FvH4_3g24890

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000352
Physical Location & Seq
Forward (+)
17961354 .. 17962078
725 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g24890.t1

Sequence Viewer

Length: 639 bp
ATGGCCAACTCTAAAGTTAGCCTGAAGCTACTCATCGATTCAAAGCGTCAGAAGGTTCTGTTTGCCGAAGCGGGTAAGGACTTTGTGGATTTTCTTTTCACCCTTCTCTCTTTGCCTGTTGGAACTGTCATTAGGCTACTGTCGAAGGATGCATGTGGCATGGTTGGTAGCTTTGGTAAACTGTATGAGAGTGTTGAAAGTCTAGACTGTACCTACATGCAACCTCAACTAAACAAGGATATCCTGCTCAAACCCAAGGCACCTGTTGCTGGACCCAATATCTTCGGTCTGTTGGCCAACGAACCAGCTGCTGGAGATAAGGTGTTCTACATGTGTTCAGATCCTAACAAGCAATGTCCAGAGTGCAAACAGTACATGTCTTCTCAGGCGACTTATGTTGGTCAACCACAGACCACAGTAGCATCTTCTAGCTTCAGTGCTGCAGGATATGTCAAAGGCGTTGTTACGTACATGATAATGGATGATTTGGAGGTGAAGCCTATGTCCACTATTTCGAGCATAACTCTGCTCAACAGGTTCAATGCTACTGATGTTGGTGCCCTCAAAGAGAAGGTTGTAGATCTCGGCATGAATGAGGTTAGTATTCAATTGCAGCATTCTCACTTCCTTTATTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

213

Amino Acids

23.17

Weight (kDa)

6.72

Isoelectric Point (pI)

45.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 3 - 206 7.7e-40 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000352)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g24890 FvH4_7g21280 FvH4_7g21300 FvH4_7g21300 FvH4_7g21310 FvH4_7g21330 FvH4_7g21340 FvH4_7g21600 FvH4_7g21610 FvH4_7g21630
malus_domestica MD07G1190400.v1.1 MD07G1190600.v1.1
prunus_persica Prupe.2G228600_v2.0.a1 Prupe.6G198200_v2.0.a1
pyrus_communis pycom07g18010
rosa_chinensis RchiOBHm_Chr1g0365711 RchiOBHm_Chr1g0365721 RchiOBHm_Chr1g0365731 RchiOBHm_Chr1g0365991 RchiOBHm_Chr5g0044511 RchiOBHm_Chr6g0247041 RchiOBHm_Chr6g0247081
rosa_laevigata RLG00000015223 RLG00000027405 RLG00000027406 RLG00000027407 RLG00000027408 RLG00000027409 RLG00000027411 RLG00000027413 RLG00000034277
rosa_multiflora Rmu_co8279495.1_g000001 Rmu_co8358963.1_g000001 Rmu_co8520123.1_g000003 Rmu_sc0002093.1_g000019 Rmu_sc0002093.1_g000024 Rmu_sc0002093.1_g000025 Rmu_sc0002093.1_g000029 Rmu_sc0002476.1_g000002 Rmu_sc0002476.1_g000021 Rmu_sc0003466.1_g000011 Rmu_sc0004288.1_g000006 Rmu_sc0004288.1_g000007 Rmu_sc0004311.1_g000022 Rmu_sc0004311.1_g000023 Rmu_sc0004311.1_g000026 Rmu_sc0004311.1_g000033 Rmu_sc0004577.1_g000008 Rmu_sc0004645.1_g000005 Rmu_sc0004645.1_g000006 Rmu_sc0006406.1_g000011 Rmu_sc0006406.1_g000012 Rmu_sc0009967.1_g000002 Rmu_sc0011598.1_g000003 Rmu_ssc0000151.1_g000015
rosa_roxburghii Rroxscaffold_1G00036500 Rroxscaffold_3G00276050 Rroxscaffold_4G00290950 Rroxscaffold_4G00290960 Rroxscaffold_4G00290970 Rroxscaffold_4G00290980 Rroxscaffold_4G00291000 Rroxscaffold_4G00291030 Rroxscaffold_4G00291060
rosa_rugosa Rorug01G0322900 Rorug01G0323000 Rorug01G0323100 Rorug01G0323200 Rorug01G0323300 Rorug01G0323400 Rorug01G0323500 Rorug01G0323600 Rorug05G0214700 Rorug05G0522100
rosa_samantha Rh1AG332000 Rh1AG332200 Rh1AG332300 Rh1AG334700 Rh1AG334800 Rh1AG335200 Rh1CG309900 Rh1CG310000 Rh1CG310100 Rh1DG325100 Rh1DG325300 Rh1DG325500 Rh5AG299000 Rh5BG306500 Rh5DG315800 Rh6AG037200 Rh6BG031900 Rh6BG032000 Rh6CG032300 Rh6CG032400 Rh6CG033200 Rh6DG030900
rosa_wichuraiana Rw0G001530 Rw1G019070 Rw1G029470 Rw1G029480 Rw1G029490 Rw1G029500 Rw1G029510 Rw1G029700 Rw5G027640 Rw5G027670 Rw6G003160 Rw6G003190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 259, 557
AccB7I CCANNNNNTGG 1 cut(s) 311
AciI CCGC 1 cut(s) 71
AclWI GGATC 1 cut(s) 335
AcoI YGGCCR 2 cut(s) 3, 294
AcuI CTGAAG 2 cut(s) 44, 418
AfaI GTAC 3 cut(s) 211, 374, 470
AfiI CCNNNNNNNGG 2 cut(s) 269, 311
AflIII ACRYGT 2 cut(s) 330, 375
AgsI TTSAA 4 cut(s) 42, 197, 541, 608
AluBI AGCT 4 cut(s) 28, 171, 308, 432
AluI AGCT 4 cut(s) 28, 171, 308, 432
AlwI GGATC 1 cut(s) 335
AlwNI CAGNNNCTG 1 cut(s) 311
AoxI GGCC 2 cut(s) 3, 294
ApeKI GCWGC 3 cut(s) 308, 440, 613
AspS9I GGNCC 1 cut(s) 272
AsuHPI GGTGA 2 cut(s) 91, 505
AvaII GGWCC 1 cut(s) 272
BaeGI GKGCMC 1 cut(s) 562
BalI TGGCCA 2 cut(s) 5, 296
BanI GGYRCC 2 cut(s) 259, 557
BbsI GAAGAC 1 cut(s) 372
BbvI GCAGC 3 cut(s) 295, 427, 625
BcgI CGANNNNNNTGC 2 cut(s) 290, 324
BfaI CTAG 2 cut(s) 203, 429
BfmI CTRYAG 1 cut(s) 441
BglII AGATCT 1 cut(s) 580
BisI GCNGC 3 cut(s) 309, 441, 614
BlsI GCNGC 3 cut(s) 310, 442, 615
Bme18I GGWCC 1 cut(s) 272
BmgT120I GGNCC 1 cut(s) 272
BmiI GGNNCC 3 cut(s) 261, 274, 559
BmsI GCATC 2 cut(s) 139, 431
BpiI GAAGAC 1 cut(s) 372
BplI GAGNNNNNCTC 2 cut(s) 508, 540
BpmI CTGGAG 1 cut(s) 333
Bsa29I ATCGAT 1 cut(s) 36
BsaAI YACGTR 1 cut(s) 468
BsaJI CCNNGG 1 cut(s) 255
BsaXI ACNNNNNCTCC 2 cut(s) 306, 336
Bsc4I CCNNNNNNNGG 2 cut(s) 269, 311
Bse3DI GCAATG 1 cut(s) 359
BseCI ATCGAT 1 cut(s) 36
BseDI CCNNGG 1 cut(s) 255
BseGI GGATG 2 cut(s) 154, 487
BseLI CCNNNNNNNGG 2 cut(s) 269, 311
BseMI GCAATG 1 cut(s) 359
BseMII CTCAG 1 cut(s) 398
BseSI GKGCMC 1 cut(s) 562
BseXI GCAGC 3 cut(s) 295, 427, 625
BshFI GGCC 2 cut(s) 5, 296
BshNI GGYRCC 2 cut(s) 259, 557
BshVI ATCGAT 1 cut(s) 36
BslI CCNNNNNNNGG 2 cut(s) 269, 311
BsmI GAATGC 1 cut(s) 616
BsnI GGCC 2 cut(s) 5, 296
Bsp1286I GDGCHC 1 cut(s) 562
Bsp143I GATC 2 cut(s) 340, 580
BspACI CCGC 1 cut(s) 71
BspANI GGCC 2 cut(s) 5, 296
BspCNI CTCAG 1 cut(s) 397
BspDI ATCGAT 1 cut(s) 36
BspLI GGNNCC 3 cut(s) 261, 274, 559
BspMAI CTGCAG 1 cut(s) 445
BspPI GGATC 1 cut(s) 335
BspT107I GGYRCC 2 cut(s) 259, 557
BsrDI GCAATG 1 cut(s) 359
BssECI CCNNGG 1 cut(s) 255
BssMI GATC 2 cut(s) 340, 580
BssT1I CCWWGG 1 cut(s) 255
Bst4CI ACNGT 6 cut(s) 127, 141, 183, 209, 372, 418
BstAPI GCANNNNNTGC 1 cut(s) 266
BstBAI YACGTR 1 cut(s) 468
BstDEI CTNAG 1 cut(s) 384
BstF5I GGATG 2 cut(s) 154, 487
BstKTI GATC 2 cut(s) 343, 583
BstMBI GATC 2 cut(s) 340, 580
BstMWI GCNNNNNNNGC 1 cut(s) 266
BstNSI RCATGY 4 cut(s) 156, 220, 334, 379
BstSFI CTRYAG 1 cut(s) 441
BstSLI GKGCMC 1 cut(s) 562
BstSNI TACGTA 1 cut(s) 468
BstV1I GCAGC 3 cut(s) 295, 427, 625
BstV2I GAAGAC 1 cut(s) 372
BstX2I RGATCY 2 cut(s) 340, 580
BstYI RGATCY 2 cut(s) 340, 580
Bsu15I ATCGAT 1 cut(s) 36
BsuRI GGCC 2 cut(s) 5, 296
BsuTUI ATCGAT 1 cut(s) 36
BtsCI GGATG 2 cut(s) 154, 487
BtsIMutI CAGTG 1 cut(s) 442
CaiI CAGNNNCTG 1 cut(s) 311
Cfr13I GGNCC 1 cut(s) 272
ClaI ATCGAT 1 cut(s) 36
CseI GACGC 1 cut(s) 35
Csp6I GTAC 3 cut(s) 210, 373, 469
CviAII CATG 7 cut(s) 153, 160, 217, 331, 376, 472, 589
CviJI RGCY 9 cut(s) 5, 21, 28, 136, 171, 296, 308, 432, 499
CviKI_1 RGCY 9 cut(s) 5, 21, 28, 136, 171, 296, 308, 432, 499
CviQI GTAC 3 cut(s) 210, 373, 469
DdeI CTNAG 1 cut(s) 384
DpnI GATC 2 cut(s) 342, 582
DpnII GATC 2 cut(s) 340, 580
EaeI YGGCCR 2 cut(s) 3, 294
Eco105I TACGTA 1 cut(s) 468
Eco130I CCWWGG 1 cut(s) 255
Eco32I GATATC 1 cut(s) 241
Eco47I GGWCC 1 cut(s) 272
Eco57I CTGAAG 2 cut(s) 44, 418
EcoRV GATATC 1 cut(s) 241
EcoT14I CCWWGG 1 cut(s) 255
EcoT22I ATGCAT 1 cut(s) 154
ErhI CCWWGG 1 cut(s) 255
FaeI CATG 7 cut(s) 156, 163, 220, 334, 379, 475, 592
FatI CATG 7 cut(s) 152, 159, 216, 330, 375, 471, 588
FauI CCCGC 1 cut(s) 64
Fnu4HI GCNGC 3 cut(s) 309, 441, 614
FokI GGATG 2 cut(s) 161, 494
Fsp4HI GCNGC 3 cut(s) 309, 441, 614
FspBI CTAG 2 cut(s) 203, 429
GluI GCNGC 3 cut(s) 309, 441, 614
GsuI CTGGAG 1 cut(s) 333
HaeIII GGCC 2 cut(s) 5, 296
HgaI GACGC 1 cut(s) 35
Hin1II CATG 7 cut(s) 156, 163, 220, 334, 379, 475, 592
HincII GTYRAC 1 cut(s) 404
HindII GTYRAC 1 cut(s) 404
HinfI GANTC 1 cut(s) 38
HphI GGTGA 2 cut(s) 91, 505
Hpy166II GTNNAC 3 cut(s) 179, 404, 507
Hpy188I TCNGA 2 cut(s) 51, 340
Hpy188III TCNNGA 2 cut(s) 203, 359
Hpy8I GTNNAC 3 cut(s) 179, 404, 507
HpyAV CCTTC 4 cut(s) 46, 113, 139, 565
HpyCH4III ACNGT 6 cut(s) 127, 141, 183, 209, 372, 418
HpyCH4IV ACGT 1 cut(s) 467
HpyCH4V TGCA 5 cut(s) 152, 220, 366, 443, 613
HpyF10VI GCNNNNNNNGC 1 cut(s) 266
HpyF3I CTNAG 1 cut(s) 384
HpySE526I ACGT 1 cut(s) 467
Hsp92II CATG 7 cut(s) 156, 163, 220, 334, 379, 475, 592
Kzo9I GATC 2 cut(s) 340, 580
Lsp1109I GCAGC 3 cut(s) 295, 427, 625
LweI GCATC 2 cut(s) 139, 431
MaeI CTAG 2 cut(s) 203, 429
MaeII ACGT 1 cut(s) 467
MaeIII GTNAC 1 cut(s) 463
MalI GATC 2 cut(s) 342, 582
MboI GATC 2 cut(s) 340, 580
MboII GAAGA 3 cut(s) 274, 372, 417
MfeI CAATTG 1 cut(s) 608
MflI RGATCY 2 cut(s) 340, 580
MhlI GDGCHC 1 cut(s) 562
MlsI TGGCCA 2 cut(s) 5, 296
MluCI AATT 1 cut(s) 608
MluNI TGGCCA 2 cut(s) 5, 296
MmeI TCCRAC 1 cut(s) 100
MnlI CCTC 4 cut(s) 234, 484, 572, 589
Mox20I TGGCCA 2 cut(s) 5, 296
Mph1103I ATGCAT 1 cut(s) 154
MscI TGGCCA 2 cut(s) 5, 296
MslI CAYNNNNRTG 1 cut(s) 476
Msp20I TGGCCA 2 cut(s) 5, 296
MspA1I CMGCKG 1 cut(s) 308
MunI CAATTG 1 cut(s) 608
Mva1269I GAATGC 1 cut(s) 616
MwoI GCNNNNNNNGC 1 cut(s) 266
NdeII GATC 2 cut(s) 340, 580
NlaIII CATG 7 cut(s) 156, 163, 220, 334, 379, 475, 592
NlaIV GGNNCC 3 cut(s) 261, 274, 559
NmeAIII GCCGAG 1 cut(s) 564
NsiI ATGCAT 1 cut(s) 154
NspI RCATGY 4 cut(s) 156, 220, 334, 379
PciI ACATGT 2 cut(s) 330, 375
PctI GAATGC 1 cut(s) 616
PfeI GAWTC 1 cut(s) 38
PflMI CCANNNNNTGG 1 cut(s) 311
PkrI GCNGC 3 cut(s) 310, 442, 615
Ppu21I YACGTR 1 cut(s) 468
PscI ACATGT 2 cut(s) 330, 375
PspN4I GGNNCC 3 cut(s) 261, 274, 559
PspPI GGNCC 1 cut(s) 272
PstI CTGCAG 1 cut(s) 445
PstNI CAGNNNCTG 1 cut(s) 311
PsuI RGATCY 2 cut(s) 340, 580
PvuII CAGCTG 1 cut(s) 308
RsaI GTAC 3 cut(s) 211, 374, 470
RsaNI GTAC 3 cut(s) 210, 373, 469
RseI CAYNNNNRTG 1 cut(s) 476
SatI GCNGC 3 cut(s) 309, 441, 614
Sau3AI GATC 2 cut(s) 340, 580
Sau96I GGNCC 1 cut(s) 272
SduI GDGCHC 1 cut(s) 562
SfaNI GCATC 2 cut(s) 139, 431
SfcI CTRYAG 1 cut(s) 441
SinI GGWCC 1 cut(s) 272
SmiMI CAYNNNNRTG 1 cut(s) 476
SnaBI TACGTA 1 cut(s) 468
Sse9I AATT 1 cut(s) 608
SsiI CCGC 1 cut(s) 71
SspMI CTAG 2 cut(s) 203, 429
StyI CCWWGG 1 cut(s) 255
TaaI ACNGT 6 cut(s) 127, 141, 183, 209, 372, 418
TaiI ACGT 1 cut(s) 470
TaqI TCGA 3 cut(s) 36, 143, 515
TaqII GACCGA 1 cut(s) 275
TasI AATT 1 cut(s) 608
TatI WGTACW 1 cut(s) 372
TfiI GAWTC 1 cut(s) 38
TscAI CASTG 1 cut(s) 442
TseI GCWGC 3 cut(s) 308, 440, 613
TspDTI ATGAA 1 cut(s) 605
TspRI CASTG 1 cut(s) 442
Van91I CCANNNNNTGG 1 cut(s) 311
VpaK11BI GGWCC 1 cut(s) 272
XbaI TCTAGA 1 cut(s) 202
XceI RCATGY 4 cut(s) 156, 220, 334, 379
XspI CTAG 2 cut(s) 203, 429
Zsp2I ATGCAT 1 cut(s) 154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.