 2 cut(s) 212, 333
AciI CCGC 1 cut(s) 85
AcuI CTGAAG 1 cut(s) 491
AfaI GTAC 3 cut(s) 264, 362, 545
AfiI CCNNNNNNNGG 2 cut(s) 119, 347
AgsI TTSAA 5 cut(s) 10, 244, 502, 538, 584
AjnI CCWGG 2 cut(s) 298, 744
AleI CACNNNNGTG 1 cut(s) 660
AloI GAACNNNNNNTCC 2 cut(s) 224, 256
AluBI AGCT 4 cut(s) 140, 163, 237, 683
AluI AGCT 4 cut(s) 140, 163, 237, 683
Alw21I GWGCWC 1 cut(s) 165
Alw26I GTCTC 2 cut(s) 114, 613
ApeKI GCWGC 2 cut(s) 530, 607
ArsI GACNNNNNNTTYG 2 cut(s) 554, 586
AspS9I GGNCC 1 cut(s) 742
AsuHPI GGTGA 1 cut(s) 647
AvaII GGWCC 1 cut(s) 742
BaeI ACNNNNGTAYC 2 cut(s) 246, 279
BanI GGYRCC 1 cut(s) 751
BanII GRGCYC 1 cut(s) 165
BarI GAAGNNNNNNTAC 1 cut(s) 34
BbsI GAAGAC 1 cut(s) 85
Bbv12I GWGCWC 1 cut(s) 165
BbvCI CCTCAGC 1 cut(s) 370
BbvI GCAGC 2 cut(s) 517, 594
BciT130I CCWGG 2 cut(s) 300, 746
BcoDI GTCTC 2 cut(s) 114, 613
BfaI CTAG 1 cut(s) 318
BisI GCNGC 2 cut(s) 531, 608
BlsI GCNGC 2 cut(s) 532, 609
Bme1390I CCNGG 2 cut(s) 300, 746
Bme18I GGWCC 1 cut(s) 742
BmgT120I GGNCC 1 cut(s) 742
BmiI GGNNCC 1 cut(s) 753
BmrFI CCNGG 2 cut(s) 300, 746
BmsI GCATC 3 cut(s) 517, 589, 655
BpiI GAAGAC 1 cut(s) 85
Bpu10I CCTNAGC 1 cut(s) 370
BpuEI CTTGAG 1 cut(s) 149
BsaXI ACNNNNNCTCC 2 cut(s) 342, 372
Bsc4I CCNNNNNNNGG 2 cut(s) 119, 347
Bse1I ACTGG 1 cut(s) 286
BseBI CCWGG 2 cut(s) 300, 746
BseGI GGATG 2 cut(s) 532, 604
BseLI CCNNNNNNNGG 2 cut(s) 119, 347
BseMII CTCAG 3 cut(s) 96, 304, 384
BseNI ACTGG 1 cut(s) 286
BseRI GAGGAG 1 cut(s) 221
BseXI GCAGC 2 cut(s) 517, 594
BshNI GGYRCC 1 cut(s) 751
BsiHKAI GWGCWC 1 cut(s) 165
BslFI GGGAC 1 cut(s) 188
BslI CCNNNNNNNGG 2 cut(s) 119, 347
BsmAI GTCTC 2 cut(s) 114, 613
BsmFI GGGAC 1 cut(s) 188
BsmI GAATGC 2 cut(s) 374, 497
Bsp1286I GDGCHC 1 cut(s) 165
Bsp1407I TGTACA 1 cut(s) 360
Bsp143I GATC 2 cut(s) 31, 774
BspACI CCGC 1 cut(s) 85
BspCNI CTCAG 3 cut(s) 97, 303, 383
BspLI GGNNCC 1 cut(s) 753
BspQI GCTCTTC 1 cut(s) 507
BspT107I GGYRCC 1 cut(s) 751
BsrGI TGTACA 1 cut(s) 360
BsrI ACTGG 1 cut(s) 286
BssMI GATC 2 cut(s) 31, 774
BssNAI GTATAC 1 cut(s) 213
Bst1107I GTATAC 1 cut(s) 213
Bst2UI CCWGG 2 cut(s) 300, 746
Bst4CI ACNGT 1 cut(s) 59
Bst6I CTCTTC 2 cut(s) 507, 533
BstAUI TGTACA 1 cut(s) 360
BstC8I GCNNGC 1 cut(s) 445
BstDEI CTNAG 5 cut(s) 105, 225, 290, 370, 732
BstEII GGTNACC 1 cut(s) 653
BstF5I GGATG 2 cut(s) 532, 604
BstKTI GATC 2 cut(s) 34, 777
BstMAI GTCTC 2 cut(s) 114, 613
BstMBI GATC 2 cut(s) 31, 774
BstMWI GCNNNNNNNGC 2 cut(s) 380, 696
BstNI CCWGG 2 cut(s) 300, 746
BstNSI RCATGY 1 cut(s) 43
BstPI GGTNACC 1 cut(s) 653
BstSCI CCNGG 2 cut(s) 298, 744
BstV1I GCAGC 2 cut(s) 517, 594
BstV2I GAAGAC 1 cut(s) 85
BstXI CCANNNNNNTGG 1 cut(s) 418
BstZ17I GTATAC 1 cut(s) 213
BtsCI GGATG 2 cut(s) 532, 604
BtsI GCAGTG 2 cut(s) 609, 625
BtsIMutI CAGTG 5 cut(s) 64, 255, 279, 609, 625
Cac8I GCNNGC 1 cut(s) 445
Cfr13I GGNCC 1 cut(s) 742
CsiI ACCWGGT 1 cut(s) 744
Csp6I GTAC 3 cut(s) 263, 361, 544
CspCI CAANNNNNGTGG 2 cut(s) 123, 158
CviAII CATG 5 cut(s) 40, 418, 616, 691, 706
CviJI RGCY 8 cut(s) 123, 140, 163, 237, 383, 517, 683, 699
CviKI_1 RGCY 8 cut(s) 123, 140, 163, 237, 383, 517, 683, 699
CviQI GTAC 3 cut(s) 263, 361, 544
DdeI CTNAG 5 cut(s) 105, 225, 290, 370, 732
DpnI GATC 2 cut(s) 33, 776
DpnII GATC 2 cut(s) 31, 774
DraI TTTAAA 1 cut(s) 678
DrdI GACNNNNNNGTC 1 cut(s) 89
DseDI GACNNNNNNGTC 1 cut(s) 89
Eam1104I CTCTTC 2 cut(s) 507, 533
EarI CTCTTC 2 cut(s) 507, 533
Ecl136II GAGCTC 1 cut(s) 163
Eco24I GRGCYC 1 cut(s) 165
Eco47I GGWCC 1 cut(s) 742
Eco53kI GAGCTC 1 cut(s) 163
Eco57I CTGAAG 1 cut(s) 491
Eco91I GGTNACC 1 cut(s) 653
EcoICRI GAGCTC 1 cut(s) 163
EcoO65I GGTNACC 1 cut(s) 653
EcoRII CCWGG 2 cut(s) 298, 744
EcoT38I GRGCYC 1 cut(s) 165
FaeI CATG 5 cut(s) 43, 421, 619, 694, 709
FalI AAGNNNNNCTT 2 cut(s) 530, 562
FaqI GGGAC 1 cut(s) 188
FatI CATG 5 cut(s) 39, 417, 615, 690, 705
FblI GTMKAC 2 cut(s) 212, 333
Fnu4HI GCNGC 2 cut(s) 531, 608
FokI GGATG 2 cut(s) 539, 611
FriOI GRGCYC 1 cut(s) 165
Fsp4HI GCNGC 2 cut(s) 531, 608
FspBI CTAG 1 cut(s) 318
GluI GCNGC 2 cut(s) 531, 608
Hin1II CATG 5 cut(s) 43, 421, 619, 694, 709
HincII GTYRAC 2 cut(s) 463, 642
HindII GTYRAC 2 cut(s) 463, 642
HindIII AAGCTT 1 cut(s) 235
HinfI GANTC 3 cut(s) 62, 107, 146
HphI GGTGA 1 cut(s) 647
Hpy166II GTNNAC 5 cut(s) 213, 334, 361, 463, 642
Hpy188I TCNGA 3 cut(s) 293, 393, 673
Hpy188III TCNNGA 2 cut(s) 452, 574
Hpy8I GTNNAC 5 cut(s) 213, 334, 361, 463, 642
HpyAV CCTTC 3 cut(s) 578, 642, 689
HpyCH4III ACNGT 1 cut(s) 59
HpyCH4IV ACGT 1 cut(s) 661
HpyCH4V TGCA 2 cut(s) 602, 637
HpyF10VI GCNNNNNNNGC 2 cut(s) 380, 696
HpyF3I CTNAG 5 cut(s) 105, 225, 290, 370, 732
HpySE526I ACGT 1 cut(s) 661
Hsp92II CATG 5 cut(s) 43, 421, 619, 694, 709
Kzo9I GATC 2 cut(s) 31, 774
LguI GCTCTTC 1 cut(s) 507
Lsp1109I GCAGC 2 cut(s) 517, 594
LweI GCATC 3 cut(s) 517, 589, 655
MabI ACCWGGT 1 cut(s) 744
MaeI CTAG 1 cut(s) 318
MaeII ACGT 1 cut(s) 661
MaeIII GTNAC 1 cut(s) 653
MalI GATC 2 cut(s) 33, 776
MboI GATC 2 cut(s) 31, 774
MboII GAAGA 8 cut(s) 22, 85, 88, 484, 524, 550, 694, 784
MhlI GDGCHC 1 cut(s) 165
MluCI AATT 2 cut(s) 404, 502
MlyI GAGTC 1 cut(s) 116
MmeI TCCRAC 2 cut(s) 379, 415
MseI TTAA 2 cut(s) 51, 677
MslI CAYNNNNRTG 2 cut(s) 258, 660
MspR9I CCNGG 2 cut(s) 300, 746
Mva1269I GAATGC 2 cut(s) 374, 497
MvaI CCWGG 2 cut(s) 300, 746
MwoI GCNNNNNNNGC 2 cut(s) 380, 696
NdeII GATC 2 cut(s) 31, 774
NlaIII CATG 5 cut(s) 43, 421, 619, 694, 709
NlaIV GGNNCC 1 cut(s) 753
NmuCI GTSAC 1 cut(s) 653
NspI RCATGY 1 cut(s) 43
OliI CACNNNNGTG 1 cut(s) 660
PciSI GCTCTTC 1 cut(s) 507
PctI GAATGC 2 cut(s) 374, 497
PfeI GAWTC 2 cut(s) 62, 146
PkrI GCNGC 2 cut(s) 532, 609
PleI GAGTC 1 cut(s) 115
PpsI GAGTC 1 cut(s) 115
Psp124BI GAGCTC 1 cut(s) 165
Psp6I CCWGG 2 cut(s) 298, 744
PspEI GGTNACC 1 cut(s) 653
PspGI CCWGG 2 cut(s) 298, 744
PspN4I GGNNCC 1 cut(s) 753
PspPI GGNCC 1 cut(s) 742
RsaI GTAC 3 cut(s) 264, 362, 545
RsaNI GTAC 3 cut(s) 263, 361, 544
RseI CAYNNNNRTG 2 cut(s) 258, 660
SacI GAGCTC 1 cut(s) 165
SapI GCTCTTC 1 cut(s) 507
SaqAI TTAA 2 cut(s) 51, 677
SatI GCNGC 2 cut(s) 531, 608
Sau3AI GATC 2 cut(s) 31, 774
Sau96I GGNCC 1 cut(s) 742
SchI GAGTC 1 cut(s) 116
ScrFI CCNGG 2 cut(s) 300, 746
SduI GDGCHC 1 cut(s) 165
SexAI ACCWGGT 1 cut(s) 744
SfaNI GCATC 3 cut(s) 517, 589, 655
SinI GGWCC 1 cut(s) 742
SmiMI CAYNNNNRTG 2 cut(s) 258, 660
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
Sse9I AATT 2 cut(s) 404, 502
SsiI CCGC 1 cut(s) 85
SspMI CTAG 1 cut(s) 318
SstI GAGCTC 1 cut(s) 165
StyD4I CCNGG 2 cut(s) 298, 744
TaaI ACNGT 1 cut(s) 59
TaiI ACGT 1 cut(s) 664
TaqI TCGA 1 cut(s) 573
TasI AATT 2 cut(s) 404, 502
TatI WGTACW 1 cut(s) 360
TfiI GAWTC 2 cut(s) 62, 146
Tru1I TTAA 2 cut(s) 51, 677
Tru9I TTAA 2 cut(s) 51, 677
TscAI CASTG 5 cut(s) 64, 262, 286, 609, 632
TseFI GTSAC 1 cut(s) 653
TseI GCWGC 2 cut(s) 530, 607
Tsp45I GTSAC 1 cut(s) 653
TspDTI ATGAA 2 cut(s) 208, 694
TspGWI ACGGA 1 cut(s) 245
TspRI CASTG 5 cut(s) 64, 262, 286, 609, 632
VpaK11BI GGWCC 1 cut(s) 742
XceI RCATGY 1 cut(s) 43
XmiI GTMKAC 2 cut(s) 212, 333
XspI CTAG 1 cut(s) 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.