Rroxscaffold_4G00290970

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000352
Physical Location & Seq
Forward (+)
11392774 .. 11394374
1601 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00290970.1

Sequence Viewer

Length: 762 bp
ATGGCAACTATTAGCTTGAAGCTACTCGTTGACACAAAGCAGAAAAAAGTTCTGTTCGCCGAAGCAGGTAAGGAATTTGTTGACTTCCTCTTCACCCTTCTTTCTTTTCCCGCCGGTACCATCATCAGGCTCCTCTCAAAGGACAACATGGTTGGAAGCCTAGGTAAACTGTACGAAAGCGTTGAAAATTTTAGTGATACATATATGCAACCCAATCTGAACAAAGACATCCTCCTGAAACCTAAAGAACCTGGTACTGTGCCTAATAACCTAGGGTTGTTAACCAATGTCAAAAAAACTGAAAGAAAGTCGACTTACATTTGTTCCAATACGGGAAGCGTAGTTTGGCATGGAAGCTACAGTGCCTATAGCGATATCCACCACCCGTATTGTTCTGATGATCCTAATGCCACATGTCCAAAGTGCAATCACAAAATGAGTATTTCTACGACTTATGTTGCTCCACCGACTGCTCCGGCTGAAGAATCTTCCGCCTCCTCTAAAAGGATAGGGTTTGTCAAACCGGTCGTTACATACATGATAATGGATGACTTGGAAGTGAAGCCTATGTCTACCATCTCAACTATAGCTCTGCTCAACAGCTTTAATGTTAGAGATTTTGGTGCCCTTGAAGAGAAGGTTGTTAATCTAGGCATGGATGAGGGTGTGAAATTGCTCAAGGAATCATTGCAATCAAAGTCAGTTCTTACTAATGTGTTTCTTGGAGGAGTGAAGCATGAAGATCATAGCTCTGCAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

253

Amino Acids

27.78

Weight (kDa)

7.0

Isoelectric Point (pI)

32.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 3 - 245 9.5e-48 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000352)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g24890 FvH4_7g21280 FvH4_7g21300 FvH4_7g21300 FvH4_7g21310 FvH4_7g21330 FvH4_7g21340 FvH4_7g21600 FvH4_7g21610 FvH4_7g21630
malus_domestica MD07G1190400.v1.1 MD07G1190600.v1.1
prunus_persica Prupe.2G228600_v2.0.a1 Prupe.6G198200_v2.0.a1
pyrus_communis pycom07g18010
rosa_chinensis RchiOBHm_Chr1g0365711 RchiOBHm_Chr1g0365721 RchiOBHm_Chr1g0365731 RchiOBHm_Chr1g0365991 RchiOBHm_Chr5g0044511 RchiOBHm_Chr6g0247041 RchiOBHm_Chr6g0247081
rosa_laevigata RLG00000015223 RLG00000027405 RLG00000027406 RLG00000027407 RLG00000027408 RLG00000027409 RLG00000027411 RLG00000027413 RLG00000034277
rosa_multiflora Rmu_co8279495.1_g000001 Rmu_co8358963.1_g000001 Rmu_co8520123.1_g000003 Rmu_sc0002093.1_g000019 Rmu_sc0002093.1_g000024 Rmu_sc0002093.1_g000025 Rmu_sc0002093.1_g000029 Rmu_sc0002476.1_g000002 Rmu_sc0002476.1_g000021 Rmu_sc0003466.1_g000011 Rmu_sc0004288.1_g000006 Rmu_sc0004288.1_g000007 Rmu_sc0004311.1_g000022 Rmu_sc0004311.1_g000023 Rmu_sc0004311.1_g000026 Rmu_sc0004311.1_g000033 Rmu_sc0004577.1_g000008 Rmu_sc0004645.1_g000005 Rmu_sc0004645.1_g000006 Rmu_sc0006406.1_g000011 Rmu_sc0006406.1_g000012 Rmu_sc0009967.1_g000002 Rmu_sc0011598.1_g000003 Rmu_ssc0000151.1_g000015
rosa_roxburghii Rroxscaffold_1G00036500 Rroxscaffold_3G00276050 Rroxscaffold_4G00290950 Rroxscaffold_4G00290960 Rroxscaffold_4G00290970 Rroxscaffold_4G00290980 Rroxscaffold_4G00291000 Rroxscaffold_4G00291030 Rroxscaffold_4G00291060
rosa_rugosa Rorug01G0322900 Rorug01G0323000 Rorug01G0323100 Rorug01G0323200 Rorug01G0323300 Rorug01G0323400 Rorug01G0323500 Rorug01G0323600 Rorug05G0214700 Rorug05G0522100
rosa_samantha Rh1AG332000 Rh1AG332200 Rh1AG332300 Rh1AG334700 Rh1AG334800 Rh1AG335200 Rh1CG309900 Rh1CG310000 Rh1CG310100 Rh1DG325100 Rh1DG325300 Rh1DG325500 Rh5AG299000 Rh5BG306500 Rh5DG315800 Rh6AG037200 Rh6BG031900 Rh6BG032000 Rh6CG032300 Rh6CG032400 Rh6CG033200 Rh6DG030900
rosa_wichuraiana Rw0G001530 Rw1G019070 Rw1G029470 Rw1G029480 Rw1G029490 Rw1G029500 Rw1G029510 Rw1G029700 Rw5G027640 Rw5G027670 Rw6G003160 Rw6G003190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 56
Acc65I GGTACC 1 cut(s) 116
AccB1I GGYRCC 2 cut(s) 116, 623
AccI GTMKAC 2 cut(s) 311, 572
AciI CCGC 2 cut(s) 111, 492
AclWI GGATC 1 cut(s) 395
AcsI RAATTY 2 cut(s) 74, 187
AcuI CTGAAG 1 cut(s) 501
AfaI GTAC 3 cut(s) 118, 173, 256
AfiI CCNNNNNNNGG 3 cut(s) 126, 139, 504
AflIII ACRYGT 1 cut(s) 413
AgeI ACCGGT 1 cut(s) 523
AgsI TTSAA 3 cut(s) 19, 185, 632
AjnI CCWGG 1 cut(s) 250
AjuI GAANNNNNNNTTGG 2 cut(s) 328, 360
AluBI AGCT 6 cut(s) 15, 22, 357, 590, 603, 750
AluI AGCT 6 cut(s) 15, 22, 357, 590, 603, 750
AlwI GGATC 1 cut(s) 395
ApoI RAATTY 2 cut(s) 74, 187
AsiGI ACCGGT 1 cut(s) 523
Asp718I GGTACC 1 cut(s) 116
AspA2I CCTAGG 2 cut(s) 160, 271
AsuHPI GGTGA 1 cut(s) 85
AvrII CCTAGG 2 cut(s) 160, 271
BaeGI GKGCMC 1 cut(s) 628
BanI GGYRCC 2 cut(s) 116, 623
BccI CCATC 2 cut(s) 128, 584
BciT130I CCWGG 1 cut(s) 252
BfaI CTAG 3 cut(s) 161, 272, 650
BfmI CTRYAG 3 cut(s) 358, 367, 585
BfuAI ACCTGC 1 cut(s) 56
BlnI CCTAGG 2 cut(s) 160, 271
Bme1390I CCNGG 1 cut(s) 252
BmiI GGNNCC 3 cut(s) 118, 131, 625
BmrFI CCNGG 1 cut(s) 252
BpuEI CTTGAG 1 cut(s) 662
BsaJI CCNNGG 2 cut(s) 160, 271
BsaWI WCCGGW 1 cut(s) 523
Bsc4I CCNNNNNNNGG 3 cut(s) 126, 139, 504
Bse118I RCCGGY 2 cut(s) 113, 523
Bse3DI GCAATG 1 cut(s) 686
BseBI CCWGG 1 cut(s) 252
BseDI CCNNGG 2 cut(s) 160, 271
BseGI GGATG 3 cut(s) 228, 553, 664
BseLI CCNNNNNNNGG 3 cut(s) 126, 139, 504
BseMI GCAATG 1 cut(s) 686
BseRI GAGGAG 3 cut(s) 122, 487, 741
BseSI GKGCMC 1 cut(s) 628
Bsh1285I CGRYCG 1 cut(s) 528
BshNI GGYRCC 2 cut(s) 116, 623
BshTI ACCGGT 1 cut(s) 523
BsiEI CGRYCG 1 cut(s) 528
BsiSI CCGG 3 cut(s) 114, 476, 524
BslI CCNNNNNNNGG 3 cut(s) 126, 139, 504
Bsp1286I GDGCHC 1 cut(s) 628
Bsp143I GATC 2 cut(s) 400, 742
BspACI CCGC 2 cut(s) 111, 492
BspLI GGNNCC 3 cut(s) 118, 131, 625
BspMI ACCTGC 1 cut(s) 56
BspPI GGATC 1 cut(s) 395
BspT107I GGYRCC 2 cut(s) 116, 623
BsrDI GCAATG 1 cut(s) 686
BsrFI RCCGGY 2 cut(s) 113, 523
BssAI RCCGGY 2 cut(s) 113, 523
BssECI CCNNGG 2 cut(s) 160, 271
BssMI GATC 2 cut(s) 400, 742
BssT1I CCWWGG 2 cut(s) 160, 271
Bst2UI CCWGG 1 cut(s) 252
Bst4CI ACNGT 3 cut(s) 171, 259, 362
Bst6I CTCTTC 2 cut(s) 95, 627
BstENI CCTNNNNNAGG 2 cut(s) 137, 502
BstF5I GGATG 3 cut(s) 228, 553, 664
BstKTI GATC 2 cut(s) 403, 745
BstMBI GATC 2 cut(s) 400, 742
BstMCI CGRYCG 1 cut(s) 528
BstNI CCWGG 1 cut(s) 252
BstNSI RCATGY 1 cut(s) 417
BstSCI CCNGG 1 cut(s) 250
BstSFI CTRYAG 3 cut(s) 358, 367, 585
BstSLI GKGCMC 1 cut(s) 628
BtsCI GGATG 3 cut(s) 228, 553, 664
BtsIMutI CAGTG 1 cut(s) 367
BveI ACCTGC 1 cut(s) 56
Cfr10I RCCGGY 2 cut(s) 113, 523
CsiI ACCWGGT 1 cut(s) 250
Csp6I GTAC 3 cut(s) 117, 172, 255
CspAI ACCGGT 1 cut(s) 523
CspCI CAANNNNNGTGG 2 cut(s) 371, 406
CviAII CATG 6 cut(s) 148, 350, 414, 538, 655, 737
CviQI GTAC 3 cut(s) 117, 172, 255
DpnI GATC 2 cut(s) 402, 744
DpnII GATC 2 cut(s) 400, 742
Eam1104I CTCTTC 2 cut(s) 95, 627
EarI CTCTTC 2 cut(s) 95, 627
EciI GGCGGA 1 cut(s) 481
Eco130I CCWWGG 2 cut(s) 160, 271
Eco32I GATATC 1 cut(s) 376
Eco57I CTGAAG 1 cut(s) 501
EcoNI CCTNNNNNAGG 2 cut(s) 137, 502
EcoRII CCWGG 1 cut(s) 250
EcoRV GATATC 1 cut(s) 376
EcoT14I CCWWGG 2 cut(s) 160, 271
ErhI CCWWGG 2 cut(s) 160, 271
FaeI CATG 6 cut(s) 151, 353, 417, 541, 658, 740
FatI CATG 6 cut(s) 147, 349, 413, 537, 654, 736
FauI CCCGC 1 cut(s) 118
FblI GTMKAC 2 cut(s) 311, 572
FokI GGATG 3 cut(s) 215, 560, 671
FspBI CTAG 3 cut(s) 161, 272, 650
HapII CCGG 3 cut(s) 114, 476, 524
Hin1II CATG 6 cut(s) 151, 353, 417, 541, 658, 740
HincII GTYRAC 4 cut(s) 31, 82, 282, 312
HindII GTYRAC 4 cut(s) 31, 82, 282, 312
HinfI GANTC 2 cut(s) 485, 683
HpaI GTTAAC 1 cut(s) 282
HpaII CCGG 3 cut(s) 114, 476, 524
HphI GGTGA 1 cut(s) 85
Hpy166II GTNNAC 6 cut(s) 31, 82, 167, 282, 312, 573
Hpy188I TCNGA 2 cut(s) 219, 397
Hpy188III TCNNGA 1 cut(s) 235
Hpy8I GTNNAC 6 cut(s) 31, 82, 167, 282, 312, 573
HpyAV CCTTC 2 cut(s) 107, 631
HpyCH4III ACNGT 3 cut(s) 171, 259, 362
HpyCH4V TGCA 4 cut(s) 208, 426, 691, 755
Hsp92II CATG 6 cut(s) 151, 353, 417, 541, 658, 740
KpnI GGTACC 1 cut(s) 120
KspAI GTTAAC 1 cut(s) 282
Kzo9I GATC 2 cut(s) 400, 742
LmnI GCTCC 3 cut(s) 135, 466, 478
LpnPI CCDG 8 cut(s) 51, 112, 127, 237, 248, 264, 489, 537
MabI ACCWGGT 1 cut(s) 250
MaeI CTAG 3 cut(s) 161, 272, 650
MaeIII GTNAC 1 cut(s) 529
MalI GATC 2 cut(s) 402, 744
MboI GATC 2 cut(s) 400, 742
MboII GAAGA 5 cut(s) 82, 480, 494, 644, 752
MhlI GDGCHC 1 cut(s) 628
MluCI AATT 4 cut(s) 74, 187, 671, 757
MmeI TCCRAC 1 cut(s) 133
MnlI CCTC 7 cut(s) 98, 143, 242, 505, 508, 655, 719
MseI TTAA 3 cut(s) 281, 606, 645
MslI CAYNNNNRTG 1 cut(s) 542
MspI CCGG 3 cut(s) 114, 476, 524
MspR9I CCNGG 1 cut(s) 252
MvaI CCWGG 1 cut(s) 252
NdeII GATC 2 cut(s) 400, 742
NlaIII CATG 6 cut(s) 151, 353, 417, 541, 658, 740
NlaIV GGNNCC 3 cut(s) 118, 131, 625
NspI RCATGY 1 cut(s) 417
PciI ACATGT 1 cut(s) 413
PfeI GAWTC 2 cut(s) 485, 683
PinAI ACCGGT 1 cut(s) 523
PscI ACATGT 1 cut(s) 413
Psp6I CCWGG 1 cut(s) 250
PspGI CCWGG 1 cut(s) 250
PspN4I GGNNCC 3 cut(s) 118, 131, 625
RsaI GTAC 3 cut(s) 118, 173, 256
RsaNI GTAC 3 cut(s) 117, 172, 255
RseI CAYNNNNRTG 1 cut(s) 542
SalI GTCGAC 1 cut(s) 310
SaqAI TTAA 3 cut(s) 281, 606, 645
Sau3AI GATC 2 cut(s) 400, 742
ScrFI CCNGG 1 cut(s) 252
SduI GDGCHC 1 cut(s) 628
SexAI ACCWGGT 1 cut(s) 250
SfcI CTRYAG 3 cut(s) 358, 367, 585
SmiMI CAYNNNNRTG 1 cut(s) 542
SmlI CTYRAG 1 cut(s) 677
SmoI CTYRAG 1 cut(s) 677
Sse9I AATT 4 cut(s) 74, 187, 671, 757
SsiI CCGC 2 cut(s) 111, 492
SspMI CTAG 3 cut(s) 161, 272, 650
StyD4I CCNGG 1 cut(s) 250
StyI CCWWGG 2 cut(s) 160, 271
TaaI ACNGT 3 cut(s) 171, 259, 362
TaqI TCGA 1 cut(s) 311
TasI AATT 4 cut(s) 74, 187, 671, 757
TfiI GAWTC 2 cut(s) 485, 683
Tru1I TTAA 3 cut(s) 281, 606, 645
Tru9I TTAA 3 cut(s) 281, 606, 645
TscAI CASTG 1 cut(s) 367
TspDTI ATGAA 1 cut(s) 753
TspRI CASTG 1 cut(s) 367
XagI CCTNNNNNAGG 2 cut(s) 137, 502
XapI RAATTY 2 cut(s) 74, 187
XceI RCATGY 1 cut(s) 417
XmaJI CCTAGG 2 cut(s) 160, 271
XmiI GTMKAC 2 cut(s) 311, 572
XspI CTAG 3 cut(s) 161, 272, 650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.