Rw6G003190

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000352
Physical Location & Seq
Forward (+)
5157574 .. 5158397
824 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G003190.1

Sequence Viewer

Length: 675 bp
ATGTCCCAAAAAACTCCACCCCCGAAGGTTAGTTTGAAACTCCTAATCGACAAAGAGTATCATAAGGTTGTGTTTGCTGAAGCTGGCAAAGAGTTCGTGGATTTCCTCTTCACTCTTCTGTCTCTGCCTCTTTTAAATACGGCCAGAGTTTGTAAGGTTGGAAGTTTAAGCAATATCTATGGGAGCATTGACGCCCTCGGTGACAAGTACATGCTCCCCAATGTCAACAAGAACACTATGCTGAGATCCAAGGAACATGCTGCTGTATTTAACAAACTTCAGCTTGTGTTGAGAAACGAACCGGACCTTAAACCAATTATGCCGTCGAATGTGAAGAAGTTTTATATGTGTGAGAATTTCAAGTTGAAGCAACACGGCTCATGGGTGACAGATGACTCTAAAGCCATTTGTCCAATGTGCAGTACAACTATGTCGACCGGTGTCGGCTATGTTGCTCCTCCGGGAGAACCTAGTTTGATGACCAGGGACGAAGGGTATGTGAGAGGTGTTGCTTCATACATGATAATGGACGATCTGAGAGTCAAACCTTTGTCTGTCTTTTCAAATTTGAATTTGCTTAAGAAGTGCATCAATGGCAAGAAATGGTTGAAGGCATTAATGGAGTCAAACTCAGTTCTTACCGATCTCTTCCTTGGGAAGAAACCAATTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

224

Amino Acids

25.04

Weight (kDa)

9.4

Isoelectric Point (pI)

32.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 8 - 196 4.4e-24 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000352)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g24890 FvH4_7g21280 FvH4_7g21300 FvH4_7g21300 FvH4_7g21310 FvH4_7g21330 FvH4_7g21340 FvH4_7g21600 FvH4_7g21610 FvH4_7g21630
malus_domestica MD07G1190400.v1.1 MD07G1190600.v1.1
prunus_persica Prupe.2G228600_v2.0.a1 Prupe.6G198200_v2.0.a1
pyrus_communis pycom07g18010
rosa_chinensis RchiOBHm_Chr1g0365711 RchiOBHm_Chr1g0365721 RchiOBHm_Chr1g0365731 RchiOBHm_Chr1g0365991 RchiOBHm_Chr5g0044511 RchiOBHm_Chr6g0247041 RchiOBHm_Chr6g0247081
rosa_laevigata RLG00000015223 RLG00000027405 RLG00000027406 RLG00000027407 RLG00000027408 RLG00000027409 RLG00000027411 RLG00000027413 RLG00000034277
rosa_multiflora Rmu_co8279495.1_g000001 Rmu_co8358963.1_g000001 Rmu_co8520123.1_g000003 Rmu_sc0002093.1_g000019 Rmu_sc0002093.1_g000024 Rmu_sc0002093.1_g000025 Rmu_sc0002093.1_g000029 Rmu_sc0002476.1_g000002 Rmu_sc0002476.1_g000021 Rmu_sc0003466.1_g000011 Rmu_sc0004288.1_g000006 Rmu_sc0004288.1_g000007 Rmu_sc0004311.1_g000022 Rmu_sc0004311.1_g000023 Rmu_sc0004311.1_g000026 Rmu_sc0004311.1_g000033 Rmu_sc0004577.1_g000008 Rmu_sc0004645.1_g000005 Rmu_sc0004645.1_g000006 Rmu_sc0006406.1_g000011 Rmu_sc0006406.1_g000012 Rmu_sc0009967.1_g000002 Rmu_sc0011598.1_g000003 Rmu_ssc0000151.1_g000015
rosa_roxburghii Rroxscaffold_1G00036500 Rroxscaffold_3G00276050 Rroxscaffold_4G00290950 Rroxscaffold_4G00290960 Rroxscaffold_4G00290970 Rroxscaffold_4G00290980 Rroxscaffold_4G00291000 Rroxscaffold_4G00291030 Rroxscaffold_4G00291060
rosa_rugosa Rorug01G0322900 Rorug01G0323000 Rorug01G0323100 Rorug01G0323200 Rorug01G0323300 Rorug01G0323400 Rorug01G0323500 Rorug01G0323600 Rorug05G0214700 Rorug05G0522100
rosa_samantha Rh1AG332000 Rh1AG332200 Rh1AG332300 Rh1AG334700 Rh1AG334800 Rh1AG335200 Rh1CG309900 Rh1CG310000 Rh1CG310100 Rh1DG325100 Rh1DG325300 Rh1DG325500 Rh5AG299000 Rh5BG306500 Rh5DG315800 Rh6AG037200 Rh6BG031900 Rh6BG032000 Rh6CG032300 Rh6CG032400 Rh6CG033200 Rh6DG030900
rosa_wichuraiana Rw0G001530 Rw1G019070 Rw1G029470 Rw1G029480 Rw1G029490 Rw1G029500 Rw1G029510 Rw1G029700 Rw5G027640 Rw5G027670 Rw6G003160 Rw6G003190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 434
AclWI GGATC 1 cut(s) 240
AcoI YGGCCR 1 cut(s) 141
AcsI RAATTY 3 cut(s) 355, 565, 571
AcuI CTGAAG 2 cut(s) 99, 263
AcyI GRCGYC 1 cut(s) 192
AfaI GTAC 2 cut(s) 209, 424
AflII CTTAAG 1 cut(s) 578
AgeI ACCGGT 1 cut(s) 437
AgsI TTSAA 6 cut(s) 37, 361, 367, 564, 571, 610
AjnI CCWGG 1 cut(s) 482
AluBI AGCT 2 cut(s) 83, 283
AluI AGCT 2 cut(s) 83, 283
Alw26I GTCTC 1 cut(s) 126
AlwI GGATC 1 cut(s) 240
AoxI GGCC 1 cut(s) 141
ApeKI GCWGC 1 cut(s) 260
ApoI RAATTY 3 cut(s) 355, 565, 571
AseI ATTAAT 1 cut(s) 617
AsiGI ACCGGT 1 cut(s) 437
AspS9I GGNCC 1 cut(s) 304
AsuC2I CCSGG 1 cut(s) 462
AsuHPI GGTGA 2 cut(s) 212, 397
AvaII GGWCC 1 cut(s) 304
BbvI GCAGC 1 cut(s) 247
BceAI ACGGC 3 cut(s) 156, 307, 391
BcgI CGANNNNNNTGC 2 cut(s) 76, 110
BciT130I CCWGG 1 cut(s) 484
BcnI CCSGG 1 cut(s) 462
BcoDI GTCTC 1 cut(s) 126
BfaI CTAG 1 cut(s) 471
BfrI CTTAAG 1 cut(s) 578
BisI GCNGC 1 cut(s) 261
BlsI GCNGC 1 cut(s) 262
Bme1390I CCNGG 2 cut(s) 462, 484
Bme18I GGWCC 1 cut(s) 304
BmgT120I GGNCC 1 cut(s) 304
BmrFI CCNGG 2 cut(s) 462, 484
BmsI GCATC 1 cut(s) 597
BoxI GACNNNNGTC 1 cut(s) 440
BplI GAGNNNNNCTC 2 cut(s) 614, 646
BpuMI CCSGG 1 cut(s) 462
BsaHI GRCGYC 1 cut(s) 192
BsaJI CCNNGG 4 cut(s) 196, 249, 483, 652
BsaWI WCCGGW 2 cut(s) 301, 437
Bse118I RCCGGY 1 cut(s) 437
BseBI CCWGG 1 cut(s) 484
BseDI CCNNGG 4 cut(s) 196, 249, 483, 652
BseMII CTCAG 3 cut(s) 233, 527, 645
BseRI GAGGAG 1 cut(s) 447
BseXI GCAGC 1 cut(s) 247
BsgI GTGCAG 1 cut(s) 439
Bsh1285I CGRYCG 1 cut(s) 438
BshFI GGCC 1 cut(s) 143
BshTI ACCGGT 1 cut(s) 437
BsiEI CGRYCG 1 cut(s) 438
BsiSI CCGG 3 cut(s) 302, 438, 461
BslFI GGGAC 1 cut(s) 500
BsmAI GTCTC 1 cut(s) 126
BsmFI GGGAC 1 cut(s) 500
BsnI GGCC 1 cut(s) 143
Bsp143I GATC 3 cut(s) 245, 532, 643
BspANI GGCC 1 cut(s) 143
BspCNI CTCAG 3 cut(s) 234, 528, 644
BspPI GGATC 1 cut(s) 240
BspTI CTTAAG 1 cut(s) 578
BsrFI RCCGGY 1 cut(s) 437
BssAI RCCGGY 1 cut(s) 437
BssECI CCNNGG 4 cut(s) 196, 249, 483, 652
BssMI GATC 3 cut(s) 245, 532, 643
BssNI GRCGYC 1 cut(s) 192
BssT1I CCWWGG 2 cut(s) 249, 652
Bst2UI CCWGG 1 cut(s) 484
Bst6I CTCTTC 3 cut(s) 113, 120, 653
BstACI GRCGYC 1 cut(s) 192
BstAFI CTTAAG 1 cut(s) 578
BstC8I GCNNGC 1 cut(s) 85
BstDEI CTNAG 3 cut(s) 242, 536, 631
BstKTI GATC 3 cut(s) 248, 535, 646
BstMAI GTCTC 1 cut(s) 126
BstMBI GATC 3 cut(s) 245, 532, 643
BstMCI CGRYCG 1 cut(s) 438
BstMWI GCNNNNNNNGC 1 cut(s) 594
BstNI CCWGG 1 cut(s) 484
BstNSI RCATGY 2 cut(s) 214, 260
BstPAI GACNNNNGTC 1 cut(s) 440
BstSCI CCNGG 2 cut(s) 460, 482
BstV1I GCAGC 1 cut(s) 247
BstX2I RGATCY 1 cut(s) 245
BstYI RGATCY 1 cut(s) 245
BsuRI GGCC 1 cut(s) 143
Cac8I GCNNGC 1 cut(s) 85
Cfr10I RCCGGY 1 cut(s) 437
Cfr13I GGNCC 1 cut(s) 304
CseI GACGC 1 cut(s) 200
Csp6I GTAC 2 cut(s) 208, 423
CspAI ACCGGT 1 cut(s) 437
CviAII CATG 4 cut(s) 211, 257, 381, 520
CviJI RGCY 6 cut(s) 83, 143, 283, 378, 404, 447
CviKI_1 RGCY 6 cut(s) 83, 143, 283, 378, 404, 447
CviQI GTAC 2 cut(s) 208, 423
DdeI CTNAG 3 cut(s) 242, 536, 631
DpnI GATC 3 cut(s) 247, 534, 645
DpnII GATC 3 cut(s) 245, 532, 643
DraI TTTAAA 1 cut(s) 135
EaeI YGGCCR 1 cut(s) 141
Eam1104I CTCTTC 3 cut(s) 113, 120, 653
EarI CTCTTC 3 cut(s) 113, 120, 653
Eco130I CCWWGG 2 cut(s) 249, 652
Eco47I GGWCC 1 cut(s) 304
Eco57I CTGAAG 2 cut(s) 99, 263
EcoRII CCWGG 1 cut(s) 482
EcoT14I CCWWGG 2 cut(s) 249, 652
ErhI CCWWGG 2 cut(s) 249, 652
FaeI CATG 4 cut(s) 214, 260, 384, 523
FaqI GGGAC 1 cut(s) 500
FatI CATG 4 cut(s) 210, 256, 380, 519
FblI GTMKAC 1 cut(s) 434
Fnu4HI GCNGC 1 cut(s) 261
Fsp4HI GCNGC 1 cut(s) 261
FspBI CTAG 1 cut(s) 471
GluI GCNGC 1 cut(s) 261
HaeIII GGCC 1 cut(s) 143
HapII CCGG 3 cut(s) 302, 438, 461
HgaI GACGC 1 cut(s) 200
Hin1I GRCGYC 1 cut(s) 192
Hin1II CATG 4 cut(s) 214, 260, 384, 523
HincII GTYRAC 2 cut(s) 226, 435
HindII GTYRAC 2 cut(s) 226, 435
HinfI GANTC 3 cut(s) 395, 540, 623
HpaII CCGG 3 cut(s) 302, 438, 461
HphI GGTGA 2 cut(s) 212, 397
Hpy166II GTNNAC 2 cut(s) 226, 435
Hpy188I TCNGA 1 cut(s) 537
Hpy8I GTNNAC 2 cut(s) 226, 435
Hpy99I CGWCG 1 cut(s) 328
HpyAV CCTTC 3 cut(s) 19, 485, 604
HpyCH4V TGCA 2 cut(s) 420, 588
HpyF10VI GCNNNNNNNGC 1 cut(s) 594
HpyF3I CTNAG 3 cut(s) 242, 536, 631
Hsp92I GRCGYC 1 cut(s) 192
Hsp92II CATG 4 cut(s) 214, 260, 384, 523
Kzo9I GATC 3 cut(s) 245, 532, 643
LmnI GCTCC 3 cut(s) 183, 219, 460
LpnPI CCDG 7 cut(s) 69, 157, 315, 451, 469, 474, 496
Lsp1109I GCAGC 1 cut(s) 247
LweI GCATC 1 cut(s) 597
MaeI CTAG 1 cut(s) 471
MaeIII GTNAC 2 cut(s) 200, 385
MalI GATC 3 cut(s) 247, 534, 645
MboI GATC 3 cut(s) 245, 532, 643
MboII GAAGA 5 cut(s) 100, 107, 346, 640, 670
MfeI CAATTG 1 cut(s) 666
MflI RGATCY 1 cut(s) 245
MluCI AATT 5 cut(s) 315, 355, 565, 571, 666
MlyI GAGTC 3 cut(s) 389, 549, 632
MmeI TCCRAC 1 cut(s) 139
MnlI CCTC 5 cut(s) 116, 138, 206, 468, 497
MseI TTAA 6 cut(s) 134, 167, 270, 309, 579, 617
MslI CAYNNNNRTG 1 cut(s) 524
MspCI CTTAAG 1 cut(s) 578
MspI CCGG 3 cut(s) 302, 438, 461
MspR9I CCNGG 2 cut(s) 462, 484
MunI CAATTG 1 cut(s) 666
MvaI CCWGG 1 cut(s) 484
MwoI GCNNNNNNNGC 1 cut(s) 594
NciI CCSGG 1 cut(s) 462
NdeII GATC 3 cut(s) 245, 532, 643
NlaIII CATG 4 cut(s) 214, 260, 384, 523
NmuCI GTSAC 2 cut(s) 200, 385
NspI RCATGY 2 cut(s) 214, 260
PfoI TCCNGGA 1 cut(s) 460
PinAI ACCGGT 1 cut(s) 437
PkrI GCNGC 1 cut(s) 262
PleI GAGTC 3 cut(s) 389, 548, 631
PpsI GAGTC 3 cut(s) 389, 548, 631
PshAI GACNNNNGTC 1 cut(s) 440
PshBI ATTAAT 1 cut(s) 617
Psp6I CCWGG 1 cut(s) 482
PspGI CCWGG 1 cut(s) 482
PspPI GGNCC 1 cut(s) 304
PsuI RGATCY 1 cut(s) 245
RsaI GTAC 2 cut(s) 209, 424
RsaNI GTAC 2 cut(s) 208, 423
RseI CAYNNNNRTG 1 cut(s) 524
SalI GTCGAC 1 cut(s) 433
SaqAI TTAA 6 cut(s) 134, 167, 270, 309, 579, 617
SatI GCNGC 1 cut(s) 261
Sau3AI GATC 3 cut(s) 245, 532, 643
Sau96I GGNCC 1 cut(s) 304
SchI GAGTC 3 cut(s) 389, 549, 632
ScrFI CCNGG 2 cut(s) 462, 484
SetI ASST 9 cut(s) 30, 69, 85, 159, 285, 309, 472, 508, 550
SfaNI GCATC 1 cut(s) 597
SinI GGWCC 1 cut(s) 304
SmiMI CAYNNNNRTG 1 cut(s) 524
SmlI CTYRAG 1 cut(s) 578
SmoI CTYRAG 1 cut(s) 578
Sse9I AATT 5 cut(s) 315, 355, 565, 571, 666
SspMI CTAG 1 cut(s) 471
StyD4I CCNGG 2 cut(s) 460, 482
StyI CCWWGG 2 cut(s) 249, 652
TaqI TCGA 3 cut(s) 48, 326, 434
TasI AATT 5 cut(s) 315, 355, 565, 571, 666
TatI WGTACW 2 cut(s) 207, 422
Tru1I TTAA 6 cut(s) 134, 167, 270, 309, 579, 617
Tru9I TTAA 6 cut(s) 134, 167, 270, 309, 579, 617
TseFI GTSAC 2 cut(s) 200, 385
TseI GCWGC 1 cut(s) 260
Tsp45I GTSAC 2 cut(s) 200, 385
TspDTI ATGAA 1 cut(s) 504
Vha464I CTTAAG 1 cut(s) 578
VpaK11BI GGWCC 1 cut(s) 304
VspI ATTAAT 1 cut(s) 617
XapI RAATTY 3 cut(s) 355, 565, 571
XceI RCATGY 2 cut(s) 214, 260
XmiI GTMKAC 1 cut(s) 434
XspI CTAG 1 cut(s) 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.