Rorug01G0459600

2-alkenal reductase (NADP( )-dependent)-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000196
Physical Location & Seq
Forward (+)
55326031 .. 55330513
4483 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0459600.1

Sequence Viewer

Length: 963 bp
ATGGATCCACAGAATCCAGCAGAAAAGCGATTCTCACTGCGCAATGACAAACAAAGTAGCATTGCAAAGAACAAGAGCAGCAAAGTCACACTTCAAGATATCCAAGTTGTCCATAATTTGATTGAAAGGTGTCTGCAGTTGTATATGAACAAAGATGAAGTCATCAATACCCTCTTAGATCGTGCTAGGATTGAGCCTGAGTTTACAAAATTGGTATGGCAGAAACTGGAAGAAGAAAATGCAGAATTTTTCAAGGCCTACCACCTAAGGCTGAAGCTCAAAGATCAAATTCATATGTATAACCGGTTGCTTGAGCAGCAATACCATCTGATGAAACAGCAAGAGCGTGCAGAGTTTAAGATAGCTCCTGTGCAGAATGGAATGCGTCACATGCCTGGTTATCCCGAAATCAATTCCATGGGCACCATCTCTAGTTGTCATGGAGTTAATGGAATACCTGCTCCAGGAAACTTCCACAGTATGCAACATAAGTGTGTGAAAGAAAGAAGTCCAGCTAATACAGTACCTTTCTTCCCCGCTGGTGATATGAAGTTAGATGGGGCTTCAAGTCCTGTAGCAGTGGCATTGAATGGACAATTTTCCTTCACTCCTGAAACATCAGGGCTAAATCTGGACACACCAGTGCTTGACTCTGCATTTACTTCTGATATAGCAAATCTAGAGAGGTTGCGTCTTGGAGCAGATGGTCAGAACACATGTCCTGAGGAGTCACTTCAATCATCGGATCAGATGACTTGGGATTTTGGCTTCTCTAATACTGCTGAATGGTCAAACTTTCAAGAGTTTGGACCAGCGGGGAACTATTCAGGCTCAGAGTTTCAACCTTCTCAGTTGGATATTAATCTTGATTCTGCAGAGCAAAATGACATGGTTGATGGGTTCCTTCTCGATGGTCCACCTGGTCAAGGCACTCAACCTGCAGAGGAAACTAAGCCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006950 GO:0006979 GO:0008150 GO:0016020 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N_2 ADH_zinc_N

Protein Analysis

320

Amino Acids

35.94

Weight (kDa)

5.1

Isoelectric Point (pI)

53.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
A_thal_3526 PF09713 37 - 88 1e-25 Plant protein 1589 of unknown function (A_thal_3526)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000196)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65560
fragaria_vesca FvH4_1g00330 FvH4_1g00342 FvH4_1g00343 FvH4_1g00344 FvH4_1g00345 FvH4_1g00345 FvH4_1g00346 FvH4_1g26520
malus_domestica MD02G1001800.v1.1 MD02G1001900.v1.1 MD02G1002100.v1.1 MD02G1002300.v1.1 MD02G1002400.v1.1 MD02G1002500.v1.1 MD02G1002600.v1.1 MD15G1145700.v1.1
prunus_persica Prupe.7G268600_v2.0.a1 Prupe.7G268700_v2.0.a1 Prupe.7G268800_v2.0.a1 Prupe.7G268800_v2.0.a1 Prupe.7G268900_v2.0.a1
pyrus_communis pycom02g00050 pycom02g00060 pycom02g00100 pycom02g00120 pycom02g00130 pycom15g13090 pycom15g13100
rosa_chinensis RchiOBHm_Chr1g0325321 RchiOBHm_Chr1g0325341 RchiOBHm_Chr1g0325351 RchiOBHm_Chr1g0325361 RchiOBHm_Chr1g0364881 RchiOBHm_Chr1g0364891 RchiOBHm_Chr2g0084701 RchiOBHm_Chr2g0084721 RchiOBHm_Chr2g0084731 RchiOBHm_Chr2g0084741 RchiOBHm_Chr2g0084751 RchiOBHm_Chr2g0084761 RchiOBHm_Chr2g0084771 RchiOBHm_Chr2g0085311 RchiOBHm_Chr2g0085321 RchiOBHm_Chr2g0085341 RchiOBHm_Chr2g0085351 RchiOBHm_Chr2g0117721 RchiOBHm_Chr2g0159631 RchiOBHm_Chr3g0484011 RchiOBHm_Chr5g0021141 RchiOBHm_Chr5g0061081 RchiOBHm_Chr6g0275731 RchiOBHm_Chr7g0238011
rosa_laevigata RLG00000013438 RLG00000015632 RLG00000015634 RLG00000015635 RLG00000015636 RLG00000015637 RLG00000015677 RLG00000015679 RLG00000030145 RLG00000030146 RLG00000035427
rosa_multiflora Rmu_co8334849.1_g000001 Rmu_sc0001366.1_g000035 Rmu_sc0002352.1_g000003 Rmu_sc0002352.1_g000004 Rmu_sc0002352.1_g000006 Rmu_sc0002352.1_g000007 Rmu_sc0002352.1_g000009 Rmu_sc0002586.1_g000008 Rmu_sc0004966.1_g000016 Rmu_sc0007122.1_g000001 Rmu_sc0008804.1_g000002 Rmu_sc0008883.1_g000001 Rmu_sc0010860.1_g000002 Rmu_sc0012119.1_g000001 Rmu_sc0012119.1_g000003 Rmu_sc0012119.1_g000006 Rmu_sc0012119.1_g000007 Rmu_sc0012119.1_g000009 Rmu_sc0020031.1_g000004 Rmu_sc0020800.1_g000005 Rmu_sc0020800.1_g000007 Rmu_sc0020800.1_g000008 Rmu_sc0020800.1_g000009 Rmu_sc0025597.1_g000001 Rmu_sc0029132.1_g000002
rosa_roxburghii Rroxscaffold_2G00155450 Rroxscaffold_2G00155460 Rroxscaffold_2G00155470 Rroxscaffold_2G00155830 Rroxscaffold_2G00155840 Rroxscaffold_2G00155850 Rroxscaffold_2G00155860 Rroxscaffold_2G00155870 Rroxscaffold_2G00155910 Rroxscaffold_3G00273850 Rroxscaffold_4G00324820 Rroxscaffold_7G00192930 Rroxscaffold_7G00192960
rosa_rugosa Rorug01G0051300 Rorug01G0051400 Rorug01G0455700 Rorug01G0455800 Rorug01G0455900 Rorug01G0459500 Rorug01G0459500 Rorug01G0459600 Rorug01G0459700 Rorug01G0459700 Rorug02G0335000
rosa_samantha Rh1AG066500 Rh1BG054700 Rh1BG054800 Rh1BG176600 Rh2BG004400 Rh2BG004500 Rh2BG004600 Rh2BG004700 Rh2BG008600 Rh2BG008700 Rh2CG004500 Rh2CG004700 Rh2CG004800 Rh2CG004900 Rh2CG005000 Rh2CG005100 Rh2CG005200 Rh2CG009300 Rh2CG009400 Rh2DG004200 Rh2DG004300 Rh2DG004400 Rh2DG004500 Rh2DG004600 Rh2DG009800 Rh5BG411800 Rh5CG167200 Rh5CG436800 Rh5CG578800 Rh6DG203800
rosa_wichuraiana Rw1G005560 Rw2G000320 Rw2G000350 Rw2G000360 Rw2G000370 Rw2G000750 Rw2G000760 Rw4G022130 Rw5G015810 Rw5G037620 Rw6G018120 Rw7G038530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 41
Acc36I ACCTGC 2 cut(s) 466, 946
AccB1I GGYRCC 1 cut(s) 422
AciI CCGC 2 cut(s) 537, 815
AclWI GGATC 2 cut(s) 12, 753
AcsI RAATTY 2 cut(s) 245, 288
AcuI CTGAAG 1 cut(s) 293
AfaI GTAC 1 cut(s) 525
AfiI CCNNNNNNNGG 2 cut(s) 464, 926
AflIII ACRYGT 1 cut(s) 716
AgeI ACCGGT 1 cut(s) 303
AgsI TTSAA 8 cut(s) 95, 125, 253, 567, 589, 737, 800, 842
AjnI CCWGG 3 cut(s) 394, 463, 919
AleI CACNNNNGTG 1 cut(s) 641
AluBI AGCT 3 cut(s) 277, 365, 515
AluI AGCT 3 cut(s) 277, 365, 515
AlwI GGATC 2 cut(s) 12, 753
AlwNI CAGNNNCTG 1 cut(s) 226
AoxI GGCC 1 cut(s) 255
ApeKI GCWGC 2 cut(s) 78, 316
ApoI RAATTY 2 cut(s) 245, 288
ArsI GACNNNNNNTTYG 2 cut(s) 144, 176
AseI ATTAAT 1 cut(s) 861
AsiGI ACCGGT 1 cut(s) 303
AspLEI GCGC 1 cut(s) 42
AspS9I GGNCC 2 cut(s) 809, 914
AsuHPI GGTGA 1 cut(s) 554
AvaII GGWCC 2 cut(s) 809, 914
AxyI CCTNAGG 2 cut(s) 266, 723
BaeGI GKGCMC 1 cut(s) 425
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 422
BbvI GCAGC 2 cut(s) 90, 328
BccI CCATC 6 cut(s) 333, 434, 551, 698, 890, 905
BciT130I CCWGG 3 cut(s) 396, 465, 921
BfaI CTAG 3 cut(s) 186, 432, 680
BfmI CTRYAG 4 cut(s) 134, 573, 873, 939
BfuAI ACCTGC 2 cut(s) 466, 946
BisI GCNGC 2 cut(s) 79, 317
BlsI GCNGC 2 cut(s) 80, 318
Bme1390I CCNGG 3 cut(s) 396, 465, 921
Bme18I GGWCC 2 cut(s) 809, 914
BmgT120I GGNCC 2 cut(s) 809, 914
BmiI GGNNCC 3 cut(s) 6, 424, 902
BmrFI CCNGG 3 cut(s) 396, 465, 921
BpmI CTGGAG 1 cut(s) 447
BpuEI CTTGAG 1 cut(s) 332
BsaBI GATNNNNATC 1 cut(s) 861
BsaJI CCNNGG 1 cut(s) 417
BsaWI WCCGGW 1 cut(s) 303
Bsc4I CCNNNNNNNGG 2 cut(s) 464, 926
Bse118I RCCGGY 1 cut(s) 303
Bse1I ACTGG 2 cut(s) 231, 641
Bse21I CCTNAGG 2 cut(s) 266, 723
Bse3DI GCAATG 2 cut(s) 49, 60
Bse8I GATNNNNATC 1 cut(s) 861
BseBI CCWGG 3 cut(s) 396, 465, 921
BseDI CCNNGG 1 cut(s) 417
BseJI GATNNNNATC 1 cut(s) 861
BseLI CCNNNNNNNGG 2 cut(s) 464, 926
BseMI GCAATG 2 cut(s) 49, 60
BseMII CTCAG 4 cut(s) 189, 714, 846, 863
BseNI ACTGG 2 cut(s) 231, 641
BseRI GAGGAG 1 cut(s) 740
BseSI GKGCMC 1 cut(s) 425
BseXI GCAGC 2 cut(s) 90, 328
BsgI GTGCAG 2 cut(s) 369, 392
BshFI GGCC 1 cut(s) 257
BshNI GGYRCC 1 cut(s) 422
BshTI ACCGGT 1 cut(s) 303
BsiSI CCGG 1 cut(s) 304
BslI CCNNNNNNNGG 2 cut(s) 464, 926
BsmI GAATGC 1 cut(s) 387
BsnI GGCC 1 cut(s) 257
Bsp1286I GDGCHC 1 cut(s) 425
Bsp143I GATC 4 cut(s) 4, 178, 283, 745
Bsp19I CCATGG 1 cut(s) 417
BspACI CCGC 2 cut(s) 537, 815
BspANI GGCC 1 cut(s) 257
BspCNI CTCAG 4 cut(s) 190, 715, 845, 862
BspLI GGNNCC 3 cut(s) 6, 424, 902
BspMAI CTGCAG 3 cut(s) 138, 877, 943
BspMI ACCTGC 2 cut(s) 466, 946
BspPI GGATC 2 cut(s) 12, 753
BspT107I GGYRCC 1 cut(s) 422
BsrDI GCAATG 2 cut(s) 49, 60
BsrFI RCCGGY 1 cut(s) 303
BsrI ACTGG 2 cut(s) 231, 641
BssAI RCCGGY 1 cut(s) 303
BssECI CCNNGG 1 cut(s) 417
BssMI GATC 4 cut(s) 4, 178, 283, 745
BssT1I CCWWGG 1 cut(s) 417
Bst2UI CCWGG 3 cut(s) 396, 465, 921
Bst4CI ACNGT 2 cut(s) 479, 523
BstC8I GCNNGC 1 cut(s) 348
BstDEI CTNAG 7 cut(s) 175, 198, 266, 723, 832, 849, 951
BstDSI CCRYGG 1 cut(s) 417
BstENI CCTNNNNNAGG 2 cut(s) 462, 924
BstHHI GCGC 1 cut(s) 42
BstKTI GATC 4 cut(s) 7, 181, 286, 748
BstMBI GATC 4 cut(s) 4, 178, 283, 745
BstMWI GCNNNNNNNGC 2 cut(s) 316, 391
BstNI CCWGG 3 cut(s) 396, 465, 921
BstNSI RCATGY 2 cut(s) 394, 720
BstSCI CCNGG 3 cut(s) 394, 463, 919
BstSFI CTRYAG 4 cut(s) 134, 573, 873, 939
BstSLI GKGCMC 1 cut(s) 425
BstV1I GCAGC 2 cut(s) 90, 328
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
Bsu36I CCTNAGG 2 cut(s) 266, 723
BsuRI GGCC 1 cut(s) 257
BtgI CCRYGG 1 cut(s) 417
BtsI GCAGTG 2 cut(s) 35, 585
BtsIMutI CAGTG 3 cut(s) 35, 585, 648
BveI ACCTGC 2 cut(s) 466, 946
Cac8I GCNNGC 1 cut(s) 348
CaiI CAGNNNCTG 1 cut(s) 226
CfoI GCGC 1 cut(s) 42
Cfr10I RCCGGY 1 cut(s) 303
Cfr13I GGNCC 2 cut(s) 809, 914
CseI GACGC 2 cut(s) 374, 680
CsiI ACCWGGT 1 cut(s) 919
Csp6I GTAC 1 cut(s) 524
CspAI ACCGGT 1 cut(s) 303
CviAII CATG 5 cut(s) 391, 418, 440, 717, 889
CviQI GTAC 1 cut(s) 524
DdeI CTNAG 7 cut(s) 175, 198, 266, 723, 832, 849, 951
DpnI GATC 4 cut(s) 6, 180, 285, 747
DpnII GATC 4 cut(s) 4, 178, 283, 745
Eco130I CCWWGG 1 cut(s) 417
Eco147I AGGCCT 1 cut(s) 257
Eco32I GATATC 1 cut(s) 100
Eco47I GGWCC 2 cut(s) 809, 914
Eco57I CTGAAG 1 cut(s) 293
Eco81I CCTNAGG 2 cut(s) 266, 723
EcoNI CCTNNNNNAGG 2 cut(s) 462, 924
EcoRII CCWGG 3 cut(s) 394, 463, 919
EcoRV GATATC 1 cut(s) 100
EcoT14I CCWWGG 1 cut(s) 417
ErhI CCWWGG 1 cut(s) 417
FaeI CATG 5 cut(s) 394, 421, 443, 720, 892
FalI AAGNNNNNCTT 2 cut(s) 75, 107
FatI CATG 5 cut(s) 390, 417, 439, 716, 888
FauI CCCGC 2 cut(s) 544, 808
FauNDI CATATG 1 cut(s) 294
Fnu4HI GCNGC 2 cut(s) 79, 317
Fsp4HI GCNGC 2 cut(s) 79, 317
FspBI CTAG 3 cut(s) 186, 432, 680
FspI TGCGCA 1 cut(s) 41
GlaI GCGC 1 cut(s) 41
GluI GCNGC 2 cut(s) 79, 317
GsuI CTGGAG 1 cut(s) 447
HaeIII GGCC 1 cut(s) 257
HapII CCGG 1 cut(s) 304
HgaI GACGC 2 cut(s) 374, 680
HhaI GCGC 1 cut(s) 42
Hin1II CATG 5 cut(s) 394, 421, 443, 720, 892
Hin6I GCGC 1 cut(s) 40
HinP1I GCGC 1 cut(s) 40
HinfI GANTC 5 cut(s) 13, 30, 650, 728, 869
HpaII CCGG 1 cut(s) 304
HphI GGTGA 1 cut(s) 554
Hpy166II GTNNAC 2 cut(s) 204, 917
Hpy188I TCNGA 6 cut(s) 330, 667, 711, 745, 750, 835
Hpy188III TCNNGA 9 cut(s) 95, 404, 611, 632, 680, 722, 800, 866, 908
Hpy8I GTNNAC 2 cut(s) 204, 917
HpyAV CCTTC 3 cut(s) 613, 855, 914
HpyCH4III ACNGT 2 cut(s) 479, 523
HpyCH4V TGCA 9 cut(s) 65, 136, 242, 350, 373, 484, 656, 875, 941
HpyF10VI GCNNNNNNNGC 2 cut(s) 316, 391
HpyF3I CTNAG 7 cut(s) 175, 198, 266, 723, 832, 849, 951
Hsp92II CATG 5 cut(s) 394, 421, 443, 720, 892
HspAI GCGC 1 cut(s) 40
Kzo9I GATC 4 cut(s) 4, 178, 283, 745
LmnI GCTCC 3 cut(s) 370, 466, 698
Lsp1109I GCAGC 2 cut(s) 90, 328
MabI ACCWGGT 1 cut(s) 919
MaeI CTAG 3 cut(s) 186, 432, 680
MaeIII GTNAC 3 cut(s) 85, 386, 729
MalI GATC 4 cut(s) 6, 180, 285, 747
MboI GATC 4 cut(s) 4, 178, 283, 745
MboII GAAGA 3 cut(s) 242, 245, 523
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 1 cut(s) 425
MluCI AATT 6 cut(s) 115, 209, 245, 288, 412, 596
MlyI GAGTC 2 cut(s) 644, 737
MmeI TCCRAC 1 cut(s) 834
MnlI CCTC 4 cut(s) 182, 678, 718, 937
MseI TTAA 3 cut(s) 357, 447, 861
MslI CAYNNNNRTG 2 cut(s) 492, 641
MspA1I CMGCKG 2 cut(s) 539, 815
MspI CCGG 1 cut(s) 304
MspR9I CCNGG 3 cut(s) 396, 465, 921
Mva1269I GAATGC 1 cut(s) 387
MvaI CCWGG 3 cut(s) 396, 465, 921
MwoI GCNNNNNNNGC 2 cut(s) 316, 391
NcoI CCATGG 1 cut(s) 417
NdeI CATATG 1 cut(s) 294
NdeII GATC 4 cut(s) 4, 178, 283, 745
NlaIII CATG 5 cut(s) 394, 421, 443, 720, 892
NlaIV GGNNCC 3 cut(s) 6, 424, 902
NmuCI GTSAC 3 cut(s) 85, 386, 729
NsbI TGCGCA 1 cut(s) 41
NspI RCATGY 2 cut(s) 394, 720
OliI CACNNNNGTG 1 cut(s) 641
PceI AGGCCT 1 cut(s) 257
PciI ACATGT 1 cut(s) 716
PctI GAATGC 1 cut(s) 387
PfeI GAWTC 3 cut(s) 13, 30, 869
PfoI TCCNGGA 1 cut(s) 463
PinAI ACCGGT 1 cut(s) 303
PkrI GCNGC 2 cut(s) 80, 318
PleI GAGTC 2 cut(s) 644, 736
PpsI GAGTC 2 cut(s) 644, 736
PscI ACATGT 1 cut(s) 716
PshBI ATTAAT 1 cut(s) 861
Psp6I CCWGG 3 cut(s) 394, 463, 919
PspGI CCWGG 3 cut(s) 394, 463, 919
PspN4I GGNNCC 3 cut(s) 6, 424, 902
PspPI GGNCC 2 cut(s) 809, 914
PstI CTGCAG 3 cut(s) 138, 877, 943
PstNI CAGNNNCTG 1 cut(s) 226
PsuI RGATCY 1 cut(s) 4
RsaI GTAC 1 cut(s) 525
RsaNI GTAC 1 cut(s) 524
RseI CAYNNNNRTG 2 cut(s) 492, 641
SaqAI TTAA 3 cut(s) 357, 447, 861
SatI GCNGC 2 cut(s) 79, 317
Sau3AI GATC 4 cut(s) 4, 178, 283, 745
Sau96I GGNCC 2 cut(s) 809, 914
SchI GAGTC 2 cut(s) 644, 737
ScrFI CCNGG 3 cut(s) 396, 465, 921
SduI GDGCHC 1 cut(s) 425
SexAI ACCWGGT 1 cut(s) 919
SfcI CTRYAG 4 cut(s) 134, 573, 873, 939
SinI GGWCC 2 cut(s) 809, 914
SmiMI CAYNNNNRTG 2 cut(s) 492, 641
SmlI CTYRAG 1 cut(s) 311
SmoI CTYRAG 1 cut(s) 311
Sse9I AATT 6 cut(s) 115, 209, 245, 288, 412, 596
SseBI AGGCCT 1 cut(s) 257
SsiI CCGC 2 cut(s) 537, 815
SspMI CTAG 3 cut(s) 186, 432, 680
StuI AGGCCT 1 cut(s) 257
StyD4I CCNGG 3 cut(s) 394, 463, 919
StyI CCWWGG 1 cut(s) 417
TaaI ACNGT 2 cut(s) 479, 523
TaqI TCGA 1 cut(s) 909
TasI AATT 6 cut(s) 115, 209, 245, 288, 412, 596
TfiI GAWTC 3 cut(s) 13, 30, 869
Tru1I TTAA 3 cut(s) 357, 447, 861
Tru9I TTAA 3 cut(s) 357, 447, 861
TscAI CASTG 3 cut(s) 42, 585, 648
TseFI GTSAC 3 cut(s) 85, 386, 729
TseI GCWGC 2 cut(s) 78, 316
Tsp45I GTSAC 3 cut(s) 85, 386, 729
TspDTI ATGAA 5 cut(s) 161, 171, 281, 347, 563
TspRI CASTG 3 cut(s) 42, 585, 648
VpaK11BI GGWCC 2 cut(s) 809, 914
VspI ATTAAT 1 cut(s) 861
XagI CCTNNNNNAGG 2 cut(s) 462, 924
XapI RAATTY 2 cut(s) 245, 288
XbaI TCTAGA 1 cut(s) 679
XceI RCATGY 2 cut(s) 394, 720
XspI CTAG 3 cut(s) 186, 432, 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.