Rorug05G0214700

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000352
Physical Location & Seq
Reverse (-)
22110296 .. 22115428
5133 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0214700.1

Sequence Viewer

Length: 642 bp
ATGGAACCACGAACTCTGCAACTGATGGAGCTACAAACCCAGCAACAAATCGAGGATGGAGGAAACAACCAAGCAGCCGGGAGTAGTGCCAACACAGATTTCTGGCGAAGCCGTGCCAGGTGGGTTCCTACTCCAGATCAAATAAGGATCCTCAAGGATCTTTACTACGACAAGGGAGTTAAGCGCCCAACTACAGAGCATATTCACGAGATCTGTCTCCAGCTGAACCAGTATGGACATGTTGAGGGCAAGAACATTTATTTTTGGTTCCAGAATGTCAGGGCTCGAGAGAAGCAGATGAAGAGGTGCAATCAGGCTGCTCAAGTGCCCATGGGAACTAGTTCTCCTGGTACTGCTGGTGGATCCATTGACATCAATTTTGGGTCCACTAGTTCTACTGATGATGGTAGATCCATTAATCTCAACTTTGGTTCCACCGATTCTACTGGAGGACAAACATCCCTGCAACAACGAGGAGGAGATCACCAGGAGATTGAAACCCTTCCATTGTTCCCCATGTCTGGTGAGGACATCTTTGGCAACATGAAGACTACTTCCGAGGGAGGTAGCGGTTATGGCGGTGGCTCTCGCATTTCCCTTGAGCTCAGTCTCAACTCCTCCGGAGCTGCTGATTTGGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

213

Amino Acids

23.25

Weight (kDa)

5.38

Isoelectric Point (pI)

55.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 38 - 99 9.1e-10 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000352)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g24890 FvH4_7g21280 FvH4_7g21300 FvH4_7g21300 FvH4_7g21310 FvH4_7g21330 FvH4_7g21340 FvH4_7g21600 FvH4_7g21610 FvH4_7g21630
malus_domestica MD07G1190400.v1.1 MD07G1190600.v1.1
prunus_persica Prupe.2G228600_v2.0.a1 Prupe.6G198200_v2.0.a1
pyrus_communis pycom07g18010
rosa_chinensis RchiOBHm_Chr1g0365711 RchiOBHm_Chr1g0365721 RchiOBHm_Chr1g0365731 RchiOBHm_Chr1g0365991 RchiOBHm_Chr5g0044511 RchiOBHm_Chr6g0247041 RchiOBHm_Chr6g0247081
rosa_laevigata RLG00000015223 RLG00000027405 RLG00000027406 RLG00000027407 RLG00000027408 RLG00000027409 RLG00000027411 RLG00000027413 RLG00000034277
rosa_multiflora Rmu_co8279495.1_g000001 Rmu_co8358963.1_g000001 Rmu_co8520123.1_g000003 Rmu_sc0002093.1_g000019 Rmu_sc0002093.1_g000024 Rmu_sc0002093.1_g000025 Rmu_sc0002093.1_g000029 Rmu_sc0002476.1_g000002 Rmu_sc0002476.1_g000021 Rmu_sc0003466.1_g000011 Rmu_sc0004288.1_g000006 Rmu_sc0004288.1_g000007 Rmu_sc0004311.1_g000022 Rmu_sc0004311.1_g000023 Rmu_sc0004311.1_g000026 Rmu_sc0004311.1_g000033 Rmu_sc0004577.1_g000008 Rmu_sc0004645.1_g000005 Rmu_sc0004645.1_g000006 Rmu_sc0006406.1_g000011 Rmu_sc0006406.1_g000012 Rmu_sc0009967.1_g000002 Rmu_sc0011598.1_g000003 Rmu_ssc0000151.1_g000015
rosa_roxburghii Rroxscaffold_1G00036500 Rroxscaffold_3G00276050 Rroxscaffold_4G00290950 Rroxscaffold_4G00290960 Rroxscaffold_4G00290970 Rroxscaffold_4G00290980 Rroxscaffold_4G00291000 Rroxscaffold_4G00291030 Rroxscaffold_4G00291060
rosa_rugosa Rorug01G0322900 Rorug01G0323000 Rorug01G0323100 Rorug01G0323200 Rorug01G0323300 Rorug01G0323400 Rorug01G0323500 Rorug01G0323600 Rorug05G0214700 Rorug05G0522100
rosa_samantha Rh1AG332000 Rh1AG332200 Rh1AG332300 Rh1AG334700 Rh1AG334800 Rh1AG335200 Rh1CG309900 Rh1CG310000 Rh1CG310100 Rh1DG325100 Rh1DG325300 Rh1DG325500 Rh5AG299000 Rh5BG306500 Rh5DG315800 Rh6AG037200 Rh6BG031900 Rh6BG032000 Rh6CG032300 Rh6CG032400 Rh6CG033200 Rh6DG030900
rosa_wichuraiana Rw0G001530 Rw1G019070 Rw1G029470 Rw1G029480 Rw1G029490 Rw1G029500 Rw1G029510 Rw1G029700 Rw5G027640 Rw5G027670 Rw6G003160 Rw6G003190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 620
AciI CCGC 2 cut(s) 570, 579
AclWI GGATC 6 cut(s) 142, 155, 165, 357, 370, 405
AfaI GTAC 1 cut(s) 352
AfiI CCNNNNNNNGG 1 cut(s) 521
AflIII ACRYGT 1 cut(s) 238
AgsI TTSAA 1 cut(s) 497
AhlI ACTAGT 2 cut(s) 338, 389
AjnI CCWGG 3 cut(s) 116, 346, 486
AjuI GAANNNNNNNTTGG 2 cut(s) 83, 115
AloI GAACNNNNNNTCC 2 cut(s) 328, 360
AluBI AGCT 4 cut(s) 31, 223, 604, 626
AluI AGCT 4 cut(s) 31, 223, 604, 626
Alw21I GWGCWC 1 cut(s) 606
Alw26I GTCTC 2 cut(s) 221, 614
AlwI GGATC 6 cut(s) 142, 155, 165, 357, 370, 405
Ama87I CYCGRG 1 cut(s) 285
Aor13HI TCCGGA 1 cut(s) 620
ApeKI GCWGC 3 cut(s) 74, 317, 626
ArsI GACNNNNNNTTYG 2 cut(s) 362, 394
AseI ATTAAT 1 cut(s) 417
Asp700I GAANNNNTTC 2 cut(s) 340, 501
AspLEI GCGC 1 cut(s) 186
AspS9I GGNCC 1 cut(s) 384
AsuC2I CCSGG 1 cut(s) 79
AsuHPI GGTGA 2 cut(s) 476, 536
AvaI CYCGRG 1 cut(s) 285
AvaII GGWCC 1 cut(s) 384
BaeGI GKGCMC 1 cut(s) 330
BamHI GGATCC 2 cut(s) 147, 362
BanII GRGCYC 2 cut(s) 286, 606
BauI CACGAG 1 cut(s) 206
BbsI GAAGAC 1 cut(s) 554
Bbv12I GWGCWC 1 cut(s) 606
BbvI GCAGC 3 cut(s) 86, 304, 613
BccI CCATC 3 cut(s) 19, 50, 398
BceAI ACGGC 1 cut(s) 96
BciT130I CCWGG 3 cut(s) 118, 348, 488
BcnI CCSGG 1 cut(s) 79
BcoDI GTCTC 2 cut(s) 221, 614
BcuI ACTAGT 2 cut(s) 338, 389
BfaI CTAG 2 cut(s) 339, 390
BfmI CTRYAG 1 cut(s) 192
BfoI RGCGCY 1 cut(s) 187
BglII AGATCT 1 cut(s) 210
BisI GCNGC 3 cut(s) 75, 318, 627
BlsI GCNGC 3 cut(s) 76, 319, 628
Bme1390I CCNGG 4 cut(s) 79, 118, 348, 488
Bme18I GGWCC 1 cut(s) 384
BmeT110I CYCGRG 1 cut(s) 285
BmgT120I GGNCC 1 cut(s) 384
BmiI GGNNCC 7 cut(s) 6, 126, 149, 269, 364, 385, 433
BmrFI CCNGG 4 cut(s) 79, 118, 348, 488
BpiI GAAGAC 1 cut(s) 554
BpmI CTGGAG 3 cut(s) 117, 203, 468
BpuEI CTTGAG 3 cut(s) 137, 306, 620
BpuMI CCSGG 1 cut(s) 79
BsaJI CCNNGG 2 cut(s) 330, 558
BsaWI WCCGGW 1 cut(s) 620
BsaXI ACNNNNNCTCC 2 cut(s) 328, 358
Bsc4I CCNNNNNNNGG 1 cut(s) 521
Bse1I ACTGG 2 cut(s) 229, 451
BseAI TCCGGA 1 cut(s) 620
BseBI CCWGG 3 cut(s) 118, 348, 488
BseDI CCNNGG 2 cut(s) 330, 558
BseGI GGATG 2 cut(s) 61, 458
BseLI CCNNNNNNNGG 1 cut(s) 521
BseMII CTCAG 1 cut(s) 619
BseNI ACTGG 2 cut(s) 229, 451
BseRI GAGGAG 3 cut(s) 489, 492, 607
BseSI GKGCMC 1 cut(s) 330
BseXI GCAGC 3 cut(s) 86, 304, 613
BseYI CCCAGC 1 cut(s) 39
BsiHKAI GWGCWC 1 cut(s) 606
BsiHKCI CYCGRG 1 cut(s) 285
BsiSI CCGG 2 cut(s) 78, 621
BslI CCNNNNNNNGG 1 cut(s) 521
BsmAI GTCTC 2 cut(s) 221, 614
BsoBI CYCGRG 1 cut(s) 285
Bsp1286I GDGCHC 3 cut(s) 286, 330, 606
Bsp13I TCCGGA 1 cut(s) 620
Bsp143I GATC 7 cut(s) 136, 147, 157, 210, 362, 410, 481
Bsp19I CCATGG 1 cut(s) 330
BspACI CCGC 2 cut(s) 570, 579
BspCNI CTCAG 1 cut(s) 618
BspEI TCCGGA 1 cut(s) 620
BspLI GGNNCC 7 cut(s) 6, 126, 149, 269, 364, 385, 433
BspPI GGATC 6 cut(s) 142, 155, 165, 357, 370, 405
BsrI ACTGG 2 cut(s) 229, 451
BssECI CCNNGG 2 cut(s) 330, 558
BssMI GATC 7 cut(s) 136, 147, 157, 210, 362, 410, 481
BssSI CACGAG 1 cut(s) 206
BssT1I CCWWGG 1 cut(s) 330
Bst2BI CACGAG 1 cut(s) 206
Bst2UI CCWGG 3 cut(s) 118, 348, 488
Bst6I CTCTTC 1 cut(s) 296
BstDEI CTNAG 1 cut(s) 605
BstDSI CCRYGG 1 cut(s) 330
BstF5I GGATG 2 cut(s) 61, 458
BstH2I RGCGCY 1 cut(s) 187
BstHHI GCGC 1 cut(s) 186
BstKTI GATC 7 cut(s) 139, 150, 160, 213, 365, 413, 484
BstMAI GTCTC 2 cut(s) 221, 614
BstMBI GATC 7 cut(s) 136, 147, 157, 210, 362, 410, 481
BstMWI GCNNNNNNNGC 2 cut(s) 576, 635
BstNI CCWGG 3 cut(s) 118, 348, 488
BstNSI RCATGY 1 cut(s) 242
BstSCI CCNGG 4 cut(s) 77, 116, 346, 486
BstSFI CTRYAG 1 cut(s) 192
BstSLI GKGCMC 1 cut(s) 330
BstV1I GCAGC 3 cut(s) 86, 304, 613
BstV2I GAAGAC 1 cut(s) 554
BstX2I RGATCY 5 cut(s) 147, 157, 210, 362, 410
BstYI RGATCY 5 cut(s) 147, 157, 210, 362, 410
BtgI CCRYGG 1 cut(s) 330
BtsCI GGATG 2 cut(s) 61, 458
CfoI GCGC 1 cut(s) 186
Cfr13I GGNCC 1 cut(s) 384
Csp6I GTAC 1 cut(s) 351
CviAII CATG 4 cut(s) 239, 331, 517, 544
CviJI RGCY 9 cut(s) 31, 77, 111, 223, 284, 317, 585, 604, 626
CviKI_1 RGCY 9 cut(s) 31, 77, 111, 223, 284, 317, 585, 604, 626
CviQI GTAC 1 cut(s) 351
DdeI CTNAG 1 cut(s) 605
DpnI GATC 7 cut(s) 138, 149, 159, 212, 364, 412, 483
DpnII GATC 7 cut(s) 136, 147, 157, 210, 362, 410, 481
Eam1104I CTCTTC 1 cut(s) 296
EarI CTCTTC 1 cut(s) 296
Ecl136II GAGCTC 1 cut(s) 604
Eco130I CCWWGG 1 cut(s) 330
Eco24I GRGCYC 2 cut(s) 286, 606
Eco47I GGWCC 1 cut(s) 384
Eco53kI GAGCTC 1 cut(s) 604
Eco88I CYCGRG 1 cut(s) 285
EcoICRI GAGCTC 1 cut(s) 604
EcoRII CCWGG 3 cut(s) 116, 346, 486
EcoT14I CCWWGG 1 cut(s) 330
EcoT38I GRGCYC 2 cut(s) 286, 606
ErhI CCWWGG 1 cut(s) 330
FaeI CATG 4 cut(s) 242, 334, 520, 547
FaiI YATR 8 cut(s) 201, 234, 240, 332, 518, 545, 576, 640
FatI CATG 4 cut(s) 238, 330, 516, 543
Fnu4HI GCNGC 3 cut(s) 75, 318, 627
FokI GGATG 2 cut(s) 68, 445
FriOI GRGCYC 2 cut(s) 286, 606
Fsp4HI GCNGC 3 cut(s) 75, 318, 627
FspBI CTAG 2 cut(s) 339, 390
GlaI GCGC 1 cut(s) 185
GluI GCNGC 3 cut(s) 75, 318, 627
GsaI CCCAGC 1 cut(s) 43
GsuI CTGGAG 3 cut(s) 117, 203, 468
HaeII RGCGCY 1 cut(s) 187
HapII CCGG 2 cut(s) 78, 621
HhaI GCGC 1 cut(s) 186
Hin1II CATG 4 cut(s) 242, 334, 520, 547
Hin6I GCGC 1 cut(s) 184
HinP1I GCGC 1 cut(s) 184
HinfI GANTC 1 cut(s) 440
HpaII CCGG 2 cut(s) 78, 621
HphI GGTGA 2 cut(s) 476, 536
Hpy166II GTNNAC 1 cut(s) 387
Hpy188I TCNGA 1 cut(s) 559
Hpy188III TCNNGA 5 cut(s) 134, 206, 271, 287, 621
Hpy8I GTNNAC 1 cut(s) 387
HpyAV CCTTC 1 cut(s) 512
HpyCH4V TGCA 3 cut(s) 19, 309, 466
HpyF10VI GCNNNNNNNGC 2 cut(s) 576, 635
HpyF3I CTNAG 1 cut(s) 605
Hsp92II CATG 4 cut(s) 242, 334, 520, 547
HspAI GCGC 1 cut(s) 184
Kpn2I TCCGGA 1 cut(s) 620
Kzo9I GATC 7 cut(s) 136, 147, 157, 210, 362, 410, 481
LmnI GCTCC 2 cut(s) 28, 623
Lsp1109I GCAGC 3 cut(s) 86, 304, 613
MaeI CTAG 2 cut(s) 339, 390
MalI GATC 7 cut(s) 138, 149, 159, 212, 364, 412, 483
MboI GATC 7 cut(s) 136, 147, 157, 210, 362, 410, 481
MboII GAAGA 2 cut(s) 313, 559
MflI RGATCY 5 cut(s) 147, 157, 210, 362, 410
MhlI GDGCHC 3 cut(s) 286, 330, 606
MluCI AATT 1 cut(s) 376
MroI TCCGGA 1 cut(s) 620
MroXI GAANNNNTTC 2 cut(s) 340, 501
MseI TTAA 2 cut(s) 180, 417
MspA1I CMGCKG 1 cut(s) 223
MspI CCGG 2 cut(s) 78, 621
MspR9I CCNGG 4 cut(s) 79, 118, 348, 488
MvaI CCWGG 3 cut(s) 118, 348, 488
MwoI GCNNNNNNNGC 2 cut(s) 576, 635
NciI CCSGG 1 cut(s) 79
NcoI CCATGG 1 cut(s) 330
NdeII GATC 7 cut(s) 136, 147, 157, 210, 362, 410, 481
NlaIII CATG 4 cut(s) 242, 334, 520, 547
NlaIV GGNNCC 7 cut(s) 6, 126, 149, 269, 364, 385, 433
NspI RCATGY 1 cut(s) 242
PaeR7I CTCGAG 1 cut(s) 285
PciI ACATGT 1 cut(s) 238
PdmI GAANNNNTTC 2 cut(s) 340, 501
PfeI GAWTC 1 cut(s) 440
PkrI GCNGC 3 cut(s) 76, 319, 628
PscI ACATGT 1 cut(s) 238
PshBI ATTAAT 1 cut(s) 417
Psp124BI GAGCTC 1 cut(s) 606
Psp6I CCWGG 3 cut(s) 116, 346, 486
PspFI CCCAGC 1 cut(s) 39
PspGI CCWGG 3 cut(s) 116, 346, 486
PspN4I GGNNCC 7 cut(s) 6, 126, 149, 269, 364, 385, 433
PspPI GGNCC 1 cut(s) 384
PsuI RGATCY 5 cut(s) 147, 157, 210, 362, 410
PvuII CAGCTG 1 cut(s) 223
RsaI GTAC 1 cut(s) 352
RsaNI GTAC 1 cut(s) 351
SacI GAGCTC 1 cut(s) 606
SaqAI TTAA 2 cut(s) 180, 417
SatI GCNGC 3 cut(s) 75, 318, 627
Sau3AI GATC 7 cut(s) 136, 147, 157, 210, 362, 410, 481
Sau96I GGNCC 1 cut(s) 384
ScrFI CCNGG 4 cut(s) 79, 118, 348, 488
SduI GDGCHC 3 cut(s) 286, 330, 606
SetI ASST 7 cut(s) 33, 122, 225, 308, 568, 606, 628
SfcI CTRYAG 1 cut(s) 192
Sfr274I CTCGAG 1 cut(s) 285
SinI GGWCC 1 cut(s) 384
SlaI CTCGAG 1 cut(s) 285
SmlI CTYRAG 4 cut(s) 152, 285, 321, 599
SmoI CTYRAG 4 cut(s) 152, 285, 321, 599
SpeI ACTAGT 2 cut(s) 338, 389
Sse9I AATT 1 cut(s) 376
SsiI CCGC 2 cut(s) 570, 579
SspMI CTAG 2 cut(s) 339, 390
SstI GAGCTC 1 cut(s) 606
StyD4I CCNGG 4 cut(s) 77, 116, 346, 486
StyI CCWWGG 1 cut(s) 330
TaqI TCGA 2 cut(s) 51, 286
TasI AATT 1 cut(s) 376
TfiI GAWTC 1 cut(s) 440
Tru1I TTAA 2 cut(s) 180, 417
Tru9I TTAA 2 cut(s) 180, 417
TseI GCWGC 3 cut(s) 74, 317, 626
TspDTI ATGAA 2 cut(s) 314, 560
VpaK11BI GGWCC 1 cut(s) 384
VspI ATTAAT 1 cut(s) 417
XceI RCATGY 1 cut(s) 242
XhoI CTCGAG 1 cut(s) 285
XmnI GAANNNNTTC 2 cut(s) 340, 501
XspI CTAG 2 cut(s) 339, 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.