FvH4_7g21300

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000352
Physical Location & Seq
Forward (+)
17104079 .. 17105418
1340 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g21300.t1

Sequence Viewer

Length: 717 bp
ATGGCAACCTCTTCCAGCTCCACTGTTGGACTGAAGCTCCTCATCGATACAAAGCAAGAAAAGGTTCTCTTTGCCGAAGCTGGTAAGGACTTTGTCGATTTTCTCTTCAGTCTGCTCTCTTTGCCTCTAGGCACAGTCATCAGGCTTCTCGACAAGGATGGCATGGTGGGTGGCTTTGGAAAACTCTATCAGAGTGTAAAAAAACTACATCATACATACATGCAACCCAACAGTAACAAGGACGTGCTTCTTAAACCTAAGGCACCACTTGGATCCAACATCCTTAAGCTGAGCAACACTGAATCATGCTCTCGGAAAGCGATCTACATGTGTGCGAGTGGGAAAGATCACAAGTATGTGTCCGAGGACCCCAAAGCTAAGTGTCCATCCTGTAATCAAACTTTGAGCGCGCAGACCACTTATGTTGCTTCACCGTCGACTACCTCAGAAGAAACTTCAGGCGCCGGTGGAGTAGGGTTTGTTAAAGGTGTTGTGACATATATGATTATGGATGATTTGGAGGTGAAGCCTATGTCCACCATCTCCAGCATAACTCTTCTCAACAGGTTTAATGTCAAGGATGTGGGGGTTCTTCAAGAGAAGGTTGTTGATCTTGGCATGCAGGAGGGTGTGAAACTGTTGAAGGCGTCTCTTCAGTCGAAGTCGGTTCTGACGAGTGTGTTCATTGGGAAGAAAGCTGCTACATCACCTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

239

Amino Acids

25.52

Weight (kDa)

9.22

Isoelectric Point (pI)

40.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 7 - 232 3.9e-52 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000352)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g24890 FvH4_7g21280 FvH4_7g21300 FvH4_7g21300 FvH4_7g21310 FvH4_7g21330 FvH4_7g21340 FvH4_7g21600 FvH4_7g21610 FvH4_7g21630
malus_domestica MD07G1190400.v1.1 MD07G1190600.v1.1
prunus_persica Prupe.2G228600_v2.0.a1 Prupe.6G198200_v2.0.a1
pyrus_communis pycom07g18010
rosa_chinensis RchiOBHm_Chr1g0365711 RchiOBHm_Chr1g0365721 RchiOBHm_Chr1g0365731 RchiOBHm_Chr1g0365991 RchiOBHm_Chr5g0044511 RchiOBHm_Chr6g0247041 RchiOBHm_Chr6g0247081
rosa_laevigata RLG00000015223 RLG00000027405 RLG00000027406 RLG00000027407 RLG00000027408 RLG00000027409 RLG00000027411 RLG00000027413 RLG00000034277
rosa_multiflora Rmu_co8279495.1_g000001 Rmu_co8358963.1_g000001 Rmu_co8520123.1_g000003 Rmu_sc0002093.1_g000019 Rmu_sc0002093.1_g000024 Rmu_sc0002093.1_g000025 Rmu_sc0002093.1_g000029 Rmu_sc0002476.1_g000002 Rmu_sc0002476.1_g000021 Rmu_sc0003466.1_g000011 Rmu_sc0004288.1_g000006 Rmu_sc0004288.1_g000007 Rmu_sc0004311.1_g000022 Rmu_sc0004311.1_g000023 Rmu_sc0004311.1_g000026 Rmu_sc0004311.1_g000033 Rmu_sc0004577.1_g000008 Rmu_sc0004645.1_g000005 Rmu_sc0004645.1_g000006 Rmu_sc0006406.1_g000011 Rmu_sc0006406.1_g000012 Rmu_sc0009967.1_g000002 Rmu_sc0011598.1_g000003 Rmu_ssc0000151.1_g000015
rosa_roxburghii Rroxscaffold_1G00036500 Rroxscaffold_3G00276050 Rroxscaffold_4G00290950 Rroxscaffold_4G00290960 Rroxscaffold_4G00290970 Rroxscaffold_4G00290980 Rroxscaffold_4G00291000 Rroxscaffold_4G00291030 Rroxscaffold_4G00291060
rosa_rugosa Rorug01G0322900 Rorug01G0323000 Rorug01G0323100 Rorug01G0323200 Rorug01G0323300 Rorug01G0323400 Rorug01G0323500 Rorug01G0323600 Rorug05G0214700 Rorug05G0522100
rosa_samantha Rh1AG332000 Rh1AG332200 Rh1AG332300 Rh1AG334700 Rh1AG334800 Rh1AG335200 Rh1CG309900 Rh1CG310000 Rh1CG310100 Rh1DG325100 Rh1DG325300 Rh1DG325500 Rh5AG299000 Rh5BG306500 Rh5DG315800 Rh6AG037200 Rh6BG031900 Rh6BG032000 Rh6CG032300 Rh6CG032400 Rh6CG033200 Rh6DG030900
rosa_wichuraiana Rw0G001530 Rw1G019070 Rw1G029470 Rw1G029480 Rw1G029490 Rw1G029500 Rw1G029510 Rw1G029700 Rw5G027640 Rw5G027670 Rw6G003160 Rw6G003190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 262, 461
AccI GTMKAC 1 cut(s) 437
AccII CGCG 1 cut(s) 410
AclWI GGATC 2 cut(s) 267, 280
AcuI CTGAAG 4 cut(s) 53, 91, 441, 638
AcyI GRCGYC 2 cut(s) 462, 647
AflII CTTAAG 1 cut(s) 284
AflIII ACRYGT 1 cut(s) 327
AgsI TTSAA 2 cut(s) 596, 643
AjiI CACGTC 1 cut(s) 244
AluBI AGCT 6 cut(s) 18, 37, 80, 289, 377, 698
AluI AGCT 6 cut(s) 18, 37, 80, 289, 377, 698
Alw26I GTCTC 1 cut(s) 654
AlwI GGATC 2 cut(s) 267, 280
ApeKI GCWGC 1 cut(s) 698
Asp700I GAANNNNTTC 1 cut(s) 63
AspLEI GCGC 3 cut(s) 410, 412, 464
AspS9I GGNCC 1 cut(s) 367
AsuHPI GGTGA 3 cut(s) 423, 535, 699
AvaII GGWCC 1 cut(s) 367
AxyI CCTNAGG 1 cut(s) 258
BamHI GGATCC 1 cut(s) 272
BanI GGYRCC 2 cut(s) 262, 461
BbvI GCAGC 1 cut(s) 685
BccI CCATC 3 cut(s) 152, 394, 548
BcoDI GTCTC 1 cut(s) 654
BfaI CTAG 1 cut(s) 128
BfoI RGCGCY 1 cut(s) 465
BfrI CTTAAG 1 cut(s) 284
BisI GCNGC 1 cut(s) 699
BlpI GCTNAGC 1 cut(s) 290
BlsI GCNGC 1 cut(s) 700
Bme18I GGWCC 1 cut(s) 367
BmgBI CACGTC 1 cut(s) 244
BmgT120I GGNCC 1 cut(s) 367
BmiI GGNNCC 4 cut(s) 264, 274, 369, 463
BpmI CTGGAG 1 cut(s) 529
Bpu1102I GCTNAGC 1 cut(s) 290
Bsa29I ATCGAT 1 cut(s) 45
BsaHI GRCGYC 2 cut(s) 462, 647
BsaJI CCNNGG 1 cut(s) 363
BsaXI ACNNNNNCTCC 2 cut(s) 21, 51
Bse118I RCCGGY 1 cut(s) 464
Bse21I CCTNAGG 1 cut(s) 258
BseCI ATCGAT 1 cut(s) 45
BseDI CCNNGG 1 cut(s) 363
BseGI GGATG 5 cut(s) 163, 279, 386, 517, 586
BseMII CTCAG 2 cut(s) 281, 459
BsePI GCGCGC 1 cut(s) 408
BseRI GAGGAG 1 cut(s) 29
BseXI GCAGC 1 cut(s) 685
Bsh1236I CGCG 1 cut(s) 410
BshNI GGYRCC 2 cut(s) 262, 461
BshVI ATCGAT 1 cut(s) 45
BsiSI CCGG 1 cut(s) 465
BsmAI GTCTC 1 cut(s) 654
BsmBI CGTCTC 1 cut(s) 654
Bsp143I GATC 4 cut(s) 272, 321, 346, 610
Bsp1720I GCTNAGC 1 cut(s) 290
BspCNI CTCAG 2 cut(s) 282, 458
BspDI ATCGAT 1 cut(s) 45
BspFNI CGCG 1 cut(s) 410
BspLI GGNNCC 4 cut(s) 264, 274, 369, 463
BspPI GGATC 2 cut(s) 267, 280
BspT107I GGYRCC 2 cut(s) 262, 461
BspTI CTTAAG 1 cut(s) 284
BsrFI RCCGGY 1 cut(s) 464
BssAI RCCGGY 1 cut(s) 464
BssECI CCNNGG 1 cut(s) 363
BssHII GCGCGC 1 cut(s) 408
BssMI GATC 4 cut(s) 272, 321, 346, 610
BssNI GRCGYC 2 cut(s) 462, 647
Bst4CI ACNGT 5 cut(s) 25, 136, 233, 435, 639
Bst6I CTCTTC 4 cut(s) 16, 110, 561, 657
BstACI GRCGYC 2 cut(s) 462, 647
BstAFI CTTAAG 1 cut(s) 284
BstC8I GCNNGC 2 cut(s) 410, 620
BstDEI CTNAG 4 cut(s) 258, 290, 378, 445
BstF5I GGATG 5 cut(s) 163, 279, 386, 517, 586
BstFNI CGCG 1 cut(s) 410
BstH2I RGCGCY 1 cut(s) 465
BstHHI GCGC 3 cut(s) 410, 412, 464
BstKTI GATC 4 cut(s) 275, 324, 349, 613
BstMAI GTCTC 1 cut(s) 654
BstMBI GATC 4 cut(s) 272, 321, 346, 610
BstMWI GCNNNNNNNGC 1 cut(s) 121
BstNSI RCATGY 3 cut(s) 223, 331, 622
BstUI CGCG 1 cut(s) 410
BstV1I GCAGC 1 cut(s) 685
BstX2I RGATCY 1 cut(s) 272
BstYI RGATCY 1 cut(s) 272
Bsu15I ATCGAT 1 cut(s) 45
Bsu36I CCTNAGG 1 cut(s) 258
BsuTUI ATCGAT 1 cut(s) 45
BtrI CACGTC 1 cut(s) 244
BtsCI GGATG 5 cut(s) 163, 279, 386, 517, 586
BtsIMutI CAGTG 2 cut(s) 21, 297
Cac8I GCNNGC 2 cut(s) 410, 620
CfoI GCGC 3 cut(s) 410, 412, 464
Cfr10I RCCGGY 1 cut(s) 464
Cfr13I GGNCC 1 cut(s) 367
ClaI ATCGAT 1 cut(s) 45
CseI GACGC 1 cut(s) 636
CspCI CAANNNNNGTGG 2 cut(s) 406, 441
CviAII CATG 6 cut(s) 163, 220, 306, 328, 619, 714
CviJI RGCY 9 cut(s) 18, 37, 80, 145, 174, 289, 377, 529, 698
CviKI_1 RGCY 9 cut(s) 18, 37, 80, 145, 174, 289, 377, 529, 698
DdeI CTNAG 4 cut(s) 258, 290, 378, 445
DinI GGCGCC 1 cut(s) 463
DpnI GATC 4 cut(s) 274, 323, 348, 612
DpnII GATC 4 cut(s) 272, 321, 346, 610
Eam1104I CTCTTC 4 cut(s) 16, 110, 561, 657
EarI CTCTTC 4 cut(s) 16, 110, 561, 657
Eco47I GGWCC 1 cut(s) 367
Eco57I CTGAAG 4 cut(s) 53, 91, 441, 638
Eco81I CCTNAGG 1 cut(s) 258
EcoO109I RGGNCCY 1 cut(s) 367
EgeI GGCGCC 1 cut(s) 463
EheI GGCGCC 1 cut(s) 463
Esp3I CGTCTC 1 cut(s) 654
FaeI CATG 6 cut(s) 166, 223, 309, 331, 622, 717
FalI AAGNNNNNCTT 2 cut(s) 53, 85
FatI CATG 6 cut(s) 162, 219, 305, 327, 618, 713
FblI GTMKAC 1 cut(s) 437
Fnu4HI GCNGC 1 cut(s) 699
FokI GGATG 5 cut(s) 170, 266, 373, 524, 593
Fsp4HI GCNGC 1 cut(s) 699
FspBI CTAG 1 cut(s) 128
GlaI GCGC 3 cut(s) 409, 411, 463
GluI GCNGC 1 cut(s) 699
GsuI CTGGAG 1 cut(s) 529
HaeII RGCGCY 1 cut(s) 465
HapII CCGG 1 cut(s) 465
HgaI GACGC 1 cut(s) 636
HhaI GCGC 3 cut(s) 410, 412, 464
Hin1I GRCGYC 2 cut(s) 462, 647
Hin1II CATG 6 cut(s) 166, 223, 309, 331, 622, 717
Hin6I GCGC 3 cut(s) 408, 410, 462
HinP1I GCGC 3 cut(s) 408, 410, 462
HincII GTYRAC 1 cut(s) 438
HindII GTYRAC 1 cut(s) 438
HinfI GANTC 1 cut(s) 302
HpaII CCGG 1 cut(s) 465
HphI GGTGA 3 cut(s) 423, 535, 699
Hpy166II GTNNAC 2 cut(s) 438, 537
Hpy188I TCNGA 5 cut(s) 192, 315, 364, 448, 672
Hpy188III TCNNGA 2 cut(s) 149, 596
Hpy8I GTNNAC 2 cut(s) 438, 537
Hpy99I CGWCG 1 cut(s) 439
HpyAV CCTTC 2 cut(s) 595, 637
HpyCH4III ACNGT 5 cut(s) 25, 136, 233, 435, 639
HpyCH4IV ACGT 1 cut(s) 243
HpyCH4V TGCA 3 cut(s) 223, 622, 713
HpyF10VI GCNNNNNNNGC 1 cut(s) 121
HpyF3I CTNAG 4 cut(s) 258, 290, 378, 445
HpySE526I ACGT 1 cut(s) 243
Hsp92I GRCGYC 2 cut(s) 462, 647
Hsp92II CATG 6 cut(s) 166, 223, 309, 331, 622, 717
HspAI GCGC 3 cut(s) 408, 410, 462
KasI GGCGCC 1 cut(s) 461
Kzo9I GATC 4 cut(s) 272, 321, 346, 610
LmnI GCTCC 2 cut(s) 23, 42
LpnPI CCDG 9 cut(s) 28, 66, 127, 403, 444, 478, 550, 559, 608
Lsp1109I GCAGC 1 cut(s) 685
MaeI CTAG 1 cut(s) 128
MaeII ACGT 1 cut(s) 243
MaeIII GTNAC 2 cut(s) 233, 493
MalI GATC 4 cut(s) 274, 323, 348, 612
MboI GATC 4 cut(s) 272, 321, 346, 610
MboII GAAGA 7 cut(s) 3, 97, 461, 548, 584, 644, 703
MflI RGATCY 1 cut(s) 272
Mly113I GGCGCC 1 cut(s) 462
MmeI TCCRAC 2 cut(s) 7, 300
MnlI CCTC 7 cut(s) 19, 50, 135, 358, 454, 514, 619
MroXI GAANNNNTTC 1 cut(s) 63
MseI TTAA 4 cut(s) 252, 285, 483, 570
MslI CAYNNNNRTG 2 cut(s) 354, 712
MspCI CTTAAG 1 cut(s) 284
MspI CCGG 1 cut(s) 465
MvnI CGCG 1 cut(s) 410
MwoI GCNNNNNNNGC 1 cut(s) 121
NarI GGCGCC 1 cut(s) 462
NdeII GATC 4 cut(s) 272, 321, 346, 610
NlaIII CATG 6 cut(s) 166, 223, 309, 331, 622, 717
NlaIV GGNNCC 4 cut(s) 264, 274, 369, 463
NmuCI GTSAC 1 cut(s) 493
NspI RCATGY 3 cut(s) 223, 331, 622
PaeI GCATGC 1 cut(s) 622
PauI GCGCGC 1 cut(s) 408
PciI ACATGT 1 cut(s) 327
PcsI WCGNNNNNNNCGW 1 cut(s) 671
PdmI GAANNNNTTC 1 cut(s) 63
PfeI GAWTC 1 cut(s) 302
PflFI GACNNNGTC 1 cut(s) 92
PkrI GCNGC 1 cut(s) 700
PluTI GGCGCC 1 cut(s) 465
PpuMI RGGWCCY 1 cut(s) 367
PscI ACATGT 1 cut(s) 327
Psp5II RGGWCCY 1 cut(s) 367
PspN4I GGNNCC 4 cut(s) 264, 274, 369, 463
PspPI GGNCC 1 cut(s) 367
PspPPI RGGWCCY 1 cut(s) 367
PsuI RGATCY 1 cut(s) 272
PsyI GACNNNGTC 1 cut(s) 92
PteI GCGCGC 1 cut(s) 408
RseI CAYNNNNRTG 2 cut(s) 354, 712
SalI GTCGAC 1 cut(s) 436
SaqAI TTAA 4 cut(s) 252, 285, 483, 570
SatI GCNGC 1 cut(s) 699
Sau3AI GATC 4 cut(s) 272, 321, 346, 610
Sau96I GGNCC 1 cut(s) 367
SfoI GGCGCC 1 cut(s) 463
SgrAI CRCCGGYG 1 cut(s) 464
SinI GGWCC 1 cut(s) 367
SmiMI CAYNNNNRTG 2 cut(s) 354, 712
SmlI CTYRAG 1 cut(s) 284
SmoI CTYRAG 1 cut(s) 284
SphI GCATGC 1 cut(s) 622
SspDI GGCGCC 1 cut(s) 461
SspMI CTAG 1 cut(s) 128
TaaI ACNGT 5 cut(s) 25, 136, 233, 435, 639
TaiI ACGT 1 cut(s) 246
TaqI TCGA 5 cut(s) 45, 96, 150, 437, 659
TfiI GAWTC 1 cut(s) 302
Tru1I TTAA 4 cut(s) 252, 285, 483, 570
Tru9I TTAA 4 cut(s) 252, 285, 483, 570
TscAI CASTG 2 cut(s) 28, 304
TseFI GTSAC 1 cut(s) 493
TseI GCWGC 1 cut(s) 698
Tsp45I GTSAC 1 cut(s) 493
TspDTI ATGAA 1 cut(s) 673
TspRI CASTG 2 cut(s) 28, 304
Tth111I GACNNNGTC 1 cut(s) 92
Vha464I CTTAAG 1 cut(s) 284
VpaK11BI GGWCC 1 cut(s) 367
XceI RCATGY 3 cut(s) 223, 331, 622
XmiI GTMKAC 1 cut(s) 437
XmnI GAANNNNTTC 1 cut(s) 63
XspI CTAG 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.