Rroxscaffold_1G00036500

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000352
Physical Location & Seq
Reverse (-)
53259304 .. 53260284
981 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00036500.1

Sequence Viewer

Length: 642 bp
ATGGCCGACTCTAAATCTACAGTCAGCCTGAAGCTACTCATCGATACAAAGCGTCAAAAGGTTCTGTTTGCTGAAGCGGGTAAGGACTTTGTGGATTTTCTTTTCACCCTTCTCTCTTTGCCAGTTGGCACTGTTATTAGGCTTCTGTCAAAGGATGGCATGGTTGGTAGCTTTGGTAAACTGTATGACAGTGTTGAAAATCTAGATTTAACATATATGGAACCCAAACTAAACAAGGATATCCTGCTCAAACCCAAGGGACCTGTTGCTGGACCTAATATCTTCGGTTTGTTGGACTTGGCCAACGTTGACGAACCAGCTGCTAGAAAAAGGCGTGGACAGTGTAACTTTTCCGGCTCTGCGCCTACCGCCCACCTTTATGTGACTGAAGATCCTAACGAACAATGTCCAGAGTGCAATCACCATATGTATTCTCAGGCGACTTATGTTGGTCAACCAAAGACCACAGTAGCAGCTTCTCGCTTCGGTGCAGCAGGATATGTCAAAGGCCTAGATGTTGGCACCCTCGAAGAGAAGGTTGTAGATCTTGGCACGAATGAGGGTGTGAAACTGCTGAAAGAATCCCTACAATCCAAAGCAGTTCTCACAAATGTGTTCCTGGGGAAGAAAGCTGCTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

213

Amino Acids

23.01

Weight (kDa)

8.32

Isoelectric Point (pI)

31.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 5 - 143 2.2e-26 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000352)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g24890 FvH4_7g21280 FvH4_7g21300 FvH4_7g21300 FvH4_7g21310 FvH4_7g21330 FvH4_7g21340 FvH4_7g21600 FvH4_7g21610 FvH4_7g21630
malus_domestica MD07G1190400.v1.1 MD07G1190600.v1.1
prunus_persica Prupe.2G228600_v2.0.a1 Prupe.6G198200_v2.0.a1
pyrus_communis pycom07g18010
rosa_chinensis RchiOBHm_Chr1g0365711 RchiOBHm_Chr1g0365721 RchiOBHm_Chr1g0365731 RchiOBHm_Chr1g0365991 RchiOBHm_Chr5g0044511 RchiOBHm_Chr6g0247041 RchiOBHm_Chr6g0247081
rosa_laevigata RLG00000015223 RLG00000027405 RLG00000027406 RLG00000027407 RLG00000027408 RLG00000027409 RLG00000027411 RLG00000027413 RLG00000034277
rosa_multiflora Rmu_co8279495.1_g000001 Rmu_co8358963.1_g000001 Rmu_co8520123.1_g000003 Rmu_sc0002093.1_g000019 Rmu_sc0002093.1_g000024 Rmu_sc0002093.1_g000025 Rmu_sc0002093.1_g000029 Rmu_sc0002476.1_g000002 Rmu_sc0002476.1_g000021 Rmu_sc0003466.1_g000011 Rmu_sc0004288.1_g000006 Rmu_sc0004288.1_g000007 Rmu_sc0004311.1_g000022 Rmu_sc0004311.1_g000023 Rmu_sc0004311.1_g000026 Rmu_sc0004311.1_g000033 Rmu_sc0004577.1_g000008 Rmu_sc0004645.1_g000005 Rmu_sc0004645.1_g000006 Rmu_sc0006406.1_g000011 Rmu_sc0006406.1_g000012 Rmu_sc0009967.1_g000002 Rmu_sc0011598.1_g000003 Rmu_ssc0000151.1_g000015
rosa_roxburghii Rroxscaffold_1G00036500 Rroxscaffold_3G00276050 Rroxscaffold_4G00290950 Rroxscaffold_4G00290960 Rroxscaffold_4G00290970 Rroxscaffold_4G00290980 Rroxscaffold_4G00291000 Rroxscaffold_4G00291030 Rroxscaffold_4G00291060
rosa_rugosa Rorug01G0322900 Rorug01G0323000 Rorug01G0323100 Rorug01G0323200 Rorug01G0323300 Rorug01G0323400 Rorug01G0323500 Rorug01G0323600 Rorug05G0214700 Rorug05G0522100
rosa_samantha Rh1AG332000 Rh1AG332200 Rh1AG332300 Rh1AG334700 Rh1AG334800 Rh1AG335200 Rh1CG309900 Rh1CG310000 Rh1CG310100 Rh1DG325100 Rh1DG325300 Rh1DG325500 Rh5AG299000 Rh5BG306500 Rh5DG315800 Rh6AG037200 Rh6BG031900 Rh6BG032000 Rh6CG032300 Rh6CG032400 Rh6CG033200 Rh6DG030900
rosa_wichuraiana Rw0G001530 Rw1G019070 Rw1G029470 Rw1G029480 Rw1G029490 Rw1G029500 Rw1G029510 Rw1G029700 Rw5G027640 Rw5G027670 Rw6G003160 Rw6G003190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 521
AciI CCGC 2 cut(s) 77, 369
AclI AACGTT 1 cut(s) 306
AclWI GGATC 1 cut(s) 386
AcoI YGGCCR 2 cut(s) 3, 300
AcuI CTGAAG 3 cut(s) 50, 93, 408
AfiI CCNNNNNNNGG 1 cut(s) 269
AgsI TTSAA 1 cut(s) 197
AjnI CCWGG 1 cut(s) 618
AleI CACNNNNGTG 1 cut(s) 611
AluBI AGCT 5 cut(s) 34, 171, 320, 476, 632
AluI AGCT 5 cut(s) 34, 171, 320, 476, 632
AlwI GGATC 1 cut(s) 386
AoxI GGCC 3 cut(s) 3, 300, 508
ApeKI GCWGC 5 cut(s) 320, 473, 491, 632, 635
AspLEI GCGC 1 cut(s) 364
AspS9I GGNCC 2 cut(s) 260, 272
AsuHPI GGTGA 2 cut(s) 97, 413
AvaII GGWCC 2 cut(s) 260, 272
BalI TGGCCA 1 cut(s) 302
BanI GGYRCC 1 cut(s) 521
BbvI GCAGC 5 cut(s) 307, 485, 503, 619, 622
BccI CCATC 1 cut(s) 149
BcgI CGANNNNNNTGC 2 cut(s) 302, 336
BciT130I CCWGG 1 cut(s) 620
BfaI CTAG 3 cut(s) 203, 324, 512
BfmI CTRYAG 1 cut(s) 18
BglII AGATCT 1 cut(s) 544
BisI GCNGC 5 cut(s) 321, 474, 492, 633, 636
BlsI GCNGC 5 cut(s) 322, 475, 493, 634, 637
Bme1390I CCNGG 1 cut(s) 620
Bme18I GGWCC 2 cut(s) 260, 272
BmgT120I GGNCC 2 cut(s) 260, 272
BmiI GGNNCC 3 cut(s) 222, 261, 523
BmrFI CCNGG 1 cut(s) 620
Bsa29I ATCGAT 1 cut(s) 42
BsaJI CCNNGG 2 cut(s) 255, 619
Bsc4I CCNNNNNNNGG 1 cut(s) 269
Bse1I ACTGG 1 cut(s) 122
BseBI CCWGG 1 cut(s) 620
BseCI ATCGAT 1 cut(s) 42
BseDI CCNNGG 2 cut(s) 255, 619
BseGI GGATG 1 cut(s) 160
BseLI CCNNNNNNNGG 1 cut(s) 269
BseMII CTCAG 1 cut(s) 449
BseNI ACTGG 1 cut(s) 122
BseXI GCAGC 5 cut(s) 307, 485, 503, 619, 622
BsgI GTGCAG 1 cut(s) 510
BshFI GGCC 3 cut(s) 5, 302, 510
BshNI GGYRCC 1 cut(s) 521
BshVI ATCGAT 1 cut(s) 42
BsiSI CCGG 1 cut(s) 354
BslFI GGGAC 1 cut(s) 273
BslI CCNNNNNNNGG 1 cut(s) 269
BsmFI GGGAC 1 cut(s) 273
BsnI GGCC 3 cut(s) 5, 302, 510
Bsp143I GATC 2 cut(s) 391, 544
BspACI CCGC 2 cut(s) 77, 369
BspANI GGCC 3 cut(s) 5, 302, 510
BspCNI CTCAG 1 cut(s) 448
BspDI ATCGAT 1 cut(s) 42
BspLI GGNNCC 3 cut(s) 222, 261, 523
BspPI GGATC 1 cut(s) 386
BspT107I GGYRCC 1 cut(s) 521
BsrI ACTGG 1 cut(s) 122
BssECI CCNNGG 2 cut(s) 255, 619
BssMI GATC 2 cut(s) 391, 544
BssT1I CCWWGG 1 cut(s) 255
Bst2UI CCWGG 1 cut(s) 620
Bst4CI ACNGT 6 cut(s) 22, 133, 183, 191, 342, 469
Bst6I CTCTTC 1 cut(s) 525
BstDEI CTNAG 1 cut(s) 435
BstF5I GGATG 1 cut(s) 160
BstHHI GCGC 1 cut(s) 364
BstKTI GATC 2 cut(s) 394, 547
BstMBI GATC 2 cut(s) 391, 544
BstMWI GCNNNNNNNGC 1 cut(s) 368
BstNI CCWGG 1 cut(s) 620
BstSCI CCNGG 1 cut(s) 618
BstSFI CTRYAG 1 cut(s) 18
BstV1I GCAGC 5 cut(s) 307, 485, 503, 619, 622
BstX2I RGATCY 2 cut(s) 391, 544
BstYI RGATCY 2 cut(s) 391, 544
Bsu15I ATCGAT 1 cut(s) 42
BsuRI GGCC 3 cut(s) 5, 302, 510
BsuTUI ATCGAT 1 cut(s) 42
BtsCI GGATG 1 cut(s) 160
BtsIMutI CAGTG 3 cut(s) 129, 196, 347
CfoI GCGC 1 cut(s) 364
Cfr13I GGNCC 2 cut(s) 260, 272
ClaI ATCGAT 1 cut(s) 42
CseI GACGC 1 cut(s) 41
CviAII CATG 2 cut(s) 160, 639
DdeI CTNAG 1 cut(s) 435
DpnI GATC 2 cut(s) 393, 546
DpnII GATC 2 cut(s) 391, 544
EaeI YGGCCR 2 cut(s) 3, 300
Eam1104I CTCTTC 1 cut(s) 525
EarI CTCTTC 1 cut(s) 525
Eco130I CCWWGG 1 cut(s) 255
Eco147I AGGCCT 1 cut(s) 510
Eco32I GATATC 1 cut(s) 241
Eco47I GGWCC 2 cut(s) 260, 272
Eco57I CTGAAG 3 cut(s) 50, 93, 408
EcoO109I RGGNCCY 1 cut(s) 260
EcoRII CCWGG 1 cut(s) 618
EcoRV GATATC 1 cut(s) 241
EcoT14I CCWWGG 1 cut(s) 255
ErhI CCWWGG 1 cut(s) 255
FaeI CATG 2 cut(s) 163, 642
FaqI GGGAC 1 cut(s) 273
FatI CATG 2 cut(s) 159, 638
FauI CCCGC 1 cut(s) 70
FauNDI CATATG 1 cut(s) 426
Fnu4HI GCNGC 5 cut(s) 321, 474, 492, 633, 636
FokI GGATG 1 cut(s) 167
Fsp4HI GCNGC 5 cut(s) 321, 474, 492, 633, 636
FspBI CTAG 3 cut(s) 203, 324, 512
GlaI GCGC 1 cut(s) 363
GluI GCNGC 5 cut(s) 321, 474, 492, 633, 636
HaeIII GGCC 3 cut(s) 5, 302, 510
HapII CCGG 1 cut(s) 354
HgaI GACGC 1 cut(s) 41
HhaI GCGC 1 cut(s) 364
Hin1II CATG 2 cut(s) 163, 642
Hin6I GCGC 1 cut(s) 362
HinP1I GCGC 1 cut(s) 362
HincII GTYRAC 2 cut(s) 310, 455
HindII GTYRAC 2 cut(s) 310, 455
HinfI GANTC 2 cut(s) 8, 581
HpaII CCGG 1 cut(s) 354
HphI GGTGA 2 cut(s) 97, 413
Hpy166II GTNNAC 4 cut(s) 179, 310, 338, 455
Hpy188III TCNNGA 2 cut(s) 203, 410
Hpy8I GTNNAC 4 cut(s) 179, 310, 338, 455
HpyAV CCTTC 2 cut(s) 119, 529
HpyCH4III ACNGT 6 cut(s) 22, 133, 183, 191, 342, 469
HpyCH4IV ACGT 1 cut(s) 306
HpyCH4V TGCA 3 cut(s) 417, 491, 638
HpyF10VI GCNNNNNNNGC 1 cut(s) 368
HpyF3I CTNAG 1 cut(s) 435
HpySE526I ACGT 1 cut(s) 306
Hsp92II CATG 2 cut(s) 163, 642
HspAI GCGC 1 cut(s) 362
Kzo9I GATC 2 cut(s) 391, 544
Lsp1109I GCAGC 5 cut(s) 307, 485, 503, 619, 622
MaeI CTAG 3 cut(s) 203, 324, 512
MaeII ACGT 1 cut(s) 306
MaeIII GTNAC 2 cut(s) 344, 382
MalI GATC 2 cut(s) 393, 546
MboI GATC 2 cut(s) 391, 544
MboII GAAGA 4 cut(s) 274, 401, 542, 637
MflI RGATCY 2 cut(s) 391, 544
MlsI TGGCCA 1 cut(s) 302
MluNI TGGCCA 1 cut(s) 302
MlyI GAGTC 1 cut(s) 2
MmeI TCCRAC 1 cut(s) 273
MnlI CCTC 2 cut(s) 536, 553
Mox20I TGGCCA 1 cut(s) 302
MscI TGGCCA 1 cut(s) 302
MseI TTAA 1 cut(s) 209
MslI CAYNNNNRTG 2 cut(s) 378, 611
Msp20I TGGCCA 1 cut(s) 302
MspA1I CMGCKG 1 cut(s) 320
MspI CCGG 1 cut(s) 354
MspR9I CCNGG 1 cut(s) 620
MvaI CCWGG 1 cut(s) 620
MwoI GCNNNNNNNGC 1 cut(s) 368
NdeI CATATG 1 cut(s) 426
NdeII GATC 2 cut(s) 391, 544
NlaIII CATG 2 cut(s) 163, 642
NlaIV GGNNCC 3 cut(s) 222, 261, 523
NmuCI GTSAC 1 cut(s) 382
OliI CACNNNNGTG 1 cut(s) 611
PceI AGGCCT 1 cut(s) 510
PfeI GAWTC 1 cut(s) 581
PkrI GCNGC 5 cut(s) 322, 475, 493, 634, 637
PleI GAGTC 1 cut(s) 2
PpsI GAGTC 1 cut(s) 2
PpuMI RGGWCCY 1 cut(s) 260
Psp1406I AACGTT 1 cut(s) 306
Psp5II RGGWCCY 1 cut(s) 260
Psp6I CCWGG 1 cut(s) 618
PspGI CCWGG 1 cut(s) 618
PspN4I GGNNCC 3 cut(s) 222, 261, 523
PspPI GGNCC 2 cut(s) 260, 272
PspPPI RGGWCCY 1 cut(s) 260
PsuI RGATCY 2 cut(s) 391, 544
PvuII CAGCTG 1 cut(s) 320
RseI CAYNNNNRTG 2 cut(s) 378, 611
SaqAI TTAA 1 cut(s) 209
SatI GCNGC 5 cut(s) 321, 474, 492, 633, 636
Sau3AI GATC 2 cut(s) 391, 544
Sau96I GGNCC 2 cut(s) 260, 272
SchI GAGTC 1 cut(s) 2
ScrFI CCNGG 1 cut(s) 620
SfcI CTRYAG 1 cut(s) 18
SinI GGWCC 2 cut(s) 260, 272
SmiMI CAYNNNNRTG 2 cut(s) 378, 611
SseBI AGGCCT 1 cut(s) 510
SsiI CCGC 2 cut(s) 77, 369
SspMI CTAG 3 cut(s) 203, 324, 512
StuI AGGCCT 1 cut(s) 510
StyD4I CCNGG 1 cut(s) 618
StyI CCWWGG 1 cut(s) 255
TaaI ACNGT 6 cut(s) 22, 133, 183, 191, 342, 469
TaiI ACGT 1 cut(s) 309
TaqI TCGA 2 cut(s) 42, 528
TfiI GAWTC 1 cut(s) 581
Tru1I TTAA 1 cut(s) 209
Tru9I TTAA 1 cut(s) 209
TscAI CASTG 3 cut(s) 136, 196, 347
TseFI GTSAC 1 cut(s) 382
TseI GCWGC 5 cut(s) 320, 473, 491, 632, 635
Tsp45I GTSAC 1 cut(s) 382
TspRI CASTG 3 cut(s) 136, 196, 347
VpaK11BI GGWCC 2 cut(s) 260, 272
XbaI TCTAGA 1 cut(s) 202
XspI CTAG 3 cut(s) 203, 324, 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.