MD02G1001800.v1.1

2-alkenal reductase (NADP( )-dependent)-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000196
Physical Location & Seq
Reverse (-)
154735 .. 157440
2706 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1001800.v1.1.491

Sequence Viewer

Length: 1212 bp
ATGGCCTACGTGCAGTCTACCCCCTATAAACCACCGCTTCCGAATCCATCGACTTGGAATCAACCATTGAGGCAGCAGCAGCACTGCTCATTTCGCCCTCCGCCTACAGAGAGAGTTAGTAAAAAGAGAGACAAGGAAAAGAAAACAGCAATAATCGATCCAATGGCGGCGAGTACAGAGGGAGTGATCAGCAACAAGCAGGTGCTACTGAAATACTATGTGACCGGCTTCCCCAAAGAATCTGACATGCAACTCATCACCGCCACCACCAAGCTCAAGCTTCCAGAAGGTTCCATAGGGGTTCTGGTGAAGAACCTCTACTTGTCCTGCGACCCTTATACGAGATTCCGCATGACCAAGCCTGAACCTGGCGCCGGTTATGTCGATTCCTTCAAACCCGGTTCGCCTATAGGCGGATATGGAGTTGCCAAAGTTTTGGAATCCGGGGATCCCAAGTTTAAGCCAGGCGACTTCATTTGGGGAATGACCGGGTGGGAAGAGTACAGTGTTATCACTGCAACAGAGTCTTTGTTTAAGATTCAGCACACTGATGTGCCTCTCTCTTACTATACTGGAATTCTTGGTATGCCTGGAATGACTGCTTATGCTGGTTTTTATGAGGTCTGCAATCCTAAGAAGGGAGAGACGGTCTTCGTTTCAGCAGCATCTGGAGCAGTAGGTCAGCTTGTTGGCCAATTTGCAAAGTTGTTGGGTTGCTATGTTGTTGGAAGTGCCGGAAGCAAAGAAAAGGTTGATCTGTTGAATAACAAGTTGGGGTTTGACAATGCTTTCAATTATAAAGAAGAGCCTGATTTGGATGCAGCTTTAAAAAGATATTTTCCTGAGGGTATTGACATATACTTTGAAAATGTCGGGGGAAAGATGCTTGATGCAGTGCTACAAAACATGAGGGTCAATGGCCGGATTGCAGTTTGTGGGATGATATCGCAGTACACCCTTGACCAGCGGGAAGGTTGTCACAATTTAATATATCTGGTCAGTAAACAGGTTCGCATGCAAGGTTTCACGGTTCTTAGTTACTACCATCTTTACGGGAAGTTTCTTGAAATGGTTCTGCCGGCCATAAAAGAAGGGAAGATAACTTATGTGGAAGATGTAGTGGAAGGCCTTGAGAGCGCTCCAGCTGCTCTGGTAGGGCTCTTTGCCGGCCGCAATGTAGGAAAGCAGGTGGTTGCAGTTTCCCGAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006950 GO:0006979 GO:0008150 GO:0016020 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N_2 ADH_zinc_N

Protein Analysis

404

Amino Acids

44.44

Weight (kDa)

8.63

Isoelectric Point (pI)

30.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N_2 PF16884 66 - 175 4e-25 N-terminal domain of oxidoreductase
ADH_zinc_N PF00107 225 - 356 2.1e-23 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 258 - 398 4.6e-09 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000196)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65560
fragaria_vesca FvH4_1g00330 FvH4_1g00342 FvH4_1g00343 FvH4_1g00344 FvH4_1g00345 FvH4_1g00345 FvH4_1g00346 FvH4_1g26520
malus_domestica MD02G1001800.v1.1 MD02G1001900.v1.1 MD02G1002100.v1.1 MD02G1002300.v1.1 MD02G1002400.v1.1 MD02G1002500.v1.1 MD02G1002600.v1.1 MD15G1145700.v1.1
prunus_persica Prupe.7G268600_v2.0.a1 Prupe.7G268700_v2.0.a1 Prupe.7G268800_v2.0.a1 Prupe.7G268800_v2.0.a1 Prupe.7G268900_v2.0.a1
pyrus_communis pycom02g00050 pycom02g00060 pycom02g00100 pycom02g00120 pycom02g00130 pycom15g13090 pycom15g13100
rosa_chinensis RchiOBHm_Chr1g0325321 RchiOBHm_Chr1g0325341 RchiOBHm_Chr1g0325351 RchiOBHm_Chr1g0325361 RchiOBHm_Chr1g0364881 RchiOBHm_Chr1g0364891 RchiOBHm_Chr2g0084701 RchiOBHm_Chr2g0084721 RchiOBHm_Chr2g0084731 RchiOBHm_Chr2g0084741 RchiOBHm_Chr2g0084751 RchiOBHm_Chr2g0084761 RchiOBHm_Chr2g0084771 RchiOBHm_Chr2g0085311 RchiOBHm_Chr2g0085321 RchiOBHm_Chr2g0085341 RchiOBHm_Chr2g0085351 RchiOBHm_Chr2g0117721 RchiOBHm_Chr2g0159631 RchiOBHm_Chr3g0484011 RchiOBHm_Chr5g0021141 RchiOBHm_Chr5g0061081 RchiOBHm_Chr6g0275731 RchiOBHm_Chr7g0238011
rosa_laevigata RLG00000013438 RLG00000015632 RLG00000015634 RLG00000015635 RLG00000015636 RLG00000015637 RLG00000015677 RLG00000015679 RLG00000030145 RLG00000030146 RLG00000035427
rosa_multiflora Rmu_co8334849.1_g000001 Rmu_sc0001366.1_g000035 Rmu_sc0002352.1_g000003 Rmu_sc0002352.1_g000004 Rmu_sc0002352.1_g000006 Rmu_sc0002352.1_g000007 Rmu_sc0002352.1_g000009 Rmu_sc0002586.1_g000008 Rmu_sc0004966.1_g000016 Rmu_sc0007122.1_g000001 Rmu_sc0008804.1_g000002 Rmu_sc0008883.1_g000001 Rmu_sc0010860.1_g000002 Rmu_sc0012119.1_g000001 Rmu_sc0012119.1_g000003 Rmu_sc0012119.1_g000006 Rmu_sc0012119.1_g000007 Rmu_sc0012119.1_g000009 Rmu_sc0020031.1_g000004 Rmu_sc0020800.1_g000005 Rmu_sc0020800.1_g000007 Rmu_sc0020800.1_g000008 Rmu_sc0020800.1_g000009 Rmu_sc0025597.1_g000001 Rmu_sc0029132.1_g000002
rosa_roxburghii Rroxscaffold_2G00155450 Rroxscaffold_2G00155460 Rroxscaffold_2G00155470 Rroxscaffold_2G00155830 Rroxscaffold_2G00155840 Rroxscaffold_2G00155850 Rroxscaffold_2G00155860 Rroxscaffold_2G00155870 Rroxscaffold_2G00155910 Rroxscaffold_3G00273850 Rroxscaffold_4G00324820 Rroxscaffold_7G00192930 Rroxscaffold_7G00192960
rosa_rugosa Rorug01G0051300 Rorug01G0051400 Rorug01G0455700 Rorug01G0455800 Rorug01G0455900 Rorug01G0459500 Rorug01G0459500 Rorug01G0459600 Rorug01G0459700 Rorug01G0459700 Rorug02G0335000
rosa_samantha Rh1AG066500 Rh1BG054700 Rh1BG054800 Rh1BG176600 Rh2BG004400 Rh2BG004500 Rh2BG004600 Rh2BG004700 Rh2BG008600 Rh2BG008700 Rh2CG004500 Rh2CG004700 Rh2CG004800 Rh2CG004900 Rh2CG005000 Rh2CG005100 Rh2CG005200 Rh2CG009300 Rh2CG009400 Rh2DG004200 Rh2DG004300 Rh2DG004400 Rh2DG004500 Rh2DG004600 Rh2DG009800 Rh5BG411800 Rh5CG167200 Rh5CG436800 Rh5CG578800 Rh6DG203800
rosa_wichuraiana Rw1G005560 Rw2G000320 Rw2G000350 Rw2G000360 Rw2G000370 Rw2G000750 Rw2G000760 Rw4G022130 Rw5G015810 Rw5G037620 Rw6G018120 Rw7G038530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 798
AarI CACCTGC 2 cut(s) 190, 1177
Acc36I ACCTGC 2 cut(s) 190, 1177
AccB1I GGYRCC 1 cut(s) 371
AccI GTMKAC 1 cut(s) 17
AciI CCGC 8 cut(s) 35, 101, 167, 261, 349, 414, 967, 1171
AclWI GGATC 3 cut(s) 152, 443, 456
AcoI YGGCCR 4 cut(s) 691, 919, 1080, 1168
AcsI RAATTY 1 cut(s) 576
AcyI GRCGYC 1 cut(s) 372
AfaI GTAC 3 cut(s) 175, 503, 953
AfeI AGCGCT 1 cut(s) 1138
AfiI CCNNNNNNNGG 4 cut(s) 368, 374, 413, 638
AgsI TTSAA 5 cut(s) 394, 763, 793, 866, 1067
AjnI CCWGG 3 cut(s) 367, 463, 589
AjuI GAANNNNNNNTTGG 2 cut(s) 755, 787
AleI CACNNNNGTG 1 cut(s) 551
AluBI AGCT 5 cut(s) 274, 280, 685, 824, 1145
AluI AGCT 5 cut(s) 274, 280, 685, 824, 1145
Alw26I GTCTC 2 cut(s) 123, 638
AlwI GGATC 3 cut(s) 152, 443, 456
AlwNI CAGNNNCTG 1 cut(s) 668
Ama87I CYCGRG 1 cut(s) 1203
Aor51HI AGCGCT 1 cut(s) 1138
AoxI GGCC 6 cut(s) 3, 691, 919, 1080, 1126, 1168
ApeKI GCWGC 6 cut(s) 73, 76, 79, 662, 821, 1145
ApoI RAATTY 1 cut(s) 576
ArsI GACNNNNNNTTYG 2 cut(s) 845, 877
Asp700I GAANNNNTTC 1 cut(s) 1071
AspLEI GCGC 2 cut(s) 374, 1139
AsuC2I CCSGG 3 cut(s) 399, 445, 490
AsuHPI GGTGA 2 cut(s) 250, 319
AvaI CYCGRG 1 cut(s) 1203
AxyI CCTNAGG 1 cut(s) 843
BalI TGGCCA 1 cut(s) 693
BamHI GGATCC 1 cut(s) 448
BanI GGYRCC 1 cut(s) 371
BanII GRGCYC 1 cut(s) 1161
BarI GAAGNNNNNNTAC 2 cut(s) 302, 334
BbsI GAAGAC 1 cut(s) 643
BbvI GCAGC 6 cut(s) 85, 88, 91, 674, 833, 1132
BccI CCATC 2 cut(s) 55, 1053
BciT130I CCWGG 3 cut(s) 369, 465, 591
BclI TGATCA 1 cut(s) 186
BcnI CCSGG 3 cut(s) 399, 445, 490
BcoDI GTCTC 2 cut(s) 123, 638
BfmI CTRYAG 2 cut(s) 105, 408
BfoI RGCGCY 2 cut(s) 375, 1140
BfuAI ACCTGC 2 cut(s) 190, 1177
BisI GCNGC 8 cut(s) 74, 77, 80, 168, 663, 822, 1146, 1171
BlsI GCNGC 8 cut(s) 75, 78, 81, 169, 664, 823, 1147, 1172
Bme1390I CCNGG 6 cut(s) 369, 399, 445, 465, 490, 591
BmeT110I CYCGRG 1 cut(s) 1203
BmiI GGNNCC 3 cut(s) 292, 373, 450
BmrFI CCNGG 6 cut(s) 369, 399, 445, 465, 490, 591
BmsI GCATC 4 cut(s) 674, 808, 873, 880
BpiI GAAGAC 1 cut(s) 643
BpmI CTGGAG 2 cut(s) 690, 1125
BpuEI CTTGAG 2 cut(s) 260, 1151
BpuMI CCSGG 3 cut(s) 399, 445, 490
Bsa29I ATCGAT 1 cut(s) 156
BsaAI YACGTR 1 cut(s) 10
BsaHI GRCGYC 1 cut(s) 372
BsaJI CCNNGG 1 cut(s) 444
BsaXI ACNNNNNCTCC 2 cut(s) 633, 663
Bsc4I CCNNNNNNNGG 4 cut(s) 368, 374, 413, 638
Bse118I RCCGGY 4 cut(s) 224, 374, 1078, 1166
Bse1I ACTGG 1 cut(s) 577
Bse21I CCTNAGG 1 cut(s) 843
Bse3DI GCAATG 1 cut(s) 1180
BseBI CCWGG 3 cut(s) 369, 465, 591
BseCI ATCGAT 1 cut(s) 156
BseDI CCNNGG 1 cut(s) 444
BseGI GGATG 2 cut(s) 823, 945
BseLI CCNNNNNNNGG 4 cut(s) 368, 374, 413, 638
BseMI GCAATG 1 cut(s) 1180
BseMII CTCAG 1 cut(s) 834
BseNI ACTGG 1 cut(s) 577
BseX3I CGGCCG 1 cut(s) 1168
BseXI GCAGC 6 cut(s) 85, 88, 91, 674, 833, 1132
BsgI GTGCAG 1 cut(s) 32
Bsh1285I CGRYCG 1 cut(s) 1171
BshFI GGCC 6 cut(s) 5, 693, 921, 1082, 1128, 1170
BshNI GGYRCC 1 cut(s) 371
BshVI ATCGAT 1 cut(s) 156
BsiEI CGRYCG 1 cut(s) 1171
BsiHKCI CYCGRG 1 cut(s) 1203
BsiSI CCGG 9 cut(s) 225, 375, 399, 444, 489, 735, 922, 1079, 1167
BslI CCNNNNNNNGG 4 cut(s) 368, 374, 413, 638
BsmAI GTCTC 2 cut(s) 123, 638
BsmBI CGTCTC 1 cut(s) 638
BsnI GGCC 6 cut(s) 5, 693, 921, 1082, 1128, 1170
BsoBI CYCGRG 1 cut(s) 1203
Bsp1286I GDGCHC 1 cut(s) 1161
Bsp143I GATC 4 cut(s) 157, 186, 448, 754
BspACI CCGC 8 cut(s) 35, 101, 167, 261, 349, 414, 967, 1171
BspANI GGCC 6 cut(s) 5, 693, 921, 1082, 1128, 1170
BspCNI CTCAG 1 cut(s) 835
BspDI ATCGAT 1 cut(s) 156
BspLI GGNNCC 3 cut(s) 292, 373, 450
BspMI ACCTGC 2 cut(s) 190, 1177
BspPI GGATC 3 cut(s) 152, 443, 456
BspQI GCTCTTC 1 cut(s) 798
BspT107I GGYRCC 1 cut(s) 371
BsrDI GCAATG 1 cut(s) 1180
BsrFI RCCGGY 4 cut(s) 224, 374, 1078, 1166
BsrI ACTGG 1 cut(s) 577
BssAI RCCGGY 4 cut(s) 224, 374, 1078, 1166
BssECI CCNNGG 1 cut(s) 444
BssMI GATC 4 cut(s) 157, 186, 448, 754
BssNI GRCGYC 1 cut(s) 372
Bst2UI CCWGG 3 cut(s) 369, 465, 591
Bst4CI ACNGT 3 cut(s) 506, 649, 1030
Bst6I CTCTTC 2 cut(s) 492, 798
BstACI GRCGYC 1 cut(s) 372
BstBAI YACGTR 1 cut(s) 10
BstC8I GCNNGC 3 cut(s) 1016, 1080, 1168
BstDEI CTNAG 3 cut(s) 633, 843, 1034
BstF5I GGATG 2 cut(s) 823, 945
BstH2I RGCGCY 2 cut(s) 375, 1140
BstHHI GCGC 2 cut(s) 374, 1139
BstKTI GATC 4 cut(s) 160, 189, 451, 757
BstMAI GTCTC 2 cut(s) 123, 638
BstMBI GATC 4 cut(s) 157, 186, 448, 754
BstMCI CGRYCG 1 cut(s) 1171
BstMWI GCNNNNNNNGC 5 cut(s) 79, 93, 671, 1134, 1145
BstNI CCWGG 3 cut(s) 369, 465, 591
BstNSI RCATGY 2 cut(s) 250, 1018
BstSCI CCNGG 6 cut(s) 367, 397, 443, 463, 488, 589
BstSFI CTRYAG 2 cut(s) 105, 408
BstV1I GCAGC 6 cut(s) 85, 88, 91, 674, 833, 1132
BstV2I GAAGAC 1 cut(s) 643
BstX2I RGATCY 1 cut(s) 448
BstXI CCANNNNNNTGG 2 cut(s) 54, 436
BstYI RGATCY 1 cut(s) 448
BstZI CGGCCG 1 cut(s) 1168
Bsu15I ATCGAT 1 cut(s) 156
Bsu36I CCTNAGG 1 cut(s) 843
BsuRI GGCC 6 cut(s) 5, 693, 921, 1082, 1128, 1170
BsuTUI ATCGAT 1 cut(s) 156
BtsCI GGATG 2 cut(s) 823, 945
BtsI GCAGTG 3 cut(s) 82, 513, 900
BtsIMutI CAGTG 5 cut(s) 82, 511, 513, 546, 900
BveI ACCTGC 2 cut(s) 190, 1177
Cac8I GCNNGC 3 cut(s) 1016, 1080, 1168
CaiI CAGNNNCTG 1 cut(s) 668
CfoI GCGC 2 cut(s) 374, 1139
Cfr10I RCCGGY 4 cut(s) 224, 374, 1078, 1166
ClaI ATCGAT 1 cut(s) 156
Csp6I GTAC 3 cut(s) 174, 502, 952
CviAII CATG 4 cut(s) 247, 352, 907, 1015
CviQI GTAC 3 cut(s) 174, 502, 952
DdeI CTNAG 3 cut(s) 633, 843, 1034
DinI GGCGCC 1 cut(s) 373
DpnI GATC 4 cut(s) 159, 188, 450, 756
DpnII GATC 4 cut(s) 157, 186, 448, 754
DraI TTTAAA 1 cut(s) 828
EaeI YGGCCR 4 cut(s) 691, 919, 1080, 1168
EagI CGGCCG 1 cut(s) 1168
Eam1104I CTCTTC 2 cut(s) 492, 798
EarI CTCTTC 2 cut(s) 492, 798
EciI GGCGGA 2 cut(s) 90, 429
EclXI CGGCCG 1 cut(s) 1168
Eco147I AGGCCT 1 cut(s) 1128
Eco24I GRGCYC 1 cut(s) 1161
Eco32I GATATC 1 cut(s) 945
Eco47III AGCGCT 1 cut(s) 1138
Eco52I CGGCCG 1 cut(s) 1168
Eco81I CCTNAGG 1 cut(s) 843
Eco88I CYCGRG 1 cut(s) 1203
EcoRI GAATTC 1 cut(s) 576
EcoRII CCWGG 3 cut(s) 367, 463, 589
EcoRV GATATC 1 cut(s) 945
EcoT38I GRGCYC 1 cut(s) 1161
EgeI GGCGCC 1 cut(s) 373
EheI GGCGCC 1 cut(s) 373
Esp3I CGTCTC 1 cut(s) 638
FaeI CATG 4 cut(s) 250, 355, 910, 1018
FatI CATG 4 cut(s) 246, 351, 906, 1014
FauI CCCGC 1 cut(s) 960
FbaI TGATCA 1 cut(s) 186
FblI GTMKAC 1 cut(s) 17
Fnu4HI GCNGC 8 cut(s) 74, 77, 80, 168, 663, 822, 1146, 1171
FokI GGATG 2 cut(s) 830, 952
FriOI GRGCYC 1 cut(s) 1161
Fsp4HI GCNGC 8 cut(s) 74, 77, 80, 168, 663, 822, 1146, 1171
GlaI GCGC 2 cut(s) 373, 1138
GluI GCNGC 8 cut(s) 74, 77, 80, 168, 663, 822, 1146, 1171
GsuI CTGGAG 2 cut(s) 690, 1125
HaeII RGCGCY 2 cut(s) 375, 1140
HaeIII GGCC 6 cut(s) 5, 693, 921, 1082, 1128, 1170
HapII CCGG 9 cut(s) 225, 375, 399, 444, 489, 735, 922, 1079, 1167
HhaI GCGC 2 cut(s) 374, 1139
Hin1I GRCGYC 1 cut(s) 372
Hin1II CATG 4 cut(s) 250, 355, 910, 1018
Hin6I GCGC 2 cut(s) 372, 1137
HinP1I GCGC 2 cut(s) 372, 1137
HindIII AAGCTT 1 cut(s) 278
HinfI GANTC 8 cut(s) 43, 58, 239, 345, 386, 440, 524, 538
HpaII CCGG 9 cut(s) 225, 375, 399, 444, 489, 735, 922, 1079, 1167
HphI GGTGA 2 cut(s) 250, 319
Hpy166II GTNNAC 3 cut(s) 18, 954, 1004
Hpy188I TCNGA 2 cut(s) 42, 244
Hpy188III TCNNGA 5 cut(s) 284, 669, 842, 1064, 1203
Hpy8I GTNNAC 3 cut(s) 18, 954, 1004
HpyAV CCTTC 6 cut(s) 281, 400, 631, 965, 1085, 1118
HpyCH4III ACNGT 3 cut(s) 506, 649, 1030
HpyCH4IV ACGT 1 cut(s) 9
HpyF10VI GCNNNNNNNGC 5 cut(s) 79, 93, 671, 1134, 1145
HpyF3I CTNAG 3 cut(s) 633, 843, 1034
HpySE526I ACGT 1 cut(s) 9
Hsp92I GRCGYC 1 cut(s) 372
Hsp92II CATG 4 cut(s) 250, 355, 910, 1018
HspAI GCGC 2 cut(s) 372, 1137
KasI GGCGCC 1 cut(s) 371
KroI GCCGGC 2 cut(s) 1078, 1166
KroNI GCCGGC 2 cut(s) 1080, 1168
Ksp22I TGATCA 1 cut(s) 186
Kzo9I GATC 4 cut(s) 157, 186, 448, 754
LguI GCTCTTC 1 cut(s) 798
LmnI GCTCC 2 cut(s) 671, 1144
Lsp1109I GCAGC 6 cut(s) 85, 88, 91, 674, 833, 1132
LweI GCATC 4 cut(s) 674, 808, 873, 880
MaeII ACGT 1 cut(s) 9
MaeIII GTNAC 3 cut(s) 220, 977, 1037
MalI GATC 4 cut(s) 159, 188, 450, 756
MboI GATC 4 cut(s) 157, 186, 448, 754
MboII GAAGA 6 cut(s) 322, 509, 643, 815, 1108, 1124
MflI RGATCY 1 cut(s) 448
MhlI GDGCHC 1 cut(s) 1161
MlsI TGGCCA 1 cut(s) 693
MluCI AATT 4 cut(s) 576, 695, 793, 982
MluNI TGGCCA 1 cut(s) 693
Mly113I GGCGCC 1 cut(s) 372
MlyI GAGTC 1 cut(s) 533
MmeI TCCRAC 1 cut(s) 706
MnlI CCTC 8 cut(s) 63, 108, 172, 326, 567, 613, 838, 903
Mox20I TGGCCA 1 cut(s) 693
MroNI GCCGGC 2 cut(s) 1078, 1166
MroXI GAANNNNTTC 1 cut(s) 1071
MscI TGGCCA 1 cut(s) 693
MseI TTAA 4 cut(s) 459, 534, 827, 986
MslI CAYNNNNRTG 2 cut(s) 549, 551
Msp20I TGGCCA 1 cut(s) 693
MspA1I CMGCKG 2 cut(s) 967, 1145
MspI CCGG 9 cut(s) 225, 375, 399, 444, 489, 735, 922, 1079, 1167
MspR9I CCNGG 6 cut(s) 369, 399, 445, 465, 490, 591
MvaI CCWGG 3 cut(s) 369, 465, 591
MwoI GCNNNNNNNGC 5 cut(s) 79, 93, 671, 1134, 1145
NaeI GCCGGC 2 cut(s) 1080, 1168
NarI GGCGCC 1 cut(s) 372
NciI CCSGG 3 cut(s) 399, 445, 490
NdeII GATC 4 cut(s) 157, 186, 448, 754
NgoMIV GCCGGC 2 cut(s) 1078, 1166
NlaIII CATG 4 cut(s) 250, 355, 910, 1018
NlaIV GGNNCC 3 cut(s) 292, 373, 450
NmuCI GTSAC 2 cut(s) 220, 977
NspI RCATGY 2 cut(s) 250, 1018
OliI CACNNNNGTG 1 cut(s) 551
PaeI GCATGC 1 cut(s) 1018
PaqCI CACCTGC 2 cut(s) 190, 1177
PceI AGGCCT 1 cut(s) 1128
PciSI GCTCTTC 1 cut(s) 798
PdiI GCCGGC 2 cut(s) 1080, 1168
PdmI GAANNNNTTC 1 cut(s) 1071
PfeI GAWTC 7 cut(s) 43, 58, 239, 345, 386, 440, 538
PkrI GCNGC 8 cut(s) 75, 78, 81, 169, 664, 823, 1147, 1172
PleI GAGTC 1 cut(s) 532
PluTI GGCGCC 1 cut(s) 375
PpsI GAGTC 1 cut(s) 532
Ppu21I YACGTR 1 cut(s) 10
PsiI TTATAA 1 cut(s) 798
Psp6I CCWGG 3 cut(s) 367, 463, 589
PspGI CCWGG 3 cut(s) 367, 463, 589
PspN4I GGNNCC 3 cut(s) 292, 373, 450
PstNI CAGNNNCTG 1 cut(s) 668
PsuI RGATCY 1 cut(s) 448
PvuII CAGCTG 1 cut(s) 1145
RsaI GTAC 3 cut(s) 175, 503, 953
RsaNI GTAC 3 cut(s) 174, 502, 952
RseI CAYNNNNRTG 2 cut(s) 549, 551
SapI GCTCTTC 1 cut(s) 798
SaqAI TTAA 4 cut(s) 459, 534, 827, 986
SatI GCNGC 8 cut(s) 74, 77, 80, 168, 663, 822, 1146, 1171
Sau3AI GATC 4 cut(s) 157, 186, 448, 754
SchI GAGTC 1 cut(s) 533
ScrFI CCNGG 6 cut(s) 369, 399, 445, 465, 490, 591
SduI GDGCHC 1 cut(s) 1161
SfaNI GCATC 4 cut(s) 674, 808, 873, 880
SfcI CTRYAG 2 cut(s) 105, 408
SfoI GGCGCC 1 cut(s) 373
SmiMI CAYNNNNRTG 2 cut(s) 549, 551
SmlI CTYRAG 2 cut(s) 275, 1130
SmoI CTYRAG 2 cut(s) 275, 1130
SphI GCATGC 1 cut(s) 1018
Sse9I AATT 4 cut(s) 576, 695, 793, 982
SseBI AGGCCT 1 cut(s) 1128
SsiI CCGC 8 cut(s) 35, 101, 167, 261, 349, 414, 967, 1171
SspDI GGCGCC 1 cut(s) 371
StuI AGGCCT 1 cut(s) 1128
StyD4I CCNGG 6 cut(s) 367, 397, 443, 463, 488, 589
TaaI ACNGT 3 cut(s) 506, 649, 1030
TaiI ACGT 1 cut(s) 12
TaqI TCGA 3 cut(s) 50, 156, 384
TasI AATT 4 cut(s) 576, 695, 793, 982
TatI WGTACW 3 cut(s) 173, 501, 951
TauI GCSGC 2 cut(s) 170, 1173
TfiI GAWTC 7 cut(s) 43, 58, 239, 345, 386, 440, 538
Tru1I TTAA 4 cut(s) 459, 534, 827, 986
Tru9I TTAA 4 cut(s) 459, 534, 827, 986
TscAI CASTG 5 cut(s) 89, 511, 520, 553, 900
TseFI GTSAC 2 cut(s) 220, 977
TseI GCWGC 6 cut(s) 73, 76, 79, 662, 821, 1145
Tsp45I GTSAC 2 cut(s) 220, 977
TspDTI ATGAA 1 cut(s) 463
TspRI CASTG 5 cut(s) 89, 511, 520, 553, 900
XapI RAATTY 1 cut(s) 576
XceI RCATGY 2 cut(s) 250, 1018
XcmI CCANNNNNNNNNTGG 1 cut(s) 301
XmiI GTMKAC 1 cut(s) 17
XmnI GAANNNNTTC 1 cut(s) 1071
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.